DEPARTMENT OF SCIENCE AND TECHNOLOGY

BIOTECHNOLOGY
R&D ROADMAP
(2013-2019)
AGRICULTURE
AGRICULTURE : CROPS Competitive and
ABACA and COCONUT Sustainable Agriculture
and Fisheries Sectors
for Food Security and
COCONUT: Increased Productivity
M.M. for early flowering, fast growth, oil and nut yield and water content and quality (JAN 14, 2019)[3]
Varieties from M.A. breeding of Tall x Tall and Tall x Dwarf (JAN 14, 2019)[7]
Genetic linkage map derived from validated QTLs for routine M.A. breeding (JAN 14, 2019)[8]
Markers and NGS-Eco TILLING platform for glandular trichome genes; SNP markers for coconut scale COCONUT:
 Estabm’t of breeding population [3]
insect resistance gene (JAN 14, 2019)[9]
 Dev’t of robust markers for routine M.A. breeding [8]
COCONUT:  Eval’n of plant/s that exhibit differential reaction
M.M. for dwarf variety, developmental processes, oil and lipid production, and metabolic against scale insect infestation [9]
processes (JAN 14, 2017)[2] COCONUT:
Transcriptome sequence of 8 coconut varieties & putative gene markers for early Dev’t of linkage map early flowering, oil, nut yield, H2O content [3]
flowering, fast-growing, high nut yield and water content & quality (JAN 14, 2017)[4]
M.M. for oil biosynthesis of high oil-yielding, makapuno Ident’n of early flowering, high nut yield, H2O content & quality[4]
& lono coconuts (JAN 14, 2017) [5]
Dev’t of M.M. for use in M.A. selection and breeding [5]
Genes involved in metabolic pathways & oil  Regeneration of transformed tissues into whole plantlets in bottles [6]
biosynthesis (JAN 14, 2017) [6]  Dev’t of composite/synthetic varieties from M.A. breeding [7]
 Mapping of QTLs of productivity & yield/quality of copra oil & by-products [8]
 NGS-Eco TILLING platform for glandular trichomes genes [9]
Dev’t of markers for dwarf variety, developmental processes oil & lipid prod’n & metabolism[2]
Dev’t of primers for t M.A. breeding, hybridity testing, forensics, fingerprinting, genotyping [3]
Dev’t of draft transcriptome sequences of the 8 coconut varieties & putative gene markers [4]
COCONUT:Comparison of listings and Identificat’n of candidate genes and relevant gene networks [5]
Dev’t of at least 5 constructs with one or two genes for oil biosynthesis [6]
Selection & nationwide replanting of genuine PCA hybrid seed nuts & 'San Ramon' composite variety [7]
Dev’t of genetic linkage map of coconut for High Yield and Outstanding Quality of Copra Oil [8]
Charact’n of coconut glandular trichome loci/genes tagged with sequence-specific DNA markers[9]
ABACA: M.M. for fiber ABACA: Identificat’n of genes for fiber quality & COCONUT:
ABTV resistance and construction of genomic Generating contig assemblies of the transcriptomes of normal,
quality & virus resistance DNA library/ database[1] makapuno and lono phentoypes [5]
(APRIL 30, 2014)[1] COCONUT: Gene discovery of at least 5 coconut genes involved in several
Establishment of biomarkers for early flowering, fast growth, metabolic pathways [6]
oild & nut yield and water content & quality. [3] Dissemination of Outstanding Tall coconut selections in target
Genomic sequencing of Laguna Tall (LAGT) and Catigan growing regions based on genetic relationships [7]
Green Dwarf (CATD) of Cocos nucifera [2] Dev’t of polymorphic DNA markers bet. parental populat’ns [8]
Purification of total RNA from 8 rep coconut varieties [4] Construct’n of web-based genome database of Cocos nucifera [9]
ABACA: Development of SSR based primers from the NGS Abuab and gDNA library [1]
 AGRICULTURE : CROPS
Competitive and Sustainable
COFFEE, SUGARCANE, RICE, RUBBER and CACAO Agriculture and Fisheries
Sectors for Food Security
and Increased Productivity
RUBBER & CACAO : Validated PCR primers for possible two (2) trait association (JAN 2018)[19]

