WO2000011956A1

WO2000011956A1 - Bacteriostatic and bactericidal compositions and method for utilization thereof

Info

Publication number: WO2000011956A1
Application number: PCT/JP1999/004642
Authority: WO
Inventors: Koji Nishikori
Original assignee: Bio Venture Bank Co., Ltd.
Priority date: 1998-08-27
Filing date: 1999-08-27
Publication date: 2000-03-09

Abstract

Bactericidal compositions comprising polylysine and a component extracted from citrus fruit seeds optionally together with a component extracted from citrus fruit peels. These bactericidal compositions can be safely added directly to foods. Also, they are safely usable in washing dishes, kitchen utensils, lavatories, etc.

Description

Description Bacteriostatic and disinfecting compositions and methods of use

The present invention relates to a novel bacteriostatic and bactericidal composition and a method for using the same, and more particularly to a bacteriostatic and bactericidal composition capable of exhibiting a bactericidal action by mixing substances having a bacteriostatic action. A composition that is not only safe to add directly to foods and drinks, but also safe for use in dishes, kitchen utensils, cleaning around toilets, and as an antibacterial coat. The present invention relates to a bacteriostatic and bactericidal composition and a method of using the same. Background art

Among bacteria, pathogenic microorganisms are the causative organisms that infect the human body and cause infectious diseases. As a route of transmission of such causative bacteria to the human body, there is usually a route of transmission from the human mouth via human hands, drinks, foods, and the like. In order to prevent bacterial infection from human hands, it is effective to wash hands thoroughly with water or stone before eating. To prevent bacterial infection from drinks and food, tap water and other drinking water are disinfected or boiled for drinking, and fresh food and other foods are fresh. When fresh food such as vegetables and seafood is eaten raw, it is common for the fresh food to be thoroughly washed before being served on the dining table, or when cooking and eating the food. Usually, heat treatment is used to kill pathogenic bacteria.

However, bacterial infection from human hands, beverages, food, etc. Unfortunately, it is not possible to completely prevent the transmission of pathogenic bacteria, even with the above and other conventional means.

Therefore, various methods for preventing infection by pathogenic bacteria have been used. One of the most effective ways to prevent the transmission of pathogenic bacteria is to kill the pathogenic bacteria. There are various methods for sterilizing pathogenic bacteria, such as sterilization by boiling, sterilization at high temperature and high pressure, sterilization with a bactericide, a chemotherapeutic agent, and the like. However, these sterilization methods are often not available depending on the object, and may not be applicable especially to those that come into direct contact with the human body, etc., or that enter the human body directly or indirectly through the mouth. . In addition, sterilization methods must be applied directly to the human body from the mouth, such as beverages and foods, or to the human body through the mouth or indirectly, such as washing. When applied to chemicals, detergents, etc., various restrictions are imposed. Most of the fungicides and chemotherapeutic agents used in sterilization methods using fungicides and chemotherapeutic agents can be used not only for beverages and foods but also for detergents and cleaning agents. prohibited. Therefore, germicides that can be used not only for drinks and foods but also for detergents and detergents are severely restricted, and the types of germicides must be extremely limited. It is.

On the other hand, especially when preserving food and drink such as beverages and food, not only sterilization but also the problem of decay must be considered. Even if foods and beverages are completely sterilized, even when completely preserved, foods and beverages can cause spoilage during the storage period, depending on the storage conditions and storage period. There is also.

The spoilage of foods and drinks is mainly caused by the growth of microorganisms such as spoilage bacteria present in them. In addition, microorganisms such as spoilage bacteria Proliferation largely depends on the temperature conditions in the preservation environment such as food and drink. The growth of microorganisms such as spoilage bacteria is usually promoted at normal or higher temperatures, so at low temperatures where the growth of those microorganisms is suppressed, the growth of the microorganisms is suppressed and the spoilage of foods and beverages is prevented. can do. However, there is a limit to the storage of food and drink at low temperatures.

