KR20190066478A - Antimicrobial composition comprising the extracts of rambutan peel - Google Patents

Abstract

The present invention relates to an antimicrobial composition including the peel extract of rambutan (Nephelium lappaceum) fruits and, more specifically, to an antimicrobial composition with excellent antimicrobial activity by including the hexane fraction or ethyl acetate fraction of rambutan peel extract. The rambutan peel extract of the present invention shows excellent antimicrobial activity and accordingly, can be used in an antimicrobial pharmaceutical composition, health functional food, a cosmetic composition, an animal feed composition, food, additives for cosmetics or animal feed, various cleaning agents, and various detergents. Moreover, the rambutan peel extract of the present invention can be used beneficially in a pharmaceutical industry, a food industry, a livestock industry, a health industry, and a cosmetic industry by being easily processed into various forms such as liquid, cream, powder, ring, and pill since heat stability is excellent and the loss of activity does not appear in plasma and under an acid condition of pH 2.

Description

TECHNICAL FIELD [0001] The present invention relates to an antimicrobial composition containing an extract of Rambo peel as an active ingredient.

The present invention relates to rambutan ( Nephelium The present invention relates to an antimicrobial composition comprising a bark extract of lappaceum fruit, and more particularly, to an antimicrobial composition having an antibacterial activity including a hexane fraction or an ethyl acetate fraction of a lambutan bark extract.

Humans have used a variety of antimicrobials and preservatives to defend against the entry of pathogenic microorganisms and to control foodborne microorganisms. However, chemical antimicrobial agents and preservatives have excellent antimicrobial activity against various bacteria and fungi. However, when used for a long time, many side effects such as allergy-induced redness, vascular edema, myelosuppression, anemia, gastritis and degenerative brain diseases do. In the United States, the use of antibiotics, such as chloramphenicol, has been prohibited. Therefore, various studies on the development of natural antimicrobial agents using natural products with safety and long-term use have been underway.

According to the U.S. Center for Disease Control and Prevention, bloodstream infections in hospitals are one of the leading causes of death in the United States. Infections of pathogenic bacteria and fungi through skin and soft tissues are also recognized as universal medical diseases and are mainly caused by trauma or surgery. Many pathogenic bacterial and fungal infections are treated differently depending on the severity of the infection, but successful treatment is possible with oral antimicrobials and topical cleansing. However, the more severe or complex infections that are common in patients with underlying risk factors (e. G., Vascular flow disorders, diabetes) and / or infections caused by difficult or multi-resistant bacilli and fungi, Treatment with antimicrobial agents and removal of invasive surgical tissue may be required. For example, vancomycin, a glycopeptide antibiotic, has been used successfully for severe hospital infections caused by multi-drug-resistant Gram-positive bacteria, particularly MRSA, coagulase-negative staphylococcus, and Enterococcus. However, it has recently been reported that the isolation of strains resistant to vancomycin is increasing. As a current antibiotic, the treatment of severe infections caused by some bacteria in pathogens is very limited. Therefore, there is a growing need for more effective new antibiotic development for resistant bacteria.

On the other hand, as the population increases, fruit consumption is rapidly increasing, resulting in a large amount of fruit husks and seeds being generated as waste. Therefore, it is urgently necessary to study the reuse of spent shells and the like (Cheok CY et al., 2016, Critical Reviews in Food Science and Nutrition, http://dx.doi.org/10.1080/ 10408398.2016.1176009). Nephelium lappaceum is one of the most commonly consumed tropical fruits with many shells and belongs to the Sapindaceae family. It is 10 ~ 15m in height, with alternate leaves, 5 ~ 7 pairs of idolate leaves, thick It is glossy and has hairs on the back when it is young. The flowers bloom in April to June, the fruits are gathered in groups of 10 to 12, they are small eggshell size, ripened in July to August in red color, and have fleshy protuberances. The origin of rambutan is "rambut", originated in Malay and has the meaning of "head". Actual rambutan is one of the most common fruit of tropical fruit of Southeast Asia, and the largest producer is Thailand. Cultivated fruit is used to remove the peel and seeds and is usually used for reproductive purposes. It is also used for cooking in the form of jam or cooked as well as canned form for syrup.

