KR20190116693A - Cosmetic composition for improving skin condition comprising human epidermal growth factor, peptides and functional materials - Google Patents

Abstract

Disclosed herein is a cosmetic composition for improving skin condition comprising human epidermal growth factors, peptides and functional materials excellent in skin condition improving effects. In one aspect of the present invention, the cell growth factor-containing composition with improved stability includes glycerin at a high concentration and a combination of a cellulose derivative and a hyaluronic acid salt, and thus can stably contain various kinds of cell growth factors and various bio-derived functional materials in addition to cell growth factors such as peptides. In addition, the composition is not sticky when applied to the skin, and has excellent skin absorption, feeling during use and skin condition improvement effects.

Description

Cosmetic composition for improving skin condition containing human epidermal growth factor, peptide and functional material {COSMETIC COMPOSITION FOR IMPROVING SKIN CONDITION COMPRISING HUMAN EPIDERMAL GROWTH FACTOR, PEPTIDES AND FUNCTIONAL MATERIALS}

Disclosed herein is a cosmetic composition for improving skin condition containing human epidermal growth factor, peptide, and a functional material excellent in skin condition improving effect.

Recently, with the development of life science technology, there is increasing interest in the therapeutic effect of the biofactors present in vivo. In particular, some cell growth factors are known to have excellent skin regeneration effects, and growth factors such as human epidermal growth factor and fibroblast growth factor are listed in the International Cosmetics Raw Materials List (ICID) of the American Cosmetic Association (CTFA). In addition, it has been approved as a cosmetic raw material by Korea Food and Drug Administration and is used as a cosmetic raw material at home and abroad. It is mass-produced through various genetic engineering techniques. However, the cell growth factor has a problem in that degeneration is easily caused by minute environmental changes such as temperature, pressure, pH (pH), ion concentration, etc., and the biological activity is rapidly lost.

For this reason, attempts to secure thermal stability of cell growth factors (Korean Patent Publication No. 10-2017-0127704) continue, and this patent relates to an EGF heat stabilizing composition, which is applied when applied to a human body as a cosmetic composition. After the sticky there was a problem that many users feel uncomfortable. In addition, Korean Patent 10-0748390B1 relates to a quasi-drug sustained-release film formulation for wound treatment containing EGF, which contains L-histidine and an antioxidant as a heat stabilizer and glycerin as a plasticizer so that these formulations cannot be used as cosmetics. There is this.

KR 10-2017-0127704 A KR 10-0748390 B1

In one aspect, it is an object of the present invention to provide a cosmetic composition that can stably contain various kinds of cell growth factors.

In one aspect, an object of the present invention is to provide a cosmetic composition that can stably contain various kinds of biologically derived functional peptides and the like.

In one aspect, an object of the present invention is to provide a cosmetic composition excellent in feeling even if the cell growth factor and biologically derived functional material (peptide, etc.) in various concentrations.

In one aspect, an object of the present invention is to improve the skin absorption rate of the cell growth factor and bio-derived functional material (peptide, etc.) contained in the cosmetic composition.

In one aspect, an object of the present invention is to provide a composition excellent in improving skin condition.

In one aspect, the invention provides a cell growth factor; Peptides; Nucleotides; vitamin; glycerin; Cellulose derivatives; And it comprises a hyaluronic acid salt, to improve the stability of each component, to provide a cosmetic composition excellent in improving the skin condition with high skin absorption.

According to one aspect of the present invention, the cell growth factor-containing composition having improved stability includes a high concentration of glycerin and a combination of a cellulose derivative and a hyaluronic acid salt, thereby stably containing various kinds of cell growth factors, and a peptide. Various biologically-derived functional substances such as lysates can be contained biologically and physicochemically. In addition, the composition is not sticky when applied to the skin, the feeling of use is excellent, the skin absorption is excellent, and the skin condition improvement effect is excellent.

