KR102287389B1 - Antimicrobial composition comprising the extract of flower bud of panax ginseng c. a. meyer - Google Patents

Abstract

The present invention relates to an antibacterial composition containing an extract of a flower bud of Panax ginseng CA Meyer as an active ingredient, and more particularly, to a broad-spectrum antibacterial composition containing an ethanol extract of a ginseng bud as an active ingredient. will be. The ginseng bud extract as an active ingredient of the broad-spectrum antibacterial composition of the present invention, as demonstrated through the examples of the present specification, is an antibacterial pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, food, cosmetic or animal feed. It has an excellent effect that it can be used as an additive, various detergents, and various cleaning agents. In addition, the ginseng bud extract of the present invention has excellent thermal stability and no loss of activity even under acidic conditions of pH 2 and plasma, so it can be easily processed into various forms such as liquid, cream, powder, pill, tablet, etc. Therefore, it can be very usefully used in the pharmaceutical industry, food industry, livestock industry, health industry and cosmetics industry.

Description

Antimicrobial composition containing ginseng bud extract as an active ingredient {ANTIMICROBIAL COMPOSITION COMPRISING THE EXTRACT OF FLOWER BUD OF PANAX GINSENG C. A. MEYER}

The present invention relates to an antibacterial composition containing an extract of a flower bud of Panax ginseng CA Meyer as an active ingredient, and more particularly, to a broad-spectrum antibacterial composition containing an ethanol extract of a ginseng bud as an active ingredient. will be.

Humans have used various antibacterial agents and preservatives to defend against the invasion of pathogenic microorganisms and to control food spoilage microorganisms. However, in the case of chemical antibacterial agents and antiseptics, they show excellent antibacterial activity against various bacteria and fungi, but when used for a long time, many side effects such as redness due to allergy, angioedema, bone marrow suppression, anemia, gastritis, and degenerative brain disease appear. do. In fact, in the United States, the use of antibiotics with severe side effects, such as chloramphenicol, has been banned. Therefore, various studies on the development of natural antibacterial agents using natural products that have been used for a long time while ensuring safety are in progress.

Meanwhile, according to the U.S. Center for Disease Control and Prevention, in-hospital bloodstream infection is one of the leading causes of death in the United States. In addition, infection of pathogenic bacteria and fungi through the skin and soft tissues is recognized as a universal medical disease, and it is understood that it is mainly caused by trauma or surgery. Many pathogenic bacterial and fungal infections have different treatment methods depending on the severity of the infection, but successful treatment is possible with oral antimicrobial agents and topical cleaning. However, more severe or complex infections that commonly occur in patients with underlying risk factors (eg, vascular blood flow abnormalities, diabetes) and/or infections caused by difficult-to-treat or multi-resistant bacteria and fungi include intravenous Measures such as antimicrobial treatment and invasive surgical removal may be necessary. For example, the glycopeptide antibiotic vancomycin has been used successfully against severe nosocomial infections caused by multi-drug-resistant Gram-positive bacteria, particularly MRSA, coagulase-negative Staphylococcus and Enterococcus. However, it has been recently reported that the isolation of strains resistant to vancomycin is increasing, and it is true that current antibiotics are very limited in treating severe infections caused by some of the pathogens. Therefore, the need for the development of new more effective antibiotics against increasingly resistant bacteria is increasing.

On the other hand, ginseng ( Panax ginseng CA Meyer) is a perennial herb belonging to the genus Panax of the Araliaceae family. There are vertical wrinkles and fine root marks. At the root, there is an outcrop to which the residues of the stem are attached. In oriental medicine, the root of ginseng is mainly used as a medicinal material, and it is said to be used for body weakness, malaise, fatigue, anorexia, vomiting, and diarrhea. In fact, known pharmacological actions include cerebral cortex excitation and inhibition, balance, anti-fatigue, anti-aging, immune enhancement, heart contraction, gonad stimulation, hyperglycemia suppression, protein synthesis promotion, homeostasis maintenance, anticancer, detoxification, etc. It has an odour, the taste is sweet and slightly bitter, and the nature is slightly warm. In addition to the root, ginseng leaves, stems, fruits and seeds are all edible. In particular, the leaves of ginseng are used as ginseng leaves, the outdus are used as ginseng, and the seeds are used as ginseng. It is also called ja (人蔘子), and all of them are used as precious medicinals in oriental medicine.

