SU780444A1 - Method of obtaining desohyribonucleic acid - Google Patents
Method of obtaining desohyribonucleic acid Download PDFInfo
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- SU780444A1 SU780444A1 SU792784850A SU2784850A SU780444A1 SU 780444 A1 SU780444 A1 SU 780444A1 SU 792784850 A SU792784850 A SU 792784850A SU 2784850 A SU2784850 A SU 2784850A SU 780444 A1 SU780444 A1 SU 780444A1
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- SU
- USSR - Soviet Union
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- acid
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- protein
- deproteinization
- desohyribonucleic
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Abstract
СПОСОБ ПОЛУЧЕНИЯ ДЕЗОКСИРИБОНУКЛЕИНОВОЙ КИСЛОТЬ .из молок рыб путем их гомогенизации с последующим промыванием гомогената физиологическим liacTBOpoM и депротеинизацией выделенного комплекса ДНК с белком, отличающийс тем, что, с целью повышени степени чистоты коночного продукта и удешевлени способа, депротеинизацию комплекса ДНК с белком ведут препаратом протосубтилин , вьщеленным из Bacillus Zubtilis.METHOD OF OBTAINING DEOXYRIBONUCKLEIC ACID. From fish milk by homogenization followed by washing the homogenate with physiological liacTBOpoM and deproteinization of the isolated DNA complex with a protein, characterized in that, in order to increase the purity of the final product and reduce the cost of the deprotement, it will be necessary to deproteinate. from Bacillus Zubtilis.
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4 4 1 7 Изобретение относитс к области органической химии, более тсчно к химии физиологически активных спединений . Известен способ получени дезокси рибонуклеиновой кислоты из молок рыб путем их гомогенизации споследующим промыванием гомогената физиологическим раствором и депротеинизацией выделенного комплекса ДНК с белком ij Однако известный способ не обл-,да ет высокой степенью, чистоты конечног продукта и вл етс очень дорогим. Цель изобретени повышение степени чистоты конечного продукта и удешевление способа. ---..-:,-,,:,,, Цель достигаетс тем, что депротеинизацию комплекса ДНК с белком ведут препаратом протосубтилин, выделенным из Bacillus Zubtilis. Пример. 1 кг замороженных молок лосос пропускают через м сорубку , затем гомогенизируют в гомогенизаторе РТ-2 (2-3 мин.V 8000 об./мин), добавл ют равный объем 0,13 М NaC1. Гомогенат ueirrpH-фугирукл- при ЗООП q в печение 5 мкн., супериатамт отбрасывают, осадок триж .ды промывают О,15 М NaCl (порпинми по I л), затем садпк ДНИ дважды пpo ывgют этанолом (порци ми rto 1 л) . Далее ДНП суспендируют в 0,25 М NaCl - 0,03 М NnHCOj гП 9,0 (кс1нпентраии 2 мг/мл), нагревают смесь до 35 С, добавл ют протосубтилин до 1,2 ед. акт./м.п. и выдерживают 30 мин при слабом перемешивании. З.атем пентрифугируют при 10000 q в течение 30 мин. Недостаточный слой смешивают с равным объемом этанола, пр ди ДНК извлекают из смеси, промывают этанолом и высушивают. Выход препарата 600000 о.е. 2607кг, содержание ДНК 68% . РНК - 1,6%, белка - 2,4%. Предложенный способ позвол ет получить конечный продукт, обладающий высокой степенью чистоты и удешевить процесс, поскольку используетс Протосубтилин.4 4 1 7 The invention relates to the field of organic chemistry, more specifically to the chemistry of physiologically active compounds. A known method for producing deoxy ribonucleic acid from fish milk by homogenization followed by washing the homogenate with physiological saline and deproteinization of the isolated DNA complex with protein ij. However, the known method does not have a high degree of purity of the final product and is very expensive. The purpose of the invention is to increase the purity of the final product and reduce the cost of the method. ---..-:, - ,,: ,, ,, The goal is achieved by the fact that the deproteinization of the DNA complex with the protein is carried out by the preparation of protosubtilin isolated from Bacillus Zubtilis. Example. 1 kg of frozen salmon milks are passed through a miter, then homogenized in a PT-2 homogenizer (2-3 min. V 8000 rpm), an equal volume of 0.13 M NaC1 is added. The homogenate ueirrpH-fugirukl- at ZOOP q in the oven 5 microns., The superamide is discarded, the precipitate is washed three times with O, 15 M NaCl (porphyrin according to I l), then the garden filaments are digested twice with ethanol (portions rto 1 l). Next, the DNPs are suspended in 0.25 M NaCl-0.03 M NnHCOj hP 9.0 (2 mg / ml concentration), the mixture is heated to 35 ° C, and the protosubtilin is added to 1.2 units. act / mp and incubated for 30 minutes with weak stirring. Z. then pentrifuge at 10,000 q for 30 min. The insufficient layer is mixed with an equal volume of ethanol, and the DNA is removed from the mixture, washed with ethanol and dried. The output of the drug 600000 OE 2607kg, the content of DNA is 68%. RNA - 1.6%, protein - 2.4%. The proposed method allows to obtain a high purity end product and reduce the cost of the process, since Protosubtilin is used.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792784850A SU780444A1 (en) | 1979-06-25 | 1979-06-25 | Method of obtaining desohyribonucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU792784850A SU780444A1 (en) | 1979-06-25 | 1979-06-25 | Method of obtaining desohyribonucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU780444A1 true SU780444A1 (en) | 1985-04-23 |
Family
ID=20835762
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU792784850A SU780444A1 (en) | 1979-06-25 | 1979-06-25 | Method of obtaining desohyribonucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU780444A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994004551A1 (en) * | 1992-08-26 | 1994-03-03 | Jury Petrovich Vainberg | Method of obtaining sodium salt of native dna |
| US5646127A (en) * | 1991-12-09 | 1997-07-08 | Crinos Industria Farmacobiologica S.P.A. | Treatment of cardiac ischemia by administration of a fraction of partially depolymerized DNA |
-
1979
- 1979-06-25 SU SU792784850A patent/SU780444A1/en active
Non-Patent Citations (1)
| Title |
|---|
| 1. Прикладна биохими и микробиологи , т. 14 № 4 с 602 1978. * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5646127A (en) * | 1991-12-09 | 1997-07-08 | Crinos Industria Farmacobiologica S.P.A. | Treatment of cardiac ischemia by administration of a fraction of partially depolymerized DNA |
| US5646268A (en) * | 1991-12-09 | 1997-07-08 | Crinos Industria Farmacobiologica S.P.A. | Process producing lower molecular weight range oligodeoxyribonucleotides |
| US6046172A (en) * | 1991-12-09 | 2000-04-04 | Crinos Industria Farmacobiologica Spa | Hydrolytically processed oligodeoxyribonucleotides and their pharmaceutical compositions |
| WO1994004551A1 (en) * | 1992-08-26 | 1994-03-03 | Jury Petrovich Vainberg | Method of obtaining sodium salt of native dna |
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