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KR950026986A - Method for producing N-carbamoyl-D-amino acid using high temperature novel microorganism having heat resistant D-hydantoinase activity - Google Patents

Method for producing N-carbamoyl-D-amino acid using high temperature novel microorganism having heat resistant D-hydantoinase activity Download PDF

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Publication number
KR950026986A
KR950026986A KR1019940005833A KR19940005833A KR950026986A KR 950026986 A KR950026986 A KR 950026986A KR 1019940005833 A KR1019940005833 A KR 1019940005833A KR 19940005833 A KR19940005833 A KR 19940005833A KR 950026986 A KR950026986 A KR 950026986A
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South Korea
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carbamoyl
enzyme
amino acid
producing
bacillus stearothermophilus
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KR1019940005833A
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Korean (ko)
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KR970005487B1 (en
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김학성
이승구
이동철
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조병희
동서석유화학 주식회사
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/02—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amides (3.5.2)
    • C12Y305/02002—Dihydropyrimidinase (3.5.2.2), i.e. hydantoinase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명에서는 약 550℃ 이상의 온도에서 내열성 D-히단토이나제 활성을 갖는 바실러스 스티아로더 모필러스 SD-1을 이용하여 5-치환 히단토인 유도체로부터 N-카르바모일-D-아미노산을 제조하는 방법을 제공한다. 이 방법에서는 바실러스 스티아로더모필러스 SD-1를 균체 자체로서 이용하거나 그로부터 유래된 효소를 이용한다.In the present invention, N-carbamoyl-D-amino acid is prepared from a 5-substituted hydantoin derivative using Bacillus Stiaroder Morphilus SD-1 having heat-resistant D-hydantoinase activity at a temperature of about 550 ° C. or higher. Provide a method. In this method, Bacillus stearothermophilus SD-1 is used as the cell itself or an enzyme derived therefrom.

Description

내열성 D-히단토이나제 활성을 갖는 고온성 신규 미생물을 이용한 N-카르바모일-D-아미노산의 제조 방법Method for producing N-carbamoyl-D-amino acid using high temperature novel microorganism having heat resistant D-hydantoinase activity

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 마그네슘 알루미늄 실리케이트 담체에 고정화시킨 D-히단토이나제 효소를 반복 사용하는 경우 산물의 생산성을 나타내는 그래프임.1 is a graph showing the productivity of the product when repeated use of the D- hydantoinase enzyme immobilized on magnesium aluminum silicate carrier.

Claims (11)

5-치환 히단토인 유도체를 약 550℃ 이상의 온도에서 고온성 바실러스 스티아로더모필러스(Bacillus stearot hermophilus)SD-1의 효소와 반응시키는 것을 특징으로 하는 대응하는 N-카르바모일-D-아미노산의 제조 방법.Reaction of the corresponding N-carbamoyl-D-amino acid, characterized in that the 5-substituted hydantoin derivative is reacted with an enzyme of Bacillus stearot hermophilus SD-1 at a temperature of about 550 ° C. or higher. Manufacturing method. 제1항에 있어서, 상기 효소는 바실러스 스티아로더모필러스 DS-1 균체 자체 또는 그의 변형물인 것을 이용하는 방법.The method according to claim 1, wherein the enzyme is Bacillus stearothermophilus DS-1 cells themselves or a modification thereof. 제2항에 있어서, 상기 균체 또는 그의 변형물은 고정화된 것인 방법.The method of claim 2, wherein the cells or variants thereof are immobilized. 제1항에 있어서, 상기 효소는 바실러스 스티아로더모필러스 SD-1 유래의 추출물, 부분 정제, 또는 완전 정제된 효소인 것인 방법.The method of claim 1, wherein the enzyme is an extract, partially purified, or fully purified enzyme from Bacillus stearothermophilus SD-1. 제4항에 있어서, 상기 추출물, 부분 정제, 또는 완전 정제된 효소는 담체에 고정화된 것인 방법.The method of claim 4, wherein the extract, partially purified, or fully purified enzyme is immobilized on a carrier. 제5항에 있어서, 상기 담체는 마그네슘 알루미늄 실리게이트인 것인 방법.The method of claim 5, wherein the carrier is magnesium aluminum silicate. 제1항 내지 6항에 있어서, 상기 효소는 히단토인 가수 분해 효소(히단토이나제)인 것인 방법.The method of claim 1, wherein the enzyme is hydantoin hydrolase (hydantoinase). 제1항에 있어서, 5-치환 히단토인 유도체는 DL-5-(파라-히드록시페닐)히단토인, DL-5-(2'-메틸티오에틸렌)히단토인, DL-5-페닐히단토인, 또는 DL-5-이소프로필히단토인인 것인 방법.The method of claim 1, wherein the 5-substituted hydantoin derivative is DL-5- (para-hydroxyphenyl) hydantoin, DL-5- (2'-methylthioethylene) hydantoin, DL-5-phenylhydantoin, Or DL-5-isopropylhydantoin. 제1항에 있어서, N-카르바모일-D-아미노산은 N-카르바모일-D-파라-히드록시페닐 글리신, N-카르바모일-D-메티오닌, N-카르바모일-D-페닐글리-신, 또는 N-카르바모일-D-발린인 것인 방법.The N-carbamoyl-D-amino acid of claim 1, wherein the N-carbamoyl-D-amino acid is N-carbamoyl-D-para-hydroxyphenyl glycine, N-carbamoyl-D-methionine, N-carbamoyl-D-phenyl Glycine, or N-carbamoyl-D-valine. 바실러스 스티아로더모필러스 SD-1 유래의 추출물, 부분 정제, 또는 완전 정제된 효소를 마그네슘 알루미늄 실리게이트에 고정화시키는 것을 특징으로 하는 고정화 효소의 제조 방법.A method for producing an immobilized enzyme, characterized by immobilizing an extract, partial purified, or fully purified enzyme derived from Bacillus stearothermophilus SD-1 to magnesium aluminum silicate. 마그네슘 알루미늄 실리케이트에 고정화된 것이 특징인 바실러스 스티아로더모필러스 SD-1의 효소.Enzyme of Bacillus stearothermophilus SD-1, characterized by immobilization on magnesium aluminum silicate. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940005833A 1994-03-23 1994-03-23 Process for the preparation of n-carbamoyl-d-amino acid using novel thermophilic microorganism having heat-resistant d-hydantoinase activity KR970005487B1 (en)

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KR1019940005833A KR970005487B1 (en) 1994-03-23 1994-03-23 Process for the preparation of n-carbamoyl-d-amino acid using novel thermophilic microorganism having heat-resistant d-hydantoinase activity

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KR1019940005833A KR970005487B1 (en) 1994-03-23 1994-03-23 Process for the preparation of n-carbamoyl-d-amino acid using novel thermophilic microorganism having heat-resistant d-hydantoinase activity

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KR970005487B1 KR970005487B1 (en) 1997-04-16

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