RU97100857A

RU97100857A - SIMULTANEOUS DEFINITION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXONS WITH THE HELP OF HYBRID ANALYSIS

Info

Publication number: RU97100857A
Application number: RU97100857/13A
Authority: RU
Inventors: Герт Яннес; Руди Россо; Хеверсвин Хюго Ван
Original assignee: Иннодженетикс Н.В.
Priority date: 1994-06-24
Filing date: 1995-06-23
Publication date: 1999-02-27

Claims

1. The method of determining and identifying at least one microorganism or the simultaneous determination of several microorganisms in a sample, comprising the steps of
(i) if necessary, release, isolation and / or concentration of polynucleic acids from the microorganism (s) determined in the sample;
(ii) if necessary, amplification of the 16S-23S rRNA spacer region or part thereof from the microorganism (s) determined in the sample using at least one suitable primer pair;
(iii) hybridization of polynucleic acids obtained in steps (i) or (ii) with a set of probes comprising at least two probes under the same conditions of hybridization and washing, with the indicated probes selected from the sequences shown in Table 1a, or their equivalents, and / or from taxon-specific probes belonging to any of the spacer sequences shown in FIG. 1-103, while the specified taxon-specific probe is chosen such that it can hybridize in the same conditions of hybridization and washing, as at least one of the probes of Table 1a;
(iv) detecting hybrids formed in step (iii);
(v) identify the microorganism (s) present in the sample based on the differential hybridization signals obtained in step (iv).

