JP2002293747A - Skin preparation for external use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin preparation for external use capable of sufficiently expressing effects such as improvement of a skin symptom caused by the material having a cell activating action, resistance to inflammation, therapy of injury, protection of aging of the skin, whitening and beautifying a darkened skin, improvement of rough skin and the like and excellent in stability, safety and feeling in application. SOLUTION: This skin preparation for external use is obtained by blending a plant-derived biologically active material in combination with a soluble shell membrane, a mucopolysaccharide and an amino acid.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin, and more particularly, to an effect excellent in improvement of skin symptoms, anti-inflammation, wound healing, prevention of skin aging, whitening of blackened skin, improvement of rough skin, etc. And a skin external preparation having excellent stability, safety and usability.

[0002]

2. Description of the Related Art In recent years, in the field of skin cosmetics and external preparations for skin, many studies have been made on external preparations for skin which activate skin function itself, improve skin symptoms and have anti-inflammatory effects. Among the skin constituent cells, fibroblasts in the dermis in particular are collagen fibers, elastic fibers, cells that produce a matrix, and by activating and activating these cells,
It is known that effects such as prevention of skin aging and improvement of rough skin can be obtained.

Conventionally, various substances such as allantoin, animal-derived proteins, ascorbic acid and its derivatives, plant extracts containing glycosides of vitamins and flavonoids have been used as such substances having a cell activating effect. It has been. Also, a skin external preparation and the like containing a soluble eggshell membrane as an active ingredient have been proposed.

[0004] However, the conventionally used components having the above-mentioned cell activating effect, or conventionally proposed external preparations for skin, etc., have insufficient action effects, poor stability and feeling of use. At present, a satisfactory skin external preparation has not yet been developed.

[0005]

Accordingly, the present invention is intended to improve the effects of a substance having a cell activating effect on skin symptoms, anti-inflammation, wound healing, prevention of skin aging, whitening of blackened skin, and improvement of skin roughness. An object of the present invention is to provide an external preparation for skin that can be sufficiently exerted, and that is excellent in stability, safety and usability.

[0006]

Means for Solving the Problems As a result of intensive studies to achieve the above object, the present inventors have found that a soluble eggshell membrane having a human dermal fibroblast cell growth-promoting action and a specific cell activating action. By using the substance in combination, the present inventors have found that synergistic effects such as improvement of skin symptoms, anti-inflammation, wound healing, prevention of skin aging, whitening of blackened skin, improvement of rough skin, etc. are obtained, and the present invention It was completed.

[0007] That is, the present invention provides a skin external preparation characterized by incorporating a soluble eggshell membrane, a mucopolysaccharide, an amino acid and a plant-derived physiologically active substance in order to achieve the above object.

[0008]

BEST MODE FOR CARRYING OUT THE INVENTION As the soluble eggshell membrane used in the present invention, a membrane (eggs membrane) adhering to the inside of the eggshell of bird eggs such as chicken eggs and quail eggs can be used as an acid agent, an alkali agent, an organic solvent,
Those generally known as those solubilized with an oxidizing / reducing agent, for example, those obtained by the methods described in JP-A-48-40943, JP-A-1-275512, etc. it can. The amount of the compound is 0.01 to 5% by weight, particularly preferably 0.1 to 3% by weight, based on the total weight of the external preparation for skin in consideration of the action of the soluble eggshell membrane on cells and the stability of the preparation. It is.
If the amount is less than 0.01% by weight, the above-mentioned various effects of the obtained external preparation for skin tend to decrease, while if it exceeds 5% by weight, the stability of the obtained external preparation for skin tends to decrease.

