Hindawi Publishing Corporation Bioinorganic Chemistry and Applications Volume 2014, Article ID 718175, 11 pages http://dx.doi.org/10.1155/2014/718175 Research Article Synthesis, Characterization, and Biological Activity of N 󸀠-[(Z)-(3-Methyl-5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4ylidene)(phenyl)methyl]benzohydrazide and Its Co(II), Ni(II), and Cu(II) Complexes Jonnie N. Asegbeloyin,1 Oguejiofo T. Ujam,1 Emmanuel C. Okafor,1 Ilknur Babahan,2 Esin Poyrazoglu Coban,3 Ali Özmen,3 and Halil Biyik3 1 Department of Pure and Industrial Chemistry, University of Nigeria, Enugu State, Nsukka 410001, Enugu State, Nigeria Department of Chemistry, Adnan Menderes University, 09010 Aydin, Turkey 3 Department of Biology, Adnan Menderes University, 09010 Aydin, Turkey 2 Correspondence should be addressed to Jonnie N. Asegbeloyin; joniyi2001@yahoo.com Received 3 April 2014; Accepted 10 June 2014; Published 15 September 2014 Academic Editor: Nick Katsaros Copyright © 2014 Jonnie N. Asegbeloyin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Reaction of 1-phenyl-3-methyl-4-benzoyl-pyrazol-5-one and benzoyl hydrazide in refluxing ethanol gave N 󸀠 -[(Z)-(3-methyl5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)(phenyl)methyl]benzohydrazide (HL1 ), which was characterized by NMR spectroscopy and single-crystal X-ray structure study. X-ray diffraction analyses of the crystals revealed a nonplanar molecule, existing in the keto-amine form, with intermolecular hydrogen bonding forming a seven-membered ring system. The reaction of HL1 with Co(II), Ni(II), and Cu(II) halides gave the corresponding complexes, which were characterized by elemental analysis, molar conductance, magnetic measurements, and infrared and electronic spectral studies. The compounds were screened for their in vitro cytotoxic activity against HL-60 human promyelocytic leukemia cells and antimicrobial activity against some bacteria and yeasts. Results showed that the compounds are potent against HL-60 cells with the IC50 value ≤5 𝜇M, while some of the compounds were active against few studied Gram-positive bacteria. 1. Introduction Compounds derived from 4-acylpyrazol-5-one have continued to receive considerable attention due to their pharmaceutical importance and high biological activity [1–3]. They also enjoy a range of applications as a substructure in dye chemistry and synthesis [4]. The facile incorporation of conjugated systems with special features into the pyrazolone core leads to the formation of compounds with intense colour. Hydrazones and other Schiff bases are among the most studied 4-acylpyrazol-5-one derivatives, more so considering the fact that molecules with hydrazine moiety have been noted for their biological activity [5], especially as potential inhibitors for many enzymes [6]. The interest in the chemistry of 4-acylpyrazol-5-one Schiff bases stems from their interesting ligating ability due to the presence of multielectron rich donor atoms [7–9]. This is augmented by their tendency to exist in various tautomeric forms [10–12]. The ability of this class of compounds to form coordination compounds with a wide range of transition metals has been utilized in analytical chemistry, where they have been used as effective chelating and extracting reagents for many metal ions [13–15]. The literature is replete with studies of 4acylpyrazol-5-one Schiff bases and their metal complexes. However, the study of biological activities of this class of compounds and their metal complexes is largely restricted to studies on microorganisms. Few reports are available in the literature on the study of their anticancer properties [16, 17]. With the increasing search for nonplatinum based 2 anticancer drugs with less side effects, and similar, or better cytotoxicity, it is imperative to investigate the possibility of the use of 4-acylpyrazolone derivatives for cancer chemotherapy. In this paper, we report synthesis and characterization