KR20000020728A - Skin whitening composition - Google Patents

Abstract

PURPOSE: A skin whitening composition is provided which improves liver spots or freckles, has an excellent whitening effect, and is stable to the skin. CONSTITUTION: The composition comprises one or more extracts selected from the group consisting of the extract of Rhizoma nardostachysis, the extract of Spore lygodii, and the extract of Cucurbit moschat a Duchesne in the amount of 0.0001-10.0 wt%, preferably 0.0005-10.0 wt%, more preferably 0.005-0.5 wt% by dry weight. The extracts are prepared by pulverizing Rhizoma nardostachysis, Spore lygodii or Cucurbit moschat a Duchesne; adding the pulverized matter to 5-20 volume of water, alcohols of C1-C4, ethyl acetate or acetone based on the dry weight of the pulverized matter; extracting it at 50-100°C for 1-5 hours; filtering it; repeating the extracting procedure; concentrating the residue; and lyophilizing or spray-drying it. The composition can be prepared in the form of ointments, lotions, beauty washes, packs or essences.

Description

Skin Whitening Composition

The present invention relates to a composition for blemishes, freckles, and skin whitening, which contains one extract selected from the group consisting of persimmon extract, haegeum sasa extract, and pumpkin extract, and more specifically, persimmon extract having an excellent melanin production inhibitory effect. The present invention relates to a pharmaceutical and cosmetic composition containing one species of extract selected from the group consisting of Haegeumsa extract and pumpkin extract.

It is everyone's constant desire to have white, fair skin. Human skin color is genetically determined by the concentration and distribution of melanin in the skin, but is also influenced by environmental or physiological conditions such as sun ultraviolet rays, fatigue and stress. Melanin is made through a non-enzymatic oxidation reaction after the action of tyrosine, an amino acid, tyrosinase, which is converted into dopa and dopaquinone. However, although the pathway by which melanin is made is known, it is still not clear what mechanism is responsible for inducing melanin synthesis, the previous step in which tyrosinase works.

Thus, conventionally, substances having inhibitory activity against tyrosinase such as hydroquinone, ascorbic acid, kojic acid, glutathione, and placenta extract have been used in ointments or cosmetics to improve skin whitening, blemishes, and freckles. However, hydroquinone is recognized for its whitening effect but its skin irritation is extremely limited and its usage is extremely limited. Has a characteristic unpleasant odor and has problems with percutaneous absorption. In addition, glycosides and derivatives thereof have a high polarity and thus have problems in being used as a component of cosmetics. On the other hand, placenta extract has a problem of insufficient whitening effect.

Therefore, the present inventors use natural mice using melanoma cells (B16 mouse melanoma cells) that can screen (screening) to a substance that inhibits melanin synthesis induction for the purpose of solving the problems of the conventional skin lightening agent. As a result of repeated studies to develop a new whitening agent for native plants and plants, the present invention was found to have a very strong melanin production inhibitory effect on the extracts of Gamsong-hyang, Haegeumsa and pumpkin extracts.

The present invention relates to a composition for blemishes, freckles, and skin whitening containing an extract selected from the group consisting of persimmon extract, haegeum sasa extract, and pumpkin extract.

Persimmon is a perennial herb, Latin name is Rhizoma Nardostachysis, grows in alpine grasslands or grassland trenches. It is harvested in spring and autumn and used caliber or root. Persimmon is a member of the family Valerianaceae. The plant of origin in Korea is Nardostachysis chinensis Batal. In addition to this, it is defined as Nardostachysis jatamansi in China. Persimmon scent has traditionally been used in various herbal medicines such as blotch pain, vomiting, anorexia, toothache, etc. In addition, researches on cosmetic compositions (see Japanese Patent Application Laid-Open No. 8-259423) and dermatological treatments (Japanese Patent Application Laid-open No. 5-246874) have been reported in connection with sweet odor. However, no skin whitening effect has been reported. According to the existing research on the identification of the sweet sensation component, compounds such as ethyl-β-D- glucopyranoside, Nardostachin, Kanshone, Nardorfuran and the like have been reported.

