Nothing Special   »   [go: up one dir, main page]

You seem to have javascript disabled. Please note that many of the page functionalities won't work as expected without javascript enabled.
 
 
Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (129)

Search Parameters:
Keywords = gray projection

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
16 pages, 6458 KiB  
Article
Modeling the Effects of Extreme Temperatures on the Infection Rate of Botrytis cinerea Using Historical Climate Data (1951–2023) of Central Chile
by William Campillay-Llanos, Samuel Ortega-Farías, Patricio González-Colville, Gonzalo A. Díaz, Marlon M. López-Flores and Rafael López-Olivari
Agronomy 2025, 15(3), 608; https://doi.org/10.3390/agronomy15030608 - 28 Feb 2025
Viewed by 219
Abstract
Extreme maximum temperatures in summer present a significant risk to agroindustry as crops and their ecological interactions have critical thermal limits that can affect their performance and microorganisms-related. Gray mold disease caused by Botrytis cinerea is the most critical disease affecting crops worldwide. [...] Read more.
Extreme maximum temperatures in summer present a significant risk to agroindustry as crops and their ecological interactions have critical thermal limits that can affect their performance and microorganisms-related. Gray mold disease caused by Botrytis cinerea is the most critical disease affecting crops worldwide. In this sense, the impact of temperature on agricultural productivity is well documented in the Northern Hemisphere; the risk of extreme temperatures on the infection rate of B. cinerea in Central Chile is limited. This study analyzes historical climate data from January and February between 1951 and 2023 for the cities of Santiago, Talca, Chillán, and Los Ángeles. The aim was to examine trends in extreme maximum temperatures (EMTs) and develop a simple model to estimate the infection rate of B. cinerea. Linear trend analyses were conducted, as was analysis of the probability of occurrence. Additionally, five-year averages were calculated, and a generic model was presented to assess the effects of warming on the infection rate. The analysis shows positive growth in extreme maximum temperatures in January and February, with projections for 2024, 2025, and 2026 at 70%, 80%, and 80%, respectively. February showed the most significant thermal increase among all stations, with Chillán and Los Ángeles recording higher increases than Santiago and Talca. Projections suggest temperatures near 40–41 °C. The five-year averages for Chillán and Los Ángeles exceeded 37 °C in the 2016–2020 period, the highest values during the analyzed time frame. Trends for 2021–2026 indicate upper limits above 38 °C. These trends, combined with dry summers, could increase the severity of infections and modify the optimal thermal conditions for the pathogen. The results suggest that thermal changes could reduce the infection risk by B. cinerea on fruit crops in Central Chile, and a theoretical approach is proposed to develop predictive tools to facilitate risk assessment in a warming environment. Full article
(This article belongs to the Section Precision and Digital Agriculture)
Show Figures

Figure 1

Figure 1
<p>Location of study sites in the Central Valley, comprising Santiago, Talca, Chillán, and Los Ángeles, representing the agricultural region of mainland Central Chile, South America.</p>
Full article ">Figure 2
<p>Conceptual diagram illustrating the Climate Trends Equation, which includes the relationship between EMT (Y), year (X), the growth rate (a), and the intercept (b). Polygons using standard deviation (σ) are represented to demonstrate the fitting process.</p>
Full article ">Figure 3
<p>Temperature-dependent risk model (r(T)) for <span class="html-italic">Botrytis cinerea</span> infection. The study integrates a temperature-dependent risk model (Equation (2)) incorporating Tmax, Tmin, and Top representing maximum, minimum, and optimum temperatures. σ is the standard deviation for overestimation and underestimation scenarios. A threshold value 0.5 for r(T) was considered significant, providing insights into <span class="html-italic">B. cinerea</span> infection dynamics under varying temperature conditions.</p>
Full article ">Figure 4
<p>Temporal analysis of extreme maximum temperature (EMT) trends in Santiago, Central Chile. Panels (<b>a</b>,<b>b</b>) represent data for January, with (<b>a</b>) showing trends during the 20th century and (<b>b</b>) depicting trends in the early 21st century. Similarly, panels (<b>c</b>,<b>d</b>) correspond to February, with (<b>c</b>) presenting 20th-century data and (<b>d</b>) showing early 21st-century trends.</p>
Full article ">Figure 5
<p>Temporal analysis of extreme maximum temperature (EMT) trends in Talca, Central Chile. Panels (<b>a</b>,<b>b</b>) represent data for January, with (<b>a</b>) showing trends during the 20th century and (<b>b</b>) depicting trends in the early 21st century. Similarly, panels (<b>c</b>,<b>d</b>) correspond to February, with (<b>c</b>) presenting 20th-century data and (<b>d</b>) showing early 21st-century trends.</p>
Full article ">Figure 6
<p>Temporal analysis of extreme maximum temperature (EMT) trends in Chillán, Central Chile. Panels (<b>a</b>,<b>b</b>) represent data for January, with (<b>a</b>) showing trends during the 20th century and (<b>b</b>) depicting trends in the early 21st century. Similarly, panels (<b>c</b>,<b>d</b>) correspond to February, with (<b>c</b>) presenting 20th-century data and (<b>d</b>) showing early 21st-century trends.</p>
Full article ">Figure 7
<p>Temporal analysis of extreme maximum temperature (EMT) trends in Los Ángeles, Central Chile. Panels (<b>a</b>,<b>b</b>) represent data for January, with (<b>a</b>) showing trends during the 20th century and (<b>b</b>) depicting trends in the early 21st century. Similarly, panels (<b>c</b>,<b>d</b>) correspond to February, with (<b>c</b>) presenting 20th-century data and (<b>d</b>) showing early 21st-century trends.</p>
Full article ">Figure 8
<p>Average February temperatures in Santiago from 1921 to 2023. The blue bars represent the temperatures for each five-year period, while the yellow bar corresponding to the 2021–2023 period indicates that the full 5 years are not completed for this time range.</p>
Full article ">Figure 9
<p>Average February temperatures in Talca from 1921 to 2023. The blue bars represent the temperatures for each five-year period, while the yellow bar corresponding to the 2021–2023 period indicates that the full 5 years are not completed for this time range.</p>
Full article ">Figure 10
<p>Average February temperatures in Chillán from 1951 to 2023. The blue bars represent the temperatures for each five-year period, while the yellow bar corresponding to the 2021–2023 period indicates that the full 5 years are not completed for this time range.</p>
Full article ">Figure 11
<p>Average February temperatures in Los Ángeles from 1951 to 2023. The blue bars represent the temperatures for each five-year period, while the yellow bar corresponding to the 2021–2023 period indicates that the full 5 years are not completed for this time range.</p>
Full article ">Figure 12
<p>Temperature-dependent infection risk, r(T), for <span class="html-italic">Botrytis cinerea</span> pathogen in four cities in Central Chile: Santiago, Talca, Chillán, and Los Ángeles. Recorded data, underestimation, and overestimation scenarios are depicted.</p>
Full article ">
21 pages, 14246 KiB  
Article
Three-Dimensional Multi-Material Topology Optimization: Applying a New Mapping-Based Projection Function
by Hélio Luiz Simonetti, Francisco de Assis das Neves, Valério Silva Almeida, Marcio Maciel da Silva and Luttgardes de Oliveira Neto
Materials 2025, 18(5), 997; https://doi.org/10.3390/ma18050997 - 24 Feb 2025
Viewed by 180
Abstract
This paper presents an efficient and compact MATLAB code for 3D topology optimization of multi-materials. The multi-material problem using a mapping-based material interpolation function is adopted from previous work, in which each material is modeled in the same way, presenting a clear (clean) [...] Read more.
This paper presents an efficient and compact MATLAB code for 3D topology optimization of multi-materials. The multi-material problem using a mapping-based material interpolation function is adopted from previous work, in which each material is modeled in the same way, presenting a clear (clean) result of 0 and 1 for each material of the optimized structures, without gray elements, thus facilitating the manufacturing process. A new projection function, the sigmoid function, is adopted for the filtered design variables for each material in the domain. The proposed method improves computational efficiency, reducing computational costs by up to 36.7%, while achieving a 19.1% improvement in the objective function compared to the hyperbolic tangent function. A multi-material topology optimization solution with minimal compliance under volume constraints, including details of the optimization model, filtering, projection, and sensitivity analysis procedures, is presented. Numerical examples are also used to demonstrate the effectiveness of the code, and the influence of the position of the support on the optimized results is also proven. The complete MATLAB code for 3D elastic structures is presented as an example. Full article
Show Figures