of N 󸀠 -[(Z)-(3-methyl-5-oxo-1-phenyl-1,5-dihydro4H-pyrazol-4-ylidene)(phenyl)methyl]benzohydrazide and its Co(II), Ni(II) and Cu(II) complexes, the in vitro antimicrobial activity against some bacteria and yeasts, and their antiproliferative effect on human promyelocytic leukemia cells. Bioinorganic Chemistry and Applications dimethyl formamide (DMF), followed by slow evaporation of the solution at room temperature for 1 week. 2. Experimental 2.3. Synthesis of Ni(II), Cu(II) and Co(II) Metal Complexes of HL1 . To an ethanolic solution (20 mL) of N 󸀠 -[(Z)-(3-methyl5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)(phenyl) methyl]benzohydrazide (0.43 g, 0.001 mol), ethanolic solution (10 mL) of the metal chloride (0.001 mol) was added with constant stirring. The coloured mixture was then refluxed for 4 h. The resulting metal complex was filtered hot, washed with boiling mixture of 1 : 1 water/ethanol, dried under suction, and kept in vacuum over CaCl2 . 2.1. Chemicals and Instrumentation. All the solvents are of analytical grade and were used without further purification. 1-phenyl-3-methylpyrazol-5-one, methyl benzoate and hydrazine mono-hydrate were used as supplied by Fluka. 1-phenyl-3-methyl-4-benzoyl-pyrazol-5-one and benzoylhydrazide were synthesized in accordance with reported procedures [18, 19]. Elemental analyses of C, H and N were performed using Carlo Erba Elemental analyzer EA 1108. Melting points were obtained with a Fisher John melting point apparatus. Magnetic moments were done on a magnetic susceptibility balance—Sherwood Scientific Cambridge, Model No.MK-I. The molar conductance measurements of the metal complexes were done in DMF/DMSO using Innolab conductivity meter Level 1. The percentage of metal in the complexes was determined using an Agilent ICP-MS7500Ce. IR spectra were recorded on a Perkin Elmer Spectrum 100 at the University of Waikato, Hamilton, New Zealand. 1 H and 13 C NMR spectra were obtained from a Bruker AV 500 MHz for 1 H and 125 MHz for 13 C using a 5 mm quadra nuclei probe (QNP). The X-ray crystallographic data were obtained at the University of Auckland on a Bruker SMART APEX II diffraction equipped with a CCD area detector, using MoK𝛼 radiation (𝜆 = 0.71073 Å). The software SMART was used for the collection of data frames, for indexing reflections, and to determine lattice parameters; SAINT was used for the integration of the intensity of the reflections and for scaling [20]. SADABS was used for empirical absorption correction [21]. The structures were solved using SHELXS97, and SHELXL-97 was also used for structure refinements and reporting [22]. The structures were refined by fullmatrix least-squares based on Fo 2 with anisotropic thermal parameters for non-hydrogen atoms. 2.4. Microorganisms and Condition for Cultivation. Fifteen bacteria and five yeast cultures were used in the study. Ten bacterial strains and two yeast strains were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). Other microorganism strains were obtained from Adnan Menderes University Faculty of Medicine. The Gram-negative (G−) were Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028, Proteus sp., Serratia marcescens, and Enterobacter sp. and the Gram-positive (G+) were Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 6633, Bacillus thuringiensis, Enterococcus faecalis ATCC 29212, Enterococcus faecalis ATCC 51299, Streptococcus pneumoniae ATCC 49617, and Listeria monocytogenes. Also, yeast strains such as Candida utilis, Candida albicans ATCC 90028, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae ATCC 9763 were used. Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Salmonella typhimurium ATCC 14028, Listeria monocytogenes, Proteus sp., Serratia marcescens, and Enterobacter sp. were cultured in nutrient broth (NB) (Merck) at 37∘ C; Streptococcus pneumoniae ATCC 49617, Enterococcus faecalis ATCC 29212, and Enterococcus faecalis ATCC 51299 were cultured in brain heart infusion broth (BHIB) (Merck) at 37∘ C for 24 h.; Micrococcus luteus ATCC 9341, Bacillus cereus ATCC 11778, Bacillus subtilis ATCC 6633, and Bacillus thuringiensis were cultured in nutrient broth (NB) (Merck) at 30∘ C; Candida utilis, Candida albicans ATCC 90028, Candida glabrata, Candida tropicalis, and Saccharomyces cerevisiae ATCC 9763 were cultured in malt extract broth (MEB) (Merck, USA) at 30∘ C for 24 h. 2.2. Synthesis of 𝑁󸀠 -[(Z)-(3-Methyl-5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)(phenyl)methyl] benzohydrazide (HL1 ). Synthesis and crystallization of HL1 was done by a modification of literature procedure [23] as follows. A solution of 4-benzoyl-3-methyl-1-phenylpyrazol-5-one (2.78 g; 0.01 mol) in 30 mL ethanol was mixed with a solution of benzoyl hydrazide (1.36 g; 0.01 mol) in ethanol (20 mL). The mixture refluxed for 2 h and cooled (Figure 1). The yellow precipitate formed was isolated by gravity filtration and recrystallized from ethanol. Crystals suitable for X-ray crystallographic analysis were obtained by slow dissolution of HL1 in ethanol by warming and addition of few drops of 2.5. Antimicrobial Assay. The antimicrobial activities of all the synthesized compounds were determined by the disc diffusion method [24, 25], and the minimum inhibitory concentration (MIC) was obtained by broth dilution method [25]. 2.6. Disc Diffusion Method. The standard method of Antimicrobial Disc Susceptibility Tests reported by the National Committee for Clinical Laboratory Standards [25] was used. Fresh stock solutions (1000 𝜇g⋅mL−1 ) of all the synthesized compounds were prepared in DMSO. The inoculum suspensions of the tested bacteria and yeasts were prepared from the Bioinorganic Chemistry and Applications 3 O H2 N O H3 C NH NH O H3 C CH3 CH2 OH + N O N N N Reflux O N 2 3 1 3 2 1 4 O O NH NH N H3 C N H3 C 15 H3 C 5 16 N N N OH H N N O 6 NH 6 17 19 NH 7 N 18 20 21 O 8 9 14 10 O 13 11 12 24 23 22 4 5 Figure 1: Synthesis of HL1 . broth cultures (18–24 h) and the turbidity equivalent adjusted to 0.5 McFarland standard tube to give a concentration of 1 × 108 bacterial cells and 1 × 106 yeast cells/mL. To test the antimicrobial activity of all the synthesized compounds, a Mueller Hinton Agar (MHA) plate was inoculated with 0.1 mL broth culture of bacteria or yeast. Then a hole of 6 mm in diameter and depth was made on top with a sterile stick and filled with 50 𝜇L of synthesized compounds. Plates inoculated with E. coli ATCC 25922, S. typhimurium ATCC 14028, S. aureus ATCC 25923, S. epidermidis ATCC 12228, E. faecalis ATCC 29212, Enterococcus faecalis ATCC 51299, S. pneumoniae ATCC 49617, Listeria monocytogenes, Proteus sp., S. marcescens, and Enterobacter sp. were incubated at 37∘ C for 24 h, while those with M. luteus ATCC 9341, Bacillus subtilis ATCC 6633, B. cereus ATCC 11778, B. thuringiensis, S. cerevisiae ATCC 9763, C. albicans ATCC 90028, C. glabrata, C. utilis, and C. tropicalis were incubated at 30∘ C for 24 h. At the end of incubation time, the diameters of the inhibition zones formed on the MHA were evaluated in millimeters. Disc of chloramphenicol (C30), gentamicin (CN10), tetracycline (TE30), erythromycin (E15), ampicillin (AMP10), and nystatin (NS100) were used as positive controls. The measured inhibition zones of the study compounds were compared with those of the reference discs. 2.7. Minimum Inhibitory Concentration (MIC). Minimum inhibitory concentration (MIC) was determined by reported method [25]. The study bacteria were inoculated in nutrient broth and incubated at 30–37∘ C for 24 hr while the yeasts were inoculated in Malt Extract Broth and incubated at 30∘ C for 48 h. The inoculums were adjusted according to 0.5 McFarland standard tube. Initially, 100 𝜇L of Mueller Hinton Broth (MHB) was placed in each well. After, the compounds were dissolved in DMSO (2 mg mL−1 ) and transferred into the first well. Two fold serial dilution of the compounds were carried out to determine the MIC, within the concentration range 256 to 0.125 𝜇g mL−1 . Cultures were grown at 30–37∘ C (18–20 h) and the final inoculum was approximately 106 cfu mL−1 . The lowest concentration of the study compounds that resulted in complete inhibition of the microorganisms was represented as MIC (𝜇g mL−1 ). Similar procedures were done on positive controls, streptomycin (I. E. Ulagay). In each case, the test was performed in triplicate and the results were expressed as means. 