RUBBER & CACAO : Designed primers (DEC 2017)[19] RUBBER & CACAO : Selection of SSR
markers from exome databases for primer
SUGARCANE: COFFEE: M.M. for pest/disease design (gene mining) [19]
M.M. for High Sucrose Content, resistance,yield & aroma (OCT 2016)[10]
Resistance to Smut & Resistance to RICE:
Downy-Mildew (AUG 16, 2016)[11] Technology on Radiation- RUBBER & CACAO: Sequencing of 200 colonies, identified
Protocol of M.A. nobilization for Modified Carrageenan & SSRs from sequenced colonies and designed primers; test
improvement (AUG 16, 2016)[12] Chitosan Rice management designed primers to the 10 NSIC varieties [19]
5 New Improved Varieties with high (MAY 16, 2016)[14]
sucrose content & resistance to fungal Varieties resistant to tungro
diseases through MAS (AUG 16, disease & best pest treatment  RICE: Testing the induction of resistance in 3 susceptible hybrid rice varieties &
2016)[13] (MAY 16, 2016)[15] Field experiments and advance evaluation in farmers field [15]
 RUBBER & CACAO: Prep’n for 2 gene-enriched gDNA libs & Isolation of 100
RUBBER: transformed bacterial colonies [19]
Validated 185 rubber nurseries in North  SUGARCANE:
Cotabato, Zamboanga Peninsula, Caraga,  Testing and validation of some of these markers in sugarcane breeding populations/ lines[11]
Sultan Kudarat, Palawan, Quezon,Kalinga  Evaluation of the effectivity of markers in identifying the true hybrids [12]
Apayao, & Nueva Viscaya (MAR 2015)[16]  Field testing of promising varieties [13]
gDNA libraries (APRIL 2015)[19] Determt’n of the effect of chitosan and carrageenan  SUGARCANE:
CACAO: Gene-enriched gDNA libraries application on rice growth, nutrient uptake, and Const’n of RNA sequence data for mined
for cacao (APRIL 2015)[19] physiological process [14] information and database [11]

Testing Induction of resistance, dosage refinement, and traits & interspecific forwith
Identf’n of accessions markers for most
frequency of application [15] parental purposes [12]
 COFFEE: Identf’n of M.M. for resistance to pests (berry borer & scale insect), Identf’n of promising sugarcane clones with high
sucrose content, and resistant smut and downy
diseases (leaf blight & rust), yield, and aroma of NSIC varieties & strains [10] mildew through the use of MAS [13]

Extract’n of total RNA & generation of transcriptomics sequence data using a  RUBBER: Baselined rubber
appropriate NGS Platform [11] plantations/nurseries with high yielding
DNA Fingerprinting of entries using the established markers for sucrose content, smut rubber clones from the target
and downy mildew) to produce a molecular database of the varieties [12] nurseries, Identification of clones and
Evaluation of the clones through Brix and morpho-agronomic characteristics [13] SSR analysis [16]
AGRICULTURE : CROPS
MANGO, SQUASH and TOMATO High Yielding and Pest & Diseases
Resistant crops
TOMATO:
Open-pollinated varieties and hybrids of fresh-market and processing resistant to ToLCPHV (2019)[22*] TOMATO: M.A.
Genetic population/s of a fresh-market & processing for the target disease resistance and abiotic stress tolerance traits (2019)[22*] breeding for leaf
curl virus resistant
TOMATO: (2019)[22*]
Genomics-assisted mapping &
 Commercial release of virus resistant tomato(2018)[22*] pyramiding of disease resistance
 Spin-off negotiations with local & intern’l breeding institutions/seed companies (2018)[22*] & abiotic stress tolerance genes
 Genome charact’n & extent of genetic diversity among ToLCPHV species in the Philippines (2018)[22*] (2019)[22*]

MANGO: Database of morphological and molecular characteristics (2017)[18*] TOMATO: Multi-location field trial and Plant
Variety Protection (PVP) filing towards
commercial varietal release and Genomics &
MANGO: M.M for red blush, thick peel, resistance to insect pests (fruit fly, cecid fly, leaf variability of Tomato leaf curl Philippine [22*]
hopper) and major diseases (anthracnose and stem-end rot) (2016)[18*]
 MANGO: Ident’n of M.M. in varieties associated
SQUASH: One (1) improved population and 1 open-pollinated variety with key traits [18*]
moderate resistance to SLCV, ZYMV, and PRSV-W (MAR 2015)[20]
TOMATO: DNA markers tagging the virus resistance (MAR 2015)[21]  MANGO: Re-evaluation of mango collections with peel color, thickness, and
MANGO: Diagnostic kit (dipstick kit) for Ident’n of ‘Carabao-Mango’ resistance to anthracnose, stem-end rot, fruit fly, cecid fly, leaf hopper [18*]
(FEB 28 2015)[17]

