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WO2009038079A1 - 細胞測定容器、細胞外電位測定方法、薬品検査方法 - Google Patents

細胞測定容器、細胞外電位測定方法、薬品検査方法 Download PDF

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Publication number
WO2009038079A1
WO2009038079A1 PCT/JP2008/066751 JP2008066751W WO2009038079A1 WO 2009038079 A1 WO2009038079 A1 WO 2009038079A1 JP 2008066751 W JP2008066751 W JP 2008066751W WO 2009038079 A1 WO2009038079 A1 WO 2009038079A1
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WIPO (PCT)
Prior art keywords
end portion
diameter part
well
detector
insertion hole
Prior art date
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PCT/JP2008/066751
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English (en)
French (fr)
Inventor
Yasuyuki Asai
Makoto Nagakura
Takeaki Fukami
Keisuke Oosumi
Original Assignee
Biotec Co., Ltd.
Reprocell, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Biotec Co., Ltd., Reprocell, Inc. filed Critical Biotec Co., Ltd.
Priority to EP08832478.5A priority Critical patent/EP2213721B1/en
Priority to US12/733,729 priority patent/US8702922B2/en
Priority to JP2009533151A priority patent/JP5528111B2/ja
Publication of WO2009038079A1 publication Critical patent/WO2009038079A1/ja

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/4833—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures
    • G01N33/4836—Physical analysis of biological material of solid biological material, e.g. tissue samples, cell cultures using multielectrode arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Optics & Photonics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

　従来のウエルプレートを用いた細胞観察では、検査にかかるコストが高かった。ウエルプレート１の天板部２ａに開口した各ウエル３は、内周面３２ａを円錐状に窪ませた縮径部３２を備えている。縮径部３２の下端部には、挿通孔３３が形成されている。検知部４は、丸棒状のリファレンス電極４１の外周面を、絶縁部４２で覆って構成されている。検知部４は、リファレンス電極４１の外周面を絶縁部４２で覆わずに外部に露出させた上端部４ａ及び下端部４ｂを備えている。下端部４ｂよりも上端側には、絶縁部４２の外周面を測定電極４３で覆った拡径部４ｃが設けられている。検知部４は、拡径部４ｃを挿通孔３３に挿通されてウエル３に固定され、上端部４ａをウエル３に収容されている。また、検知部４は、測定電極４３の上端面４３ａを、挿通孔３３の周縁に位置する縮経部３２の内周面３２ａと連ならせている。
PCT/JP2008/066751 2007-09-18 2008-09-17 細胞測定容器、細胞外電位測定方法、薬品検査方法 WO2009038079A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP08832478.5A EP2213721B1 (en) 2007-09-18 2008-09-17 Cell measuring vessel, extracellular potential measuring method, and chemical testing method
US12/733,729 US8702922B2 (en) 2007-09-18 2008-09-17 Cell measuring vessel, extracellular potential measuring method, and chemical testing method
JP2009533151A JP5528111B2 (ja) 2007-09-18 2008-09-17 細胞測定容器、細胞外電位測定方法、薬品検査方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007240868 2007-09-18
JP2007-240868 2007-09-18

Publications (1)

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WO2009038079A1 true WO2009038079A1 (ja) 2009-03-26

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PCT/JP2008/066751 WO2009038079A1 (ja) 2007-09-18 2008-09-17 細胞測定容器、細胞外電位測定方法、薬品検査方法

Country Status (4)

Country Link
US (1) US8702922B2 (ja)
EP (1) EP2213721B1 (ja)
JP (1) JP5528111B2 (ja)
WO (1) WO2009038079A1 (ja)

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JP2011015643A (ja) * 2009-07-09 2011-01-27 Reprocell Inc 細胞の膜電位測定用の培養液、及びそれを用いた培養方法、膜電位測定方法
WO2011010721A1 (ja) * 2009-07-24 2011-01-27 ニプロ株式会社 細胞電位測定容器及びその製造方法
WO2011010720A1 (ja) * 2009-07-24 2011-01-27 ニプロ株式会社 細胞電位測定容器
WO2011068057A1 (ja) * 2009-12-01 2011-06-09 ニプロ株式会社 細胞電位測定容器及びその製造方法
WO2012043820A1 (ja) * 2010-09-30 2012-04-05 国立大学法人東京医科歯科大学 心筋毒性検査および心筋細胞評価のための方法および装置
JP2012200225A (ja) * 2011-03-26 2012-10-22 Nipro Corp 細胞電位測定容器遠心分離補助具及び遠心分離方法
JP2013543726A (ja) * 2010-10-29 2013-12-09 エフ．ホフマン−ラ ロシュ アーゲー 不整脈のリスクを判定する方法
EP2427543A4 (en) * 2009-05-05 2017-11-29 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
CN109337814A (zh) * 2018-11-07 2019-02-15 深圳大学 细胞孔板
JP2020153733A (ja) * 2019-03-19 2020-09-24 国立研究開発法人物質・材料研究機構 自動電気化学測定システム、および、電気化学特性を自動測定する方法
WO2020235253A1 (ja) * 2019-05-20 2020-11-26 パナソニックＩｐマネジメント株式会社 選別方法、選別装置
WO2021095393A1 (ja) 2019-11-14 2021-05-20 Nok株式会社 細胞外電位測定装置
JP2021083369A (ja) * 2019-11-28 2021-06-03 学校法人立命館 測定方法及び測定装置
WO2023204018A1 (ja) * 2022-04-22 2023-10-26 株式会社Ｓｃｒｅｅｎホールディングス インピーダンス計測装置およびインピーダンス計測方法