2.8. Cell Antiproliferation Test. HL-60 promyelocytic leukaemia cells were purchased from ATCC. Cells were grown in RPMI-1640 medium supplemented with 10% heat inactivated fetal calf serum, 1% L-glutamine, and 1% penicillin/CO2 . 4 Bioinorganic Chemistry and Applications Table 1: Selected bond lengths (Å) and bond angles (∘ ) for HL1 (estimated standard deviations are in brackets). O(1)-C(8) O(2)-C(18) N(1)-C(7) N(1)-N(2) N(2)-C(8) C(7)-N(1)-N(2) C(8)-N(2)-N(1) C(16)-N(3)-N(4) C(18)-N(4)-N(3) C(18)-N(4)-C(19) N(3)-N(4)-C(19) Bond lengths and atomic distances (Å) 1.2396(9) N(3)-C(16) 1.2655(9) N(3)-N(4) 1.3299(10) N(4)-C(18) 1.3820(9) N(4)-C(19) 1.3487(10) C(1)-C(2) Bond angles (∘ ) 123.33(7) N(1)-C(7)-C(17) 119.20(7) N(1)-C(7)-C(6) 106.36(6) O(1)-C(8)-N(2) 112.14(6) O(1)-C(8)-C(14) 129.16(7) N(2)-C(8)-C(14) 118.67(6) O(2)-C(18)-N(4) All media and supplements were obtained from Life Technologies. HL 60 cells were seeded in T-25 tissue culture flasks at a concentration of 1 × 105 /mL and incubated with increasing concentrations (5, 10, 20, and 40 𝜇M) of the study compounds. Cell counts and IC50 values were determined at 24 and 72 h using a Thoma slide. Experiments were done in triplicate. The percentage of cell divisions compared to the untreated control was calculated as follows: [ (C72 h + drug − C24 h + drug) ] × 100 (C72 h − drug − C24 h − drug) (1) = % cell division, where C72 h + study compound is the cell number after 72 h of treatment with study compound, C24 h + compound is the cell number after 23 h of treatment with study compound, C72 h − compound is the cell number after 72 h without treatment with compound, and C24 h − compound is the cell number after 24 h without treatment with compound. 3. Results and Discussion 3.1. X-Ray Crystal Structure of HL1 . The crystal structure of HL1 has been reported in literature [23] but was redetermined to investigate if the new method of crystal isolation could affect the R-factor which was earlier reported as 0.0542 [23], and control the intramolecular rearrangement of the molecule from structure (4) to any of the other tautomers, see Figure 1. The data and structure refinement parameter details are given in Table 2. The crystal structure of HL1 was solved by direct method. The nitrogen and oxygen atoms were located followed by other atoms in subsequent refinements. All nonhydrogen atoms were refined as anisotropic. The Xray crystal structures and atom numbering schemes of HL1 are as shown in Figure 2. The selected bond lengths and angles are shown in Table 1. The crystal data and structure refinement details are presented in Table 2. The compound is a nonplanar molecule. The measured angle between the pyrazolone ring plane C(18) C(17) C(16) N(3) and C(6) C(1) C(5) plane is 71.4∘ . In addition the angle between C(8) N(2) 1.3097(11) 1.4003(10) 1.3766(10) 1.4140(10) 1.3899(13) 125.71(7) 112.99(7) 122.97(7) 122.98(7) 114.05(7) 124.30(7) C(3) C(4) C(5) C(2) C(1) C(6) C(15) C(7) C(16) C(17) N(3) C(18) N(2) N(4) C(8) C(24) C(19) O(2) C(14) N(1) O(1) C(9) C(10) C(11) C(13) C(12) C(20) C(21) C(23) C(22) Figure 2: Molecular structure of HL1 showing the atom numbering scheme with thermal ellipsoids at the 50% probability level. N(1) and C(6) C(7) N(1) planes is 33.6∘ . The phenacyl group adopts a gauche conformation about the N–N vector. The angle between the phenacyl C(13) C(14) C(9) and N(2) C(14) C(8) planes is 29.9∘ . The hydrazino nitrogen atoms adopted the trans-conformation