MANGO: DNA markers for ‘Carabao-  MANGO: Testing of the diagnostic kit (dipstick kit) in mango nurseries [17]
Mango’(FEB 28 2014)[17]  SQUASH: Dev’t of populations resistant to SLCV, ZYMV & PRSV-W [17]
SQUASH: Six (6) germplasm resistant to  TOMATO: Dev’t of DNA markers tagging the viral resistance gene sequences [21]
SLCV, ZYMV & PRSV-W (MAR 2014)[20]
TOMATO: Characterized virus resistance  MANGO: Ident’n of ‘Carabao’ mango specific proteins for dev’t of diagnostic kit (dipstick kit [17]
(MAR 2014)[21]  SQUASH: Identify sources of genetic resistance to SLCV, the Zucchini yellow mosaic virus (ZYMV),
and the Papaya ringspot virus type W (PRSV-W) in collaboration with AVRDC [20]
SQUASH: One (1) DNA clone for  TOMATO: Conduct molecular characterization of induced resistance gene sequences [21]
predominant strain of SLCV
(MAR 2013)[20]
 MANGO: Development and identification of DNA molecular markers, database of molecular characteristics and population
TOMATO: Allelic variant structure of Philippine mango cultivars [17]
resistant to TYLCV/ToLCV  SQUASH: Determination of predominant strains of squash-infecting Begomovirus and development of infectious DNA
& CMV (MAR 2013)[20] clones of local strains of the major squash virus, the Squash leaf curl virus (SLCV) [20]
* Proposed Project  TOMATO: Screening the loss of susceptibility mutants of tomato against TYLCV/ToLCV, CMV [21]
 AGRICULTURE : LIVESTOCK & POULTRY Competitive and Sustainable
GOAT, WATER BUFFALO, SWINE, POULTRY and MEAT PRODUCTS Agriculture and Fisheries Sectors
for Food Security and Increased
Productivity
WATER BUFFALOES: Equation for predicting genetic merit of animals based on BLUP EBVs and GEBVs and
Selection index for bulls and heifers incorporating BLUP EBVs and GEBVs. (NOV 30, 2017)[7] WATER BUFFALOES: Dev’t
of model for predicting
MEAT PRODUCTS: DNA barcode sequence of 12 animal species) (MAR 31 2016)[4] genomic breeding values for
SWINE: Policy brief to institutionalize the adoption of LAMP assays by state-based animal disease diagnostic marker genotypes using
laboratories (JUN 30 2016)[6] Valid’n of prediction eq’n [7]
WATER BUFFALOES: genetic architecture of the Phil. dairy population (JUN 30 2016)[6]
SWINE:  MEAT PRODUCTS: Testing & Valid’n of
PCR and LAMP based methods [4]
Genetic data on the presence or absence of positive/negative genes (DEC 31, 2015)[3]  SWINE: Pilot scale at capacity of 300
Packaged LAMP test kit for PED virus (for pilot scale production) (JUN 30 2014)[6] units/yr & prod’n of PED LAMP test kits [6]
MEAT PRODUCTS: 4 sets of species-specific LAMP primers & 12 pairs of species-specific PCR  WATER BUFFALOES: Genome ass’n studies,
primers (MAR 31 2015)[4] comparison of models & Principal component analysis [7]
SWINE & POULTRY: PECM as a new feed product (DEC 31, 2015)[5]
WATER BUFFALOES: SNP markers for milk production traits (DEC 15, 2015)[7] SWINE: Field validt’n of MEAT PRODUCTS: Dev’t of LAMP-based
species ident’n protocol (4 species)[4]
GOAT: LAMP primers and Phylogenetic Analyses for CAEV, (JUL 16 2014)[1] PED in Reg. 3 & 4 [6]  SWINE & POULTRY: Eval’n of PECM in
SWINE:  Ass’n analysis of genotype w/ commercial swine and poultry farms [5]
phenotype & Establ’t of genetic WATER BUFFALOES: DNA sampling,
LAMP Protocols for detection of PED virus, Salmonellosis & service laboratory [3] extraction, genotyping & data eval’n [7]
Cryptosporidiosis & LAMP test kit for PED virus (SEPT 30 2014)[2]
Gene markers for ident’n of positive & disease resistance/ MEAT PRODUCTS: Optim’n of PCR-  SWINE:
screening of genetic defects(DEC 31 2014)[3]
LAMP Protocols respiratory & gastrointestinal diseases based species ident’n protocol (for 12
animal species) for fresh meat & meat
Opt’n of LAMP protocols, Dev’t & valid’n
via “lab in a mug” heat blocks (JUN 30 2014)[6] of LAMP-based quick test kit [2]
SWINE & POULTRY: Bioprocessing technology for
products [4]  Blood sample coll’n, DNA extraction and
protein enrichment of copra meal (DEC 31 2014)[3]
GOAT: Measurement of efficiency, reliability & Genotyping of DNA samples to identify
validation of the designed LAMP assay for CAEV positive, disease resistance genes and
MEAT PRODUCTS: LAMP ident’n protocols & test followed by the Charact’n/testing of CAEV [1] screen genetic defects [3]
kits (MAR 31 2014) [4]
 SWINE & POULTRY: Testing & optimization of operating condition  Re-optimization of PED LAMP protocols
using fabricated “Lab in a Mug” heat
SWINE: LAMP primers for PED virus, in each of the steps in the prod’n system and Establ’t of product
(PECM) quality data & analysis [5] block [6]
Salmonellosis & Cryptosporidiosis
(SEPT 30 2013)[2]
GOAT: LAMP Protocol Design of LAMP primers & for PEDV, Salmonellosis, Cryptosporidiosis [2]  GOAT: Primer design of the CAEV strains
for the detection & Ident’n & optim’n of gene primers of positive traits (litter size, growth rate, and field validation. [1]
screening of CAEV meat quality, muscle mass), disease resistance (salmonellosis, PRRSV,  SWINE & POULTRY: Estab’t of pilot scale
(JUL 16 2013)[1] intracellular pathogen, E. coli and influenza) & screening of genetic defects prod’n system for protein enrichment of
(acid meat, stress syndrome and scrotal hernia) [3] copra meal [5]
 AGRICULTURE : INLAND AQUATIC Competitive and Sustainable
MILKFISH, MUSSEL SHRIMP and TILAPIA Agriculture and Fisheries Sectors
for Food Security and Increased
Productivity

