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WO2009066673A1 - 核酸分析用組成物 - Google Patents

核酸分析用組成物 Download PDF

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Publication number
WO2009066673A1
WO2009066673A1 PCT/JP2008/070975 JP2008070975W WO2009066673A1 WO 2009066673 A1 WO2009066673 A1 WO 2009066673A1 JP 2008070975 W JP2008070975 W JP 2008070975W WO 2009066673 A1 WO2009066673 A1 WO 2009066673A1
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
analysis
composition
reactivity
datp
Prior art date
Application number
PCT/JP2008/070975
Other languages
English (en)
French (fr)
Inventor
Shigeya Suzuki
Yukako Kodama
Keiko Kurosawa
Eiry Kobatake
Masayasu Mie
Original Assignee
Kikkoman Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kikkoman Corporation filed Critical Kikkoman Corporation
Priority to US12/734,378 priority Critical patent/US7989190B2/en
Priority to EP08851813A priority patent/EP2221386B1/en
Priority to CN200880116377.6A priority patent/CN101861397B/zh
Priority to JP2009542561A priority patent/JPWO2009066673A1/ja
Publication of WO2009066673A1 publication Critical patent/WO2009066673A1/ja
Priority to US13/134,899 priority patent/US20120295252A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

　本発明は、ｄＡＴＰαＳのような、ＤＮＡポリメラーゼに対して反応性が低く高価な試薬を用いることなく、ｄＡＴＰを用いた安価で高精度かつ高感度な核酸分析のためのホタルルシフェラーゼ、それを用いる核酸分析方法及び核酸分析キットを提供する。 　ｄＡＴＰに対する反応性がＡＴＰに対する反応性の１／４００以下であるルシフェラーゼを含む、核酸分析用組成物、上記の組成物を用いることを特徴とする、核酸分析方法、及び上記の組成物を含む、核酸分析用キット。
PCT/JP2008/070975 2007-11-20 2008-11-19 核酸分析用組成物 WO2009066673A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US12/734,378 US7989190B2 (en) 2007-11-20 2008-11-19 Composition for analyzing nucleic acid
EP08851813A EP2221386B1 (en) 2007-11-20 2008-11-19 Composition for analysis of nucleic acid
CN200880116377.6A CN101861397B (zh) 2007-11-20 2008-11-19 核酸分析用组合物
JP2009542561A JPWO2009066673A1 (ja) 2007-11-20 2008-11-19 核酸分析用組成物
US13/134,899 US20120295252A1 (en) 2007-11-20 2011-06-20 Composition for Analyzing Nucleic Acid

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-300113 2007-11-20
JP2007300113 2007-11-20
JP2008043830 2008-02-26
JP2008-043830 2008-02-26

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US13/134,899 Division US20120295252A1 (en) 2007-11-20 2011-06-20 Composition for Analyzing Nucleic Acid

Publications (1)

Publication Number Publication Date
WO2009066673A1 true WO2009066673A1 (ja) 2009-05-28

Family

ID=40667495

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/070975 WO2009066673A1 (ja) 2007-11-20 2008-11-19 核酸分析用組成物

Country Status (5)

Country Link
US (2) US7989190B2 (ja)
EP (2) EP2407551A3 (ja)
JP (1) JPWO2009066673A1 (ja)
CN (1) CN101861397B (ja)
WO (1) WO2009066673A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009140662A1 (en) * 2008-05-15 2009-11-19 Illumina, Inc. Engineered luciferases
CN103320404A (zh) * 2009-11-10 2013-09-25 龟甲万株式会社 萤火虫荧光素酶、其基因和萤火虫荧光素酶的制造法
WO2020009215A1 (ja) * 2018-07-06 2020-01-09 キッコーマン株式会社 ルシフェラーゼ変異体

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7175881B2 (ja) * 2016-10-13 2022-11-21 プロメガ コーポレイション ルシフェリン分解産物による阻害に対して改善された耐性を持つ熱安定性ルシフェラーゼ

