KR20190019660A - A composition for preventing, improving or treating alcoholic gastro-intestinal disease comprising the extracts from licorice and dandelion - Google Patents

Abstract

The present invention relates to a composition for preventing, improving or treating an alcoholic gastrointestinal tract disease containing licorice and yellow dandelion extract as an active ingredient, and more particularly, to a composition for preventing, improving or treating an alcoholic gastrointestinal tract disease, A pharmaceutical composition for preventing, ameliorating or treating alcoholic gastrointestinal diseases, and a health functional food using the mixture of licorice and yellow dandelion extracts.

Description

TECHNICAL FIELD The present invention relates to a composition for preventing, improving or treating an alcoholic gastrointestinal tract disease containing an extract of licorice and yellow dandelion as an active ingredient.

The present invention relates to a composition for preventing, improving or treating an alcoholic gastrointestinal tract disease containing licorice and yellow dandelion extract as an active ingredient, and more particularly, to a composition for preventing, improving or treating an alcoholic gastrointestinal tract disease, A pharmaceutical composition for preventing, ameliorating or treating alcoholic gastrointestinal diseases, and a health functional food using the mixture of licorice and yellow dandelion extracts.

In Korea, the drinking rate of men over 20 years old is very high (83.3%), and the rate of women drinking is also rapidly increasing to 54.9%, which is seriously exposed to excessive drinking due to wrong drinking culture. The harmful effects of excessive alcohol intake are not only a cause of various diseases such as gastritis and liver disease, but also have a negative impact on the emotions and lives of individuals, families and society, resulting in enormous national and economic losses.

Alcoholic gastritis refers to a state in which the gastric mucosa is damaged by chronic stimuli due to alcohol. Alcoholic gastritis is not easy to digest and sometimes feel sore, but most of the symptoms are not specific symptoms through endoscopy and ultrasonography is found. Chronic drinking does not only lead to gastritis, but also damage the pancreas and liver, leading to related diseases, and also increases the risk of esophageal cancer, stomach cancer and colon cancer.

In particular, gastritis and gastric ulceration due to alcohol require the most attention. Alcohol stimulates the cells of the gastric wall to increase acid secretion. Excessive alcohol consumption causes edema of the gastric mucosa, leading to bleeding and induction of inflammatory cells . In addition, it induces necrosis or cell death due to oxidative stress, damaging the gastric mucosa and causing gastrointestinal bleeding.

Conventional treatments for gastritis and gastric ulcers include antacids that neutralize excess secreted acid, histamine antagonists to inhibit acid secretion, proton pump inhibitors, cholinergic inhibitors, and resistance to gastric lining And gastric mucosal protective agents that help increase recovery. However, such therapeutic agents are synthetic drugs and have side effects such as loss of appetite, weakness, and allergy.

Accordingly, Korean Patent Laid-Open No. 10-2009-0046425 (composition for preventing or treating gastritis or gastric ulcer containing Ganoderma extract), Korean Patent No. 10-1091025 (non-steroidal anti-inflammatory agent containing bokbunja extract Korean Patent Laid-Open No. 10-2012-0051904 (composition for treating gastritis and gastric ulcer including Rhus verniciflua extract), Korean Patent No. 10-1204981 (composition for preventing and treating gastritis containing gonorrhea And compositions for the prevention and treatment of gastric ulcers) have been increasingly attracted to natural extracts having preventive and therapeutic effects of gastritis and gastric ulcer.

Under these circumstances, the inventors of the present invention have confirmed that a mixture of licorice and yellow dandelion extracts has a synergistic effect on the alleviation of alcoholic gastrointestinal diseases, and completed the present invention.

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a composition for preventing, ameliorating or treating alcoholic gastrointestinal tract diseases using a mixture of licorice and yellow dandelion extract.

In order to solve the above-mentioned problems, the present invention relates to a method for producing Glycyrrhiza the present invention provides a pharmaceutical composition for preventing or treating an alcoholic gastrointestinal tract disease comprising, as an active ingredient, a mixture of uralensis and Taraxacum officinale extract.

The present invention also relates to the use of Glycyrrhiza uralensis ) and yellow dandelion ( Taraxacum The present invention provides a health functional food composition for preventing or ameliorating an alcoholic gastrointestinal tract disease, which comprises a mixture of an extract of Lactobacillus officinale as an active ingredient.

