CN106038355A - Application of highly sulfated seaweed polysaccharide to post-basking repair mask - Google Patents
Application of highly sulfated seaweed polysaccharide to post-basking repair mask Download PDFInfo
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- CN106038355A CN106038355A CN201610575483.7A CN201610575483A CN106038355A CN 106038355 A CN106038355 A CN 106038355A CN 201610575483 A CN201610575483 A CN 201610575483A CN 106038355 A CN106038355 A CN 106038355A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/004—Aftersun preparations
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Abstract
The invention provides application of highly sulfated seaweed polysaccharide to a post-basking repair mask. The application means that the highly sulfated seaweed polysaccharide can be applied to preparation of the post-basking repair mask. Raw materials of the post-basking repair mask are all natural materials, non-toxic, and free of stimulation and side effects; the highly sulfated seaweed polysaccharide is relatively high in effects of moisture absorption, moisture preservation, oxidation resistance and inflammatory resistance, can provide sufficient moisture for skin, can form a moisturizing membrane to retain moisture, and can calm exhausted and rough skin, remove superoxide anion free radicals in the skin, and alleviate skin inflammation. The mask can be applied to post-basking facial skin care.
Description
Technical field
The present invention relates to cosmetic technical field, be specifically related to a kind of high Sulfated Sargassum polysaccharides in Post-basking repairing mask
In application.
Background technology
Ultraviolet in sunlight is in all exogenous cause aging factors of skin, effect maximum, cumulative bad the strongest, because of
This, the extrinsic aging of skin is referred to as " photoaging ".The injury that skin is caused by ultraviolet is broadly divided into erythema, melanism, skin
Skin is aging and photosensitivity reaction.We be commonly referred sun-proof be basically uv blocking A and UVB.The penetration power of UVA is extremely strong,
Skin surface can be passed through, be deep into the skin corium of skin, cause the dehydration at the bottom of flesh, stimulate the cell of skin corium to produce melanin
Pile up.UVB wavelength is shorter, and penetration power is relatively strong, epidermis can have been caused for a long time red and swollen more serious there will be excoriative existing
As.
Summer, temperature was high, ultraviolet is strong, sun-proof become the cardinal task of skin protection, some have argued that and wipe sunscreen cream with regard to ten thousand
Thing is very lucky, and after-sun can not be ignored very much in fact.Only carrying out Sun Care, skin does not has the rubescent or tanned of immediacy, but
It is not represent skin just to escape urging of sunlight residual, wipes the sun-proof articles that SPF (sun protection factor) is high simultaneously, easy blocked pore, unfavorable
In perspiring, so that the epidermis of holding moisture of skin is imperfect so that moisture easily runs off, and skin becomes dry.Therefore, by the sun
Skin after solarization all can be in serious exsiccosis, has the calmest reparation only and keeps the skin wet, could remain unchanged after solarization
Have skin quality.
Post-basking repairing mask is the after-sun method of the most quick most convenient, and safely and effectively Post-basking repairing mask should be simultaneously
Meet claimed below: 1. the deep water supplement nursing of skin is provided rapidly, advance the abundant infiltration of activity moisturizing ingredient；2. the most clear
The factor of skin aging is caused except ultraviolet irradiates the oxygen-derived free radicals etc. produced；3. there is certain antiinflammatory action, can be calm
Releive solarization after flesh, alleviate red swelling of the skin pruritis；4. effectively suppression melanin is formed, and lowers mottle；The most natural non-stimulated, protect
Protect the sensitive skin after ultraviolet irradiates.
In the market Post-basking repairing mask be mainly some can the moisture-absorbing moisture-keeping class facial film of quick moisturizing, general absence
Remove the effect such as free radical, antiinflammatory.We have discovered that high Sulfated Sargassum polysaccharides has preferable moisture-absorbing moisture-keeping, antioxidation
And antiinflammatory action, on the one hand can be that skin provides adequate water, and lock moisture film holding moisture can be formed, the most permissible
Calm skin tired, coarse, removes the ultra-oxygen anion free radical in skin, alleviates scytitis.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, it is provided that a kind of high sulphation with moisture-absorbing moisture-keeping antioxidation anti-inflammatory properties
Sargassum polysaccharides application in Post-basking repairing mask.
For achieving the above object, the technical solution used in the present invention is: high Sulfated Sargassum polysaccharides is in after-sun face
Application in film, the Sulfated Sargassum polysaccharides of described height can apply to prepare Post-basking repairing mask.The Sulfated sea of described height
Polysaccharides application in Post-basking repairing mask refers to that this facial film can apply to the sun and is exposed to the sun the nursing of rear face skin.