In addition, Rambutan is a low-calorie fruit with a moisture content of 80%, carbohydrate of 17.5%, fat of 1.2%, protein of 1%, 79Kcal / 100g and contains a large amount of vitamin C, phosphorus, potassium, calcium and magnesium . Seed contains 38% crude lipid and oleic acid (42.0%) and arachidic acid (34.3%) are known to be the major components (ManaM et al., 2013. J. Oliec Sci. 62, 335-343). The rumen shell contains tannin and saponin, which are used as medicinal herbs. The main ingredient of the shell has recently been found to be geraniin (Elendran S et al., 2015, Pharm Biol. 53, 1719-1726). The geraniin accounts for 30% of the ethanol extract of the lamba shell and has anti-cancer and anti-diabetic activity and is known to be effective for prevention of hypertension (Elendran S et al., 2015, Pharm Biol. 53, 1719-1726). In addition, corilagin and ellagic acid have been reported from the shell (Thitilertdecha N et al., 2010. Molecules. 15, 1453-1465). In addition, the root of Rambutan is used as a cold treatment, the leaves are used as a poultice medicine, and the bark is used as a treatment for relieving muscles when a tongue is sick, but the antibacterial activity of the Rambutan peel extract is not known at all.

The physiological activities of humic substances such as antioxidant activity (Zhuang Y et al., 2017, Food Chem Toxicol. S0278-6915, 30030-30033), fatty acid synthesis inhibitory effect (Zhao YX et al., 2011, Carbohydr Res. 346, 1302-1306) and cancer cell growth inhibitory activity (Fang EF et al., 2015, Appl Biochem Biotechnol. 175, 3828-3839).

As a patent related to rambutane known so far, Korean Patent No. 10-1393007 [a cosmetic composition for preventing skin aging and improving skin wrinkles containing rambutane and rich extract as an active ingredient] is disclosed in Patent No. 10-1411947 Korean Patent Laid-Open No. 10-2006-0007083 discloses a method for producing a fruit wine using lambutan and a method for extracting a lambutan containing lambutan extract from Japanese Patent Laid-Open Publication No. 4-244004 Discloses a cosmetic composition containing a peel extract of lambutan and having a whitening effect. However, the antimicrobial activity of the antibacterial bark extract has not been known to date.

KR 10-1393007 B KR 10-1411947 B KR 10-2006-0007083 A JP 1992-244004 A

 Antioxidant and Antibacterial activities of Nephelium lappaceum L. extracts. LWT-Food Sci. Technol. 2008. 41, 2029-2035.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems occurring in the prior art, and it is an object of the present invention to provide an antibacterial composition containing a lambutan bark extract as an active ingredient.

In order to solve the above-mentioned problems, the present invention provides an antimicrobial composition containing a shell extract of Nephelium lappaceum as an active ingredient.

It is preferable that the above-mentioned lamba bark extract is an ethanol extract of a lamba shell.

Preferably, the above-mentioned lambutan skin extract is a hexane fraction or an ethyl acetate fraction of a lamellar shell ethanol extract.

The antimicrobial composition preferably has antimicrobial activity against Gram-positive bacteria.

The antimicrobial composition is preferably selected from the group consisting of a pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, a food additive, a cosmetic additive, an animal feed additive, a detergent and a cleanser.

INDUSTRIAL APPLICABILITY The antibacterial activity of the present invention exhibits excellent antimicrobial activity and can be used as an antibacterial pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, food, cosmetic or animal feed additives, various detergents, It is effective. In addition, the inventive Lambutan skin extract has excellent thermal stability and can be easily processed into various forms such as a liquid, a cream, a powder, a ring, and a tablet, since the acidic condition of pH 2 and the loss of activity do not occur even in plasma Pharmaceutical industry, food industry, animal husbandry industry, health industry and cosmetics industry.