The mucopolysaccharide used in the present invention includes:
A long-chain polysaccharide composed of a repeating unit of a disaccharide composed of hexosamine (often N-acetylated glucosamine or galactosamine) and uronic acid (D-glucuronic acid or L-iduronic acid), or a salt or derivative thereof is used. Specific examples thereof include, but are not particularly limited to, hyaluronic acid, chondroitin sulfate, chondroitin, dermatan sulfate, heparan sulfate, heparin, keratan sulfate, and the like. One of these mucopolysaccharides may be used alone, or two or more of them may be used as necessary. The amount of the compound is preferably 0.001 to 10% by weight, particularly preferably 0.01 to 5%, based on the total weight of the external preparation for skin in consideration of the action of mucopolysaccharide on the skin and the stability of the preparation. % By weight. When the amount is less than 0.001% by weight, the above-mentioned various effects of the obtained external preparation for skin tend to decrease. On the other hand, when the amount exceeds 10% by weight, the stability of the external preparation for skin tends to decrease.

The amino acids used in the present invention include:
Promotes collagen and elastin production, or
It has an elastase activity inhibitory effect, and is not particularly limited, but includes glycine (Glycine), proline (Prolin)
e), Alanine, hydroxyproline (Hydr)
oxyproline), glutamic acid (Glutamic acid), arginine (Arginine), aspartic acid (Aspartic aci)
d), Serine, Lysine, Valine (V
aline), leucine (Leucine), threonine (Threonin)
e), Phenylalanine, Histidine, Cystine, Cysteic acid, Methionine, Isoleucine, Tyrosine, Hydroxylysine, Desmosin
e), amino acids such as lysinonorleucine, and the like. Oligopeptides, polypeptides, and proteins are not particularly limited, and soybean extract, silk extract, hydrolyzed silk solution, and the like. And furthermore, their derivatives and the like. One of these amino acids may be used alone, or two or more thereof may be used as necessary. In addition, considering the action of amino acids on cells and the stability of the preparation, the amount thereof is 0.000 to the total weight of the external preparation for skin.
001 to 10% by weight is preferable, and particularly preferably 0.0 to 10% by weight.
001 to 5% by weight. If the amount is less than 0.000001% by weight, the above-mentioned various effects of the obtained external preparation for skin tend to decrease, while if it exceeds 10% by weight, the stability of the obtained external preparation for skin tends to decrease.

As the plant-derived physiologically active substance used in the present invention, those exhibiting physiological activities such as whitening action (melanin production inhibitory action), anti-inflammatory action, anti-allergic action and antioxidant action are used. Examples include, but are not particularly limited to, lime extract, chamomile extract, gentian extract, licorice extract, button extract, assembly extract, chimpanzee extract, almond extract, carrot extract, pea extract, saxifrage Extract, mulberry extract and the like. These plant-derived physiologically active substances are
One type may be used as needed, or two or more types may be used. In addition, considering the action of the plant-derived physiologically active substance on the skin and the stability of the formulation,
It is preferably 0.01 to 10% by weight, particularly preferably 0.1 to 5% by weight, based on the total weight of the external preparation for skin. 0.
When the amount is less than 01% by weight, the above-mentioned various effects of the obtained external preparation for skin tend to decrease.
There is a tendency for the stability of the obtained external preparation for skin to decrease.

The external preparation for skin of the present invention may contain, in addition to the above-mentioned components, a humectant, an ultraviolet ray protective agent, a medicinal agent and the like which are usually added to the external preparation for skin. Examples of the humectant include polyethylene glycol, propylene glycol, glycerin, chitosan derivatives, saccharides, and water-soluble polymers. Examples of the ultraviolet ray protective agent include an ultraviolet ray absorbent such as a paraaminobenzoic acid derivative, a salicylic acid derivative, a cinnamic acid derivative and an urocanic acid derivative, and an ultraviolet ray scattering agent such as titanium oxide, zinc oxide and talc. Pharmaceutical agents include antimicrobial agents such as triclosan, biosol, benzalkonium chloride, benzethonium chloride, phenoxyethanol, and parabens for preventing infectious diseases caused by abnormal growth of foreign bacteria, nutritional supplementation, blood circulation promotion, and control of 5α-reductase. And a hair restorer component for the purpose.