Figure 1

Figure 1
<p>Projection function: (<b>a</b>) hyperbolic tangent function; (<b>b</b>) sigmoid function.</p>
Full article ">Figure 2
<p>Design domain and boundary conditions.</p>
Full article ">Figure 3
<p>Optimal topologies for three materials: (<b>a</b>) Topology using sigmoid function approach of this article; (<b>b</b>) Topology using hyperbolic tangent function proposed by [<a href="#B32-materials-18-00997" class="html-bibr">32</a>].</p>
Full article ">Figure 4
<p>Design domain and boundary conditions.</p>
Full article ">Figure 5
<p>Optimal topologies for three materials: (<b>a</b>) Topology using sigmoid function approach of this article; (<b>b</b>) Topology using hyperbolic tangent function proposed by [<a href="#B32-materials-18-00997" class="html-bibr">32</a>].</p>
Full article ">Figure 6
<p>Design domain and boundary condition: (<b>a</b>) bridge with end supports (<b>b</b>) bridge with recessed supports.</p>
Full article ">Figure 7
<p>Optimal topologies for three materials with solutions via PCG: supports at the ends—(<b>a</b>) Topology using hyperbolic tangent function proposed by [<a href="#B32-materials-18-00997" class="html-bibr">32</a>] and (<b>b</b>) Topology using sigmoid function approach of this article.</p>
Full article ">Figure 8
<p>Optimal topologies for three materials with solutions via incomplete Cholesky: bridge with ends supports—(<b>a</b>) Topology using hyperbolic tangent function proposed by [<a href="#B32-materials-18-00997" class="html-bibr">32</a>] and (<b>b</b>) Topology using sigmoid function approach of this article.</p>
Full article ">Figure 9
<p>Optimal topologies for three materials with solutions via PCG: with recessed support—(<b>a</b>) Topology using hyperbolic tangent function proposed by [<a href="#B32-materials-18-00997" class="html-bibr">32</a>] and (<b>b</b>) Topology using sigmoid function approach of this article.</p>
Full article ">Figure 10
<p>Optimal topologies for three materials with solutions via incomplete Cholesky: with recessed support—(<b>a</b>) Topology using hyperbolic tangent function proposed by [<a href="#B32-materials-18-00997" class="html-bibr">32</a>] and (<b>b</b>) Topology using sigmoid function approach of this article.</p>
Full article ">Figure 11
<p>Design domain and boundary conditions.</p>
Full article ">Figure 12
<p>Optimal topologies using 10 materials in increasing order of E: (<b>a</b>) Lateral view and (<b>b</b>) Oblique view.</p>
Full article ">Figure 13
<p>Optimal topologies using 10 materials in decreasing order of E: (<b>a</b>) Lateral view and (<b>b</b>) Oblique view.</p>
Full article ">Figure 14
<p>Graph of objective function, volume, and beta by iteration.</p>
Full article ">
14 pages, 4564 KiB  
Article
Exploring Climate and Air Pollution Mitigating Benefits of Urban Parks in Sao Paulo Through a Pollution Sensor Network
by Patrick Connerton, Thiago Nogueira, Prashant Kumar, Maria de Fatima Andrade and Helena Ribeiro
Int. J. Environ. Res. Public Health 2025, 22(2), 306; https://doi.org/10.3390/ijerph22020306 - 18 Feb 2025
Viewed by 254
Abstract
Ambient air pollution is the most important environmental factor impacting human health. Urban landscapes present unique air quality challenges, which are compounded by climate change adaptation challenges, as air pollutants can also be affected by the urban heat island effect, amplifying the deleterious [...] Read more.
Ambient air pollution is the most important environmental factor impacting human health. Urban landscapes present unique air quality challenges, which are compounded by climate change adaptation challenges, as air pollutants can also be affected by the urban heat island effect, amplifying the deleterious effects on health. Nature-based solutions have shown potential for alleviating environmental stressors, including air pollution and heat wave abatement. However, such solutions must be designed in order to maximize mitigation and not inadvertently increase pollutant exposure. This study aims to demonstrate potential applications of nature-based solutions in urban environments for climate stressors and air pollution mitigation by analyzing two distinct scenarios with and without green infrastructure. Utilizing low-cost sensors, we examine the relationship between green infrastructure and a series of environmental parameters. While previous studies have investigated green infrastructure and air quality mitigation, our study employs low-cost sensors in tropical urban environments. Through this novel approach, we are able to obtain highly localized data that demonstrates this mitigating relationship. In this study, as a part of the NERC-FAPESP-funded GreenCities project, four low-cost sensors were validated through laboratory testing and then deployed in two locations in São Paulo, Brazil: one large, heavily forested park (CIENTEC) and one small park surrounded by densely built areas (FSP). At each site, one sensor was located in a vegetated area (Park sensor) and one near the roadside (Road sensor). The locations selected allow for a comparison of built versus green and blue areas. Lidar data were used to characterize the profile of each site based on surrounding vegetation and building area. Distance and class of the closest roadways were also measured for each sensor location. These profiles are analyzed against the data obtained through the low-cost sensors, considering both meteorological (temperature, humidity and pressure) and particulate matter (PM1, PM2.5 and PM10) parameters. Particulate matter concentrations were lower for the sensors located within the forest site. At both sites, the road sensors showed higher concentrations during the daytime period. These results further reinforce the capabilities of green–blue–gray infrastructure (GBGI) tools to reduce exposure to air pollution and climate stressors, while also showing the importance of their design to ensure maximum benefits. The findings can inform decision-makers in designing more resilient cities, especially in low-and middle-income settings. Full article
Show Figures

Figure 1

Figure 1
<p>Study site location within Brazil (<b>A</b>) and presence of roadways, vegetation and built area at the CIENTEC (<b>B</b>,<b>C</b>) and FSP (<b>D</b>,<b>E</b>) sites.</p>
Full article ">Figure 2
<p>Summary of characteristics of Park (green) and Roadside (brown) sensors for FSP (solid) and CIENTEC (gridded) sites.</p>
Full article ">Figure 3
<p>Hourly variations in concentrations of PM<sub>1</sub> (<b>top</b>), PM <sub>2.5</sub> (<b>middle</b>) and PM<sub>10</sub> (<b>bottom</b>) for the FSP (<b>left</b>) and CIENTEC (<b>right</b>) sites.</p>
Full article ">Figure 4
<p>Top (<b>A</b>,<b>D</b>) and side (<b>B</b>,<b>E</b>) views of vegetation barriers at each site and point cloud density distribution (<b>C</b>,<b>F</b>) for FSP (<b>bottom</b>) and CIENTEC (<b>top</b>). The red circles indicate the sensor locations for the top view.</p>
Full article ">Figure 5
<p>Percentile rose distribution of the difference between road and park PM<sub>2.5</sub> concentrations at FSP (<b>left</b>) and CIENTEC (<b>right</b>) sites.</p>
Full article ">
26 pages, 1293 KiB  
Review
Moving on to Greener Pastures? A Review of South Africa’s Housing Megaproject Literature
by Louis Lategan, Brian Fisher-Holloway, Juanee Cilliers and Sarel Cilliers
Sustainability 2025, 17(4), 1677; https://doi.org/10.3390/su17041677 - 18 Feb 2025
Viewed by 286
Abstract
South Africa is a leader in the scholarship on green urbanism in the Global South, but academic progress has not translated to broad implementation. Notably, government-subsidized housing projects have produced peripheral developments featuring low build quality, conventional gray infrastructure, and deficient socio-economic and [...] Read more.
South Africa is a leader in the scholarship on green urbanism in the Global South, but academic progress has not translated to broad implementation. Notably, government-subsidized housing projects have produced peripheral developments featuring low build quality, conventional gray infrastructure, and deficient socio-economic and environmental amenities. Declining delivery and increasing informal settlement spawned a 2014 shift to housing megaprojects to increase output and improve living conditions, socio-economic integration, and sustainability. The shift offered opportunities for a normative focus on greener development mirrored in the discourse surrounding project descriptions. Yet, the level of enactment has remained unclear. In reflecting on these points, this paper employs environmental justice as a theoretical framework and completes a comprehensive review of the academic literature on housing megaprojects and the depth of their greener development commitments. A three-phase, seven-stage review protocol retrieves the relevant literature, and bibliometric and qualitative content analyses identify publication trends and themes. Results indicate limited scholarship on new megaprojects with sporadic and superficial references to greener development, mostly reserved for higher-income segments and private developments. In response, this paper calls for more determined action to launch context-aware and just greener megaprojects and offers corresponding guidance for research and practice of value to South Africa and beyond. Full article
Show Figures

Figure 1

Figure 1
<p>Environmental justice framework for South African housing megaprojects.</p>
Full article ">Figure 2
<p>Review approach followed.</p>
Full article ">
25 pages, 11268 KiB  
Article
Optimized Random Forest Models for Rock Mass Classification in Tunnel Construction
by Bo Yang, Danial Jahed Armaghani, Hadi Fattahi, Mohammad Afrazi, Mohammadreza Koopialipoor, Panagiotis G. Asteris and Manoj Khandelwal
Geosciences 2025, 15(2), 47; https://doi.org/10.3390/geosciences15020047 - 2 Feb 2025
Viewed by 615
Abstract
The accurate prediction of rock mass quality ahead of the tunnel face is crucial for optimizing tunnel construction strategies, enhancing safety, and reducing geological risks. This study developed three hybrid models using random forest (RF) optimized by moth-flame optimization (MFO), gray wolf optimizer [...] Read more.
The accurate prediction of rock mass quality ahead of the tunnel face is crucial for optimizing tunnel construction strategies, enhancing safety, and reducing geological risks. This study developed three hybrid models using random forest (RF) optimized by moth-flame optimization (MFO), gray wolf optimizer (GWO), and Bayesian optimization (BO) algorithms to classify the surrounding rock in real time during tunnel boring machine (TBM) operations. A dataset with 544 TBM tunneling samples included key parameters such as thrust force per cutter (TFC), revolutions per minute (RPM), penetration rate (PR), advance rate (AR), penetration per revolution (PRev), and field penetration index (FPI), with rock classification based on the Rock Mass Rating (RMR) method. To address the class imbalance, the Borderline Synthetic Minority Over-Sampling Technique was applied. Performance assessments revealed the MFO-RF model’s superior performance, with training and testing accuracies of 0.992 and 0.927, respectively, and key predictors identified as PR, AR, and RPM. Additional validation using 91 data sets confirmed the reliability of the MFO-RF model on unseen data, achieving an accuracy of 0.879. A graphical user interface was also developed, enabling field engineers and technicians to make instant and reliable rock classification predictions, greatly supporting safe tunnel construction and operational efficiency. These models contribute valuable tools for real-time, data-driven decision-making in tunneling projects. Full article
(This article belongs to the Special Issue Fracture Geomechanics—Obstacles and New Perspectives)
Show Figures