SHRIMP:
Standard protocol for dsRNA mass production (JUN 2016)[9]
Protocol on selection/sourcing of P. monodon seedstock (JUN 2016)[10]
MUSSEL: M.M. for higher survival, faster growth & disease resistance (2015) [5]  MUSSELS: Identif’n of stains/varieties/ populations
for selective breeding and generate a genetic profile
MILKFISH: for the natural populations of this species. [5]
M.M. of best stock for breeding & distinction of wild fry vs. hatchery-bred fry (MAY 2015)[1] SHRIMP:
Procedure using fish scales to measure/detect early growth rates (MAY 2015)[2]  Dev’t & eval’n LFSB prototype performance for WSSV
M.M. for lectin to assess innate immunity (MAY 2015)[3] screening & field test trials and Dev’t of Nested PCR protocols
Diagnostic kit for Microbial Infection (MAY 2015)[4] for the detection of WSSV, IHHNV, MBV and Vibrio spp; [8]
SHRIMP:  Utilization and innovation of databases/methodologies and dev’t of
Genome sequence of 300 shrimp pathogens (DEC 2015)[6] local applications to control and/or neutralize WSSV [9]
Online Philippine Shrimp Pathogen Biobank (DEC 2015)[7] Utilization of NGS & bioinformatics to conduct “association studies” to identify a
Diagnostic kit - Lateral Flow Strip Biosensor (LFSB) (DEC 2015)[8] gene or a group of genes in tiger shrimp related to high growth, high health,
TILAPIA: disease tolerance etc [10]
Comparison of Genetic Variation of Philippine Tilapia & Other
countries (i.e. China, Thailand & Malaysia) (SEP 2015)[11]
Profile of hatcheries: strain, management applied, maturity  MUSSELS: Determt’n of genetic variability from different sites using RAPDs, RFLPs
status and production (SEP 2015)[12]  TILAPIA: and unique regions of specific genes [5]
 Examination of levels of genetic diversity in  MILKFISH:
improved strains of Nile and red tilapia [11] Ident’n of highly genetically variable stocks
MILKFISH: Populations with high  Generate scientific data of varieties (GIFT, for use in stock replenishment [1]
genetic variability (MAY 2014)[2] FAST, GET-EXCEL, SEAFDEC, GST Design gene specific primers based on
 SHRIMP: (GenoMar Supreme Tilapia). [12] teleost lectin sequence databases & peptide
 Sequencing of 300 pathogens from outbreaks & the field [6] sequences 
[3]
 Establ’t of an online Philippine shrimp pathogen database [7] for a rapid and
Dev’t of a molecular diagnostic technology
 Synthesis of gold metal nanoparticles-DNA conjugates & reliable detection of
pathogenic species [4]
incorporation into a lateral flow strip biosensor (LFSB) [8]
MILKFISH:
Dev’t of microsatellite and RAPD markers for evaluating the impact of domestication on local milkfish hatchery stocks [1]
Sequencing of mitochondrial cytochrome b gene and control region [2]
Cloning & sequencing of lectin genes from a pooled cDNA library from the liver, kidney, and spleen [3]
Utilization of advanced molecular technologies to identify & detect pathogenic bacterial species from diseased fish samples [4]
 AGRICULTURE : MICROBIALS for crops Competitive and Sustainable
MYKOPLUS: Established profitability of MykoPlus (MAY 31 2015)[1] Agriculture and Fisheries Sectors
BACTERIAL INOCULANT: Established profitability of Inoculant (MAY 31 2015)[2] for Food Security and Increased
BIOFERTILIZER: Established profitability of Biofertilizer(MAY 31 2015)[3] Productivity
STIMULANTS: Established profitability of stimulants (MAY 31 2015)[4]
BIOCONTROL: Established profitability of Biocontrols (MAY 31 2015)[6]
BIOPESTICIDE: Most effective carrier forml’n & Established profitability (MAY 31 2015)[7]
BIOBANK: M.M. of the new BFSP strains & preserved strains for long-term use & distribution for  MYKOPLUS: eterm’n of the economics of using MykoPlus [1]
researchers (MAY 31 2015)[8]  BACTERIAL INOCULANT: Bio-efficacy & Field Testing the
effectiveness of formulated EB in the field; Cost- benefit
BACTERIAL INOCULANT: Fertilizer w/ EB inoculant (MAY 31 2014)[2] analysis for using the developed product(s) [2]
BIOFERTILIZER: Info on soil microbial diversity of the acid soil envi. (MAY 31 2014)[3]  BIOPESTICIDES:
STIMULANTS: Fertilizer using chemical fertilizer & biostimulant (MAY 31 2014)[4]  Eval’n of the formulated biopesticide and Cost & return analysis [ 5]
BIOCONTROL: Application mode of Biocontrol agent (MAY 31 2014)[6]  Efficacy trials of biocontrol agents & Economic [ 7]
BIOBANK: New strains isolated with BFSP properties (MAY 31 2014)[8]  BIOCONTROL: Biomass recovery, formulation & storage stability tests,
MYKOPLUS: Increased awareness & adoption and Business plan for Laboratory, greenhouse and field tests; Cost benefit analysis [6]
MykoPlus technology (MAY 31 2014)[1]
BIOPESTICIDE:  BIOBANK: Dev’t of M.M. accession of strains in the PNCM culture holdings [8]
Formulated biopesticides (MAY 31 2014)[5]  BIOFERTILIZER: Cost-benefit analysis on the appl’nn of the new microbial inoculant [3]
Toxicological and environmental impact assessment  STIMULANTS: Forml’n of microbial phytohormone-based stimulant, eval’n of product
with biocontrol agent application (MAY 31 2014)[7] effects & economic analysis on the use of biostimulant product [4]
STIMULANTS: Microbial phytohormone-based  BIOPESTICIDES:
biostimulant (MAY 31 2013)[4]  Isolation, charac’n & forml’nn of pesticidal compounds from callus-microbe co-cultures [5]
BIOCONTROL: Yeast for postharvest pathogens  Toxicological eval’n & microbial community population dynamics study as affected by the
