KR20160017880A - Cultivating method of mushroom using sawdust media - Google Patents

Abstract

The present invention provides a method for cultivating a mushroom using a sawdust medium. The method for cultivating a mushroom using a sawdust medium comprises: a step of manufacturing a sawdust medium for mushroom cultivation comprising oak tree sawdust, calcium carbonate, wheat bran, cottonseed bran, brown sugar, water, nano-detoxification sulfur, and anthocyan; a step of inserting the sawdust medium into a cultivation container, sealing the cultivation container, and then heating and cooling the cultivation container for sterilization; a step of injecting mushroom spawn into the cooled sawdust medium; a step of cultivating the mushroom spawn in the dark to cultivate a mycelium; a step of cultivating the mushroom mycelium which has undergone dark cultivation in a lit environment to allow browning; and a step of inserting the cultivation container containing the browned mushroom mycelium into a cultivation facility to grow the mushroom mycelium into a mushroom.

Description

TECHNICAL FIELD The present invention relates to a method for cultivating mushroom using a sawdust medium,

More particularly, the present invention relates to a method of cultivating mushroom using a sawdust medium, and more particularly, to a method of cultivating mushroom, And also to a method of cultivating mushroom using sawdust medium which is capable of cultivating functional mushroom enhanced in sulfur and anthocyanin and also providing a health promotion effect to the public.

In general, mushrooms are nutrient-requiring microorganisms that do not have photosynthesis but utilize organic carbon.

Mushroom belonging to the basidiomycetes has fruiting body that grows and develops by decomposing various organic materials. It is a food material which has a higher nutritional value than ordinary vegetables, and is a typical health food which is known to excel in hypertension, diabetes and anticancer effect It is one.

In recent years, as the level of living has improved and interest in the public health has increased, interest in concentrating clean agricultural products and high nutritional foods has been increasing, and efforts have been made to add the desired nutrients, elements or substances to existing agricultural products, Is continuing.

On the other hand, as a method of cultivating these mushrooms, there is known a method of growing the logs using hardwoods such as hardwoods and a method of cultivating them in facilities having certain facilities. However, the above-mentioned timber cultivation method is easy to manage and has a small facility cost. On the other hand, the recovery rate of mushroom is low compared to the amount of raw material, and it is difficult to purchase timber. Recently, Is growing rapidly in Korea.

At this time, in the aforementioned facility cultivation method, the medium produced by inoculating the seeds into the sawdust medium is usually used, and the sawdust medium itself is packed in a box to carry out single-phase cultivation. Since such a single-phase cultivation is a well- Is omitted. In addition, the cultivation method is described in detail in Korean Patent Laid-Open Publication No. 85-909 (Method for cultivating shiitake using hardwood sawdust) and Publication No. 90-7303 (Method for cultivating sawdust in an elevation).

According to the conventional single-phase cultivation method, the vitality of seed bacterium is lowered, the problem of contamination by germs and fungi is not solved, and only a uniform product which can not differentiate from conventional mushrooms can be harvested.

As the trend of rural agriculture and the aging of the labor force are intensifying, mushroom cultivation is limited in economic efficiency, and scientific and high value added mushroom cultivation is urgently needed.

DISCLOSURE Technical Problem The present invention has been conceived to solve the problems of the prior art as described above, and it is an object of the present invention to provide vigor of the seedling inoculated to the medium, to prevent contamination of the germs and to promote the growth of mushroom, The present invention provides a mushroom cultivation method using a sawdust medium which contributes to the promotion of income of a mushroom cultivator and further enables functional mushrooms enhanced in sulfur and anthocyanin to be cultivated, thereby providing a public health promotion effect.

The technical problem of the present invention as described above is achieved by the following means.