Although research on the underground part of ginseng is intensively conducted, research on ginseng flower buds is very insignificant. In Korea, 1 peduncle is produced in ginseng in summer, and 4 to 40 light green small flowers hang on the umbel at the end, and flowers usually start to bloom from 3 to 4 years old and bloom in mid-May. Ginseng flowers are discarded without a separate use, and are more economical than ginseng roots, more stable in terms of supply, and economically advantageous. In addition, it is known that saponin content is higher in ginseng leaves and ginseng flowers than in ginseng root. So far, studies related to ginseng flowers and flower buds (flowers that have not yet bloomed, just before the ginseng flower blooms, the flower buds have developed considerably and are close to flowering), include analysis of the fragrance component of ginseng flowers (Chang-Sik Woo, 2009. Ajou University). The effect of oral administration of fermented ginseng flower extract on the production of mouse cytokines IL-6 and TNF-α (Jeong Jung et al.) , 2014. Journal of the Korean Society for Food and Nutrition 27: 43-49) and antioxidant activity of ginseng flowers according to fermenting microorganisms (Kyung-Hee Kim et al., 2013. Journal of Food and Nutrition Sciences 42: 663-669), most of which are antioxidants of ginseng flowers. report on activity.

As patents related to ginseng buds, Korean Patent Registration No. 10-1707522 [composition for external application for skin containing ginseng flower extract] and [ginseng flower powder and manufacturing method thereof, and cosmetic composition containing the same] in No. 10-1784992 ], [Method for producing complex extract of ginseng flower with enhanced active substances, and functional cosmetic composition prepared therefrom] in No. 10-1656710, [Composition for animal feed using ginseng flowers] in No. 10-1944039, No. 10 [Shampoo and rinse composition added by stabilizing ground extract of ginseng] in No. -0017300, [Composition for external application containing ginseng flower essential oil] in No. 10-1601022, [Scent of ginseng flower] in No. 10-1438368 Most of the registered patents related to cosmetics, such as a perfume composition that reproduces [Composition for animal feed using ginseng flower], No. 10-0894029 [Pharmaceutical composition for the prevention and treatment of diabetes using ginseng flower as a raw material], No. 10-1016996 [Ginsenoside A composition for the prevention and treatment of cardiovascular diseases containing a composition for the prevention and treatment of cardiovascular diseases] and [Method for producing ginseng bud tea] are also disclosed in No. 10-0004149. In addition, in Korean Patent Laid-Open No. 10-2018-0088226 [Composition containing ginseng flower extract with enhanced ginsenoside content as an active ingredient] and [Ginseng flower with irritation relief and whitening ability] in No. 10-2013-0050568 Method for producing a fermented product] and the like are disclosed. However, there is no known ginseng bud extract that exhibits antibacterial activity against various Gram-positive bacteria and Gram-negative bacteria related to allergic food poisoning, such as Proteus vulgaris.

US 10-2018-0088226 A

Heejin Do, 2013, Seokyeong University Master's thesis, Skin improvement effect of ginseng extracts for each part

The present invention has been devised to solve the problems of the prior art as described above, and an object to be solved in the present invention is to provide a broad-spectrum antibacterial composition containing a ginseng bud extract as an active ingredient.

In order to solve the above problems, the present invention provides an antibacterial composition containing an extract of ginseng flower buds (Flower bud of Panax ginseng CA Meyer) as an active ingredient.

The ginseng bud extract is preferably an ethanol extract of the ginseng bud.

The antimicrobial composition preferably has antimicrobial activity against Gram-positive bacteria.

The antibacterial composition preferably has antibacterial activity against Proteus vulgaris.

The antimicrobial composition is preferably selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food additives, cosmetic additives, animal feed additives, detergents and cleaners.