Therefore, in order to prevent spoilage of foods and beverages, substances that suppress the growth of microorganisms such as spoilage bacteria have been developed and already commercialized. These substances are also called bacteriostats, bacteriostats, etc., do not have the action of killing pathogenic microorganisms or microorganisms such as spoilage bacteria, and even if they do, they are effective in killing bacteria. It does not have a bactericidal action as high as can be said, but has an action of suppressing the growth of such microorganisms.

As such a substance having a bacteriostatic and bacteriostatic action and being commercialized, a substance generally called polylysine is known. Polylysine has a well-established industrial production method, and its use as a food additive has been developed.

Polylysine is a naturally-occurring amino acid polymer, a polyamino acid in which the amino group at the £ -position and the carboxyl group of L-lysine, which is a type of essential amino acid, form a peptide bond and are linked linearly. It is. The amino acid constituting this f-polylysine is only L-lysine, and has a polymerization degree of 25 to 35 and a molecular weight of about 5,000. This ε-polylysine can be obtained by aerobically culturing a microorganism belonging to the genus Streptomyces (Jun Hiraki, J. Antibact. Antifung. Agents, Vol. 23, No. 6. Pp. 349-354, 1996).

Polylysine inhibits the growth of bacteria at a relatively low concentration against various bacteria, including Gram-positive and negative bacteria, and has a relatively high growth-inhibiting effect on yeast. In addition, polylysine has some T / J

Although it is said to have an inactivating effect, the inactivating effect is not recognized to the extent that it has an effect of killing bacteria.

On the other hand, citrus seed extract extracted from citrus seeds such as grapefruit has been approved as a food additive. It is said that this citrus seed extract can be added to a processed food to improve the preservability of the processed food, and suppress the bacteria possessed by the raw material of the processed food by immersing the raw material. . Such a citrus seed extract can be widely used as a natural antibacterial agent for general industrial use in general industrial applications, and is applied as antibacterial fiber, antibacterial paper and the like. However, it must be said that the antibacterial action of this citrus seed extract is not recognized as having a strong bactericidal activity that can be said to have a bactericidal action.

Therefore, it is a substance that can be used directly in foods and drinks, and is effective in preventing decay of foods and drinks to which it is applied, and has a bactericidal activity enough to prevent infection by pathogenic bacteria. Developing a substance is extremely useful from the viewpoint of preventing infection and from the viewpoint of food hygiene, and the development of such a substance has been long demanded. Disclosure of the invention

As a result of diligent research to meet such demands, the present inventors have found that, as a result of combining substances that have been found to have only bacteriostatic and bacteriostatic actions, it is extremely surprising that a certain combination of substances has an extremely high bactericidal action. The present inventors have found that a bacteriostatic and bactericidal composition capable of exhibiting the following can be obtained, and have completed the present invention.

Accordingly, an object of the present invention is to provide a novel bacteriostatic and bactericidal composition which has a high bactericidal action and is harmless to the human body and has high safety. In addition, the present invention can be directly used for food and drink, etc., and can be safely used for washing tableware, kitchen utensils, washing around toilets, cleaning, etc. Another object of the present invention is to provide a novel bacteriostatic and sterilizing composition having the following.

Further, the present invention provides a bacteriostatic and bactericidal composition comprising using such a novel safe and harmless bacteriostatic and bactericidal composition for preventing infection with pathogenic microorganisms. It is intended to provide a way to use.

Furthermore, the present invention relates to a novel bacteriostatic and bactericidal composition comprising the use of such a safe and harmless new bacteriostatic and bactericidal composition as a bactericide or a detergent or detergent. It is intended to provide a way to use the composition.

Furthermore, the present invention is characterized in that such a novel safe and harmless human bacterium and bactericidal composition is used as a bactericide or a cleaning agent or a detergent for the causative bacteria of food poisoning. It is another object of the present invention to provide a method for using a bacteriostatic and sterilizing composition. .

In addition, the present invention not only uses such a novel antibacterial and sterilizing composition directly in foods and drinks, but also for washing and cleaning tableware, kitchen utensils, toilets, and various other products. It is also an object of the present invention to provide a novel method for using a bacteriostatic and sterilizing composition that can be used safely as an antibacterial coat for instruments and tools.

