KR20160036118A - Cosmetic composition containing plant extracts consisting of Diospyros kaki Thunb., Castanea crenata Siebold and Zucc., Terminalia chebula Retz, Ocimum basilicum L. and Hamamelis virginiana with nano emulsion process - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a nanoemulsion extract composed of a persimmon leaf, a julienne leaf, a ghazi, a basil leaf and a positional hazel as an active ingredient and having excellent sebum secretion inhibitory effect, antiallergic effect and skin soothing effect, Extracts obtained by nanoemulsion extraction of Diospyros kaki Thunb., Castanea crenata Siebold & Zucc., Terminalia chebula Retz, Ocimum basilicum L. and Hamamelis virginiana as main materials. An anti-allergy effect and a skin soothing effect, which are contained as an active ingredient.

Description

[0001] The present invention relates to a cosmetic composition containing a nano-emulsion extract of a plant complex composed of a persimmon leaf, a persimmon leaf, a persimmon leaf, a basil leaf, and a positional hazel as an active ingredient. [0002] The present invention relates to a cosmetic composition containing plant extracts comprising Diospyros kaki Thunb., Castanea crenata Siebold and Zucc. , Ocimum basilicum L. and Hamamelis virginiana with nano emulsion process}

The present invention relates to a cosmetic composition excellent in sebum secretion suppressing effect, antiallergic effect and skin soothing effect, which comprises a plant extract composed of a persimmon leaf, a julienne leaf, a Gaza leaf, a Basil leaf and a positional hazel as an active ingredient, The extracts obtained by nanoemulsion extraction of Diospyros kaki Thunb., Castanea crenata Siebold & Zucc., Terminalia chebula Retz, Ocimum basilicum L. and Hamamelis virginiana And an anti-allergy effect and a skin soothing effect.

Patent Document: Korean Application No. 10-2010-0002424

The present inventors have found a method capable of obtaining an effective ingredient at a higher yield than the above-mentioned prior art and have applied it to cosmetics. As a result, the present inventors have found that a nanoemulsion extract composed of Persimmon leaf, Julia, Gaza, Basil leaf, The present invention provides a cosmetic composition which selectively concentrates an active ingredient including an antioxidant component and selectively applies it to a cosmetic composition to exhibit a sebum secretion inhibiting effect, an anti-allergic effect and a skin soothing effect of the present invention.

The objects and advantages of the present invention will become more apparent from the following detailed description of the invention when taken in conjunction with the accompanying drawings.

In order to achieve the above object, the present invention provides a cosmetic composition comprising a complex plant extract obtained by extracting nanoemulsions of a plant complex composed of persimmon leaves, persimmon leaves, potato leaves, basil leaves, and positional hazel.

The present invention relates to a cosmetic composition comprising a nanoemulsion extract of a plant complex composed of a persimmon leaf, a liliaceae, a basil leaf, and a positional hazel within a range of 0.1 wt% to 10 wt%, more preferably 5 wt% The present invention provides a cosmetic composition excellent in sebum secretion suppressing effect, anti-allergic effect and skin soothing effect, in addition to the skin cosmetic enhancing effect possessed by the cosmetic composition of the present invention.

 The plant complex composed of the persimmon leaves, persimmon leaves, potato leaves, basil leaf, and positional washes used in the present invention is preferably subjected to an extraction step, a filtration step, and a nano emulsion step in order to be processed in the form of a nano emulsion extract. In the nano-emulsion phase, it is made to have a size of 1 to 500 nanometers, and when it is applied to the skin, it is made to penetrate and spread to the upper part of the skin dermis layer.

If the particle size is smaller than 1 nanometer, penetration of the skin occurs easily, but it is difficult to discharge to the outside due to excessive penetration of the skin. Further, when the particle size is larger than 500 nm, penetration into the skin is ineffective.

High pressures and high shear forces are used to produce plant complexes composed of persimmon leaves, persimmon leaves, Gaza, basil leaves, and position hazel in the form of nanoparticles used in the present invention. Thus, the emulsion can be made into uniform nano-sized particles. The pressure used here is 500 bar to 2,000 bar, and the flow rate is designed to be adjusted from 20 ml / min to 150 ml / min.

