KR20110023261A - A discoloration method of fucoidan containing solution, discolorized fucoidan containing solution and cosmetic compositions for sensitive skins containing the same - Google Patents

Abstract

PURPOSE: A method for changing color of fucoidan-containing solution is provided to apply to sensitive skin and to ensure antioxidation without cytotoxicity. CONSTITUTION: A method for changing color of fucoidan-containing solution is performed by irradiating light to the fucoidan-containing solution at 25-60°C for 1-4 hours. The fucoidan is isolated from brown algae, such as Undaria pinnatifida and Laminaria japonica. A method for extracting/purifying brown algae comprises: a step of extracting polysaccharide from brown algae by hot water extraction; a step of removing brown algae solid material from the resultant; a step of selectively removing alginic acid; and a step of desalting, decolorizing, and deodorizing. A cosmetic composition for sensitive skin contains 2-5 weight% of modified fucoidan-containing solution as an active ingredient.

Description

Discoloration method of fucoidan-containing solution, discolored fucoidan-containing solution and cosmetic composition for sensitive skin comprising same {A DISCOLORATION METHOD OF FUCOIDAN CONTAINING SOLUTION, DISCOLORIZED FUCOIDAN CONTAINING SOLUTION AND COSMETIC COMPOSITIONS FOR SENSITIVE SKINS CONTAINING THE SAME}

The present invention relates to a method of discoloring the fucoidan-containing liquid separated from brown algae to be suitable for use as an active ingredient of the cosmetic composition, a fucoidan-containing liquid discolored by the method and a cosmetic composition for sensitive skin comprising the same as an active ingredient.

Rapid environmental changes in modern societies have a significant impact on the lives and diseases of individuals. The concept of sensitive skin is not only increasing the internal and external stimulating factors of the human body but also increasing the standard of living according to the social development of the general public and the recognition of the general public by the development of the beauty industry. The degree to which the skin responds to external irritants, allergens or other environmental factors varies greatly from person to person. Fischer responded to cosmetics by making it more difficult to use any cosmetics, which is called status cosmetics, and Amin and Maybach feel uncomfortable when using cosmetics. In all cases, it was called cosmetic intolerance syndrome, including subjective stimuli, objective stimuli, contact dermatitis, contact thinning, status cosmeticus and dysmorphophobia. According to the survey, about 40% of women in their 20s and 40s in Korea say their skin is sensitive, causing itching, stinging, rashes and redness.

Fucoidan is an effective material for itching, stinging and rashes because it has anti-allergic and anti-inflammatory effects. Fucoidan extracted from brown algae is a sulfated heteropolysaccharide, which is mainly composed of L-fucose with α-1,2 or α-1,3 bonds, and is composed of galactose, mannose, xylose, and gluquus. Carboxylic acid and the like. Fucoidan has been reported to have several pharmacological effects known to be related to the properties of polyanionic charge. For example, anticoagulant, anticancer effect by apoptosis, antiviral (including anti-HIV), anti-inflammatory, anti-allergic effect, and the like. Itching and rash are caused by allergens that stimulate allergen B cells, activating IgE (Immunoglobulin E) antibodies, and IgE stimulating mast cells, which is responsible for the antiallergic effects of fucoidan. Reported results indicate that fucoidan blocks the production of IgE in B cells by blocking signals mediated by NFκB-p52 (Souichi Oomizu, Yuhki Yanase et al, Biochem. And Biophy. Res. Comm., Vol. 350 (3), 24 November 2006, pp. 501-507). Fucoidan also has the ability to remove free radicals (radical scavenger). Free radicals are oxygen radicals such as OH and HO ₂ that have unpaired hole electrons, which are highly reactive and can cause cells to be sensitive, destroying cells, causing aging and irritating the skin. Diseases directly or indirectly associated with free radicals in the body include cancer, leukemia, heart disease, arteriosclerosis, stroke, angina pectoris, hypertension, rheumatism, atopic dermatitis, Parkinson's disease, Alzheimer's, cataracts, dandruff, aging, wrinkles, and blemishes. have.

Therefore, fucoidan has anti-allergic, anti-inflammatory and anti-oxidant effects without side effects, and it is expected to be suitable as a cosmetic material for patients with sensitive skin due to its excellent moisturizing power. However, various functional cosmetics containing fucoidan have been developed, but are separated from brown algae. Since fucoidan has a dark green color, there is a problem in that it is difficult to manufacture a pure white cosmetic.