2. The method according to claim 1, characterized in that said sample is a sample taken from the respiratory tract, and the set of probes defined in step (iii) includes at least one probe selected from the following spacer probes:
MYC-ICG-1: ACTGGATAGTGGTTGCGAGCATCTA (SEQ ID NO 1)
MYC-ICG-22: CTTCTGAATAGTGGTTGCGAGCATCT (SEQ ID NO 2)
MTB-ICG-1: GGGTGCATGACAACAAAGTTGGCCA (SEQ ID NO 3)
MTB-ICG-2: GACTTGTTCCAGGTGTTGTCCCAC (SEQ ID NO 4)
MTB-ICG-3: CGGCTAGCGGTGGCGTGTTCT (SEQ ID NO 5)
MAI-ICG-1: CAACAGCAAATGATTGCCAGACACAC (SEQ ID NO 6)
MIL-ICG-11: GAGGGGTTCCCGTCTGTAGTG (SEQ ID NO 7)
MIL-ICG-22: TGAGGGGTTCTCGTCTGTAGTG (SEQ ID NO 8)
MAC-ICG-1: CACTCGGTCGATCCGTGTGGA (SEQ ID NO 9)
MAV-ICG-1: TCGGTCCGTCCGTGTGGAGTC (SEQ ID NO 10)
MAV-ICG-22: GTGGCCGGCGTTCATCGAAA (SEQ ID NO 11)
MIN-ICG-1: GCATAGTCCTTAGGGCTGATGCGTT (SEQ ID NO 12)
MIN-ICG-2: GCTGATGCGTTCGTCGAAATGTGTA (SEQ ID NO 13)
MIN-ICG-22: CTGATGCGTTCGTCGAAATGTGT (SEQ ID NO 14)
MIN-ICG-222: TGATGCGTTCGTCGAAATGTGT (SEQ ID NO 15)
MIN-ICG-2222: GGCTGATGCGTTCGTCGAAATGTGTAA (SEQ ID NO 16)
MAL-ICG-1: ACTAGATGAACGCGTAGTCCTTGT (SEQ ID NO 17)
MHEF-ICG-1: TGGACGAAAACCGGGTGCACAA (SEQ ID NO 18)
MAH-ICG-1: GTGTAATTTCTTTTTTAACTCTTGTGTGTAAGTAAGTG (SEQ ID NO 19)
MCO-ICG-11: TGGCCGGCGTGTTCATCGAAA (SEQ ID NO 20)
MTH-ICG-11: GCACTTCAATTGGTGAAGTGCGAGCC (SEQ ID NO 21)
MTH-ICG-2: GCGTGGTCTTCATGGCCGG (SEQ ID NO 22)
MEF-ICG-11: ACGCGTGGTCCTTCGTGG (SEQ ID NO 23)
MSC-ICG-1: TCGGCTCGTTCTGAGTGGTGTC (SEQ ID NO 24)
MKA-ICG-1: GATGCGTTTGCTACGGGTAGCGT (SEQ ID NO 25)
MKA-ICG-2: GATGCGTTGCCTACGGGTAGCGT (SEQ ID NO 26)
MKA-ICG-3: ATGCGTTGCCCTACGGGTAGCGT (SEQ ID NO 27)
MKA-ICG-4: CGGGCTCTGTTCGAGAGTTGTC (SEQ ID NO 28)
MKA-iCG-5: CCCTCAGGGATTTTCTGGGTGTTG (SEQ ID NO 182)
MKA-ICG-6: GGACTCGTCCAAGAGTGTTGTCC (SEQ ID NO 183)
MKA-ICG-7: TCGGGCTTGGCCAGAGCTGTT (SEQ ID NO 184)
MKA-ICG-8: GGGTGCGCAACAGCAAGCGA (SEQ ID NO 185)
MKA-ICG-9: GATGCGTTGCCCCTACGGG (SEQ ID NO 186)
MKA-ICG-10: CCCTACGGGTAGCGTGTTCTTTTG (SEQ ID NO 187)
MCH-ICG-1: GGTGTGGACTTTGACTTCTGAATAG (SEQ ID NO 29)
MCH-ICG-2: CGGCAAAACGTCGGACTGTCA (SEQ ID NO 30)
MCH-ICG-3: GGTGTGGTCCTTGACTTATGGATAG (SEQ ID NO 210)
MGO-ICG-1: AACACCCTCGGGTGCTGTCC (SEQ ID NO 31)
MGO-ICG-2: GTATGCGTTGTCGTTCGCGGC (SEQ ID NO 32)
MGO-ICG-5: CGTGAGGGGTCATCGTCTGTAG (SEQ ID NO 33)
MUL-ICG-1: GGTTTCGGGATGTTGTCCCACC (SEQ ID NO 175)
MGV-ICG-1: CGACTGAGGTCGACGTGGTGT (SEQ ID NO 176)
MGV-ICG-2: GGTGTTTGGCATATGAATAGTGGTTGC (SEQ ID NO 177)
MGV-ICG-3: TCGGGCCGCGTGTTCGTCAAA (SEQ ID NO 211)
MXE-ICG-1: GTTGGGCAGCAGGCAGTAACC (SEQ ID NO 178)
MSI-ICG-1: CCGGCAACGGTTACGTGTTC (SEQ ID NO 179)
MFO-ICG-1: TCGTTGGATGGCCTCGCACCT (SEQ ID NO 180)
MFO-ICG-2: ACTTGGCGTGGGATGCGGGAA (SEQ ID NO 181)
MML-ICG-1: CGGATCGATTGAGTGCTTGTCCC (SEQ ID NO 188)
MML-ICG-2: TCTAAATGAACGCACTGCCGATGG (SEQ ID NO 189)
MCE-ICG-1: TGAGGGAGCCCGTGCCTGTA (SEQ ID NO 190)
MHP-ICG-1: CATGTTGGGCTTGATCGGGTGC (SEQ ID NO 191)
PA-ICG 1: TGGTGTGCTGCGTGATCCGAT (SEQ ID NO 34)
PA-ICG 2: TGAATGTTCGTGGATGAACATTGATT (SEQ ID NO 35)
PA-ICG 3: CACTGGTGATCATTCAAGTCAAG (SEQ ID NO 36)
PA-ICG 4: TGAATGTTCGT (G / A) (G / A) ATGAACATTGATTTCTGGTC (SEQ ID NO 37)
PA-ICG 5: CTCTTTCACTGGTGATCATTCAAGTCAAG (SEQ ID NO 38)
MPN-ICG 1: ATCGGTGGTAAATTAAACCCAAATCCCTGT (SEQ ID NO 49)
MPN-ICG 2: CAGTTCTGAAAGAACATTTCCGCTTCTTTC (SEQ ID NO 50)
MGE-ICG 1: CACCCATTAATTTTTTCGGTGTTAAAACCC (SEQ ID NO 51)
Mycoplasma-ICG: CAAAACTGAAAACGACAATCTTTCTAGTTCC (SEQ ID NO 52)
STAU-ICG 1: TACCAAGCAAAACCGAGTGAATAAAGAGTT (SEQ ID NO 53)
STAU-ICG 2: CAGAAGATGCGGAATAACGTGAC (SEQ ID NO 54)
STAU-ICG 3: AACGAAGCCGTATGTGAGCATTTGAC (SEQ ID NO 55)
STAU-ICG 4: GAACGTAACTTCATGTTAACGTTTGACTTAT (SEQ ID NO 56)
ACI-ICG 1: GCTTAAGTGCACAGTTCTCTAAACTGA (SEQ ID NO 57)
ACI-ICG 2: CACGGTAATTAGTGTGATCTGACGAAG (SEQ ID NO 58)
and more preferably from the following spacer probes:
MYC-ICG-1: ACTGGATAGTGGTTGCGAGCATCTA (SEQ ID NO 1)
MYC-ICG-22: CTTCTGAATAGTGGTTGCGAGCATCT (SEQ ID NO 2)
MTB-ICG-1: GGGTGCATGACAACAAAGTTGGCCA (SEQ ID NO 3)
MTB-ICG-2: GACTTGTTCCAGGTGTTGTCCCAC (SEQ ID NO 4)
MTB-ICG-3: CGGCTAGCGGTGGCGTGTTCT (SEQ ID NO 5)
MAI-ICG-1: CAACAGCAAATGATTGCCAGACACAC (SEQ ID NO 6)
MIL-ICG-11: GAGGGGTTCCCGTCTGTAGTG (SEQ ID NO 7)
MIL-ICG-22: TGAGGGGTTCTCGTCTGTAGTG (SEQ ID NO 8)
MAC-ICG-1: CACTCGGTCGATCCGTGTGGA (SEQ ID NO 9)
MAV-ICG-1: TCGGTCCGTCCGTGTGGAGTC (SEQ ID NO 10)
MAV-ICG-22: GTGGCCGGCGTTCATCGAAA (SEQ ID NO 11)
MIN-ICG-1: GCATAGTCCTTAGGGCTGATGCGTT (SEQ ID NO 12)
MAL-ICG-1: ACTAGATGAACGCGTAGTCCTTGT (SEQ ID NO 17)
MCO-ICG-11: TGGCCGGCGTGTTCATCGAAA (SEQ ID NO 20)
MTH-ICG-11: GCACTTCAATTGGTGAAGTGCGAGCC (SEQ ID NO 21)
MTH-ICG-2: GCGTGGTCTTCATGGCCGG (SEQ ID NO 22)
MEF-ICG-11: ACGCGTGGTCCTTCGTGG (SEQ ID NO 23)
MSC-ICG-1: TCGGCTCGTTCTGAGTGGTGTC (SEQ ID NO 24)
MKA-ICG-3: ATGCGTTGCCCTACGGGTAGCGT (SEQ ID NO 27)
MKA-ICG-4: CGGGCTCTGTTCGAGAGTTGTC (SEQ ID NO 28)
MKA-ICG-5: CCCTCAGGGATTTTCTGGGTGTTG (SEQ IE NO 182)
MKA-ICG-6: GGACTCGTCCAAGAGTGTTGTCC (SEQ ID NO 183)
MKA-ICG-7: TCGGGCTTGGCCAGAGCTGTT (SEQ ID NO 184)
MKA-ICG-8: GGGTGCGCAACAGCAAGCGA (SEQ ID NO 185)
MKA-ICG-9: GATGCGTTGCCCCTACGGG (SEQ ID NO 186)
MKA-ICG-10: CCCTACGGGTAGCGTGTTCTTTTG (SEQ ID NO 187)
MCH-ICG-1: GGTGTGGACTTTGACTTCTGAATAG (SEQ ID NO 29)
MCH-ICG-2: CGGCAAAACGTCGGACTGTCA (SEQ ID NO 30)
MCH-ICG-3: GGTGTGGTCCTTGACTTATGGATAG (SEQ ID NO 210)
MGO-ICG-5: CGTGAGGGGTCATCGTCTGTAG (SEQ ID NO 33)
MUL-ICG-1: GGTTTCGGGATGTTGTCCCACC (SEQ ID NO 175)
MGV-ICG-1: CGACTGAGGTCGACGTGGTGT (SEQ ID NO 176)
MGV-ICG-2: GGTGTTTGGCATATGAATAGTGGTTGC (SEQ ID NO 177)
MGV-ICG-3: TCGGGCCGCGTGTTCGTCAAA (SEQ ID NO 211)
MXE-ICG-1: GTTGGGCAGCAGGCAGTAACC (SEQ ID NO 178)
MSI-ICG-1: CCGGCAACGGTTACGTGTTC (SEQ ID NO 179)
MFO-ICG-1: TCGTTGGATGGCCTCGCACCT (SEQ ID NO 180)
MFO-ICG-2: ACTTGGCGTGGGATGCGGGAA (SEQ ID NO 181)
MML-ICG-1: CGGATCGATTGAGTGCTTGTCCC (SEQ ID NO 188)
MML-ICG-2: TCTAAATGAACGCACTGCCGATGG (SEQ ID NO 189)
MCE-ICG-1: TGAGGGAGCCCGTGCCTGTA (SEQ ID NO 190)
MHP-ICG-1: CATGTTGGGCTTGATCGGGTGC (SEQ ID NO 191)
PA-ICG 1: TGGTGTGCTGCGTGATCCGAT (SEQ ID NO 34)
PA-ICG 4: TGAATGTTCGT (G / A) (G / A) ATGAACATTGATTTCTGGTC (SEQ ID NO 37)
PA-ICG 5: CTCTTTCACTGGTGATCATTCAAGTCAAG (SEQ ID NO 38)
MPN-ICG 1: ATCGGTGGTAAATTAAACCCAAATCCCTGT (SEQ ID NO 49)
MPN-ICG 2: CAGTTCTGAAAGAACATTTCCGCTTCTTTC (SEQ ID NO 50)
MGE-ICG 1: CACCCATTAATTTTTTTTCTCTTAAAACCC (SEQ ID NO 51)
Mycoplasma-ICG: CAAAACTGAAAACGACAATCTTTCTAGTTCC (SEQ ID NO 52)
STAU-ICG 1: TACCAAGCAAAACCGAGTGAATAAAGAGTT (SEQ ID NO 53)
STAU-ICG 2: CAGAAGATGCGGAATAACGTGAC (SEQ ID NO 54)
STAU-ICG 3: AACGAAGCCGTATGTGAGCATTTGAC (SEQ ID NO 55)
STAU-ICG 4: GAACGTAACTTCATGTTAACGTTTGACTTAT (SEQ ID NO 56)
ACI-ICG 1: GCTTAAGTGCACAGTTCTCTAAACTGA (SEQ ID NO 57)
ACI-ICG 2: CACGGTAATTAGTGTGATCTGACGAAG (SEQ ID NO 58)
or equivalents of the indicated probes, and / or the indicated set of probes includes at least one taxon-specific probe belonging to the sequence of the spacer region of one of the microorganisms determined in the specified sample, and the sequence of the indicated spacer region is selected from any of the sequences presented according to SEQ ID NO 76-106, 157-174, 124, 125, 111-115, 139-144 or 126-130, and the indicated probes or equivalents may be used in combination with any probe with which you can define at least ere one of the following organisms: Haemophilus influenzae. Streptococcus pneumoniae, Moraxella catarrhalis or Bordetella pertussis.