Figure 1 is one aspect of the present invention after storing the cosmetic composition containing glycerin at different concentrations (15, 30, 45% by weight) for 30 days at 30 ℃, using cell growth factors, peptides and whitening functionality using HPLC As a result of analyzing the stability of the material, it shows the stability (%) of the epidermal growth factor after 15 days, 30 days.
Figure 2 is a result of analyzing the skin wrinkle amount improvement rate (Fig. 2a) and the maximum skin wrinkle improvement rate (Fig. 2b) before and after the use of the composition, after two weeks, four weeks after the use of one aspect of the present invention.
3 is a result photograph (antera 3D) of the skin wrinkle improvement effect showing a change in the skin condition before and after the use of the composition and after 2 and 4 weeks of use of one aspect of the present invention.
Figure 4 is a result of analyzing the skin brightness improvement rate before and after the use of the composition, after 2 weeks, after 4 weeks of use of one aspect of the present invention.

Hereinafter, the present invention will be described in detail.

In one aspect, the invention provides a cell growth factor; Peptides; Nucleotides; vitamin; glycerin; Cellulose derivatives; And a hyaluronic acid salt, wherein the composition contains improved cell growth factor.

In addition, the present invention, cell growth factor; Peptides; Nucleotides; vitamin; glycerin; Cellulose derivatives; And a hyaluronic acid salt, wherein the composition contains a cell growth factor having improved skin absorption.

In the present specification, the stability may include biological activity and physicochemical stability, and the improvement in stability may include the improvement of precipitation, formulation separation, and alteration of the active ingredient contained in the composition by external environmental factors such as temperature. Can mean.

Epithelial growth factor and the like, despite the excellent skin improvement effect, has been pointed out that the problem is unstable at room temperature and its utility as a cosmetic composition is poor.

On the other hand, the composition is a variety of cell growth factors, not only at low temperatures (about 0 ~ 5 ℃), but also at room temperature (20 ~ 30 ℃) and high temperature (30 ℃) does not occur biological and physicochemical denaturation such as cell growth factors Stable.

Preferably, the cell growth factor contained in the composition is epidermal growth factor (EGF), cosmetic ingredient name: HS-oligopeptide-1); And fibroblast growth factor (FGF), cosmetic ingredient name: HS-polypeptide-1. In view of the above, the composition includes epithelial cell growth factor and fibroblast growth factor among cell growth factors, thereby obtaining better skin condition improvement effects, such as wrinkle improvement, whitening, moisturizing, or skin tone improvement. Can be.

In one aspect, the composition, including a combination of glycerin, cellulose derivatives and hyaluronic acid salts, it is possible to improve the stability of the various active ingredients contained in the composition and the user's feeling of use.

In addition, in the above aspect, the cellulose derivative may include one or more selected from the group consisting of ethyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, and hydroxyethyl cellulose, preferably hydroxyethyl cellulose It may include. The composition may include a cellulose derivative, thereby improving the feeling of use by controlling the viscosity of the cosmetic compared to the case containing only glycerin.

The vitamin may include vitamin B or a derivative thereof, and may include, for example, niacinamide. Niacinamide may be included in an amount of 0.5 to 5% by weight, preferably 2-5% by weight, based on the total weight of the composition.

In addition, the nucleotide of the composition may include adenosine, the adenosine may be included in 0.01 to 0.1% by weight, preferably, 0.01 to 0.06% by weight.

In addition, the composition may include sodium hyaluronate as a hyaluronic acid salt. The composition includes sodium hyaluronate, so that the user's sensitivity can be significantly improved compared to the case of containing only glycerin.

In one aspect, the cell growth factor may comprise human cell growth factor. For example, the cell growth factor may include human epidermal cell growth factor, or human fibroblast growth factor.

In addition, in the above aspect, the fibroblast growth factor may comprise an acidic or basic fibroblast growth factor.

The glycerin may be included in 30% by weight or more and 60% by weight or less based on the total weight of the composition. The glycerin is preferably 30 to 50% by weight, more preferably 40 to 50% by weight, most preferably, may be included in 43 to 48% by weight.

In addition, the composition may include 0.1 to 100 ppm (w / w) of the cell growth factor, based on the total weight of the composition.

Specifically, the cell growth factor may be included preferably 0.1 to 30 ppm, more preferably 0.2 to 10 ppm (w / w).