The present invention has been made to solve the above problems, the present invention is a method for discoloring the dark green fucoidan-containing liquid separated from brown algae suitable for the production of pure white cosmetics and the fucoidan discolored by the method The purpose is to provide a liquid.

It is another object of the present invention to provide a cosmetic composition for sensitive skin comprising the fucoidan-containing solution discolored by the discoloration method as an active ingredient.

Discoloration method of the fucoidan-containing liquid of the present invention for achieving the above object is characterized in that the fucoidan-containing liquid extracted from brown algae irradiated for 1 to 4 hours at a temperature of 25 to 60 ℃.

In one preferred embodiment of the present invention, the brown algae is selected from the group consisting of seaweed spores, kelp, 톳 and Okinawa imitation.

In one preferred embodiment of the invention, the extractive tablet comprises the following steps:

(1) hydrothermal extraction of polysaccharides from brown algae;

(2) a filtration step of removing brown algae solids from the result of step (1);

(3) selectively removing alginic acid from the result of step (2);

(4) desalting, decolorizing and deodorizing the result of step (3).

In a preferred embodiment of the present invention, the step (1) is carried out by immersing and stirring brown algae in water of 80 to 100 ℃ for 1 to 3 hours.

In one preferred embodiment of the present invention, step (3) is carried out by adding CaCl ₂ solution to the product of step (2).

In one preferred embodiment of the invention, step (4) is carried out via ultrafiltration.

In one preferred embodiment of the invention, the light is sunlight or electric lighting.

In one preferred embodiment of the present invention, the discolored fucoidan-containing liquid is yellow.

In one preferred embodiment of the present invention, the discolored fucoidan-containing liquid is orange.

In addition, the fucoidan-containing liquid of the present invention for solving the above problems is characterized in that it is produced by the discoloration method.

In addition, the cosmetic composition for sensitive skin of the present invention for solving the above problems is characterized in that it comprises a discolored fucoidan-containing liquid prepared by the discoloration method of the fucoidan-containing liquid of the present invention as an active ingredient.

In one preferred embodiment of the present invention, the content of the discolored fucoidan-containing liquid of the present invention in the cosmetic composition for sensitive skin is 2 to 5% by weight.

In a preferred embodiment of the present invention, the cosmetic composition for sensitive skin includes bees wax, xanthan gum, citric acid, aloe extract, beta-glucan, broccoli extract, squalene, apricot kernel oil, jojoba seed oil, aloe extract, pasqueflower root It further comprises one or more additives selected from the group consisting of extracts, algins, rosemary extracts, rice extracts, cabbage leaf extracts, esnean extracts, tomato extracts, tomato leaf extracts, tomato stem extracts and turnip leaf extracts.

The cosmetic composition for sensitive skin comprising the fucoidan-containing liquid prepared according to the present invention as an active ingredient can be produced in a pure white color, so that it is highly commercially available as cosmetics, has no cytotoxicity, exhibits antioxidant activity, and has excellent effects on sensitive skin. Indicates.

Hereinafter, the present invention will be described in detail.

The discoloration method of the fucoidan-containing liquid according to an embodiment of the present invention is characterized in that the fucoidan-containing liquid extracted from brown algae is irradiated for 1 to 4 hours at a temperature of 25 to 60 ℃.

The brown alga is preferably selected from the group consisting of seaweed spore leaf, seaweed, seaweed and Okinawa imitation, more preferably seaweed spore leaf or kelp. However, brown algae that can be used is not limited to those listed above, any brown algae containing fucoidan can be used.

The extraction tablet preferably comprises the following steps:

(1) hydrothermal extraction of polysaccharides from brown algae;

(2) a filtration step of removing brown algae solids from the result of step (1);

(3) selectively removing alginic acid from the result of step (2);

(4) desalting, decolorizing and deodorizing the result of step (3).

The step (1) is immersed and stirred in brown algae, preferably seaweed spores or kelp in water at 80 to 100 ℃ for 1 to 3 hours, preferably 100 ℃ for 2 hours to polysaccharides such as alginic acid, fucoidan Extract.

When the extraction is performed at a condition lower than the temperature and time, a sufficient extract cannot be obtained. When the extraction is performed at a condition higher than the temperature and the time, the amount of the extract is too small due to evaporation of water, thereby performing a purification process. Difficulties arise.