3. The method according to claim 1, characterized in that said sample is a sample taken from the cerebrospinal fluid, and the set of probes determined in step (iii) includes at least one probe selected from the following spacer probes:
MYC-ICG-1: ACTGGATAGTGGTTGCGAGCATCTA (SEQ ID NO 1)
MYC-ICG-22: CTTCTGAATAGTGGTTGCGAGCATCT (SEQ ID NO 2)
MTB-ICG-1: GGGTGCATGACAACAAAGTTGGCCA (SEQ ID NO 3)
MTB-ICG-2: GACTTGTTCCAGGTGTTGTCCCAC (SEQ ID NO 4)
MTB-ICG-3: CGGCTAGCGGTGGCGTGTTCT (SEQ ID NO 5)
LIS-ICG 1: CAAGTAACCGAGAATCATCTGAAAGTGAATC (SEQ ID NO 39)
LMO-ICG 1: AAACAACCTTTACTTCGTAGAAGTAAATTGGTTAAG (SEQ ID NO 40)
LMO-ICG 2: TGAGAGGTTAGTACTTCTCAGTATGTTTGTTC (SEQ ID NO 41)
LMO-ICG 3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
LISP-ICG 1: CGTTTTCATAAGCGATCGCACGTT (SEQ ID NO 212)
and preferably from the following spacer probes:
MYC-ICG-1: ACTGGATAGTGGTTGCGAGCATCTA (SEQ ID NO 1)
MYC-ICG-22: CTTCTGAATAGTGGTTGCGAGCATCT (SEQ ID NO 2)
MTB-ICG-1: GGGTGCATGACAACAAAGTTGGCCA (SEQ ID NO 3)
MTB-ICG-2: GACTTGTTCCAGGTGTTGTCCCAC (SEQ ID NO 4)
MTB-ICG-3: CGGCTAGCGGTGGCGTGTTCT (SEQ ID NO 5)
LIS-ICG 1: CAAGTAACCGAGAATCATCTGAAAGTGAATC (SEQ ID NO 39)
LMO-ICG 3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
LISP-ICG 1: CGTTTTCATAAGCGATCGCACGTT (SEQ ID NO 212)
or equivalents of the indicated probes, and / or the indicated set of probes includes at least one taxon-specific probe belonging to the sequence of the spacer region of one of the microorganisms determined in the specified sample, and the sequence of the indicated spacer region is selected from any of the sequences presented according to SEQ ID NO 116, 118-121 or 213-215, and with these probes or equivalents possibly used in combination with any probe, with which you can determine at least one of the following constituent organisms: Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae.

4. The method according to claim 1, characterized in that said sample is a sample taken from the urogenital tract and the set of probes determined in step (iii) includes at least one probe selected from the following spacer probes:
CHTR-ICG 1: GGAAGAAGCCTGAGAAGGTTTCTGAC (SEQ ID NO 45)
CHTR-ICG 2: GCATTTATATGTAAGAGCAAGCATTCTATTTCA (SEQ ID NO 46)
CHTR-ICG 3: GAGTAGCGTGGTGAGGACGAGA (SEQ ID NO 47)
CHTR-ICG 4: GAGTAGCGCGGTGAGGACGAGA (SEQ ID NO 201)
CHPS-ICG 1: GGATAACTGTCTTAGGACGGTTTGAC (SEQ ID NO 48)
MGE-ICG 1: CACCCATTAATTTTTTCGGTGTTAAAACCC (SEQ ID NO 51)
Mycoplasma-ICG: CAAAACTGAAAACGACAATCTTTCTAGTTCC (SEQ ID NO 52)
or equivalents of the indicated probes, and / or the indicated set of probes includes at least one taxon-specific probe belonging to the sequence of the spacer region of one of the microorganisms determined in the specified sample, and the sequence of the indicated spacer region is selected from any of the sequences presented according to SEQ ID NO 122, 123, 197, 124 or 125, with the indicated probes or equivalents possibly being used in combination with any probe with which one can determine at least one of the following Neisseria gonorrho-eae, Haemophilus ducreyi or Streptococcus agalactiae.

5. The method according to claim 1, characterized in that said sample is a sample taken from food and the set of probes defined in step (iii) includes at least one probe selected from the following spacer probes:
LIS-ICG 1: CAAGTAACCGAGAATCATCTGAAAGTGAATC (SEQ ID NO 39)
LMO-ICG 1: AAACAACCTTTACTTCCTACAACTAAATTCCTTAAC (SEQ ID NO 40)
LMO-ICG 2: TGAGAGGTTAGTACTTCTCAGTATGTTTGTTC (SEQ ID NO 41)
LMO-ICG 3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
LIV-ICG 1: GTTAGCATAAATAGGTAACTATTTATGACACAAGTAAC (SEQ ID NO 43)
LSE-ICG 1: AGTTAGCATAAGTAGTGTAACTATTTATGACACAAG (SEQ ID NO 44)
LISP-ICG 1: CGTTTTCATAAGCGATCGCACGTT (SEQ ID NO 212)
STAU-ICG 1: TACCAAGCAAAACCGAGTGAATAAAGAGTT (SEQ ID NO 53)
STAU-ICG 2: CAGAAGATGCGGAATAACGTGAC (SEQ ID NO 54)
STAU-ICG 3: AACGAAGCCGTATGTGAGCATTTGAC (SEQ ID NO 55)
STAU-ICG 4: GAACGTAACTTCATGTTAACGTTTGACTTAT (SEQ ID NO 56)
BRU-ICG 1: CGTGCCGCCTTCGTTTCTCTTT (SEQ ID NO 59)
BRU-ICG 2: TTCGCTTCGGGGTGGATCTGTG (SEQ ID NO 60)
BRU-ICG 3: GCGTAGTAGCGTTTGCGTCGG (SEQ ID NO 193)
BRU-ICG 4: CGCAAGAAGCTTGCTCAAGCC (SEQ ID NO 194)
SALM-ICG 1: CAAAACTGACTTACGAGTCACGTTTGAG (SEQ ID NO 61)
SALM-ICG 2: GATGTATGCTTCGTTATTCCACGCC (SEQ ID NO 62)
STY-ICG 1: GGTCAAACCTCCAGGGACGCC (SEQ ID NO 63)
SED-ICG 1: GCGGTAATGTGTGAAAGCGTTGCC (SEQ ID NO 64)
YEC-ICG 1: GGAAAAGGTACTGCACGTGACTG (SEQ ID NO 198)
YEC-ICG 2: GACAGCTGAAACTTATCCCTCCG (SEQ ID NO 199)
YEC-ICG 3: GCTACCTGTTGATGTAATGAGTCAC (SEQ ID NO 200)
and preferably from the following spacer probes:
LIS-ICG 1: CAAGTAACCGAGAATCATCTGAAAGTGAATC (SEQ ID NO 39)
LMO-ICG 3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
LISP-ICG 1: CGTITTCATAAGCGATCGCACGTT (SEQ ID NO 212)
STAU-ICG 1: TACCAAGCAAAACCGAGTGAATAAAGAGTT (SEQ ID NO 53)
STAU-ICG 2: CAGAAGATGCGGAATAACGTGAC (SEQ ID NO 54)
STAU-ICG 3: AACGAAGCCGTATGTGAGCATTTGAC (SEQ ID NO 55)
STAU-ICG 4: GAACGTAACTTCATGTTAACGTTTGACTTAT (SEQ ID NO 56)
BRU-ICG 2: TTCGCTTCGGGGTGGATCTGTG (SEQ ID NO 60)
BRU-ICG 3: GCGTAGTAGCGTTTGCGTCGG (SEQ ID NO 193)
BRU-ICG 4: CGCAAGAAGCTTGCTCAAGCC (SEQ ID NO 194)
SALM-ICG 1: CAAAACTGACTTACGAGTCACGTTTGAG (SEQ ID NO 61)
YEC-ICG 1: GGAAAAGGTACTGCACGTGACTG (SEQ ID NO 198)
YEC-ICG 2: GACAGCTGAAACTTATCCCTCCG (SEQ ID NO 199)
YEC-ICG 3: GCTACCTGTTGATGTAATGAGTCAC (SEQ ID NO 200)
or equivalents of the indicated probes, and / or the indicated set of probes includes at least one taxon-specific probe belonging to the sequence of the spacer region of one of the microorganisms determined in the specified sample, and the sequence of the indicated spacer region is selected from any of the sequences presented according to SEQ ID NO 116, 118-121, 213-215, 139-144, 131, 132, 154, 133-138, 195 or 196, with the indicated probes or equivalents possibly being used in combination with any probe with which you can determine the strains Campylobacter species.

6. The method according to claim 1, characterized in that said sample is a sample taken from the patient’s gastrointestinal tract and the set of probes determined in step (iii) includes at least one probe selected from the following spacer probes:
SALM-ICG 1: CAAAACTGACTTACGAGTCACGTTTGAG (SEQ ID NO 61)
SALM-ICG 2: GATGTATGCTTCGTTATTCCACGCC (SEQ ID NO 62)
STY-ICG 1: GGTCAAACCTCCAGGGACGCC (SEQ ID NO 63)
SED-ICG 1: GCGGTAATGTGTGAAAGCGTTGCC (SEQ ID NO 64)
YEC-ICG 1: GGAAAAGGTACTGCACGTGACTG (SEQ ID NO 198)
YEC-ICG 2: GACAGCTGAAACTTATCCCTCCG (SEQ ID NO 199)
YEC-ICG 3: GCTACCTGTTGATGTAATGAGTCAC (SEQ ID NO 200)
and preferably from the following spacer probes:
SALM-ICG 1: CAAAACTGACTTACGAGTCACGTTTGAG (SEQ ID NO 61)
YEC-ICG 1: GGAAAAGGTACTGCACGTGACTG (SEQ ID NO 198)
YEC-ICG 2: GACAGCTGAAACTTATCCCTCCG (SEQ ID NO 199)
YEC-ICG 3: GCTACCTGTTGATGTAATGAGTCAC (SEQ ID NO 200)
or equivalents of the indicated probes, and / or the indicated set of probes includes at least one taxon-specific probe belonging to the sequence of the spacer region of one of the microorganisms determined in the specified sample, and the sequence of the indicated spacer region is selected from any of the sequences presented according to SEQ ID NO 133-138 or 195-196, with the indicated probes or equivalents possibly being used in combination with any probe with which it is possible to determine Campylobacter species.

