Foss-Bactoscan FC PDF
Foss-Bactoscan FC PDF
Foss-Bactoscan FC PDF
The approved rapid method for determination of total bacteria in raw milk
Based on the measurement of Individual Bacteria Count (IBC), BactoScan™ FC+ gives an accurate determination of
the hygienic quality of raw milk with a capacity of testing up to 200 samples per hour. The FDA/NCIMS accepted
method delivers results in minutes, allowing farmers, milk testing laboratories and dairies to take action to preserve
and enhance hygienic quality of the milk supply.
Supported by a dedicated Foss IntegratorTM software, which provides a wide range of quality assurance and GLP
features. Foss Integrator shares the same interface for all CMT instruments.
Sample Parameters
• Milk types: cow, goat, sheep and buffalo milk Individual Bacteria Count (IBC/ml)
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BactoScan™ FC+ highlights:
• Automatic determination of raw milk hygienic quality
by Individual Bacteria Count
• Instant results in 9 minutes
• High capacity up to 200 samples/hour
• FDA/NCIMS approved
• Unique reagent concept
• Bacteria Control Sample for quality assurance and GLP
• Counting from 1500 CFU/ml to 10 mill CFU/ml
• Easy and safe to operate
• Superior routine method for payment analysis
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Technology
BactoScan™ FC+ is based on flow cytometry technology that Even the uncompensated carry-over is extremely low for the
enables precise and instant milk bacteria analyses. BactoScan™ FC+ and proven to be below 0.5%, which is
superior to the 1% required by the ISO/IDF. A carry-over test
Flow cytometry is a technique that counts bacteria cells. The is a part of the quality assurance and checks that the instru-
principle behind flow cytometry is quite simple: a suspension ment is able to purge a sample completely through the system
of cells is stained and forced through a capilary tube, which is without affecting the next sample. The test complies with the
illuminated in front of microscope objective. Every passing cell ISO/IDF standard.
is registered by photo electronics attached to the microscope.
IBC to CFU conversion
Prior to measurement all components in the milk, except for
BactoScan™ FC+ measures Individual Bacteria Count and
the bacteria, are broken down during an incubation period.
displays results in IBC. However, many laboratories are obliged
Additionally, the bacteria clusters are separated into single
to present their results in Colony Forming Units (CFU), which
bacteria, which are subsequently dyed with DNA specific stain-
can be obtained by a plate count method. Consequently, a
ing medium, ethidium bromide. All the reagents involved are
conversion table has to be created to convert from IBC to CFU.
automatically filtered directly prior the application to eliminate
the risk of contamination from other sources than the milk.
The BactoScan™ FC+ software includes a Guided Conversion
Tool – an easy and rapid software tool to develop a robust
conversion table between IBC and CFU
Avoiding carry-over
Conversion graph: IBC/CFU
FOSS has developed a unique self-cleaning program to limit
carry-over contamination from previous samples. Optionally,
the effect of carry-over between the samples may be reduced “BactoScan™ FC+ carry-over below 0.5%which
by a mathematical compensation that is made automatical-
is superior to 1% required by the ISO/IDF”
ly. Such compensation is based on observed carry-over be-
tween the samples and can be deducted from the results.
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Special feature for difficult milk samples applied on regular basis, the milk standard plays a crucial role
in quality assurance.
Options are available to handle milk samples from, for example,
sheep and goat which can be subject to high concentrations
of somatic cells - like in goats milk at the end of lactation. This Easy reagents handling – in minutes with
induces background noise leading to high readings on the highest security
BactoScan™ FC+ – often due to increased viscosity of the milk The unique reagent concept ensures simple, safe and very fast
sample, which in turn will make the flow of the sample film reagents handling, which reduces the operator’s dependency.
through the flow cell unstable. The influence of the background Low reagents consumption and low waste contribute to the
noise typically starts > 4 mill. somatic cells/ml. environmental protection. By the process of internal filtration
of reagents, the time is saved and the risk of contamination
The Enhanced mode option stabilizes the flow of the film is eliminated.
through the flow cell. The measuring speed of milk will be
reduced by either 50% with the mode 1, or 75% with the Unique reagent concept:
mode 2 without affecting the speed capacity of the instrument.
• Quality assured with certificates for all reagents*
• Simple, fast and safe mix of reagents
Foss Integrator software platform • Minimum time used for reagent preparation
Foss Integrator is a shared software platform supporting other and less operator dependency
FOSS milk testing solutions. The same user interface, the same • Low reagent consumption and low waste for environmental
conveyors, bar code readers etc. can be used throughout the protection
laboratory. This eases training of laboratory staff and facilitates • Low reagent costs per sample
data handling, transfer and storage. Uniform software provides • Internal filtration of reagents prior to use, saves time
flexibility in the laboratory. and removes risk of contamination
Further, context sensitive help available via Foss Integrator *Available at the FOSS homepage
software provides easy and quick solutions and answers, as
well as provides an excellent tool for quality assurance.
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Specifications
Performance
Repeatability*:
Range (IBC/µl) SR (log-units) Typical SR (log units)
10 – 50 0.07 0.06
51-200 0.05 0.04
>200 0.04 0.02
Entire range 0.05
Installation requirements
Feature Specification
70 x 140 cm (without conveyor)
Dimensions 85 x 195 cm (with Basic Conveyor*)
85 x 260 cm (with Conveyor 4000*)
197 kg (without conveyor)
Weight 205 kg (with Basic Conveyor*)
250 kg (with Conveyor 4000*)
Space requirements Approx. 2 x 4.5 meters
Power supply 110 - 240 V AC
Power consumption 50VA stop, max 2000 VA
Water supply for preparation Purified water (<5 μS/cm3)
Air (for Conveyor 4000*) 0.2 N litres/min at 4.0 – 7.2 bar
Waste Approx. 8 litres per hour
Ambient temperature 15 – 33 °C (59-91.4 F)
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Application data
Capacity BactoScan FC+:
Models: 65H, 130H, 200H = 65, 130, 200 samples per hour
Sample quality: Raw milk of normal composition and good quality. Unpreserved or preserved with azidiol
Data output
Real-time display/print-out, storage on hard disk.
Host transmission (RS232) and PC network transmission (TCP/IP).
Data export using CSV files, CS83 protocol or XML.
Standard equipment
Basic analyser incl. table and reagent containers, PC, software, Conveyor 4000, Basic Conveyor*.
Optional equipment
Printer, extra reagent containers, *ID bar code laser scanner, *2D reader, *ID bottle rotation, *Conveyor 4000, *Conveyor
extensions, *Output buffer, *Sample racks.
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P/N 1026630, Issue 1 GB, May 2013
FOSS
Foss Allé 1
DK-3400 Hilleroed
Denmark
info@foss.dk
www.foss.dk