In this specification, the bacteriostatic and bactericidal composition according to the present invention may be referred to simply as a bactericidal composition. It is to be understood that there is no intent to limit use to the disinfecting composition. In addition, the bacteriostatic and bacteriostatic effects of the bacteriostatic and bactericidal composition according to the present invention are described. In this case, it should be understood that the term bacteriostat includes not only bacteriostasis but also the effect of bacteriostasis, even if only bacteriostatic is described. Similarly, the term bacteriostatic may be referred to only, but the term bacteriostatic includes not only bacteriostatic but also bacteriostatic action and effect, and is not intended to be limited to that term. Can also be understood. Furthermore, it is to be understood that the term "fungi" or "bacterium" in the present invention includes not only bacteria such as causative bacteria causing food poisoning but also so-called pathogenic bacteria and viable bacteria.

In order to achieve the above object, the present invention provides a bacteriostatic and bactericidal composition comprising polylysine and a component extracted from a seed of a citrus plant fruit.

Further, the present invention provides an antibacterial and bactericidal composition for achieving the above object, further comprising an ingredient extracted from the skin of a citrus plant, thereby providing the composition extracted from the skin of the fruit of the citrus plant. The present invention provides a bacteriostatic and bactericidal composition having an effect such as deodorization.

The present invention further achieves the above-mentioned effects further by using grapefruit, summer tangerine, lemon, power pod, sudachi, tangerine, yokan, orange, mandarin, hassaku, navel, boncan, etc. as the citrus plant. And a bactericidal composition.

Furthermore, the effects of the bacteriostatic and disinfecting compositions provided by the present invention can be further achieved by using grapefruit as the citrus plant.

In addition, the present invention can achieve the above object by using the above-mentioned bacteriostatic and bactericidal composition for preventing infection with pathogenic microorganisms. The present invention also provides the use of the bacteriostatic and sterilizing composition as a bactericide or a detergent or a detergent. The above object can also be achieved by directly using it for food and drink or by using it for washing tableware, kitchen utensils and toilets. BEST MODE FOR CARRYING OUT THE INVENTION

The bacteriostatic and bactericidal composition according to the present invention comprises polylysine and a component extracted from seeds of citrus fruit.

As the polylysine that can be used in the present invention, any polylysine can be used as long as the lysine is a polyamino acid polymerized by a peptide bond. Particularly, it is an amino acid polymer found in nature, and Preferred is poly-lysine in which the amino group at the f-position and the carboxyl group of relysine are linearly linked by a peptide bond, and which can be used as a natural food preservative. It is a polylysine composed of lysine alone and can be used even in a polylysine preparation containing other components. Commercially available polylysine can also be used.For example, polylysine preparations containing polylysine and daricin as components, and polylysine preparations containing polylysine and acetic acid as main components can also be used. it can.

Examples of the citrus plant used in the present invention include grapefruit, summer tangerine, lemon, power boss, sudachi, tangerine, ginkgo, orange, mandarin, hatsaku, navel, and boncan, with grape roots being particularly preferred. As a natural component extracted from the seeds of the fruit of the citrus plant, an extract containing fatty acids and flavonoids as main components is preferable, and a natural component extracted from the seeds of the grapefruit fruit, and Particularly, an extract containing fatty acids and flavonoids as main components, which is a grade recognized as a natural food additive, is particularly preferable.

Natural extracted from seeds of citrus fruit used in the present invention The components can be extracted from the seeds of the fruit of the citrus plant by a conventional method. As a method for extracting such a natural component used in the present invention, for example, the natural component can be extracted by squeezing or solvent extraction. For example, in the squeezing method, an extract can be squeezed out from the fruit of a citrus plant using a general squeezing machine, and separated by a commercially available decanter or the like to extract the natural component. The extract obtained by solvent extraction is obtained by pulverizing citrus fruits using a pulverizer such as a ball mill or homogenizer, and then using a solvent that is harmless and safe to the human body, such as water and ethanol. They can be mixed and separated and manufactured by a method such as extraction and filtration. In addition, the added water and alcohol can be removed by evaporation or the like, and the solvent can be almost removed to obtain a concentrated extract, which can be used in the state of the concentrated extract.

