KR20160024128A - Steam mushroom ingredients to the Verve conduction method - Google Patents

Abstract

The present invention relates to a method of inducing primordium of Grifola frondosa, and more specifically, to a method of inducing primordium of Grifola frondosa which induces generation of Grifola frondosa under the environment that the inside of a container is not contaminated by bacteria by using a container which includes an inner container containing a mix culture for inoculating seeds of Grifola frondosa and an outer container having a predetermined space and to which a filter is attached, provided in the outside of the inner container. Grifola frondosa is generated in the inner container containing the mix culture for inoculating seeds of Grifola frondosa and polluted external environments are protected by the outer container so that Grifola frondosa is not contaminated by bacteria of external environments and thus high-quality Grifola frondosa can be obtained. In addition, the method induces generation of Grifola frondosa using a mix culture containing one or more sawdusts of oak sawdust, shiitake waste wood sawdust, brewers grain, corncob and poplar sawdust and one or more yield-increasing ingredients selected from wheat bran, dried bean, cashew nut meal, corn gluten feed, red bean peel, potato peel, soybean meal or wheat bran meal, thereby advantageously reducing an induction time of primordium of Grifola frondosa and thus facility costs and management/maintenance costs burdened by producers.

Description

{Steam mushroom ingredients to the Verve conduction method}

The present invention relates to a method for inducing a fresh mushroom using a water-purifying material, and more particularly, to a method for inducing a mushroom of a mushroom, This method induces the generation of leaf mushroom in an environment where the inside of the container is not contaminated with bacteria.

Leaf mushrooms grow in the summer and autumn in the base of hardwoods. The fruiting body of the leaf mushroom is a complex mushroom cluster which is formed by a large number of small mushroom gardens gathered at the end of the branch of the mushroom stand which is divided into several branches. Mushroom shrimps are 2 ~ 5 ㎝ in width and 2 ~ 4 ㎜ in thickness and are semicircular or fan shaped. The leaves are like a leaf, and they are layered together to form a bundle of fruit bodies. The colors are black, dark brown, grayish brown, and white. There is radial fiber pattern and faint ring pattern on the surface of the fresh face, and the bottom hole is white, length is 1 ~ 3㎜, it is on the bag, and the hole is circular. In fall, hardwood species such as oak trees, stems, and dead wood occur at the base of dead wood. In addition, Leaf mushroom has excellent efficacy for lowering blood sugar, is effective for obese people who are rich in insoluble dietary fiber, and has artificial cultivation with edible mushroom with good taste and fragrance.

The cultivation process of leaf mushroom is proceeded with the process of raw material - mixer - mixing - ingestion (bottle cultivation), filling (encapsulation cultivation) - sterilization - cooling - inoculation - culture - culture induction - development - growth - delivery. During the cultivation process, there are many difficulties in the process of induction and development.

At present, it is produced through the cultivation of bottles and bagwood in Kangwon Hwacheon, Gyeongnam Changwon, Yangsan, etc. However, when it is used in large quantities, the mushroom is easily broken and its size is not constant.

In order to solve this problem, the Japanese snow lady company cultivates the microbial cells for 60-70 days after inoculation, and when the microbial cells are formed, the top is cut with a knife, It is cultivated for 40 ~ 50 days by folding the bag at the upper part. When the mushroom is developed, it is cut by cutting with a knife to induce it. However, the formation of the mushroom is not constant and the germination rate is decreased and the growth is not continued. .

Leaf mushroom seeds are weaker than other edible mushroom species and are difficult to cultivate. Especially, hyphae that are weak in early stage are contaminated by external environment, and contaminated hyphae with superficial growth are difficult to grow, so that profitable leaf mushrooms are grown There is a difficult problem.

Japanese Patent Application Laid-Open No. 2003-00066 (2003.07.01) Japanese Patent Laid-Open No. 2003-169540 (Jun. 2003) Japanese Patent Laid-Open No. 2001-120056 (Aug. 5, 2001) Japanese Unexamined Patent Application Publication No. 2000-270675 (Mar. 3, 2000)

It is an object of the present invention to shorten the period of induction without causing fungi to be contaminated during the induction period in a container composed of an inner container and an outer container with a filter attached thereto to solve the above problems.