According to a preferred embodiment of the present invention, the licorice root is a root, and the yellow dandelion is a ground.

According to another preferred embodiment of the present invention, the licorice and yellow dandelion extracts can be extracted with water, a C ₁ to C ₄ alcohol or a mixed solvent thereof.

According to another preferred embodiment of the present invention, the licorice and yellow dandelion extracts may be contained in a weight ratio of 1: 0.25 to 1: 4.

According to another preferred embodiment of the present invention, the alcoholic gastrointestinal disorder may be at least one selected from the group consisting of acute gastric mucosal injury induced by alcohol, gastric ulcer, acute gastritis, and chronic gastritis.

The composition comprising the licorice and yellow dandelion extracts according to the present invention in a specific weight ratio range is useful for preventing, ameliorating or treating alcoholic gastritis or gastric ulcer because of its synergistic effect on alleviating inflammation of gastric tissues damaged by alcohol. In addition, the mixed composition of the present invention has an effect of inhibiting the activity of GOT and GPT in liver tissue and further shows a preventive effect against alcoholic liver damage.

Furthermore, the composition of the present invention is safe as a human body and has few side effects, and is suitable as a material for the development of medicines, quasi-drugs and health functional foods.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a photograph showing the degree of inflammation relief according to the treatment of a composition in which licorice and yellow dandelion extract are mixed at various weight ratios in gastric mucosa induced gastritis by alcohol administration.
FIG. 2 is a graph showing the incidence of gastric inflammation in each group as a percentage, based on the results of FIG. 1; FIG.
FIG. 3 is a graph showing the effect of suppressing the expression of iNOS (inducible nitric oxide synthase) protein by treatment of a composition comprising licorice and yellow dandelion extract at various weight ratios in rat gastric tissues induced by gastric administration by alcohol administration.
FIG. 4 is a graph showing the inhibitory effects of GOP values (left) and GPT values (right) according to treatment of a composition in which licorice and yellow dandelion extracts were mixed at various weight ratios in rats caused by alcohol administration.

Hereinafter, the present invention will be described in more detail.

As described above, conventional therapeutic agents for gastritis and gastric ulcer include antacid that neutralizes excess secreted acid, histamine antagonist for suppressing acid secretion, proton pump inhibitor, choline inhibitor, And gastric mucosal protective agents to increase the tolerance of gastric lining and to help recovery. However, such therapeutic agents are synthetic medicines, and side effects such as loss of appetite, helplessness, allergy, and the like occur. Therefore, development of gastritis or gastric ulcer treatment using natural extracts without side effects is continuously required.

Accordingly, the present inventors sought a solution to the above-mentioned problem by providing a composition containing a mixture of licorice and a yellow dandelion extract as an active ingredient. The composition comprising the licorice and yellow dandelion extracts according to the present invention in a specific weight ratio range is useful for preventing, ameliorating or treating alcoholic gastritis or gastric ulcer because of its synergistic effect on alleviating inflammation of gastric tissues damaged by alcohol. In addition, the mixed composition of the present invention has an effect of inhibiting the activities of GOT and GPT in liver tissues damaged by alcohol, and can further improve alcoholic liver damage.

The terms used in the present invention are defined as follows.

The term " pharmaceutical composition " means a mixture of a mixture of licorice and yellow dandelion extract of the present invention with other chemical components such as diluent or carrier.

The term " carrier " is defined as a compound that facilitates the addition of a compound into a cell or tissue. For example, dimethylsulfoxide (DMSO) is a commonly used carrier that facilitates the introduction of many organic compounds into cells or tissues of an organism.

The term " diluent " is defined as a compound that not only stabilizes the biologically active form of the compound of interest, but also dilutes in water to which the compound is dissolved. Salts dissolved in buffer solutions are used as diluents in the art. A commonly used buffer solution is phosphate buffered saline, since it mimics the salt state of the human solution. Since buffer salts can control the pH of the solution at low concentrations, buffer diluents rarely modify the biological activity of the compounds.

