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KR920701454A - 피타아제의 식물내 발현 - Google Patents

피타아제의 식물내 발현

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KR920701454A
KR920701454A KR1019910701654A KR910701654A KR920701454A KR 920701454 A KR920701454 A KR 920701454A KR 1019910701654 A KR1019910701654 A KR 1019910701654A KR 910701654 A KR910701654 A KR 910701654A KR 920701454 A KR920701454 A KR 920701454A
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phytase
plant
dna sequence
feed
directing
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KR1019910701654A
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KR100225087B1 (ko
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펜 얀
희케마 안드레아스
크리스치안 시으몬스 페터
리이트벨트 크리은
코르넬리스 베르뵈르트 토이니스
예.예. 반 오오엔 알베르트
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원본미기재
기스트-브로카데스 나암로제 베노오트하프
모겐 인터내쇼날 나암로제 베노오트하프
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    • C12Y301/03026—4-Phytase (3.1.3.26), i.e. 6-phytase

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Abstract

내용 없음

Description

피타아제의 식물내 발현
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 파타아제 cDNA의 클로닝을 위한 계획도, 제2도는 피타아제 cDNA 단편의 번역된 영역의 뉴클레오티드서열 및 피타아제 단백질의 유도 아미노산 서열; 성숙한 피타아제 단백질의 개시점은 위치+1로 표시됨, 제3도는 이진 벡터 pMOG29.

Claims (21)

  1. 증가된 양의 피타아제를 함유하는 트랜스제닉 식물 또는 식물기관의 생산 방법에 있어서, 식물숙주내에 있어 피타아제의 발현을 지시할수 있고 도움이 되는 조건하에서 형질전환된 식물을 성장시킬수 있는 조절서열과 작동적으로 연결된 피타아제를 코드하는 DNA 서열을 포함하고 있는 발현 구성체로 식물 숙주를 형질전환하여, 피타아제 코드 DNA 서열이 발현되게 하는 것을 특징으로 하는 방법.
  2. 제1항에 있어서, 발현 구성체는 피타아제의 구성 발현을 지시할수 있는 조절서열을 갖고 있는 것을 특징으로 하는 방법.
  3. 제1항에 있어서, 발현 구성체는 피타아제의 조직특이 발현을 지시할수 있는 것을 특징으로 하는 방법.
  4. 제1항에 있어서, 피타아제코드 DNA 서열을 미생물원으로 부터 얻는 것을 특징으로 하는 방법.
  5. 제4항에 있어서, 피타아제코드 DNA 서열을 선조상 곰팡이원으로부터 얻는 것을 특징으로 하는 방법.
  6. 제5항에 있어서, 피타아제 코드 DNA 서열을 아스페르길러스 원으로부터 얻는 것을 특징으로 하는 방법.
  7. 제6항에 있어서, 피타아제 코드 DNA 서열을 아스페르길러스 피큐움, 아스페르길러스, 니게르, 아스페르 길러스 아와모리, 아스페르길러스 니둘란스로 구성된 군으로부터 선택된 아스페르길러스 원으로부터 얻는 것을 특징으로 하는 방법.
  8. 피타아제를 코드하는 DNA 서열이 피타아제의 구성발현을 지시할수 있는 조절서열에 작동적으로 연결되어 있는 것을 특징으로 하는 발현 구성체.
  9. 벡터가 제8항에 기제된 발현 구성체를 내포하고 있는 것을 특징으로 하는 벡터.
  10. 균주가 제8항에 의한 벡터를 내포하는 것을 특징으로 하는 세균주.
  11. 피타아제를 코드하는 DNA 서열이 피타아제의 조직 특이발현을 지시할수 있는 조절 서열에 작동적으로 연결되어 있는 것을 특징으로 하는 발현 구성체.
  12. 벡터가 제11항에 기재된 발현 구성체를 내포하고 있는 것을 특징으로 하는 벡터.
  13. 균주가 제12항에 기재된 벡터를 내포하는 것을 특징으로 하는 세균주.
  14. 식물 또는 식물기관이, 식물 숙주내 피타아제의 발편을 지시할수 있는 조절서열에 작동적으로 연결된, 피타아제를 코드하는 DNA 서열을 내포하고 있는 발현 구성체를 내포하고 있는 것을 특징으로 하는 트랜스제닉 식물 또는 식물기관.
  15. 피틴산염을 함유하는 기질에 제1항 내지 7항중의 어느한항의 방법에 따라 생산된 피타아제를 가하는 것을 특징으로 하는 피틴산염을이노시톨 및 무기인산염으로 전환시키는 방법.
  16. 사료는 제1항 내지 7항중의 어느 한항의 방법에 따라 생산된 피타아제를 포함하는 것을 특징으로 하는 동물용 사료조성물.
  17. 제16항에 있어서, 사료는 제1항 내지 7항중의 어느 한항의 방법에 따라 생산된 피타아제를 함유하는 트랜스제닉 식물 또는 식물 기관을 포함하는 것을 특징으로 하는 사료 조성물.
  18. 사료를 소화하는 동물의 성장을 촉진하기에 유효한 양의, 제16항에 의한 사료로 구성되어 있는 먹이를 동물에 공급하는 것을 특징으로 하는 동물의 성장을 촉진하는 방법.
  19. 사료에 함유된 피틴산염을 이노시톨 및 무기인산염으로 변환시키기에 유효한 양의 제16항에 의한 사료로 구성되어 있는 먹이를 동물에 공급하는 것을 특징으로 하는 동물배설물중의 피탄산염 수준을 감소시키는 방법.
  20. a)피타아제를 코드하는 DNA 서열이 발현되도록, 식물숙주내에 있어 증가된 양의 피타아제의 발편을 지시할수있고 도움이 되는 조건하에서 형질전환된 식물을 성장시킬수 있는 조절서열과 작동적으로 연결된 피타아제를 코드하는 DNA 서열을 포함하고 있는 발편 구성체로 식물숙주를 형질전환하며; b)피타아제를 트랜스제닉 식물조직으로 추출하는 것을 특징으로 하는 피타아제의 제조방법.
  21. 제1항 내지 제7항 및 제21항중의 어느 한항의 방법에 따라 제조된 피타아제의 사료 또는 동물먹이로서의 이용.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910701654A 1990-03-23 1990-09-21 피타아제의 식물내 발현 KR100225087B1 (ko)

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Application Number Priority Date Filing Date Title
US49856190A 1990-03-23 1990-03-23
US498561 1990-03-23
US498,561 1990-03-23
US58676590A 1990-09-21 1990-09-21
US586,765 1990-09-21
US586765 1990-09-21

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KR920701454A true KR920701454A (ko) 1992-08-11
KR100225087B1 KR100225087B1 (ko) 1999-10-15

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US (1) US5770413A (ko)
EP (1) EP0449375B1 (ko)
JP (1) JP3471795B2 (ko)
KR (1) KR100225087B1 (ko)
AT (1) ATE328093T1 (ko)
AU (1) AU632941B2 (ko)
CA (1) CA2056396C (ko)
DE (1) DE69133533T2 (ko)
ES (1) ES2267092T3 (ko)
FI (1) FI915477A0 (ko)
HU (1) HU215164B (ko)
IL (1) IL97646A (ko)
NZ (1) NZ237550A (ko)
PT (1) PT97111B (ko)
RU (1) RU2128228C1 (ko)
WO (1) WO1991014782A1 (ko)

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