Surfactants can be used in the step of preparing the nanoemulsion. Examples of usable surfactants include anionic surfactants, nonionic surfactants, cationic surfactants and amphoteric surfactants. Examples of the surfactant include polyvinyl alcohol, carboxymethylcellulose, lecithin, gelatin, polyvinylpyrrolidone, low-molecular alcohols It may be used in combination with the cosurfactant.

The amount of such a surfactant may be 0.01 wt% to 20 wt%, preferably 0.5 wt% to 5 wt%.

The following Comparative Examples and Examples are described in detail, but the technical scope of the present invention is not limited to these Examples.

Preferably, the extract is obtained by treating the plant complex at a temperature of 40 to 90 ° C for 1 to 24 hours in the step of extracting a plant complex composed of a persimmon leaf, a persimmon leaf, a goat, a basil leaf and a positional washer.

Preferably, the extraction is carried out using a solvent selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, And then extracting the mixture by using the cosmetic composition.

Preferably, the cosmetic composition is characterized by exhibiting sebum secretion inhibiting effect, anti-allergy effect and skin soothing effect.

The cosmetic composition according to the present invention contains an effective ingredient of a nanoemulsion extract of a plant complex composed of a persimmon leaf, a julienne leaf, a Gaza leaf, a Basil leaf, and a positional hazel and has no skin irritation and has solubility in various bases and additives for cosmetics, , Good affinity and stability.

The present invention provides a cosmetic composition comprising a nanoemulsion extract of a plant complex comprising a persimmon leaf, a juli, a Gaza leaf, a Basil leaf, and a positional hazel.

According to the present invention, the nanoemulsion extract of the plant complex consisting of persimmon leaves, yulpie, Gaza, basil leaves, and positional hazel was excellent in sebum secretion inhibition effect, antiallergic effect and skin soothing effect.

Therefore, the cosmetic composition of the present invention comprising the nanoemulsion extract of a plant complex composed of persimmon leaf, persimmon leaf, persimmon leaf, basil leaf, and positional hazel with the sebum secretion inhibiting effect, antiallergic effect and skin soothing effect, . ≪ / RTI >

1 is an experimental graph showing skin soothing effects according to the concentration of a nanoemulsion extract of a plant complex composed of persimmon leaves,
2 is a graph showing an anti-allergy effect according to the concentration of a nanoemulsion extract of a plant complex composed of a persimmon leaf, a julienne leaf, a potato leaf, a basil leaf,

The present invention relates to a cosmetic composition excellent in sebum secretion suppressing effect, antiallergic effect and skin soothing effect containing a nanoemulsion extract of a plant complex composed of a persimmon leaf, a julienne leaf, a goat, a basil leaf and a positional hazel, An antiallergic effect and a skin soothing effect containing an active ingredient produced by applying a nanoemulsion extraction technology to a plant complex composed of a plant complex composed of papaya, lily, basil leaf and position hazel.

As described above, the nano-emulsion extract of the plant complex comprising the persimmon leaves, the juli, the Gaza, the basil leaves and the positional hazel of the present invention is excellent in the sebum secretion suppressing effect, the anti-allergic effect and the skin soothing effect and is suitable for use as a raw material for the cosmetic composition .

The inventors of the present invention have found that a physiologically active substance having various effects can be obtained in a maximally high yield through a filtration process, The present inventors completed the present invention by confirming that the content of the active ingredient is increased by applying the nanoemulsion extraction process to the plant complex and that it is applied to cosmetics to inhibit sebum secretion, antiallergic effect and skin soothing effect.

The plant complex comprising the persimmon leaves, the persimmon leaves, the potato leaves, the basil leaves and the positional hazel in the cosmetic composition of the present invention is contained in an amount of 1 to 50% by weight on the dry weight basis, 1 to 50% by weight of the persimmon leaves, 1 to 50% 1 to 50% by weight of basil leaves and 1 to 50% by weight of positional hazel.

The persimmon leaves, persimmon leaves, ghazi, basil leaves, and positional hazelnut used in the present invention are thoroughly washed, dried and pulverized, and then finely ground to a size of 300 mesh and used in the extraction process.