The term " treatment " means an approach to obtaining beneficial or desired clinical results. For purposes of the present invention, beneficial or desired clinical results include, but are not limited to, alleviation of symptoms, reduction in the extent of disease, stabilization (i.e., not worsening) of the disease state, (Either partially or totally), detectable or undetected, whether or not an improvement or temporary relief or reduction Also, " treatment " may mean increasing the survival rate compared to the expected survival rate when not receiving treatment. &Quot; Treatment " refers to both therapeutic treatment and prophylactic or preventative measures. Such treatments include treatments required for disorders that have already occurred as well as disorders to be prevented. "Alleviating" a disease may result in a reduction in the extent of the disease state and / or undesirable clinical symptoms and / or a slower or longer time course of the progression, It means to lose.

All technical terms used in the present invention are used in the sense that they are generally understood by those of ordinary skill in the relevant field of the present invention unless otherwise defined. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

The present invention relates to the use of Glycyrrhiza uralensis ) and yellow dandelion ( Taraxacum The present invention also provides a pharmaceutical composition for preventing or treating an alcoholic gastrointestinal tract disease, which comprises a mixture of an extract of Lactobacillus officinale as an active ingredient.

In addition, the present invention relates to the use of Glycyrrhiza uralensis ) and yellow dandelion ( Taraxacum The present invention provides a health functional food composition for preventing or ameliorating an alcoholic gastrointestinal tract disease, which comprises a mixture of an extract of Lactobacillus officinale as an active ingredient.

Licorice means a perennial herbaceous plant belonging to the genus Glycyrrhiza, and the licorice used in the present invention is a plant of the genus Glycyrrhiza uralensis ), Manju liquorice ( Glycyrrhiza uralensis Fische) and European licorice ( Glycyrrhiza glabra Linne) is a new varieties that complement the functionality. Rice is stronger than Manchuria licorice and its stems are stronger than those of Manchuria licorice, and its yield is higher than that of Manchuria licorice because it has many roots.

Dandelion is a perennial herbaceous plant belonging to the dicotyledon of a herbaceous plant. Flowers are bloomed in April-May, and young leaves have been edible as herbs and herbs (Kim Il-hyuk and Seonghwan Gil, Medicinal Plums and Trees, Chungang University Press, p332, 1997). Dandelion has been used for the treatment of gangjeong, fever, diuretic, dryness, goddam, and detoxification in one room (Hong Sukhwa, Korea) 101 species of native species, Woongjin Publishing Co., 1990, 18). The taste and nature of dandelion is sweet and sweet, and it is cold. The outpost has a dysfunction and accelerates secretion of gastric juice. In the present invention, yellow dandelion ( Taraxacum officinale ) was used.

The licorice extract and yellow dandelion extract can be obtained by extracting from various organs or parts (for example, leaves, flowers, roots, stems, branches, husks and seeds). In the present invention, the licorice extract is preferably prepared from the roots of licorice root as a raw material, and the yellow dandelion extract is preferably prepared from the ground part of yellow dandelion as a raw material. The term " above ground " refers to leaves and stem parts from which flowers and roots have been removed.

The term "extract" of the present invention refers to an extract obtained by extracting the licorice or yellow dandelion, a dilute or concentrated solution of the extract, a dried product obtained by drying the extract, a preparation or a purified product of the extract, Etc., the extract itself and extracts of all the formulations which can be formed using the extract. Specifically, the extract of the present invention may be prepared in the form of a dry powder after extraction.

The extract of the present invention can be extracted from natural, hybrid or variant plants of the plant, and can also be extracted from plant tissue cultures.

In the extraction of licorice or yellow dandelion of the present invention, the extraction method is not particularly limited, and extraction can be carried out according to a method commonly used in the art. Non-limiting examples of the extraction method include hydrothermal extraction, ultrasonic extraction, filtration, and reflux extraction. These may be performed alone or in combination with two or more methods.

In the present invention, the kind of the extraction solvent used for extracting licorice or yellow dandelion is not particularly limited, and any solvent known in the art can be used. Non-limiting examples of the extraction solvent include water; C ₁ -C ₄ lower alcohols such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol and propylene glycol; And hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether, and dichloromethane; Or mixtures thereof. Specifically, the licorice or yellow dandelion can be extracted with water, a C ₁ to C ₄ alcohol, or a mixed solvent thereof, but is not limited thereto. In the present invention, the lower alcohol may be ethanol, but is not limited thereto.