In one embodiment, each of the epidermal growth factor and fibroblast growth factor may be included in 6 ~ 14ppm (w / w), preferably 8 ~ 12ppm (w / w). Within the content range, the epidermal growth factor and fibroblast growth factor may be included in a weight ratio of 0.8 to 1.2: 0.8 to 1.2, and most preferably in a ratio of about 1: 1.

In addition, the cellulose derivative may be included in an amount of 0.05 wt% or more and less than 0.2 wt% based on the total weight of the composition, preferably, 0.05 to 0.15 wt%, more preferably 0.08 to 0.12 wt%.

In addition, when a numerical value is represented by the range "X-Y" in this specification, unless there is particular notice, it interprets as X or more and Y or less.

In addition, the hyaluronic acid salt, based on the total weight of the composition may be included in more than 0.05% by weight less than 0.2% by weight, preferably, 0.05 to 0.15% by weight, more preferably 0.08 to 0.12% by weight. have.

In addition to the above components, the composition may further include a functional material, in particular, a bio-derived functional material or a complex including a bio-derived functional material (a material synthesized using a bio-derived functional material). The biologically derived functional substance may include peptides, amino acids, and the like. The peptides may include acetylhexapeptide-8, palmitoylpentapeptide-4, cappertripeptide-1, and the like, but are not limited thereto.

The peptides may be included in an amount of 0.0005% by weight to 0.4% by weight based on the total weight of the composition, and specifically, the composition may include acetylhexapeptide-8, cappertrippeptide-1, and palmi as peptides. Or may be one or more of tonypentapeptide-4.

When the composition includes all of acetylhexapeptide-8, cappertrippeptide-1, and palmitoylpentapeptide-4, each of acetylhexapeptide-8, cappertree peptide-1, and palmitoylpentapeptide-4 is a composition. Based on the total weight, it may be included in 0.05 to 0.15% by weight, preferably, 0.08 to 0.12% by weight. The composition may further include amino acids, specifically, arginine, glycine, alanine, valine, leucine, isoleucine, phenylalanine, tyrosine, tryptophan, and the like. The composition may preferably further include arginine as amino acids. The arginine may be included in an amount of 0.01 to 0.1% by weight, preferably 0.01 to 0.07% by weight, and more preferably 0.03 to 0.05% by weight, based on the total weight of the composition.

The functional material may further include taurine, and the taurine may be included in an amount of 0.1 to 3% by weight, preferably 0.5 to 1.5% by weight.

In addition, the composition, in order to improve the stability of the components contained in the composition, may further include an acidic component, for example, may further include a citric acid. The citric acid may be included in an amount of 0.005% to 0.02% (w / v) based on the total weight of the composition, and more preferably 0.008% to 0.015%.

In addition, the composition, for improving wrinkles; For enhancing skin elasticity; For skin whitening; For pore reduction; For improving skin troubles; Or a skin moisturizing composition.

In addition, the composition may include a cosmetic composition.

The wrinkle improvement may include improving not only the amount of wrinkles, but also the individual wrinkle depths.

The cosmetic composition may be formulated as a cream, essence, gel, makeup base, lotion, skin, essence and the like.

The cosmetic composition may be for a moderate skin type of various skin types (dry, moderate, neutral, moderate to oily, oily).

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are provided only for the purpose of illustration in order to facilitate understanding of the present invention, the scope and scope of the present invention is not limited thereto.

Preparation Example Preparation of Cosmetic Composition

To the cosmetic composition was prepared in the amount described in Table 1. Specifically, glycerin, sodium hyuronate, hydroxyethyl cellulose, adenosine and niacinamide are dissolved and mixed in purified water at 70 ° C., cooled to 30 ° C., and then the remaining raw materials are added and mixed to obtain the compositions A, B, C was prepared.