Wherein, described height Sulfated Sargassum polysaccharides sulfate content is 20-45%, and molecular weight is 12,000-87,
000Da。
Wherein said height Sulfated Sargassum polysaccharides application in Post-basking repairing mask, it is characterised in that described facial film
Liquid includes following parts by weight raw material: high Sulfated Sargassum polysaccharides 1-10 part, glycerol 5-15 part, olive oil 3-10 part, dimension are given birth to
Element B5 1-5 part, deionized water 60-90 part.
Wherein, described facial film preparation method comprises the steps:
(1) the high Sulfated Sargassum polysaccharides solution of preparation 1-8%.
(2) in solution, add the stirring of a certain amount of vitamin B5, after dissolving, add a certain amount of glycerol and olive oil stirring.
(3) paper membrane is put into immersion 1h in facial mask liquid, must have the facial film of after-sun function.
Wherein, the application in Post-basking repairing mask of the described height Sulfated Sargassum polysaccharides derives from Phaeophyta Laminariales
With Fucales Sargassum.
The invention discloses the Sulfated Sargassum polysaccharides of described height the experiment in vivo and vitro of skin repair function after shining is measured
Show that it has significant moisturizing, antioxidation and antiinflammatory action to skin, skin is had no stimulation.
The assay method of the moisturizing test of above-mentioned Post-basking repairing mask is: test environment conditions: test environment temperature is 22
DEG C ± 1 DEG C, humidity is 50% ± 5%.Volunteer requires: effectively volunteer at least 30 people；Age is between 20 years old～65 years old；Before
The basic value of arm test zone capacitance method moisture of skin analyzer is between 15 years old～45；Disease without serious systemic, nothing immunity lack
Fall into or autoimmune disease person；Non-activity anaphylactic disease person；Previously to skin-care cosmetics without severe allergy history person；Closely
Hormone medicine and immunosuppressant person had not been used in 1 month；Do not participate in other clinical trial persons；Use according to regulations tested material
And all information；Before test, all volunteers should fill in Informed Consent Form.
Recipient site can not use any product (cosmetics or topical drug) for first 2 days～3 days.Before test, experimenter needs
Survey in both hands forearm to be cleaned, by dry medicated napkin wiped clean.After cleaning, survey in experimenter's both hands forearm and carry out measurement zone
Field mark.Before official testing, it should sit quietly in standard compliant room at least 30min, it is impossible to drinks water, forearm exposes, in survey
Examination state is placed, and keeps loosening.Testing procedure: in experiment, labelling 3cm × 3cm pilot region, same arm inside left and right arm
Can the simultaneously multiple region of labelling, interregional be all randomly dispersed in left and right arm every 1cm, test product and comparison.Capacitance method skin
Skin analyzer carries out the measurement of tested region and control zone, and each region is according to parallel assay 5 times.First measure each test zone
Blank value, then face mask film 15min, when measuring 1h, 2h, 4h and 8h respectively, tested region and the skin in blank region
Water content.Test result indicate that the facial film that with the addition of high sulphation Sargassum polysaccharides has preferable moisturizing Water-saving effect.
The assay method of the irritation test of above-mentioned Post-basking repairing mask is: take white rabbit 10, respectively at its buttocks
Choosing area at portion is each one piece of 3cm × 3cm size skin, after shears cropping, loses hair or feathers with sodium sulfide, scrapes off gently, with clear
Water rinse wipe dry, random point two groups, often group 5, next day Post-basking repairing mask, normal saline respectively the most once, every time
15min, washes away with warm water after each face mask film, applies altogether 3 times, examines the dermoreaction of 12,24,48,72 hours, as red
Speckle, hyperemia, edema, hemorrhage, rotten to the corn and ulcer etc..Test result indicate that the facial film that with the addition of high sulphation Sargassum polysaccharides is to rabbit
Skin is without any stimulation.
The assay method of above-mentioned antioxidation antiinflammatory experiment is: be 1x10 by density6By HaCaT cell seeding in new containing 10%
In the 100mL culture bottle of the DMEM height sugar culture fluid of raw Ox blood serum and dual anti-(100U/mL penicillin, 100mg/L streptomycin), in
37 DEG C contain 5%CO2Cell culture incubator in cellar culture, when cell growth reach 80-90% merge time, be finished after cell dissociation
Uniformly, cell counting count board counts, and cell is inoculated in 24 orifice plates, inoculum density 5*10 in training piping and druming4, every hole 1mL.37 DEG C, 5%CO2
Incubator is cultivated 24h.The high Sulfated Sargassum polysaccharides (SF) of application variable concentrations protects 24h in advance, then carries out UVA irradiation,
Experiment is divided into Normal group, UVA group, the SF+UVA group of variable concentrations and positive controls.Test after terminating according to test kit
The content of SOD and NO in operating instruction detection cell culture fluid.SF process group can significantly increase the clearance rate of ROS, its
Middle high dose group effect is preferable, and the clearance rate of its ROS, up to 90%, is better than positive control Vc group.Each SF process group cell
With the content of NO in a certain degree of reduction cell culture fluid, its middle and high concentration group effect is best, and the content of its NO is close to normal
Level.Show that sample SF has good antioxidation and antiinflammatory action.
