KR102019974B1 - A composition for preventing, improving or treating alcoholic gastro-intestinal disease comprising the extracts from licorice and dandelion - Google Patents

Abstract

The present invention relates to a composition for the prevention, improvement or treatment of alcoholic gastrointestinal diseases containing licorice and yellow dandelion extract as an active ingredient, and more particularly, has a synergistic effect in reducing inflammation of alcohol-induced gastric tissue. Using a mixture of licorice and yellow dandelion extract to provide a pharmaceutical composition and health functional food for the prevention, improvement or treatment of alcoholic gastrointestinal diseases.

Description

A composition for preventing, improving or treating alcoholic gastro-intestinal disease comprising the extracts from licorice and dandelion}

The present invention relates to a composition for the prevention, improvement or treatment of alcoholic gastrointestinal diseases containing licorice and yellow dandelion extract as an active ingredient, and more particularly, has a synergistic effect in reducing inflammation of alcohol-induced gastric tissue. Using a mixture of licorice and yellow dandelion extract to provide a pharmaceutical composition and health functional food for the prevention, improvement or treatment of alcoholic gastrointestinal diseases.

In Korea, the drinking rate of males over 20 years of age is very high (83.3%), and the drinking rate of females is also rapidly increasing to 54.9%. The harm caused by excessive intake of alcohol is not only a cause of gastritis and liver disease, but also negatively affects the emotions and lives of individuals, families, and society, causing huge economic and social losses.

Alcoholic gastritis is a condition in which the gastric mucosa is damaged by chronic stimulation caused by alcohol. Alcoholic gastritis is often difficult to digest and complains of a bitter feeling, but most cases have no symptoms, and serious symptoms are found through endoscopy and ultrasound. If you drink alcohol chronically, not only gastritis, but also damage the pancreas and liver, causing related diseases, and also increases the risk of esophageal cancer, stomach cancer, colon cancer.

In particular, gastritis and gastric ulcers caused by alcohol require the most attention, which stimulates the cells of the stomach wall to increase acid secretion, and excessive alcohol intake causes swelling of the gastric mucosa, causing bleeding and inducing the production of inflammatory cells. . In addition, necrosis or apoptosis caused by oxidative stress damages the gastric mucosa, and gastric bleeding appears.

Conventional treatments for gastritis and gastric ulcers include antacids that neutralize excessive secreted gastric acid, histamine antagonists for acid secretion, proton pump inhibitors, choline inhibitors, and gastrointestinal resistance. Gastric mucoprotectants that can help increase and recover. However, these therapeutic agents have a problem that side effects such as loss of appetite, helplessness, allergies, etc. as synthetic drugs.

Accordingly, Republic of Korea Patent Publication No. 10-2009-0046425 (composition for the prevention or treatment of gastritis or gastric ulcer containing cheonma extract), Republic of Korea Patent No. 10-1091025 (non-steroidal anti-inflammatory agent containing bokbunja extract Induced gastritis and gastric ulcer prevention and treatment composition), Republic of Korea Patent Publication No. 10-2012-0051904 (Gastroenteritis and gastric ulcer composition comprising lacquer extract), Republic of Korea Patent No. 10-1204981 (Gastroenteritis containing gorgo sap) And compositions for the prevention and treatment of gastric ulcers), such as natural extracts that have the effect of preventing and treating gastritis and gastric ulcers.

Under this background, the present inventors have confirmed that the mixture of licorice and yellow dandelion extract has a synergistic effect on the alleviation of alcoholic gastrointestinal diseases and completed the present invention.

The present invention has been made to solve the above problems, it is an object to provide a composition for preventing, ameliorating or treating alcoholic gastrointestinal diseases using a mixture of licorice and yellow dandelion extract.

In order to solve the above problems, the present invention is a licorice ( Glycyrrhiza It provides a pharmaceutical composition for the prevention or treatment of alcoholic gastrointestinal diseases containing a mixture of uralensis ) and extracts of Taraxacum officinale as an active ingredient.

The invention also relates to licorice ( Glycyrrhiza uralensis ) and yellow dandelion ( Taraxacum) officinale) provides a mixture of an alcoholic gastrointestinal disease preventing or improving functional health food composition for containing, as an active ingredient of the extract.