Haegeumsa is a perennial herbaceous herb, Latin name is Spora Lygodii, and is used in the autumn in the wild on the slopes of mountain slopes. Haegumsa belongs to the Schizaeaceae family, and the plant is defined as Lygodium japonica in both Korea and China. Haegumsa has traditionally been used as an herbal medicine for urinary tract infections, urethral stones, nephritis, and fever of infection (see Herbal Wood). In addition, research on hepatitis drugs (CN 1128163), fracture drugs (CN 1120950), syphilis, and prostatitis drugs (CN 1097606) has been reported. However, no skin whitening effect has been reported. According to the existing Haegumsa company identification studies, gibberellin A9 methyl ester, 12-α-hydroxygibberellin A9 and the like have been reported.

Pumpkin (Cucurbita moschata Duchesne) is a tropical American evergreen plant belonging to the Cucurbitaceae family and is widely grown. In China, it is called as "encyclopedia" and has been used as an herbal medicine for anti-inflammatory pain, detoxification insecticide, middle-aged fruit, postpartum limb edema, etc. (cf. In addition, studies related to pumpkin have been reported for health food such as fatigue recovery (Japanese Patent Laid-Open No. 57122765). However, no skin whitening effect has been reported. Previous studies on the identification of pumpkin showed that pumpkin pulp contains Trigonelline, Carotene, Citrulline, amino acids and sugars, and seeds such as Cucurbitine, vitamins (Vitamin A, Compounds such as B ₁ , B ₂ , C), carotene and fatty acids have been reported.

Persimmon scent, Haegeumsa and pumpkin extract used in the present invention can be prepared as follows.

Buy a medicinal herb, Gumsongsa, Haegeumsa and Zucchini, which are commercially available and refrigerated with 5 to 20 volumes of water, anhydrous or hydrous lower alcohols of 1 to 4 carbon atoms, ethyl acetate or acetone for the dry weight of the ground product Extract by heating to 50-100 ° C. for 1-5 hours in an extractor with Thereafter, the filtrate was filtered and the residue was further extracted one or more times in the same manner. The extracts were combined and concentrated under reduced pressure, followed by freeze drying or spray drying to obtain dry extracts.

The skin whitening agent composition of the present invention is 0.0001 to 10.0% by weight, preferably 0.0005 to 10.0% by weight, and more preferably, one or two or more extracts selected from the group consisting of persimmon extract, haegeum extract and pumpkin extract. Preferably 0.005 to 0.5% by weight. When the amount of use is less than 0.0001% by weight, no obvious effect can be expected, and when the amount is more than 10.0% by weight, no apparent effect increases with the increase of the content.

The composition according to the present invention may be prepared by external skin ointment, lotion, lotion, pack or essence according to a conventional method.

Persimmon extract, Haegeumsa extract and pumpkin extract prepared according to the present invention showed very strong melanin inhibitory effect in B16 mouse melanoma cells, the composition prepared by including one or two or more When applied to the skin showed a very good skin whitening effect without showing any side effects.

Hereinafter, the present invention will be described in detail with reference to Examples, but these Examples do not limit the technical scope of the present invention.

Example

Example 1

Purchasing a commercially available persimmon fragrance, 300 g of pulverized powder was put in 3 l of 80% methanol solution, boiled for 3 hours in a reflux extractor with a cooling condenser, filtered through a 300 mesh filter cloth, and the residue was filtered once. More extracted. Each extract was combined and filtered using Whatman No. 2 filter paper at room temperature to remove insoluble matters, concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser, and lyophilized to extract 45 g of dried persimmon extract with a dry weight of 45 g. Got it.

Example 2

300 g of a pulverized crushed product was purchased and ground in 3 L of purified water, and the same method as in Example 1 was carried out to obtain a dry weight of 53 g of sweet persimmon extract.

Example 3

300 g of the pulverized crushed product purchased from commercially available persimmon was put in 3 l of an 85% ethanol solution, and the same method as in Example 1 was carried out to obtain a dry weight 48 g persimmon extract.

Example 4

300 g of a pulverized crushed product was purchased and ground into 3 L of a 70% acetone solution, and the same method as in Example 1 was carried out to obtain a dry weight 43 g of persimmon extract.

Example 5

300 g of the pulverized crushed powder purchased from commercially available persimmon was put in 3 L of a 50% ethanol solution, and the same method as in Example 1 was carried out to obtain a dry weight 51 g persimmon extract.

Example 6

300 g of a pulverized pulverized product purchased from commercially available persimmon was put in 3 l of a 60% methanol solution, and the same method as in Example 1 was carried out to obtain a dry weight of 49 g persimmon extract.