It is also preferable that the extraction step is carried out in the extraction step with an extraction selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol, hexane, More preferably, ethanol, 70% ethanol aqueous solution or water is used as an extraction solvent, and most preferably, 70% ethanol aqueous solution is used for extraction.

Ethanol was added to the resulting plant extract to make a final 70% (v / v) ethanol aqueous solution, and the mixture was refluxed for 3 hours for 5 hours. After cooling, Filter with Whatman # 2 filter paper.

Upon completion of the filtration, the plant extract consisting of the persimmon leaves, the yulpie, the ghazi, the basil leaves, and the positional washer of the present invention is converted into uniform nano-sized particles using high pressure and high shear force to produce a nano emulsion.

Surfactants can be used to prepare nanoemulsions. Examples of usable surfactants include anionic surfactants, nonionic surfactants, cationic surfactants and amphoteric surfactants. Examples of the surfactant include polyvinyl alcohol, carboxymethylcellulose, lecithin, gelatin, polyvinylpyrrolidone, low-molecular alcohols It may be used in combination with the cosurfactant.

The amount of such a surfactant may be 0.01 wt% to 20 wt%, preferably 0.5 wt% to 5 wt%.

The ingredients contained in the cosmetic composition of the present invention include, in addition to the above-mentioned effective ingredients, the ingredients conventionally used in cosmetic compositions, such as antioxidants, stabilizers, solubilizers, vitamins, And a carrier.

The cosmetic composition of the present invention may be prepared in any form conventionally produced in the art and may be in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, Oils, powder foundations, emulsion foundations, wax foundations and sprays, but are not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

In the case where the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. Especially, in the case of a spray, a mixture of chlorofluorohydrocarbons, propane / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component may include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide ether Alkylamido betaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

The cosmetic composition of the present invention can be applied to cosmetics which are expected to have sebum secretion inhibiting effect, anti-allergy effect and skin soothing effect.

The cosmetic process of the present invention refers to all cosmetic processes using the cosmetic composition of the present invention. That is, all methods known in the art using a cosmetic composition belong to the cosmetic method of the present invention.

In addition, the present invention provides a cosmetic composition comprising the nanoemulsion extract consisting of the persimmon leaves, persimmon leaves, potato leaves, basil leaves, and positional hazel.

Refers to all cosmetic methods for applying the cosmetic composition of the present invention to human skin. That is, all methods known in the art for applying the cosmetic composition to the skin belong to the cosmetic method of the present invention.

The cosmetic composition of the present invention may be used alone or in combination, or may be used by overlapping with other cosmetic compositions other than the present invention. Also, the cosmetic composition having an anti-sebum secretion inhibiting effect, an anti-allergy effect and a skin soothing effect according to the present invention can be used according to a conventional method of use, and the use frequency of the cosmetic composition can be varied according to a user's skin condition or taste.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention, and it is to be understood by those skilled in the art that the present invention is not limited thereto It will be obvious.

Example 1 Preparation of Nano-emulsion Extract comprising Persimmon Leaf, Juli, Gaza, Basil Leaf and Location Hazel According to the Present Invention

20 g, 20 g, 20 g, and 20 g on the dry weight basis, respectively. The pulverized product was finely pulverized so as to pass through a 300 mesh sieve, and the final 70% (V / V) ethanol solution, and the mixture was refluxed three times for 5 hours, cooled, and filtered through Whatman # 2 filter paper. Upon completion of the filtration, in the present invention, 1 g of the extract was prepared by concentrating the extracted filtrate obtained in the extracting step and applying the nano emulsion step again.

Example 2 Preparation of Nutritive Cream Containing Nano Emulsion Extract Composed of Persimmon Leaf, Juli, Gaza, Basil Leaf and Location Hazel According to the Present Invention

A step of heating the purified water portion to 80 DEG C to dissolve, a step of emulsifying by adding an oily portion heated and dissolved at 80 DEG C, a step of adding and mixing a thickener dissolved by heating at 60 DEG C, and a step of adding and mixing cosmetic ingredients And cooled to 30 ° C., degassed and filtered, and aged at 50 ° C. to produce nutritional cream as shown in Table 1.

