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WO2002048674A3 - Methods, devices, arrays and kits for detecting and analyzing biomolecules - Google Patents

Methods, devices, arrays and kits for detecting and analyzing biomolecules Download PDF

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Publication number
WO2002048674A3
WO2002048674A3 PCT/US2001/044009 US0144009W WO0248674A3 WO 2002048674 A3 WO2002048674 A3 WO 2002048674A3 US 0144009 W US0144009 W US 0144009W WO 0248674 A3 WO0248674 A3 WO 0248674A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
methods
tissue
kits
arrays
Prior art date
Application number
PCT/US2001/044009
Other languages
French (fr)
Other versions
WO2002048674A2 (en
Inventor
Vladimir Knezevic
Michael R Emmert-Buck
Galina Baibakova
Dan-Paul Hartmann
Stephen Hewitt
Capre Denise Mitchell
Kevin Gardner
Original Assignee
20 20 Gene Systems Inc
Us Health
Vladimir Knezevic
Michael R Emmert-Buck
Galina Baibakova
Dan-Paul Hartmann
Stephen Hewitt
Capre Denise Mitchell
Kevin Gardner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/718,990 external-priority patent/US6602661B1/en
Priority claimed from US09/753,574 external-priority patent/US20020012920A1/en
Application filed by 20 20 Gene Systems Inc, Us Health, Vladimir Knezevic, Michael R Emmert-Buck, Galina Baibakova, Dan-Paul Hartmann, Stephen Hewitt, Capre Denise Mitchell, Kevin Gardner filed Critical 20 20 Gene Systems Inc
Priority to JP2002549932A priority Critical patent/JP2004518949A/en
Priority to AU2002243236A priority patent/AU2002243236B2/en
Priority to EP01989119A priority patent/EP1358314A4/en
Priority to US10/432,423 priority patent/US6969615B2/en
Priority to AU4323602A priority patent/AU4323602A/en
Priority to CA002428441A priority patent/CA2428441A1/en
Publication of WO2002048674A2 publication Critical patent/WO2002048674A2/en
Publication of WO2002048674A3 publication Critical patent/WO2002048674A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44739—Collecting the separated zones, e.g. blotting to a membrane or punching of gel spots
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Electrochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure is directed to devices, arrays, kits and methods for detecting biomolecules in a tissue section or other substantially two-dimensional sample (e.g., electrophoretic gel or cDNA microarray) by creating 'carbon copies' of the biomolecules eluted from the sample and visualizing the biomolecules on the copies using one or more detector molecules (e.g., antibodies or DNA probes) having specific affinity for the biomolecules of interest. Specific methods are provided for identifying the pattern of biomolecules in the samples. Other specific methods are provided for the identification and analysis of biological molecules produced by cells and/or tissue, especially human cells and/or tissue. The disclosure also provides a plurality of differentially prepared and/or processed membranes for use with the described methods, and which permit identification and analysis of biomolecules. For example, Figure 1 shows a menbrane array (13) in which molecules are transferred from a tissue section (11) using a wick (20).
PCT/US2001/044009 1999-07-26 2001-11-20 Methods, devices, arrays and kits for detecting and analyzing biomolecules WO2002048674A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2002549932A JP2004518949A (en) 2000-11-20 2001-11-20 Methods, devices, arrays, and kits for detecting and analyzing biomolecules
AU2002243236A AU2002243236B2 (en) 2000-11-20 2001-11-20 Methods, devices, arrays and kits for detecting and analyzing biomolecules
EP01989119A EP1358314A4 (en) 2000-11-20 2001-11-20 Methods, devices, arrays and kits for detecting and analyzing biomolecules
US10/432,423 US6969615B2 (en) 1999-07-26 2001-11-20 Methods, devices, arrays and kits for detecting and analyzing biomolecules
AU4323602A AU4323602A (en) 2000-11-20 2001-11-20 Methods, devices, arrays and kits for detecting and analyzing biomolecules
CA002428441A CA2428441A1 (en) 2000-11-20 2001-11-20 Methods, devices, arrays and kits for detecting and analyzing biomolecules

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
US09/718,990 US6602661B1 (en) 1999-07-26 2000-11-20 Methods and arrays for detecting biomolecules
US09/718,990 2000-11-20
US09/753,574 2001-01-04
US09/753,574 US20020012920A1 (en) 2000-11-20 2001-01-04 Method and kit for proteomic identification
US28625801P 2001-04-25 2001-04-25
US60/286,258 2001-04-25
US29647501P 2001-06-08 2001-06-08
US60/296,475 2001-06-08
US30403101P 2001-07-09 2001-07-09
US60/304,031 2001-07-09

Publications (2)

Publication Number Publication Date
WO2002048674A2 WO2002048674A2 (en) 2002-06-20
WO2002048674A3 true WO2002048674A3 (en) 2002-09-06

Family

ID=27540697

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/044009 WO2002048674A2 (en) 1999-07-26 2001-11-20 Methods, devices, arrays and kits for detecting and analyzing biomolecules

