KR102014374B1 - Functional rice and manufacturing method thereof - Google Patents

Abstract

The present invention relates to arrowhead healthy rice and a method of manufacturing the same, and more specifically, the characteristics of sodium cypressoacetate containing arrowwood to increase insulin secretion by supplying distilled water of arrowwood (hamle) extract to the medium for shiitake mushroom production. Cultured shiitake mushrooms (arrow shiitake mushrooms), and steamed mixed cultured arrow shiitake mushrooms with dilution water of arrowwood and oak mistletoe extract to produce healthy juice (hereinafter referred to as 'arrow shiitake'), the arrow By adding the gourd juice to the washed rice in the rice cooker instead of water to make healthy rice containing arrow gourd (hereinafter referred to as 'arrow arrow healthy rice'), the white rice (white rice), a staple food of Koreans, contains arrow juice Avoiding rice, especially through taste, aroma, and color (chocolate color), while promoting metabolism, enhancing immunity, preventing various adult diseases, and improving diet. Relates to the health of rice and its manufacturing method that allows you to derive the intake by infants are having interesting.

Description

Arrow Go Healthy Rice and Manufacturing Method {FUNCTIONAL RICE AND MANUFACTURING METHOD THEREOF}

The present invention relates to arrowhead healthy rice and a method of manufacturing the same, and more specifically, the characteristics of sodium cypressoacetate containing arrowwood to increase insulin secretion by supplying distilled water of arrowwood (hamle) extract to the medium for shiitake mushroom production. Cultured shiitake mushrooms (arrow shiitake mushrooms), and steamed mixed cultured arrow shiitake mushrooms with dilution water of arrowwood and oak mistletoe extract to produce healthy juice (hereinafter referred to as 'arrow shiitake'), the arrow By adding the gourd juice to the washed rice in the rice cooker instead of water to make healthy rice containing arrow gourd (hereinafter referred to as 'arrow arrow healthy rice'), the white rice (white rice), a staple food of Koreans, contains arrow juice Avoiding rice, especially through taste, aroma, and color (chocolate color), while promoting metabolism, enhancing immunity, preventing various adult diseases, and improving diet. Relates to the health of rice and its manufacturing method that allows you to derive the intake by infants are having interesting.

Koreans' diet is made of rice as a staple, so in most households it is common to cook rice with white rice and consume it. White rice is soft, so it is unlikely to cause digestive problems, has a good taste, and does not spoil. However, since most of the nutrients are shaved and only a high proportion of carbohydrates may cause nutritional imbalance. Extremely carbohydrate poisoning can also be ruled out. In addition, white rice is low in fiber, so the risk of constipation increases.

Therefore, as an extension of the well-being fever in the past, in recent years, as the health information becomes a very important factor for the majority of people, the interest is increasing, and each family gradually increases the consumption of various grains rather than white rice. There is a situation.

In terms of mixed grains, cereals contain nutrients evenly in whole grains without skins. This is because most of the important nutrients, antioxidants and minerals are contained in the shell and shell of the grain. Therefore, the intake of mixed grains can help to prevent various adult diseases and constitution. However, grains without shells are rough, difficult to eat, tasteless, and indigestible for a variety of reasons.

Patent No. 10-1027413 'Mixed grain composition for improving and preventing diabetes' is to include a combination of giant embryonic rice, giant embryonic brown rice, waxy barley, black rice, maize rice, eastern soybeans, Seomoktae and kidney beans to prevent various adult diseases such as diabetes. It is characterized by that.

However, even with the registered patent, it is difficult to solve the above-described various problems such as indigestion.

Therefore, the inventors of the present invention contain arrow shiitake juice in white rice (white rice) to induce interest through taste, aroma and color, and at the same time, arrow gogi healthy rice and a method for manufacturing the same to prevent various adult diseases It is intended to disclose.

In order to solve the problems of the prior art,

The present invention can promote diabetes, indigestion prevention, anti-cancer effect, prevention of hypertension, active metabolism and immunity through health rice containing arrow juice that additionally expresses the characteristics of arrowwood extract along with the characteristics of shiitake mushrooms. The purpose is to provide an arrow and healthy rice and a method of manufacturing the same.

In addition, the present invention arrow arrow healthy rice and its manufacturing method to prevent the hassle of ingestion by being directly contained in the white rice, which is a staple of Koreans, without separately ingesting the arrow juice of the above-mentioned healthy juice or health drink The purpose is to provide.