Classification Raw material name Weight (% by weight) Purified water portion Purified water 60.36 Propylene glycol 5.0 glycerin 6.0 Xanthan gum 0.1 Triethanolamine 0.7 Methylparaben 0.2 Phenoxyethanol 0.1 The nanoemulsion extract of the present invention 2.0 Oil component Squalane 8.0 Cetyl alcohol 2.2 Cyclopentasiloxane 2.0 Stearic acid 1.5 Glyceryl monostearate 1.0 Microcrystalline wax 0.6 PIG-100 0.3 Tocopheryl acetate 1.0 Shi Butter 1.0 Propylparaben 0.1 Orizanol 0.1 Non-sabo roll 0.2 Dimethicone 0.5 Polyoxyethylene sorbitan monostearate 0.8 Thickener Carbomer 0.24 Purified water 2.4 Beauty ingredient Sodium ascorbate 0.1 Phospholipids 1.0 Prascenta extract 0.5 Aloe extract 0.5 Tocopherol acetate 0.5 Aloe extract 1.0 Sum 100.00

Experimental Example 1 Skin irritation and protective effect of nutritional cream containing nano emulsion extract composed of persimmon leaves, yulpie, Gaza, basil leaves and positional washes according to the present invention

A predetermined amount (10 m / 5 cm ² ) of the nutritional cream prepared in Example 3 was applied to the forehead of 40 healthy adults (20 men and 20 women), and the other portions were covered with aluminum foil for 30 minutes Thereafter, a long wavelength ultraviolet ray was irradiated with a dose of 10.2 J / cm < 2 > using an ultraviolet light irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, it was confirmed that the nutritional cream had no phototoxicity and had a skin protection effect because no adverse effects such as erythema were generated in the skin. In addition, 40 healthy adults (20 males and 20 females) were divided into two groups, and the experimental group in which the nutritional cream prepared in Example 2 was applied by a predetermined amount (10 m / 5 cm ² ) , GAZA, Basil leaf, and positional Haegel, and after 1 hour, 3 hours, and 6 hours, the skin elasticity was measured as a control, and the skin elasticity was about 5N Of the present invention, it was found that the nano-emulsion extract composed of the persimmon leaves, the persimmon leaves, the basil leaf, and the positional washer It was confirmed that the skin elasticity was improved by using a nutritive cream.

<Experimental Example 2> Test for skin irritation of a nutritional cream containing a nano emulsion extract consisting of persimmon leaves, persimmon leaves, ghazi, basil leaf, and positional washes according to the present invention

The human skin test of the nutritional cream prepared in Example 2 was performed on the forehead portion of 40 healthy adult men (20 men and 20 women). The entire portion of the test subject was thoroughly wiped with 70% ethanol, and then the pin chamber containing 20 占 퐇 of the nutritional cream prepared in Example 2 was closed on the common portion for 24 hours by using an adhesive tape. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with the gauze was gently wiped off and the skin condition was observed and again after 24 hours. As a result of the experiment, in the human skin test for confirming the skin irritation of the nutrition cream containing the nano emulsion extract composed of the persimmon leaves, the yulpie, the goat, the basil leaf and the positional washer according to the present invention, It was confirmed that there is no.

<Experimental Example 3> Experiments showing inhibition of sebum secretion according to the concentration of the nano emulsion extract composed of the persimmon leaves, juli, Gaza, basil leaves and positional washer of the present invention

In order to measure the oily skin mitigation effect, 20 women with oily skin of 20 to 45 years old were applied to the forehead part as a control group and 10 as a control group, , 2 weeks, 3 weeks and 4 weeks later, sebum secretion amount of skin was measured using a skin moisture analyzer (Sebumeter SM810). The test was carried out at 20 ° C and 20% relative humidity, and the measurement results are shown in Table 2. The results were expressed as the average value of 10 subjects. In the case of oily skin, the measurement value was 220 or more and in normal skin, it was in the range of 100 to 220.