7. The method according to claim 1 for determining and identifying in the sample one or more strains of the species and subspecies of Mycobacterium, in which stage (iii) involves hybridization with at least one of the following probes:
MYC-ICG-1: ACTGGATAGTGGTTGCGAGCATCTA (SEQ ID NO 1)
MYC-ICG-22: CTTCTGAATAGTGGTTGCGAGCATCT (SEQ ID NO 2)
MTB-ICG-1: GGGTGCATGACAACAAAGTTGGCCA (SEQ ID NO 3)
MTB-ICG-2: GACTTGTTCCAGGTG1TGTCCCAC (SEQ ID NO 4)
MTB-ICG-3: CGGCTAGCGGTGGCGTGTTCT (SEQ ID NO 5)
MAI-ICG-1: CAACAGCAAATGATTGCCAGACACAC (SEQ ID NO 6)
MIL-ICG-11: GAGGGGTTCCCGTCTGTAGTG (SEQ ID NO 7)
MIL-ICG-22: TGAGGGGTTCTCGTCTGTAGTG (SEQ ID NO 8)
MAC-ICG-1: CACTCGGTCGATCCGTGTGGA (SEQ ID NO 9)
MAV-ICG-1: TCGGTCCGTCCGTGTGGAGTC (SEQ ID NO 10)
MAV-ICG-22: GTGGCCGGCGTTCATCGAAA (SEQ ID NO 11)
MIN-ICG-1: GCATAGTCCTTAGGGCTGATGCGTT (SEQ ID NO 12)
MIN-ICG-2: GCTGATGCGTTCGTCGAAATGTGTA (SEQ ID NO 13)
MIN-ICG-22: CTGATGCGTTCGTCGAAATGTGT (SEQ ID NO 14)
MIN-ICG-222: TGATGCGTTCGTCGAAATGTGT (SEQ ID NO 15)
MIN-ICG-2222: GGCTGATGCGTTCGTCGAAATGTGTAA (SEQ ID NO 16)
MAL-ICG-1: ACTAGATGAACGCGTAGTCCTTGT (SEQ ID NO 17)
MHEF-ICG-1: TGGACGAAAACCGGGTGCACAA (SEQ ID NO 18)
MAH-ICG-1: GTGTAATTTCTTTTTTAACTCTTGTGTGTAAGTAAGTG (SEQ ID NO 19)
MCO-ICG-11: TGGCCGGCGTGTTCATCGAAA (SEQ ID NO 20)
MTH-ICG-11: GCACTTCAATTGGTGAAGTGCGAGCC (SEQ ID NO 21)
MTH-ICG-2: GCGTGGTCTTCATGGCCGG (SEQ ID NO 22)
MEF-ICG-11: ACGCGTGGTCCTTCGTGG (SEQ ID NO 23)
MSC-ICG-1: TCGGCTCGTTCTGAGTGGTGTC (SEQ ID NO 24)
MKA-ICG-1: GATGCGTTTGCTACGGGTAGCGT (SEQ ID NO 25)
MKA-ICG-2: GATGCGTTGCCTACGGGTAGCGT (SEQ ID NO 26)
MKA-ICG-3: ATGCGTTGCCCTACGGGTAGCGT (SEQ ID NO 27)
MKA-ICG-4: CGGGCTCTGTTCGAGAGTTGTC (SEQ ID NO 28)
MKA-ICG-5: CCCTCAGGGATTTTCTGGGTGTTG (SEQ ID NO 182)
MKA-ICG-6: GGACTCGTCCAAGAGTGTTGTCC (SEQ ID NO 183)
MKA-ICG-7: TCGGGCTTGGCCAGAGCTGTT (SEQ ID NO 184)
MKA-ICG-8: GGGTGCGCAACAGCAAGCGA (SEQ ID NO 185)
MKA-ICG-9: GATGCGTTGCCCCTACGGG (SEQ ID NO 186)
MKA-ICG-10: CCCTACCCCTACCCTCTTCTTTTG (SEQ ID NO 187)
MCH-ICG-1: GGTGTGGACTTTGACTTCTGAATAG (SEQ ID NO 29)
MCH-ICG-2: CGGCAAAACGTCGGACTGTCA (SEQ ID NO 30)
MGO-ICG-1: AACACCCTCGGGTGCTGTCC (SEQ ID NO 31)
MGO-ICG-2: GTATGCGTTGTCGTTCGCGGC (SEQ ID NO 32)
MGO-ICG-5: CGTGAGGGGTCATCGTCTGTAG (SEQ ID NO 33)
MUL-ICG-1: GGTTTCGGGATGTTGTCCCACC (SEQ ID NO 175)
MGV-ICG-1: CGACTGAGGTCGACGTGGTGT (SEQ ID NO 176)
MGV-ICG-2: GGTGTTTGGCATATGAATAGTGGTTGC (SEQ ID NO 177)
MXE-ICG-1: GTTGGGCAGCAGGCAGTAACC (SEQ ID NO 178)
MSI-ICG-1: CCGGCAACGGTTACGTGTTC (SEQ ID NO 179)
MFO-ICG-1: TCGTTGGATGGCCTCGCACCT (SEQ ID NO 180)
MFO-ICG-2: ACTTGGCGTGGGATGCGGGAA (SEQ ID NO 181)
MML-ICG-1: CGGATCGATTGAGTGCTTGTCCC (SEQ ID NO 188)
MML-ICG-2: TCTAAATGAACGCACTGCCGATGG (SEQ ID NO 189)
MCE-ICG-1: TGAGGGAGCCCGTGCCTGTA (SEQ ID NO 190)
MHP-ICG-1: CATGTTGGGC1TGATCGGGTGC (SEQ ID NO 191)
and more preferably with at least one probe from the following narrower group of spacer probes:
MYC-ICG-1: ACTGGATAGTGGTTGCGAGCATCTA (SEQ ID NO 1)
MYC-ICG-22: CTTCTGAATAGTGGTTGCGAGCATCT (SEQ ID NO 2)
MTB-ICG-1: GGGTGCATGACAACAAAGTTGGCCA (SEQ ID NO 3)
MTB-ICG-2: GACTTGTTCCAGGTGTTGTCCCAC (SEQ ID NO 4)
MTB-ICG-3: CGGCTAGCGGTGGCGTGTTCT (SEQ ID NO 5)
MAI-ICG-1: CAACAGCAAATGATTGCCAGACACAC (SEQ ID NO 6)
MIL-ICG-11: GAGGGGTTCCCGTCTGTAGTG (SEQ ID NO 7)
MIL-ICG-22: TGAGGGGTTCTCGTCTGTAGTG (SEQ ID NO 8)
MAC-ICG-1: CACTCGGTCGATCCGTGTGGA (SEQ ID NO 9)
MAV-ICG-1: TCGGTCCGTCCGTGTGGAGTC (SEQ ID NO 10)
MAV-ICG-22: GTGGCCGGCGTTCATCGAAA (SEQ ID NO 11)
MIN-ICG-1: GCATAGTCCTTAGGGCTGATGCGTT (SEQ ID NO 12)
MAL-ICG-1: ACTAGATGAACGCGTAGTCCTTGT (SEQ ID NO 17)
MCO-ICG-11: TGGCCGGCGTGTTCATCGAAA (SEQ ID NO 20)
MTH-ICG-11: GCACTTCAATTGGTGAAGTGCGAGCC (SEQ ID NO 21)
MTH-ICG-2: GCGTGGTCTTCATGGCCGG (SEQ ID NO 22)
MEF-ICG-11: ACGCGTGGTCCTTCGTGG (SEQ ID NO 23)
MSC-ICG-1: TCGGCTCGTTCTGAGTGGTGTC (SEQ ID NO 24)
MKA-ICG-3: ATGCGTTGCCCTACGGGTAGCGT (SEQ ID NO 27)
MKA-ICG-4: CGGGCTCTGTTCGAGAGTTGTC (SEQ ID NO 28)
MKA-ICG-5: CCCTCAGGGATTTTCTGGGTGTTG (SEQ ID NO 182)
MKA-ICG-6: GGACTCGTCCAAGAGTGTTGTCC (SEQ ID NO 183)
MKA-ICG-7: TCGGGCTTGGCCAGAGCTGTT (SEQ ID NO 184)
MKA-ICG-8: GGGTGCGCAACAGCAAGCGA (SEQ ID NO 185)
MKA-ICG-9: GATGCGTTGCCCCTACGGG (SEQ ID NO 186)
MKA-ICG-10: CCCTACGGGTAGCGTGTTCTTTTG (SEQ ID NO 187)
MCH-ICG-1: GGTGTGGACTTTGACTTCTGAATAG (SEQ ID NO 29)
MCH-ICG-2: CGGCAAAACGTCGGACTGTCA (SEQ ID NO 30)
MCH-ICG-3: GGTGTGGTCCTTGACTTATGGATAG (SEQ ID NO 210)
MGO-ICG-5: CGTGAGGGGTCATCGTCTGTAG (SEQ ID NO 33)
MUL-ICG-1: GGTTTCGGGATGTTGTCCCACC (SEQ ID NO 175)
MGV-ICG-1: CGACTGAGGTCGACGTGGTGT (SEQ ID NO 176)
MGV-ICG-2: GGTGTTTGGCATATGAATAGTGGTTGC (SEQ ID NO 177)
MGV-ICG-3: TCGGGCCGCGTGTTCGTCAAA (SEQ ID NO 211)
MXE-ICG-1: GTTGGGCAGCAGGCAGTAACC (SEQ ID NO 178)
MSI-ICG-1: CCGGCAACGGTTACGTGTTC (SEQ ID NO 179)
MFO-ICG-1: TCGTTGGATGGCCTCGCACCT (SEQ ID NO 180)
MFO-ICG-2: ACTTGGCGTGGGATGCGGGAA (SEQ ID NO 181)
MML-ICG-1: CGGATCGATTGAGTGCTTGTCCC (SEQ ID NO 188)
MML-ICG-2: TCTAAATGAACGCACTGCCGATGG (SEQ ID NO 189)
MCE-ICG-1: TGAGGGAGCCCGTGCCTGTA (SEQ ID NO 190)
MHP-ICG-1: CATGTTGGGCTTGATCGGGTGC (SEQ ID NO 191)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 76-110 or 157-174, providing for specific hybridization of the indicated probe with the Mycobacterium species.

