SU303886A1 - METHOD OF OBTAINING A LIVING GRIPPOSOUS VACCINE - Google Patents
METHOD OF OBTAINING A LIVING GRIPPOSOUS VACCINEInfo
- Publication number
- SU303886A1 SU303886A1 SU1357184A SU1357184A SU303886A1 SU 303886 A1 SU303886 A1 SU 303886A1 SU 1357184 A SU1357184 A SU 1357184A SU 1357184 A SU1357184 A SU 1357184A SU 303886 A1 SU303886 A1 SU 303886A1
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- vaccine
- obtaining
- gripposous
- living
- virus
- Prior art date
Links
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Description
Изобретение относитс к области медицины, точнее к методам получени профилактических вакцин, и касаетс способов получени живой гриппозной вакцины.The invention relates to the field of medicine, more specifically to methods for producing prophylactic vaccines, and relates to methods for producing a live influenza vaccine.
Известный способ нолучеии живой гриппозной вакцины, основанный на приготовлении вируссодержащей суспензии путем внесени в среду культивировани , в качестве которой используетс аллантоисна жидкость 9-10-дневных куриных эмбрионов, вирусов А2 и В, сравнительно сложен.The known method of obtaining live influenza vaccine, based on the preparation of a virus-containing suspension by introducing it into a culture medium, which uses allantoic liquid of 9-10 days old chicken embryos, A2 and B viruses, is relatively complex.
Цель насто щего изобретени - упроп1,епие процесса получени живой гриппозной вакцины-достигаетс приготовлением вируссодержащей суспепзии из тканевой культуры почек 19-дневных куриных эмбрионов.The purpose of the present invention is to support the process of obtaining a live flu vaccine, which is achieved by preparing a virus-containing suspension from kidney tissue culture of 19-day-old chicken embryos.
Дл нолучени живой гриппозпой вакцины по предлагаемому способу извлеченные почки 19-дневных куриных эмбрионов измельчают, например обрабатыва трипсином или механическим способом, полученную взвесь перенос т в питательную среду, куда одновременно внос т вирусы гриппа типа А и В в колнчестве 10«-10 и ИДбо па 1 л со ответственно.In order to obtain a live influenza vaccine using the proposed method, the extracted kidneys of 19-day-old chicken embryos are crushed, for example, treated with trypsin or mechanically, the resulting suspension is transferred to a nutrient medium, where viruses of type A and B are simultaneously administered in 10 -10 and ID Pa 1 l accordingly.
Средо дл культивировани вл етс синтетическа среда 199 с мономипииом (100 ед/мл) п нистатином (15 ед/мл). Дл вируса гриппа тнпа В в среду дополнительно ввод т 2-5% нрогретой сыворотки крупного рогатого скота.The culture medium is synthetic medium 199 with monomipium (100 U / ml) p nystatin (15 U / ml). For influenza A virus, an additional 2–5% of heated cattle serum is added to the medium.
Культивирование ведут при перемешнван1П1 и температуре 37°С в течение 48-72 час дл вируса гриппа тппа АЗ и 96-120 час дл вируса гриппа типа В.Cultivation is carried out at vmeshnivan1P1 and a temperature of 37 ° C for 48-72 hours for influenza virus TPA AZ and 96-120 hours for influenza virus type B.
Предмет изобретени Subject invention
Способ получепи живой гриппозной вакципы , основанный на прпготовлении вируссодержащей суспензии путем внесени в среду культивировани вируса гриппа штамма Ач и В, отличающийс тем, что, с целью упрощепн процесса, вируссодержащую суспепзию готов т из тканевой культуры почек 19-дневных куриных эмбрионов.The method of obtaining live influenza vaccine, based on the preparation of a virus-containing suspension by introducing the Ah and B strain into the influenza virus cultivation medium, characterized in that, in order to simplify the process, the virus-containing suspension is prepared from tissue culture of the kidneys of 19-day-old chicken embryos.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1357184A SU303886A1 (en) | 1969-08-15 | 1969-08-15 | METHOD OF OBTAINING A LIVING GRIPPOSOUS VACCINE |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SU1357184A SU303886A1 (en) | 1969-08-15 | 1969-08-15 | METHOD OF OBTAINING A LIVING GRIPPOSOUS VACCINE |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU303886A1 true SU303886A1 (en) | 1973-10-19 |
Family
ID=20447117
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1357184A SU303886A1 (en) | 1969-08-15 | 1969-08-15 | METHOD OF OBTAINING A LIVING GRIPPOSOUS VACCINE |
Country Status (1)
| Country | Link |
|---|---|
| SU (1) | SU303886A1 (en) |
-
1969
- 1969-08-15 SU SU1357184A patent/SU303886A1/en active
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