It is also possible to use one of sawdust and wheat bran, dried bean paste, cashew nutskin, protein bark, red bean husk, potato bark, soybean meal or wheat bran consisting of one or more of oak sawdust, It is an object of the present invention to reduce the period of induction of the mushroom by using a mixed medium in which two or more water-repellent materials are mixed.

In order to accomplish the above object, the present invention provides a method for inducing a fresh mushroom by using a water-purifying material according to the present invention, comprising the steps of: To induce the generation of leaf mushroom in an environment where the inside of the container is not contaminated with bacteria.

The inner container may be made of a plastic, a bottle, or a plastic material. The inner container may have a 3 to 4 cm communication hole communicating with the inner space of the outer container.

The outer container may be made of a plastic or plastic material, and a filter may be attached to the upper surface of the outer container. The filter may be one of a heparin filter, a cotton or a sponge.

In addition, the occurrence of the mushroom of the present invention is caused by sawdust consisting of one or more of oak wood sawdust, oblong wood sawdust, beer mug, cone cob, and poplar sawdust, wheat bran, dried bean curd, cashew nuts foil, protein bodied bean bark, potato bark, Or wheat bran at a dry weight ratio of 7 to 10: 0.9 to 1.2, and the inner container in which the mushroom seedlings have been inoculated is placed in the outer container and sealed to obtain a mushroom Thereby inducing the occurrence of the disease.

Also, the external and internal humidity of the inner container and the outer container is 90-100%, and the temperature is 17-22 ° C, thereby inducing the generation of the mushroom.

The present invention relates to a method for inducing a fresh mushroom of the present invention, in which a mushroom is formed in an inner container containing a mixed medium inoculated with a mushroom of a mushroom, and the outer environment is protected by an outer container, It has the effect of harvesting leaf mushrooms.

It is also possible to use one of sawdust and wheat bran, dried bean paste, cashew nutskin, protein bark, red bean husk, potato bark, soybean meal or wheat bran consisting of one or more of oak sawdust, The use of a mixed medium containing two or more water-repellent materials induces the occurrence of leaf mushroom, thereby shortening the period of induction of the leaf mushroom, thereby reducing the cost of facilities and maintenance expenses incurred by the producer.

1 is a front view showing an inner container, an outer container, a filter, a communication hole, a mixed medium, and a mushroom seedling used in the method for inducing a fresh mushroom according to the present invention.
FIG. 2 is a state diagram showing an inner container, an outer container, a communication hole, and a mixed medium used in the method for inducing the fresh mushroom according to the present invention.
FIG. 3 is a state diagram showing an outer container and a filter in the container used in the method for inducing the fresh mushroom according to the present invention.
FIG. 4 is a state view showing a state where a foot is housed in a room for induction of fresh mushrooms according to the present invention. FIG.
FIG. 5 is a state in which the induction is induced for 25 to 30 days after the inoculation of the mushroom seedlings according to the present invention.
FIG. 6 is a state in which fresh-air induction is performed for 30 to 35 days after the inoculation of the mushroom seedlings according to the present invention.
7 is a state diagram of a mushroom grown as a fruiting body after the induction of the mushroom according to the present invention.

The present invention is a method for inducing the fresh mushroom.

The method of the present invention for inducing the mushroom of the present invention comprises an outer container having a space formed outside the inner container containing the mixed medium for inoculating the seeds of the mushroom, and an outer container to which the filter is attached, It is desirable to induce the occurrence of the mushroom.

Preferably, the inner container is made of a plastic, a bottle, or a plastic material. The outer container may be formed of vinyl or plastic material. The outer container may be formed of a plastic or plastic material. And a filter composed of one of a hepar filter, cotton or a sponge is attached to the upper part of the outer container.