In one embodiment of the present invention, the ground portion of the licorice root and the yellow dandelion having the foreign substances removed by washing and drying were lyophilized, pulverized, and then subjected to hot water extraction by repeating three times with water. If necessary, the powder may be further subjected to steps such as filtration, concentration and freeze-drying according to methods known in the art.

In one embodiment of the present invention, in order to confirm the effect of improving the alcoholic gastritis of the composition containing the mixture of licorice and yellow dandelion extract of the present invention, in a rat animal model in which gastric inflammation is caused by administration of alcohol, preferably ethanol, Histopathological evaluation and area of inflammation were analyzed.

As shown in Figs. 1 and 2, in the case of the ethanol-only control group, inflammation, hemorrhage, erythema and edema of the stomach occurred and the area of the damaged area was also very wide. In the case of administration of the single extract, the effect of improving the stomach inflammation of the yellow dandelion extract was superior to that of the licorice extract. It was confirmed that there was a specific synergistic effect in the specific weight ratio in the experimental group administered with the mixture A, B and C, respectively, administered in the same amount as that of the single extract administration group.

Specifically, the compound synergistic effect was increased as the weight ratio of the yellow dandelion extract to the mixture was increased. The preferred mixing weight ratio is 1: 0.25 to 1: 4, more preferably 1: 1 to 1: 4, and most preferably 1: 4. In particular, when the weight ratio of the licorice extract to the yellow dandelion extract is in the range of 1: 1 to 1: 4, the effect of improving the stomach inflammation in the single administration group of licorice extract or yellow dandelion extract is exerted, Effect was observed.

When the licorice and yellow dandelion extracts are mixed at a weight ratio out of the above range, the complex synergistic effect of the two extracts is reduced and the optimum activity can not be exhibited (FIGS. 1 and 2) .

In addition, as shown in FIG. 3, it was confirmed that the expression of iNOS was significantly inhibited in the mixture B and C of the experimental groups to which the mixtures A, B and C were respectively administered, and in particular, the licorice extract and the yellow dandelion extract had a weight ratio of 1: 4 , The inhibitory effect on iNOS expression was maximized in the mixture C group.

Therefore, the mixture of the licorice extract and the yellow dandelion extract at a weight ratio of 1: 1 to 1: 4 can remarkably alleviate the inflammation of the stomach tissue induced by alcohol, and thus has an excellent effect in the treatment of alcoholic gastritis.

In the present invention, " Alcoholic Gastrointestinal Disease " refers to any disease caused by alcohol-induced stomach injury. For example, it may be at least one selected from the group consisting of acute gastric mucosal injury induced by alcohol, gastric ulcer, acute gastritis and chronic gastritis, but is not limited thereto.

In addition, the mixture of licorice extract and yellow dandelion extract of the present invention can exhibit a preventive effect against alcoholic liver damage.

In order to distinguish the various diseases related to the liver, it is necessary to comprehensively analyze several chemical tests. For this purpose, some test items are collectively referred to as liver function tests. Major tests include GOT and GPT. In addition, there are many cases in which total protein, albumin, and ammonia are added to the test.

GOT (glutamic oxaloacetic transaminase) is an enzyme that catalyzes the reversible reaction of producing oxaloacetic acid and L-glutamic acid by transferring the amino group of L-aspartic acid to? -Ketoglutaric acid. In the diagnosis of hepatitis and myocardial infarction, measurement of this enzyme activity in the blood is frequently used.

GPT (glutamic-pyruvic transaminase) is an enzyme that catalyzes pyruvic acid and glutamic acid production by transferring the amino group of alanine to α-keto glutaric acid. In order to diagnose liver and biliary system diseases, the activity of this enzyme in blood is frequently measured.

Therefore, in the present invention, the improvement effect on alcoholic liver damage was confirmed by GOT and GPT measurement. As shown in FIG. 4, it was confirmed that the composition containing the mixture of the licorice extract and the yellow dandelion extract of the present invention markedly decreased the GOT and GPT levels, which were increased by the administration of alcohol, preferably ethanol. The remarkable synergistic effect of the group administered with the mixture appears to be due to the fact that the administered mixture is absorbed more rapidly than ethanol and reduces the GOT and GPT levels.