8. The method according to claim 7 for determining and identifying in the sample one or more Mycobacterium tuberculosis complex strains, in which step (iii) involves hybridization with at least one of the following probes:
MTB-ICG-1: GGGTGCATGACAACAAAGTTGGCCA (SEQ ID NO 3)
MTB-ICG-2: GACTTGTTCCAGGTGTTGTCCCAC (SEQ ID NO 4)
MTB-ICG-3: CGGCTAGCGGTGGCGTGTTCT (SEQ ID NO 5)
or with equivalents of the above probes, and / or with any probe of SEQ ID NO 76, providing for specific hybridization of said probe with the M. tuberculosis complex.

9. The method according to claim 7 for detecting and identifying one or more strains of Mycobacterium from the MAIS complex, wherein step (iii) involves hybridization with at least one of the following probes:
MAI-ICG-1: CAACAGCAAATGATTGCCAGACACAC (SEQ ID NO 6)
MIL-ICG-11: GAGGGGTTCCCGTCTGTAGTG (SEQ ID NO 7)
MIL-ICG-22: TGAGGGGTTCTCGTCTGTAGTG (SEQ ID NO 8)
MAC-ICG-1: CACTCGGTCGATCCGTGTGGA (SEQ ID NO 9)
MAV-ICG-1: TCGGTCCGTCCGTGTGGAGTC (SEQ ID NO 10)
MAV-ICG-22: GTGGCCGGCGTTCATCGAAA (SEQ ID NO 11)
MIN-ICG-1: GCATAGTCCTTAGGGCTGATGCGTT (SEQ ID NO 12)
MIN-ICG-2: GCTGATGCGTTCGTCGAAATGTGTA (SEQ ID NO 13)
MIN-ICG-22: CTGATGCGTTCGTCGAAATGTGT (SEQ ID NO 14)
MIN-ICG-222: TGATGCGTTCGTCGAAATGTGT (SEQ ID NO 15)
MIN-ICG-2222: GGCTGATGCGTTCGTCGAAATGTGTAA (SEQ ID NO 16)
MAL-ICG-1: ACTAGATGAACGCGTAGTCCTTGT (SEQ ID NO 17)
MHEF-ICG-1: TGGACGAAAACCGGGTGCACAA (SEQ ID NO 18)
MAH-ICG-1: GTGTAATTTCTTTTTTAACTCTTGTGTGTAAGTAAGTG (SEQ ID NO 19)
MCO-ICG-11: TGGCCGGCGTGTTCATCGAAA (SEQ ID NO 20)
MTH-ICG-11: GCACTTCAATTGGTGAAGTGCGAGCC (SEQ ID NO 21)
MTH-ICG-2: GCGTGGTCTTCATGGCCGG (SEQ ID NO 22)
MEF-ICG-11: ACGCGTGGTCCTTCGTGG (SEQ ID NO 23)
MSC-ICG-1: TCGGCTCGTTCTGAGTGGTGTC (SEQ ID NO 24)
or with equivalents of the indicated probes, and / or with any probe from SEQ ID NO 77-100 or 108-110, providing specific hybridization of said probe with strains of the MAIS-complex.

10. The method according to claim 9 for determining and identifying in the sample one or more strains of M. avium and M. paratuberculosis, in which stage (iii) involves hybridization with at least one of the following probes:
MAV-ICG-1: TCGGTCCGTCCGTGTGGAGTC (SEQ ID NO 10)
MAV-ICG-22: GTGGCCGGCGTTCATCGAAA (SEQ ID NO 11)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 77 and 78, providing specific hybridization of said probe with M. avium and M. paratuberculosis.

11. The method of claim 9 for detecting and identifying one or more Mycobacterium intracellulare and MIC strains in a sample, in which step (iii) involves hybridization with at least one of the following probes:
MAI-ICG-1: CAACAGCAAATGATTGCCAGACACAC (SEQ ID NO 6)
MIL-ICG-11: GAGGGGTTCCCGTCTGTAGTG (SEQ ID NO 7)
MIL-ICG-22: TGAGGGGTTCTCGTCTGTAGTG (SEQ ID NO 8)
MAC-ICG-1: CACTCGGTCGATCCGTGTGGA (SEQ ID NO 9)
MIN-ICG-1: GCATAGTCCTTAGGGCTGATGCGTT (SEQ ID NO 12)
MIN-ICG-2: GCTGATGCGTTCGTCGAAATGTGTA (SEQ ID NO 13)
MIN-ICG-22: CTGATGCGTTCGTCGAAATGTGT (SEQ ID NO 14)
MIN-ICG-222: TGATGCGTTCGTCGAAATGTGT (SEQ ID NO 15)
MIN-ICG-2222: GGCTGATGCGTTCGTCGAAATGTGTAA (SEQ ID NO 16)
MAL-ICG-1: ACTAGATGAACGCGTAGTCCTTGT (SEQ ID NO 17)
MHEF-ICG-1: TGGACGAAAACCGGGTGCACAA (SEQ ID NO 18)
MAH-ICG-1: GTGTAATTTCTTTTTTAACTCTTGTGTGTAAGTAAGTG (SEQ ID NO 19)
MCO-ICG-11: TGGCCGGCGTGTTCATCGAAA (SEQ ID NO 20)
MTH-ICG-11: GCACTTCAATTGGTGAAGTGCGAGCC (SEQ ID NO 21)
MTH-ICG-2: GCGTGGTCTTCATGGCCGG (SEQ ID NO 22)
MEF-ICG-11: ACGCGTGGTCCTTCGTGG (SEQ ID NO 23)
or with equivalents of these probes, and / or with any probe from SEQ ID NO 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98 or 99, providing specific hybridization of the specified probe with strains of M. intracellulare and MTC-strains.

12. The method according to claim 9 for determining and identifying in the sample one or more strains of Mycobacterium intracellulare, in which stage (iii) includes hybridization with the following probe:
MIN-ICG-1: GCATAGTCCTTAGGGCTGATGCGTT (SEQ ID NO 12)
or with equivalents of the specified probe, and / or with any probe of SEQ ID NO 89, providing specific hybridization of the specified probe with M. intracellulare.