of fruits & vegetables (MAY 31 2013)[6] introd’n of the biocontrol agents [7]
BIOBANK: Characterized Strains & re-identified  MYKOPLUS:Quantification of the effect and soil amendments/properties on growth and yield [1]
taxonomic methods (MAY 31 2013)[8]
BIOPESTICIDE:  BACTERIAL INOCULANT Formul’n & shelf life assessment of EB inoculant as new biofertilizer [2]
Prod’n Process of biopesticides by callus &  BIOFERTILIZER: Formul’n of a consortium of microorganisms & carrier materials & Field testing [3]
 STIMULANTS: Screening of locally available materials as substrate for phytohormone production [4]
microbe co-culture (MAY 31 2013)[5]
Microbial isolates w/ biological control  BIOBANK: Charac’n & Polyphasic identif’n of strains, biofertilizer, biostimulant & biopesticide [6]
action (MAY 31 2013)[7] BIOPESTICIDES:
BIOFERTILIZER: Multi-strain Screening for pesticidal compounds from callus microbe co-cultures[5]
biofertilizer (MAY 31 2013)[3] Isolation & screening of microbes with presumptive biocontrol action to causal agent of banana Fusarium wilt &
bacterial blight of corn (Rhizoctonia solani) & forml’n of the effective biocontrol agents[7]
BACTERIAL INOCULANT:  MYKOPLUS (for Yellow Corn & Eggplant): Establishment of demonstration trials and trainings on application [1]
Inoculant in Powder Form  BACTERIAL INOCULANT (for Eggplant & Sugarcane): Morphological, biochemical & molecular characterization of EB isolates [2]
(MAY 31 2013)[2]  BIOFERTILIZER: Assessment of the rhizobacterial dynamics in acid soils & Testing of isolates for plant growth promotion [3]
MYKOPLUS:  STIMULANTS: Construction of phytohormone-hyper-producing consortia for potential phytohormone prod’n [4]
Mykoplus Primer  BIOCONTROL: Isolation and identification of yeasts with potential antagonistic activity [6]
(MAY 31 2013)[1]  BIOBANK: Preservation of cultures [8]
ENVIRONMENT
& NATURAL RESOURCES
ENVIRONMENT & NATURAL RESOURCES : Conservation of Philippine
Biodiversity Conservation and Bioremediation Biotech Biodiversity for Bio-remediation,
Industrial, Health and Agricultural
Database for conservation, source of high quality seeds, potential industrial uses bioremediation technologies Applications
for contaminated wastewater and soil
Field-based, gene-based conservation programs for identified aquatic, marine and terrestrial species
Biotechnology based ecological restoration of polluted sites
Development of policy recommendations on bioprospecting
Genetic varieties of endemic species, ecotourism flora and fauna Development of policy
recommendations on
Field-based, gene-based conservation programs for identified aquatic, marine and terrestrial species bioprospecting
Biotechnology based ecological restoration of polluted sites
Review of regulations on bioprospecting
 Review of regulations on bioprospecting
Compound /enzymes for bioremediation technologies,industrial, health & agricultural applications  Field or gene-based conservation programs
for identified aquatic/terrestrial species
National Greening Program for reforestation project; database for conservation,  Biotechnology based ecological restoration
source of high quality seeds, potential industrial uses of polluted sites
Biosensor & test kits, for marine toxins photo bioreactor, biofertilizers, biofilters,
identified species for bioremediation, marine detrivores & filter feeders
Genetic & hydrodynamic connectivity patterns within archipelago and region, Pilot scale and field testing of compound and enzyme
Baseline genetic diversity & resilience estimates for selected indicator species production for bioremediation technologies and industrial,
health and agricultural applications
Field-based correlation of gene-markers & best properties of identified species
Gene products from identified M.M. for Isolates microbial culture collection
Compounds and enzymes from best genetic flora & fauna varieties
Harmful algal blooms detection  Isolation, identification & functional characterization of compounds, enzymes & best
genetic flora & fauna varieties
Microorganisms effective in accumulating heavy metals  Field-based correlation of gene-markers and best properties of endangered
and capable of biofilm formation (Feb 14, 2014)[1] species/varieties.
Compounds & enzymes from best genetic varieties
propagation protocol
Screening, Isolation, identification & functional characterization of compounds,
Bacteria forming Biofilms for heavy enzymes & best genetic varieties
metal absorption (Dec 31, 2013)[2]
Key genes of plants, animals
& microorganisms for  Gene sequencing design, run & data analysis, identification of key genes of plants, animals & microorganisms
conservation, disaster
mitigation & remediation for conservation, Disaster mitigation and remediation
Database of identified  Identification of the microorganisms effective in accumulating heavy metals and capable of biofilm formation [1]
species and their genome  Screening & Identification of Biofilm Formers as Potential Microbial Remediators for Heavy Metal Contaminated Wastewater [2]
ENERGY
ENERGY: BIOFUELS
Efficient and Sustainable
Alternative Fuel Source
Performance data at bench scale
Algal based bioethanol using improved strains Pilot-scale genetically
Algal based biodiesel using improved microbial engineered microbial
strains production of ethanol &
lactic acid for biofuels.