Test substance Before application 1 week 2 weeks 3 weeks 4 weeks Control group 240 238 238 237 235 Experimental group 243 239 230 215 210

As shown in the results of Table 2, the effect of suppressing sebum secretion was shown, and it was confirmed that sebum control effect was obtained. It is possible to prevent acne and trouble due to excessive sebum.

<Experimental Example 4> Experiments showing the skin soothing effect according to the concentration of the nano emulsion extract composed of the persimmon leaves, the yulpie, the goat, the basil leaf and the positional washer according to the present invention

In the same manner as in Experimental Example 3, a control sample of the nanoemulsion extract consisting of persimmon leaves, yulpie, Gaza, basil leaves, and positional washes and samples of the experimental group were used.

Dermal fibroblasts (HDFn) were suspended in DMEM (10% serum supplemented, containing 1% antibiotic) medium, cultured in a 5% CO ₂ incubator at 37 ° C, and grown in the log phase. Wells were inoculated in 96 wells at a rate of about 1 x 10 ⁵ cells per well. The sample extracts were then treated with various doses and incubated for 20 min at 37 ° C in a 5% CO ₂ incubator. Then, 1 mM of H ₂ O _{2 as an} oxidative stress inducer was added, and 20 μM of DCFH-DA (stock 20 mM) was added thereto, and the spectrum was measured with a spectrofluorophotometer (excitation 485 nm, emission 535 nm). The reaction was observed at 37 DEG C for 90 minutes. The inhibition rate of reactive oxygen species was determined by comparing the number of reactive oxygen species formation with the number of active oxygen species (control group), and comparing with the group treated with a sample to remove active oxygen species. All experiments were repeated three times Respectively. As a result, the inhibition rate of 24% active oxygen species was shown at a concentration of 100 μg / ml.

FIG. 1 shows that the skin soothing effect is increased as the concentration of the nanoemulsion extract composed of the persimmon leaves, yulpie, gauze, basil leaf, and positional washer according to the present invention is increased.

(%) = ((Control OD - sample treated OD) / control OD} x 100

<Experimental Example 5> Experiments showing the anti-allergy effects of the nano-emulsion extract composed of the persimmon leaves, juli, Gaza, basil leaves and positional washes according to the present invention

RBL-2H3 cells were suspended in DMEM (Dulbecco's modified Eagle's medium) containing 10% FBS and treated with 2 x 10 ⁵ cells per well in a 24-well plate. The cells were sensitized with 100 ng / mL mouse monoclonal IgE per well and cultured in a 5% CO ₂ incubator for 24 hours. Cells were rinsed with 500 μl of Tyrode buffer, treated with mixed extracts at various concentrations and incubated for 15 min at 37 ° C in a 5% CO ₂ incubator and treated with 100 ng / ml DNP-BSA for 1 h. The substrate

(1 mM p-nitrophenyl-N-acetyl-ß-D-glucosaminide), incubated at 37 ° C in a 5% CO ₂ incubator for 1 hour and absorbance was measured at 405 nm using an ELISA reader. Especially, at the concentration of 100 ug / ml, it showed 20% allergic inhibitory effect.

The results are shown in FIG. 2, and it was confirmed that the amount of released β-hexosaminidase was decreased according to the concentration of the nanoemulsion extract composed of persimmon leaf, yulpie, Gaza, basil leaf, and positional washer, .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but on the contrary, &Lt; RTI ID = 0.0 &gt; and / or &lt; / RTI &gt;

Claims (6)

A cosmetic composition containing a nano emulsion extract composed of persimmon leaves, persimmon leaves, The cosmetic composition according to claim 1, wherein the nanoemulsion extract is contained in an amount of 0.01 to 10% by weight on a dry weight basis The cosmetic composition according to claim 1, characterized in that the cosmetic composition comprising the nanoemulsion extract has a softening lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, Cosmetic composition The cosmetic composition according to claim 1, wherein the nanoemulsion extract has sebum secretion inhibiting effect
The cosmetic composition according to claim 1, wherein the nanoemulsion extract has an anti-allergic effect The cosmetic composition according to claim 1, wherein the nanoemulsion extract has a skin soothing effect

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