As described above, the bacteriostatic and bactericidal composition according to the present invention comprises polylysine and a component extracted from the seed of a citrus fruit, and a component extracted from a citrus fruit peel. May be further included. Depending on the object to which the bacteriostatic and disinfecting composition according to the present invention is used, not only the purpose of disinfection but also the purpose of deodorization may be particularly important. In such a case, it is more effective to add an extract from the skin of the fruit of the citrus plant. The extract from the citrus fruit seeds described above has a high anti-tooth effect, but the citrus fragrance is weak, whereas the extract from the citrus fruit peel described above has an antibacterial effect. It is not very expensive, but has a high citrus aroma. Therefore, when it is desired to impart a citrus scent in addition to the antibacterial action, it is also preferable to use an extract from the skin of citrus plant seeds.

The extract from the skin of the citrus plant fruit is also obtained by extraction according to the conventional method described above in the same manner as in the case of obtaining the extract from the citrus plant seed. Can be

The bacteriostatic and bactericidal composition according to the present invention is a combination of components each having a bacteriostatic or bacteriostatic action, and is composed of a combination of components that can be used as a natural food preservative or an additive. Therefore, the bactericidal composition of the present invention has an extremely significant feature that it has not only a bacteriostatic or bacteriostatic action of each component but also a bactericidal action against various bacteria. In other words, the composition for sterilization according to the present invention exhibits a bactericidal action against microorganisms such as molds, as well as various bacteria whose components exhibit bacteriostatic action, for example, various bacteria including Gram-positive and Gram-negative. A particularly remarkable action and effect of the composition for sterilization according to the present invention is that it exhibits not only a bacteriostatic action but also a bactericidal action against various bacteria and molds that cause food poisoning. In other words, the composition for disinfection of the present invention can be used not only for bacteria causing food poisoning, such as Escherichia coli, Staphylococcus aureus, Salmonella, Vibrio parahaemolyticus, and cholera, but also for various other bacteria including Gram-positive and Gram-negative bacteria. Is also effective. Surprisingly, it is surprising that the disinfecting composition of the present invention effectively disinfects enterohemorrhagic Escherichia coli such as Escherichia coli O-157. Furthermore, the bacteriostatic and bactericidal compositions according to the present invention are also effective against molds such as Aspergillus niger and Aspergillus niger.

The bacteriostatic and bactericidal composition according to the present invention is essentially harmless to the human body and used for a long time because it is originally composed of components that can be used as natural food preservatives or additives. Therefore, it can be used in any field for the purpose of preventing infection from various bacteria including pathogenic microorganisms. Therefore, the bactericidal composition described above can be used not only for various kinds of utensils such as around toilets, but also for use in eating such as tableware and kitchen utensils. It can be used not only for various tools that come into direct or indirect contact, but also for foods and drinks that are directly ingested by the human mouth. The composition for disinfection according to the present invention can be used in various forms as a disinfectant, a detergent, or a detergent, and can be used in different forms depending on the object to be used. Further, the composition for sterilization according to the present invention can be widely used as a natural antibacterial agent for general industrial use in general industrial applications in general, in addition to the above-mentioned usage forms, and includes antibacterial fibers, antibacterial paper and the like. In addition to being applicable as an antibacterial coat, it can be used for various antibacterial treatments. Therefore, the composition for sterilization according to the present invention can be used for a wide range of applications such as antibacterial insoles, antibacterial filters, and antibacterial socks and antibacterial underwear. Can be used for various tools and equipment such as antibacterial baby tools, antibacterial toilet tools, and antibacterial handles.