From the above results, it can be seen that the mixture of the licorice extract and yellow dandelion extract of the present invention not only improves the stomach damage caused by alcohol but also shows the effect of preventing liver damage, so that the stomach and liver can be protected simultaneously from the excessive consumption of alcohol.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents conventionally used in the manufacture of pharmaceutical compositions.

The pharmaceutical composition according to the present invention can be formulated in the form of oral, granule, tablet, capsule, suspension, emulsion, syrup, aerosol or the like oral preparation, external preparation, suppository and sterilized injection solution according to a conventional method Can be used.

Examples of carriers, excipients and diluents that may be contained in the composition of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate, calcium silicate, cellulose, methylcellulose, microcrystalline cellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

In the case of formulation, a diluent or excipient such as a filler, an extender, a binder, a wetting agent, a disintegrant, or a surfactant is usually used.

Solid formulations for oral administration include tablets, pills, powders, granules, capsules and the like, which may contain at least one excipient such as starch, calcium carbonate, sucrose or lactose , Gelatin, and the like. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Examples of the liquid preparation for oral use include suspensions, solutions, emulsions, and syrups. In addition to water and liquid paraffin, simple diluents commonly used, various excipients such as wetting agents, sweeteners, fragrances, preservatives and the like may be included .

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Examples of the suspending agent include propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate, and the like. Examples of the suppository base include witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin and the like.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount.

The term "pharmaceutically effective amount " as used herein means an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level will vary depending on the species and severity, age, sex, , The activity of the drug, the sensitivity to the drug, the time of administration, the route of administration and rate of release, the duration of the treatment, factors including co-administered drugs, and other factors well known in the medical arts. The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. And can be administered singly or multiply. It is important to take into account all of the above factors and to administer the amount in which the maximum effect can be obtained in a minimal amount without adverse effect, and can be easily determined by those skilled in the art.

The pharmaceutical composition of the present invention can be used alone or in combination with methods using surgery, hormone therapy, drug therapy and biological response modifiers.

The composition can be administered to mammals such as rats, mice, livestock, humans, and the like in a variety of routes. All modes of administration may be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intra-uterine dural or intracerebral injection.

When the mixture of licorice extract and yellow dandelion extract of the present invention is used as a food additive, the mixture can be used as it is, or can be used together with other food or food ingredients, and can be suitably used according to a conventional method. The amount of the active ingredient to be mixed can be suitably determined according to the intended use (prevention, health or therapeutic treatment). Generally, in the production of food or beverage, the composition of the present invention is added in an amount of 0.01 to 10% by weight, preferably 0.05 to 1% by weight based on the raw material. However, in the case of long-term ingestion intended for health and hygiene purposes or for the purpose of controlling health, the amount can also be used in the above-mentioned range.

The health functional food may contain flavoring agents such as various nutrients, vitamins, minerals (electrolytes), synthetic flavors and natural flavors, coloring agents and thickening agents (cheese, chocolate etc.), pectic acid and its salts, alginic acid and its salts, Organic acids, protective colloid thickeners, pH adjusting agents, stabilizers, preservatives, glycerin, alcohols, carbonating agents used in carbonated drinks, and the like. It can also contain natural fruit juices and pulp for the production of fruit juices and vegetable drinks. These components may be used independently or in combination. The health functional food may be any one of meat, sausage, bread, chocolate, candy, snack, confectionery, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcohol and vitamin .

The health beverage composition of the present invention is not particularly limited to a liquid ingredient except that it contains a mixture of licorice extract and yellow dandelion extract, and may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages.

In addition, the health functional food may further include food additives, and the suitability of the food functional food as a " food additive " is not limited to the corresponding items in general rules and general test methods approved by the Food and Drug Administration Shall be determined according to the relevant standards and standards.

Examples of the products that have been used in the above-mentioned "food additives" include natural products such as ketones, chemical products such as glycine, potassium citrate, nicotinic acid and cinnamic acid, sensory coloring matter, licorice extract, crystalline cellulose, high- - Mixed preparations such as a sodium glutamate preparation, a noodle-added alkaline agent, a preservative preparation, a tar coloring agent and the like.

The present invention will now be described more specifically with reference to the following examples. However, the following examples should not be construed as limiting the scope of the present invention, but should be construed to facilitate understanding of the present invention.