(1) preparing a sawdust medium for mushroom cultivation comprising oak sawdust, calcium carbonate, wheat bran, cotton bark, brown sugar, water, nano-desorbed sulfur and anthocyanin; The method comprising: inoculating the sawdust medium into a culture container, sealing the mixture, heating and cooling the paste medium for sterilization, inoculating the cooled sawdust medium with mushroom seeds, Culturing the mycelium by culturing the mushroom strain in an arm culture; Culturing the mushroom mycelium which has undergone the arm culturing and culturing the mushroom mycelium; And the mushroom mycelium which has undergone the change of browning is introduced into a grower and cultured in a mushroom.

(2) In the above (1)

The present invention relates to a method for producing a fermented soybean oil, comprising the steps of: preparing a soybean oil containing 40 to 50% by weight of oak sawdust, 0.1 to 1.0% by weight of calcium carbonate, 5 to 10% by weight of wheat bran, 1 to 5% To 0.1% by weight of anthocyanins and 0.01 to 0.1% by weight of anthocyanins.

(3) In the above (1)

And 10 to 20% by weight of peat moss.

(4) In the above (1)

And 1 to 5% by weight of a woodpecker. The method of cultivating mushroom using the sawdust medium.

(5) In the above (1)

And 1 to 5% by weight of a silk peptide.

(6) In the above (1)

A peach moss 10 to 20% by weight, a vinegar solution 1 to 5% by weight, and a silk peptide 1 to 5% by weight.

(7) The mushroom grown by any one of the above methods.

As described above, according to the present invention, it is possible to increase the yield of the mushroom cultivator by imparting the vigor of the seeds inoculated to the medium, preventing the contamination of various kinds of bacteria and promoting the growth of the mushroom and contributing to the increase of the income of the mushroom cultivator, This enhanced functional mushroom can be cultivated, thus providing the effect of enhancing public health.

FIG. 1 is a flow chart of mushroom cultivation using a sawdust medium according to the present invention.

The method for growing mushroom using the sawdust medium according to the present invention comprises:

Preparing a sawdust medium for mushroom cultivation comprising oak sawdust, calcium carbonate, wheat bran, cotton bark, brown sugar, water, nano-decontamination sulfur and anthocyanin; The method comprising: inoculating the sawdust medium into a culture container, sealing the mixture, heating and cooling the paste medium for sterilization, inoculating the cooled sawdust medium with mushroom seeds, Culturing the mycelium by culturing the mushroom strain in an arm culture; Culturing the mushroom mycelium which has undergone the arm culturing and culturing the mushroom mycelium; And a mushroom mycelium which has undergone the change of browning, is introduced into a grower and cultured in mushrooms.

Hereinafter, the contents of the present invention will be described in more detail with reference to the embodiment shown in FIG.

The sawdust medium for mushroom cultivation according to the present invention preferably comprises 40 to 50% by weight of oak sawdust, 0.1 to 1.0% by weight of calcium carbonate, 5 to 10% by weight of wheat, 1 to 5% by weight of cottonseed, 0.1 to 1.0% 30 to 50% by weight of water, 0.01 to 0.1% by weight of nano-adsorbed sulfur and 0.01 to 0.1% by weight of anthocyanin.

When the content of each component is below the lower limit value, it is difficult to reduce the yield of the mushroom or to provide the desired physiological activity, and the content of each component If the upper limit of the range is exceeded, there is a risk that the yield will be lowered.

Accordingly, the preferred embodiment of the present invention is characterized by comprising 40 to 50 wt% of oak sawdust, 0.1 to 1.0 wt% of calcium carbonate, 5 to 10 wt% of wheat pest, 1 to 5 wt% of cottonseed, 0.1 to 1.0 wt% of brown sugar, 30 to 50% by weight of the mushroom is combined with the mushroom to satisfy the optimal conditions for both the enhancement of the vitality and the increase of the yield of the mushroom.

In the present invention, especially by adding the nano-adsorbed sulfur to the sawdust medium, the sulfur component can be transferred to the inside of the mushroom, thereby improving the anticancer activity, various skin diseases, restoring tack, Thrombolysis, bone strengthening, inflammation removal and sterilization, diuretic and constipation-inhibiting action, and diabetic disease.