There is no particular limitation on the type of filtration or the filtration method used in the filtration process of step (2), and it is sufficient to be able to cleanly remove remaining solids after hydrothermal extraction of brown algae.

In step (3), CaCl ₂ solution is added to a polysaccharide mixture such as alginic acid and fucoidan to gel the alginic acid, which is then removed by centrifugation or filter press.

The step (4) is carried out through ultrafiltration, and the alginate is removed and the resultant is desalted, deodorized and decolorized in an ultrafilter to remove strong aromas and colors of brown algae to improve palatability.

NaCl, CaCl through ultrafilter₂ In the desalting process for removing the back, it is preferable to use a Pall UF membrane having a cutoff of 5,000 to 10,000 during ultrafiltration. In addition, the deodorization and decolorization process of the fucoidan palatability improvement process using an ultrafilter is preferably a Pall company UF membrane having a cutoff of 100,000 to 500,000, which is different from the cutoff of the membrane used in the desalting process.

The desalting process and the deodorizing and decolorizing process, which improves palatability, are separated into the fucoidan-containing solution of the present invention and the residual concentrate having a high molecular weight, and the residual concentrate is darker in flavor and color than the ultrafiltration solution. The problem of the desalination and palatability improvement process by ultrafiltration is that the yield of the fucoidan-containing liquid is lowered to 20 to 50%, but the fucoidan-containing liquid which has undergone such desalting and palatability improvement is more suitable for use as a raw material of cosmetics. Done.

Despite the palatability improvement process, the fucoidan-containing liquid still has problems of color and fragrance for use as a cosmetic ingredient. The smell of algae, such as brown algae, can be easily eliminated by adding appropriate fragrances in the manufacture of cosmetics. However, cosmetics, especially cream products should have a white color, the dark green of the fucoidan-containing liquid is not easily removed by other ingredients, making it difficult to produce a pure white cosmetics.

The present invention was to produce a pure white cosmetic by discoloring the dark green color of the fucoidan solution by a light irradiation method to solve this problem.

Preferably, the light is sunlight or electric lighting. The discoloration process is carried out for 1 to 4 hours at 25 to 60 ℃, if the temperature is less than 25 ℃ has a problem of insufficient discoloring effect, if the temperature exceeds 60 ℃ fucoidan is likely to deteriorate Not desirable In addition, when the discoloration time is less than 1 hour, there is a problem that the discoloring effect is insufficient, and when it exceeds 4 hours, fucoidan may deteriorate, which is not preferable.

The discolored fucoidan-containing liquid is preferably yellow-based, more preferably orange-yellow.

The discolored fucoidan-containing liquid is suitable for producing a pure white cosmetic, and the fucoidan-containing liquid of the present invention is prepared by the discoloration method and then used as an active ingredient of a cosmetic composition for sensitive skin.

It is preferable that the content of the discolored fucoidan-containing liquid of the present invention in the cosmetic composition for sensitive skin is 2 to 5% by weight. If the content is less than 2% by weight, the effect on sensitive skin that is the object of the present invention cannot be sufficiently exhibited. There is a problem, when the content is more than 5% by weight compared with the case of 5% by weight, there is no difference in the sensitive skin soothing effect caused by fucoidan is not preferable.

In addition, the cosmetic composition for sensitive skin of the present invention includes beeswax, xanthan gum, citric acid, aloe extract, beta-glucan, broccoli extract, squalene, apricot kernel oil, jojoba seed oil, aloe extract, pasqueflower root extract, algin, rosemary It is preferable to further include at least one additive selected from the group consisting of extracts, rice extracts, cabbage leaf extracts, Ersony extracts, tomato extracts, tomato leaf extracts, tomato stem extracts and turnip leaf extracts.

Aloe extract has anti-inflammatory and soothing effects, beta-glucan has an immune function, broccoli extract or squalene plays a role in skin softening, and apricot kernel oil extracted from apricot is a skin softening agent and surfactant. Jojoba seed oil has the effect of promoting skin regeneration, aloe extracts have an excellent antioxidant effect, pasqueflower root extract has an excellent anti-inflammatory effect, beeswax and xanthan gum act as a binder and emulsion stabilizer, Algin extracted from seaweed has excellent thickener and binder effect, rosemary extract or citric acid acts as a natural preservative, rice extract nourish the skin, cabbage leaf extract acts as a skin softening agent, Effective against antibacterial and anti-inflammatory drugs, tomato fruit / leaf / stem extract And help increase strength, Turnip leaf extract is effective in preventing aging and to promote collagen formation.