When the composition for sterilization according to the present invention is directly used for foods and drinks, it is generally used in the form of a liquid such as a solution or a suspension. However, it can also be used in solid form. Further, when the above-mentioned disinfecting composition is used as a disinfectant or a cleaning agent or a cleaning agent, as in the above case, it may be used in the form of a liquid such as a solution or a suspension or a solid. Can also. For example, when the above disinfecting composition is used in the form of a disinfectant or a cleaning agent or a cleaning solution such as a hand, it is preferable to use the disinfecting composition in the form of a liquid such as an aqueous solution, and in the form of stone. If used, they can be used in liquid or solid form. In other words, the composition for sterilization according to the present invention can be used in an appropriate form according to the form of use such as food and drink, and can be used without any restrictions on the form of use. it can.

The amount of the polylysine in the composition for sterilization according to the present invention is also determined by The amount of the components extracted from the seeds of the fruit of the citrus plant also varies depending on the object, form, etc., of the composition for sterilization, and is not particularly limited. When used as a bactericide for foods and beverages, the amount of the polylysine is generally about 0.01 to 10% by weight, preferably 0 to 10% by weight, based on the bactericidal composition. 0.5 to 5% by weight, more preferably 0.1 to 1% by weight, and the amount of the components extracted from the seeds of the fruit of the citrus plant is as a concentrated extract in the composition for sterilization. in contrast, typically about 0.00 1-1 wt%, preferably from 0 · 00 5 to 0.5 wt ^_0/0, more preferably 0.0 1 to 0.1 wt%. Furthermore, the relative amounts of the polylysine and the components extracted from the seeds of the fruit of the citrus plant are not particularly limited, and can be appropriately changed depending on the use form, the use object, and the like.

When adding the components extracted from the skin of the fruit seeds of the citrus plant, it is preferable to appropriately change according to the degree of the citrus scent of the extracted components, and it is not particularly limited to a certain amount:

The composition for sterilization according to the present invention may further contain various additives depending on the purpose of use and the like. For example, when it is desired to adjust pH, p H can be adjusted appropriately, and when it is desirable to adjust the osmotic pressure, the osmotic pressure can be adjusted appropriately with an osmotic pressure adjusting agent.

The present invention will be described in more detail with reference to examples.

(Example 1)

The following components were weighed in predetermined amounts, mixed and diluted with a predetermined amount of water to obtain a composition for sterilization.

^ ^ Usage

3 g (0.3%) of polylysine (50% powder) P

12

Grapefruit seed extract

(Natural food additive) (Undiluted solution) 2 ml (500-fold dilution) Acetic acid 0.4 ml

The above components were mixed and diluted with water so that the total amount became 1 liter, to obtain a composition for sterilization.

0 fungus ^^)

The sterilizing composition having the above composition was tested for its antibacterial action against Escherichia coli, Staphylococcus aureus, Salmonella, and Vibrio, which are mainly the causative bacteria of food poisoning.

The bacteria used were Escherichia coli 0-157: H45 (a strain that does not produce Vero toxin) as Escherichia coli, Staphylococcus aureus (AO type toxin-producing strain) as Staphylococcus aureus, Salmonella enterotidis, and Salmonella as bacteria. Vibrio parahaenolyticus was used as the vibrio bacterium. Each bacterial suspension of bacteria were serially diluted, 1 0 ⁴ was Shun and test bacteria solution diluted to.

Each test bacterial solution (5 ml) and the above sterilizing composition (5 ml) were mixed, and allowed to stand in a constant temperature water bath at 20 ° C. After 5 minutes, 30 minutes, and 60 minutes after standing, 1 ml of the solution was placed in a plate, cultured on a standard agar medium at 37 ° C for 48 hours, and the number of bacteria was measured. As a control, a mixture of 5 ml of the test bacterial solution and 5 ml of the diluent was used in the same manner.

The results were as follows.