Figure 1

Figure 1
<p>Framework of RF for solving classification problems.</p>
Full article ">Figure 2
<p>The orientation behavior of moths: (<b>a</b>) moths maintain a constant flight angle relative to the moon; (<b>b</b>) moths spiral towards an artificial light source.</p>
Full article ">Figure 3
<p>Grey Wolf Hierarchy.</p>
Full article ">Figure 4
<p>The overall construction process of the hybrid models.</p>
Full article ">Figure 5
<p>Percentage distribution of different rock grades in the dataset.</p>
Full article ">Figure 6
<p>Correlation matrix depicting relationships among variables in the TBM database.</p>
Full article ">Figure 7
<p>Box plots presenting statistical metrics for six variables.</p>
Full article ">Figure 8
<p>Schematic diagram illustrating the calculation of evaluation indices.</p>
Full article ">Figure 9
<p>Iterative convergence graphs of three hybrid models.</p>
Full article ">Figure 9 Cont.
<p>Iterative convergence graphs of three hybrid models.</p>
Full article ">Figure 10
<p>Confusion matrix for each model in the training stage.</p>
Full article ">Figure 11
<p>The final ranks of models during the training stage.</p>
Full article ">Figure 12
<p>Confusion matrix for each model in the testing stage.</p>
Full article ">Figure 13
<p>The final ranks of models during the testing stage.</p>
Full article ">Figure 14
<p>The performance comparison of MFO-RF and other ML models.</p>
Full article ">Figure 15
<p>SHAP to interpret MFO-RF for the prediction of rock mass classification with three categories.</p>
Full article ">Figure 15 Cont.
<p>SHAP to interpret MFO-RF for the prediction of rock mass classification with three categories.</p>
Full article ">Figure 16
<p>The relative importance of the total variables of the three classes of the surrounding rock.</p>
Full article ">Figure 17
<p>Hybrid model performances on the validation dataset.</p>
Full article ">Figure 18
<p>GUI for predicting rock mass classification.</p>
Full article ">
32 pages, 8520 KiB  
Article
Spatial-Temporal Variation and Driving Forces of Carbon Storage at the County Scale in China Based on a Gray Multi-Objective Optimization-Patch-Level Land Use Simulation-Integrated Valuation of Ecosystem Services and Tradeoffs-Optimal Parameter-Based Geographical Detector Model: Taking the Daiyun Mountain’s Rim as an Example
by Gui Chen, Qingxia Peng, Qiaohong Fan, Wenxiong Lin and Kai Su
Land 2025, 14(1), 14; https://doi.org/10.3390/land14010014 - 25 Dec 2024
Viewed by 591
Abstract
Exploring and predicting the spatiotemporal evolution characteristics and driving forces of carbon storage in typical mountain forest ecosystems under land-use changes is crucial for curbing the effects of climate change and fostering sustainable, eco-friendly growth. The existing literature provides important references for our [...] Read more.
Exploring and predicting the spatiotemporal evolution characteristics and driving forces of carbon storage in typical mountain forest ecosystems under land-use changes is crucial for curbing the effects of climate change and fostering sustainable, eco-friendly growth. The existing literature provides important references for our related studies but further expansion and improvements are needed in some aspects. This study first proposed an integrated framework comprising gray multi-objective optimization (GMOP), Integrated Valuation of Ecosystem Services and Tradeoffs (InVEST), the Patch-level Land Use Simulation Model (PLUS), and optimal parameter-based geographical detector (OPGD) models to further expand and improve on existing research. Then, the integrated model was used to analyze the spatial–temporal variation in land-use pattern and carbon storage at the county scale in China’s Daiyun Mountain’s Rim under four scenarios in 2032, and analyze the driving force of spatial differentiation of carbon storage. The results indicated that (1) land-use change primarily involves the mutual transfer among forest, cultivated, and construction land, with approximately 7.2% of the land-use type area undergoing a transition; (2) in 2032, the natural development scenario projects a significant reduction in forest land and an expansion of cultivated, shrub, and construction lands. Conversely, the economic priority, ecological priority, and economic–ecological coordinated scenarios all anticipate a decline in cultivated land area; (3) in 2032, the natural development scenario will see a 2.8 Tg drop in carbon stock compared to 2022. In contrast, the economic priority, ecological priority, and economic–ecological coordinated scenarios are expected to increase carbon storage by 0.29 Tg, 2.62 Tg, and 1.65 Tg, respectively; (4) the spatial differentiation of carbon storage is jointly influenced by various factors, with the annual mean temperature, night light index, elevation, slope, and population density being the key influencing factors. In addition, the influence of natural factors on carbon storage is diminishing, whereas the impact of socioeconomic factors is on the rise. This study deepened, to a certain extent, the research on spatiotemporal dynamics simulation of carbon storage and its driving mechanisms under land-use changes in mountainous forest ecosystems. The results can serve to provide scientific support for carbon balance management and climate adaptation strategies at the county scale while also offering case studies that can inform similar regions around the world. However, several limitations remain, as follows: the singularity of carbon density data, and the research scope being confined to small-scale mountainous forest ecosystems. Future studies could consider collecting continuous annual soil carbon density data and employing land-use simulation models (such as PLUS or CLUMondo) appropriate to the study area’s dimensions. Full article
Show Figures

Figure 1

Figure 1
<p>Overview of the Daiyun Mountain’s Rim.</p>
Full article ">Figure 2
<p>Spatial distribution characteristics of land-use types in the Daiyun Mountain’s Rim from 1992 to 2032: (<b>a</b>–<b>g</b>) represent the spatial distribution of land-use types in 1992, 1997, 2002, 2007, 2012, 2017, and 2022 in the Daiyun Mountain’s Rim, respectively; (<b>h</b>–<b>k</b>) represent the 2032 nature development scenario, 2032 economic priority development scenario, 2032 ecological priority development scenario, and 2032 coordinated economic and ecological development scenario in the Daiyun Mountain’s Rim, respectively.</p>
Full article ">Figure 3
<p>The spatial distribution pattern and change trend of carbon storage in the Daiyun Mountain’s Rim from 1992 to 2032.</p>
Full article ">Figure 4
<p>Spatial changes in land use in the Daiyun Mountain’s Rim from 1992 to 2022.</p>
Full article ">Figure 5
<p>Changes in land uses and carbon stocks in the Daiyun Mountain’s Rim from 1992 to 2022: (<b>a</b>) changes in land uses in the Daiyun Mountain’s Rim from 1992 to 2022; (<b>b</b>) changes in carbon stocks in the Daiyun Mountain’s Rim from 1992 to 2022.</p>
Full article ">Figure 6
<p>Impacts of major land type shifts on carbon stocks in different counties of the Daiyun Mountain’s Rim.</p>
Full article ">Figure 7
<p>Distributional differentiation of carbon stocks in multi-dimensional topographic environments: (<b>a</b>) carbon storage changes at different elevations; (<b>b</b>) carbon storage changes across slope gradients; (<b>c</b>) carbon storage changes across topographic wetness index categories; (<b>d</b>) average carbon storage changes at different elevations; (<b>e</b>) average carbon storage changes across slope gradients; (<b>f</b>) average carbon storage changes across topographic wetness index categories.</p>
Full article ">Figure 8
<p>Results of the interactive detection of carbon stock drivers in the Daiyun Mountain’s Rim from 1992 to 2002: (<b>a</b>–<b>g</b>) represent the results of the interactive detection of carbon stock drivers in 1992, 1997, 2002, 2007, 2012, 2017, and 2022 in the Daiyun Mountain’s Rim, respectively, and the legend is below figure (<b>g</b>); (<b>h</b>) represent the value changes in the Daiyun Mountain’s Rim from 1992 to 2022, and the legend is below figure (<b>h</b>).</p>
Full article ">
16 pages, 4152 KiB  
Article
Computer Vision-Based Fire–Ice Ion Algorithm for Rapid and Nondestructive Authentication of Ziziphi Spinosae Semen and Its Counterfeits
by Peng Chen, Xutong Shao, Guangyu Wen, Yaowu Song, Rao Fu, Xiaoyan Xiao, Tulin Lu, Peina Zhou, Qiaosheng Guo, Hongzhuan Shi and Chenghao Fei
Foods 2025, 14(1), 5; https://doi.org/10.3390/foods14010005 - 24 Dec 2024
Viewed by 783
Abstract
The authentication of Ziziphi Spinosae Semen (ZSS), Ziziphi Mauritianae Semen (ZMS), and Hovenia Acerba Semen (HAS) has become challenging. The chromatic and textural properties of ZSS, ZMS, and HAS are analyzed in this study. Color features were extracted via RGB, CIELAB, and HSI [...] Read more.
The authentication of Ziziphi Spinosae Semen (ZSS), Ziziphi Mauritianae Semen (ZMS), and Hovenia Acerba Semen (HAS) has become challenging. The chromatic and textural properties of ZSS, ZMS, and HAS are analyzed in this study. Color features were extracted via RGB, CIELAB, and HSI spaces, whereas texture information was analyzed via the gray-level co-occurrence matrix (GLCM) and Law’s texture feature analysis. The results revealed significant differences in color and texture among the samples. The fire–ice ion dimensionality reduction algorithm effectively fuses these features, enhancing their differentiation ability. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) confirmed the algorithm’s effectiveness, with variable importance in projection analysis (VIP analysis) (VIP > 1, p < 0.05) highlighting significant differences, particularly for the fire value, which is a key factor. To further validate the reliability of the algorithm, Back Propagation Neural Network (BP), Support Vector Machine (SVM), Deep Belief Network (DBN), and Random Forest (RF) were used for reverse validation, and the accuracy of the training set and test set reached 98.83–100% and 95.89–99.32%, respectively. The method provides a simple, low-cost, and high-precision tool for the fast and nondestructive detection of food authenticity. Full article
Show Figures

