KR20210012536A - Cosmetic composition having Anti-pollution effect comprising Lotus leaf extracts as an effective component - Google Patents

Abstract

The present invention discloses a cosmetic composition having an anti-pollution effect containing a lotus leaf extract obtained by ultrasonication of a lotus leaf pulverized product in 10 times 1,3-butylene glycol as an active component. According to the present invention, an ultrasonic extract of 40-60% butylene glycol of lotus leaves has an excellent effect of providing a novel cosmetic composition and cosmetics of various formulations accordingly.

Description

Cosmetic composition having Anti-pollution effect comprising Lotus leaf extracts as an effective component}

The present invention relates to a composition for skin anti-pollution containing a lotus leaf extract as an active ingredient and a method for producing the same.

As skin aging due to environmental pollution and fine dust increases, the cosmetic industry has been developing products with an'anti-pollution' function since 2005. A general anti-pollution product has been proposed to remove heavy metals and fine dust penetrating into cell pores through cleansing, but it is difficult to effectively remove heavy metals and fine dust with strong adsorption power only with simple cleansing. In addition, since products with strong cleansing power contain chemical substances that are highly irritating to the skin, various skin diseases may occur in the case of sensitive or sensitive skin.

Accordingly, the development of anti-pollution products uses natural products to reduce skin irritation caused by various chemical substances. Bioactive substances obtained from natural products not only maintain skin health by maintaining the skin's own function and activating skin cells, but also have high stability. Recently, as green tea-fermented extracts have been found to be effective in alleviating skin damage caused by fine dust, the development of cosmetics fermented with natural products is increasing.

Therefore, in a situation where the need for the development of cosmetic products derived from natural products having excellent skin protection effects against environmental stress such as fine dust in the air is constantly emerging, the present inventors set out to develop a functional cosmetic composition excellent with natural extracts.

Lotus leaf (Nelumbo nucifera) is a dicotyledonous plant belonging to side leaf plants among aquatic plants, and is widely distributed in Korea, China, and Japan, including tropical and temperate eastern Asia, mainly in India and China. (Borsch, 1983). Lotus leaves have a bitter taste and good properties, and are known to stabilize the body and mind, exhausted in the summer, to increase appetite. In addition, it has been used as a folk remedy for hemorrhagic gastric ulcer, gastritis, hemorrhoids, hemostasis, diarrhea, headache and anemia, postpartum blood stagnation, nocturia, and detoxification (Kim, 1981). The ingredients of lotus leaf contain analgesic and soothing properties such as roemerine, nuciferin, armepavine, n-nornuciferine, pronuciferine, liriodenine, d-n-methylcoclaurine, tartaric acid, citric acid, succinic acid, and tannin (Yuk, 1990). In addition, calcium and potassium content is relatively high, and antioxidant vitamins such as β-carotene and vitamins C and E remove free radicals, which prevents coronary artery disease, cancer, and cataracts, and is also effective in anti-aging. In particular, flavonoids in green leaves increase the activity of antioxidant enzymes as well as superoxide dismutase (SOD), glutathione peroxide, scavenging of catalase and peroxyl nitrate, chelating of Fe and Cu, and thus lipid peroxidation and low density lipoprotein (LDL). Prevents oxidation (Kyung-Seok Lee, 2006; Jin-ah Im, 2008). In addition, due to the polyphenols contained in lotus leaves, antioxidants (Kum-suk Kim, 2008; Chanheum Park, 2007; LIN et al., 2009), antibacterial (Cheonhyun Kim, 2008; Yunjeom Park, 2008; Mingyu et al., 2008), etc. It has a physiological activity and is known to be good for women's diseases.

As described above, studies on lotus leaves have been conducted, but no studies have been conducted on lotus leaf extracts and anti-pollution cosmetic compositions containing the same as an active ingredient. Accordingly, the present inventors reached the present invention by conducting a study using lotus leaf extract.