Figure 1 shows the whole rambutan fruit,
Figure 2 shows the pulp region,
Fig. 3 shows the skin region,
Fig. 4 is a photograph showing the seed part. Fig.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition exhibiting a strong antimicrobial activity containing a lamba bark extract. The inventors of the present invention prepared fractions and water residues obtained by successively fractionating the ethanol extract of the lamba shell and an organic solvent of hexane, ethyl acetate and butanol in order to confirm a strong antimicrobial activity in the double hexene fraction and ethyl acetate fraction Respectively.

In particular, the active fractions exhibit a strong antibacterial activity against Gram-positive bacteria and can be developed as a broad-spectrum antibacterial agent against Gram-positive bacterial infections, thus completing the present invention.

Accordingly, the present invention relates to a process for the preparation of Nephelium lappaceum ) bark extract as an active ingredient.

It is preferable that the above-mentioned lamba bark extract is an ethanol extract of a lamba shell.

Preferably, the above-mentioned lambutan skin extract is a hexane fraction or an ethyl acetate fraction of a lamellar shell ethanol extract.

The antimicrobial composition preferably has antimicrobial activity against Gram-positive bacteria.

The antimicrobial composition is preferably selected from the group consisting of a pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, a food additive, a cosmetic additive, an animal feed additive, a detergent and a cleanser.

In a preferred embodiment, the present invention relates to a method for the production of a tambourine, comprising the steps of separating the shell, pulp, seed from the selected line, washed rambutan fruit; Preparing an extract from the separated shell; Purifying an active fraction through a sequential organic solvent fraction of a lambutan bark extract; And evaluating the stability of the active fraction.

The "lambutan bark extract" contained in the composition of the present invention comprises a step of recovering a shell from imported lambutan fruit, a step of extracting the shell with a solvent, a step of filtering the extract using a filter net of 0.06 mm or less, ≪ / RTI >

In the present invention, "lambutan skin extract" can be prepared using various extraction solvents. For example, water (cold water, hot water), alcohol, water, or a lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, alcohol, propanol, butanol, etc.) can do. Preferably extracts using ethanol or alcohol, shows the maximum yield of active material.

Further, the "lambutan skin extract" of the present invention may be present as an active fraction by an additional organic solvent fraction after the extraction process using the above extraction solvent. As a preferred embodiment, the inventive lamellar shell ethanol extract can be obtained as a hexane fraction, an ethyl acetate fraction, a butanol fraction and a water residue after an organic solvent fraction by performing sequential organic solvent fractions of hexene, ethyl acetate and butanol.

The inventive Lambutan bark extract and its organic solvent fraction can be made into powders through conventional powdering processes such as vacuum distillation, freeze-drying, spray drying and the like. They maintain their activity even at 100 ° C heat treatment and pH 2 in human body.

The inventive lamba bark extract and active fractions thereof exhibit a strong antibacterial activity and are useful as pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, foods, cosmetics, animal food additives, and pharmaceutical compositions relating to the prevention or treatment of pathogenic bacteria It can be used as a material for various cleaning agents.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a pharmaceutical composition.

The pharmaceutical composition may be formulated into various forms such as powders, granules, tablets, capsules, oral formulations such as suspensions, emulsions, syrups and aerosols, injections of sterilized injection solutions, etc. And can be administered via various routes including oral administration or intravenous, intraperitoneal, subcutaneous, rectal, topical administration, and the like. Such pharmaceutical compositions may further comprise carriers, excipients or diluents, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, But are not limited to, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, amorphous cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, And the like. In addition, the pharmaceutical composition of the present invention may further include a filler, an anti-coagulant, a lubricant, a wetting agent, a flavoring agent, an emulsifying agent, an antiseptic, and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will depend on the type of disease, severity, The sensitivity to the drug, the time of administration, the route of administration and the rate of release, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiply. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without side effects, which can be easily determined by those skilled in the art.