Figure 1

Figure 1
<p>Sample information (<b>A</b>) and radar chart of colorimetric values (<b>B</b>) of ZSS, ZMS, and HAS. ZSS, Ziziphi Spinosae Semen; ZMS, Ziziphi Mauritianae Semen; HAS, Hovenia Acerba Semen.</p>
Full article ">Figure 2
<p>GLCM texture parameter histogram (<b>A</b>) and Law’s texture parameter heatmap (<b>B</b>) of ZSS, ZMS, and HAS. ZSS, Ziziphi Spinosae Semen; ZMS, Ziziphi Mauritianae Semen; HAS, Hovenia Acerba Semen.</p>
Full article ">Figure 3
<p>Fire–ice value box chart (<b>A</b>) and fire–ice chart (<b>B</b>) of ZSS, ZMS, and HAS. The letters (a–c) above the bars indicate significant differences as determined by Duncan’s multiple-range test (<span class="html-italic">p</span> &lt; 0.05). ZSS, Ziziphi Spinosae Semen; ZMS, Ziziphi Mauritianae Semen; HAS, Hovenia Acerba Semen.</p>
Full article ">Figure 4
<p>Score plots of the PCA model for ZSS, ZMS, and HAS of raw color and texture characterization (<b>A</b>); score plots of the PLS-DA model for ZSS, ZMS, and HAS of raw color and texture characterization (<b>B</b>). ZSS, Ziziphi Spinosae Semen; ZMS, Ziziphi Mauritianae Semen; HAS, Hovenia Acerba Semen. PCA, principal component analysis; PLS-DA, partial least squares discrimination analysis.</p>
Full article ">Figure 5
<p>Cross-validation results with 200 calculations using a permutation test for ZSS, ZMS, and HAS of raw color and texture characterization (<b>A</b>); VIP plots for ZSS, ZMS, and HAS of raw color and texture characterization (<b>B</b>). ZSS, Ziziphi Spinosae Semen; ZMS, Ziziphi Mauritianae Semen; HAS, Hovenia Acerba Semen. VIP, variable importance for projecting.</p>
Full article ">Figure 6
<p>Score plots of the PCA model for ZSS, ZMS, and HAS of fire–ice ions dimensionality reduction data (<b>A</b>); score plots of the PLS-DA model for ZSS, ZMS, and HAS of fire–ice ions dimensionality reduction data (<b>B</b>). ZSS, Ziziphi Spinosae Semen; ZMS, Ziziphi Mauritianae Semen; HAS, Hovenia Acerba Semen. PCA, principal component analysis; PLS-DA, partial least squares discrimination analysis.</p>
Full article ">Figure 7
<p>Cross-validation results with 200 calculations using a permutation test for ZSS, ZMS, and HAS of fire–ice ions dimensionality reduction data (<b>A</b>); VIP plots for ZSS, ZMS, and HAS of fire–ice ions dimensionality reduction data (<b>B</b>). ZSS, Ziziphi Spinosae Semen; ZMS, Ziziphi Mauritianae Semen; HAS, Hovenia Acerba Semen. VIP, variable importance for projecting.</p>
Full article ">Figure 8
<p>Evaluation metrics of 4 machine learning algorithms (BP, SVM, DBN, and RF).</p>
Full article ">
20 pages, 33934 KiB  
Article
Distribution of Bird Communities and Habitat Corridor Composition Shaped by Environmental Factors in Urbanized Landscapes: A Case Study in Beijing, China
by Lingqian Tan, Ruiqi Huang, Peiyao Hao, Zhipeng Huang and Yinglin Wang
Forests 2025, 16(1), 1; https://doi.org/10.3390/f16010001 - 24 Dec 2024
Viewed by 907
Abstract
Urban biodiversity is crucial for ecological security, balance, and important for fostering awareness on human-nature interconnectedness among the public. The diversity of birds, as an urban ecosystem indicator, reflects ecosystem services and is impacted by urban development. To explore the impacts of urbanization [...] Read more.
Urban biodiversity is crucial for ecological security, balance, and important for fostering awareness on human-nature interconnectedness among the public. The diversity of birds, as an urban ecosystem indicator, reflects ecosystem services and is impacted by urban development. To explore the impacts of urbanization on bird diversity, stratified to songbirds, terrestrial birds, climbers, swimming birds, wading birds, and raptors, we specifically investigated the existing and potential distributions of selected bird species, analyzed different contributions of environmental factors, and compared these with urban biodiversity conservation policies. We used bird records from the China Birdwatching Record Center (over 1400 species of birds for querying) and remotely-sensed landcover data, based on the MaxEnt model, to analyze bird spatial distribution characteristics and potential habitat corridors throughout Beijing. The results showed that: (1) Songbirds and terrestrial birds were predominantly concentrated in water areas in urban areas. Wading birds, climbers, swimming birds, and raptors were gathered in forest-covered areas, near wetlands and farmland in suburban areas. Projections indicated that the raptor species Common Kestrel (Falco tinnunculus) showed a notable shift toward urban cores. (2) Among climbers, Gray-headed Pygmy Woodpecker (Dendrocopos canicapillus) occupied the highest proportion of high-quality habitats (10.34%), contrasting with the representative songbird species Blackbird (Turdus merula) at 1.38%, which demonstrated adaptability to urban environments. Critical habitats were concentrated in shrub forests, supporting habitat connectivity. Proximity to water bodies was critical for raptors, wading, swimming, and climbers, whereas terrestrial birds and songbirds were more affected by artificial lighting. (3) The “urban and suburban park rings” policy has effectively enhanced habitat quality and connectivity, promoting urban biodiversity resilience. This study improves our understanding of how different bird communities adapt to urbanization in terms of habitats and movement corridors, and provides useful information for formulating urban bird biodiversity conservation strategies. Full article
Show Figures

Figure 1

Figure 1
<p>Location and land use of Beijing (Map of China from China Standard Map, <a href="http://bzdt.ch.mnr.gov.cn/" target="_blank">http://bzdt.ch.mnr.gov.cn/</a>, produced by the Ministry of Natural Resources of China, accessed on 1 November 2024).</p>
Full article ">Figure 2
<p>Bird ecological network assessment framework.</p>
Full article ">Figure 3
<p>Quantitative variation in the areas of bird habitats from 2015 to 2020 in Beijing.</p>
Full article ">Figure 4
<p>Spatiotemporal variation in the density distribution of birds from 2015 to 2020 in Beijing.</p>
Full article ">Figure 5
<p>Spatiotemporal variation in the density distribution of six bird communities in Beijing in 2019. ((<b>a</b>): songbirds; (<b>b</b>): terrestrial birds; (<b>c</b>): climbers; (<b>d</b>): swimming birds; (<b>e</b>): wading birds; (<b>f</b>): raptors).</p>
Full article ">Figure 6
<p>Predicted spatiotemporal variation in the density distribution of representative bird species in Beijing ((<b>a</b>): <span class="html-italic">Corvus macrorhynchos</span>; (<b>b</b>): <span class="html-italic">Turdus merula</span>; (<b>c</b>): <span class="html-italic">Streptopelia chinensis</span>; (<b>d</b>): <span class="html-italic">Dendrocopos canicapillus</span>; (<b>e</b>): <span class="html-italic">Alcedo atthis</span>; (<b>f</b>): <span class="html-italic">Aix galericulata</span>; (<b>g</b>): <span class="html-italic">Gallinula chloropus</span>; (<b>h</b>): <span class="html-italic">Ardea alba</span>; (<b>i</b>): <span class="html-italic">Ardea cinerea</span>; (<b>j</b>): <span class="html-italic">Falco tinnunculus</span>).</p>
Full article ">Figure 7
<p>Spatiotemporal distribution of critical habitats and potential habitat corridors for representative bird species in Beijing ((<b>a</b>): <span class="html-italic">Turdus merula</span>; (<b>b</b>): <span class="html-italic">Streptopelia chinensis</span>; (<b>c</b>): <span class="html-italic">Dendrocopos canicapillus</span>; (<b>d</b>): <span class="html-italic">Aix galericulata</span>; (<b>e</b>): <span class="html-italic">Ardea alba</span>; (<b>f</b>): <span class="html-italic">Falco tinnunculus</span>).</p>
Full article ">Figure 8
<p>The quantity of potential corridor areas for six representative bird species in Beijing.</p>
Full article ">Figure 9
<p>Proportion of various qualities of habitat available to the six bird species in Beijing.</p>
Full article ">Figure 10
<p>Proportion of elements in LULC on potential habitat corridors of six bird species in Beijing.</p>
Full article ">Figure 11
<p>Comparison of contribution rates of environmental factors of birds in the study area.</p>
Full article ">Figure 12
<p>Comparison results of potential habitat corridors for birds with the Beijing Urban Overall Plan (2016–2035).</p>
Full article ">
21 pages, 4171 KiB  
Article
Global and Regional Sex-Related Differences, Asymmetry, and Peak Age of Brain Myelination in Healthy Adults
by Marina Y. Khodanovich, Mikhail V. Svetlik, Anna V. Naumova, Anna V. Usova, Valentina Y. Pashkevich, Marina V. Moshkina, Maria M. Shadrina, Daria A. Kamaeva, Victoria B. Obukhovskaya, Nadezhda G. Kataeva, Anastasia Y. Levina, Yana A. Tumentceva and Vasily L. Yarnykh
J. Clin. Med. 2024, 13(23), 7065; https://doi.org/10.3390/jcm13237065 - 22 Nov 2024
Viewed by 745
Abstract
Background: The fundamental question of normal brain myelination in human is still poorly understood. Methods: Age-dependent global, regional, and interhemispheric sex-related differences in brain myelination of 42 (19 men, 23 women) healthy adults (19–67 years) were explored using the MRI method of [...] Read more.
Background: The fundamental question of normal brain myelination in human is still poorly understood. Methods: Age-dependent global, regional, and interhemispheric sex-related differences in brain myelination of 42 (19 men, 23 women) healthy adults (19–67 years) were explored using the MRI method of fast macromolecular fraction (MPF) mapping. Results: Higher brain myelination in males compared to females was found in global white matter (WM), most WM tracts, juxtacortical WM regions, and putamen. The largest differences between men and women, exceeding 4%, were observed bilaterally in the frontal juxtacortical WM; angular, inferior occipital, and cuneus WM; external capsule; and inferior and superior fronto-orbital fasciculi. The majority of hemispheric differences in MPF were common to men and women. Sex-specific interhemispheric differences were found in juxtacortical WM; men more often had left-sided asymmetry, while women had right-sided asymmetry. Most regions of deep gray matter (GM), juxtacortical WM, and WM tracts (except for projection pathways) showed a later peak age of myelination in women compared to men, with a difference of 3.5 years on average. Body mass index (BMI) was associated with higher MPF and later peak age of myelination independent of age and sex. Conclusions: MPF mapping showed high sensitivity to assess sex-related differences in normal brain myelination, providing the basis for using this method in clinics. Full article
(This article belongs to the Special Issue Neuroimaging in 2024 and Beyond)
Show Figures