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998013523A1 (en) 1996-09-27 1998-04-02 Pyrosequencing Ab Method of sequencing dna
JPH10512750A (ja) * 1995-01-20 1998-12-08 イギリス国 突然変異体ルシフェラーゼ
WO1999002697A1 (fr) * 1997-07-08 1999-01-21 Kikkoman Corporation Proteine mutante bioluminescente et son procede de production
US5891659A (en) 1996-03-04 1999-04-06 Kikkoman Corporation Bioluminescent adenosine phosphate ester assay and reagent
WO2002064830A2 (en) * 2001-02-14 2002-08-22 Cambridge University Technical Services Limited Method for detecting dna polymerisation
JP2003509601A (ja) 1999-09-16 2003-03-11 ティッセンクルップ・トランスラピート・ゲゼルシャフト・ミト・ベシュレンクテル・ハフツング トラックで拘束された車両のための走行路を製造するための、特に磁気浮上軌道を製造するための担持体及びこれにより製造される走行路
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
JP3749628B2 (ja) 1997-12-26 2006-03-01 キッコーマン株式会社 ルシフェラーゼおよびそれを用いる細胞内ａｔｐの測定法
JP2007068450A (ja) 2005-09-06 2007-03-22 Hitachi Ltd 段階反応を用いたｄｎａ塩基配列決定方法
JP2007097577A (ja) 2005-09-06 2007-04-19 Bioenex Inc 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6074859A (en) 1997-07-08 2000-06-13 Kikkoman Corporation Mutant-type bioluminescent protein, and process for producing the mutant-type bioluminescent protein
JP4782528B2 (ja) * 2005-10-04 2011-09-28 株式会社日立製作所 塩基配列決定方法と試薬
JP5188771B2 (ja) * 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
JP2009190687A (ja) * 2008-02-18 2009-08-27 Calsonic Kansei Corp 車両用空気調和システム
WO2009140662A1 (en) * 2008-05-15 2009-11-19 Illumina, Inc. Engineered luciferases
JP2010081908A (ja) * 2008-10-02 2010-04-15 Hitachi Ltd 変異型のホタルルシフェラーゼ

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3466765B2 (ja) 1994-07-27 2003-11-17 キッコーマン株式会社 ビオチン化ホタルルシフェラーゼ、ビオチン化ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、ビオチン化ホタルルシフェラーゼの製造法及び生物発光分析法
JPH10512750A (ja) * 1995-01-20 1998-12-08 イギリス国 突然変異体ルシフェラーゼ
US5891659A (en) 1996-03-04 1999-04-06 Kikkoman Corporation Bioluminescent adenosine phosphate ester assay and reagent
WO1998013523A1 (en) 1996-09-27 1998-04-02 Pyrosequencing Ab Method of sequencing dna
WO1999002697A1 (fr) * 1997-07-08 1999-01-21 Kikkoman Corporation Proteine mutante bioluminescente et son procede de production
JP3749628B2 (ja) 1997-12-26 2006-03-01 キッコーマン株式会社 ルシフェラーゼおよびそれを用いる細胞内ａｔｐの測定法
JP2003509601A (ja) 1999-09-16 2003-03-11 ティッセンクルップ・トランスラピート・ゲゼルシャフト・ミト・ベシュレンクテル・ハフツング トラックで拘束された車両のための走行路を製造するための、特に磁気浮上軌道を製造するための担持体及びこれにより製造される走行路
WO2002064830A2 (en) * 2001-02-14 2002-08-22 Cambridge University Technical Services Limited Method for detecting dna polymerisation
JP2007068450A (ja) 2005-09-06 2007-03-22 Hitachi Ltd 段階反応を用いたｄｎａ塩基配列決定方法
JP2007097577A (ja) 2005-09-06 2007-04-19 Bioenex Inc 変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
ANAL. BIOCHEM., vol. 151, no. 2, 1985, pages 504 - 509
ANAL. BIOCHEM., vol. 167, 1987, pages 235 - 238
ANAL. BIOCHEM., vol. 333, 2004, pages 296 - 302
BIOCHEMISTRY, vol. 42, 2003, pages 10429
BRANCHINI B.R. ET AL.: "A mutagenesis study of the putative luciferin binding site residues of firefly luciferase", BIOCHEMISTRY, vol. 42, no. 35, 2003, pages 10429 - 10436 *
MOYER J.D. ET AL.: "Nucleoside triphosphate specificity of firefly luciferase", ANAL. BIOCHEM., vol. 131, 1983, pages 187 - 189 *
NUCLEIC ACIDS RES., vol. 29, no. 19, 2001, pages 93
See also references of EP2221386A4 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009140662A1 (en) * 2008-05-15 2009-11-19 Illumina, Inc. Engineered luciferases
CN103320404A (zh) * 2009-11-10 2013-09-25 龟甲万株式会社 萤火虫荧光素酶、其基因和萤火虫荧光素酶的制造法
WO2020009215A1 (ja) * 2018-07-06 2020-01-09 キッコーマン株式会社 ルシフェラーゼ変異体

Also Published As

Publication number Publication date
CN101861397A (zh) 2010-10-13
CN101861397B (zh) 2014-04-16
EP2407551A3 (en) 2012-04-18
JPWO2009066673A1 (ja) 2011-04-07
EP2221386B1 (en) 2012-06-20
US20100255480A1 (en) 2010-10-07
EP2221386A4 (en) 2011-01-26
EP2407551A2 (en) 2012-01-18
EP2221386A1 (en) 2010-08-25
US7989190B2 (en) 2011-08-02
US20120295252A1 (en) 2012-11-22

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