Nothing Special   »   [go: up one dir, main page]

SG11202012785VA - Crispr double nickase based amplification compositions, systems, and methods - Google Patents

Crispr double nickase based amplification compositions, systems, and methods

Info

Publication number
SG11202012785VA
SG11202012785VA SG11202012785VA SG11202012785VA SG11202012785VA SG 11202012785V A SG11202012785V A SG 11202012785VA SG 11202012785V A SG11202012785V A SG 11202012785VA SG 11202012785V A SG11202012785V A SG 11202012785VA SG 11202012785V A SG11202012785V A SG 11202012785VA
Authority
SG
Singapore
Prior art keywords
systems
methods
based amplification
double nickase
amplification compositions
Prior art date
Application number
SG11202012785VA
Other languages
English (en)
Inventor
Feng Zhang
Max Kellner
Jonathan Gootenberg
Omar Abudayyeh
Original Assignee
Broad Inst Inc
Massachusetts Inst Technology
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broad Inst Inc, Massachusetts Inst Technology, Harvard College filed Critical Broad Inst Inc
Publication of SG11202012785VA publication Critical patent/SG11202012785VA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/20Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/101Temperature
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/125Specific component of sample, medium or buffer
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202012785VA 2018-06-26 2019-06-26 Crispr double nickase based amplification compositions, systems, and methods SG11202012785VA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862690278P 2018-06-26 2018-06-26
US201862767059P 2018-11-14 2018-11-14
PCT/US2019/039221 WO2020006067A1 (en) 2018-06-26 2019-06-26 Crispr double nickase based amplification compositions, systems, and methods

Publications (1)

Publication Number Publication Date
SG11202012785VA true SG11202012785VA (en) 2021-01-28

Family

ID=67297327

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202012785VA SG11202012785VA (en) 2018-06-26 2019-06-26 Crispr double nickase based amplification compositions, systems, and methods

Country Status (12)

Country Link
US (1) US20210207203A1 (es)
EP (1) EP3814520A1 (es)
JP (1) JP2021528091A (es)
KR (1) KR20210024010A (es)
CN (1) CN112639121A (es)
AU (1) AU2019291827A1 (es)
BR (1) BR112020026246A2 (es)
CA (1) CA3102211A1 (es)
IL (1) IL278963A (es)
MX (1) MX2020013461A (es)
SG (1) SG11202012785VA (es)
WO (1) WO2020006067A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230272456A1 (en) * 2020-06-03 2023-08-31 Siphox, Inc. Cascading amplification for chemical and biosensing
CN111733216B (zh) * 2020-06-22 2023-03-28 山东舜丰生物科技有限公司 一种提高目标核酸检测效率的方法
JP7539136B2 (ja) 2020-08-05 2024-08-23 国立大学法人 長崎大学 ウイルスベクターによるcas9遺伝子の部位特異的導入方法
CN112831544B (zh) * 2020-12-31 2024-06-14 华南农业大学 一种基于CRISPR/Cas12a系统的生物检测方法和生物检测装置
CN113186253B (zh) * 2021-04-27 2022-06-21 福州大学 一种用于检测路易体病标志物的Cas12a-DNAzyme传感器及其制备方法
WO2023004391A2 (en) 2021-07-21 2023-01-26 Montana State University Nucleic acid detection using type iii crispr complex
WO2024187091A1 (en) * 2023-03-08 2024-09-12 Seek Labs, Inc. Compositions and methods of isothermal nucleic acid amplification