According to a preferred embodiment of the present invention, the part of the licorice is the root, the part of the yellow dandelion may be the ground portion.

According to another preferred embodiment of the present invention, the licorice and yellow dandelion extract may be extracted with water, C ₁ ~ C ₄ alcohol or a mixed solvent thereof.

According to another preferred embodiment of the present invention, the licorice and yellow dandelion extract may be contained in a weight ratio of 1: 0.25 to 1: 4.

According to another preferred embodiment of the present invention, the alcoholic gastrointestinal disease may be at least one selected from the group consisting of alcohol-induced acute gastric mucosal injury, gastric ulcer, acute gastritis and chronic gastritis.

The composition of the licorice and yellow dandelion extract according to the present invention mixed in a specific weight ratio range is excellent in synergistic effect on relieving inflammation of gastric tissue damaged by alcohol, and is useful for preventing, improving or treating alcoholic gastritis or gastric ulcer. In addition, the mixed composition of the present invention has the effect of inhibiting the activity of GOT and GPT in liver tissue, further showing a preventive effect against alcoholic liver damage.

Furthermore, the composition of the present invention is safe for the human body using the extract of the natural material as an active ingredient, less side effects are suitable as a material for the development of medicines, quasi-drugs and health functional foods.

Figure 1 is a photograph showing the degree of inflammation relief according to the treatment of the composition of licorice and yellow dandelion extract in various weight ratios in the gastric tissue of the rat gastritis induced by alcohol administration.
FIG. 2 is a graph showing the calculated percentage of gastric inflammation in each group based on the results of FIG. 1.
Figure 3 is a graph showing the effect of inhibiting the expression of inducible nitric oxide synthase (iNOS) protein according to the treatment of a mixture of licorice and yellow dandelion extract in various weight ratios in the gastric tissue of rat gastric inflammation induced by alcohol administration.
Figure 4 is a graph showing the effect of inhibiting the GOP (left) and GPT (right) according to the treatment of a composition mixed with licorice and yellow dandelion extract in various weight ratio in rats causing liver damage by alcohol administration.

Hereinafter, the present invention will be described in more detail.

As described above, conventional therapeutic agents for gastritis and gastric ulcers include antacids that neutralize excessive secreted gastric acid, histamine antagonists for acid secretion, proton pump inhibitors, choline inhibitors, Gastric mucoprotective agents that increase the resistance of the gastric lining and help recovery. However, these therapeutic agents have a problem in that side effects such as loss of appetite, helplessness, and allergies are caused by synthetic drugs, and there is a continuous demand for development of a gastritis or gastric ulcer therapeutic agent using natural extracts without side effects.

Accordingly, the present inventors sought solutions to the above problems by providing a composition containing a mixture of licorice and yellow dandelion extract as an active ingredient. The composition of the licorice and yellow dandelion extract according to the present invention mixed in a specific weight ratio range is excellent in synergistic effect on relieving inflammation of gastric tissue damaged by alcohol, and is useful for preventing, improving or treating alcoholic gastritis or gastric ulcer. In addition, the mixed composition of the present invention has the effect of inhibiting the activity of GOT and GPT in the liver tissue damaged by alcohol, it can further improve alcoholic liver damage.

The terms used in the present invention are defined as follows.

The term “pharmaceutical composition” refers to a mixture of other chemical components such as diluents or carriers in a mixture of licorice and yellow dandelion extracts of the present invention.

The term “carrier” is defined as a compound that facilitates the addition of a compound into a cell or tissue. For example, dimethyl sulfoxide (DMSO) is a commonly used carrier that facilitates the incorporation of many organic compounds into cells or tissues of an organism.

The term “diluent” is defined as a compound that not only stabilizes the biologically active form of the compound of interest, but also is diluted in water to dissolve the compound. Salts dissolved in buffer solutions are used as diluents in the art. A commonly used buffer solution is phosphate buffered saline, because it mimics the salt state of human solutions. Because buffer salts can control the pH of a solution at low concentrations, buffer diluents rarely modify the biological activity of a compound.