Example 7

300 g of a pulverized crushed product purchased from commercially available persimmon was put in 3 L of saturated normal butanol solution, and the same method as in Example 1 was carried out to obtain a dry weight of 41 g persimmon extract.

Example 8

300 g of a pulverized powder obtained by purchasing a commercially available persimmon fragrance was put in 3 L of a chloroform solution, and the same method as in Example 1 was carried out to obtain a dry weight 42 g persimmon extract.

Example 9

300 g of the pulverized powder obtained by purchasing a commercially available Haegum Sasa was pulverized in the same manner as in Example 1 to obtain a dry weight 25g Haegum Sasa extract.

Example 10

300 g of the pulverized product obtained by purchasing and pulverizing a commercially available haegum sand was carried out in the same manner as in Example 2 to obtain a 27g dry weight haegum sand extract.

Example 11

300 g of a pulverized product obtained by purchasing a commercially available Haegum Sasa was pulverized in the same manner as in Example 3 to obtain a dry weight of 30g Haegum Sasa extract.

Example 12

300 g of the pulverized powder obtained by purchasing a commercially available haegum sand was pulverized in the same manner as in Example 4 to obtain a dry weight of 24g haegum sand extract.

Example 13

300 g of a pulverized product obtained by purchasing a commercially available haegum sand was pulverized in the same manner as in Example 5 to obtain a 28g dry weight haegum sand extract.

Example 14

300 g of the pulverized powder obtained by purchasing commercially available Haegum sand was pulverized in the same manner as in Example 6 to obtain a dry weight 25g Haegum sand extract.

Example 15

300 g of a pulverized product obtained by purchasing a commercially available Haegum Sasa was pulverized in the same manner as in Example 7 to obtain a dry weight 23g Haegum Sasa extract.

Example 16

300 g of the pulverized powder obtained by purchasing a commercially available haegum sand was pulverized in the same manner as in Example 8 to obtain a 27g dry weight haegum sand extract.

Example 17

A commercially available pumpkin was pulverized and 300 g of a pulverized product was performed in the same manner as in Example 1 to obtain a dry weight of 56 g of a pumpkin extract.

Example 18

300 g of a pulverized product obtained by purchasing commercially available pumpkin was pulverized in the same manner as in Example 2 to obtain a dry weight of 58 g of a pumpkin extract.

Example 19

A commercially available pumpkin was pulverized and pulverized 300 g was carried out in the same manner as in Example 3 to obtain a dry weight of 55g pumpkin extract.

Example 20

A commercially available pumpkin was pulverized and 300 g of the pulverized product was carried out in the same manner as in Example 4 to obtain a dry weight of 60 g of a pumpkin extract.

Example 21

A commercially available pumpkin was pulverized and pulverized 300 g was carried out in the same manner as in Example 5 to obtain a dry weight of 57g pumpkin extract.

Example 22

A commercially available pumpkin was pulverized and pulverized 300 g was carried out in the same manner as in Example 6 to obtain a dry weight 54g of pumpkin extract.

Example 23

A commercially available pumpkin was pulverized and 300 g of the pulverized product was carried out in the same manner as in Example 7 to obtain a dry weight of 52 g of a pumpkin extract.

Example 24

A commercially available pumpkin was pulverized and 300 g of the pulverized product was carried out in the same manner as in Example 8 to obtain a dry weight of 58 g of a pumpkin extract.

Experimental Example 1

Each extract obtained according to Examples 1 to 24 was added to the culture solution of B16 mouse melanoma cells to test the whitening effect at the cellular level (Lotan R., Lotan D. Cancer Res. 40: 3345-3350, 1980). Each of the extracts obtained according to Examples 1 to 24 were added to a culture medium of B-16 melanoma cells with hydroquinone aqueous solution, each of which was brought to a final concentration of 0.005%, and then cultured for 3 days. After centrifugation, the melanin was extracted. Add 1 ml of sodium hydroxide solution (1N concentration), boil for 10 minutes to dissolve melanin, and measure the absorbance at 400 nanometer (nm) using a spectrophotometer to measure the amount of melanin produced per cell (10 ⁶ cells). ) Absorbance, and the results of calculating the melanin production relative to the control group as inhibition rate (%) are shown in Table 1 below.