Optimized Conditions for Pretreatment, Saccharification Energy biomass & substrate

& Fermentation of Lignocellulosic Feedstocks (Mar 31, optimization for ethanol & lactic acid
2015)[1,3] production for biofuels
Identified Microbial Strains for bioethanol from
a. Lignocellulosic feedstocks (Mar 31, 2015)[2]
b. Sugar-based feedstocks Small-scale genetically engineered microbial
c. Macroalgae production of ethanol for biofuels

Nanostructrure of microbial strains - Microbial strains for

biodiesel from microalgae Genetic engineering of microorganisms to express enzymes that
produce ethanol for biofuels

Isolation, chemical, biochemical & functional characterization of genes, small

molecules & enzymes for bioenergy

Gene sequencing design, run & data analysis, & identification of key genes of marine & microbial
samples for bioenergy.
Identification and evaluation of appropriate enzymes and the appropriate pretreatments, saccharification and
fermentation conditions for specific lignocellulosic feedstocks[1,3]
Development of Microorganisms Capable of Utilizing Lignocellulosic Hydrolysates for Fuel Ethanol Production[2]
HEALTH
HEALTH
Harnessing Biotechnology to
Boost National
Competitiveness in the
Development of Health Care
Solutions and Services for
Validation of published mutations in the KRAS, PIK3CA, BRAF, PTEN and AKT1 genes in Filipino colorectal cancer Inclusive Growth
patients through next generation sequencing technology. (Dec 2017) [5]
Detection of novel mutations in colorectal cancer tumors that can be further characterized in order to determine their  Identification of mutations in the KRAS,
molecular and biological functions in cancer development. (Dec 2017) [5]
PIK3CA, BRAF, PTEN and AKT1
genes will be determined via targeted
Validated and stable Lateral POC system for the detection of dengue (June 30, 2014)[1] 
sequencing (Colorectal Cancer) [5]
Genomic SNP markers for cardiovascular conditions, and responses to treatment for Assessment of the mutations in the
KRAS, PIK3CA, BRAF, PTEN and
cardiovascular diseases (June 2016) [3] AKT1 genes using various mutational
SNPs Database for cardiovascular disease, hypertension and dyslipidemia (June 2016) [3] assays (Colorectal Cancer )[5]
Biomarkers in the form of genetic and transcriptomic biomarkers that are predictive of T2DM,
related medical conditions, complications and of clinical responses to various treatment (Mar
2016) [4] Small scale validation, stability testing and community-
These markers can be translated to diagnostic kits to identify at-risk individuals who 
based testing of the POC kit [1]
Genotyping for Type 2 Diabetes Mellitus, SNP Screening,
may require special and tailored interventions and/or counseling (Mar 2016) [4]
A descriptive database for the prevalence of these SNPs among Filipinos (Mar Processing of samples for microarray procedures [4]
2016) [4]  Genotyping, SNP Screening, Processing of samples for microarray
procedures (Hypertension, Coronary Artery Disease & Dyslipidemia) [3]