The term “treatment” refers to an approach for obtaining beneficial or desirable clinical results. For the purposes of the present invention, beneficial or desirable clinical outcomes include, but are not limited to, alleviation of symptoms, reduction of disease range, stabilization of disease state (ie, not worsening), delay or slowing of disease progression, disease state Improvement or temporary mitigation and alleviation (partially or wholly), detectable or not detected. "Treatment" may also mean increasing survival compared to the expected survival rate without treatment. "Treatment" refers to both therapeutic treatment and preventive or preventive measures. Such treatments include the treatments required for the disorders that have already occurred as well as the disorders to be prevented. “Alleviating” a disease may reduce the extent of disease status and / or undesirable clinical signs and / or slow or lengthen the time course of progression as compared to untreated treatment. It means losing.

All technical terms used in the present invention, unless defined otherwise, are used in the meaning as commonly understood by those skilled in the art in the related field of the present invention. Also described herein are preferred methods or samples, but similar or equivalent ones are within the scope of the present invention.

The present invention is licorice ( Glycyrrhiza uralensis ) and yellow dandelion ( Taraxacum) officinale) provides the alcohol gastrointestinal disease prevention or treatment a pharmaceutical composition containing as an active ingredient of the mixture of the extract.

In addition, the present invention is licorice ( Glycyrrhiza uralensis ) and yellow dandelion ( Taraxacum) officinale) provides a mixture of an alcoholic gastrointestinal disease preventing or improving functional health food composition for containing, as an active ingredient of the extract.

Licorice means a perennial herbaceous plant belonging to the genus Licorice (Glycyrrhiza), the licorice used in the present invention is ' Glycyrrhiza ' developed by the Rural Development Administration uralensis ', Glycyrrhiza uralensis Fische) and European licorice ( Glycyrrhiza glabra Linne) is a new breed that complements the functionality. The persimmon has a higher stem and a stronger root than Manchurian licorice, and the yield is higher than that of Manchurian licorice.

Dandelion is a perennial herbaceous plant belonging to the dicotyledonous genus of the genus Botanical Gate. Flowers bloom in April-May, and young leaves have been edible with herbs and national streets (Kim Il-hyuk and Seonghwan-gil, medicinal grasses and trees, Chung-Ang University Press, p332, 1997). Dandelion is also called pogongyoung (Kim Won, Botanical Botany, Kyungpook National University Press, p190, 1987). 101 native species, Woongjin Press, 1990, 18). Dandelion taste and properties are bitter and sweet. Outpost has an edema and speeds up the secretion of gastric juice. In the present invention, yellow dandelion ( Taraxacum officinale ) was used.

The licorice extract and yellow dandelion extract can be obtained by extracting from various organs or parts (for example, leaves, flowers, roots, stems, branches, shells and seeds, etc.). Licorice extract in the present invention is preferably prepared by using the root portion of licorice, yellow dandelion extract is preferably prepared by using the ground portion of the yellow dandelion. The term "ground" refers to the leaf and stem area from which flowers and roots are removed.

The term " extract " of the present invention refers to an extract obtained by extracting licorice or yellow dandelion, a diluent or pesticide solution of the extract, a dried product obtained by drying the extract, a crude or purified product of the extract, or a mixture thereof. And the like, and extracts of all formulations that can be formed using the extract itself and the extract. Specifically, the extract of the present invention may be prepared and used in the form of a dry powder after extraction.

The extract of the present invention can be extracted from natural, hybrid or variety plants of the plant, it is also possible to extract from plant tissue culture.

In the extraction of the licorice or yellow dandelion of the present invention, the extraction method is not particularly limited and may be extracted according to a method commonly used in the art. Non-limiting examples of the extraction method, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method and the like, these may be carried out alone or in combination of two or more methods.

The kind of the extraction solvent used to extract the licorice or yellow dandelion in the present invention is not particularly limited, and any solvent known in the art may be used. Non-limiting examples of such extraction solvents include water; Lower alcohols such as C ₁ to C ₄ such as methanol, ethanol, propyl alcohol and butyl alcohol; Polyhydric alcohols such as glycerin, butylene glycol, and propylene glycol; Hydrocarbon solvents such as methyl acetate, ethyl acetate, acetone, benzene, hexane, diethyl ether and dichloromethane; Or a mixture thereof may be used, and specifically, the licorice or yellow dandelion may be extracted with water, C ₁ to C ₄ alcohol or a mixed solvent thereof, but is not limited thereto. In the present invention, the lower alcohol may be ethanol, but is not limited thereto.