The effective amount of the component having antimicrobial activity in the pharmaceutical composition of the present invention may vary depending on the age, sex and body weight of the patient, and is generally from 1 to 5,000 mg, preferably from 100 to 3,000 mg per body weight, Or one to three times a day. However, the dosage may not be limited in any way because it may be increased or decreased depending on route of administration, severity of disease, sex, weight, age, and the like. The pharmaceutical composition of the present invention can be administered to a subject through various routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine or intra-cerebroventricular injections. In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the administration route of the pharmaceutical composition of the present invention is either oral or non-oral May be administered orally. The composition of the present invention may also be administered using any device capable of delivering an effective ingredient to a target cell. In the present invention, the term "object" includes, but is not limited to, human, monkey, cow, horse, sheep, pig, chicken, turkey, quail, cat, dog, mouse, rat, rabbit or guinea pig , Preferably a mammal, more preferably a human.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a health functional food.

The health functional food of the present invention can be used variously for effective foods and beverages which can lower the possibility of infection from a subject having a high risk of infection by a pathogenic microorganism. Examples of food containing the active ingredient having excellent antimicrobial activity of the present invention include various foods, beverages, gums, tea, vitamin complexes, health supplement foods and the like, and may be in the form of powders, granules, tablets, capsules or beverages . The active ingredient of the present invention may generally be added in an amount of 0.01 to 15% by weight of the total food, and the health beverage composition may be added in a proportion of 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml. The health functional food of the present invention may contain, as an additional ingredient, a food-acceptable food-aid additive such as natural carbohydrates and various flavors, in addition to containing the above-mentioned compound as an essential ingredient in the indicated ratio. Examples of the natural carbohydrate include sugar sugars such as glucose, monosaccharides such as fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of the flavor agent include tau martin, rebaudioside A, glycyrrhizin, saccharin, and aspartame. The proportion of the above-mentioned flavoring agent is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention may contain various kinds of nutrients, vitamins, minerals, flavors such as synthetic flavors and natural flavors, colorants and heavy stabilizers, pectic acid and its salts, alginic acid and its salts, Thickening agents, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. In addition, the health functional food of the present invention may contain flesh for producing natural fruit juice, fruit juice drink, vegetable drink and the like. These components may be used independently or in combination. The ratio of such additives is generally selected in the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a cosmetic composition.

The cosmetic composition of the present invention may be manufactured in the form of an ingredient having excellent antimicrobial activity of the present invention in the form of an essence, a nutritional cream, a body lotion, a rinse, a shampoo, a body cleanser, a lotion, a skin, But is not limited thereto. The preparation in this form may be easily carried out according to various production processes known to those skilled in the art.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an animal feed composition.

The animal is an organism corresponding to a plant and mainly consumes organic matter as nutrients and refers to the digestion, reproduction and differentiation of the respiratory organs. Specifically, the animal is an animal such as an echinoderm, crustacean, mollusk, fish, amphibian, reptile, Mammals and preferably molluscs such as echinoderms such as echinoderms or sea cucumbers, arthropods including crustaceans such as crabs, shrimps and prawns, cephalopods, gastropods or bivalves such as sea buckets, sea breams, codfish, , Flounder or the like, poultry such as pheasant or chicken, or a mammal such as pig, cattle, or goat.

The composition for animal feed can further comprise cereals, vegetable protein feeds, animal protein feeds, sugar or dairy products in the antimicrobial composition comprising the antimicrobial component of the present invention. The cereal may be specifically wheat, crushed or crushed wheat, oats, barley, corn and rice, and the vegetable protein feed may be one mainly composed of rapeseed, bean and sunflower, and the animal protein feed is specifically May be blood, meat, bone meal, and fish meal, and the sugar or dairy product may specifically be a dry ingredient consisting of various powdered milk and whey powders.

The animal feed composition may further contain ingredients such as nutritional supplements, digestion and absorption enhancers, sex-promoting agents or disease prevention agents.