1-1. Preparation of Extracts of Licorice Root Extract and Yellow Dandelion

The ground portion of the harvested licorice root and yellow dandelion was washed with water and lyophilized with a freeze dryer (Martin Christ, Osterode, Germany). After lysing the lyophilized sample, 1,000 ml of water was added to 150 g of each sample, and the mixture was subjected to hot extraction three times for 3 hours. The extracts were removed with insoluble materials using Whatman 46 cm filter paper, and the filtered extracts were concentrated under reduced pressure at 60 ° C with a rotary evaporator (Heidolph, Germanay). In order to completely remove the solvent of the concentrated extract, the supernatant was suspended in 50 ml of purified water, and 27.5 g of the lyophilized root extract-extracted freeze-dried product and 61.3 g of the yellow dandelion ground freeze-dried extract were obtained using a freeze dryer (yield of licorice extract: 18.3% , Yield of yellow dandelion extract: 40.9%).

1-2. Experimental animal

Seven-week-old Spraque-Dawley male rats were purchased from Orient Bio. The light / dark cycle was set at 12 hours at 22 ° C, and the diet and water were freely fed and adapted to the basic diet for one week.

Identification of alcoholic gastritis improvement

2-1. Experimental set-up and sample administration

The experimental animals of Example 1-2 were divided into five groups, normal control group, 5.0 g / kg ethanol alone, rebamipide as a gastritis treatment, 5.0 g / kg The experimental group was divided into five experimental groups, each of which was administered with a test group dissolved in ethanol and a test group dissolved in 5.0 g / kg of ethanol. The information of the experimental samples administered for the five experimental groups is as shown in Table 1 below.

Experimental animals were fasted for 12 hours. The experimental samples were dissolved in ethanol (5.0 g / kg) and then orally administered once. After 1 hour, the animals were anesthetized with ether, and cardiac blood collection and stomach tissues were extracted.

Experimental group Experimental sample Licorice Root Extract Yellow dandelion
Above Ground Extract Licorice treated group 200 mg / kg - Yellow dandelion group - 200 mg / kg Mixture A administration group 160 mg / kg 40 mg / kg Mixture B administration group 100 mg / kg 100 mg / kg Mixture C administration group 40 mg / kg 160 mg / kg

2-2. Histopathological evaluation of stomach tissue and analysis of inflammation area

The stomach tissues extracted from each group were fixed and the degree of damage and degree of damage were observed. As a result, as shown in Fig. 1, in the case of the control group in which only ethanol was administered, inflammation, hemorrhage, erythema and edema occurred in the stomach tissue and the area of the damaged area was also very wide. These stomach injuries were noticeably restored in Mixture B and C treatment groups.

As shown in FIG. 2, the areas of inflammation in the licorice and yellow dandelion groups were 43.3% and 31.0%, respectively. As shown in FIG. 2, the area of inflammation was 43.3% and 31.0% The area of inflammation in the mixture C administration group was 41.9%, 29.6% and 20.2%.

As a result, it was confirmed that the synergistic effect was maximized in the mixture C group in which the weight ratio of 1: 4 of the licorice root extract and the yellow dandelion root extract was higher than that of the other experimental groups, and the gastric tissue damage was remarkably alleviated.

2-3. Confirming the effect of inhibiting the expression of inflammatory proteins in gastric tissues

The amount of protein was measured by Western blot method to evaluate the expression of inflammation - related protein (iNOS) by administration of mixture of licorice root extract and yellow dandelion root extract.

10 [mu] g of protein was separated by electrophoresis using SDS-polyacrylamide gel, and the acrylamide gel was transferred to a nitrocellulose membrane. The cells were then immersed in TBS-T (0.5% Tween 20 dissolved in TBS) containing 5% skim milk for 1 hour and then washed 5 times every 5 minutes with TBS-T. The primary antibodies were treated with the prepared membranes and incubated overnight at 4 ° C and then washed five times with TBS-T every 10 min. The reaction was performed at room temperature for 1 hour and 30 minutes using a secondary antibody (diluted 1: 5000 with TBS-T) to the treated primary antibody, and then washed five times with TBS-T every 10 minutes. ECL solution and exposed to Davinch-ChemiTM Chemiluminescence Imaging System (Davinch-K Co., Ltd., Seoul, Korea) to confirm the expression of the protein. Then, the band was subjected to ATTO Densitograph Software (ATTO Corporation, Tokyo, Japan) .