In one embodiment of the present invention, the licorice root and yellow dandelion ground portion from which foreign substances were removed by washing and drying were lyophilized, pulverized, and extracted with hot water three times. If necessary, it may be prepared as a powder through additional steps such as filtration, concentration and lyophilization according to methods known in the art.

In one embodiment of the present invention, in order to confirm the alcoholic gastritis improvement effect of the composition containing the mixture of licorice and yellow dandelion extract of the present invention of the gastric tissue in a rat animal model in which the gastric inflammation occurred by administration of alcohol, preferably ethanol Histopathological evaluation and inflammation incidence area were analyzed.

As shown in Figures 1 and 2, the control group administered only with ethanol, inflammation, bleeding, redness and edema of the gastric tissue occurred, the damage area was also very large. In the case of the administration of the extract alone, yellow dandelion extract showed better gastric tissue inflammation than the licorice extract. It was confirmed that a specific synergistic effect was observed at a specific weight ratio in the experimental group administered with the mixture A, B and C, respectively, which were administered in the same amount as the single extract administration group but mixed in different weight ratios.

Specifically, the composite synergy was increased as the weight ratio of the yellow dandelion extract in the mixture increased. Preferred mixing weight ratios are 1: 0.25 to 1: 4, more preferably 1: 1 to 1: 4, and the most preferred mixing weight ratio may be 1: 4. In particular, when the mixed weight ratio of licorice extract and yellow dandelion extract is 1: 1 to 1: 4, it shows a superior effect to gastric tissue inflammation improvement effect in the administration group of licorice extract or yellow dandelion extract, so that the maximum complex elevation by mixing It was confirmed that the effect appeared.

When licorice and yellow dandelion extract is mixed in a weight ratio outside the above range, the composite synergy of the two extracts is reduced to show the optimal activity (Figs. 1 and 2), so the two extracts are mixed within the weight ratio range. It is desirable to.

In addition, as shown in FIG. 3, it was confirmed that iNOS expression was significantly suppressed in the mixture B and C administration groups among the experimental groups to which the mixtures A, B and C were administered, in particular, the licorice extract and the yellow dandelion extract had a weight ratio of 1: 4. It was confirmed that the maximum iNOS expression inhibitory effect in the mixture C group mixed with.

Therefore, it can be seen that the mixture of licorice extract and yellow dandelion extract in a weight ratio of 1: 1 to 1: 4 remarkably alleviates the inflammation of alcohol-induced gastric tissues and has excellent efficacy in treating alcoholic gastritis.

In the present invention, "alcoholic gastrointestinal disease" refers to any disease caused by gastric injury by alcohol. For example, it may be one or more selected from the group consisting of alcohol-induced acute gastric mucosal injury, gastric ulcer, acute gastritis and chronic gastritis, but is not limited thereto.

In addition, the mixture of licorice extract and yellow dandelion extract of the present invention may exhibit a protective effect against alcoholic liver damage.

In order to distinguish the various diseases related to the liver, it is necessary to interpret several chemical tests comprehensively. For this purpose, several test items are collectively referred to as liver function tests. Major tests include GOT, GPT, etc. In addition, many tests are performed by adding items such as total protein, albumin, and ammonia.

GOT (glutamic oxaloacetic transaminase) is an enzyme that catalyzes the reversible reaction of oxaloacetic acid and L-glutamic acid by transferring the amino group of L-aspartic acid to α-ketoglutaric acid. Measurements of this enzyme activity in the blood are frequently used in the diagnosis of hepatitis and myocardial infarction.

GPT (glutamic-pyruvic transaminase, alanine aminotransferase) is an enzyme that catalyzes the production of pyruvic acid and glutamic acid by transferring the amino group of alanine to α-ketoglutaric acid. The activity of this enzyme in the blood is often measured for the diagnosis of liver and biliary tract diseases.

Therefore, in the present invention, the improvement effect on alcoholic liver damage was confirmed by measuring GOT and GPT levels. As shown in Figure 4, the composition containing the mixture of licorice extract and yellow dandelion extract of the present invention was found to significantly reduce the GOT and GPT levels increased by the administration of alcohol, preferably ethanol. The significant synergistic effect of the mixture administration group is believed to be due to the fact that the administered mixture is absorbed faster than ethanol to reduce GOT and GPT levels.