The antimicrobial composition contained in the composition for animal feed of the present invention may be varied depending on the intended use and the conditions of use of the feed. For example, 0.1 to 100 g of the antimicrobial composition may be contained based on 1 kg of the final feed.

In addition, the feed composition can be made into a light-viscous granulated or granulated material depending on the degree of comminution of the components, which can be fed into a mesh or formed into a desired discrete shape for further processing and packaging, Pelletizing, expanding or extruding processes, and preferably excess water can be dried off for ease of storage.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an additive for foods, cosmetics or animal feeds.

The additive is added to impart preservability to food, cosmetics or animal feed used. Since the antimicrobial composition is a natural ingredient and is not harmful to human body, it can be safely applied to foods, cosmetics or animal feeds.

The additive may further include other optional additives selected depending on the purpose and use of the additive, and may be mixed with other active ingredients, such as commonly used additives, or additives such as pigments, surfactants, and preservatives . The additive may be prepared by pulverizing, granulating, tableting or liquefying according to the purpose and use of the additive. For commercialization, a conventional method may be used for packaging.

The amount of the antimicrobial composition to be used may be appropriately used depending on the purpose of use, the application form, the application site, the object to be applied, and the like. For example, the antimicrobial composition may contain 0.01 to 50 parts by weight, The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

In a preferred embodiment, the present invention can be applied as an application of a cleaning agent.

The detergent may preferably be a detergent for clothes, a dishwasher, a food cleaner, or a household appliance cleaner. The antimicrobial composition is a natural ingredient and is not harmful to human body. Therefore, it is more preferably used for the purpose of washing or antibacterial property of various daily necessities including kitchen utensil or food washing.

The detergent may further contain an adversely selected additive depending on the purpose and use of the detergent. The detergent may be mixed with other active ingredients, such as detergents, and additives such as pigments, surfactants and preservatives. The detergent may be prepared by pulverizing, granulating, tableting or liquefying according to the purpose and use of the detergent, and a conventional method may be used for packaging for commercialization.

The amount of the antimicrobial composition to be used in the cleaning agent may be appropriately used depending on the purpose of use, the application form, the application site, the article to be applied, and the like. For example, the antimicrobial composition may contain 0.01 to 50 parts by weight, The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural ingredient and is not harmful to human body, it can be used in various amounts as long as its application purpose can be achieved.

As a preferred embodiment, the present invention can be applied as an antibacterial composition for use as a cleansing agent.

The cleaner can be used for hand, oral cavity or ladies' cleanser in a simple manner to prevent infection of a pathogenic microorganism which is easily infected in daily life. Since the antimicrobial composition is a natural ingredient and is not harmful to the human body, it can be safely applied even when it is used as a hand, mouth, and female cleanser.

The cleansing agent may further include other additives optionally selected depending on the purpose and use of the cleansing agent. The cleansing agent may be mixed with other active ingredients, such as commonly used additives, and additives such as coloring matters, surfactants and preservatives . The cleansing agent may be prepared by pulverization, granulation, tableting or liquefying according to the purpose and use of the cleansing agent.

In addition, the amount of the antimicrobial composition to be used in the cleaner may be suitably used according to the purpose of use, application form, application site, and the like. For example, the amount of the antimicrobial composition may be 0.01 to 50 parts by weight, But is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

Hereinafter, the present invention will be described in more detail by way of examples. The following examples are only exemplary embodiments of the present invention, and the scope of the present invention is not limited to the scope of the following examples.