The ginseng bud extract as an active ingredient of the broad-spectrum antibacterial composition of the present invention, as demonstrated through the examples of the present specification, is an antibacterial pharmaceutical composition, a health functional food, a cosmetic composition, an animal feed composition, food, cosmetic or animal feed It has an excellent effect that it can be used as an additive, various cleaning agents, and various cleaning agents. In addition, the ginseng bud extract of the present invention has excellent thermal stability and no loss of activity even under acidic conditions of pH 2 and plasma, so it can be easily processed into various forms such as liquid, cream, powder, pill, tablet, etc. Therefore, it can be very usefully used in the pharmaceutical industry, food industry, livestock industry, health industry and cosmetics industry.

1 shows ginseng roots, ginseng leaves, ginseng buds and ginseng seeds used in Examples of the present invention: 1: ginseng root, 2: ginseng leaf, 3: ginseng bud, 4: ginseng seed

Hereinafter, the present invention will be described in detail.

The inventors of the present invention prepared a ginseng bud extract by a certain method in order to test the antibacterial efficacy of ginseng buds, and recovered it as an antibacterial active ingredient, and the extract has excellent thermal stability and acid stability. By confirming the ginseng bud extract was intended to be used as an antibacterial composition.

In particular, the active extract showed a strong antibacterial activity against Proteus vulgaris among Gram-positive bacteria and Gram-negative bacteria, and confirmed that it can be developed as a broad-spectrum antibacterial agent for various bacterial infectious diseases, thereby completing the present invention.

Specifically, the present inventors prepared an ethanol extract for ginseng buds in order to develop an antibacterial composition using ginseng buds known to have anticancer, anti-inflammatory, hepatoprotective, and antihypertensive effects, and various bacteria and fungi thereof the evaluation of the antibacterial activity on, exhibits a strong antibacterial activity against the ginseng and a strong antibacterial activity against the bud extract of gram-positive bacteria, Gram-negative bacteria of the known food poisoning bacteria Proteus vulgaris (Proteus vulgaris), wherein the said extract broad It was confirmed that it can be used as a bacterial agent.

Accordingly, the present invention provides an antibacterial composition containing a ginseng bud extract as an active ingredient.

The ginseng bud extract is preferably extracted with a solvent selected from the group consisting of water, alcohol and anhydrous or hydrous alcohol having 1 to 4 carbon atoms, and ethanol extraction is most preferred.

The ginseng bud extract may be fractionated sequentially or separately with an organic solvent of hexene, ethyl acetate and butanol to additionally obtain a hexene fraction, an ethyl acetate fraction, a butanol fraction, and a water residue.

In addition, the antimicrobial composition is preferably selected from the group consisting of pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food additives, cosmetic additives, additives for animal feed, detergents and cleaners.

Hereinafter, the manufacturing method and efficacy experiments of the ginseng bud extract of the present invention will be described in more detail.

The inventors of the present invention in order to achieve the object of the present invention, preparing a ginseng bud extract; Evaluating the antibacterial activity of the extract; and stability investigation of the ginseng bud extract.

The "ginseng bud extract" contained in the composition of the present invention includes the steps of extracting the buds of 3- to 4-year-old ginseng with an organic solvent and filtering the extract using a filtration net of 0.06 mm or less, and concentrating it under reduced pressure. can be obtained by

The solvent used in the present invention is water (cold water, hot water), alcohol, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, alcohol, propanol, butanol, etc.), a mixed solvent of the lower alcohol and water, etc. hot water, or 95% ethanol extraction is most preferred.

As a preferred embodiment, the ginseng bud of the present invention may be extracted with hot water or ethanol. In addition, the hot water or ethanol extract may be fractionated sequentially or separately with an organic solvent of hexene, ethyl acetate and butanol to additionally obtain a hexene fraction, an ethyl acetate fraction, a butanol fraction and a water residue.

The ginseng bud extract of the present invention may be prepared as a powder through a conventional powdering process such as drying under reduced pressure, freeze drying, or spray drying. Ginseng bud extract is not degraded by various degrading enzymes in plasma, and it maintains antibacterial activity even at 100℃ heat treatment and pH 2 in the human stomach.

The ginseng bud extract of the present invention exhibits strong antibacterial activity, and is used in pharmaceutical compositions, health functional foods, cosmetic compositions, animal feed compositions, food, cosmetics, additives for animal feed and various cleaners related to the prevention or treatment of pathogenic bacterial infections. material can be used.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a pharmaceutical composition.