Figure 1

Figure 1
<p>Example MPF map (<b>a</b>) and corresponding masks used for global measurements: cerebrospinal fluid (CSF) (<b>b</b>), GM (<b>c</b>), WM (<b>e</b>), and mixed WM–GM (<b>d</b>).</p>
Full article ">Figure 2
<p>An example of an individual MPF map segmentation obtained by registration of T1 Eve template [<a href="#B63-jcm-13-07065" class="html-bibr">63</a>] to MPF map. Slices are shown in axial, sagittal, and coronal projections. Different colors indicate regional segmentation of separate brain structures of juxtacortical WM, WM pathways, allocortex, deep GM, and brainstem.</p>
Full article ">Figure 3
<p>Sex-related global differences in brain myelination and volumes for WM, GM, mixed WM–GM, and CSF compartments. (<b>a</b>) Absolute volume differences in GM, WM, mixed WM–GM, and CSF. (<b>b</b>) Percentage differences in GM, WM, mixed WM–GM, and CSF. (<b>c</b>) Ratio of WM volume to GM volume. (<b>d</b>) Absolute MPF differences in global GM, WM, and mixed WM–GM. (<b>e</b>) Percentage MPF differences in global GM, WM, and mixed WM–GM. Error bars denote standard deviation. Significant differences: *—<span class="html-italic">p</span> &lt; 0.05, **—<span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 4
<p>Sex-related differences between MPF measurements in men and women for the separate brain regions: (<b>a</b>) juxtacortical WM of left and right hemispheres, (<b>b</b>) WM pathways in left and right hemispheres, (<b>c</b>) allocortex and deep GM of left and right hemispheres, (<b>d</b>) brainstem. Significant differences between men and women: *—<span class="html-italic">p</span> &lt; 0.05, **—<span class="html-italic">p</span> &lt; 0.01, ***—<span class="html-italic">p</span> &lt; 0.001. Significant differences between left and right hemispheres: +—<span class="html-italic">p</span> &lt; 0.05, ++—<span class="html-italic">p</span> &lt; 0.01, +++—<span class="html-italic">p</span> &lt; 0.001. The significance of the differences is marked on the side of the hemisphere in which the MPF is larger. Error bars correspond to SD.</p>
Full article ">Figure 5
<p>Sex-related percentage differences in average MPF measurements for women compared with men in the separate brain regions: (<b>a</b>) juxtacortical WM of left and right hemispheres, (<b>b</b>) WM pathways of left and right hemispheres, (<b>c</b>) allocortex and deep GM of left and right hemispheres, (<b>d</b>) brainstem. Significant differences between men and women: *—<span class="html-italic">p</span> &lt; 0.05, **—<span class="html-italic">p</span> &lt; 0.01, ***—<span class="html-italic">p</span> &lt; 0.001. Error bars correspond to SD.</p>
Full article ">Figure 6
<p>Regions of significant sex-related differences between MPF measurements in women and men in representative cross-sections of a 3D MPF map at different levels of axial (<b>a</b>) and coronal (<b>b</b>) projections of an individual MPF map. Regions of significantly higher MPF in men are marked by red (&gt;3%) and pink (&lt;3%) colors. L—left hemisphere, R—right hemisphere.</p>
Full article ">Figure 7
<p>Regions of significant interhemispheric differences in MPF measurements for men (<b>a</b>) and women (<b>b</b>) at different levels of axial projections of an individual MPF map. Symmetrical areas of the right (R) and left (L) hemispheres with significantly higher and lower MPF values are marked by a more saturated vs. paler tone of red for women, blue for men, and purple for similar interhemispheric differences for men and women.</p>
Full article ">Figure 8
<p>Examples of the best-fitting quadratic curves of age regressed on MPF in the right caudate nucleus (<b>a</b>) and the right body of the CC (<b>b</b>) for total sample (left), men (center), and women (right). Each scatterplot includes the quadratic regression equation. All shown regression equations are significant (<span class="html-italic">p</span> &lt; 0.05). The peak age calculated from the regression equations: (<b>a</b>) the caudate nucleus—38 years for the total sample, 36.4 years for men, and 43.8 years for women; (<b>b</b>) the body of the CC—39.3 years for the total sample, 37.6 years for men, and 43.5 years for women.</p>
Full article ">
25 pages, 2762 KiB  
Article
Construction and Demolition Waste Generation Prediction by Using Artificial Neural Networks and Metaheuristic Algorithms
by Ruba Awad, Cenk Budayan and Asli Pelin Gurgun
Buildings 2024, 14(11), 3695; https://doi.org/10.3390/buildings14113695 - 20 Nov 2024
Viewed by 1158
Abstract
In the actual estimation of construction and demolition waste (C&DW), it is significantly relevant to effective management, design, and planning at project stages, but the lack of reliable estimation methods and historical data prevents the estimation of C&DW quantities for both short- and [...] Read more.
In the actual estimation of construction and demolition waste (C&DW), it is significantly relevant to effective management, design, and planning at project stages, but the lack of reliable estimation methods and historical data prevents the estimation of C&DW quantities for both short- and long-term planning. To address this gap, this study aims to predict C&DW quantities in construction projects more accurately by integrating the gray wolf optimization algorithm (GWO) and the Archimedes optimization algorithm (AOA) into an artificial neural network (ANN). This study uses data concerning the actual quantities of work in 200 real-life construction and demolition projects performed in the Gaza Strip. Different performance parameters, such as mean absolute error (MAE), mean square error (MSE), root mean squared error (RMSE), and the coefficient of determination (R2), are used to evaluate the effectiveness of the models developed. The results of this study have shown that the AOA-ANN model outperforms the other models in terms of accuracy (R2 = 0.023728, MSE = 0.00056304, RMSE = 0.023728, MAE = 0.0086648). Moreover, this new hybrid model yields more accurate estimations of C&DW quantities with minimal input parameters, making the process of estimation more feasible. Full article
Show Figures

Figure 1

Figure 1
<p>Research methodology.</p>
Full article ">Figure 2
<p>Profile of the participated respondents.</p>
Full article ">Figure 3
<p>Flowchart of the proposed GWO-ANN algorithm.</p>
Full article ">Figure 4
<p>Flowchart of the proposed AOA-ANN algorithm.</p>
Full article ">Figure 5
<p>Frequency analysis of categorical variables.</p>
Full article ">Figure 6
<p>The scatter plot graphical visualization for developed ANN models with different nodes.</p>
Full article ">Figure 7
<p>The scatter plot graphical visualization for the different developed models for testing datasets.</p>
Full article ">Figure 7 Cont.
<p>The scatter plot graphical visualization for the different developed models for testing datasets.</p>
Full article ">Figure 7 Cont.
<p>The scatter plot graphical visualization for the different developed models for testing datasets.</p>
Full article ">Figure 8
<p>Comprehensive performance analysis of the best models: (<b>a</b>) the Taylor diagram and (<b>b</b>) scatter chart for the best developed models for testing datasets.</p>
Full article ">
16 pages, 570 KiB  
Article
Multi-Objective Optimization of Steel Pipe Pile Cofferdam Construction Based on Improved Sparrow Search Algorithm
by Zaolong Jiang, Chengfang Yang and Hongbo Yue
Appl. Sci. 2024, 14(22), 10407; https://doi.org/10.3390/app142210407 - 12 Nov 2024
Viewed by 615
Abstract
This paper develops a multi-objective optimization model to address the absence of systematic and practical evaluation methods for selecting construction schemes for steel pipe pile cofferdams. The model aims to minimize duration and cost while maximizing quality. Additionally, it proposes an improved sparrow [...] Read more.
This paper develops a multi-objective optimization model to address the absence of systematic and practical evaluation methods for selecting construction schemes for steel pipe pile cofferdams. The model aims to minimize duration and cost while maximizing quality. Additionally, it proposes an improved sparrow search algorithm (ISSA) to solve this problem. First, a tent chaotic map is introduced to initialize the sparrow population, enhancing the diversity of the initial population. Second, the principle of non-dominated ordering is introduced to sort the parent and offspring populations during the iteration process, and the appropriate individuals are selected to form the offspring population. Finally, gray correlation analysis is applied to optimize the Pareto solution set and determine the final construction scheme. The effectiveness and superiority of the ISSA is verified by using the Changsha Jinan Avenue project as a case study. The results indicate that the quality of the optimized construction scheme remains at a high level of 0.90 or more; the duration is shortened by 18 days, a reduction of 21%; and the total cost is reduced by CNY 220,000, saving 3% of the cost. Full article
Show Figures