Through the above results, it can be seen that the mixture of licorice extract and yellow dandelion extract of the present invention not only improves gastric damage by alcohol but also prevents liver damage, thereby protecting stomach and liver from excessive intake of alcohol at the same time.

The pharmaceutical compositions of the present invention may further comprise suitable carriers, excipients and diluents commonly used in the manufacture of pharmaceutical compositions.

The pharmaceutical compositions according to the present invention may be formulated in the form of powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols and the like, oral preparations, suppositories, and sterile injectable solutions, respectively, according to conventional methods. Can be used.

Carriers, excipients and diluents that may be included in the compositions of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia rubber, alginate, gelatin , Calcium phosphate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate and mineral oil.

When formulated, diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrating agents, and surfactants are usually used.

Solid form preparations for oral administration include tablets, pills, powders, granules, capsules, and the like, which form at least one excipient such as starch, calcium carbonate, sucrose or lactose in the compound. Mixed with gelatin. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Oral liquid preparations include suspensions, solvents, emulsions, and syrups, and may include various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to commonly used simple diluents such as water and liquid paraffin. .

Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solvents, suspensions, emulsions, lyophilized preparations, suppositories. As the non-aqueous solvent and suspending agent, propylene glycol, polyethylene glycol, vegetable oil such as olive oil, injectable ester such as ethyl oleate and the like can be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin butter, glycerogelatin and the like can be used.

The pharmaceutical composition of the present invention is administered in a pharmaceutically effective amount.

As used herein, the term "pharmaceutically effective amount" refers to an amount sufficient to treat a disease at a reasonable benefit / risk ratio applicable to medical treatment, and the effective dose level refers to the individual type and severity, age, sex, type of virus infected. , The activity of the drug, the sensitivity to the drug, the time of administration, the route of administration and the rate of release, the duration of treatment, factors including the concurrent drug and other factors well known in the medical field. The compositions of the present invention may be administered as individual therapeutic agents or in combination with other therapeutic agents and may be administered sequentially or simultaneously with conventional therapeutic agents. And single or multiple administrations. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect in a minimum amount without side effects, and can be easily determined by those skilled in the art.

The pharmaceutical compositions of the present invention may be used alone or in combination with methods using surgery, hormone therapy, drug therapy and biological response modifiers.

The composition can be administered to mammals such as mice, mice, livestock, humans, and the like by various routes. All modes of administration can be expected, for example, by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or cerebrovascular injections.

When the mixture of licorice extract and yellow dandelion extract of the present invention is used as a food additive, the mixture can be added as it is or used with other food or food ingredients, and can be appropriately used according to a conventional method. The mixed amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, in the manufacture of food or beverages, the composition of the present invention is added in an amount of 0.01 to 10% by weight, preferably 0.05 to 1% by weight based on the raw materials. However, in the case of prolonged ingestion for health and hygiene purposes or for health control purposes, the amount may be used below the above range.

The health functional food includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof, Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated drinks and the like. It may also contain natural fruit juices and pulp for the production of fruit juices and vegetable drinks. These components can be used independently or in combination. The dietary supplement may be any one of meat, sausage, bread, chocolate, candy, snacks, confectionary, pizza, ramen, gum, ice cream, soup, beverage, tea, functional water, drink, alcohol and vitamin complex. Can be.

The health beverage composition of the present invention is not particularly limited in liquid components except for containing a mixture of licorice extract and yellow dandelion extract, and may contain various flavors or natural carbohydrates as additional ingredients, such as ordinary drinks.

In addition, the health functional food may further include food additives, and the suitability as a "food additive" to the item in accordance with the General Regulations of the Food Additives Code and General Test Act, etc. approved by the Food and Drug Administration unless otherwise specified. Determined by the relevant standards and standards.

Items listed in the "Food Additives Code" include, for example, chemical synthetic products such as ketones, glycine, potassium citrate, nicotinic acid, cinnamic acid, natural additives such as color pigments, licorice extract, crystalline cellulose, high-quench pigments, guar gum, L Mixed preparations, such as a sodium glutamate preparation, a noodles addition alkali preparation, a preservative preparation, and a tar pigment preparation, etc. are mentioned.