The pharmaceutical composition is formulated in various forms, such as oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, and injections of sterile injection solutions, according to conventional methods for each purpose of use. It can be used, and it can be administered through various routes including oral administration or intravenous, intraperitoneal, subcutaneous, rectal, topical administration, and the like. The pharmaceutical composition may further include a carrier, excipient, or diluent, and examples of suitable carriers, excipients or diluents that may be included include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, Starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methyl cellulose, amorphous cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil and the like. In addition, the pharmaceutical composition of the present invention may further include a filler, an anti-agglomeration agent, a lubricant, a wetting agent, a flavoring agent, an emulsifier, a preservative, and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type, severity, activity of the drug, and the type of disease in the patient; Sensitivity to the drug, time of administration, route of administration and rate of excretion, duration of treatment, factors including concomitant drugs, and other factors well known in the medical field. The pharmaceutical composition of the present invention may be administered as an individual therapeutic agent or may be administered in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiple times. In consideration of all of the above factors, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, which can be easily determined by those skilled in the art.

The effective amount of the component having antibacterial activity in the pharmaceutical composition of the present invention may vary depending on the age, sex, and weight of the patient, and generally 1 to 5,000 mg per body weight, preferably 100 to 3,000 mg per body weight, is administered daily or every other day Or it can be administered in divided doses 1 to 3 times a day. However, since it may increase or decrease depending on the route of administration, disease severity, sex, weight, age, etc., the dosage is not intended to limit the scope of the present invention in any way. The pharmaceutical composition of the present invention may be administered to a subject through various routes. Any mode of administration can be envisaged, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intra-cerebroventricular injection. In the present invention, "administration" means providing a predetermined substance to a patient by any suitable method, and the administration route of the pharmaceutical composition of the present invention is oral or parenteral through all general routes as long as it can reach the target tissue. It can be administered orally. In addition, the composition of the present invention may be administered using any device capable of delivering an active ingredient to a target cell. In the present invention, "subject" is not particularly limited, but includes, for example, humans, monkeys, cattle, horses, sheep, pigs, chickens, turkeys, quails, cats, dogs, mice, rats, rabbits or guinea pigs. and preferably a mammal, more preferably a human.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a dietary supplement.

The health functional food of the present invention can be used in various ways, such as effective foods and beverages that can lower the possibility of infection from a subject with a high risk of infection by pathogenic microorganisms. Foods containing the active ingredient having excellent antibacterial activity of the present invention include, for example, various foods, beverages, gum, tea, vitamin complexes, health supplements, and the like, in powder, granule, tablet, capsule or beverage form. can be used as In general, the active ingredient of the present invention may be added in 0.01 to 15% by weight of the total food weight, and the health beverage composition may be added in a proportion of 0.02 to 10 g, preferably 0.3 to 1 g, based on 100 ml. In addition to containing the above compound as an essential ingredient in the proportion indicated, the health functional food of the present invention may contain food pharmaceutically acceptable food supplement additives, such as natural carbohydrates and various flavoring agents, as additional ingredients. Examples of the natural carbohydrate include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and conventional sugars such as polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As the flavoring agent, thaumatin, rebaudioside A, glycyrrhizin, saccharin, aspartame, and the like may be used. The ratio of the flavoring agent is generally about 1 to 20 g, preferably about 5 to 12 g per 100 ml of the health functional food of the present invention. In addition to the above, the health functional food of the present invention includes various nutrients, vitamins, minerals, flavoring agents such as synthetic and natural flavoring agents, colorants and thickeners, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloids It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food of the present invention may contain natural fruit juice, fruit juice for the production of fruit juice drinks, vegetable drinks, and the like. These components may be used independently or in combination. The proportion of these additives is generally selected in the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active fraction of the present invention.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as a cosmetic composition.

The cosmetic composition of the present invention can be prepared in the form of, for example, essence, nourishing cream, body lotion, conditioner, shampoo, body cleanser, lotion, skin, mask pack, etc. It is not limited here. Preparation in the above form can be easily carried out according to various manufacturing processes known to those skilled in the art.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an animal feed composition.