Raw material name A B C Purified water Remaining amount (to 100%) Remaining amount (to 100%) Remaining amount (to 100%) glycerin 45% 45% 45% Niacinamide 2% 2% 2% 1,2-hexanediol 2% 2% 2% Taurine One% One% One% Caprylyl Glycol 0.5% 0.5% 0.5% Sodium hyaluronate 0.2% 0.1% - Hydroxyethyl cellulose - 0.1% 0.2% Acetylhexapeptide-8 0.1% 0.1% 0.1% Coppertripeptide-1 0.1% 0.1% 0.1% Palmitoylpentapeptide-4 0.1% 0.1% 0.1% Arginine 0.04% 0.04% 0.04% Adenosine 0.04% 0.04% 0.04% Citrix Acid 0.01% 0.01% 0.01% SH-oligopeptide-1 0.001% 0.001% 0.001% SH-Polypeptide-1 0.001% 0.001% 0.001%

Example 1 Usability Evaluation

Example 1-1 Candidate Composition Selection

Ten women were evaluated for the feeling of use on the cosmetic composition A, B, C prepared in Example 1. The subjects were three in their thirties, three in their 40s, and four in their 50s.

The test subjects scored 5 points for "very good" (very satisfied), 4 points for "yes (satisfied)", 3 points for "normal (average)", and 2 points for "not satisfied" for each item. In Table 2, the average score of one point of "not at all (very dissatisfied)" was added to each item and divided by the number of subjects.

division A B C Feeling when applying for the first time 3.4 4.2 4.7 absorptivity 4.2 4.6 2.8 Sticky after use 3.1 4.8 4.9 Moist after use 4.1 4.4 3.0

As a result, a composition B having 0.1% by weight of sodium hyaluronate and hydroxyethyl cellulose, respectively, was selected as a candidate composition having superior feeling in use, absorption, and the like compared to other compositions.

[Example 1-2] Evaluation of Stability of Functional Components in Cosmetic Compositions According to Glycerin Concentration

Peptides, whitening functional materials and growth factors (epithelial cell growth factor and fibroblasts) in cosmetic compositions containing different concentrations of glycerin (15.30, 45% by weight) in consideration of the properties of the cosmetic composition stored and used at room temperature Growth factor) was analyzed using high performance liquid chromatography (HPLC) using a Water XBridge C18 column.

Each cosmetic composition was stored at 30 ° C. to extract functional ingredients at 15-day intervals, and then acetonitrile was acetonitrile at a rate of 1 mL / min using distilled water solution and acetonitrile containing 0.1% trifluoroacetic acid using HPLC. Reels were analyzed with a linear gradient from 0% to 70%. Cosmetic compositions containing different concentrations of glycerin (15.30, 45% by weight) were analyzed by HPLC to determine the area of the peaks of the functional components on the peaks of the functional components on day 0 (the first production date) after 15 and 30 days. Table 3 shows the results calculated by dividing by the area for the percentage.

15% by weight glycerin Glycerin 30% by weight 45% by weight glycerin Elapsed time 15 days later 30 days later 15 days later 30 days later 15 days later 30 days later Niacinamide 98.03 98.06 98.01 97.97 98.38 98.37 Coppertripeptide-1 97.92 96.22 98.01 97.98 99.16 99.06 Adenosine 98.39 97.73 98.90 98.87 99.36 99.33 Acetylhexapeptide-8 98.95 97.18 97.92 97.93 99.95 99.93 Epithelial cell growth factor 69.09 53.40 79.67 62.14 98.85 97.45 Fibroblast growth factor 96.58 96.81 97.90 96.69 99.53 99.60 Palmitoyl Peptide Peptide-4 95.31 94.98 96.72 96.69 99.73 99.99

 As a result, at 30 ° C., epithelial cell growth factor was more stable than cosmetic composition containing high concentration of glycerin at low concentration of glycerin at about 30% after 15 days and at least about 45% after 30 days. Maintained.

In addition, other components (fibroblast growth factor, peptide, adenosine, niacinamide, etc.) also, when the content of glycerin exceeds 40% by weight, the stability was further improved.

Example 1-3 Evaluation of Use Sensitivity of Composition B

A questionnaire evaluation about subjective use sensibility was carried out on 22 women who were subjected to human skin clinical trials on the cosmetic composition B selected in Example 1-1.

