Sppo

INVESTIGATORY PROJECT WORK
Submitted By
Komal
Class: XII Science
Under the Guidance of

Miss Kulbeer Kour
PGT (Chemistry)
Department of Chemistry
Satguru Partap Singh International
school.
CERTIFICATE
This is to certify that Pushpa Class XII Science has prepared the
investigatory chemistry project entitled “To Study the digestion
of starch by salivary amylase and effect of temperature and pH
on it”. The report is the result of his efforts & endeavors.
The report is found worthy of acceptance as final project report
for the subject Chemistry of Class XII. He has prepared the report
under my guidance.
(Miss Kulbeer Kour)

PGT (Chemistry)
Department of Chemistry
Satguru Partap Singh International
school.
CERTIFICATE
The investigatory project entitled

“To Study the digestion of starch by salivary amylase and
effect of temperature and pH on it”
Submitted by Pushpa Class XII Science for the C.B.S.E

Senior Secondary Examination class XII of Chemistry
Practical at Satguru Partap Singh International school
has been examined.
SIGNATURE OF EXAMINER
DECLARATION
I hereby declare that the project work entitled

“To Study the digestion of starch by salivary
amylase and effect of temperature and pH on
it”, submitted to Department of Chemistry,
Kendriya Vidyalaya Khagaria is prepared by
me.
SHAHBAN ALI
Class XII Science

ACKNOWLEDGEMENT
I would like to express a deep sense of thanks & gratitude to my

project guide Mr. S.P. Gupta Sir for guiding me immensely through the
course of the project. He always evinced keen interest in my work. His
constructive advice & constant motivation have been responsible for the
successful completion of this project.
My sincere thanks go to Shri Yeshdeep Rohilla, Our principal sir,

for his co-ordination in extending every possible support for the
completion of this project.
I also thanks to my parents for their motivation & support. I must

thanks to my classmates for their timely help & support for compilation
of this project.
Last but not the least; I would like to thank all those who had
Helped directly or indirectly towards the completion of this project.
SHAHBAN ALI
Class: XII Science
CONTENTS
1. Certificate by Teacher. . . . . . . . . . . . . . .
2. Certificate by Examiner. . . . . . . . . . . . . .
3. Declaration. . . . . . . . . . . . . . . . . . . . . . . .
4. Acknowledgement. .. . . . . . . . . . . . . . . . .
5. Verification. . . . . . . . . . . . . . . . . . . . . . . .
6. Objectives of the Project. . . . . . . . . . . . .
7. Introduction. . . . . . . . . . . . . . . . . . . . . . .
8. Material Required . . . . . . . . . . . . . . . . . .
9. Procedure. . . . . . . . . . . . . . . . . . . . . . . . .
10. Observation . . . . . . . . . . . . . . . . . . . . . . .
11. CONCLUSION. . . . . . . . . . . . . . . . . . . . . .
12. BIBLIOGRAPHY. . . . . . . . . . . . . . . . . . . . .
Objectives of the Project Report
The main objective of this chemistry project

report is “To Study the digestion of starch by
salivary amylase and effect of temperature
and pH on it” and
1) To study digestion of starch by saliva.
2) To study the effect of temperature on the digestion of
starch by saliva.
3) To study the effect of pH on the salivary digestion of
starch.
Introduction
Every Health book insists on the chewing of food. The

act of chewing stimulates the excretion of saliva. Saliva
mixes up with the food and helps its digestion. That is,
the enzyme ptyalin or amylase present in human saliva
hydrolyzes the big molecules of food into many
molecules. For example starch into mono-saccnaricies
maltose and glucose; proteins into amino acids and fats
into fatty acids and glycerol. Thus saliva not only helps
in digestion of food but convert it into energy
generating substances. Further, enzymes and their
activity are very sensitive to temperature and ph. Even
a slight variation in these two factors, can disturb the
action of enzymes. In other words, digestion of food by
salivary amylase is also effected by pH and temperature
and can be verified experimentally. For example,
hydrolysis of starch can be verified by testing it with
iodine solution. Starch forms blue coloured complex
with iodine. If no starch is present in a system it will not
give blue colour with iodine.
Material Required
The requirements for experiment of Chemistry

Project Report are as under:
1) Test tubes
2) Test tube stand
3) One dropper
4) Beaker
5) Stop watch
6) Starch and Iodine solution
7) Thermometer
8) Dilute HCL and Dilute NaOH solution.