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US715640A (en) 1902-09-10 1902-12-09 Whitney Mfg Company Clutch mechanism.
US5541099A (en) 1989-08-10 1996-07-30 Life Technologies, Inc. Cloning and expression of T5 DNA polymerase reduced in 3'-to-5' exonuclease activity
US6555349B1 (en) 1993-01-22 2003-04-29 Cornell Research Foundation, Inc. Methods for amplifying and sequencing nucleic acid molecules using a three component polymerase
CN100342008C (zh) 1997-10-24 2007-10-10 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
CN102939377B (zh) 2010-04-26 2016-06-08 桑格摩生物科学股份有限公司 使用锌指核酸酶对Rosa位点进行基因组编辑
KR102362649B1 (ko) * 2012-04-09 2022-02-14 엔바이로로직스 인크. 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
JP2016501531A (ja) 2012-12-12 2016-01-21 ザ・ブロード・インスティテュート・インコーポレイテッド 配列操作および治療適用のための系、方法および組成物の送達、エンジニアリングおよび最適化
WO2014164479A1 (en) * 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
KR20160034901A (ko) 2013-06-17 2016-03-30 더 브로드 인스티튜트, 인코퍼레이티드 서열 조작에 최적화된 crispr-cas 이중 닉카아제 시스템, 방법 및 조성물
EP3183358B1 (en) * 2014-08-19 2020-10-07 President and Fellows of Harvard College Rna-guided systems for probing and mapping of nucleic acids
US10577649B2 (en) * 2014-11-11 2020-03-03 Illumina, Inc. Polynucleotide amplification using CRISPR-Cas systems
EP3271713B1 (en) 2015-03-18 2021-05-05 The Broad Institute, Inc. Massively parallel on-chip coalescence of microemulsions
AU2016279062A1 (en) * 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
EP3430134B1 (en) 2015-06-18 2022-09-21 The Broad Institute, Inc. Novel crispr enzymes and systems
CN116814590A (zh) 2015-10-22 2023-09-29 布罗德研究所有限公司 Vi-b型crispr酶和系统
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
JP6914274B2 (ja) 2016-01-22 2021-08-04 ザ・ブロード・インスティテュート・インコーポレイテッド Crisprcpf1の結晶構造
CA3026055A1 (en) 2016-04-19 2017-10-26 The Broad Institute, Inc. Novel crispr enzymes and systems
AU2017257274B2 (en) * 2016-04-19 2023-07-13 Massachusetts Institute Of Technology Novel CRISPR enzymes and systems
US11286478B2 (en) 2016-04-19 2022-03-29 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
EP4368637A3 (en) * 2016-05-20 2024-07-10 Regeneron Pharmaceuticals, Inc. Methods for breaking immunological tolerance using multiple guide rnas
AU2017283713B2 (en) 2016-06-17 2021-04-08 Massachusetts Institute Of Technology Type VI CRISPR orthologs and systems
CN110312799A (zh) 2016-08-17 2019-10-08 博德研究所 新型crispr酶和系统
WO2018035388A1 (en) 2016-08-17 2018-02-22 The Broad Institute, Inc. Novel crispr enzymes and systems
SI3551753T1 (sl) 2016-12-09 2022-09-30 The Broad Institute, Inc. Diagnostika, temelječa na sistemu CRISPR EFFECTOR
WO2018170333A1 (en) 2017-03-15 2018-09-20 The Broad Institute, Inc. Novel cas13b orthologues crispr enzymes and systems
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11104937B2 (en) 2017-03-15 2021-08-31 The Broad Institute, Inc. CRISPR effector system based diagnostics
WO2018170340A1 (en) 2017-03-15 2018-09-20 The Broad Institute, Inc. Crispr effector system based diagnostics for virus detection
US11618928B2 (en) 2017-04-12 2023-04-04 The Broad Institute, Inc. CRISPR effector system based diagnostics for malaria detection
BR112019021378A2 (pt) 2017-04-12 2020-05-05 Massachusetts Inst Technology ortólogos de crispr tipo vi inovadores e sistemas
WO2018194963A1 (en) 2017-04-16 2018-10-25 Sanford Health Filter for stent retriever and methods for use thereof
KR102678809B1 (ko) 2017-05-18 2024-06-26 더 브로드 인스티튜트, 인코퍼레이티드 표적화된 핵산 편집을 위한 시스템, 방법 및 조성물
WO2019005866A1 (en) 2017-06-26 2019-01-03 The Broad Institute, Inc. NEW TYPE VI CRISPR ORTHOLOGISTS AND ASSOCIATED SYSTEMS
CN109209763B (zh) 2017-07-06 2019-11-29 北京金风科创风电设备有限公司 风力发电机组叶片变桨装置、变桨方法及风力发电机组
CN112501254B (zh) * 2017-07-14 2024-07-19 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒

Also Published As

Publication number Publication date
AU2019291827A1 (en) 2020-12-24
CN112639121A (zh) 2021-04-09
CA3102211A1 (en) 2020-01-02
BR112020026246A2 (pt) 2021-04-20
KR20210024010A (ko) 2021-03-04
MX2020013461A (es) 2021-04-28
US20210207203A1 (en) 2021-07-08
WO2020006067A1 (en) 2020-01-02
EP3814520A1 (en) 2021-05-05
JP2021528091A (ja) 2021-10-21
IL278963A (en) 2021-01-31

Similar Documents

Publication Publication Date Title
IL278963A (en) Dual NICKASE-based CRISPR amplification preparations, systems and methods
IL281159A (en) New CRISPR enzymes and systems
IL285215A (en) Training modulators, preparations and their methods
EP3645054A4 (en) COMPOSITIONS BASED ON CRISPR / CAS-ADENIN-DEAMINASE, SYSTEMS AND METHODS FOR TARGETED NUCLEIC ACID EDITING
IL272089A (en) Compounds, preparations and methods
EP3655530A4 (en) NEW TYPE VI CRISPR ORTHOLOGIST AND SYSTEMS
EP3500677A4 (en) UNIQUE GUIDE RNA, CRISPR / CAS9 SYSTEMS AND METHODS OF USE
EP3676297A4 (en) COMPOUNDS, COMPOSITIONS AND PROCESSES
EP3715461A4 (en) GENOMIC EDITION COMPOSITION USING A CRISPR / CPF1 SYSTEM AND ITS USE
EP3728577A4 (en) PLANT GENE EDITING SYSTEMS, PROCEDURES AND COMPOSITIONS
IL285302A (en) Compounds, preparations and methods
EP3884065A4 (en) DIRECT-TO-LIBRARY METHODS, SYSTEMS AND COMPOSITIONS
EP3601538A4 (en) ANK AND IL-12 COMPOSITIONS AND METHODS
SG11202011240YA (en) Crispr/cas and transposase based amplification compositions, systems and methods
GB201901099D0 (en) Methods, uses and compositions
IL279059A (en) Methods and preparations for killing spores
GB202017595D0 (en) Compositions, and methods and uses relating thereto
GB2576614B (en) Compositions, uses and methods
IL287120A (en) Compounds, preparations and methods
GB201804163D0 (en) Uses, compositions and methods
EP3720281A4 (en) PESTICIDE COMPOSITIONS AND PROCEDURES
EP3307336A4 (en) Biomaterial compositions, implants, and methods of making the same
EP3596082A4 (en) CDPK1 INHIBITORS, ASSOCIATED COMPOSITIONS AND PROCESSES
EP3612230A4 (en) PALBOCICLIB COMPOSITIONS AND RELATED METHODS
HUP1500219A1 (hu) Alginit hidrolizátumot és gilisztahumusz hidrolizátumot tartalmazó talaj/vagy növénykondicionáló készítmények