The subjects of evaluation were eight in their thirties, nine in their forties, and five in their fifties.After applying cosmetic composition B twice a day for four weeks, the applicability, absorption, stickiness, fragrance, and overall feeling of use of the provided cosmetic composition B Investigate. The subjects were evaluated 5 points for "very satisfied", 4 points for "yes (satisfied)", 3 points for "normal (average)", and 2 points for "not satisfied" for each question. The average score divided by the number of items that were evaluated as "not at all (very dissatisfied)" by item and divided by the number of subjects is shown in Table 4 by the age and skin type.

division Application absorptivity Sticky incense Overall feeling All (22 people) Average 4.32 4.23 3.73 3.09 4.23 Deviation 0.72 0.92 0.94 1.02 0.87 age 30 spaces
(8 people) Average 4.25 4.00 3.25 3.00 4.00 Deviation 0.71 0.76 0.89 0.53 0.76 40 spaces
(9 people) Average 4.33 4.33 3.89 2.67 4.11 Deviation 0.87 1.12 0.93 1.32 1.05 50 spaces
(5 people) Average 4.40 4.40 4.22 4.00 4.80 Deviation 0.55 0.89 0.89 0.00 0.45 Skin type inattention
(5 people) Average 4.40 4.40 3.20 2.80 4.00 Deviation 0.55 0.89 1.10 0.45 1.00 Medium dryness (6 people) Average 4.00 4.00 3.67 3.33 3.83 Deviation 0.89 0.89 1.03 0.52 1.17 neutrality
(7 people) Average 4.26 4.29 4.14 2.71 4.43 Deviation 0.76 1.11 0.69 1.38 0.53 Stopability
(3 people) Average 4.67 4.67 4.00 3.67 4.67 Deviation 0.58 1.00 1.00 1.15 0.58 Intelligence
(1 person) Average 5.00 5.00 4.00 4.00 5.00 Deviation 0.00 0.00 0.00 0.00 0.00

As a result, in the questions about applicability, absorptivity, and overall feeling of use, there was a tendency that the higher the age, the higher the satisfaction. The overall feeling of use for the cosmetic composition B showed a satisfaction level of 4.0 or more in all ages, and particularly showed 4.80 having the highest satisfaction level in the fifties.

Example 2 Evaluation of Skin Condition Improvement Effect of Composition B

After using cosmetic composition B twice a day for 4 weeks, the skin moisturizing effect, skin texture improvement, skin brightness improvement effect, pore reduction effect, skin elasticity improvement effect, wrinkle improvement effect, skin regeneration effect, purchase motivation Was investigated. The subjects were evaluated 5 points for "very satisfied", 4 points for "yes (satisfied)", 3 points for "normal (average)", and 2 points for "not satisfied" for each question. The average scores and the deviations divided by the number of items and the scores of "not at all (very dissatisfied)" for each item are shown in Table 5 by age and skin type.

TABLE 5

As a result, it was confirmed that there is an effect of improving the skin condition evenly in all ages of the evaluator using the cosmetic composition B, and it was evaluated that there is a willingness to purchase and use continuously in all ages and all skin types.

Example 3 Confirming the Wrinkle Improvement Effect of Composition B

One week prior to the start of the trial, the use of therapeutic agents, cosmetics, and quasi-drugs for the purpose of improving skin, which may affect the evaluation results, was prohibited, and medical treatment and massage procedures were also prohibited. The subjects were provided with the cosmetic composition B for evaluation, and after washing face in the morning and evening, a certain amount was spread on the face and patted by hand (2 times / day / person). After the morning wash, the cosmetic composition B is completely absorbed by the skin and then an additional product is used. After the evening wash, only the cosmetic composition B is used without using any products such as skins and lotions. In order to confirm the skin wrinkle improvement effect of the cosmetic composition B, the equipment (skin 3D measuring instrument, Antera 3D) was used before (first), after 2 weeks (second), and after 4 weeks (third). Table 6 shows the results of analyzing the amount and depth of wrinkles in the skin. The significance of the liver before and after the use of the cosmetic composition B was confirmed using the SPSS (Ver. 19) program ( P <0.05, paired t-test), and the wrinkle improvement rate (%) compared to before use was calculated by substituting the following formula. Is shown in FIG. 2.

