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MX2020013461A - Composiciones, sistemas y métodos de amplificación basados en la doble nicasa crispr. - Google Patents

Composiciones, sistemas y métodos de amplificación basados en la doble nicasa crispr.

Info

Publication number
MX2020013461A
MX2020013461A MX2020013461A MX2020013461A MX2020013461A MX 2020013461 A MX2020013461 A MX 2020013461A MX 2020013461 A MX2020013461 A MX 2020013461A MX 2020013461 A MX2020013461 A MX 2020013461A MX 2020013461 A MX2020013461 A MX 2020013461A
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MX
Mexico
Prior art keywords
systems
detection
crispr
embodiments disclosed
methods
Prior art date
Application number
MX2020013461A
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English (en)
Inventor
Feng Zhang
Max Kellner
Jonathan Gootenberg
Omar Abudayyeh
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Broad Inst Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broad Inst Inc filed Critical Broad Inst Inc
Publication of MX2020013461A publication Critical patent/MX2020013461A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Las modalidades descritas en el presente documento utilizan efectores de orientación de ARN para proporcionar métodos y sistemas robustos de amplificación de ácidos nucleicos basados en CRISPR. Las modalidades aquí descritas pueden amplificar tanto ácidos nucleicos diana bicatenarios como monocatenarios. Además, las modalidades descritas en la presente, pueden combinarse con diversas plataformas de detección, por ejemplo, CRISPR-SHERLOCK, para lograr la detección y el diagnóstico con sensibilidad attomolar. Dichas modalidades son útiles en múltiples escenarios de la salud humana, lo que incluye, por ejemplo, la detección viral, la tipificación de cepas bacterianas, el genotipado sensible y la detección de ADN libre de células asociado a enfermedades.
MX2020013461A 2018-06-26 2019-06-26 Composiciones, sistemas y métodos de amplificación basados en la doble nicasa crispr. MX2020013461A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862690278P 2018-06-26 2018-06-26
US201862767059P 2018-11-14 2018-11-14
PCT/US2019/039221 WO2020006067A1 (en) 2018-06-26 2019-06-26 Crispr double nickase based amplification compositions, systems, and methods

Publications (1)

Publication Number Publication Date
MX2020013461A true MX2020013461A (es) 2021-04-28

Family

ID=67297327

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020013461A MX2020013461A (es) 2018-06-26 2019-06-26 Composiciones, sistemas y métodos de amplificación basados en la doble nicasa crispr.

Country Status (12)

Country Link
US (1) US20210207203A1 (es)
EP (1) EP3814520A1 (es)
JP (1) JP2021528091A (es)
KR (1) KR20210024010A (es)
CN (1) CN112639121A (es)
AU (1) AU2019291827A1 (es)
BR (1) BR112020026246A2 (es)
CA (1) CA3102211A1 (es)
IL (1) IL278963A (es)
MX (1) MX2020013461A (es)
SG (1) SG11202012785VA (es)
WO (1) WO2020006067A1 (es)

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JP7539136B2 (ja) 2020-08-05 2024-08-23 国立大学法人 長崎大学 ウイルスベクターによるｃａｓ９遺伝子の部位特異的導入方法
CN112831544B (zh) * 2020-12-31 2024-06-14 华南农业大学 一种基于CRISPR/Cas12a系统的生物检测方法和生物检测装置
CN113186253B (zh) * 2021-04-27 2022-06-21 福州大学 一种用于检测路易体病标志物的Cas12a-DNAzyme传感器及其制备方法
WO2023004391A2 (en) 2021-07-21 2023-01-26 Montana State University Nucleic acid detection using type iii crispr complex
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Also Published As

Publication number Publication date
AU2019291827A1 (en) 2020-12-24
CN112639121A (zh) 2021-04-09
CA3102211A1 (en) 2020-01-02
BR112020026246A2 (pt) 2021-04-20
KR20210024010A (ko) 2021-03-04
SG11202012785VA (en) 2021-01-28
US20210207203A1 (en) 2021-07-08
WO2020006067A1 (en) 2020-01-02
EP3814520A1 (en) 2021-05-05
JP2021528091A (ja) 2021-10-21
IL278963A (en) 2021-01-31

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