(1) Escherichia coli 0-157: H45

Action time vs. irradiation (/ m 1) specimen (m 1) - 0 minutes 7. 0 X 1 0 ⁴

5 minutes 4.7 XI 0 ⁴ 1 6 X 1 0 ²

30 minutes 5.4 xl 0 ⁴ 2 6 x 10 6 0 min 6.2 x 1 0 ⁴ 7

(2) Staphylococcus aureus

Action time vs. charge m 1) 0 minutes 1.5 X 10 ⁴

5 minutes 1.4 X 1 0 ⁴ 0

30 minutes 1.1 X 1 0 ⁴ 0

6 0 minutes 7.1 X 1 0 0

(3) Salmonella enterotidis;

Action time control (Zm 1) Sample_1 m 1)

0 min 2.4 X 1 0 ⁴

5 minutes 1.5 X 1 0 ⁴ 2 5 x 1 0 ² 3 0 minutes 2.8 X 1 0 ⁴ 1 4 1 0 6 0 minutes 4.0 X 1 0 ⁴ 0

(4) Vibrio parahaenolyticus

Action time control (Zm 1) Material m 1)

0 min 3.6 X 1 0 ⁴

5 minutes 3.4 X 1 0 ⁴ 0

3 0 min 3.9 X 1 0 ⁴ 0

60 minutes 4.1 X 10 ⁴⁰⁰ Further, the bactericidal action of the composition according to the present invention was examined. This bactericidal action was carried out by diluting the solution of the composition with water. The results are shown in Table 1.

table 1 Test bacteria dilution result (C FU / m 1)

(Times) 0 hour 30 minutes 1 hour 24 hours Listeria monocytogenes 0 6 X 10 ⁵ 3 X 10 ⁵ 8 X 10 ⁵ 2 X 10 ⁶

(Listeria 2 0 6 X 1 0 ⁵ 0 0 0 monocyto5 0 6 1 0 ^s 0 0 0 genes) 1 0 0 6 X 1 0 ⁵ 0 0 0

2 0 0 6 X 1 0 ⁵ 2 0 3 0 0 E. coli 0 4 X 1 0 ⁵ 8 X 1 0 9 X 1 0 ³ 2 X 1 0 ⁴

(E. coli 204 X 1 0 ⁵ 0 0 0 0-157: H7) 5 0 4 1 0 ⁵ 0 0 0

1 0 0 4 X 1 0 ⁵ 1 0 0 0 2 0 0 4 X 1 0 ⁵ 3 0 5 0 8 0 Sazolemmonella 0 8 X 1 0 ⁵ 9 X 1 0 ⁵ 6 X 1 0 ⁵ 3 X 1 0 ⁵

(Salmonella 2 0 8 X 1 0 ⁵ 0 0 0 typhimurium) 5 0 8 X 1 0 ⁵ 0 0 0

1 0 0 8 X 1 0 ⁵ 5 0 2 0 2 0 2 0 0 8 X 1 0 ⁵ 6 0 1 0 0

: 3 germs 0 9 X 10 ⁴ 1 X 10 ⁵ 9 X 10 ⁴ 3 X 10 ⁴

(Vibrio 2 0 9 X 1 0 0 0 0 cholerae) 5 0 9 X 1 0 ⁴ 0 0 0

1 0 0 9 X 1 0 ⁴ 0 0 0 2 0 0 9 X 1 0 5 0 4 0 0 Vibrio parahaemolyticus 0 7 X 1 0 ⁴ 7 X 10 ⁴ 3 X 1 0 ⁴ 1 X 10 ⁴ bacteria (Vibrio 2 0 7 X 1 0 ⁴ 0 0 0 parahaemoly- 5 0 7 X 1 0 ⁴ 0 0 0 ticus 1 0 0 7 X 1 0 ⁴ 0 0 0

2 0 0 7 X 1 0 ⁴ 0 1 0 0 Yellow butterfly 0 6 X 1 0 ⁵ 3 X 1 0 ⁵ 2 X 1 0 ⁵ 8 X 1 0 ⁵

2 0 6 X 1 0 ⁵ 0 0 0

(Staphylo5 0 6 X 1 0 ⁵ 0 0 0 coccus 1 0 0 6 X 1 0 ⁵ 7 0 7 0 3 0 aureus) 2 0 0 6 X 1 0 ⁵ 1 2 0 1 5 0 1 0 0

From the results shown in Table 1 above, the composition according to the present invention, at a predetermined dilution concentration, is effective against bacteria such as Listeria monocytogenes, pathogenic Escherichia coli, Salmonella, Vibrio parahaemolyticus, Vibrio parahaemolyticus, and Staphylococcus aureus. It was shown to have a bactericidal effect.