Figure 1

Figure 1
<p>Map of construction’s hydrogeological conditions.</p>
Full article ">Figure 2
<p>Network diagram for construction scheme.</p>
Full article ">Figure 3
<p>Duration optimization iteration curves.</p>
Full article ">Figure 4
<p>Cost optimization iteration curves.</p>
Full article ">Figure 5
<p>Quality optimization iterative curves.</p>
Full article ">Figure 6
<p>Integrated duration–cost–quality optimization results.</p>
Full article ">
25 pages, 21810 KiB  
Article
Morphofunctional Features of Glomeruli and Nephrons After Exposure to Electrons at Different Doses: Oxidative Stress, Inflammation, Apoptosis
by Grigory Demyashkin, Sergey Koryakin, Mikhail Parshenkov, Polina Skovorodko, Matvey Vadyukhin, Zhanna Uruskhanova, Yulia Stepanova, Vladimir Shchekin, Artem Mirontsev, Vera Rostovskaya, Sergey Ivanov, Petr Shegay and Andrei Kaprin
Curr. Issues Mol. Biol. 2024, 46(11), 12608-12632; https://doi.org/10.3390/cimb46110748 - 6 Nov 2024
Viewed by 1049
Abstract
Kidney disease has emerged as a significant global health issue, projected to become the fifth-leading cause of years of life lost by 2040. The kidneys, being highly radiosensitive, are vulnerable to damage from various forms of radiation, including gamma (γ) and X-rays. However, [...] Read more.
Kidney disease has emerged as a significant global health issue, projected to become the fifth-leading cause of years of life lost by 2040. The kidneys, being highly radiosensitive, are vulnerable to damage from various forms of radiation, including gamma (γ) and X-rays. However, the effects of electron radiation on renal tissues remain poorly understood. Given the localized energy deposition of electron beams, this study seeks to investigate the dose-dependent morphological and molecular changes in the kidneys following electron irradiation, aiming to address the gap in knowledge regarding its impact on renal structures. The primary aim of this study is to conduct a detailed morphological and molecular analysis of the kidneys following localized electron irradiation at different doses, to better understand the dose-dependent effects on renal tissue structure and function in an experimental model. Male Wistar rats (n = 75) were divided into five groups, including a control group and four experimental groups receiving 2, 4, 6, or 8 Gray (Gy) of localized electron irradiation to the kidneys. Biochemical markers of inflammation (interleukin-1 beta [IL-1β], interleukin-6 [IL-6], interleukin-10 [IL-10], tumor necrosis factor-alpha [TNF-α]) and oxidative stress (malondialdehyde [MDA], superoxide dismutase [SOD], glutathione [GSH]) were measured, and morphological changes were assessed using histological and immunohistochemical techniques (TUNEL assay, caspase-3). The study revealed a significant dose-dependent increase in oxidative stress, inflammation, and renal tissue damage. Higher doses of irradiation resulted in increased apoptosis, early stages of fibrosis (at high doses), and morphological changes in renal tissue. This study highlights the dose-dependent effects of electrons on renal structures, emphasizing the need for careful consideration of the dosage in clinical use to minimize adverse effects on renal function. Full article
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
Show Figures

Figure 1

Figure 1
<p>Design of the experiment. Special notations: (<b>A</b>)—Male Wistar rats (9–10 weeks old) were randomly assigned to five groups after a 7-day quarantine period. These groups included one control group (intact) and four experimental groups, each receiving a different dose of electron irradiation (2 Gy, 4 Gy, 6 Gy, and 8 Gy) targeted at the abdomino-pelvic region; (<b>B</b>)—irradiation was performed using a NOVAC-11 pulsed electron accelerator. Specific doses were administered with careful shielding to protect surrounding tissues; (<b>C</b>)—following irradiation, blood samples were collected from the animals for biochemical analysis (7 days post-irradiation). The evaluation of blood biochemical parameters was conducted according to the established research methodology; (<b>D</b>)—morphological examinations and organ homogenate studies were performed post-irradiation, following the procedures detailed in the research methodology (7 days post-irradiation).</p>
Full article ">Figure 2
<p>Specialized patented restraint devices (sleds), developed by the Laboratory of Radiation Pathomorphology of the A.F. Tsyb Medical Radiological Research Center.</p>
Full article ">Figure 3
<p>Comparison of body weight and kidney mass across experimental groups measured at 7 days post-irradiation. All data are presented as mean ± SD. Statistically significant differences are indicated by symbols: *—comparison with control group (<span class="html-italic">p</span> &lt; 0.05). (<b>A</b>) Body weight of animals in experimental groups: the body weight of animals decreased progressively with increasing doses of electron irradiation, with the most significant reduction observed at 8 Gy. (<b>B</b>) Kidney mass in experimental groups: kidney mass showed a dose-dependent decrease, with the highest reduction occurring at 8 Gy compared to the control group.</p>
Full article ">Figure 4
<p>Levels of different cytokines in blood of experimental groups measured at 7 days post-irradiation: (<b>A</b>)—data for IL-1β; (<b>B</b>)—data for IL-6; (<b>C</b>)—data for TNF-α; (<b>D</b>)—data for IL-10. Data are presented as mean ± SD. Experimental groups are numbered according to the study design. Statistically significant differences are indicated by symbols: *—comparison with control group (<span class="html-italic">p</span> &lt; 0.05); **—comparison with control group (<span class="html-italic">p</span> &lt; 0.01); ***—comparison with control group (<span class="html-italic">p</span> &lt; 0.001); †—comparison between Group II (2 Gy) and Group IV (8 Gy) (<span class="html-italic">p</span> &lt; 0.01).</p>
Full article ">Figure 4 Cont.
<p>Levels of different cytokines in blood of experimental groups measured at 7 days post-irradiation: (<b>A</b>)—data for IL-1β; (<b>B</b>)—data for IL-6; (<b>C</b>)—data for TNF-α; (<b>D</b>)—data for IL-10. Data are presented as mean ± SD. Experimental groups are numbered according to the study design. Statistically significant differences are indicated by symbols: *—comparison with control group (<span class="html-italic">p</span> &lt; 0.05); **—comparison with control group (<span class="html-italic">p</span> &lt; 0.01); ***—comparison with control group (<span class="html-italic">p</span> &lt; 0.001); †—comparison between Group II (2 Gy) and Group IV (8 Gy) (<span class="html-italic">p</span> &lt; 0.01).</p>
Full article ">Figure 5
<p>Levels of different markers of oxidative stress in kidney homogenate of experimental groups (7 days post-irradiation): (<b>A</b>)—data for MDA; (<b>B</b>)—data for SOD; (<b>C</b>)—data for GSH. Data are presented as mean ± SD. Experimental groups are numbered according to the study design. Statistically significant differences are indicated by symbols: *—comparison with control group (<span class="html-italic">p</span> &lt; 0.05); **—comparison with control group (<span class="html-italic">p</span> &lt; 0.01); ***—comparison with control group (<span class="html-italic">p</span> &lt; 0.001); †—comparison between group II (2 Gy) and group IV (8 Gy) (<span class="html-italic">p</span> &lt; 0.01).</p>
Full article ">Figure 6
<p>A glomerulus of a rat from the control group; stain—hematoxylin and eosin, magnified ×400.</p>
Full article ">Figure 7
<p>Kidneys of rats from experimental groups at different radiation doses, evaluated 7 days post-irradiation; stain—hematoxylin and eosin; different magnification. On the slides: dilation of Bowman’s capsule (*), vacuolization (∆), dystrophic changes in nephron tubules (<b>□</b>), perivascular and periglomerular edema (◊), mild inflammatory (●).</p>
Full article ">Figure 7 Cont.
<p>Kidneys of rats from experimental groups at different radiation doses, evaluated 7 days post-irradiation; stain—hematoxylin and eosin; different magnification. On the slides: dilation of Bowman’s capsule (*), vacuolization (∆), dystrophic changes in nephron tubules (<b>□</b>), perivascular and periglomerular edema (◊), mild inflammatory (●).</p>
Full article ">Figure 8
<p>Kidneys of rats from experimental groups at different radiation doses, evaluated 7 days post-irradiation; stain—Masson’s trichrome; magn. ×40.</p>
Full article ">Figure 9
<p>The kidney of a rat from 8 Gy group (7 days post-irradiation); stain—Masson’s trichrome; magn.: left ×100, right ×200. On the slides: mild fibrosis (*).</p>
Full article ">Figure 10
<p>TUNEL staining of kidney tissue of all experiment groups (7 days post-irradiation): TUNEL-positive cells (green, pointers are green arrows); DAPI-positive cells (blue cells); scale bar = 50 μm, 70 μm and 80 μm.</p>
Full article ">Figure 10 Cont.
<p>TUNEL staining of kidney tissue of all experiment groups (7 days post-irradiation): TUNEL-positive cells (green, pointers are green arrows); DAPI-positive cells (blue cells); scale bar = 50 μm, 70 μm and 80 μm.</p>
Full article ">Figure 11
<p>Quantitative distribution of TUNEL-positive cells in kidney tissue sections after electron irradiation (7 days post-irradiation). Data are presented as mean ± SD. Experimental groups are numbered according to the study design. Statistically significant differences are indicated by symbols: *—comparison with control group (<span class="html-italic">p</span> &lt; 0.05); **—comparison with control group (<span class="html-italic">p</span> &lt; 0.01); ***—comparison with control group (<span class="html-italic">p</span> &lt; 0.001); ø—comparison between group II (2 Gy electron dose) and group IV (8 Gy electron dose) (<span class="html-italic">p</span> &lt; 0.001).</p>
Full article ">Figure 12
<p>Kidneys from the control and experimental groups: (<b>A</b>)—immunohistochemical reactions with antibodies to caspase-3, magnification ×40; scale bar—45 μm, 50 μm, 65 μm; (<b>B</b>)—quantification of caspase-3-positive cells in renal tissue according to the immunohistochemical analysis, graph: (<b>a</b>)—caspase-3-positive cells in the renal medulla; (<b>b</b>)—caspase-3-positive cells in the proximal and distal tubules of nephrons; (<b>c</b>)—in the tubules of the loop of Henle and the collecting ducts. Experimental groups are numbered according to the study design. All data are presented as mean ± SD. Statistically significant differences are indicated by symbols: *—comparison with control group (<span class="html-italic">p</span> &lt; 0.05); **—comparison with control group (<span class="html-italic">p</span> &lt; 0.01); ***—comparison with control group (<span class="html-italic">p</span> &lt; 0.001); †—comparison between group II (2 Gy) and group IV (8 Gy) (<span class="html-italic">p</span> &lt; 0.01).</p>
Full article ">Figure 13
<p>Illustration of the mechanism of radiation-induced nephropathy after electron irradiation (based on the specific literature): (<b>A</b>)—selection of animals for the study; (<b>B</b>)—irradiation of experimental animals using specialized facilities (different irradiation modes are possible); (<b>C</b>)—initiation of DNA double-strand breakdown; (<b>D</b>)—cascade of molecular and cellular reactions leading to direct disease formation (of varying severity).</p>
Full article ">
15 pages, 2464 KiB  
Article
Grade Classification of Camellia Seed Oil Based on Hyperspectral Imaging Technology
by Yuqi Gu, Jianhua Wu, Yijun Guo, Sheng Hu, Kaixuan Li, Yuqian Shang, Liwei Bao, Muhammad Hassan and Chao Zhao
Foods 2024, 13(20), 3331; https://doi.org/10.3390/foods13203331 - 20 Oct 2024
Cited by 2 | Viewed by 949
Abstract
To achieve the rapid grade classification of camellia seed oil, hyperspectral imaging technology was used to acquire hyperspectral images of three distinct grades of camellia seed oil. The spectral and image information collected by the hyperspectral imaging technology was preprocessed by different methods. [...] Read more.
To achieve the rapid grade classification of camellia seed oil, hyperspectral imaging technology was used to acquire hyperspectral images of three distinct grades of camellia seed oil. The spectral and image information collected by the hyperspectral imaging technology was preprocessed by different methods. The characteristic wavelength selection in this study included the continuous projections algorithm (SPA) and competitive adaptive reweighted sampling (CARS), and the gray-level co-occurrence matrix (GLCM) algorithm was used to extract the texture features of camellia seed oil at the characteristic wavelength. Combined with genetic algorithm (GA) and support vector machine algorithm (SVM), different grade classification models for camellia seed oil were developed using full wavelengths (GA-SVM), characteristic wavelengths (CARS-GA-SVM), and fusing spectral and image features (CARS-GLCM-GA-SVM). The results show that the CARS-GLCM-GA-SVM model, which combined spectral and image information, had the best classification effect, and the accuracy of the calibration set and prediction set of the CARS-GLCM-GA-SVM model were 98.30% and 96.61%, respectively. Compared with the CARS-GA-SVM model, the accuracy of the calibration set and prediction set were improved by 10.75% and 12.04%, respectively. Compared with the GA-SVM model, the accuracy of the calibration set and prediction set were improved by 18.28% and 18.15%, respectively. The research showed that hyperspectral imaging technology can rapidly classify camellia seed oil grades. Full article
Show Figures