The invention discloses the application in preparation Post-basking repairing mask of a kind of high Sulfated Sargassum polysaccharides.Face of the present invention
The raw material that film uses is natural material, nontoxic, non-stimulated, have no side effect；High Sulfated Sargassum polysaccharides has preferably suction
Wet moisturizing, antioxidation and antiinflammatory action, can be that skin provides adequate water, and can form lock moisture film holding moisture, simultaneously
Can calm skin tired, coarse, remove the ultra-oxygen anion free radical in skin, alleviate scytitis.This facial film can be answered
It is exposed to the sun for the sun nursing of rear face skin.
Accompanying drawing explanation
Fig. 1 is that high sulphation Sargassum polysaccharides Post-basking repairing mask 1-3 and commercially available facial film are to skin moisturizing activity examination knot after shining
Fruit figure；
Fig. 2 is the high sulphation Sargassum polysaccharides clearance test figure to HaCaT cell ROS；
Fig. 3 be high sulphation Sargassum polysaccharides in HaCaT cell culture fluid NO affect Test Drawing.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention
Rather than restriction the scope of the present invention.In addition, it is to be understood that after having read present disclosure, those skilled in the art can
To make various changes or modifications the present invention, these equivalent form of values fall within limited range of the present invention equally.
Embodiment 1: the preparation of high sulphation Sargassum polysaccharides Post-basking repairing mask 1
A kind of high Sulfated Sargassum polysaccharides Post-basking repairing mask, by following parts by weight raw material: high Sulfated Sargassum
Polysaccharide 2 parts, glycerol 5 parts, 5 parts of olive oil, vitamin B5 3 parts, deionized water 85 parts.
Described facial film preparation method comprises the steps:
(1) preparation 2% high Sulfated Sargassum polysaccharides solution 85mL.
(2) in solution, a certain amount of 3g vitamin B5 is added, stirring, add 5mL glycerol after dissolving and 5mL olive oil stirs
Mix.
(3) paper membrane is put into immersion 1h, high sulphation Sargassum polysaccharides Post-basking repairing mask 1 in facial mask liquid.
Embodiment 2: the preparation of high sulphation Sargassum polysaccharides Post-basking repairing mask 2
A kind of high Sulfated Sargassum polysaccharides Post-basking repairing mask, by following parts by weight raw material: high Sulfated Sargassum
Polysaccharide 5 parts, glycerol 7 parts, 8 parts of olive oil, vitamin B5 3 parts, deionized water 77 parts.
Described facial film preparation method comprises the steps:
(1) preparation 5% high Sulfated Sargassum polysaccharides solution 77mL.
(2) in solution, a certain amount of 3g vitamin B5 is added, stirring, add 7mL glycerol after dissolving and 8mL olive oil stirs
Mix.
(3) paper membrane is put into immersion 1h, high sulphation Sargassum polysaccharides Post-basking repairing mask 1 in facial mask liquid.
Embodiment 3: the preparation of high sulphation Sargassum polysaccharides Post-basking repairing mask 3
A kind of high Sulfated Sargassum polysaccharides Post-basking repairing mask, by following parts by weight raw material: high Sulfated Sargassum
Polysaccharide 8 parts, glycerol 6 parts, 6 parts of olive oil, vitamin B5 2 parts, deionized water 78 parts.
Described facial film preparation method comprises the steps:
(1) preparation 8% high Sulfated Sargassum polysaccharides solution 78mL.
(2) in solution, a certain amount of 2g vitamin B5 is added, stirring, add 6mL glycerol after dissolving and 6mL olive oil stirs
Mix.
(3) paper membrane is put into immersion 1h, high sulphation Sargassum polysaccharides Post-basking repairing mask 1 in facial mask liquid.