In addition, the occurrence of the mushroom of the present invention is caused by sawdust consisting of one or more of oak wood sawdust, oblong wood sawdust, beer mug, cone cob, and poplar sawdust, wheat bran, dried bean curd, cashew nuts foil, protein bodied bean bark, potato bark, Or wheat bran at a mass ratio of 7 to 10: 0.9 to 1.2 in a ratio of mass of 7 to 10: 0.9 to 1.2, and then the inner container is inoculated with the outer container and sealed to produce the mushroom Lt; / RTI >

In addition, it is preferable that the external and internal humidity of the inner container and the outer container is 90-100% and the temperature is 17-22 ° C to induce the generation of the mushroom.

Hereinafter, preferred embodiments and experimental examples of the present invention will be described in detail with reference to the accompanying drawings. However, it should be understood that the following Examples and Experiments are intended only to illustrate the present invention, but do not limit the scope of the present invention.

FIG. 1 is a front view showing an inner container, an outer container, a filter, a communication hole, a mixed medium, and a mushroom seedling used in the method for inducing the mushroom according to the present invention. FIG. 3 is a state diagram showing an outer container and a filter in a container used in the method for inducing the fresh mushroom according to the present invention. FIG. 3 is a state view showing an inner container, an outer container, a communication hole and a mixed medium used in the method of the present invention.

FIG. 4 is a state view showing a state where the feet are housed in the room for induction of the fresh mushroom according to the present invention, FIG. 5 is a state in which the fresh mushroom of the present invention has undergone a fresh induction for 25 to 30 days after inoculation, FIG. 7 is a state diagram of a mushroom grown as a fruiting body after the induction of the mushroom according to the present invention. FIG.

The method for inducing the fresh mushroom according to the present invention comprises an inner vessel 10 in which a mixed medium 30 for inoculating a seed of a mushroom is inoculated and an outer vessel 20 in which a filter 21 is attached, In the environment that is not contaminated by bacteria, it induces the regeneration through the generation of leaf mushroom.

The size of the inner container 10 is not limited. However, the size of the inner container 10 is preferably 10 to 12 cm , A length of 20 to 22 cm and a height of 15 to 20 cm is preferably used. The size of the bottle is preferably 900 to 1200 ml, and the size of the plastic is 10 to 12 cm and 20 to 22 cm Cm and a height of 15 to 20 cm are preferably used.

The mixed medium 30 contained in the inner container 10 is not limited in its kind, but it is preferable to use sawdust composed of one or more of oak wood sawdust, oblong wood sawdust, beer mug or koncove as the raw material It is preferable to use one or more of wheat bran, dried bean paste, cashew nuts, protein bark, red bean husks, potato bark, soybean meal or wheat bran, and more preferably cashew nuts, wheat bran, Is preferably used. At this time, the mixing medium 30 preferably has a water content of 60 to 70%.

In addition, since the cashew nuts are supplied from Vietnam and India as fruit foil, they serve to enhance the mycelial elongation rate significantly as compared with soybean meal among the above-mentioned water-repellent materials, so it is most preferable to use cashew nuts as the water-purifying material.

The mixed medium 30 may be a sawdust composed of one or more of oak sawdust, oak wood sawdust, beer brew, cone cob, and poplar sawdust, bran, dried bean paste, cashew nut, protein brew, red bean husk, It is preferable to mix the water-purifying material composed of one or two or more of soybean meal or wheat bran at a dry weight ratio of 7 to 10: 0.9 to 1.2. At this time, the mixed medium 30 preferably has a water content of 60 to 70%.

In one embodiment, wheat bran, protein bran, and cashew nuts, which are water-wasting materials, are mixed in a mass ratio of 1: 1: 1 to sawdust mixed with oak wood sawdust and poplar sawdue at a ratio of 5: (30).

Inoculating the mixed mushroom seeds 31 into the mixed medium 30 may be carried out by adding the mushroom seeds of the mushroom to the mixed medium 30 at a mass ratio of 0.9 to 1.5: It is preferable to inoculate.

In one embodiment, it is preferable to inoculate 2.6 to 3.1 kg of the mixed medium (30) mixed with 26 to 31 g of the mushroom seedlings (31).

The inner container 10 containing the mixed medium 30 is sterilized at 114 ° C. for 3 hours or at 121 ° C. for 60 minutes to kill the bacteria contained in the mixed medium 30, (31) is preferably inoculated.