Chemistry Experiment 1 Procedure
Procedure for Chemistry Experiment 1 is:
1) Collection of Saliva – Rinse mouth throughly with cold

water and ensure that it does not contain any food
particles. Now take about 20ml of lupe warm water in
the mouth and gangle for about three minutes so that
saliva mixes up well with it. Spit this into a beaker.
Filter, if there is any suspended impurity clear filtrate is
saliva solution and contains enzyme ptyalin.
2) Preparation of starch solution – Take about 0.5g of
starch in 100ml beaker and add enough water to make a
paste. Dilute the paste by adding 50ml water and boil for
about 5 minutes.
3) Digestion of starch :-
(a) Take 5ml of the starch solution in a test tube. Add
2ml of saliva solution into it. Mix the solutions well
by shaking the tube carefully and start a step watch.
(b) After one minute take out two drops of the mixture
solution from the test tube with the help of a dropper
and transfer it into another test tube containing about
one 1ml of 1% iodine solution. Note the colour
produced, if any.
(c) Repeat this test after every one minute taking two
drops of the mixture solution and fresh 1% iodine
solution continue until the test shows no blue colour.
Record the time and blue colour intensity.
Observation
Time Passed 1 2 3 4
after Mixing Minute Minute Minute Minute
Colour Deep Blue Light No Blue
Intensity Blue Blue
Absence of blue colour on addition to iodine solution

means absence of starch in the mixture solution. That is
whole of the starch has got digested or hydrolysed.
Chemistry Experiment 2
Procedure: - Effect of temperature on the digestion of starch

by saliva.
1) Take three test tubes and label these 1, 2, and 3.

2) Take 5ml of the starch solution, 2ml of the saliva solution
and 5ml of water in each test tube.
3) Place test tube Number 1 in water at room temperature, test
tube Number 2 in a beaker containing water at 500 C and
test tube Number 3 in boiling water.
4) After 5 minutes, observe the colour change on mixing two
drops of the mixture of every tube with one ml of 1%
iodine solution. Note the intensity of blue coloured form.
Conclusion
Starch get hydrolysed by saliva amylase

Chemistry Experiment 3
Procedure: - To study the effect of pH on the salivary

digestion of starch
1) Take three test tubes and label these 1, 2, and 3.

2) Add 5ml of the starch solution, 2ml of the saliva
solution in each test tube.
3) Now ass 2ml of water in test tube Number 1 2ml of
Dilute HCL in test tube Number 2 and 2ml of Dilute
NaOH solution in test tube Number 3 and shake
carefully.
4) Keep the three test tubes in water at room temperature
for about 10 minutes.
5) Add 2 drops of the solution of each test tube with 1%
iodine solution and observe the colour change
Conclusion
Temperature effects the digestion of starch by salivary

with increase in temperature salivary amylase get
inactivated and process of digestion do not take place.
BIBLIOGRAPHY
1 http://www.google.com/
2 http://en.wikipedia.org
3 N.C.E.R.T Class 12 Chemistry
4 Dinesh Chemistry
5 Comprehensive Chemistry

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INVESTIGATORY PROJECT WORK

Under the Guidance of

(Miss Kulbeer Kour)

The investigatory project entitled

Submitted by Pushpa Class XII Science for the C.B.S.E

I hereby declare that the project work entitled

Class XII Science

I would like to express a deep sense of thanks & gratitude to my

My sincere thanks go to Shri Yeshdeep Rohilla, Our principal sir,

I also thanks to my parents for their motivation & support. I must

6. Objectives of the Project. . . . . . . . . . . . .

The main objective of this chemistry project

Every Health book insists on the chewing of food. The

The requirements for experiment of Chemistry

2) Test tube stand

6) Starch and Iodine solution

8) Dilute HCL and Dilute NaOH solution.

Procedure for Chemistry Experiment 1 is:

1) Collection of Saliva – Rinse mouth throughly with cold

Absence of blue colour on addition to iodine solution

Procedure: - Effect of temperature on the digestion of starch

1) Take three test tubes and label these 1, 2, and 3.

Starch get hydrolysed by saliva amylase

Procedure: - To study the effect of pH on the salivary

1) Take three test tubes and label these 1, 2, and 3.

Temperature effects the digestion of starch by salivary

3 N.C.E.R.T Class 12 Chemistry

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