which is more preferred when not in a ring system [26, 27]. The bond length of hydrazino (>N– N<) nitrogen atoms 1.3820(9) Å the carbonyl (>C=O) group 1.2396(9) Å is in agreement with literature reports [23, 28]. The ring carbon–oxygen O(2)–C(18)bond length 1.2655(9) Å is longer but in good agreement with earlier reports on the pyrazolone ring carbonyl group [23, 29]. The molecular structure of the compound indicated intermolecular hydrogen bonding between the hydrogen atom on N(2) and the O(2) forming a non planar seven membered ring system. Intramolecular hydrogen bonding is also observed in the molecule as shown in the crystal packing motif in Figure 3. 3.2. Analytical and Physical Data. All the metal complexes are colored and soluble in polar solvents (dimethyl sulfoxide, dimethyl formamide, and anhydrous ethanol) but insoluble in solvents such as hexane, pentane, tetrahydrofuran and Bioinorganic Chemistry and Applications 5 Table 2: Crystal data and structure refinement details for HL1 . Identification code Formula Formula weight Temperature (K) Wavelength (Å) Crystal system Space group a/Å b/Å c/Å 𝛼/∘ 𝛽/∘ 𝛾/∘ Volume (Å3 ) 𝑍 Calculated density (Mg/m3 ) Absorption coefficient (mm−1 ) 𝐹(000) Crystal size (mm3 ) Theta range for data collection Limiting indices Reflections collected/unique Completeness to theta Absorption correction Max. and min. transmission Refinement method Data/restraints/parameters Goodness of fit on 𝐹2 Final 𝑅 indices [𝐼 > 2𝜎(𝐼)] 𝑅 indices (all data) Largest diff. peak and hole HL 1 C24 H20 N4 O2 396.44 109(2) 0.71073 Triclinic P-1 9.0895(2) 10.9699(3) 11.0637(3) 89.5410(10) 78.6940(10) 66.0660(10) 985.59(4) 2 1.336 0.088 416 0.70 × 0.49 × 0.28 mm 2.50 to 34.28∘ −14 ≤ ℎ ≤ 13, −17 ≤ 𝑘 ≤ 16, −16 ≤ 𝑙 ≤ 17 29726/7289 [𝑅(int) = 0.0274] 27.50∘ 100.0% Multiscan 0.9759 and 0.9412 Full-matrix least-squares on 𝐹2 7289/0/278 1.028 𝑅1 = 0.0439, 𝑤𝑅2 = 0.1199 𝑅1 = 0.0535, 𝑤𝑅2 = 0.1282 0.440 and −0.289 e A−3 carbon tetrachloride. Analytical and physical data of all the study compounds are shown in Table 3. The complexes are nonelectrolytes in DMSO, as evident from the molar conductivity values which range from ∼9 to 13 Ohm−1 cm2 mol−1 [30–32]. 3.3. FT-Infrared Spectra. The relevant stretching frequencies are tabulated in Table 4. The absorption bands in the region ∼3000–3280 cm−1 in the spectral of HL1 and the metal complexes were assigned to NH stretching vibrations. Two strong v(C=O) bands are observable in the keto-amine form of HL1 , one assignable to v(C=O) of the lateral chain comprising the hydrazide moiety and the other to the v(C=O) of pyrazolone a o b O(1) O(2) c Figure 3: A view of the crystal packing of HL1 showing the intramolecular and intermolecular hydrogen bonding. ring [33–35]. These strong vibrational bands (cm−1 ) were observed at 1645 and 1596, respectively. The v(C=O) band assigned to the hydrazide moiety shifted to lower frequencies in the metal complexes, suggesting that the carbonyl oxygen was involved in coordination to the metal ions [36–38]. Bands in the region of 1566–1570 cm−1 have been assigned to imino v(C=N) in all the metal complexes. The bands in the region of 1437–1440 cm−1 in the metal complexes have been assigned to v(C–O) [33, 39] resulting from enolization and deprotonation before coordination [14, 40]. The bands in the region ∼1025–1120 cm−1 in the ligand and the metal complexes have been assigned to v(N–N). Bands in the range ∼510–520 cm−1 in the metal complexes were assigned to v(M–O) while bands in the range ∼420– 480 cm−1 were assigned to v(M–N) [41–43]. vO–H of water of crystallization was observed in the region ∼3370–3470 cm−1 in the metal complexes. HL1 reacted as the enol-imino form (Figure 1 (5)), when coordinated to metal ions in solution, coordinating via pyrazolone ring enolic (C–O), azomethine (C=N), and hydrazide side chain (C=O). 3.4. 