Among the components, the water-soluble component is preferably 10 to 30% by weight, the oil phase component is 20 to 40% by weight, and the fucoidan-containing liquid and the components may be uniformly mixed at a temperature of preferably 70 to 75 ° C. to prepare cosmetics. have.

Hereinafter, the present invention will be described in more detail with reference to Examples.

Example  One. Fucoidan  Stability Test as a Cosmetic Material ( MTT assay )

By examining the cytotoxicity of fucoidan, we examined how suitable fucoidan is as a cosmetic material for sensitive skin. Human fibroblasts were aliquoted into 96 well plates at a concentration of 1.0 × 10 ⁴ clls / well and incubated for 15 hours. The 96 wells were treated with fucoidan solution at concentrations of 6. 25, 12.5, 25, 50 and 100 μl / ml and incubated for 24 hours in a CO ₂ incubator. After 10 μl of MTT (3- (4,5-dimethylthiazol-2yl) -2,5-diphenyl-2H-tetrazolium bromide) solution was added and after 4 hours the supernatant was removed and 150 μl of DMSO (dimethyl sulfoxide) was added. The cell viability was calculated by measuring the absorbance at 550 nm using a microplate reader. Cell viability according to each fucoidan concentration is shown in Table 1 below. As can be seen from the table below, 92.7% was observed even at a concentration of 100 μl / ml, indicating that Fucoidan's toxicity to cells was weak, making it suitable as a cosmetic material for sensitive skin.

   Table 1. Cell survival rate according to fucoidan concentration

Example  2. Antioxidant effect or active oxygen removal effect experiment

1) DPPH experiment

The antioxidant effect of fucoidan was confirmed by DPPH (1.1-diphenyl-2-picrylhydrazyl) experiment. The antioxidant powers of fucoidan and vitamin C were compared. After making fucoidan and vitamin C at concentrations of 6.25, 12.5, 25, 50, and 100 μl / ml, respectively, 100 μl of DPPH solution and 200 μl of fucoidan or vitamin C solution were reacted at room temperature for 30 minutes, and then absorbed at 520 nm with a microplate reader. Measured. The result of measuring the antioxidant degree by the following formula is shown in Table 2 below.

% Antioxidant = [1- (Exp. -Blank) / Control] x 100

At low concentrations of 25 μl / ml or less, fucoidan was found to be an excellent antioxidant with vitamin C.

   Table 2. Antioxidant Degrees of Fucoidan and Vitamin C

2) Comparison of free radical scavenging ability with ceramic antioxidant

Putting Nafion, a polymer membrane, into the Fenton's solution Oxygen radicals (· OH, HO ₂ ·) attack the polymer membrane and the polymer chain is broken, so that F ⁻ ions are present in the solution. By measuring the ion concentration at this time, generation of oxygen radicals and removal of oxygen radicals can be known. In this experiment, Mn, Ce compound and fucoidan used as Nafion membrane and radical scavenger in Fenton solution were put separately to confirm the radical removal effect of fucoidan. Nafion 112 20 × 20mm was added to the Fenton solution (H ₂ O ₂ 10% + Fe ²⁺ 4ppm), 2g of radical scavengers were added thereto, and after 24 hours of mixing, the concentration of fluorine ion was measured using a fluorine ion measuring device (ISE Meter, Ion Selective). Electrode Meter, PH-250L, ISTEK, Inc.) was used for the measurement. The measurement result is shown in FIG. As shown, fucoidan has lower radical scavenging ability than other radical scavengers, but only 0.062 ppm of fluorine ions were measured compared to 0.157 ppm of the Fenton solution itself without the radical scavenging agent. In other words, it is lower than the active oxygen remover of the ceramic type, but showed a very high active oxygen removal effect as a polysaccharide.

Example  3. Cosmetic Ingredients and Content Examples

To the ingredients and contents shown in Table 3 below it was uniformly mixed at a temperature of 70 ℃ to prepare a cosmetic for sensitive skin of the present invention.