It is preferable to add 0.01 to 0.1% by weight of nano-adsorbed sulfur. If the amount is less than 0.01% by weight, the transition rate of sulfur is significantly lowered. If the content is more than 0.1% by weight, the growth of mushroom is inhibited, There is a possibility of kneading, and it is added in the above range.

In the present invention, anthocyanin is added to the sawdust medium. Anthocyanin is a water-soluble pigment appearing on flower, fruit and leaf of a plant, and is added to sawdust medium to allow anthocyanin to migrate into the mushroom. Therefore, when the consumer consumes the mushroom contained in the anthocyanin, the antioxidant activity and the anticancer activity are additionally provided in addition to the physiological activity effect provided by the sulfur.

Anthocyanin is preferably added in an amount of from 0.01 to 0.1% by weight, and if it is added in an amount of less than 0.01% by weight, the migration rate of anthocyanin is significantly lowered. If the content of the anthocyanin exceeds 0.1% by weight, And added in the above range.

The sawdust medium according to the present invention may further comprise at least one or more components selected from the group consisting of peat moss, vinegary and silk peptides. The content of the peat moss, vinegar solution and silk peptide is not particularly limited, but it is preferably 10 to 20 wt% peat moss, 1 to 5 wt% of wood vinegar, and 1 to 5 wt% of silk peptide.

By adding the peat moss, vinegar solution and silk peptide to the sawdust medium, it is possible to more completely block contamination from germs and fungi, and it is possible to sufficiently supply various minerals and nutrients necessary for the growth of mushroom, It also has the effect of increasing the yield.

As shown in FIG. 1, the method for cultivating mushroom using the sawdust medium according to the present invention is as follows. First, as shown in FIG. 1, A process for preparing the medium composition is necessary.

The sawdust medium thus prepared is placed in a culture container and bundled. The medium container is placed in a sterilizer at 90 to 110 ° C, preferably 100 ° C for 50 to 70 minutes, preferably 60 minutes, 120 to 125 ° C, After further sterilization at 121 DEG C for 80 to 100 minutes, preferably 90 minutes, the medium is cooled to 20 to 25 DEG C to prepare a sawdust medium.

The method of growing the mushroom using the sterilized sawdust medium as described above is as follows. The sawdust medium according to the present invention can be cultivated on various mushrooms, but preferably includes mushroom.

The thus prepared sawdust medium is inoculated with a mildew of the shiitake in a clean room and then the inoculated medium is cultured at 20 to 25 ° C, preferably at 21 to 22 ° C, at a humidity of 50% or more, preferably 60 to 70% 30 to 60 days, preferably 30 days.

After termination of the dark culture, light cultivation is carried out by providing sunshine for 30 to 60 days, preferably 30 days, in order to cause browning and extend to the entire medium. As described above, browning proceeds on the whole of the culture medium, and then the culture is started.

According to the present invention, the mushroom can be harvested after culturing for about 5 to 8 days, preferably one week, after incidence.

Hereinafter, the present invention will be described in more detail with reference to Examples. It is to be understood, however, that these examples are provided so that the scope of the present invention is not limited thereto.

[Example 1]

1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 28 kg of calcium carbonate, 400 kg of wheat bran, 100 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 1.7 L of nadoxid sulfur and 1.7 L of anthocyanin.

[Example 2]

1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 100 kg of peat moss, 28 kg of calcium carbonate, 400 kg of wheat bran, 100 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 1.7 L of nano-demineralized sulfur and 1.7 L of anthocyanin.

[Example 3]

1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 28 kg of calcium carbonate, 400 kg of wheat bran, 400 kg of cottonseed flour, 24 kg of brown sugar, 1700 L of water, 25 kg of wood vinegar, 1.7 L of sulfurized nano and 1.7 L of anthocyanin.

[Example 4]

1400 pieces of sawdust medium for mushroom cultivation were prepared by blending 2280 kg of oak sawdust, 28 kg of calcium carbonate, 400 kg of cottonseed, 400 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 25 kg of silk peptide, 1.7 L of nano-demolished sulfur and 1.7 L of anthocyanin.