The animal is a group of organisms corresponding to plants, mainly ingesting organic matter as nutrients, and has differentiated digestive, embryonic and respiratory organs, specifically echinoderms, crustaceans, molluscs, fish, amphibians, reptiles, birds, may be mammals, preferably echinoderms such as sea urchins or sea cucumbers, arthropods including crustaceans such as crabs, shrimps and lobsters, molluscs such as cephalopods, gastropods or bivalves, red sea bream, sea bream, cod, flounder , fish such as halibut, birds including poultry such as pheasants or chickens, or mammals such as pigs, cattle, and goats.

The composition for animal feed may further include grains, vegetable protein feed, animal protein feed, sugar or dairy products to the antimicrobial composition comprising the antimicrobial component of the present invention. The grain may be specifically pulverized or crushed wheat, oats, barley, corn and rice, and the vegetable protein feed may specifically include rapeseed, soybean and sunflower as main components, and the animal protein feed is specifically may be blood meal, meat meal, bone meal, and fish meal, and the sugar or dairy product may be a dry component consisting of various types of powdered milk and whey powder.

The composition for animal feed may additionally contain ingredients such as nutritional supplements, digestion and absorption enhancers, vocal tract promoters or disease prevention agents.

The antimicrobial composition included in the composition for animal feed of the present invention may vary depending on the purpose and conditions of use of the feed, for example, 0.1 g to 100 g of the antimicrobial composition may be included based on 1 kg of the finally produced feed.

In addition, the feed composition may be prepared as a hard viscous granulated or granular material depending on the degree of pulverization of the ingredients, and the composition may be supplied as a mesh or formed into a desired separated shape for further processing and packaging, and storage For ease of storage, it may be subjected to pelletization, expansion or extrusion process, and preferably excess water may be removed by drying.

In a preferred embodiment, the antimicrobial composition of the present invention can be applied as an additive for food, cosmetics or animal feed.

The additive is added to impart preservation to the food, cosmetic or animal feed used, and the antibacterial composition is a natural ingredient and is not harmful to the human body, so it can be safely applied to food, cosmetics or animal feed.

The additive may further include additional other additives appropriately selected according to the purpose and purpose of use, and may be used by mixing with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The additive may be manufactured by powdering, granulating, tableting or liquefying according to the purpose and use thereof, and a conventional method for packaging may be used for productization.

In addition, the amount of the antimicrobial composition used among the additives may be appropriately used depending on the purpose of use, application form, application site, application target article, etc., for example, it may include 0.01 parts by weight to 50 parts by weight based on the total composition, but The content of the composition is not limited thereto. Since the antibacterial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

In a preferred embodiment, the present invention can be applied as an antibacterial composition for use as a cleaning agent.

The cleaning agent may be preferably a detergent for clothes, a dishwashing agent, a food cleaner, and a household appliance cleaner. Since the antibacterial composition is a natural component, it is not harmful to the human body, and more preferably, it can be used for the purpose of washing various household items including kitchenware or food washing or for imparting antibacterial properties.

The detergent may further include an additive selected appropriately according to the purpose and purpose of its use, and may be used by mixing with other active ingredients such as commonly used detergents or additives such as pigments, surfactants, and preservatives. The detergent may be prepared by powdering, granulating, tableting or liquefying according to the purpose and use thereof, and a conventional method for packaging may be used for productization.

In addition, the amount of the antimicrobial composition used in the cleaning agent may be appropriately used depending on the purpose of use, application form, application site, application target article, etc., and may include, for example, 0.01 parts by weight to 50 parts by weight based on the total composition, but The content of the composition is not limited thereto. Since the antimicrobial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts if the application purpose can be achieved due to the characteristics of the product.

In a preferred embodiment, the present invention can be applied as an antibacterial composition for use as a cleaning agent.

The cleanser may be used as a hand, oral or feminine cleanser in a simple way in order to prevent infection of pathogenic microorganisms that are easily infected in daily life in a simple way. Since the antibacterial composition is a natural component and is not harmful to the human body, it can be safely applied even when used as a hand, oral, and feminine cleanser.