Recently, an anti-pollution cosmetic for preventing skin aging due to environmental pollution and fine dust is extracted from the pulverized product of the Areca palm (Chrysalidorcarpus lutescens) plant with ethanol, filtered through a membrane filter, and the pulverized product of the Areca palm plant. A cosmetic composition for anti-inflammatory skin moisturization and skin protection using ethanol extract cultured by inoculating flower mushroom mycelium has been proposed in Korean Patent No. 10-1825713.

In addition, hot water extracts at 100 to 120°C of excellent anti-wrinkle lotus, lotus leaf, lotus stem, lotus root, and lotus meat according to the elastase inhibition effect and collagen synthesis effect are disclosed in Korean Patent Publication No. 10 -It has been disclosed in 2011-0115047.

On the other hand, the antibacterial, antioxidant and anti-inflammatory effects of the ethanol extract of lotus leaf against three dermatophytes were disclosed (Reserch Article, kor. J. Aesthet. cosmetol., vol. 10 No. 2, 405-413 , 2012.5).

However, so far, there has not been a study on the skin protection effect by the whitening activity using lotus leaf extract and the cell survival effect by particulate matter (PM). The present inventors have completed the present invention for new discoveries and have filed this patent application.

Accordingly, an object of the present invention is to provide a lotus leaf extract and a cosmetic composition containing the same as an active ingredient.

Another object of the present invention is to provide a method for preparing the lotus leaf extract and a cosmetic composition for whitening and skin protection comprising the lotus leaf extract prepared by the method as an active ingredient.

The object of the present invention is to wash and dry lotus leaves with water;

Grinding the washed and dried lotus leaves obtained in the above step;

Adding the pulverized lotus leaf obtained in the above step to 40-60% butylene glycol (Butylene Glycol), fixing it at a temperature of 40-50° C. using a sonicator (JAC. 4020, Jinwoo, Korea) and extracting; The filtrate obtained by filtering the sample extracted in the above step with a 0.45 µm membrane filter was achieved by evaluating the antioxidant activity effect, whitening activity effect, and skin protection activity effect related to environmental stress.

According to the present invention, the ultrasonic extract of 40-60% butylene glycol of lotus leaves has excellent antioxidant activity, whitening activity, as well as skin protection activity, and thus has excellent effects that can provide novel cosmetic compositions and cosmetics of various formulations. have.

1 is a graph verifying the SOD-like activity effect of the ultrasonic extract of 1,3-butylene glycol according to the present invention,
2 is a graph verifying the DPPH radical scavenging ability,
Figure 3 is a graph verifying the ROS production inhibitory activity
4 is a graph showing the experimental results of the skin whitening activity effect of the ultrasonic extract of 1,3-butylene glycol according to the present invention;
5 is a graph showing the cell viability experiment results for fine dust,
6 is a graph verifying the ability to inhibit apoptosis (apoptosis).

Hereinafter, the present invention will be described in more detail through examples according to the present application. However, the following examples of the present application are in spite of one example of the present application. Therefore, these examples are only for describing the present invention in more detail, and that the scope of the present invention described in the appended claims is not limited by these examples, for those skilled in the art to which the present invention pertains. It is obvious.

According to an embodiment of the present invention, a cosmetic composition comprising the lotus leaf extract as an active ingredient is provided.

The cosmetic composition refers to a functional composition having excellent antioxidant, skin whitening activity and skin protection effects.

The skin protection effect includes protecting the human skin from environmental stress caused by skin damage such as fine dust, yellow dust, cigarette smoke, and soot.

Herein, the term "active ingredient" means containing a content of a minimum range value or more that can express the above-mentioned effect.

"Lotus leaf extract" of the present invention means an ultrasonic extract and includes water (H ₂ O) or an organic solvent extract containing a lower alcohol having 1 to 4 carbon atoms, especially ethanol, but most preferably 1,3-butylene glycol ( Butylene Glycol) refers to the ultrasonic extraction by contacting the pulverized lotus leaf powder after drying as a raw material in a solvent.