Figure 1

Figure 1
<p>The diagram of the hyperspectral imaging system.</p>
Full article ">Figure 2
<p>The process of hyperspectral information extraction.</p>
Full article ">Figure 3
<p>Flow chart of SVM optimized by GA for the grade classification model of camellia seed oil.</p>
Full article ">Figure 4
<p>The grade classification results of PCA for camellia seed oil after SNV preprocessing.</p>
Full article ">Figure 5
<p>Grade classification prediction result of the CARS-GA-SVM model.</p>
Full article ">Figure 6
<p>Grade classification prediction result of the CARS-GLCM-GA-SVM model.</p>
Full article ">
28 pages, 8626 KiB  
Article
Research on the Coupling and Coordination of Land Ecological Security and High-Quality Agricultural Development in the Han River Basin
by Yuelong Su, Yucheng Liu, Yong Zhou and Jiakang Liu
Land 2024, 13(10), 1666; https://doi.org/10.3390/land13101666 - 13 Oct 2024
Cited by 1 | Viewed by 1205
Abstract
This study aims to investigate the coupling and harmonization between land ecological security (LES) and high-quality agricultural development (HAD) in the Han River Basin (HRB), China, with the objective of promoting harmonious coexistence between agriculture and ecosystems. Using 17 cities in the HRB [...] Read more.
This study aims to investigate the coupling and harmonization between land ecological security (LES) and high-quality agricultural development (HAD) in the Han River Basin (HRB), China, with the objective of promoting harmonious coexistence between agriculture and ecosystems. Using 17 cities in the HRB as the research objects, an evaluation index system of two systems, LES and HAD, was constructed, analyzed, and evaluated via projective tracer modeling for multiple intelligent genetic algorithms (MIGA-PTM). The degree of coupling coordination (DCC) was used to quantitatively evaluate the coupling coordination development status of the two systems, the obstacle model (OM) was used to identify the main influencing factors, and the gray predictive model first-order univariate model (GM (1, 1)) was used to predict the DCC of the LES and HAD from 2025 to 2040. The results show the following: (1) the LES and HAD levels of the 17 cities in the HRB tended to increase during the study period, and there was a large gap between cities; (2) the spatial distributions of the DCCs of the LES and HAD in the HRB were uneven, with high values in the southern and low values in the central and northern parts, and the overall degree of coupling tended to fluctuate. The overall DCC showed a fluctuating upward trend; (3) the degree of obstacles, per capita water resources, greening coverage, and rate of return on financial expenditure are the main influencing factors; and (4) the prediction results of GM (1, 1) indicate that the LES and HAD of the HRB will be close to reaching the intermediate stage of coupling in 2035. This research offers critical insights into sustainable development practices that facilitate the alignment of agricultural growth with ecological preservation. Full article
Show Figures

Figure 1

Figure 1
<p>Technology roadmap for LES and HAD evaluation and coupling studies.</p>
Full article ">Figure 2
<p>Study area.</p>
Full article ">Figure 3
<p>Trend map of LES changes in the HRB.</p>
Full article ">Figure 4
<p>Spatial differences in the LES results of the HRB.</p>
Full article ">Figure 5
<p>Elliptical distribution of the standard deviation of the LES and the change in the center of gravity in the HRB.</p>
Full article ">Figure 6
<p>Temporal distribution of the level of HAD in the HRB.</p>
Full article ">Figure 7
<p>Spatial differentiation of the HAD of the HRB.</p>
Full article ">Figure 8
<p>Elliptical distribution of the standard deviation of HAD and the change in the center of gravity in the HRB.</p>
Full article ">Figure 9
<p>Heatmap of the coupled coordination of LES and high-quality agricultural development in the HRB.</p>
Full article ">Figure 10
<p>Trends in the spatial and temporal evolution of the coupled and coordinated LES and HAD in the HRB.</p>
Full article ">
16 pages, 5022 KiB  
Article
The Role of the Mu Opioid Receptors of the Medial Prefrontal Cortex in the Modulation of Analgesia Induced by Acute Restraint Stress in Male Mice
by Yinan Du, Yukui Zhao, Aozhuo Zhang, Zhiwei Li, Chunling Wei, Qiaohua Zheng, Yanning Qiao, Yihui Liu, Wei Ren, Jing Han, Zongpeng Sun, Weiping Hu and Zhiqiang Liu
Int. J. Mol. Sci. 2024, 25(18), 9774; https://doi.org/10.3390/ijms25189774 - 10 Sep 2024
Viewed by 1072
Abstract
Mu opioid receptors (MORs) represent a vital mechanism related to the modulation of stress-induced analgesia (SIA). Previous studies have reported on the gamma-aminobutyric acid (GABA)ergic “disinhibition” mechanisms of MORs on the descending pain modulatory pathway of SIA induced in the midbrain. However, the [...] Read more.
Mu opioid receptors (MORs) represent a vital mechanism related to the modulation of stress-induced analgesia (SIA). Previous studies have reported on the gamma-aminobutyric acid (GABA)ergic “disinhibition” mechanisms of MORs on the descending pain modulatory pathway of SIA induced in the midbrain. However, the role of the MORs expressed in the medial prefrontal cortex (mPFC), one of the main cortical areas participating in pain modulation, in SIA remains completely unknown. In this study, we investigated the contributions of MORs expressed on glutamatergic (MORGlut) and GABAergic (MORGABA) neurons of the medial prefrontal cortex (mPFC), as well as the functional role and activity of neurons projecting from the mPFC to the periaqueductal gray (PAG) region, in male mice. We achieved this through a combination of hot-plate tests, c-fos staining, and 1 h acute restraint stress exposure tests. The results showed that our acute restraint stress protocol produced mPFC MOR-dependent SIA effects. In particular, MORGABA was found to play a major role in modulating the effects of SIA, whereas MORGlut seemed to be unconnected to the process. We also found that mPFC–PAG projections were efficiently activated and played key roles in the effects of SIA, and their activation was mediated by MORGABA to a large extent. These results indicated that the activation of mPFC MORGABA due to restraint stress was able to activate mPFC–PAG projections in a potential “disinhibition” pathway that produced analgesic effects. These findings provide a potential theoretical basis for pain treatment or drug screening targeting the mPFC. Full article
(This article belongs to the Special Issue The Multiple Mechanisms Underlying Neuropathic Pain (III))
Show Figures