The external preparation for skin of the present invention further comprises fats and oils, waxes, hydrocarbons, fatty acids, esters, surfactants, high molecular compounds, antioxidants, as long as the object of the present invention is not impaired. Agent, sequestering agent, dye, fragrance, pH
A regulator, a preservative, and the like can be appropriately blended.

The external preparation for skin of the present invention can be widely used as cosmetics, quasi-drugs or pharmaceuticals, and can be used in lotions such as lotions, serums, aqueous gels, emulsions, creams, etc. , Ointments, oils, and various other commonly used external preparations.

[0015]

The present invention will be described more specifically with reference to the following examples and comparative examples, but the present invention is not limited to the following examples.

Example 1, Comparative Examples 1 to 8 Lotions were prepared according to the formulation shown in Table 1 by a conventional method.

[0017]

[Table 1]

Each of the lotions of Example 1 and Comparative Examples 1 to 8 was applied to a female subject (25 to 25) complaining of spots, fine wrinkles, dry skin, etc.
For 30 subjects (55 years old), an appropriate amount was applied to the face twice a day (morning and evening) for one continuous month, and the skin condition before and after use was evaluated. The evaluation was performed by using each of the lotions of Example 1 and Comparative Examples 1 to 8 with a blind, and observing and evaluating the state of the skin before the start of the use test and after the end of the use test. The evaluation was performed in three stages of “I: improvement”, “II: slight improvement”, and “III: no change”, and the results are shown in Table 2.

[0019]

[Table 2]

Example 2, Comparative Examples 9-16 Emulsions were prepared according to the formulation shown in Table 3 by a conventional method.

[0021]

[Table 3]

Each of the emulsions of Example 2 and Comparative Examples 9 to 16 was applied to female subjects (25 to 25) who complained of spots, fine wrinkles, dry skin, etc.
For 30 subjects (55 years old), an appropriate amount was applied to the face twice a day (morning and evening) for one continuous month, and the skin condition before and after use was evaluated. The evaluation was carried out by blindly using each of the emulsions of Example 2 and Comparative Examples 9 to 16, and evaluated by observing the state of the skin before the start of the use test and after the end of the use test. The evaluation was performed in three stages: "I: improved", "II: slightly improved", and "III: no change". The results are shown in Table 4.

[0023]

[Table 4]

Example 3, Comparative Examples 17 to 24 Creams were prepared according to the formulation shown in Table 5 by a conventional method.

[0025]

[Table 5]

Each of the creams of Example 3 and Comparative Examples 17 to 24 was applied to a female subject (2) complaining of spots, wrinkles, dry skin, etc.
For 30 subjects (5 to 55 years old), an appropriate amount was applied to the face twice a day (morning and evening) for one continuous month, and the skin condition before and after use was evaluated. The evaluation was performed by using each of the creams of Example 3 and Comparative Examples 17 to 24 in a blind, and observing and evaluating the skin condition before the start of the use test and after the end of the use test. The evaluation was performed in three stages: "I: improved", "II: slightly improved", and "III: no change". The results are shown in Table 6.

[0027]

[Table 6]

As shown in Table 2, the soluble eggshell membranes, mucopolysaccharides, amino acids and various components of physiologically active substances derived from plants were independently blended. In comparison with Comparative Example 6 in which the compound was mixed, Comparative Example 7 in which a soluble eggshell membrane was mixed with a physiologically active substance derived from plants, Comparative Example 8 in which a soluble eggshell membrane was mixed with amino acids, or Comparative Example 1 in which no compound was mixed, In Example 1 of the present invention in which the components were combined and mixed, 77-87% of the subjects recognized that the skin condition was improved due to the synergistic effect of combining and mixing the various components.