Table 3. Components and Compositions of Sensitive Skin Cosmetics

Example  4. Sensitive Skin  Patient Application Test Results

There were 3 men (6%) and 47 women (94%) in sensitive skin patients who visited the dermatology department.The age group was 6 teenagers (12%), 8 teenagers (16%), and 30s. Seventeen (34%), thirteen thirteen (26%) and sixty (over 50s) (12%) were the most common. Three subjects with systemic diseases were hypertension and diabetes mellitus, 25 dry skin (50%), 2 complex skin (4%), and 23 (46%) oily skin (see Table 4 below). . The skin color was 12 light skin (24%), 9 dark skin (18%), and 29 normal skin (58%).

Table 4. Sensitive Skin Distribution

The effects before and after the use of fucoidan-containing cosmetics were improved 24 patients (48%), significantly improved 2 patients (4%), unchanged 21 patients (42%), and worsening 3 patients (6%). Exposure to daylight (see FIG. 2).

The effects of before and after the use of fucoidan-containing cosmetics according to skin dryness and color tone were improved and improved significantly, which was effective in 15 oily skin (65.2%) and 11 dry skin (44%). 17 patients (58.6%) had normal skin and 9 patients (66.7%) had dark skin (Table 5, Table 6).

  Table 5. Effect of before and after use of fucoidan-containing cosmetics according to skin dryness

 Table 6. Effects before and after using fucoidan-containing cosmetics according to skin tone

Fucoidan derived from brown algae has been shown to be effective in various immune disorders, but no studies have been conducted to investigate the effects of allergen-induced sensitive skin patients.

As a result of confirming through the present embodiment, the effect was effective in 52% of the subjects, and this effect is very high for sensitive skin patients. In addition, it improved in both oily and dry skin, and the effect was better in normal skin and dark skin.

Although the invention has been described with reference to the preferred embodiments and the accompanying drawings, different embodiments may be constructed within the spirit and scope of the invention. Therefore, the scope of the present invention is defined by the appended claims, and should be construed as not limited to the specific embodiments described herein.

Figure 1 shows a graph comparing the active oxygen removal power of fucoidan and ceramic antioxidant.

Figure 2 shows the effect of using the fucoidan-containing cosmetics in a graph.

Claims (13)

A method of discoloration of a fucoidan-containing liquid, wherein the fucoidan-containing liquid extracted and purified from brown algae is irradiated with light at a temperature of 25 to 60 ° C. for 1 to 4 hours. The method of claim 1, The brown alga is a method for discoloration of the fucoidan-containing liquid, characterized in that at least one selected from the group consisting of seaweed spores, kelp, 톳 and Okinawa imitation. The method of claim 1, The method of discoloration of the fucoidan-containing liquid, characterized in that the extraction and purification comprises the following steps: (1) hydrothermal extraction of polysaccharides from brown algae; (2) a filtration step of removing brown algae solids from the result of step (1); (3) selectively removing alginic acid from the result of step (2); (4) desalting, decolorizing and deodorizing the result of step (3). The method of claim 3, The step (1) is a method of discoloration of the fucoidan-containing liquid, characterized in that the brown algae are immersed and stirred in water of 80 to 100 ℃ for 1 to 3 hours. The method of claim 3, Step (3) is a color change method of the fucoidan-containing liquid, characterized in that the addition of CaCl ₂ solution to the product of step (2). The method of claim 3, Step (4) is a method of discoloration of the fucoidan-containing liquid, characterized in that carried out through ultrafiltration. The method of claim 1, The light discoloration method of the fucoidan-containing liquid, characterized in that the sunlight or electric lighting. The method of claim 1, The discoloration method of the fucoidan-containing liquid, characterized in that the discolored fucoidan-containing liquid is yellow. The method of claim 8, A discoloration method of a fucoidan-containing liquid, wherein the discolored fucoidan-containing liquid is orange. A fucoidan-containing liquid, characterized by discoloration by the method of any one of claims 1 to 9. A cosmetic composition for sensitive skin comprising the fucoidan-containing liquid according to claim 10 as an active ingredient. The method of claim 11, The content of the fucoidan-containing liquid is a cosmetic composition for sensitive skin, characterized in that 2 to 5% by weight. The method of claim 11, Bead wax, xanthan gum, citric acid, aloe extract, beta-glucan, broccoli extract, squalene, apricot kernel oil, jojoba seed oil, aloenier extract, pasqueflower root extract, algin, rosemary extract, rice extract, cabbage leaf extract, earth A cosmetic composition for sensitive skin further comprising at least one additive selected from the group consisting of near extract, tomato extract, tomato leaf extract, tomato stem extract, and turnip leaf extract.

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