13. The method according to claim 9 for determining and identifying in the sample one or more strains of Mycobacterium scrofulaceum, in which stage (iii) includes hybridization with the following probe:
MSC-ICG-1: TCGGCTCGTTCTGAGTGGTGTC (SEQ ID NO 24)
or with equivalents of the specified probe, and / or with any probe of SEQ ID NO 100, providing specific hybridization of the specified probe with strains of M. scrofulaceum.

14. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium kansasii, in which stage (iii) involves hybridization with at least one of the following probes:
MKA-ICG-1: GATGCGTTTGCTACGGGTAGCGT (SEQ ID NO 25)
MKA-ICG-2: GATGCGTTGCCTACGGGTAGCGT (SEQ ID NO 26)
MKA-ICG-3: ATGCGTTGCCCTACGGGTAGCGT (SEQ ID NO 27)
MKA-ICG-4: CGGGCTCTGTTCGAGAGTTGTC (SEQ ID NO 28)
MKA-ICG-5: CCCTCAGGGATTTTCTGGGTGTTG (SEQ ID NO 182)
MKA-ICG-6: GGACTCGTCCAAGAGTGTTGTCC (SEQ ID NO 183)
MKA-ICG-7: TCGGGCTTGGCCAGAGCTGTT (SEQ ID NO 184)
MKA-ICG-8: GGGTGCGCAACAGCAAGCGA (SEQ ID NO 185)
MKA-ICG-9: GATGCGTTGCCCCTACGGG (SEQ ID NO 186)
MKA-ICG-10: CCCTACGGGTAGCGTGTTCTTTTG (SEQ ID NO 187)
and more preferably with:
MKA-ICG-3: ATGCGTTGCCCTACGGGTAGCGT (SEQ ID NO 27)
MKA-ICG-4: CGGGCTCTGTTCGAGAGTTGTC (SEQ ID NO 28)
MKA-ICG-5: CCCTCAGGGATTTTCTGGGTGTTG (SEQ ID NO 182)
MKA-ICG-6: GGACTCGTCCAAGAGTGTTGTCC (SEQ ID NO 183)
MKA-ICG-7: TCGGGCTTGGCCAGAGCTGTT (SEQ ID NO 184)
MKA-ICG-8: GGGTGCGCAACAGCAAGCGA (SEQ ID NO 185)
MKA-ICG-9: GATGCGTTGCCCCTACGGG (SEQ ID NO 186)
MKA-ICG-10: CCCTACGGGTAGCGTGTTCTTTTG (SEQ ID NO 187)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 101, 167, 168 or 169, providing specific hybridization of said probe with M. kansasii strains.

15. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium chelonae, in which stage (iii) involves hybridization with at least one of the following probes:
MCH-ICG-1: GGTGTGGACTTTGACTTCTGAATAG (SEQ ID NO 29)
MCH-ICG-2: CGGCAAAACGTCGGACTGTCA (SEQ ID NO 30)
MCH-ICG-3: GGTGTGGTCCTTGACTTATGGATAG (SEQ ID NO 210)
or with equivalents of the above probes, and / or with any probe from SEQ ID NO 102, 103 or 174, providing specific hybridization of said probe with M. chelonae.

16. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium gordonae, in which stage (iii) involves hybridization with at least one of the following probes:
MGO-ICG-1: AACACCCTCGGGTGCTGTCC (SEQ ID NO 31)
MGO-ICG-2: GTATGCGTTGTCGTTCGCGGC (SEQ ID NO 32)
MGO-ICG-5: CGTGAGGGGTCATCGTCTGTAG (SEQ ID NO 33)
and most preferably with:
MGO-ICG-5: CGTGAGGGGTCATCGTCTGTAG (SEQ ID NO 33)
or with equivalents of the indicated probes, and / or with any probe from SEQ ID NO 104, 105 or 106, providing specific hybridization of said probe with M. gordonae.

17. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium ulcerans or M. marinum, in which stage (iii) involves hybridization with the following probe:
MUL-ICG-1: GGTTTCGGGATGTTGTCCCACC (SEQ ID NO 175)
or with equivalents of the specified probe, and / or with any probe of SEQ ID NO 157, providing specific hybridization of the specified probe with M. ulcerans and M. marinum.

18. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium genavense, in which stage (iii) involves hybridization with at least one of the following probes:
MGV-ICG-1: CGACTGAGGTCGACGTGGTGT (SEQ ID NO 176)
MGV-ICG-2: GGTGTTTGGCATATGAATAGTGGTTGC (SEQ ID NO 177)
MGV-ICG-3: TCGGGCCGCGTGTTCGTCAAA (SEQ ID NO 211)
or with equivalents of the above probes, and / or with any probe from SEQ ID NO 158, 159, 160, 161 or 162, providing for specific hybridization of said probe with M. genavense.

19. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium xenopi, in which stage (iii) includes hybridization with the following probe:
MXE-ICG-1: GTTGGGCAGCAGGCAGTAACC (SEQ ID NO 178)
or with equivalents of the indicated probe, and / or with any probe of SEQ ID NO 163, which provides for specific hybridization of said probe with M. xenopi.

20. The method according to claim 7 for the determination and identification in the sample of one or more strains of Mycobacterium simiae, in which stage (iii) includes hybridization with the following probe:
MSI-ICG-1: CCGGCAACGGTTACGTGTTC (SEQ ID NO 179)
or with equivalents of said probe, and / or with any probe of SEQ ID NO 164 or 165, providing for specific hybridization of said probe with M. simiae.

21. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium fortuitum, in which stage (iii) involves hybridization with at least one of the following probes:
MFO-ICG-1: TCGTTGGATGGCCTCGCACCT (SEQ ID NO 180)
MFO-ICG-2: ACTTGGCGTGGGATGCGGGAA (SEQ ID NO 181)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 166, providing specific hybridization of said probe with M. fortuitum.

22. The method according to claim 7 for determining and identifying in the sample one or more strains of Mycobacterium celatum, in which stage (iii) includes hybridization with the following probe:
MCE-ICG-1: TGAGGGAGCCCGTGCCTGTA (SEQ ID NO 190)
or with equivalents of the specified probe, and / or with any probe of SEQ ID NO 170, providing specific hybridization of the specified probe with M. celatum.

23. The method according to claim 7 for determining and identifying one or more strains of Mycobacterium haemophilum in a sample, characterized in that step (iii) involves hybridization with the following probe:
MHP-ICG-1: CATGTTGGGCTTGATCGGGTGC (SEQ ID NO 191)
or with equivalents of the specified probe, and / or with any probe of SEQ ID NO 171, 172 or 173, providing specific hybridization of said probe with M. haemophilum.

24. The method of claim 7 for detecting and identifying one or more Mycobacterium strains in a sample, wherein step (iii) involves hybridization with at least one of the following probes:
MYC-ICG-1: ACTGGATAGTGGTTGCGAGCATCTA (SEQ ID NO 1)
MYC-ICG-22: CTTCTGAATAGTGGTTGCGAGCATCT (SEQ ID NO 2)
or with equivalents of the indicated probes.

25. The method according to claim 1 for detecting and identifying one or more Mycoplasma strains in a sample, wherein step (iii) involves hybridization with at least one of the following probes:
MPN-ICG-1: ATCGGTGGTAAATTAAACCCAAATCCCTGT (SEQ ID NO 49)
MPN-ICG-2: CAGTTCTGAAAGAACATTTCCGCTTCTTTC (SEQ ID NO 50)
MGE-ICG-1: CACCCATTAATTTTTTCGGTGTTAAAACCC (SEQ ID NO 51)
Mycoplasma-ICG: CAAAACTGAAAACGACAATCTTTCTAGTTCC (SEQ ID NO 52)
or with equivalents of the indicated probes, and / or with any probe from SEQ ID NO 124 or 125, providing for specific hybridization of the indicated probe with Mycoplasma species.

26. The method according to p. 25 for determining and identifying in the sample one or more strains of Mycoplasma pneumoniae, in which stage (iii) includes hybridization with at least one of the following probes:
MPN-ICG-1: ATCGGTGGTAAATTAAACCCAAATCCCTGT (SEQ ID NO 49)
MPN-ICG-2: CAGTTCTGAAAGAACATTTCCGCTTCTTTC (SEQ ID NO 50)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 125, providing for specific hybridization of the indicated probe with Mycoplasma pneumoniae species.

