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RS60492B1 - Multipleksirani genomski inženjering omogućen pomoću crispr - Google Patents

Multipleksirani genomski inženjering omogućen pomoću crispr

Info

Publication number
RS60492B1
RS60492B1 RS20200793A RSP20200793A RS60492B1 RS 60492 B1 RS60492 B1 RS 60492B1 RS 20200793 A RS20200793 A RS 20200793A RS P20200793 A RSP20200793 A RS P20200793A RS 60492 B1 RS60492 B1 RS 60492B1
Authority
RS
Serbia
Prior art keywords
genome engineering
multiplexed genome
crispr enabled
enabled multiplexed
crispr
Prior art date
Application number
RS20200793A
Other languages
English (en)
Inventor
Ryan T Gill
Andrew Garst
Original Assignee
Univ Colorado Regents
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=53800598&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=RS60492(B1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Colorado Regents filed Critical Univ Colorado Regents
Publication of RS60492B1 publication Critical patent/RS60492B1/sr

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
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    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
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    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
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    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Biotechnology (AREA)
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  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
RS20200793A 2014-02-11 2015-02-11 Multipleksirani genomski inženjering omogućen pomoću crispr RS60492B1 (sr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461938608P 2014-02-11 2014-02-11
EP15749644.9A EP3105328B1 (en) 2014-02-11 2015-02-11 Crispr enabled multiplexed genome engineering
PCT/US2015/015476 WO2015123339A1 (en) 2014-02-11 2015-02-11 Crispr enabled multiplexed genome engineering

Publications (1)

Publication Number Publication Date
RS60492B1 true RS60492B1 (sr) 2020-08-31

Family

ID=53800598

Family Applications (1)

Application Number Title Priority Date Filing Date
RS20200793A RS60492B1 (sr) 2014-02-11 2015-02-11 Multipleksirani genomski inženjering omogućen pomoću crispr

Country Status (22)

Country Link
US (16) US10266849B2 (sr)
EP (4) EP3105328B1 (sr)
JP (3) JP2017505145A (sr)
KR (3) KR102496984B1 (sr)
CN (2) CN106164271B (sr)
AU (2) AU2015217208B2 (sr)
CA (3) CA3075047C (sr)
CY (1) CY1123115T1 (sr)
DE (1) DE212015000061U1 (sr)
DK (1) DK3105328T3 (sr)
ES (2) ES2974229T3 (sr)
GB (1) GB2544382B (sr)
HR (1) HRP20201040T1 (sr)
HU (2) HUE066611T2 (sr)
IL (2) IL246951B (sr)
LT (1) LT3105328T (sr)
MX (1) MX2016010285A (sr)
PL (1) PL3105328T3 (sr)
PT (1) PT3105328T (sr)
RS (1) RS60492B1 (sr)
SI (1) SI3105328T1 (sr)
WO (1) WO2015123339A1 (sr)

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