From the results of Table 1, considering that each of the samples obtained according to Examples 1 to 24 is an extract that is not purified at all, compared to the previously known whitening substance hydroquinone, very potent melanin production inhibitory ability against the cultured melanoma cells of rats It can be seen that.

Inhibitory Effects of Melon Persimmon Extract, Haegeumsa Extract, and Pumpkin Extract on the Cell Level sample Melanin production % Inhibition Control group (no addition) 0.044 ± 0.004 - Example 1 (final concentration 0.005%) 0.026 ± 0.002 41% Example 2 (final concentration 0.005%) 0.029 ± 0.003 34% Example 3 (final concentration 0.005%) 0.027 ± 0.003 39% Example 4 (final concentration 0.005%) 0.028 ± 0.002 36% Example 5 (final concentration 0.005%) 0.030 ± 0.001 32% Example 6 (final concentration 0.005%) 0.029 ± 0.001 34% Example 7 (final concentration 0.005%) 0.024 ± 0.004 45% Example 8 (final concentration 0.005%) 0.023 ± 0.001 48% Example 9 (final concentration 0.005%) 0.025 ± 0.003 43% Example 10 (final concentration 0.005%) 0.026 ± 0.002 41% Example 11 (final concentration 0.005%) 0.025 ± 0.002 43% Example 12 (final concentration 0.005%) 0.027 ± 0.003 39% Example 13 (final concentration 0.005%) 0.020 ± 0.002 55% Example 14 (final concentration 0.005%) 0.020 ± 0.004 55% Example 15 (final concentration 0.005%) 0.021 ± 0.002 52% Example 16 (final concentration 0.005%) 0.022 ± 0.001 50% Example 17 (final concentration 0.005%) 0.027 ± 0.001 39% Example 18 (final concentration 0.005%) 0.030 ± 0.002 32% Example 19 (final concentration 0.005%) 0.027 ± 0.002 39% Example 20 (final concentration 0.005%) 0.028 ± 0.001 36% Example 21 (final concentration 0.005%) 0.027 ± 0.002 39% Example 22 (final concentration 0.005%) 0.027 ± 0.003 39% Example 23 (final concentration 0.005%) 0.025 ± 0.003 43% Example 24 (final concentration 0.005%) 0.024 ± 0.001 45% Hydroquinone (final concentration 0.0001%) 0.025 ± 0.002 43%

* Number of iterations = 3

Experimental Example 2

The melanoma production inhibitory ability of the rat melanoma cells cultured in the same manner as in Experimental Example 1 was investigated for the extracts of Examples 1, 9 and 17, and the results are shown in Table 2 below.

From the results of Table 2, it can be seen that the extract persimmon extract, Haegeumsa extract and pumpkin extract has a very strong melanin production inhibitory ability against the cultured melanoma cells. Hydroquinone has a strong melanin production inhibitory ability at low concentrations, but the cytotoxicity is so severe that the experiment can not be carried out at a concentration of 0.0001% or more, whereas the extracts according to the present invention can be used without showing cytotoxicity even at a concentration of 0.05% or more It has a higher melanin production inhibitory effect than hydroquinone. In addition, considering that the extract of persimmon scent, Haegeumsa extract and pumpkin extract has a very strong melanin production inhibitory ability considering that it is not a single substance but a mixture, it can be very useful for improving blemishes and freckles or skin whitening.

Inhibitory Effects of Melanin Production at Cell Level sample Application concentration (%) Melanin production % Inhibition Control group (no addition) - 0.044 ± 0.004 - Example 1 Final concentration (0.0005%) 0.041 ± 0.002 7% Final concentration (0.005%) 0.029 ± 0.002 34% Final concentration (0.05%) 0.020 ± 0.002 55% Final concentration (0.5%) 0.015 ± 0.001 66% Example 9 Final concentration (0.0005%) 0.040 ± 0.002 9% Final concentration (0.005%) 0.025 ± 0.003 43% Final concentration (0.05%) 0.018 ± 0.002 59% Final concentration (0.5%) 0.014 ± 0.001 68% Example 17 Final concentration (0.0005%) 0.041 ± 0.002 7% Final concentration (0.005%) 0.027 ± 0.001 39% Final concentration (0.05%) 0.022 ± 0.001 50% Final concentration (0.5%) 0.019 ± 0.002 57% Hydroquinone (0.0001%) 0.025 ± 0.002 43%

Iterations = 3

Prescription examples of cosmetics containing persimmon extract, Haegeumsa extract or pumpkin extract is shown in the following preparation and comparative examples.