27. The method according to p. 25 for the determination and identification in the sample of one or more strains of Mycoplasma genitalium, in which stage (iii) includes hybridization with the following probe:
MGE-ICG-1: CACCCATTAATTTTTTCGGTGTTAAAACCC (SEQ ID NO 51)
or with equivalents of the indicated probe, and / or with any probe of SEQ ID NO 124, which provides for specific hybridization of the indicated probe with the Mycoplasma genitalium species.

28. The method according to claim 1 for determining and identifying in the sample one or more strains of Pseudomonas, in which stage (iii) involves hybridization with at least one of the following probes:
PA-ICG-1: TGGTGTGCTGCGTGATCCGAT (SEQ ID NO 34)
PA-ICG-2: TGAATGTTCGTGGATGAACATTGATT (SEQ ID NO 35)
PA-ICG-3: CACTGGTGATCATTCAAGTCAAG (SEQ ID NO 36)
PA-ICG-4: TGAATGTTCGT (G / A) (G / A) ATGAACATTGATTTCTGGTC (SEQ ID NO 37)
PA-ICG-5: CTCTTTCACTGGTGATCATTCAAGTCAAG (SEQ ID NO 38)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 111, 112, 113, 114 or 115, providing specific hybridization of said probe with strains of Pseudomonas.

29. The method according to p. 28 for the determination and identification in the sample of one or more strains of Pseudomonas aeruginosa, in which stage (iii) includes hybridization with at least one of the following probes:
PA-ICG-1: TGGTGTGCTGCGTGATCCGAT (SEQ ID NO 34)
PA-ICG-2: TGAATGTTCGTGGATGAACATTGATT (SEQ ID NO 35)
PA-ICG-3: CACTGGTGATCATTCAAGTCAAG (SEQ ID NO 36)
PA-ICG-4: TGAATGTTCGT (G / A) (G / A) ATGAACATTGATTTCTGGTC (SEQ ID NO 37)
PA-ICG-5: CTCTTTCACTGGTGATCATTCAAGTCAAG (SEQ ID NO 38)
and most preferably with at least one of the following probes:
PA-ICG-1: TGGTGTGCTGCGTGATCCGAT (SEQ ID NO 34)
PA-ICG-4: TGAATGTTCGT (G / A) (G / A) ATGAACATTGATTTCTGGTC (SEQ ID NO 37)
PA-ICG-5: CTCTTTCACTGGTGATCATTCAAGTCAAG (SEQ ID NO 38)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 111, providing specific hybridization of said probe with Pseudomonas aeruginosa.

30. The method according to claim 1 for determining and identifying in the sample one or more Staphylococcus species, wherein step (iii) involves hybridization with at least one of the following probes:
STAU-ICG-1: TACCAAGCAAAACCGAGTGAATAAAGAGTT (SEQ ID NO 53)
STAU-ICG-2: CAGAAGATGCGGAATAACGTGAC (SEQ ID NO 54)
STAU-ICG-3: AACGAAGCCGTATGTGAGCATTTGAC (SEQ ID NO 55)
STAU-ICG-4: GAACGTAACTTCATGTTAACGTTTGACTTAT (SEQ ID NO 56)
or with equivalents of the indicated probes, and / or with any probe from SEQ ID NO 139, 140, 141, 142, 143 or 144, providing specific hybridization of the indicated probe with the Staphylococcus species.

31. The method according to claim 30 for detecting and identifying one or more strains of Staphylococcus aureus in a sample, wherein step (iii) involves hybridization with at least one, and preferably both of the following probes:
STAU-ICG-3: AACGAAGCCGTATGTGAGCATTTGAC (SEQ ID NO 55)
STAU-ICG-4: GAACGTAACTTCATGTTAACGTTTGACTTAT (SEQ ID NO 56)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 139, 140, 141, 142 or 143, providing specific hybridization of said probe with Staphylococcus aureus.

32. The method according to claim 30 for detecting and identifying one or more strains of Staphylococcus epidermidis in a sample, characterized in that step (iii) involves hybridization with any probe of SEQ ID NO 44 providing specific hybridization with Staphylococcus epidermidis.

33. The method according to claim 1 for determining and identifying in the sample one or more Acinetobacter strains, wherein step (iii) involves hybridization with at least one of the following probes:
ACI-ICG-1: GCTTAAGTGCACAGTGCTCTAAACTGA (SEQ ID NO 57)
ACI-ICG-2: CACGGTAATTAGTGTGATCTGACGAAG (SEQ ID NO 58)
or with equivalents of the indicated probes, and / or with any probe from SEQ ID NO 126, 127, 128, 129 or 130, providing specific hybridization of the indicated probe with the Acinetobacter species.

34. The method according to p for the determination and identification in the sample of one or more strains of Acinetobacter baumanii, in which stage (iii) includes hybridization with at least one of the following probes:
ACI-ICG-1: GCTTAAGTGCACAGTGCTCTAAACTGA (SEQ ID NO 57)
ACI-ICG-2: CACGGTAATTAGTGTGATCTGACGAAG (SEQ ID NO 58)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 126, providing specific hybridization of said probe with Acinetobacter baumanii.

35. The method according to claim 1 for determining and identifying in the sample one or more Listeria strains, wherein stage (iii) involves hybridization with at least one of the following probes:
LIS-ICG-1: CAAGTAACCGAGAATCATCTGAAAGTGAATC (SEQ ID NO 39)
LMO-ICG-1: AAACAACCTTTACTTCGTAGAAGTAAATTGGTTAAG (SEQ ID NO 40)
LMO-ICG-2: TGAGAGGTTAGTACTTCTCAGTATGTTTGTTC (SEQ ID NO 41)
LMO-ICG-3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
LIV-ICG-1: GTTAGCATAAATAGGTAACTATTTATGACACAAGTAAC (SEQ ID NO 43)
LSE-ICG-1: AGTTAGCATAAGTAGTGTAACTATTTATGACACAAG (SEQ ID NO)
LISP-ICG-1: CGTTTTCATAAGCGATCGCACGTT (SEQ ID NO 212)
and most preferably with at least one of the following probes:
LIS-ICG-1: CAAGTAACCGAGAATCATCTGAAAGTGAATC (SEQ ID NO 39)
LMO-ICG-3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
LISP-ICG-1: CGTTTTCATAAGCGATCGCACGTT (SEQ ID NO 212)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 116, 118, 119, 120, 121, 213, 214 or 215, providing for specific hybridization of the indicated probe with the Listeria species.

36. The method according to p. 35 for the determination and identification in the sample of one or more strains of Listeria monocytogenes, in which stage (iii) involves hybridization with at least one of the following probes:
LMO-ICG-1: AAACAACCTTTACTTCGTAGAAGTAAATTGGTTAAG (SEQ ID NO 40)
LMO-ICG-2: TGAGAGGTTAGTACTTCTCAGTATGTTTGTTC (SEQ ID NO 41)
LMO-ICG-3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
and most preferably with the following probe:
LMO-ICG-3: AGGCACTATGCTTGAAGCATCGC (SEQ ID NO 42)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 118 or 120, providing specific hybridization of said probe with Listeria monocytogenes.

37. The method according to claim 1 for determining and identifying in the sample one or more Brucella strains, in which step (iii) involves hybridization with at least one of the following probes:
BRU-ICG-1: CGTGCCGCCTTCGTTTCTCTTT (SEQ ID NO 59)
BRU-ICG-2: TTCGCTTCGGGGTGGATCTGTG (SEQ ID NO 60)
BRU-ICG-3: GCGTAGTAGCGTTTGCGTCGG (SEQ ID NO 193)
BRU-ICG-4: CGCAAGAAGCTTGCTCAAGCC (SEQ ID NO 194)
and most preferably with the following probe:
BRU-ICG-2: TTCGCTTCGGGGTGGATCTGTG (SEQ ID NO 60)
BRU-ICG-3: GCGTAGTAGCGTTTGCGTCGG (SEQ ID NO 193)
BRU-ICG-4: CGCAAGAAGCTTGCTCAAGCC (SEQ ID NO 194)
or with equivalents of the indicated probes, and / or with any probe from SEQ ID NO 131, 132 or 154, providing specific hybridization of said probe with Brucella strains.