According to the present invention, the active ingredient contained in the lotus leaf as a raw material has a different extract depending on the polarity of the solvent, and the butylene glycol has excellent selectivity in the extraction of physiologically active substances compared to water, ethanol, or methanol, so that the solvent 1, Ultrasound extract from 3-butylene glycol is most effective in realizing skin whitening and skin protection effects.

Since water, ethanol, and 1,3-butylene glycol have polarity above and there is a difference in the active ingredients extracted according to each polarity, the concentration of 1,3-butylene glycol will maximize the optimal whitening activity and skin protection activity. The concentration must be controlled so that it can be done. If the concentration of 1,3-butylene glycol is less than 40%, the active ingredient exhibiting skin improvement effect is not sufficiently extracted, and if it is more than 60%, the extraction yield deteriorates.

The lotus leaf extract is preferably extracted for 12 to 15 hours at 40 to 50°C using the water or an organic solvent.

When the lotus leaf extract is extracted using an organic solvent, the extraction efficiency decreases when it is less than 40℃ and the active ingredient content decreases. When extracted at 50℃ or higher, the glycoprotein, an active ingredient contained in natural products, is denatured at high temperature and It was not preferable because the improvement effect was reduced. In addition, when the extraction time is less than 12 hours, the extraction of the active ingredient contained in the natural substance is incomplete, and when the extraction time exceeds 15 hours, the content of the residual active ingredient contained in the lotus leaf is insufficient, resulting in a decrease in time and cost efficiency.

According to the present invention, the amount of lotus leaf extract used is preferably from 0.005% to 20% by weight based on the total weight of the cosmetic composition. If the content of the lotus leaf extract is less than 0.005% by weight, it is difficult to realize sufficient activity of the active ingredient, and if it exceeds 20% by weight, it may interfere with the efficiency of product cost.

The cosmetic composition may improve skin protection and whitening effects against environmental stress by removing fine dust and heavy metals.

In addition to removing fine dust or heavy metals, the environmental stress reduction is all intended to prevent the accumulation of any substances that may damage the skin, such as yellow dust and soot cigarette smoke, if the accumulation is encouraged. In particular, protecting human skin means improving the dead skin cells of the skin and promoting skin activity.

The cosmetic composition includes softening lotion, nutritional lotion, moisture cream, nutrition cream, massage cream, nutrition lotion, essence, ampoule, gel, eye cream, oil, foundation, cleansing cream, cleansing foam, cleansing water, shampoo, conditioner, pack and It may be formulated with one or more selected from the group consisting of powder.

The cosmetic composition may be formulated by a conventional method. Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA can refer to the contents disclosed in the formulation of external preparations for skin, and the International cosmetic ingredient dictionary, 6th (The cosmetic, Toiletry and Fragrance Association, Inc., Washington, 1995).

Specifically, the cosmetic composition may be prepared in a general emulsion formulation and solubilization formulation. For example, a lotion such as a flexible lotion or a nutritional lotion; Emulsions such as facial-lotion and body lotion; Creams such as nutrition-cream, moisture-cream, and eye-cream; essence; Cosmetic ointment; spray; Gel; pack; Sunscreen; Makeup base; Foundations such as liquid type, solid type or spray type; powder; Makeup removers such as cleansing-cream, cleansing-lotion, and cleansing-oil; Alternatively, it may be formulated with a detergent such as cleansing foam, soap, and body wash, but is not limited thereto. In addition, the external preparation for skin may be formulated as an ointment, patch, gel, cream, or spray, but is not limited thereto.

In the cosmetic composition, in addition to the essential ingredients in each formulation, other ingredients may be appropriately blended within a range that does not impair the object according to the present invention depending on the type of formulation or purpose of use.