Preparation Examples 1 to 3 and Comparative Example 1

Using an ingredient of Table 3, an external skin ointment containing persimmon extract, haegeum extract or pumpkin extract was prepared according to a conventional manufacturing method. As persimmon extract, Haegeumsa extract and pumpkin extract were used in Example 5, 13 and 21, respectively.

(100 wt% total) Composition Production Example Comparative Example 1 One 2 3 Extract Persimmon Scent Extract Hazeumsa Extract Pumpkin Extract-Diethyl Sebacate-Lead-Polyoxyethylene Oleyl Ether Phosphate-Sodium Benzoate-Vaseline 10--856 Quantity remaining Remaining balance Remaining balance --- 856 Quantity remaining

Preparation Examples 4 to 6 and Comparative Example 2

Using a component of the following Table 4 to prepare a cream cosmetic composition containing a persimmon extract, Haegeumsa extract or pumpkin extract according to a conventional cream cosmetic preparation method. As persimmon extract, Haegeumsa extract and pumpkin extract were used in Example 5, 13 and 21, respectively.

(100 wt% total) Composition Production Example Comparative Example 2 4 5 6 -Extract Persimmon Extract-Haze extract-Pumpkin Extract-Stearic Acid-Cetanol-Potassium Hydroxide-Glycerine-Propylene Glycol-Preservative-Perfume-Purified Water 1.0--15.01.00.75.03.0 -1.0 -15.01.00.75.03.0 --1.015.01.00.75.03.0 --- 15.01.00.75.03.0

Preparation Examples 7 to 9 and Comparative Example 3

Using the components of Table 5 to prepare a flexible cosmetic composition containing a persimmon extract, Haegeumsa extract or pumpkin extract according to a conventional cosmetic preparation method. As persimmon extract, Haegeumsa extract and pumpkin extract were used in Example 5, 13 and 21, respectively.

(100 wt% total) Composition Production Example Comparative Example 3 7 8 9 Extract Extract Persimmon Extract Haze extract Extract Pumpkin Extract-Ethanol-Polylauric acid Polyoxyethylene sorbitan-Paraoxybenzoic acid Methyl-Glycerin-1, 3-butylene glycol-Perfume-Color-Purified water 0.2--10.01.00.25.06.0 -0.2 -10.01.00.25.06.0 --0.210.01.00.25.06.0 --- 10.01.00.25.06.0 Balance

Preparation Examples 10 to 12 and Comparative Example 4

Using the components of Table 6 to prepare a nutrient cosmetic composition containing a persimmon extract, Haegeumsa extract or pumpkin extract according to a conventional cosmetic preparation method. As persimmon extract, Haegeumsa extract and pumpkin extract were used in Example 5, 13 and 21, respectively.

(100 wt% total) Composition Production Example Comparative Example 4 7 8 9 -Sex extract-Scented extract Haze extract-pumpkin extract-Waselin-Sesquioleate-Sorbitan-Polyoxyethylene oleyl ethyl-Paraoxybenzoic acid methyl-Propylene glycol-Ethanol-Carboxy vinyl polymer-Potassium hydroxide-Pigment-Flavor-Purified water 0.1--2.00.81.2 Quantity 5.03.218.00.1 -0.1-2.00.81.2 Accurate 5.03.218.00.1 Qualification --0.12.00.81.2 appropriate 5.03.218.00.1 --- 2.00.81.2 Suitable 5.03.218.00.1

Preparation Examples 13 to 15 and Comparative Example 5

To prepare a pack composition containing the persimmon extract, Haegeumsa extract or pumpkin extract according to the conventional pack manufacturing method using the components of Table 7. As persimmon extract, Haegeumsa extract and pumpkin extract were used in Example 5, 13 and 21, respectively.