Although the present invention will be described in more detail with reference to the following examples, the following examples are not intended to limit the scope of the present invention, which will be construed as to aid the understanding of the present invention.

1-1. Preparation of Licorice Root Extract and Yellow Dandelion Ground Extract

The roots of the harvested licorice and the tops of yellow dandelions were washed with water and lyophilized with a lyophilizer (Martin Christ, Osterode, Germany). The lyophilized samples were milled, and 1,000 ml of water was added to 150 g of each sample, followed by repeated extraction of hot water three times for three hours. The extract was removed by using a Whatman 46 cm filter paper, insoluble matters, and the filtered extract was concentrated under reduced pressure at 60 ℃ with a Heidolph, Germanay. In order to completely remove the solvent of the extract under reduced pressure, 50 ml of purified water was added and suspended, and then 27.5 g of licorice root extract lyophilisate and 61.3 g of yellow dandelion ground extract lyophilized were obtained using a freeze dryer (Licorice extract yield: 18.3%). , Yellow dandelion extract yield: 40.9%).

1-2. Experimental animals

Seven-week-old Spraque-Dawley male rats were purchased from Orient Bio. The light / dark cycle was set at 12 ° C at 12 hour intervals, and the diet and water were freely adapted to the basic diet for 1 week.

Confirmation of alcoholic gastritis improvement effect

2-1. Experiment set and sample administration

Each of the experimental animals of Example 1-2, 5 mice in each group, the normal group untreated, 5.0 g / kg control group administered only, 5.0 g / kg of rebamipide in use as a gastritis treatment The experiment was conducted by dividing into a positive control group dissolved in ethanol and five test groups administered each test sample dissolved in 5.0 g / kg of ethanol. Information of the test samples administered for the five test groups is as described in Table 1 below.

After the animal was fasted for 12 hours, the test sample was dissolved in ethanol (5.0 g / kg) and orally administered once, and after 1 hour, ether anesthesia was used to remove heart blood and stomach tissues after laparotomy.

Experimental group Experimental Sample Licorice root extract Yellow dandelion
Ground extract Licorice group 200 mg / kg - Yellow dandelion group - 200 mg / kg Mixture A administration group 160mg / kg 40mg / kg Mixture B administration group 100mg / kg 100mg / kg Mixture C administration group 40mg / kg 160mg / kg

2-2. Histopathological evaluation and inflammation area analysis of gastric tissue

The stomach tissues extracted from each group were fixed to observe the extent of damage and extent of damage. As a result, as shown in Figure 1, the control group administered only with ethanol, inflammation, bleeding, redness and edema of the gastric tissue occurred and the damage area was also very large. This gastric tissue injury was remarkably recovered in the mixture B and C administration groups.

As shown in FIG. 2, the area of inflammation occurrence of the licorice group and the yellow dandelion group was 43.3% and 31.0%, respectively, and the mixture A administration group, the mixture B administration group, and Inflammation area of the mixture C administration group was 41.9% and 29.6% and 20.2%.

As a result, the synergistic effect was maximized in the mixture C group in which the licorice root extract and the yellow dandelion ground extract were mixed at a weight ratio of 1: 4, compared to other experimental groups, and it was confirmed that gastric tissue damage was remarkably alleviated.

2-3. Confirmation of Inhibitory Effect of Inflammation-Related Proteins

In order to evaluate the change in the expression of inflammation-related protein (iNOS) by the administration of a mixture of licorice root extract and yellow dandelion ground extract, it was confirmed by measuring the amount of protein by Western blot test method.