Embodiment 4: the test of high sulphation Sargassum polysaccharides Post-basking repairing mask moisturizing activity
Test sample: high sulphation Sargassum polysaccharides Post-basking repairing mask and commercially available Post-basking repairing mask in embodiment 1,2 and 3
Test method: test environment conditions: test environment temperature is 22 DEG C ± 1 DEG C, and humidity is 50% ± 5%.Volunteer
Require: effectively volunteer at least 30 people；Age is between 20 years old～25 years old；Disease without serious systemic, without immunodeficiency or self
Immune disease person；Non-activity anaphylactic disease person；Previously to skin-care cosmetics without severe allergy history person；In nearly 1 month
Hormone medicine and immunosuppressant person were not used；Do not participate in other clinical trial persons；Use according to regulations tested material and data
Complete；Before test, all volunteers should fill in Informed Consent Form.
Recipient site can not use any product (cosmetics or topical drug) for first 2 days～3 days.Before test, experimenter exists
Survey in needing to clean both hands forearm, by dry medicated napkin wiped clean.After cleaning, survey in experimenter's both hands forearm and carry out 3cm
× 3cm pilot region measured zone labelling.Exposed arm skin shines 1 hour under the outdoor 25-28 degree fine day sun, and test is produced
Product and comparison are all randomly dispersed in left and right arm.Capacitance method skin analyzer carries out the measurement of tested region and control zone,
Each region is according to parallel assay 5 times.First measure the blank value of each test zone, then face mask film 15min, measure respectively 1h,
When 2h, 4h and 8h, tested region and the moisture content of skin in blank region.
Result: facial film shows (Fig. 1) to skin moisturizing activity experiment result after shining, skin after shining is had by each test facial film
Certain moisturizing effect, wherein, the moisturizing effect of facial film 1-3 prepared by the embodiment of the present invention is better than commercially available facial film, wherein facial film 3
Behave oneself best.Meanwhile, the moisturizing effect of facial film is relevant with the time.After face mask film 1-4h, prolongation in time, facial film moisturizing effect
Being held essentially constant, illustrate that each facial film has certain Water-saving effect, after 8h, each facial film moisturizing effect reduces, the most commercially available
Film fall is maximum, illustrate that the water conservation of facial film 1-3 prepared by embodiment is locked water effect and is better than commercially available facial film.Test result indicate that
The facial film that with the addition of high sulphation Sargassum polysaccharides has preferable moisturizing Water-saving effect.
Embodiment 5: high sulphation Sargassum polysaccharides Post-basking repairing mask irritation test
Test sample: high sulphation Sargassum polysaccharides Post-basking repairing mask and commercially available Post-basking repairing mask in embodiment 1,2 and 3
Test method: the assay method of the irritation test of Post-basking repairing mask is: take white rabbit 10, exist respectively
Choosing area at its buttocks is each one piece of 3cm × 3cm size skin, after shears cropping, loses hair or feathers with sodium sulfide, scrapes off gently,
Rinse with clear water wipe dry, random point two groups, often group 5, next day Post-basking repairing mask respectively the most once, each 15min, often
Wash away with warm water after secondary face mask film, apply altogether 3 times, examine the dermoreaction of 12,24,48,72 hours, such as erythema, hyperemia, water
Swollen, hemorrhage, rotten to the corn and ulcer etc..
Result: high sulphation Sargassum polysaccharides Post-basking repairing mask irritation test the results are shown in Table 1, respectively tests facial film
12h, 24h, 48h, 72h observe all without positive skin reactions such as obvious erythema, hyperemia, edema, hemorrhage, rotten to the corn, ulcer, show this
3 sections of facial films prepared by inventive embodiments to rabbit skin without any stimulation.
Table 1 high sulphation Sargassum polysaccharides Post-basking repairing mask irritation test result
Note: "-" represents Absence of physical signs, " ± " show slight sign, "+" show light sign, " ++ " shows weight sign
Embodiment 6: the test of high sulphation Sargassum polysaccharides antioxidation anti-inflammatory activity
Test sample: high sulphation Sargassum polysaccharides SF
Test method: the assay method of above-mentioned antioxidation antiinflammatory experiment is: be 1x10 by density6By HaCaT cell seeding
In containing 10% new-born calf serum and the 100mL of the DMEM height sugar culture fluid of dual anti-(100U/mL penicillin, 100mg/L streptomycin)
In culture bottle, in 37 DEG C containing 5%CO2Cell culture incubator in cellar culture, when cell growth reach 80-90% merge time, will be thin
Being finished training piping and druming after born of the same parents' digestion uniformly, cell counting count board counts, and cell is inoculated in 24 orifice plates, inoculum density 5*104, every hole
1mL.37 DEG C, 5%CO2Incubator is cultivated 24h.The high Sulfated Sargassum polysaccharides (SF) of application variable concentrations protects 24h, so in advance
After carry out UVA irradiation, experiment is divided into Normal group, UVA group, the SF+UVA group of variable concentrations and positive controls.Experiment knot
According to the content of ROS and NO in test kit operating instruction detection cell culture fluid after bundle.