1 H and 13 C NMR Spectra. The possible keto forms of HL1 are as shown in Figure 1. The X-ray structure confirmed that it crystallized in the keto-amine form (4). The conjugated system that extended from the pyrazolone ring confers extra stability on form (4). In the 1 H NMR spectrum the 3-methyl protons signal was observed as singlet upfield at 2.17 ppm. The phenyl protons appeared as multiplets in the range 7.26– 8.07 ppm while the signal due to –NH was observed at 8.83 ppm. The 13 C-NMR spectrum of the ligand exhibited 17 carbon signals comprising 16 sp2 and one sp3 (–CH3 ) carbons; the signal at 𝛿16.06 has been assigned to carbon of the pyrazolone core side 3-methyl. Carbons of benzene rings are present at 𝛿 118.47–165.18. The most deshielded carbons have been assigned relevant signals downfield. The assignments are as follows: C-1 = 127.55, C-2 = 127.42, C-3 = 129.70, C-4 = 127.42, C-5 = 127.55, C-6 = 131.43, C-7 = 138.75, C-8 = 163.64, C-9 = 130.69, C-10 = 148.27, C-11 = 132.78, C12 = 130.04, C-13 = 128.83, C-14 = 130.04, C-15 = 15.44, C-16 = 149.28, C-17 = 120.34, C-18 = 154.55, C-19 = 138.22, C-20 = 119.85, C-21 = 129.70, C-22 = 125.26, C-23 = 129.70 and C24 = 119.85 ppm (see (4) in Figure 1). All assignments were consistent with literature on related studies [44–48]. 6 Bioinorganic Chemistry and Applications Table 3: Elemental analysis and physical data of the ligand and complexes. Compound Colour HL1 MF Yellow Elemental analysis % found (calculated) C H N 72.68 5.21 14.28 (72.73) (5.05) (14.14) 66.34 4.98 12.99 (66.43) (4.65) (12.92) 66.78 4.81 13.02 (66.45) (4.65) (12.92) 65.61 5.15 13.41 (66.08) (4.62) (12.85) Yield % C24 H20 N4 O2 85 CoL1 2 ⋅H2 O Yellow C48 H40 N8 O5 Co 70 NiL1 2 ⋅H2 O Green C48 H40 N8 O5 Ni 67 CuL1 2 ⋅H2 O Green C46 H40 N8 O5 Cu 60 𝜇eff B.M M 𝜆𝑀 ohm−1 cm2 mol−1 Melting Point ∘ C — — — 196 6.35 (6.79) 6.60 (6.76) 7.63 (7.33) 4.93 11.56 286 2.94 12.50 272 1.90 10.34 260 Table 4: Relevant IR absorption bands for the ligand and metal complexes. Compound L1 Cu(L1 )2 ⋅H2 O Ni(L1 )2 ⋅H2 O Co(L1 )2 ⋅H2 O a VO–H — 3448 3376 3468 VN–H 3132 3311 3059 3275 VC=Oa 1645 1597 1621 1623 VC=Ob 1596 — — — VC=N — 1567 1570 1566 VN–N 1117 1026 1027 1025 VC–O — 1440 1437 1438 VM–O — 519 509 510 VM–N — 418 460 472 Hydrazide moiety, b pyrazolone ring. Table 5: Electronic spectra data of ligand and metal complexes. Compounds HL 1 Bands (cm−1 ) (𝜀, Lmol−1 cm−1 ) Assigned transition 42,533 (10600) 32,786 (7500) 29,411 (9100) 𝑛 󳨀→ 𝜋∗ 𝑛 󳨀→ 𝜋∗ 𝜋 󳨀→ 𝜋∗ CoL1 2 ⋅H2 O 21,276 (228) 20,408 (224) 10,204 (165) CuL1 2 ⋅H2 O 13,424(255) 16,354(135) 22,722 (245) NiL1 2 ⋅H2 O 23,809 (225) 14,174 (172) 12,545 (142) T1g (F) 󳨀→ 4 T1g (P) T1g (F) 󳨀→ 4 A2g (F) 4 T1g (F) 󳨀→ 4 T2g (F) 4 4 B1g 󳨀→ 2 A1g , B1g 󳨀→ 2 B2g 2 B1g 󳨀→ 2 Eg 2 2 A2g 󳨀→ 3 T1g (P) A2g 󳨀→ 3 T1g (F) 3 A2g 󳨀→ 3 T2g (F) 3 3 3.5. Electronic Spectra and Magnetic Studies. The significant electronic absorption bands in the spectra of the ligand and the metal complexes recorded in DMSO solution are presented in Table 5. The ligand shows high frequency bands at 42,533, 32,786, and 29,411 cm−1 which were assigned to n → 𝜋∗ and 𝜋 → 𝜋∗ transitions [49, 50]. For a typical d9 octahedral Cu2+ complex the 2 D free-ion term is split into two levels by the Oh symmetry and further split on distortion to D4h or C4v symmetry [51]. Three spin allowed transitions can be observed in the visible and near IR regions. In the present study the Cu(II) complex show bands at 16,354, 13,424 and 22,722 cm−1 which have been assigned to 2 B1g → 2 B2g , 2 B1g → 2 A1g and 2 B1g → 2 Eg transitions respectively [52– 54]. The observed magnetic moment of 1.90 B.M is suggestive of an octahedral geometry for Cu(II) complex [54]. Ni(II) with d8 configuration in an octahedral field has a ground state of 3 F, which is split into 3 A2g , 3 T2g , and 3 T1g (F) states and the excited state designated as 3 T1g (P). Three bands can be observed in the spectra. The Ni(II) complex displays three bands at 12,545, 14,174, and 23,809, cm−1 assignable to 3 A2g → 3 T2g (F), 3 A2g → 3 T1g (F), and 3 A2g → 3 T1g (P) transitions, respectively [55]. The magnetic moment of 2.93 B.M for Ni(II) complex is an indication of its octahedral geometry. The three bands observed at 21,276, 20,408, and 10,204 cm−1 for Co(II) complexes have been assigned to 4 T1g (F) → 4 T1g (P), 4 T1g (F) → 4 A2g (F) and 4 T1g (F) → 4 T2g (F) transitions, respectively [52]. The magnetic moment of 4.94 B.M and the observed electronic transitions indicate high spin octahedral geometry of the complex [56]. On the basis of microanalytical data, magnetic moments, conductivity measurements, and spectral analysis, Figure 4 has been proposed for the metal complexes. 3.6. Antimicrobial Test Results. The results of antimicrobial activities of the study compounds reported as inhibition zone diameter (mm) are showed in Table 6. The inhibition zone diameter of the reference antibiotics used on the test microorganisims are presented in Table A which can be found in Supplementary Material available online at http:// dx.doi.org/10.1155/2014/718175. The MIC values which suggested that some of the studied compounds exhibited appreciable antimicrobial activity are showed in Table 7. The ligand, Co(II) and Cu(II) complexes showed appreciable activities against some pathogen bacteria, while the Ni(II) complex did not show any antimicrobial activity. The ligand (HL1 ) showed significant antimicrobial activity against four Gram- positive bacteria Micrococcus luteus ATCC 9341, Bioinorganic Chemistry and Applications 7 NH H3 C N N O O N M N O O N N CH3 NH Figure 4: Proposed structures of the metal complexes {M = Co(II), Cu(II), Ni(II)}. 40 𝜇M (b) 150 100 100 CuL1 2 ·H2 O, DMSO (c) −50 5 𝜇M 0 Co + DMSO 40 𝜇M 20 𝜇M 10 𝜇M 5 𝜇M 0 50 Co 50 Proliferation (%) 150 Co + DMSO 40 𝜇M (a) Co 20 𝜇M NiL1 2 ·H2 O, DMSO HL1 , DMSO Proliferation (%) 20 𝜇M −50 10 𝜇M 0 10 𝜇M 40 𝜇M 20 𝜇M 10 𝜇M 5 𝜇M −50 Co + DMSO 0 50 5 𝜇M 50 100 Co + DMSO 100 Co Proliferation (%) 150 Co Proliferation (%) 150 CoL1 2 ·H2 O, DMSO (d) Figure 5: Inhibitory percentage (%) of compounds at 5–40 𝜇M on HL-60 cancer cell line. Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, and Bacillus subtilis ATCC 6633; the Co complex showed antimicrobial activity against two Gram-positive bacteria Bacillus cereus ATCC 11778 and Bacillus subtilis ATCC 6633. Cu(II) complex exhibited antimicrobial activity against three Gram-positive bacteria Micrococcus luteus ATCC 9341, Staphylococcus aureus ATCC 25923, and Bacillus cereus ATCC 11778. However, none of the study compounds exhibited antimicrobial activity against tested Gram-negative bacteria and yeasts. Gram-negative bacteria are noted for the protective lipopolysaccharide (LPS) in the outer membrane of their cell walls, which protects the sensitive inner membrane and the cell wall from drugs and dyes [57]. This protective lipopolysaccharide is absent in Gram-positive bacteria. In order for these compounds to exert bacteriostatic or bactericidal actions, they must access intracellular targets [58, 59]. 8 Bioinorganic Chemistry and Applications Table 6: Antimicrobial activities of HL1 and the metal complexes (inhibition zone diameter (mm). Test microorganism Escherichia coli ATCC 25922 Salmonella typhimurium ATCC 14028 Micrococcus luteus, ATCC 9341 Staphylococcus aureus ATCC 25923 Staphylococcus epidermidis ATCC 12228 Bacillus cereus ATCC 11778 Bacillus subtilis ATCC 6633 Bacillus thuringiensis∗ Enterococcus faecalis ATCC 29212 Enterococcus faecalis ATCC 51299 Streptococcus pneumoniae ATCC 49617 Proteus sp.∗ Serratia marcescens∗ Enterobacter sp.