After 10 μg of protein was electrophoretically separated using SDS-polyacrylamide gel, the acrylamide gel was transferred to nitrocellulose membrane. Then, it was immersed in TBS-T (0.5% Tween 20 dissolved in TBS) containing 5% skim milk and treated for 1 hour, and washed 5 times with TBS-T every 5 minutes. The prepared membrane was treated with a primary antibody and placed at 4 ° C. overnight, and then washed 5 times with TBS-T every 10 minutes. After reacting for 1 hour and 30 minutes at room temperature using a secondary antibody (diluted 1: 5000 with TBS-T) suitable for the treated primary antibody, it was washed 5 times every 10 minutes with TBS-T. After reacting with an ECL solution, photosensitized the Davinch-ChemiTM Chemiluminescence Imaging system (Davinch-K Co., Ltd., Seoul, Korea) to confirm protein expression, and the band was then replaced by the ATTO Densitograph Software (ATTO Corporation, Tokyo, Japan) program. Quantification using

As a result, as shown in FIG. 3, it was confirmed that iNOS expression was significantly suppressed in the mixtures B and C administration group of the experimental group, especially in the mixture C group in which licorice root extract and yellow dandelion ground extract were mixed in a weight ratio of 1: 4. It was confirmed that the iNOS expression inhibitory effect appeared to the maximum.

The results confirm that the licorice root extract and the yellow dandelion extract above ground extract in a weight ratio of 1: 1 to 1: 4 is very useful for the treatment of alcoholic gastritis.

Determination of liver function marker enzyme activity in serum

GOT (glutamic oxaloacetic transaminase) and GPT (glutamic-pyruvic transaminase) activity in the blood collected from the experimental animals of Example 2-1 according to the manufacturer's instructions diagnostic kit (Procedure No. 505, Sigma Chemical Co., St Louis , MO) was measured colorimetrically.

As a result, as shown in FIG. 4, the GOT and GPT levels were increased by ethanol administration (control group), and the mixture A, B, and C groups showed higher GOT and GPT levels than the licorice and yellow dandelions alone. It was found to be significantly reduced. Significant synergistic effect of the mixture administration group appears to be because the mixture is absorbed faster than ethanol to reduce GOT and GPT levels, through which the mixture of licorice root extract and yellow dandelion ground extract of the present invention is damaged by alcohol It can be seen that can effectively prevent.

Preparation Example 1 Preparation of Pharmaceutical Formulation

1-1. Manufacture of powder

A mixture of 0.5 g of licorice root extract and 2.0 g of yellow dandelion ground extract or 1.25 g of licorice root extract and 1.25 g of yellow dandelion ground extract was mixed with 1 g of lactose and filled with an airtight cloth to prepare a powder.

1-2. Manufacture of tablets

A mixture of 25 mg of licorice root extract and 100 mg of yellow dandelion ground extract or a mixture of 62.5 mg of licorice root extract and 62.5 mg of yellow dandelion ground extract is mixed with 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate. Tablets were prepared by tableting according to the preparation method.

1-3. Preparation of Capsule

A mixture of 0.5 g of licorice root extract and 2.0 g of yellow dandelion ground extract or 1.25 g of licorice root extract and 1.25 g of yellow dandelion ground extract was mixed with 100 mg of corn starch, 100 mg of lactose and 2 mg of magnesium stearate. The capsule was prepared by filling the gelatin capsule according to the preparation method of the capsule.

1-4. Preparation of Injection

Dissolve a mixture of 2.5 μg / ml licorice root extract and 10 μg / ml of yellow dandelion extract or 6.25 μg / ml of licorice root extract and 6.25 μg / ml yellow dandelion ground extract in a suitable injectable sodium chloride BP, and The pH was adjusted to pH 3.5 using dilute hydrochloric acid BP, and the volume was adjusted thoroughly using sodium chloride BP (up to 1 mL) for injection and mixed well. The solution was filled into a 5 ml Type I ampoule made of clear glass, encapsulated under an upper grid of air by dissolving the glass, and sterilized by autoclaving at 120 ° C. for at least 15 minutes to prepare an injection solution.

Preparation Example 2 Preparation of Health Food

2-1. Preparation of Cooking Seasonings

A mixture for mixing licorice root extract and yellow dandelion ground extract in a weight ratio of 4: 1 to 1: 4 was added at 0.2 to 10% by weight to prepare a cooking sauce for health promotion.

2-2. tomato ketchup  And preparation of sauces

A health promotion tomato ketchup or sauce was prepared by adding 0.2 to 1.0 wt% of a mixture of licorice root extract and yellow dandelion ground extract at a weight ratio of 1: 1 to 1: 4 to tomato ketchup or sauce.