[Example]

Example  One: Lambutan  Preparation of extracts by site

After purchasing the fruits of Ramabutan (moisture content 80%) from Thailand, the foreign materials were removed and the shells, flesh and seeds were respectively classified (Fig. 1) and used for producing ethanol extracts. Ethanol extracts were prepared by adding 10 times ethanol (95%, Duksan, Korea) to each sample and once extracting at room temperature. The extracts were collected by filtration and concentrated under reduced pressure to prepare powders. Total polyphenols, total flavonoids, total sugars and reducing sugar content were determined by analysis of the constituents of the prepared extracts. Total polyphenol content was determined by adding 50 μl of Folin-ciocalteau and 100 μl of saturated Na ₂ CO ₃ solution to 400 μl of the extract solution, leaving it at room temperature for 1 hour and measuring the absorbance at 725 nm. Tannic acid was used as a standard reagent. The total flavonoid content of each sample was measured by stirring for 18 hours in methanol. To the 400 μl of the filtered extract, 4 ml of 90% diethylene glycol was added and 40 μl of 1N NaOH was added. The reaction was carried out at 37 ° C. for 1 hour and then absorbance was measured at 420 nm. As a standard reagent, rutin was used. Reducing sugar was quantified by DNS method and total sugar was quantified by phenol-sulfuric acid method.

[Table 1] Lambutan  Yield and composition of extracts by site

As a result, as shown in Table 1, the extraction yields of the parts of the rambutan fruits were in the order of shell, pulp and seed, and showed a high yield of 7.33% in the shell. Analysis of total polyphenol contents showed a very high total polyphenol content (284.6 mg / g) in the peel extract and relatively low total flavonoid content. On the other hand, the total sugar content and the reducing sugar content of the seeds were low, but the total flavonoids were 12.6 mg / g total polyphenol and 4.6 mg / g.

Example  2: Lambutan  Evaluation of Antimicrobial Activity of Extracts by Site

The antimicrobial activity of the ethanol extract of various parts of the rambutane obtained in Example 1 was evaluated, and the results are shown in Table 2. As the strains used for the evaluation of antimicrobial activity, Escherichia coli KCTC 1682, Proteus vulgaris KCTC 2433, Pseudomonas aeruginosa KACC 10186 , was used in the Salmonella typhimurium KCTC 1926, Gram-positive bacteria are Staphylococcus aureus KCTC 1916, Staphylococcus epidermidis ATCC 12228, Listeria monocytogenes KACC 10550 and Bacillus subtilis KCTC 1924 were used. To evaluate the antifungal activity, Saccharomyces cerevisiae IF0 0233 and Candida albicans KCTC 1940 was used.

First, the case wherein the bacterial activity evaluation, Nutrient broth (Difco Co., USA) and then inoculated to each of the bacteria were cultured at 37 ℃ for 24 hours, by adjusting the respective strain to OD ₆₀₀ 0.1 Nutrient agar (Difco Co. (Diameter: 6.5 mm, Whatsman No. 2) was added to each well of a sterilized petri dish (90 mm, Green Cross, Korea) containing 10% . In the case of fungi, Sabouraud dextrose (Difco Co., USA) was cultured in the same manner at 30 ° C. for 24 hours, and the size of the growth inhibition rings was measured to evaluate the antimicrobial activity. As a control, ampicillin, an antimicrobial agent, and miconazole (Sigma Co., USA), an antifungal agent, were used at a concentration of 1 μg / disc, respectively. The size of the growth inhibition ring was determined by the naked eye mm, and was expressed as representative results after three or more evaluations.

[Table 2] Lambutan  Antibacterial activity of extracts

As a result, as shown in Table 2, in the case of ampicillin (1 μg / disc) used as an anti-bacterial active control, all bacteria except for E. coli and P. aeruginosa were grown at a growth of 20.0-30.0 mm (1μg / disc) of antifungal agent showed 15.5mm and 18.0mm growth inhibition against C. albicans and S. cerevisiae , respectively. On the other hand, in the case of rambutan skin extract exhibited a growth inhibition ring of 14.0mm for Staphylococcus epidermidis and Staphylococcus aureus at 500μg / disc density, Proteus and 13.5mm growth inhibition ring against the vulgaris . On the other hand, the antibacterial activity of the rambutan pulp and seed extract was not exhibited at all. In addition, no antifungal activity was observed for the used fungi in all of the rambutane samples. These results suggest that the lamba shell extract can be effectively applied to the control of some Gram - positive bacteria.