The cosmetic composition may contain a generally acceptable carrier, for example, oil, water, surfactant, moisturizer, lower alcohol, thickener, chelating agent, colorant, preservative, fragrance, etc. may be appropriately blended, but are limited thereto. no.

The acceptable carrier may vary depending on the formulation. For example, when formulated as an ointment, paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, or their Mixtures can be used.

When the cosmetic composition is formulated as a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder, or mixtures thereof may be used as a carrier component. It may further comprise a propellant such as carbon, propane, butane or dimethyl ether.

When the cosmetic composition is formulated as a solution or emulsion, a solvent, a solubilizing agent, or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl benzoate, propylene glycol, 1,3-Butylglycol oil may be used, and in particular cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan may be used.

When the cosmetic composition is formulated as a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like can be used.

When the cosmetic composition is formulated with soap, as a carrier component, an alkali metal salt of a fatty acid, a fatty acid hemiester salt, a fatty acid protein hydrolyzate, isethionate, a lanolin derivative, a fatty alcohol, vegetable oil, glycerol, sugar, etc. Can be used.

The cosmetic composition is a fatty substance, an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a foaming agent, and a fragrance, which are commonly used in the industry depending on the quality or function of the final product. , Surfactants, water, ionic or nonionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, commonly used in cosmetics It may additionally contain adjuvants commonly used in the field of cosmetology or dermatology, such as any other ingredients.

However, the adjuvant and its mixing ratio may be appropriately selected so as not to affect the desirable properties of the cosmetic composition according to the present invention.

It is apparent that the present invention is further elaborated through the following examples, but the present invention is not limited by the following examples.

Preparation of the present invention lotus leaf extract

The lotus leaves were washed with water, dried completely at room temperature, and pulverized with a grinder to obtain 100 g of lotus leaf powder.

For 100 g of the lotus leaf powder, 50% 1,3-butylene glycol was used as a solvent and mixed at 1:10 (w/w), and then the temperature was adjusted using an ultrasonicator (sonicator, JAC. 4020, Jinwoo, Korea). It was fixed at 45° C. and extracted for 13 hours. The extracted sample was filtered using a 0.45 μm membrane filter, and about 950 mL of the filtrate was obtained.

[Comparative Examples 1 to 3]

Conducted according to Example 1, but in Comparative Example 1, 10 times purified water was added and hot water was extracted at 100°C for 2 hours,

In Comparative Example 2, 50% ethanol was added with 10 times the solvent and extracted for 13 hours in a shaking incubator at 25°C,

Finally, in Comparative Example 3, 30% ethanol was added 10 times as a solvent and extracted for 13 hours in a shaking incubator at 25°C.

The yield (g) obtained as a result of solvent extraction according to Examples and Comparative Examples 1 to 3 of the present invention was different as shown in [Table 1] according to the seed oil and treatment conditions of the solvent used.

division menstruum Yield Treatment conditions Storage conditions Example 1 50% 1,3-butylene glycol 135.2 Ultrasonicator 40.5kHz/0.45㎛ filter filtration -4℃ Comparative Example 1 Hot water extraction 100℃/2 hours 72.5 Hot water extractor/
Cooling after filtering 0.45㎛ filter -4℃ Comparative Example 2 50% ethanol 82.2 Shaking incubator/
After centrifugation, evaporation and concentration under reduced pressure rotation -20℃ Comparative Example 3 30% ethanol 89.5 Shaking incubator/
After centrifugation, evaporation and concentration under reduced pressure rotation -20℃

According to Example 1, the lotus leaf extract having the best yield was obtained, and it was stored in a refrigerator at -4°C and provided in the following experiment.

[Experimental Example 1]

SOD-like active ingredient

Superoxide dismutase (SOD) is an antioxidant enzyme that converts intracellular reactive oxygen species (ROS) into H ₂ O ₂ and the generated H ₂ O ₂ is water by peroxidase or catalase. And decomposed into oxygen.

In other words, it serves to protect the living body by inhibiting the oxidation reaction of superoxide.