2-3. Manufacture of Flour Food

0.1 to 5.0% by weight of the mixture of licorice root extract and yellow dandelion ground extract in a weight ratio of 1: 1 to 1: 4 was added to the flour, and bread, cake, cookies, crackers and noodles were prepared using the mixture. Health promoting foods were prepared.

2-4. Manufacture of soups and gravy

A mixture of licorice root extract and yellow dandelion ground extract in a weight ratio of 4: 1 to 1: 4 was added to the soup and broth to prepare a health-producing meat product, soup of noodles and broth.

2-5. Preparation of Ground Beef

Health promotion ground beef was prepared by adding 10 wt% of a mixture of licorice root extract and yellow dandelion ground extract in a weight ratio of 1: 1 to 1: 4 to ground beef.

2-6. Manufacture of dairy products

0.1 to 1.0% by weight of the mixture of licorice root extract and yellow dandelion ground extract in a weight ratio of 1: 1 to 1: 4 was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.

2-7. Manufacture of wire

Brown rice, barley, glutinous rice, and yulmu were alphad by a known method, and then dried and roasted to prepare a powder having a particle size of 60 mesh.

Black beans, black sesame seeds, and perilla were also steamed and dried in a known manner, and then roasted to prepare a powder having a particle size of 60 mesh.

The mixture obtained by mixing licorice root extract and yellow dandelion ground extract in a weight ratio of 1: 1 to 1: 4 was concentrated under reduced pressure in a vacuum concentrator, and dried by spraying and hot air dryer. Got.

The dry powders of the grains, seeds and licorice extracts prepared above were formulated in the following ratios.

Cereals (30% brown rice, 15% barley, 20% barley),

Seeds (7% by weight perilla, 8% by weight black beans, 7% by weight black sesame seeds),

Dry powder (1% by weight) of the mixture of licorice root extract and yellow dandelion ground extract in a weight ratio of 1: 1 to 1: 4,

Ganoderma lucidum (0.5% by weight), and

Foxglove (0.5 wt%)

2-8. Preparation of Carbonated Drinks

Syrup was prepared by mixing 5-10% of sugar, 0.05-0.3% citric acid, 0.005-0.02% caramel, 0.1-1% vitamin C, and 79-94% purified water. The syrup prepared above was sterilized at 85 to 98 ° C. for 20 to 180 seconds, mixed with cooling water at a ratio of 1: 4, and then 0.5 to 0.82% of carbon dioxide was injected to give the licorice root extract and the yellow dandelion ground extract 1: A carbonated beverage containing a mixture was prepared in a weight ratio of 1 to 1: 4.

2-9. Manufacture of health drinks

Licorice root extract and yellow dandelion ground extract were found in subsidiary materials such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%) and water (75%). The mixture was mixed homogeneously in a weight ratio of to sterilize the instant and then packaged in a small packaging container such as glass bottles, plastic bottles to prepare a healthy beverage.

Claims (10)

Alcohol-induced acute, containing a mixture of Glycyrrhiza uralensis Fischer root and Taraxacum officinale and ground extracts from which the roots were removed in an active ingredient mixture in a weight ratio of greater than 1: 1 and less than 1: 4. A pharmaceutical composition for the prevention or treatment of at least one alcoholic gastrointestinal disease selected from the group consisting of gastric mucosal injury, gastric ulcer, acute gastritis and chronic gastritis. delete The method of claim 1, wherein the licorice root and yellow dandelion, the extract of the ground portion from which the root is removed is a pharmaceutical composition, characterized in that extracted with water, C ₁ ~ C ₄ alcohol or a mixed solvent thereof. delete delete Alcohol-induced acute, containing a mixture of Glycyrrhiza uralensis Fischer root and Taraxacum officinale and ground extracts from which the roots were removed in an active ingredient mixture in a weight ratio of greater than 1: 1 and less than 1: 4. Health functional food composition for the prevention or improvement of one or more alcoholic gastrointestinal diseases selected from the group consisting of gastric mucosal injury, gastric ulcer, acute gastritis and chronic gastritis. delete 7. The dietary supplement composition according to claim 6, wherein the licorice root and the extract of the ground portion from which the flowers and roots of the yellow dandelion are removed are extracted with water, C ₁ -C ₄ alcohol or a mixed solvent thereof. delete delete

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