1 and 2, the upper part of the inner container 10 containing the mixed medium 30 in which the mushroom seedlings 31 are inoculated is placed in a communication hole 11 ), So that the mushroom seedlings (31) can breathe and induce the root induction.

The communication hole 11 is preferably formed by a scratch or a hole and the communication hole 11 is formed with a sharp tool such as a knife or the like in the upper portion of the inner container 10.

When the inner container 10 is made of vinyl, the upper portion of the vinyl may be punched to form a hole or the upper portion of the vinyl may be sealed. Then, the communication hole 11 may be formed with a sharp tool such as a knife, When the inner container 10 is bottled, the top of the bottle is covered with plastic or vinyl, and the upper portion of the inner container 10 is covered with a sharp tool such as a knife The upper portion of the plastic is covered with plastic or vinyl and the upper portion of the inner container 10 is covered with a sharp tool such as a knife in a thickness of 3 to 4 cm Is formed in the communication hole (11).

As shown in FIGS. 1 and 3, it is preferable that the outer container 20 is made of vinyl or plastic. The size of the plastic or plastic of the outer container 20 is 10 to 13 cm, 23 cm and a height of 15 to 21 cm is preferably used. At this time, it is preferable that a filter 21 is attached to the upper part of the outer container 20, and it may be attached to the upper side depending on the material of the inner container 10.

Also, as shown in FIGS. 1 and 3, the filter 21 is not limited in its kind, but it is preferable to use one of a HEPA filter, a cotton or a sponge.

The use of the filter 21 prevents the entry and exit of bacteria that can be naturally or inhaled in the container 1, so that the filter 21 can be guided in an environment free from contamination of the mushroom seedlings.

As shown in FIG. 1, it is preferable that the outer container 20 forms a certain space with the inner container 10, and the upper portion of the outer container 20 is completely sealed It is desirable to induce the root mushroom from the foot to the root. At this time, it is preferable that the incubation is carried out for 1 to 20 days at a room temperature of 22 to 27 ° C. and a 70 to 80% carbon dioxide concentration of 2000 ppm or less after inoculation of the mushroom seedlings (31) ) It is preferable to cultivate medium temperature culture room for 25 to 27 days at 20 to 25 days under the condition of 80 to 90% carbon dioxide gas concentration of 2000 ppm or less for the humidity, and the temperature of the yarn in the late phase of the leaf mushroom (31) It is preferable that the culture is carried out for 10 to 15 days at a temperature of ~ 22 ° C under a condition of 90 to 100% carbon dioxide gas concentration of 700 ppm or less.

When the culture medium is subjected to the induction from the culture room, the culture of the mushroom seedlings (31) inoculated into the mixed medium (30) is induced after 15 days, and part of the mycelium inoculated into the mixed medium (30) And a part of the air is discharged to the place where the communication hole 11 of the inner container 10 is formed and the mushroom is generated by the growth point of the air hyphae. As shown in Figs. 5 and 6, It is desirable to induce the mushroom to induce fresh mushrooms.

In addition, after 30 days after inoculating the mixed mushroom seedlings (31) into the mixed medium (30), the mushrooms are grown as a fruiting body as shown in FIG.

In addition, it is preferable that the external and internal humidity of the inner container 10 and the outer container 20 is 90 to 100% and the temperature is 17 to 22 ° C so that the fresh mushroom is induced in the fresh mushroom, It is preferable that the outer container 20 is opened, the inner container 10 is opened, and the inner container 10 is cultured to grow into a fruiting body as shown in FIG.

Example 1: A method of inducing fresh mushroom of the present invention according to the present invention.

Comparative Example 1: Induction method of Japanese mushroom of Japanese snow lady company.

Comparative Example 2: Induction method of mushroom of Japanese mori industry.

