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KR930018032A - Method for preparing L-ornithine using complex enzyme - Google Patents

Method for preparing L-ornithine using complex enzyme Download PDF

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Publication number
KR930018032A
KR930018032A KR1019920001690A KR920001690A KR930018032A KR 930018032 A KR930018032 A KR 930018032A KR 1019920001690 A KR1019920001690 A KR 1019920001690A KR 920001690 A KR920001690 A KR 920001690A KR 930018032 A KR930018032 A KR 930018032A
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KR
South Korea
Prior art keywords
ornithine
complex enzyme
complex
preparing
arginase
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Application number
KR1019920001690A
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Korean (ko)
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KR950009319B1 (en
Inventor
박영훈
안경섭
김익환
Original Assignee
박원희
한국과학기술연구원
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Priority to KR1019920001690A priority Critical patent/KR950009319B1/en
Publication of KR930018032A publication Critical patent/KR930018032A/en
Application granted granted Critical
Publication of KR950009319B1 publication Critical patent/KR950009319B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 복합요소를 이용한 L-오르니친의 제조방법에 관한것으로서, 특히 아르기나제와 우레아제의 복합 효소를 엠버라이트, 키틴, 셀라이트, 글리세릴,-CGP중에서 선택된 어느하나 또는 그 이상으로 이루어진 흡착성 담체에 복합고정하여 온도 25∼40℃, pH7∼8의 반응조건에서 실시하므로써 L-아르기닌으로 부터 L-오르니친을 짧은 시간에 고수율로 제조하는 방법에 관한 것이다.The present invention relates to a method for producing L-ornithine using a complex element, in particular the complex enzyme of arginase and urease consisting of any one or more selected from amberlite, chitin, celite, glyceryl, -CGP The present invention relates to a method for producing L-ornithine from L-arginine in high yield in a short time by performing complex fixation on an adsorptive carrier at a temperature of 25 to 40 ° C. and a pH of 7 to 8.

Description

복합효소를 이용한 L-오르니친의 제조방법Method for preparing L-ornithine using complex enzyme

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 우레아제(urease)를 아르기나제(arginase)와 함께 사용했을때 반응시간에 따른 L-오르니친(L-Ornithine)의 수율을 나타낸 도면, 제2도는 우레아제(urease)와 아르기나제(arginase)를 동시 고정화시킨후 연속생산 반응기에서의 L-오르니친(L-Ornithine)의 수율을 나타낸 도면이다.1 is a view showing the yield of L- ornithine (L-Ornithine) according to the reaction time when urease (urease) is used with arginase, Figure 2 is a urease (arase) and arginase ( Figure 2 shows the yield of L-Ornithine in a continuous production reactor after co-immobilization of arginase).

Claims (3)

L-아르기닌을 복합효소를 이용하여 효소반응시켜 L-오르니친을 제조함에 있어서, 아르기나제와 함께 우레아제를 동시에 복합효소로 사용하여 온도 25∼40℃, 그리고 pH 7∼8의 반응조건에서 실시하는 것을 특징으로 하는 L-오르니친의 제조방법.In the preparation of L-ornithine by enzymatic reaction of L-arginine with a complex enzyme, it was carried out under the reaction conditions of 25-40 ° C. and pH 7-8 using urease as a complex enzyme simultaneously with arginase. Process for producing L-ornithine, characterized in that. 제1항에 있어서, 상기 아르기나제와 우레아제는 흡착성 담체에 복합 고정화하여 사용하는 것을 특징으로하는 L-오르니친의 제조방법.The method of claim 1, wherein the arginase and urease are used by complex immobilization on an adsorptive carrier. 제2항에 있어서, 상기 흡착성 담체로는 앰버라이트, 키틴, 셀라이트, 글리세릴-CPG 중에서 선택된 어느 하나 또는 그 이상을 사용하는 것을 특징으로 하는 L-오르니친의 제조방법.The method of claim 2, wherein the adsorptive carrier is one or more selected from amberlite, chitin, celite, and glyceryl-CPG. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920001690A 1992-02-06 1992-02-06 Preparation method of l-ornithine by using enzyme complex KR950009319B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920001690A KR950009319B1 (en) 1992-02-06 1992-02-06 Preparation method of l-ornithine by using enzyme complex

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920001690A KR950009319B1 (en) 1992-02-06 1992-02-06 Preparation method of l-ornithine by using enzyme complex

Publications (2)

Publication Number Publication Date
KR930018032A true KR930018032A (en) 1993-09-21
KR950009319B1 KR950009319B1 (en) 1995-08-19

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KR1019920001690A KR950009319B1 (en) 1992-02-06 1992-02-06 Preparation method of l-ornithine by using enzyme complex

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105175275B (en) * 2015-07-01 2017-03-01 山东民强生物科技股份有限公司 A kind of isolation and purification method of L ornithine

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