(100 wt% total) Composition Production Example Comparative Example 5 13 14 15 Extract Extract Persimmon Extract Hazeumsa Extract Pumpkin Extract-Glycerin-Propylene Glycol-Polyvinyl Alcohol-Ethanol-Polyoxyethylene Oleylethyl-Paraoxybenzoic Acid-Flavor-Pigment-Purified Water 0.5--5.04.015.08.01.00.2Capacity -0.5-5.04.015.08.01.00.2Capacity --0.55.04.015.08.01.00.2Quality balance --- 5.04.015.08.01.00.2Capacity

Preparation Examples 16 to 18 and Comparative Example 6

Using the components of Table 8 to prepare an essence composition containing a persimmon extract, Haegeumsa extract or pumpkin extract according to a conventional essence manufacturing method. As persimmon extract, Haegeumsa extract and pumpkin extract were used in Example 5, 13 and 21, respectively.

(100 wt% total) Composition Production Example Comparative Example 6 16 17 18 -Sex extract-Hyanggum extractHaeumsa extract pumpkin extract-propylene glycol-glycerin-sodium hyaluronate aqueous solution (1%) 5--10.010.05.05.01.00.1 -5-10.010.05.05.01.00.1 --510.010.05.05.01.00.1Capacity balance --- 10.010.05.05.01.00.1Capacity balance

Experimental Example 3

Twenty healthy men and women were selected to attach aluminum foil in two rows of six holes with a diameter of 7 mm in the lower part of both arms, and 60 mJ / ㎠ light intensity was obtained using ORIEL solar simulator 1000W at a distance of 10 cm from the arm. Investigate. The irradiation site was well cleaned with 70% ethanol aqueous solution before irradiation. Samples containing persimmon extract, Haegeumsa extract or pumpkin extract prepared according to Preparation Examples 1 to 18 and Comparative Examples 1 to 6, twice a day from 3 days before irradiation to 3 weeks after irradiation, and the base containing no extract thereof Were applied in pairs on the same line (in the case of pack formulations of Preparation Examples 13, 14, 15 and Comparative Example 5, they were removed 15 minutes after application). For each, the pigmentation degree of the preparation example and the comparative example were visually judged, and the degree of suppression of the pigmentation of the preparation example compared to the comparative example was evaluated in three stages of "clear effect, effect, no difference", and the result was evaluated. It is shown in Table 9 below.

Inhibition of Pigmentation of Ointments and Cosmetics Containing Persimmon Extract, Haegeumsa Extract or Pumpkin Extract Experimental substance Distinct Effects (persons) Effective (persons) No difference (persons) Preparation Example 1 6 8 6 Preparation Example 2 5 9 6 Preparation Example 3 7 7 6 Preparation Example 4 3 8 9 Preparation Example 5 4 8 8 Preparation Example 6 4 7 9 Preparation Example 7 2 10 8 Preparation Example 8 2 10 8 Preparation Example 9 2 10 8 Preparation Example 10 4 7 9 Preparation Example 11 2 10 8 Preparation Example 12 3 8 9 Preparation Example 13 2 10 8 Preparation Example 14 One 9 10 Preparation Example 15 One 9 10 Preparation Example 16 6 7 7 Preparation Example 17 6 8 6 Preparation Example 18 5 9 6

As can be seen from Table 9, the whitening ointment and cosmetics containing the persimmon extract, Haegeumsa extract or pumpkin extract prepared according to Preparation Examples 1 to 18 showed a whitening effect on at least 10 of 20 subjects. In particular, in the comparative experiments of Preparation Examples 1, 2, and 3 and Comparative Example 1 to which a large amount of the extract of Persimmon Extract, Haegeumsa extract or Pumpkin Extract was applied, 70% showed pigmentation inhibitory effect and did not show any side effects in the skin. It can be seen that the extract, haegeumsa extract or pumpkin extract is a safe and very effective blemish, freckles improver or skin lightening agent.

As described above, the composition containing one species of the extract selected from the group consisting of persimmon extract, haegeum sasa extract and pumpkin extract according to the present invention is a safe and very excellent blemishes, freckles improver and skin lightening composition.

Claims (3)

Skin whitening agent composition containing one extract selected from the group consisting of persimmon extract, Haegeumsa extract and pumpkin extract as an active ingredient. The composition of claim 1 containing 0.0001 to 10.0% by weight of the extract in dry weight. The composition of claim 1, wherein the extract is obtained by extracting persimmon scent, haegeum saccharine or amber with water, lower alcohols having 1 to 4 carbon atoms, ethyl acetate or acetone, and distilling the extract under reduced pressure followed by lyophilization or spray drying. .