Experimental Example 1: Production of leaf mushroom

The amount of mushroom production by the induction method in Example 1, Comparative Example 1, and Comparative Example 2 was examined during the induction period, one year rotation period, one bag production, and one year production after inoculation of the seedling during the same growing harvest period .

division After inoculation
Period of induction Harvest period 1 year rotation cycle Production per bag 1 year production Example 1 25-35 days 30 days 5.6 to 6.0 400 ~ 500g 2240 ~ 3000g Comparative Example 1 60 to 70 days 30 days 3.6 ~ 4.0 650g 2340 ~ 2600g Comparative Example 2 45-50 days 30 days 4.0 to 4.8 400 ~ 500g 1600 ~ 2400g

As shown in Table 1, it can be seen that Example 1 has a shorter induction period than Comparative Examples 1 and 2. As a result, the rotation period for cultivating the mushroom for 1 year was improved, and the yield of the medium containing the mushroom seedlings was similar to that of Comparative Example 3, but the yield of 1 year was higher.

Experimental Example 2: Comparison of the presence or absence of the waxy mushroom mixed medium

In order to test the efficiency of the cashew nut foil as a thickening material of the mushroom mixture of Leaf mushroom, Example 2 in which the mushroom seedlings were inoculated in a mixed medium containing 70 parts by weight of oak sawdust and 30 parts by weight of wheat bran, 70 parts by weight of oak sawdust, 10 parts by weight of wheat bran And 20 parts by weight of protein were inoculated in a mixed medium containing 70 parts by weight of oak sawdust, 10 parts by weight of wheat bran, 10 parts by weight of protein, and 10 parts by weight of cashew nuts, The number of days, average yield and rate of growth of the foot of Example 4 inoculated with the mushroom seedlings were compared.

division Raw materials Increase material Moisture content Sterilization Day of cultivation
(± 2 days 40ea
Average second
Foot
(± 3 days 40 ea
Average) Collection days (± 4 days 40ea average) 40ea
Average quantity Rate of increase
compare kind of oak
sawdust bran Protein blood Cashew nuts Example 2 70% 30% 65% 114 ° C
3 hours 25.2 27.8 56 256g 100% Example 3 70% 10% 20% 65% 114 ° C
3 hours 23.5 26.1 53 298g 116% Example 4 70% 10% 10% 10% 65% 114 ° C
3 hours 22.1 23.4 52 453 g 176%

As shown in Table 2, Example 4, which is a mixed medium containing a thickener such as cashew nut, exhibited a higher growth rate than that of Examples 2 and 3, and the days of culture, days of picking and days of picking were shorter , The average yield of 40ea was about twice as high.

It can be confirmed that by using the above-mentioned water-proof material, the shortening of the period of induction of the mushroom and the rate of increase of the yield are improved.

1: container 10: inner container
11: communication hole 20: outer container
21: Filter 30: Mixed medium
31: Leaf mushroom

Claims (5)

In a method for inducing a fresh mushroom using a water-extracting material,
It is composed of an outer container in which a certain space is formed outside the inner container containing the mixed medium for inoculating the seed of the mushroom, and an outer container to which the filter is attached, thereby inducing the occurrence of the mushroom in an environment in which the bacteria are not contaminated in the container A Method for Inducing Leaf Mushroom Using Wastewater.
The method according to claim 1,
Wherein the inner container is made of a plastic, a bottle, or a plastic material, and the inner container has a communication hole of 3 to 4 cm communicating with the inside of the outer container.
The method according to claim 1,
Wherein the outer container is made of vinyl or plastic material, and a filter composed of one of a hepar filter, cotton or a sponge is attached to an upper part of the outer container.
The method according to claim 1,
The occurrence of the leaf mushroom is caused by sawdust composed of one or more of oak wood sawdust, oblong wood sawdust, beer mug, cone cob, and poplar sawdust, wheat bran, dried bean curd, cashew nuts foil, protein brew, red bean husk, potato bark, The inner container was inoculated with the outer container in which the inner mushroom seedlings were inoculated in a mixed medium prepared by mixing one or two or more of the waxy materials at a dry weight ratio of 7 to 10: 0.9 to 1.2, Wherein said method comprises the steps of:
The method according to claim 1,
Wherein the external and internal humidity of the inner container and the outer container is 90 ~ 100% and the temperature is 17 ~ 22 < 0 > C to induce the generation of the mushroom.

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