The cleaning agent may further include additional additives appropriately selected according to the purpose and purpose of use, and may be used by mixing with other active ingredients such as commonly used additives or additives such as pigments, surfactants, and preservatives. . The cleaning agent may be manufactured by powdering, granulating, tableting, or liquefying depending on the purpose and use thereof, but in the case of a liquid type, convenient use will be possible.

In addition, the amount of the antibacterial composition used in the cleaning agent may be appropriately used depending on the purpose of use, application form, application site, etc., for example, it may include 0.01 parts by weight to 50 parts by weight based on the total composition, but the content of the antibacterial composition is The present invention is not limited thereto. Since the antibacterial composition of the present invention is a natural component and is not harmful to the human body, it can be used in various amounts as long as the application purpose can be achieved due to the characteristics of the product.

Hereinafter, the present invention will be described in more detail through examples. The following examples are only preferred embodiments of the present invention, and the scope of the present invention is not limited to the scope of the following examples.

[Example]

Example 1: Preparation of extracts for each part of ginseng and analysis of their useful components

In 2018, the roots, leaves, and flower buds of 4-year-old ginseng were collected in Yeongju, Gyeongbuk, and dried with hot air (60° C.) in the usual way before use. The ginseng fruit was harvested in July, and the collected seeds were put in a mesh bag, soaked in running water for about a day, then rubbed with hands to completely remove the pulp, and then dried in the shade for 1 day (FIG. 1). Each ginseng part was coarsely pulverized and extracted with ethanol (95%), 10 times the weight of the sample was added with ethanol, extracted three times at room temperature, the extract was collected, filtered, and concentrated under reduced pressure to make a powder, and the ethanol extract was prepared. On the other hand, the ethanol extract of ginseng seed was divided into an upper oil part and a lower layer sediment part during concentration under reduced pressure, and these were recovered respectively. In addition, the above ginseng seeds were machine milked to separately recover ginseng seed oil.

[Table 1] Recovery rate of extracts and milking oil obtained from each part of ginseng

The recovery rate of the ethanol extract for each part of ginseng, as shown in Table 1, was in the order of seeds (8.4%) > leaves (4.3%) > flower buds (1.3%) > roots (0.3%), and ginseng In the case of machine milking of seeds, the recovery rate was about 5%. The ginseng seed mechanically extracted oil was dark brown oil, but the oil produced in the upper layer after concentration of the ethanol extract of ginseng seed under reduced pressure showed a bright yellow color. About 1.3 g of ginseng bud extract could be recovered for 100 g of ginseng flower bud, and the characteristic pungent taste of ginseng flower hardly appeared.

Example 2: Analysis of useful components of extracts and oils obtained from each part of ginseng

Total polyphenols, total flavonoids, total sugars and reducing sugar contents were measured for the samples obtained in Example 1. For the total polyphenol content, 50 μl of Folin-ciocalteau and 100 μl of Na ₂ CO ₃ saturated solution were added to 400 μl of the extraction sample solution, and the absorbance was measured at 725 nm after standing at room temperature for 1 hour. As a standard reagent, tannic acid was used. For the total flavonoid content, each sample was extracted with methanol for 18 hours, and 4 ml of 90% diethylene glycol was added to 400 μl of the filtered extraction sample, 40 μl of 1 N NaOH was added again, and the absorbance was measured at 420 nm after reaction at 37° C. for 1 hour. . As a standard reagent, rutin was used. Reducing sugar was quantified by DNS method and total sugar was quantified by phenol-sulfuric acid method.

[Table 2] Analysis of useful components of extracts and oils for each part of ginseng

As shown in Table 2, as a result of analysis of the useful components of extracts and oils by various parts of ginseng, the total polyphenol content was the highest in the ginseng leaf extract at 22.5 mg/g, leaf extract > bud extract > root Extract ≒ sediment of seed extract > supernatant oil of seed extract > seed milking oil. As a result of measuring the total flavonoid content, ginseng leaf extract showed the highest 13.4 mg/g, and leaf extract > flower bud extract > root extract > seed extract sediment > seed extract upper oil ≒ seed milking oil. In the case of total sugar and reducing sugar content, leaf extract > bud extract > sediment of seed extract > root extract > oil of the upper layer of seed extract ≒ seed milking oil was shown in the following order. These results show that it is possible to develop functional materials using ginseng leaves and flower buds that are discarded without any special use.