Biotek-M Dengue-seco Kit (Lyophilized  Extraction of DNA, processing and documentation of samples for quantity & purity
Format) (March 30, 2015) [2] (Hypertension, Coronary Artery Disease and Dyslipidemia) [3]
 Extraction of DNA, processing and documentation of samples for quantity & purity
(Type 2 Diabetes Mellitus) [4]
 Optimization of Biotek-M dengue-seco kit , Stability testing of Biotek-M dengue-seco kit [2]
Prototype of the Lateral POC Sytem (Dec  Comparative laboratory performance testing between dengue-seco against the liquid format [2]
31, 2014)[1]
Data on the advantage of MODS Assay in  Production, screening and characterization of immuno reagents for the lateral flow test [1]
terms of rapid production of test results
and major advantage in terms of  Design and development of a Lateral POC System[1]
performance, efficiency, and cost  Patient recruitment, sample collection & baseline data collection for 2,850 participants (Hypertension, Coronary
effectiveness (March 2014)[1] Artery Disease, Dyslipidemia) [3] and for 2,062 participants (Type 2 Diabetes Mellitus) [4]
 Criteria for evaluation are specificity and sensitivity of the tests as well as convenience, efficiency, and cost-effectiveness.[6].
 Comparative evaluation of TB diagnostic tests such as the gold standard Lowenstein-Jensen Method, the automated MB
Bac/t system, and the MODS Assay for the determination of TB and MDR-TB positive among sputum samples from the PGH
and the NTRL Laboratories [6].
INDUSTRIAL
BIOTECHNOLOGY
INDUSTRIAL BIOTECH
Globally Competitive and
Product and process technologies, Innovative Industry and
1.From Healthy sugars (xylitol from the hydrolysis products of corn cobs, corn fibers & sugarcane bagasse) Biotechnology Services
(Proposal) [41]
2. From Microbial-Based Colorants and Flavorings (Feb 28, 2015) [29]
Upscale Production using conventional fermentation runs of Industrial Enzymes (Proposal) [35]
Commercial products of Industrial Enzymes (Feb 9, 2015) [30]  Small-scale genetically engineered
Identified strains, cultures, applict’ns on Inoculants for fermentation in cacao beans (Dec 31, 2014) [22] 
microbial production of lactic acid
Preliminary Extraction & Application Studies of Industrial Enzymes (Jan 31, 2014) [23] Production / manufacturing of food
Upscale Production of Industrial Enzymes (Feb 28, 2014) [28], (Feb 9, 2015) [30] ingredients, enzymes, microbial
Microbial cultures and Product Application for Functional Food Ingredients (Aug 31, 2013) [20]. starter culture, and natural
Commercial products products00
1. From plant-growth hormone producers/biofertilizers (Aug 31, 2014) [24], (June 30, 2014) [25]
(June 30, 2014) [26].
2. Of Innoulant for Fermentation Process in cacao beans (Dec 31, 2014) [22]
3. Of Industrial Enzymes (Feb 28, 2014) [28],
Product & processing technology: Product Testing, Product development, Application studies,
1. For Plant Growth Hormone Producers/ Biofertilizers (Aug 31, 2014) [24], testing for Microbial-based Colorants & Flavoring
(June 30, 2014) [25] (June 30, 2014) [26]. (Monascus purpureus M108) [23]
2. And new packaging for fermentation process inoculant in cacao beans (Dec Extraction studies, varietal studies, product development &
31, 2014) [2] process design for Protein-Rich-by-Products feeds from
3. For Protein-Rich-by-Products feeds from Enzyme Processing (Feb 28, Enzyme Processing [28] [30].
2014) [28].