The measurement of the SOD-like activity of the prepared lotus leaf extract was measured by modifying the Nishikimi method. To the sample, 3 mM Xanthine, 3 mM EDTA, BSA, PPE (0.25-5.0 mg), PMS (30 mM), NADH (338 mM) and NBT (0.75 mM)) were added to the phosphate buffer calibrated to pH 7.4. After reacting at °C for 10 minutes, 1 N HCl was added to measure absorbance at 420 nm. The SOD-like activity was expressed as a percentage (%) of the difference in absorbance between the extract-added group and the control group.

A buffer solution (50 mM KH ₂ PO ₄ /KOH (pH 7.4) buffer, 15 mM Na ₂ EDTA in buffer, 3 mM Hypoxanthine in 50 mM KOH, 0.6 mM NBT in buffer) was prepared, and the mixture solution was 1 well. Sugar 15 mM Na ₂ EDTA 20 μL, 0.6 mM NBT 50 μL, 3 mM Hypoxanthine 30 μL, 50 mM KH ₂ PO ₄ /KOH 145 μL, hemp extract, hemp extract, hemp extract and hemp extract were mixed. 5 μL (put in the first row), Xanthine oxidase 50 μL (0.1 units/mL in 50 mM KH ₂ PO ₄ /KOH).

Add 245 μL of the mixed solution and 5 μL of each extract to the first row, add 125 μL of the mixture, and dilute it downward in the first row to adjust the concentration, then add 125 μL of the mixed solution to all wells to 50 μL of Xanthine oxidase. After setting the reaction rate value using a Fluorescence Multi-Detection Reader (Bio-Tek, USA) in a total volume of 300 μL, absorbance was measured at 570 nm for 5 minutes in increments of 20 seconds.

The experimental results were increased in a concentration-dependent manner as shown in FIG. 1.

[Experimental Example 2]

DPPH radical scavenging ability experiment

1,1-diphenyl-2-picrylhydrazyl (DPPH, Sigma, USA) radical scavenging activity was measured by the method of Blois (Blois, MS “Antioxidant determination by the use of a stable free radical”, Nature 181:1199-1200, 1958). I did. The sample was dissolved in MeOH, 500 μL was added to a 1.5 mL tube, and 500 μL 0.3 mM DPPH was added so that the total amount was 1 mL. After reacting at room temperature for 30 minutes, absorbance was measured at a wavelength of 517 nm with an ELISA reader (Bio-Tek, USA). DPPH radical scavenging ability was expressed as a percentage of the difference in absorbance between the addition and no addition of the sample solution. DPPH radical scavenging ability = 100-{(absorbance with added sample/absorbance without added sample)×100}.

The experimental results are shown in FIG. 2.

[Experimental Example 3]

ROS production inhibitory efficacy of the lotus leaf extract of the present invention

In order to test the antioxidant activity of the extract according to the present invention, DCFH-DA (2'7'-dichloro flurescin diacetate) was used. For this purpose, skin fibroblasts (HDFn) were suspended in DMEM (10% serum and 1% antibiotics added) medium and cultured for 24 hours in a 37°C, 5% CO ₂ incubator to a number of 1×10 ⁵ cells per well. The skin fibroblasts obtained in the above step were treated by extract concentration and incubated for 1 hour in an incubator at 37°C and 5% CO ₂ , and then 1 mM of H ₂ O ₂ and DCFH-DA 20 M, which are oxidative stress inducing substances, were added. It was measured with a spectrofluorophotometer under the conditions of 485 nm and emission 535 nm. The treatment group whose antioxidant activity was measured without adding a sample was used as a control, and the antioxidant activity was calculated by the method of Equation 1 below. All experiments were repeated three times.

Experimental results showed 25% photoaging inhibitory effect as shown in FIG. 3.