∗ Listeria monocytogenes∗∗ Candida albicans ATCC 90028 Candida utilis∗ Candida tropicalis∗ Candida glabrata∗ Saccharomyces cerevisiae ATCC 9763 HL1 CoL1 2 ⋅H2 O NiL1 2 ⋅H2 O CuL1 2 ⋅H2 O — — — — — — — — 15 — — 12 15 — — 14 — — — — 15 13 — 10 13 8 — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — — (—): zone of inhibition not included in the diameter of the well. ∗ Special gift from Faculty of Medicine, Adnan Menderes University. ∗∗ Food isolated. Table 7: Minimum inhibitory concentration of compounds (MIC, 𝜇g⋅mL−1 ). Test microorganisms Micrococcus luteus, ATCC 9341 Staphylococcus aureus ATCC 25923 Bacillus cereus ATCC 11778 Bacillus subtilis ATCC 6633 HL1 CoL1 2 ⋅H2 O CuL1 2 ⋅H2 O Str 4 NT 16 32 4 NT 4 32 4 4 64 64 8 128 NT 64 Note: Str: streptomycin. (NT): Not tested. Therefore in Gram-negative bacteria, these compounds must cross the outer membrane, a substantial permeability barrier and thus a major determinant of antimicrobial resistance in these bacteria [59]. The observed results of the antimicrobial tests suggest that the compounds were not able to permeate the protective outer membrane of the Gram negative bacteria to the extent of having antimicrobial effects. The observed better antimicrobial action exhibited by the ligand may be ascribed to the formation of hydrogen bonds with the active centre of cell constituents, resulting in interference with the normal function of the cell [60]. On coordination, the site for hydrogen bonding is minimized in the resultant metal complexes. MICs were measured for the compounds that showed appreciable growth inhibition zones; the study was restricted to microorganisms that were affected by the compounds. From the results of MIC tests shown in Table 7, it can be seen that MIC of selected compounds against bacterial pathogens varied from 4 to 128 𝜇g mL−1 . The lowest MICs were observed Bioinorganic Chemistry and Applications 9 Table 8: Antiproliferative activities of investigated compounds against human cancer (HL-60) cell line. Compounds IC50 (𝜇M) HL1 3.36 ± 1.12 CoL1 2 ⋅H2 O 2.52 ± 0.22 for HL1 (4–8 𝜇g mL−1 ) against the four tested microorganisms. Low MICs were also recorded for Cu complex (Staphylococcus aureus ATCC 25923) and Co complex (Bacillus cereus ATCC 11778). The results suggest that the compounds showed good antimicrobial activity against these organisms. 3.7. Results of Cell Proliferation Test. HL-60 (human promyelocytic leukemia cells) cell line is a leukemic cell line that has been used for laboratory research. The HL-60 cultured cell line provides a continuous source of human cells for studying the molecular events of myeloid differentiation and the effects of physiologic, pharmacologic, and virologic processes. The results of antiproliferative activities of investigated compounds against human cancer (HL-60) cell line are presented in Table 8. The results indicated that HL1 and its Cu(II) complex exhibit cytotoxic effects at the lowest tested concentration with IC50 value between ∼3 and 5 𝜇M while the Co(II) and Ni(II) complexes exhibit better cytotoxic effects with IC50 value ∼2-3 𝜇M. Figure 5 shows effects of different concentrations of tested compounds on HL-60 cell line. It is evident from Figure 5 that all the compounds (at <5–40 𝜇M) were able to inhibit the proliferation of more than 50% of the HL-60 cell. The tested compounds can be described as potent anticancer agents due to their considerably high antiproliferative effects. This is consistent with documented facts by the US NCI screening program, that if the IC50 value (following the incubation of a compound and cell lines between 48 and 72 h) is less than 10 𝜇M, the compound is considered to have in vitro cytotoxic activity [61]. 4. Conclusions A 4-acylpyrazolone hydrazone and some of its metal complexes have been synthesized and characterized. The crystal and spectra data showed that the ligand crystallized in the keto-amine form and coordinated as a monoanionic tridentate ONO donor ligand. Physicochemical and spectral data show that the metal to ligand ratio is 1 : 2 in the Ni(II), Cu(II), and Co(II) complexes. The electronic data and magnetic moments are in favour of octahedral geometry for Ni(II), Cu(II), and Co(II) complexes. The compounds exhibited better antimicrobial activity against Gram-positive bacterial strains studied. 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