As a result, as shown in FIG. 3, it was confirmed that the expression of iNOS was significantly suppressed in the mixture group B and C in the experimental group, and in particular, in the mixture C group in which the licorice root extract and the yellow dandelion root extract were mixed at a weight ratio of 1: and iNOS expression inhibition effect was maximized.

From the above results, it was confirmed that a composition in which the licorice root extract and the yellow dandelion ground extract are mixed at a weight ratio of 1: 1 to 1: 4 is very useful for treating alcoholic gastritis.

Measurement of serum liver function index enzyme activity

The activity of glutamic-pyruvic transaminase (GOT) and glutamic-pyruvic transaminase (GOT) in the blood obtained from the experimental animal of Example 2-1 was measured using a diagnostic kit (Procedure No. 505, Sigma Chemical Co., St. Louis, , ≪ / RTI > MO) as colorimetrically.

As a result, as shown in FIG. 4, the GOT and GPT values were increased by ethanol administration (control group), and the GOT and GPT values of the mixture group A, B and C groups were higher than those of the licorice and yellow dandelion alone groups . The remarkable synergistic effect of the mixture administration group appears to be due to the fact that the administered mixture is absorbed more rapidly than ethanol to reduce the GOT and GPT levels. Thus, the mixture of the licorice root extract of the present invention and the yellow dandelion topsoil extract, Can be effectively prevented.

Preparation Example 1: Preparation of pharmaceutical preparations

1-1. Manufacture of Powder

A mixture of 0.5 g of the licorice root extract and 2.0 g of the yellow dandelion root extract or 1.25 g of the licorice root extract and 1.25 g of the yellow dandelion root extract was mixed with 1 g of lactose and filled in airtight bags to prepare powders.

1-2. Manufacture of tablets

A mixture of 25 mg of the licorice root extract and 100 mg of the yellow dandelion root extract or 62.5 mg of the licorice root extract and 62.5 mg of the yellow dandelion root extract was mixed with 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate, Tablets were prepared by tabletting according to the manufacturing method.

1-3. Preparation of capsules

A mixture of 0.5 g of the licorice root extract and 2.0 g of the yellow dandelion ground extract, or a mixture of 1.25 g of the licorice root extract and 1.25 g of the yellow dandelion ground extract was mixed with 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate, The capsules were filled in gelatin capsules according to the preparation method of capsules.

1-4. Injection preparation

A mixture of 2.5 g / ml of licorice root extract and 10 .mu.g / ml of yellow dandelion extract or 6.25 .mu.g / ml of licorice root extract and 6.25 .mu.g / ml of yellow dandelion root extract was dissolved in suitable injectable sodium chloride BP and the resulting solution The pH was adjusted to pH 3.5 with diluted hydrochloric acid BP and mixed well by adjusting the volume using injectable sodium chloride BP (up to 1 ml). The solution was filled in a 5 ml type I ampoule made of transparent glass, sealed in an upper lattice of air by dissolving the glass, sterilized by autoclaving at 120 DEG C for 15 minutes or longer, and an injection solution was prepared.

Preparation Example 2: Preparation of health food

2-1. Manufacture of cooking seasonings

A mixture of the licorice root extract and the yellow dandelion ground top extract at a weight ratio of 4: 1 to 1: 4 was added in an amount of 0.2 to 10% by weight to prepare a cooking sauce for health promotion.

2-2. tomato ketchup  And source

0.2-1.0% by weight of a mixture of a licorice root extract and a yellow dandelion ground top extract mixture at a weight ratio of 1: 1 to 1: 4 was added to tomato ketchup or sauce to prepare health improvement tomato ketchup or sauce.

2-3. Manufacture of flour food products

0.1 to 5.0% by weight of a mixture of a licorice root extract and a yellow dandelion ground top extract mixture at a weight ratio of 1: 1 to 1: 4 was added to wheat flour, and bread, cake, cookies, crackers and noodles were prepared Health enhancing foods were prepared.

2-4. Manufacture of soups and gravies

0.1 to 1.0 wt.% Of a mixture of licorice root extract and yellow dandelion ground top extract mixture at a weight ratio of 4: 1 to 1: 4 was added to soup and juice to prepare health promotion meat product, noodle soup and juice.