Result: high sulphation Sargassum polysaccharides to the clearance test result of HaCaT cell ROS as in figure 2 it is shown, cell passes through
Producing substantial amounts of ROS in cell culture fluid after UVA irradiation, the clearance rate of normal cell ROS is set as 100%, model group is thin
The clearance rate of born of the same parents ROS only has 43%, and each SF process group can significantly increase the clearance rate of ROS, wherein high dose group effect
Preferably, the clearance rate of its ROS, up to 90%, is better than positive control Vc group.High sulphation Sargassum polysaccharides is to HaCaT cell culture fluid
Middle NO affects result of the test as shown in Figure 3, and NO is signaling molecule important in body, plays most important in inflammatory reaction
Effect, cell produces substantial amounts of NO in cell culture fluid after UVA irradiates, and in model group cell culture fluid, the content of NO is about
For Normocellular 2 times, each SF process group cell all can the content of NO, Qi Zhonggao in a certain degree of reduction cell culture fluid
Concentration group effect is best, and the content of its NO is close to normal level.Show that sample SF has good antioxidation and antiinflammatory action.
The invention discloses the application in preparation Post-basking repairing mask of a kind of high Sulfated Sargassum polysaccharides.Face of the present invention
The raw material that film uses is natural material, nontoxic, non-stimulated, have no side effect；High Sulfated Sargassum polysaccharides has preferably suction
Wet moisturizing, antioxidation and antiinflammatory action, can be that skin provides adequate water, and can form lock moisture film holding moisture, simultaneously
Can calm skin tired, coarse, remove the ultra-oxygen anion free radical in skin, alleviate scytitis.This facial film can be answered
It is exposed to the sun for the sun nursing of rear face skin.It is more than the description to the embodiment of the present invention, by the disclosed embodiments
Described above, make professional and technical personnel in the field be capable of or use the present invention.Multiple amendment to these embodiments is right
Will be apparent from for those skilled in the art, generic principles defined herein can be without departing from this
In the case of bright spirit or scope, realize in other embodiments.Therefore, the present invention is not intended to be limited to shown in this article
These embodiments, and it is to fit to the widest scope consistent with principles disclosed herein and features of novelty.
Claims (8)
1. one kind high Sulfated Sargassum polysaccharides application in Post-basking repairing mask.
Application the most according to claim 1, it is characterised in that the Sulfated Sargassum polysaccharides of described height can become as activity
Part is applied in Post-basking repairing mask.
Application the most according to claim 2, it is characterised in that the Sulfated Sargassum polysaccharides of described height is in Post-basking repairing mask
In application refer to that this facial film can apply to the nursing of the face skin after the sun is exposed to the sun.
Application the most according to claim 1 and 2, it is characterised in that the Sulfated Sargassum polysaccharides of described height refers to sulfate
Content is 20-45%, and molecular weight is 12,000-87, the Brown algae sulfated polysaccharide of 000Da.
5. according to the application described in claim 1,2,3 or 4, it is characterised in that prepared by the Sulfated Sargassum polysaccharides of described height
Post-basking repairing mask there is moisture-absorbing moisture-keeping, antioxidation and antiinflammatory action, to shine after skin have good repair.
6. according to the application described in claim 1,2 or 4, it is characterised in that described facial mask liquid includes following parts by weight raw material:
High Sulfated Sargassum polysaccharides 1-10 part, glycerol 5-15 part, olive oil 3-10 part, vitamin B5 1-5 part, deionized water 60-90
Part.
Application the most according to claim 6, it is characterised in that described facial film preparation method comprises the steps:
(1) the high Sulfated Sargassum polysaccharides solution of preparation mass concentration 1-8%；
(2) in solution, add the stirring of aequum vitamin B5, after dissolving, add aequum glycerol and olive oil stirring；
(3) paper membrane is put into immersion more than 1h in facial mask liquid, must have the facial film of after-sun function.
8. according to the arbitrary described application of claim 1-7, it is characterised in that: the Sulfated Sargassum polysaccharides of described height derives from
One in Phaeophyta Laminariales and Fucales Sargassum or two kinds.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111297738A (en) * | 2020-04-24 | 2020-06-19 | 山东大学 | Seaweed mask and preparation method and application thereof |
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| CN106038355B (en) | 2019-11-12 |
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