Country Status (5)

Country Link
EP (1) EP1358314A4 (en)
JP (1) JP2004518949A (en)
AU (2) AU2002243236B2 (en)
CA (1) CA2428441A1 (en)
WO (1) WO2002048674A2 (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838222B2 (en) * 1999-07-26 2010-11-23 United States of America/ NIH Methods, devices and kits for multiplex blotting of biological samples from multi-well plates
GB2391867A (en) * 2002-08-13 2004-02-18 Smartbead Technologies Ltd Analysis system using coded supports
JP4007269B2 (en) * 2003-07-29 2007-11-14 オムロン株式会社 Biomolecule shape transfer method, chip substrate manufacturing method, and biochip manufacturing method
US8012693B2 (en) 2003-12-16 2011-09-06 3M Innovative Properties Company Analysis of chemically crosslinked cellular samples
WO2005098432A1 (en) * 2004-04-05 2005-10-20 Proteome Systems Intellectual Property Pty Ltd Improved diagnostic testing apparatus
AU2005230642B2 (en) * 2004-04-05 2008-06-05 Proteome Systems Ltd. Improved diagnostic testing apparatus
GB2435510A (en) 2006-02-23 2007-08-29 Mologic Ltd Enzyme detection product and methods
GB2435511A (en) 2006-02-23 2007-08-29 Mologic Ltd Protease detection
GB2435512A (en) 2006-02-23 2007-08-29 Mologic Ltd A binding assay and assay device
US8075755B2 (en) * 2007-12-13 2011-12-13 Bio-Rad Laboratories, Inc. Polymeric sorbent sheets as ion reservoirs for electroblotting
JP2010096529A (en) * 2008-10-14 2010-04-30 Toppan Printing Co Ltd Transfer laminate, electrophoresis and transfer laminate and electrophoresis and transfer chip
US8524507B2 (en) 2009-08-19 2013-09-03 The Cleveland Clinic Foundation Method for detecting a target molecule in a biological sample
EP2400304A1 (en) * 2010-06-22 2011-12-28 Centro de Investigación Cooperativa En Biomateriales ( CIC biomaGUNE) Method for the characterization of intermolecular interactions
JP2013178140A (en) * 2012-02-28 2013-09-09 Sharp Corp Isolation device of nucleic acid-protein complex
WO2015078781A1 (en) * 2013-11-29 2015-06-04 Ge Healthcare Bio-Sciences Ab Integrated system for electro-blotting
IN2013DE03479A (en) 2013-11-29 2015-06-05 Ge Healthcare Bio Sciences Ab
GB201411624D0 (en) * 2014-06-30 2014-08-13 Ge Healthcare Uk Ltd Spatial molecular profiling of solid biological masses and profile storage
WO2019024990A1 (en) * 2017-08-01 2019-02-07 Albert-Ludwigs-Universität Freiburg Electrophoretic separation of biomolecules
CA3099267A1 (en) * 2018-05-08 2019-11-14 Yale University Protein capture membrane and method of use thereof
CN112354572B (en) * 2019-07-11 2022-02-11 北京理工大学 Multidimensional microfluidic electrophoresis chip, detection device and detection method
CN114636818B (en) * 2022-05-19 2022-07-29 天津德祥生物技术有限公司 Coating liquid, erythrocyte membrane coating liquid containing coating liquid and application of erythrocyte membrane coating liquid

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843657A (en) * 1994-03-01 1998-12-01 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6064754A (en) * 1996-11-29 2000-05-16 Oxford Glycosciences (Uk) Ltd. Computer-assisted methods and apparatus for identification and characterization of biomolecules in a biological sample
US6135942A (en) * 1998-11-30 2000-10-24 Leptin; Maria Nucleic acids proteins of a D. melanogaster insulin-like gene and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5438128A (en) * 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
JP2002535676A (en) * 1999-01-28 2002-10-22 ユニヴェルシテ ドゥ ジュネーブ Methods and kits for identifying or characterizing polypeptides

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843657A (en) * 1994-03-01 1998-12-01 The United States Of America As Represented By The Department Of Health And Human Services Isolation of cellular material under microscopic visualization
US6064754A (en) * 1996-11-29 2000-05-16 Oxford Glycosciences (Uk) Ltd. Computer-assisted methods and apparatus for identification and characterization of biomolecules in a biological sample
US5993627A (en) * 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6135942A (en) * 1998-11-30 2000-10-24 Leptin; Maria Nucleic acids proteins of a D. melanogaster insulin-like gene and uses thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1358314A4 *

Also Published As

Publication number Publication date
JP2004518949A (en) 2004-06-24
CA2428441A1 (en) 2002-06-20
AU2002243236B2 (en) 2007-11-08
EP1358314A4 (en) 2008-06-25
AU4323602A (en) 2002-06-24
EP1358314A2 (en) 2003-11-05
WO2002048674A2 (en) 2002-06-20

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