Example 3: Preparation of sequential organic solvent fractions from a lambutan bark extract and analysis of useful components thereof

On the basis of the above results, a large amount of the ethanol extract of the lamba shell was prepared. The extract was fractionated with hexane, ethyl acetate and butanol sequentially, and finally the water residue was recovered. The organic solvent fraction efficiency and component analysis results are shown in Table 3. The total polyphenols, total flavonoids, total sugars and reducing sugar content were measured in the same manner as in Example 1.

[Table 3] Lambutan  Peel ethanol extract and its sequential Fraction  Yield and component analysis

As shown in Table 3, the butanol fraction and the ethyl acetate fraction of the lambutan bark extract fraction had the highest fractionation efficiencies of 63.8% and 28.5% and 5.3%, respectively. On the other hand, the hexane fractions accounted for 1% of the extract, indicating that the oil - soluble oil component of the lambutan skin extract was in a very low state. Thus, 0.073 g of the hexane fraction and 2.08 g of the ethyl acetate fraction were recovered from 100 g of the lamba shell.

The total polyphenol contents of the fractions were in the order of ethyl acetate fraction, butanol fraction, water residue and hexene fraction. Total flavonoid content was also highest in the ethyl acetate fraction, and the butanol fraction and water residue were similar in the range of 8.5 to 9.9 mg / g. On the other hand, total sugar and reducing sugar contents were the highest in butanol fraction, followed by water residue. Therefore, the hexane fraction and the ethyl acetate fraction of the lambutan bark extract were expected to exhibit various physiological activities and expected to exhibit a strong antioxidant activity.

Example  4: Lambutan  Sequential of bark extract Organic solvent Fraction  Evaluation of antimicrobial activity

The antibacterial activities of the organic solvent fractions of the antibacterial ethanol extract prepared in Example 3 were evaluated. The results are shown in Table 4.

[Table 4] Lambutan  Sequence of ethanol extract of skin Organic solvent Fraction  Antimicrobial activity

As shown in Table 4, ampicillin (1 μg / disc) used as an anti-bacterial activity control showed strong antibacterial activity against bacteria except for E. coli and P. aeruginosa . Myconezol (1 μg / disc), an antifungal agent, also exhibited excellent antifungal activity against the fungi used. Among the fractions of ethanol extract of rambutan shells have showed excellent antibacterial activity only hexene fraction ethyl acetate fraction, in particular, unlike the bark ethanol extract of rambutan, Listeria monocytogens and Bacillus subtilis showed strong antibacterial activity. The above results suggest that the lambutan bark extract and its hexane fraction and ethylacetate fraction can be effectively used to control foodborne pathogens and pathogenic bacteria.

Example  5: Lambutan  Bark extract and its Hexen Fraction  Ethyl acetate Fraction  Acid and thermal stability evaluation

The antibacterial activity against the antibacterial activity and the acid stability of the antibacterial activity of the antibacterial extracts obtained in Example 1 and Example 3 and the hexane fraction and ethyl acetate fraction thereof were confirmed. The samples exhibited high thermal and acid stability because they did not show a significant decrease in antimicrobial activity upon heat treatment at 100 ° C for 1 hour and pH 2 (0.01M HCl) for 1 hour. Therefore, it is expected that the above-mentioned antibacterial bark extract and active fractions thereof will maintain excellent antibacterial activity under various processing steps.

Claims (5)

Nephelium lappaceum ) bark extract as an active ingredient. The antimicrobial composition according to claim 1, wherein the antibacterial shell extract is an ethanol extract of a lamba shell. The antimicrobial composition according to claim 1, wherein the antibacterial shell extract is a hexane fraction or an ethyl acetate fraction of a lamellar shell ethanol extract. 4. The antimicrobial composition according to any one of claims 1 to 3, wherein the antimicrobial composition has an antimicrobial activity against Gram-positive bacteria. 4. The composition according to any one of claims 1 to 3, wherein the antimicrobial composition is selected from the group consisting of a pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, a food additive, a cosmetic additive, an animal feed additive, . ≪ / RTI >

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