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US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
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EP2291645B1 (en) 2008-05-05 2015-09-09 Acea Biosciences, Inc. Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
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JP6370544B2 (ja) * 2013-11-11 2018-08-08 浜松ホトニクス株式会社 細胞観察装置及び細胞観察方法
US20150172426A1 (en) * 2013-12-16 2015-06-18 Motorola Mobility Llc Antenna tuning correction for multiple rear housing materials
US12066428B2 (en) 2015-11-20 2024-08-20 Agilent Technologies, Inc. Cell-substrate impedance monitoring of cancer cells
JP6793918B2 (ja) * 2016-09-02 2020-12-02 日本光電工業株式会社 測定装置
US11746328B2 (en) 2017-03-03 2023-09-05 Agilent Technologies, Inc. Methods and systems for functional maturation of iPSC and ESC derived cardiomyocytes
US11458470B2 (en) * 2017-06-23 2022-10-04 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for analyzing platelet function
EP3502695A1 (en) * 2017-12-22 2019-06-26 IMEC vzw A method and a system for analysis of cardiomyocyte function
JP7469092B2 (ja) * 2020-03-23 2024-04-16 株式会社Ｓｃｒｅｅｎホールディングス 細胞保持容器
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EP2427543A4 (en) * 2009-05-05 2017-11-29 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
JP2011015643A (ja) * 2009-07-09 2011-01-27 Reprocell Inc 細胞の膜電位測定用の培養液、及びそれを用いた培養方法、膜電位測定方法
JP5823291B2 (ja) * 2009-07-24 2015-11-25 ニプロ株式会社 細胞電位測定容器
WO2011010720A1 (ja) * 2009-07-24 2011-01-27 ニプロ株式会社 細胞電位測定容器
JPWO2011010721A1 (ja) * 2009-07-24 2013-01-07 ニプロ株式会社 細胞電位測定容器及びその製造方法
US9381515B2 (en) 2009-07-24 2016-07-05 Nipro Corporation Container for measuring cell potential
WO2011010721A1 (ja) * 2009-07-24 2011-01-27 ニプロ株式会社 細胞電位測定容器及びその製造方法
WO2011068057A1 (ja) * 2009-12-01 2011-06-09 ニプロ株式会社 細胞電位測定容器及びその製造方法
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JPWO2012043820A1 (ja) * 2010-09-30 2014-02-24 国立大学法人 東京医科歯科大学 心筋毒性検査および心筋細胞評価のための方法および装置
WO2012043820A1 (ja) * 2010-09-30 2012-04-05 国立大学法人東京医科歯科大学 心筋毒性検査および心筋細胞評価のための方法および装置
US9447447B2 (en) 2010-09-30 2016-09-20 National University Corporation Tokyo Medical And Dental University Method and apparatus for testing cardiotoxicity and evaluating cardiomyocytes
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JP2012200225A (ja) * 2011-03-26 2012-10-22 Nipro Corp 細胞電位測定容器遠心分離補助具及び遠心分離方法
CN109337814A (zh) * 2018-11-07 2019-02-15 深圳大学 细胞孔板
CN109337814B (zh) * 2018-11-07 2024-05-14 深圳大学 细胞孔板
JP2020153733A (ja) * 2019-03-19 2020-09-24 国立研究開発法人物質・材料研究機構 自動電気化学測定システム、および、電気化学特性を自動測定する方法
JP7148079B2 (ja) 2019-03-19 2022-10-05 国立研究開発法人物質・材料研究機構 自動電気化学測定システム、および、電気化学特性を自動測定する方法
WO2020235253A1 (ja) * 2019-05-20 2020-11-26 パナソニックＩｐマネジメント株式会社 選別方法、選別装置
JP7570012B2 (ja) 2019-05-20 2024-10-21 パナソニックＩｐマネジメント株式会社 選別方法、選別装置
WO2021095393A1 (ja) 2019-11-14 2021-05-20 Nok株式会社 細胞外電位測定装置
US11946847B2 (en) 2019-11-14 2024-04-02 Nok Corporation Extracellular potential measurement device
JP2021083369A (ja) * 2019-11-28 2021-06-03 学校法人立命館 測定方法及び測定装置
JP7460110B2 (ja) 2019-11-28 2024-04-02 学校法人立命館 測定方法及び測定装置
WO2023204018A1 (ja) * 2022-04-22 2023-10-26 株式会社Ｓｃｒｅｅｎホールディングス インピーダンス計測装置およびインピーダンス計測方法

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JP5528111B2 (ja) 2014-06-25
EP2213721A1 (en) 2010-08-04
EP2213721A4 (en) 2013-04-10
US20100304423A1 (en) 2010-12-02
JPWO2009038079A1 (ja) 2011-01-06
EP2213721B1 (en) 2016-03-02
US8702922B2 (en) 2014-04-22

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