38. The method according to claim 1 for the determination and identification in the sample of one or more strains of Salmonella, in which stage (iii) includes hybridization with at least one of the following probes:
SALM-ICG-1: CAAAACTGACTTACGAGTCACGTTTGAG (SEQ ID NO 61)
SALM-ICG-2: GATGTATGCTTCGTTATTCCACGCC (SEQ ID NO 62)
STY-ICG-1: GGTCAAACCTCCAGGGACGCC (SEQ ID NO 63)
SED-ICG-1: GCGGTAATGTGTGAAAGCGTTGCC (SEQ ID NO 64)
and most preferably with the following probe:
SALM-ICG-1: CAAAACTGACTTACGAGTCACGTTTGAG (SEQ ID NO 61)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 133, 134, 135, 136, 137 or 138, providing specific hybridization of the indicated probe with Salmonella strains.

39. The method according to claim 1 for determining and identifying in the sample one or more strains of Chlamydia, in which stage (iii) includes hybridization with at least one of the following probes:
CHTR-ICG-1: GGAAGAAGCCTGAGAAGGTTTCTGAC (SEQ ID NO 45)
CHTR-ICG-2: GCATTTATATGTAAGAGCAAGCATTCTATTTCA (SEQ ID NO 46)
CHTR-ICG-3: GAGTAGCGTGGTGAGGACGAGA (SEQ ID NO 47)
CHTR-ICG-4: GAGTAGCGCGGTGAGGACGAGA (SEQ ID NO 201)
CHPS-ICG-1: GGATAACTGTCTTAGGACGGTTTGAC (SEQ ID NO 48)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 122, 123 or 197, providing specific hybridization of said probe with Chlamydia strains.

40. The method of claim 39 for detecting and identifying one or more strains of Chlamydia trachomatis in a sample, in which step (iii) involves hybridization with at least one of the following probes:
CHTR-ICG-1: GGAAGAAGCCTGAGAAGGTTTCTGAC (SEQ ID NO 45)
CHTR-ICG-2: GCATTTATATGTAAGAGCAAGCATTCTATTTCA (SEQ ID NO 46)
CHTR-ICG-3: GAGTAGCGTGGTGAGGACGAGA (SEQ ID NO 47)
CHTR-ICG-4: GAGTAGCGCGGTGAGGACGAGA (SEQ ID NO 201)
or with equivalents of the indicated probes, and / or with any probe of SEQ ID NO 123 or 197, providing specific hybridization of said probe with Chlamydia trachomatis.

41. The method of claim 39 for detecting and identifying one or more strains of Chlamydia psittaci in a sample, in which step (iii) involves hybridization with at least the following probe:
CHPS-ICG-1: GGATAACTGTCTTAGGACGGTTTGAC (SEQ ID NO 48)
or with equivalents of the specified probe, and / or with any probe of SEQ ID NO 122, providing specific hybridization of the specified probe with Chlamydia psittaci strains.

42. The method according to claim 1 for determining and identifying in the sample one or more strains of Streptococcus, in which stage (iii) includes hybridization with any probe of SEQ ID NO 145, 146, 147, 148, 149, 150, 151, 152 or 153, providing specific hybridization of the specified probe with Streptococcus strains.

43. The method according to claim 1 for the specific determination and identification in the sample of Chlamydia trachomatis, in which stage (ii) involves amplifying the 16S23S rRNA spacer region or a portion thereof using at least one of the following primers:
CHTR-P1: AAGGTTTCTGACTAGGTTGGGC (SEQ ID NO 69)
CHTR-P2: GGTGAAGTGCTTGCATGGATCT (SEQ ID NO 70)
or equivalents of these primers, while these equivalents, differing in sequence from the above-mentioned primers by replacing one or more nucleotides, still retain the ability to specifically amplify the Chlamydia trachomatis spacer region or a part of it.

44. The method according to claim 1 for the specific determination and identification in a sample of the species Listeria, in which stage (ii) involves amplifying the 16S-23S rRNA spacer region or part thereof using at least one of the following primers:
LIS-P1: ACCTGTGAGTTTTCGTTCTTCTC (SEQ ID NO 71)
LIS-P2: CTATTTGTTCAGTTTTGAGAGGTT (SEQ ID NO 72)
LIS-P3: ATTTTCCGTATCAGCGATGATAC (SEQ ID NO 73)
LIS-P4: ACGAAGTAAAGGTTGTTTTTCT (SEQ ID NO 74)
LIS-P5: GAGAGGTTACTCTCTTTTATGTCAG (SEQ ID NO 75)
LIS-P6: CTTTTATGTCAGATAAAGTATGCAA (SEQ ID NO 202)
LIS-P7: CGTAAAAGGGTATGATTATTTG (SEQ ID NO 203)
or equivalents of these primers, while these equivalents, differing in sequence from the above-mentioned primers by replacing one or more nucleotides, still retain the ability to specifically amplify a spacer region of the Listeria species or a part of it.

45. The method according to claim 1 for the specific determination and identification in a sample of the species Mycobacterium, in which stage (ii) involves amplifying the 16S-23S rRNA spacer region or part thereof using at least one of the following primers:
MYC-P1: TCCCTTGTGGCCTGTGTG (SEQ ID NO 65)
MYC-P2: TCCTTCATCGGCTCTCGA (SEQ ID NO 66)
MYC-P3: GATGCCAAGGCATCCACC (SEQ ID NO 67)
MYC-P4: CCTCCCACGTCCTTCATCG (SEQ ID NO 68)
MYC-P5: CCTGGGTTTGACATGCACAG (SEQ ID NO 192)
or equivalents of these primers, while these equivalents, differing in sequence from the above-mentioned primers by replacing one or more nucleotides, still retain the ability to specifically amplify a spacer region of the Mycobacterium species or part thereof.

46. Composition comprising at least one of the probes or primers defined in paragraphs.1-45 and 51-53.

47. The probe defined in any of paragraphs.1-42 and 51.

48. Primer, as defined in any of paragraphs 43-45 and 52 and 53.

49. A reverse hybridization method comprising any of the probes as defined in claims 1-42 and 51, in which said probes are immobilized at known positions on a solid substrate, more preferably on a membrane strip.

50. A kit for identifying and identifying at least one microorganism or simultaneously identifying and identifying various microorganisms in a sample, including the following components:
(i) if necessary, at least one suitable pair of primers, allowing the amplification of the 16S-23S rRNA spacer region or part thereof;
(ii) at least one of the probes defined in paras. from 1 to 42 and in 51;
(iii) a buffer or components necessary for the preparation of a buffer allowing the hybridization reaction to be carried out between said probes and the polynucleic acids present in the sample or their amplification products;
(iv) the solution or components necessary to prepare the solution, allowing for the washing of the resulting hybrids under appropriate washing conditions;
(v) if necessary, a means for detecting hybrids formed in the preceding hybridization reaction.

51. The method according to claim 1 for determining and identifying in the sample one or more strains of Yersinia enterocolitica, in which stage (iii) involves hybridization with at least one of the following probes:
YEC-ICG-1: GGAAAAGGTACTGCACGTGACTG (SEQ ID NO 198)
YEC-ICG-2: GACAGCTGAAACTTATCCCTCCG (SEQ ID NO 199)
YEC-ICG-3: GCTACCTGTTGATGTAATGAGTCAC (SEQ ID NO 200)
or with equivalents of these probes, and / or with any probe from SEQ ID NO 195 or 196, providing specific hybridization of said probe with Yersinia enterocolitica strains.

52. The method according to claim 1 for the specific determination and identification in a sample of the Brucella species, in which stage (ii) involves amplifying the 16S-23S rRNA spacer region or a portion thereof using at least one of the following primers:
BRU-P1: TCGAGAATTGGAAAGAGGTC (SEQ ID NO 204)
BRU-P2: AAGAGGTCGGATTTATCCG (SEQ ID NO 205)
BRU-P3: TTCGACTGCAAATGCTCG (SEQ ID NO 206)
BRU-P4: TCTTAAAGCCGCATTATGC (SEQ ID NO 207)
or equivalents of these primers, while these equivalents, differing in sequence from the above-mentioned primers by replacing one or more nucleotides, still retain the ability to specifically amplify a spacer region of the Brucella species or part of it.

53. The method according to claim 1 for the specific determination and identification in a sample of the species Yersinia enterocolitica, in which stage (ii) involves amplifying the 16S-23S rRNA spacer region or part thereof using at least one of the following primers:
YEC-P1: CCTAATGATATTGATTCGCG (SEQ ID NO 208)
YEC-P2: ATGACAGGTTAATCCTTACCCC (SEQ ID NO 209)
or equivalents of these primers, while these equivalents, differing in sequence from the above-mentioned primers by replacing one or more nucleotides, still retain the ability to specifically amplify a spacer region of the Yersinia enterocolitica species or a part of it.