[Example 5]

1400 pieces of sawdust for cultivation of mushrooms were prepared by blending 2280 kg of oak sawdust, 100 kg of peat moss, 28 kg of calcium carbonate, 400 kg of wheat bran, 100 kg of cottonseed, 24 kg of brown sugar, 1700 L of water, 25 kg of wood vinegar, 25 kg of silk peptide, Respectively.

[Experimental Example 1] Cultivation of shiitake mushroom using sawdust medium

The sawdust media prepared in Examples 1 to 5 were placed in a cylindrical plastic bag having a diameter of 14 cm and a height of 12 cm and bundled. The medium container was placed in a sterilizer and sterilized at 100 ° C for 60 minutes at 121 ° C for 90 minutes, and then the medium was cooled to 20 to 25 ° C.

After the inoculated medium was inoculated to the sterilized medium as described above, the inoculated medium was cultured for 30 days at 22 ° C and 60% humidity for 30 days. After the completion of the incubation period, the browning was progressed through the medium for 30 days, followed by incubation for 1 week in a cultivar, and shiitake was harvested.

As a comparative example, when mushroom was cultivated under the same conditions using oak sawdust and commercial sawdust medium containing rice bran as the main raw material, the yield was 10% on average in Example 1, 12% in Example 2, An increase of 14% for Example 4 and 17% for Example 5 was observed.

[Experimental Example 2] Sulfur and anthocyanin content

Compared with the shiitake mushroom according to the comparative example, the mushroom harvested in Examples 1 to 5 was measured for the composition of the sulfamic amino acid and anthocyanin in the mushroom according to the published method, and the results are shown in Tables 1 and 2 same.

(Unit: wet basis, mg / g) Sample Cysteine Methionine Example 1 0.134 0.011 Example 2 0.135 0.010 Example 3 0.135 0.010 Example 4 0.137 0.011 Example 5 0.143 0.020 Example 6 0.058 0.067

(Unit: wet basis, mg / g) Sample Anthosian Example 1 0.023 Example 2 0.025 Example 3 0.024 Example 4 0.033 Example 5 0.051 Example 6 0.003

As described above, cultivation of shiitake mushroom using the sawdust medium according to the present invention resulted in shortening of cultivation period and increase in yield, as well as increased sulfur amino acid and anthocyanin content, so that the sulfur component and anthocyanin were absorbed into the mushroom well .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims. It can be understood that

Claims (7)

Preparing a sawdust medium for mushroom cultivation comprising oak sawdust, calcium carbonate, wheat bran, cotton bark, brown sugar, water, nano-decontamination sulfur and anthocyanin; The method comprising: inoculating the sawdust medium into a culture container, sealing the mixture, heating and cooling the paste medium for sterilization, inoculating the cooled sawdust medium with mushroom seeds, Culturing the mycelium by culturing the mushroom strain in an arm culture; Culturing the mushroom mycelium which has undergone the arm culturing and culturing the mushroom mycelium; And the mushroom mycelium which has undergone the change of browning is introduced into a grower and cultured in a mushroom. The method according to claim 1,
The present invention relates to a method for producing a fermented soybean oil, comprising the steps of: preparing a soybean oil containing 40 to 50% by weight of oak sawdust, 0.1 to 1.0% by weight of calcium carbonate, 5 to 10% by weight of wheat bran, 1 to 5% To 0.1% by weight of anthocyanins and 0.01 to 0.1% by weight of anthocyanins. The method according to claim 1,
And 10 to 20% by weight of peat moss. The method according to claim 1,
And 1 to 5% by weight of a woodpecker. The method of cultivating mushroom using the sawdust medium. The method according to claim 1,
And 1 to 5% by weight of a silk peptide. The method according to claim 1,
A peach moss 10 to 20% by weight, a vinegar solution 1 to 5% by weight, and a silk peptide 1 to 5% by weight. A mushroom cultivated by the method according to any one of claims 1 to 6.

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