[Equation 1]

% Improvement = (Measurement after use-Measurement before use) / Measurement before use X 100 (%)

Before use 2 weeks later 4 weeks later Wrinkle amount (mm) Average 12.11 11.44 11.26 ^* Standard Deviation 2.79 2.03 2.06 Maximum Depth of Wrinkles (mm) Average 0.14 0.13 0.13 Standard Deviation 0.04 0.04 0.04

^* P <0.05, number of subjects: 22

As a result, in the subject using the composition B, the wrinkle amount improvement rate of 5.54% after 2 weeks and 7.02% after 4 weeks of use was compared with before use. After 4 weeks of use, the amount of wrinkles was reduced to a statistically significant level (P <0.05, paired t-test). The maximum wrinkle depth measurement also showed a maximum depth improvement of 7.14% of the wrinkles after 2 and 4 weeks of use in the subjects using the cosmetic composition B.

Example 4 Confirmation of Skin Whitening Effect of Composition B

Preparation method before the experiment of the test subject and the use of the cosmetic composition B was carried out in the same manner as in Example 5. Spectrophotometer was used before (first), after 2 weeks (second), and after 4 weeks (3rd) in the subject using cosmetic composition B to confirm the skin brightness improvement effect of cosmetic composition B. The change in skin brightness was measured and shown in Table 7. The significance of the liver before and after the use of the cosmetic composition B was confirmed using the SPSS (Ver. 19) program (P <0.05), and the skin brightness improvement rate (%) compared to before use was calculated using the same calculation formula as in Example 3 ( 4).

Before use 2 weeks later 4 weeks later Skin Brightness (L) Average 63.01 63.13 63.55 ^* Deviation 2.46 2.36 2.45

^* P <0.05, number of subjects: 22

As a result, when using the composition of the present invention, it was found that after 4 weeks of use compared to before use, it showed a statistically significant skin brightness improvement rate of 0.86% (P <0.05, paired t-test).

Claims (13)

Cell growth factor;
Peptides;
Nucleotides;
vitamin;
glycerin;
Cellulose derivatives; And
A cell growth factor-containing composition having improved stability, including a hyaluronic acid salt. Cell growth factor;
Peptides;
Nucleotides;
vitamin;
glycerin;
Cellulose derivatives; And
A cell growth factor-containing composition, comprising hyaluronic acid salt, with improved skin absorption. The method according to claim 1 or 2,
The cell growth factor is,
Epidermal growth factor (EGF); And
A composition comprising fibroblast growth factor (FGF). The method according to claim 1 or 2,
The peptide,
At least one selected from the group consisting of acetylhexapeptide-8, cappertrippeptide-1, and palmitoylpentapeptide-4,
The nucleotide comprises adenosine,
The vitamin comprises niacinamide. The method according to claim 1 or 2,
The cellulose derivative,
A composition comprising at least one selected from the group consisting of ethyl cellulose, hydroxypropyl methyl cellulose, methyl cellulose, and hydroxyethyl cellulose. The method according to claim 1 or 2,
The hyaluronic acid salt, the composition containing sodium hyaluronate. The method according to claim 1 or 2,
The cell growth factor is,
A composition comprising human cell growth factor. The method according to claim 1 or 2,
The glycerin,
30 to 60% by weight, based on the total weight of the composition. The method according to claim 1 or 2,
The composition is based on the total weight of the composition,
The cell growth factor is 0.1 ppm or more and 20 ppm (w / w) or less,
The cellulose derivative in an amount of 0.05 wt% or more and less than 0.2 wt%,
At least 0.05 wt% and less than 0.2 wt% of the hyaluronic acid salt, the composition. The method according to claim 1 or 2,
The composition further comprises a peptide,
The peptides are contained in an amount of 0.0005 to 0.4% by weight based on the total weight of the composition. The method according to claim 1 or 2,
The composition,
Wrinkle improvement; For enhancing skin elasticity; For skin whitening; For pore reduction; For improving skin troubles; Or for moisturizing the skin. The method of claim 3,
The fibroblast growth factor comprises an acidic fibroblast growth factor or a basic fibroblast growth factor. The method of claim 11,
The composition comprises a cosmetic composition.

Images