Example 3: Evaluation of antibacterial activity of extracts and oils for each part of ginseng

The antibacterial activity of the ginseng extract and oils prepared in Example 1 was evaluated, and the results are shown in Table 3. As strains used for the evaluation of antibacterial activity, Escherichia coli KCTC 1682, Proteus vulgaris KCTC 2433, Pseudomonas aeruginosa KACC 10186, Salmonella typhimurium KCTC 1926 were used as Gram-negative bacteria, and Staphylococcus aureus KCTC 1916, Staphylococcus epidermidis ATCC 12228, Staphylococcus epidermidis were used as Gram-positive bacteria. Listeria monocytogenes KACC 10550 and Bacillus subtilis KCTC 1924 were used. Meanwhile, for the evaluation of antifungal activity, Saccharomyces cerevisiae IF0 0233 and Candida albicans KCTC 1940, a causative agent of Candida fungal infection, were used.

First, in the case of antibacterial activity evaluation, each bacteria was inoculated in Nutrient broth (Difco Co., USA) and cultured at 37° C. for 24 hours, and then each strain _{was adjusted to OD 600} 0.1 to Nutrient agar (Difco Co. , USA) 100 μl of each sample is smeared on a sterile petri dish (90 mm, Green Cross, Korea) containing the medium, and 5 μl of each sample is added to sterile disc-paper (diameter 6.5 mm, Whatsman No. 2), at 37° C. for 24 hours. In the case of fungi, the antibacterial activity was evaluated by measuring the size of the growth inhibition ring after incubation for 24 hours at 30°C in the same manner using Sabouraud dextrose (Difco Co. USA). As a control, the antibacterial ampicillin and the antifungal myconazole (miconazole, Sigma Co., USA) were each used at a concentration of 1 μg/disc. It was measured in mm, and after evaluation three or more times, it is shown as a representative result.

[Table 3] Antibacterial activity of extracts and oils for each part of ginseng

As a result, as shown in Table 3, ampicillin, which is used as an antibacterial agent in clinical practice, showed a wide range of antibacterial activity even at 1 μg/disc, and showed relatively weak growth inhibitory rings (9-15 mm) against Pseudomonas aeruginosa and Salmonella typhimurium. It was. Miconazole, used as an antifungal agent in clinical practice, strongly inhibited the growth of Saccharomyces cerevisiae and Candida albicans even at 1 μg/disc. On the other hand, the ginseng bud extract showed excellent growth inhibition against gram-positive bacteria Listeria monocytogenes , Bacillus subtilis , Staphylococcus epidermidis , Staphylococcus aureus and gram-negative bacteria Proteus vulgaris at a concentration of 500 μg/disc.

Example 4: Evaluation of Plasma, Acid and Thermal Stability of Ginseng Bud Extract

Plasma stability, thermal stability and acid stability for antibacterial activity were confirmed for the ethanol extract of ginseng bud obtained in Example 1. As a result, the active extract showed no significant loss of antibacterial activity even after 1 hour heat treatment at 100° C., 1 hour treatment at pH 2 (0.01M HCl), and 1 hour treatment in plasma. Therefore, the ethanol extract of ginseng bud is expected to maintain excellent antibacterial activity even under various food processing processes.

Claims (5)

Ginseng flower bud (Flower bud of Panax ginseng CA Meyer) containing ethanol extract as an active ingredient, Staphylococcus aureus , Staphylococcus epidermidis , Listeria monocytogenes ) and Bacillus subtilis ( Bacillus subtilis ) Antibacterial composition against Gram-positive bacteria selected from the group consisting of. delete delete delete According to claim 1, wherein the antibacterial composition against Gram-positive bacteria is selected from the group consisting of health functional food, cosmetic composition, animal feed composition, food additive, cosmetic additive, animal feed additive, detergent and cleaning agent. An antibacterial composition for Gram-positive bacteria, characterized in that it is.

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