Still further, the composition according to the present invention may be sterilized in saline. The effect was investigated. This bactericidal action was performed by a dilution method using a solution of the above composition in saline (sterilized water containing 3% synthetic salt). The results are shown in Table 2.

Table 2

From the results shown in Table 2 above, it was found that the composition according to the present invention exhibited a bactericidal action against bacteria such as Escherichia coli, cholera and Vibrio parahaemolyticus even in seawater at a certain predetermined dilution concentration.

In addition to these bactericidal actions, the composition according to the present invention was examined for its antifungal action against mold. To determine the antifungal activity of this composition, the minimum inhibitory concentration (MIC) against mold was determined. Using the Aspergillus fungus (A spergillusniger FE RM S-1) and Aspergillus niger (CI adosporiumc 1 adosporioides FE RM S-8) as the test mold, the above composition (20-fold concentrated solution) was specified. Dilute to the desired dilution and use the MIC measurement method (242L agar method) Measured its MIC. That is, the above composition (20-fold concentrated solution) is diluted to a predetermined dilution, and the diluted solution is inoculated on a potato dextrorose agar medium and cultured at a temperature of 25 ° C ± 1 ° C for 5 days. Therefore, the gel that showed clear inhibition was judged as MIC. The results are shown in Table 3 (Aspergillus niger) and Table 4 (Aspergillus niger).

Table 3 (Aspergillus niger)

From the results in Tables 3 and 4, it was found that the minimum growth inhibitory concentration (MIC) of the composition according to the present invention against black mold and mildew was 1Z 8-fold and 1 / 2-fold diluted concentrations, respectively. did.

From these bactericidal and antifungal activities, it was found that the composition according to the present invention was effective not only against various bacteria but also against viper. Industrial applicability

As described above, the composition for sterilization according to the present invention comprises polylysine and components extracted from seeds of fruits of citrus plants, and all of these components are recognized as food additives. It has the great advantage of being harmless and safe for the human body as well as harmless and safe even if it enters the human body through the mouth.

Further, another extremely great advantage is that the composition for sterilization according to the present invention has a bactericidal effect on various bacteria which are particularly problematic as the causative bacteria of food poisoning. In addition to this, the composition according to the present invention has an extremely great advantage that it has an antifungal action against mold that is difficult to control.

Further, the composition for sterilization according to the present invention has an advantage that it is only necessary to simply mix the above-mentioned components, and the production is extremely simple.

Claims

The scope of the claims

1. A bacteriostatic and fungicidal composition comprising polylysine and a component extracted from the seed of a citrus fruit.

2. The bacteriostatic and fungicidal composition according to claim 1, further comprising an ingredient extracted from the skin of a citrus fruit. .

3. The bacteriostatic and bactericidal composition according to claim 1 or 2, wherein the citrus plant is grapefruit, summer tangerine, lemon, power pod, sudachi, tangerine, ginkgo, orange, mandarin. A bacteriostatic and bactericidal composition, which is selected from the group consisting of: honeysuck, navel and bonkan.

4. The bacteriostatic and bactericidal composition according to any one of claims 1 to 3, wherein the citrus plant is grapefruit. Composition.

5. A bacteriostat, characterized in that the bacteriostat and the composition for sterilization according to any one of claims 1 or 4 are used for preventing infection with a pathogenic microorganism. How to use the composition for sterilization.

6. The method of using the bacteriostatic and bactericidal composition according to claim 5, wherein the bacteriostatic and bactericidal composition further comprises a bactericide or a detergent. Or a method for using the composition for bacteriostatic and disinfecting purposes, which is used as a detergent.

7. The method of using the bacteriostatic and bactericidal composition according to claim 5 or 6, wherein the bacteriostatic and bactericidal composition is treated with a bactericide, a cleaning agent, or a cleaning agent against the causative bacteria of food poisoning. A method for using a composition for bacteriostatic and disinfecting, which is used as an agent.