As shown in Table 4, Comparative Examples 10 to 13 in which various components were blended alone, Comparative Example 14 in which a soluble eggshell membrane and mucopolysaccharide were blended, and a soluble eggshell membrane and a plant-derived physiologically active substance were blended Comparative Example 15, Comparative Example 16 in which a soluble eggshell membrane and an amino acid were blended, or Comparative Example 9 in which no amino acid was blended. In Example 2 of the present invention in which various components were combined and blended, various components were combined and blended. Due to the synergistic effect, 73-87% of the subjects recognized that their skin condition was improved.

As shown in Table 6, Comparative Examples 18 to 21 in which various components were blended alone, Comparative Example 22 in which soluble shell membrane and mucopolysaccharide were blended, and soluble shell membrane and plant-derived physiologically active substance were blended In Comparative Example 23, Comparative Example 24 in which a soluble eggshell membrane and an amino acid were blended, or Comparative Example 17 in which no amino acid was blended, in Example 3 of the present invention in which various components were combined and blended, various components were combined and blended. Due to the synergistic effect, 80-87% of the subjects recognized that their skin condition was improved.

During the period of use in the above evaluation test, in the group using the product of any of the examples,
None of the subjects complained of skin irritation such as pain with itching or erythema or skin symptoms such as allergic reaction. In addition, sedimentation, alteration, deterioration of the emulsified state, and the like of the components were not observed.

[0032]

EFFECT OF THE INVENTION The external preparation for skin of the present invention has an excellent human dermal fibroblast proliferation-promoting action due to a synergistic effect obtained by combining and mixing certain components such as a soluble eggshell membrane, and an excellent skin. It has the effects of improving symptoms, anti-inflammation, wound healing, preventing skin aging, whitening blackened skin, improving rough skin, etc., and is also excellent in stability, safety and usability.

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61K 31/198 A61K 31/198 4C206 31/726 31/726 35/54 35/54 38/00 A61P 17/16 A61P 17 / 16 A61K 37/02 F term (reference) 4C083 AA071 AA072 AA082 AA111 AA112 AC022 AC122 AC352 AC422 AC432 AC442 AC581 AC582 AD152 AD282 AD311 AD312 AD411 AD412 AD452 CC04 CC05 DD23 DD27 DD31 EE12 EE16 4C084 AA02MA03 MA03MA03 MA03 MA03MA03 ZA892 ZB111 ZB112 ZB131 ZB132 ZB211 ZB212 ZC201 ZC202 ZC211 ZC212 4C086 AA01 AA02 EA25 GA02 MA16 MA22 MA28 MA63 NA03 NA05 ZA89 ZB11 ZB13 ZB21 ZC21 4C087 AA01 AA02 BB61 CA03 MA47 MA17 MA03 Z0321 BA08 BA37 CA03 MA02 MA08 MA63 NA03 NA05 ZA89 ZB11 ZB13 ZB21 ZC21 4C206 AA01 AA02 FA53 MA36 MA42 MA48 MA83 NA03 NA05 ZA89 ZB11 ZB13 ZB21 ZC20 ZC21

Claims (5)

[Claims] 1. An external preparation for skin, comprising a soluble eggshell membrane, mucopolysaccharides, amino acids and a physiologically active substance derived from plants. 2. The mucopolysaccharide according to claim 1, wherein the mucopolysaccharide is one or more selected from the group consisting of a polysaccharide (glycosaminoglycan) comprising hexosamine and uronic acid, and salts or derivatives thereof. External preparation for skin. 3. An amino acid selected from the group consisting of amino acids, oligopeptides, polypeptides, proteins, and derivatives thereof, wherein the amino acids have a collagen or elastin production promoting action or an elastase activity inhibiting action. Or the external preparation for skin according to claim 1 or 2 which is two or more kinds. 4. A plant-derived physiologically active substance is selected from those exhibiting physiological activities such as whitening action (melanin production inhibitory action), anti-inflammatory action, anti-allergic action, antioxidant action and the like. The external preparation for skin according to any one of claims 1 to 3, which is: 5. The skin external preparation is a skin cosmetic.
5. The external preparation for skin according to any one of items 1 to 4.