4. For Colorants and Flavorings (Feb 28, 2015) [29] Screening & other varietal studies, biochemical studies for :
5. For Aquaculture Probiotic f eeds(June 30, 2014) [27]. 1. Plant Growth Hormone Producers/ Biofertilizers [24], [25], [26]
6. From Pili pulp oil and by-product feed (June 30, 2014) [40]. 2. Aquaculture Probiotic feeds [27].
3. Protein-Rich-by-Products feeds from Enzyme Processing [28] [30].
 Identified strains, stable starter, cultures, targeted applications on Extraction studies, varietal studies, product dev’t & process design for
Inoculants for fermentation in cacao beans (Dec 31, 2014) [22] 1. Aquaculture Probiotic feeds [27].
 Product & processing technology 2. Plant Growth Hormone Producers/ Biofertilizers [24], [25], [26]
 Screening of strains, Optimization/Scale-up, Product Testing, Product Dev’t, Application
 Microbial cultures and Product Application for: 
studies, testing for Colorants & Flavoring &n Functional Food Ingredients [19], [20]
Screening, improvement of strains, prod’n process for fermentation in cacao beans [21]
a. Functional Food Ingredients (Aug 31, 2013) [20].
b. Colorants & Flavoring (Mar 31, 2013) [19].  Screening of isolates, process dev’t , applicat’n studies for fermentat’n in cacao beans [21]
 Scaling up, feasibility, setting up of incubation facility (5,000L) of industrial Enzymes [23]
 Identified strains, stable starter, cultures,  Optimization at lab scale, stability and quality testing of Industrial Enzymes [23]
applications for Indigenous Food
Fermentation(April 31, 2013) [21]
Establishment and optimization of the production of oil and protein enriched residues from pili pulp,
process testing and evaluation of product quality [40].
INDUSTRIAL BIOTECH:
Continuation… Globally Competitive and
Innovative Industry and
Biotechnology Services

Upscale processing technology of pili resin (Proposal) [42]

Product Safety Sheets, Stable Products, Shelf Life Information (Feb 28, 2015) [29] , (Proposals) [31] [32] [33]

Product and Lab scale processing technology of pili resin (Proposal) [42]
Large Scale Production using vectors for cry proteins as fertilizers (Proposal) [43]
Formulated Bacteriocin Product, Optimized Process (Proposal) [34] Pilot-scale genetically engineered
Lab Scale Production Technology of Other Industrial Enzymes (Proposal) [37] microbial production of ethanol &
Product and process technologies from Enzymes from Yacon(Proposal) [37] lactic acid for industrial consumption.
Commercial products pf Industrial Enzymes for Food and Beverage Applications (Proposal) [35]

Large Scale Production using vectors for cry proteins as fertilizers (Proposal) [43]
Lab Scale Production Technology of Other Industrial Enzymes (Proposal) [37]
Product and Lab scale processing technology of pili resin (Proposal) [42]
Formulated Bacteriocins Product, Optimized Process (Proposal) [34]
Product and process technologies Review and updating
1. From Healthy sugars (xylitol from the hydrolysis products Stabilization studies, packaging
of corn cobs, corn fibers and sugarcane bagasse) (Proposal) Advanced delivery studies
[41] Field testing, market testing, commercialization
2. From Microbial-Based Colorants and Flavorings
(Proposal) [31] [32] [33]
3. From Enzymes from Yacon(Proposal) [37]
Stable Strains, Patent Organisms, customized staeter cultures,
optimized process for starters of Indigenous Food Fermentation
(Proposal) [36]
Upscale Production using conventional fermentation runs of
Industrial Enzymes (Proposal) [35]