Figure 1

Figure 1
<p>Acute restraint stress-induced analgesia (SIA). (<b>A</b>) Diagram of the stress and hot-plate test (HPT) procedures. (<b>B</b>) Effects of acute restraint stress on analgesia as assessed via an HPT; <span class="html-italic">n</span> = 9 for each group; ** <span class="html-italic">p</span> &lt; 0.01. (<b>C</b>) The percentage of the maximum possible effect (MPE%) from (<b>B</b>), calculated as MPE% = (post-test latency − pre-test latency)/(cut-off latency − pre-test latency) × 100%), the same below; the group data are shown as means ± SEMs; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 2
<p>The role of Mu opioid receptors (MORs) in the medial prefrontal cortex (mPFC) in SIA induced through acute restraint. (<b>A</b>) Flow diagram of the generation of MOR KO and MOR WT mice. (<b>B</b>) (<b>Left</b>): Schematic of in situ hybridization for <span class="html-italic">Oprm1</span> mRNA in the areas containing the mPFC in MOR KO and MOR WT mice. The <span class="html-italic">Oprm1</span> mRNA was stained in red, while the nucleus was stained in blue (DAPI). Scale bar = 500 µm. (<b>Right</b>): Higher-magnification images of the fields in the mPFC areas of MOR KO and MOR WT mice. Bar = 20 µm. (<b>C</b>) Quantitative analysis of the percentage of neurocyte MORs expressed in the mPFCs of MOR KO and MOR WT mice; <span class="html-italic">n</span> = 3 fields containing the median position of the right mPFC (acquired from different <span class="html-italic">n</span> = 3 animals) were used per group; MOR-positive cells and total neural cells were counted in each field; the form of data presentation was the MOR-positive cell/neural cell ratio. ** <span class="html-italic">p</span> &lt; 0.01. (<b>D</b>) Diagram of adeno-associated virus (rAAV) injection, stress, and HPT procedures. (<b>E</b>) Contributions of mPFC MORs to SIA induced through acute restraint as assessed via an HPT; <span class="html-italic">n</span> = 8 for each group; ** <span class="html-italic">p</span> &lt; 0.01. (<b>F</b>) Equated MPE% from the groups in (<b>E</b>), and data are shown as means ± SEMs; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 3
<p>MORGlut in the mPFC plays a marginal part in the modulation of SIA induced by acute restraint. (<b>A</b>) Diagram of the generation of MORGlut cKO and MORGlut WT mice. (<b>B</b>) (<b>Left</b>): Schematic of in situ hybridization for <span class="html-italic">Oprm1</span> mRNA in the areas containing mPFC in MORGlut cKO and MORGlut WT mice. The <span class="html-italic">Oprm1</span> mRNA was stained in red, <span class="html-italic">vGlut1</span> mRNA was stained in green, and the nuclei were stained in blue (DAPI). Bar = 500 µm. (<b>Right</b>): Higher-magnification images of the fields in the mPFC areas in MORGlut cKO and MORGlut WT mice. The white arrowhead indicates a double-labeled cell with <span class="html-italic">Oprm1</span> mRNA and <span class="html-italic">vGlut1</span> mRNA, the yellow arrowheads represent <span class="html-italic">Oprm1</span> mRNA localization in <span class="html-italic">vGlut1</span>-negative cells, and the purple arrowheads represent <span class="html-italic">vGlut1</span>-positive cells without Oprm1 mRNA. Bar = 20 µm. (<b>C</b>) Quantitative analysis of the percentage of MORGlut expressed in the mPFCs of MORGlut KO and MORGlut WT mice; <span class="html-italic">n</span> = 3 fields containing the median position of the right mPFC (acquired from different <span class="html-italic">n</span> = 3 animals) were used per group; the double-positive cells and total Glut-positive cells were counted in each field; the form of data presentation was the double-positive cell/Glut-positive cell ratio; ** <span class="html-italic">p</span> &lt; 0.01. (<b>D</b>) Flow diagram of the rAAV injection, stress, and HPT procedures. (<b>E</b>) Rare contributions of mPFC MORGlut to SIA induced by acute restraint as assessed via an HPT; <span class="html-italic">n</span> = 7 for each group; ** <span class="html-italic">p</span> &lt; 0.01. (<b>F</b>) Equated MPE% from the groups in (<b>E</b>); data are shown as means ± SEMs.</p>
Full article ">Figure 4
<p>Contributions of mPFC MORGABA to SIA induced by acute restraint. (<b>A</b>) Diagram detailing the generation of MORGABA cKO and MORGABA WT mice. (<b>B</b>) (<b>Left</b>): Schematic of in situ hybridization for <span class="html-italic">Oprm1</span> mRNA in the areas containing mPFC in MORGABA cKO and MORGABA WT mice. The <span class="html-italic">Oprm1</span> mRNA was stained in red, <span class="html-italic">vGAT</span> mRNA was stained in green, and nuclei were stained in blue (DAPI). Bar = 500 µm. (<b>Right</b>): Higher-magnification images of the fields in the mPFC areas in MORGABA cKO and MORGABA WT mice. The white arrowhead indicates a double-labeled cell with <span class="html-italic">Oprm1</span> mRNA and <span class="html-italic">vGAT</span> mRNA, the yellow arrowheads represent <span class="html-italic">Oprm1</span> mRNA localization in <span class="html-italic">vGAT</span>-negative cells, and the purple arrowheads represent <span class="html-italic">vGAT</span>-positive cells without Oprm1 mRNA. Bar = 20 µm. (<b>C</b>) Quantitative analysis of the percentage of MORGABA expressed in the mPFCs of MORGABA KO and MORGABA WT mice; <span class="html-italic">n</span> = 3 fields containing the median position of the right mPFC (acquired from different <span class="html-italic">n</span> = 3 animals) were used per group; the double-positive cells and total GAT-positive were counted in each field; the form of data presentation was the double-positive cell/GAT-positive cell ratio; ** <span class="html-italic">p</span> &lt; 0.01. (<b>D</b>) Flow diagram of the rAAV injection, stress, and HPT procedures. (<b>E</b>) Contributions of mPFC MORGABA to SIA induced by acute restraint as assessed via an HPT; <span class="html-italic">n</span> = 8 for each group; * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01. (<b>F</b>) Equated MPE% from the groups in (<b>E</b>), and the data are shown as means ± SEMs; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 5
<p>mPFC–periaqueductal gray (PAG) projections are significant in the process of inducing SIA through acute restraint. (<b>A</b>) Diagram showing the labeling of mPFC–PAG projections. (<b>B</b>) Typical morphology of labeled mPFC–PAG projections. Bar = 200 µm. (<b>C</b>) (<b>Left</b>): Representative images showing c-fos expression in mPFC–PAG projections in our stressed and unstressed groups of mice. Bar = 100 µm. (<b>Right</b>): Higher-magnification images of the fields from the left. The white arrowhead indicates a double-labeled cell with labeled mPFC–PAG projections and c-fos. Bar = 20 µm. (<b>D</b>) Quantitative analysis of the percentage of c-fos positive-labeled mPFC–PAG projections from the mPFCs of mice in the stressed and unstressed groups; <span class="html-italic">n</span> = 3 fields containing the median position of the right mPFC (acquired from different <span class="html-italic">n</span> = 3 animals) were used per group; the c-fos-positive EYFP cells and total EYFP cells were counted in each field; the form of data presentation was the c-fos-positive EYFP cell/EYFP cell ratio. ** <span class="html-italic">p</span> &lt; 0.01. (<b>E</b>) Diagram detailing the mounting of an inhibitory chemogenetical module in mPFC–PAG projections and a flow diagram of rAAV injection, cannula insertion, CNO injection, stress, and HPT procedures. (<b>F</b>) The influence of the chemogenetic inhibition of mPFC–PAG projections on SIA induced by acute restraint as assessed via an HPT; <span class="html-italic">n</span> = 6 for each group; ** <span class="html-italic">p</span> &lt; 0.01. (<b>G</b>) Equated MPE% from the groups in (<b>F</b>), and data are shown as means ± SEM; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 6
<p>MORGABA modulated the activity of mPFC–PAG projections during MOR-dependent SIA. (<b>A</b>) Diagram detailing the labeling of mPFC–PAG projections in MORGABA cKO and MORGABA WT mice. (<b>B</b>) Representative higher-magnification images showing c-fos expression in mPFC–PAG projections for MORGABA cKO (<b>B<sub>1</sub></b>) and MORGABA WT (<b>B<sub>2</sub></b>) mice under acute restraint stress or unstressed conditions. Bar = 20 µm; <span class="html-italic">n</span> = 3 fields containing the median position of the right mPFC (acquired from different <span class="html-italic">n</span> = 3 animals) were used per group; The white arrowheads represent co-labeling of EYFP and c-fos, the c-fos-positive EYFP cells and total EYFP cells were counted in each field; the form of data presentation was the c-fos-positive EYFP cell/EYFP cell ratio; ** <span class="html-italic">p</span> &lt; 0.01. (<b>C</b>) Quantitative analysis of the percentage of c-fos(+)-labeled mPFC–PAG projections in the mPFCs of MORGABA cKO (<b>C<sub>1</sub></b>) or MORGABA WT (<b>C<sub>2</sub></b>) mice under acute restraint stress or unstressed conditions; 3 fields were quantified from 3 animals; ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">
Back to TopTop