2-5. Manufacture of ground beef

A ground beef for health promotion was prepared by adding 10% by weight of a mixture of licorice root extract and yellow dandelion ground top extract at a weight ratio of 1: 1 to 1: 4 to ground beef.

2-6. Manufacture of dairy products

0.1 to 1.0% by weight of a mixture of a licorice root extract and a yellow dandelion topsoil extract at a weight ratio of 1: 1 to 1: 4 was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

2-7. Manufacture of wires

The brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the powder was prepared into a powder having a particle size of 60 mesh by a pulverizer.

Black beans, black sesame seeds, and perilla seeds were steamed and dried by a known method, and then they were prepared into powders having a particle size of 60 mesh by a pulverizer.

The mixture obtained by mixing the licorice root extract and the yellow dandelion ground top extract in a weight ratio of 1: 1 to 1: 4 was concentrated under reduced pressure in a vacuum concentrator, sprayed and dried with a hot air drier, and the resulting dried product was pulverized into 60- ≪ / RTI >

The above-prepared cereal grains, seeds and dried powder of the licorice extract were blended in the following proportions.

Cereals (30% by weight of brown rice, 15% by weight of yulmu, 20% by weight of barley)

Seeds (7% by weight of perilla, 8% by weight of black beans, 7% by weight of black sesame seeds)

Dried powder (1% by weight) of the mixture in which the licorice root extract and the yellow dandelion topsoil extract were mixed at a weight ratio of 1: 1 to 1: 4,

Manure (0.5% by weight), and

(0.5% by weight)

2-8. Manufacture of carbonated drinks

Syrup was prepared by mixing additives of 5 to 10% of sugar, 0.05 to 0.3% of citric acid, 0.005 to 0.02% of caramel and 0.1 to 1% of vitamin C and 79 to 94% of purified water. The syrup prepared above was sterilized at 85 to 98 ° C for 20 to 180 seconds and mixed with cooling water at a ratio of 1: 4. Then, carbonic acid gas was injected at 0.5 to 0.82%, and the extracts of licorice root extract and yellow dandelion ground- 1 to 1: 4 by weight.

2-9. Manufacture of health drinks

The extracts of licorice root and yellow dandelion ground top extracts were mixed in a ratio of 1: 1 to 1: 4 (1: 1) to fructose such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2% , The mixture was homogenized and sterilized instantaneously, and then packaged in a glass bottle, plastic bottles and plastic bottles to prepare a health drink.

Claims (10)

( Glycyrrhiza uralensis Fischer) and a yellow dandelion ( Taraxacum officinale ) extract as an active ingredient. The present invention also provides a pharmaceutical composition for preventing or treating alcoholic gastrointestinal diseases. [Claim 7] The pharmaceutical composition according to claim 1, wherein the licorice root is a root, and the yellow dandelion is a root. The pharmaceutical composition according to claim 1, wherein the licorice and yellow dandelion extracts are extracted with water, a C ₁ to C ₄ alcohol, or a mixed solvent thereof.  The pharmaceutical composition according to claim 1, wherein the licorice and yellow dandelion extracts are contained in a weight ratio of 1: 0.25 to 1: 4. The pharmaceutical composition according to claim 1, wherein the alcoholic gastrointestinal tract disease is at least one selected from the group consisting of acute gastric mucosal injury induced by alcohol, gastric ulcer, acute gastritis, and chronic gastritis. A health functional food composition for preventing or ameliorating an alcoholic gastritis containing a mixture of licorice ( Glycyrrhiza uralensis Fischer) and yellow dandelion ( Taraxacum officinale ) extract as an active ingredient. The health functional food composition according to claim 6, wherein the licorice root is a root, and the yellow dandelion is a root. The health functional food composition according to claim 6, wherein the licorice and yellow dandelion extracts are extracted with water, a C ₁ to C ₄ alcohol, or a mixed solvent thereof. The health functional food composition according to claim 6, wherein the licorice and yellow dandelion extracts are contained in a weight ratio of 1: 0.25 to 1: 4. [Claim 7] The health functional food composition according to claim 6, wherein the alcoholic gastrointestinal tract disease is at least one selected from the group consisting of acute gastric mucosal injury induced by alcohol, gastric ulcer, acute gastritis and chronic gastritis.

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