[Experimental Example 4]

Whitening activity test of the lotus leaf extract of the present invention

Melanin formation inhibition experiment

Murine melanoma (B16 F1) cells were inoculated into a 24-well plate in DMEM medium containing an appropriate amount of FBS (fetal bovine serum) at 2×0 ⁴ per well, and then cultured at 5% CO ₂ and 37°C for 24 hours. After incubation, the medium was removed, and the sample was diluted to an appropriate concentration in a medium containing Alpha-Melanovyte-stimulating hormone and treated, followed by incubation at 5% CO ₂ , 37°C for a certain time. After culturing, the medium was removed, and 120 μL of cell lysis buffer was added to obtain intracellular melanin in an oven at 60°C. Using the above solution, absorbance was measured at 405 nm with a microplate measuring instrument to determine the amount of melanin per certain protein.

As a result of the experiment, when the lotus leaf extract of the present invention was treated, 39% of melanin production was inhibited (FIG. 4).

[Experimental Example 5]

Cell viability test for PM2.5 of the lotus leaf extract of the present invention

The cell viability experiment for PM (Particulate Matter, fine dust) of the lotus leaf extract according to the present invention was conducted based on the MTT reagent reaction method using HaCaT cells, human keratinocytes. HaCaT cells were inoculated into 96-well plates at 4 X 10 ⁴ cells/well. After 24 hours, the extract was treated by concentration in a medium containing 100 μg/mL of PM2.5 and cultured for 24 hours, and then, 100 μL of 5 mg/mL (PBS) MTT reagent was treated and cultured for 4 hours. After removing the medium, the remaining precipitate was dissolved in 150 μL of DMSO, and the absorbance of each sample was measured at 570 nm. The experiment was repeated 3 times, and the significance test was analyzed using SPSS19.0 software. If P<0.05 or P<0.01, it was judged to be significant.

As a result of exposure to PM2.5, the survival rate of HaCaT cells was significantly reduced to 53.93% compared to the control group. On the other hand, as a result of treating the lotus leaf extract at concentrations of 2, 4, and 8 μg/mL, the cell viability was significantly 60.02% (P<0.05), 63.05% (P<0.01), and 70.25% (P<0.001), respectively. Increased.

The experimental results are shown in FIG. 5.

[Experimental Example 6]

Apoptosis (apoptosis) inhibition test for PM2.5 of the lotus leaf extract of the present invention

The experiment of inhibiting apoptosis against PM (Particulate Matter, fine dust) of lotus leaf extract was confirmed by HaCaT cells, human keratinocytes. HaCaT cells were treated with a concentration of blank control, PM2.5 100 ug/mL, lotus leaf extract 2, 4, and 8 μ, respectively. After that, the rate of inhibition of cell death (apoptosis) was confirmed using a flow cytometer.

After inducing apoptosis by PM2.5, when treating lotus leaf extract by concentration, 13.9%±0.7% (P<0.05), 11.7%±1.3% (P<0.05), and 8.3%±1.4% (P< 0.01) was confirmed to inhibit cell death (Fig. 6).

The present invention provides a novel lotus leaf extract obtained by ultrasonic treatment of dry powdered lotus leaf powder in a 1,3-butylene glycol solvent, and has an excellent effect of providing a novel whitening and skin protection cosmetic composition using the lotus leaf extract. It is a very useful invention in the cosmetic industry.

Claims (4)

Lotus leaf extract obtained by ultrasonically treating lotus leaf powder in 10 times of 40-60% 1,3-butylene glycol solvent. An antioxidant and skin whitening cosmetic composition containing the lotus leaf extract of claim 1 as an active ingredient. A cosmetic composition for skin protection containing the lotus leaf extract of claim 1 as an active ingredient. The method according to any one of claims 2 to 3, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, milk lotion, moisturizer lotion, nutrition cream, massage cream, hand cream, foundation, essence, pack, soap, A cosmetic composition formulated with one or more selected from cleansing foam, cleansing lotion, cleansing cream, shampoo, and conditioner.

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