In addition, the present invention provides an arrow-go healthy rice and a method of manufacturing the same through the chocolate-colored healthy rice containing arrow juices, in particular to induce interest in children avoiding the intake of rice. Its purpose is to.

In addition, the present invention arrows and healthy rice to increase the secretion of arrow shiitake mushrooms, distilled water of purified high-quality extract dilution of arrowwood and oak mistletoe which is effective in immunity and to promote additional pharmacological effect The purpose is to provide a manufacturing method.

The present invention can be implemented by an embodiment having the following configuration to achieve the above object.

According to an embodiment of the present invention, the arrowhead healthy rice and its manufacturing method according to the present invention comprises the steps of providing a distilled water extract of arrowwood using a needle bar for the medium in oak sawdust medium;

Drying and cutting the cultured shiitake mushrooms (arrow shiitake mushrooms) by absorbing the dilution water of the arrow-tree extract;

Bubble washing the dried and cut arrow shiitake mushrooms with distilled water of arrowwood extract;

Mixing and concentrating purified water and licorice together with an arrow shiitake in a heat concentrator;

Extracting and filtering the arrow shiitake extract;

Mixing and steaming the extract dilution water of arrowwood and oak mistletoe to the arrow shiitake mushroom extract;

Steaming the arrow juice prepared by the above step at room temperature;

The arrow juice is administered to the white rice washed in the cooker; characterized in that it comprises a.

According to another embodiment of the present invention, the amount of arrow juice is administered to the arrowhead healthy rice and the white rice of the manufacturing method according to the present invention is characterized in that 200 ~ 330 ml compared to 90 ~ 100g of rice.

According to another embodiment of the present invention, the arrow gourd juice prepared by arrow gogi healthy rice and the manufacturing method according to the present invention 35 ~ 50% by weight of the arrow shiitake mushroom extract, dilution 13 ~ of the arrowwood and oak mistletoe It is characterized by consisting of 20% by weight, licorice 0.1-0.2% by weight, and purified water.

According to another embodiment of the present invention, the arrowwood dilution water provided in oak sawdust medium through the arrowwood extract dilution water providing step of the arrowgo healthy rice manufacturing method according to the present invention is the arrow washed after being collected Drying the leaves and branches of the tree; Cutting the dried leaves and branches into a predetermined size; Mixing the cut leaves and branches with an amount of water and concentrating with a heat concentrator; Cooling the arrow extract extracted through the concentration step; Storing the cooled arrowwood extract under 1.5 ° C ~ 3.5 ° C; characterized in that it comprises a.

According to another embodiment of the present invention, the arrowwood extract dilution water provided in oak sawdust medium through the arrowwood extract dilution water providing step of the arrowhead health rice and its manufacturing method according to the present invention is 100 to dilution water 20ℓ 100 It is characterized by consisting of ~ 200g arrowwood extract.

According to another embodiment of the present invention, the arrowwood extract dilution water provided in oak sawdust medium through the arrowwood extract dilution water providing step of the arrowhead healthy rice and its manufacturing method according to the present invention is supplied to the needle bar for the medium Even after the cycle is characterized in that the medium is sprayed once every 7 days on average.

According to another embodiment of the present invention, the step of drying and cutting the arrow gogi mushrooms of the arrow gogi healthy rice and the manufacturing method according to the present invention after drying the arrow shiitake mushrooms for 2 to 4 hours at 25 ° C. Shiitake mushrooms are cut into three equal portions, characterized in that the low-temperature aging under 1.5 ~ 3.5 ° C.

The present invention has the following effects by the above configuration.

The present invention can promote diabetes, indigestion prevention, anti-cancer effect, prevention of hypertension, active metabolism and immunity through health rice containing arrow juice that additionally expresses the characteristics of arrowwood extract along with the characteristics of shiitake mushrooms. To have the effect.

In addition, the present invention has the effect of preventing the hassle of intake to be directly contained in the white rice, which is a staple food of Koreans without separately ingesting the arrow juice of the above-mentioned healthy juice or health beverage.

In addition, the present invention is derived through the chocolate-colored healthy rice containing the arrow juice, in particular to the children to avoid the intake of rice to induce an interest to induce natural intake.

In addition, the present invention exhibits the effect of increasing the secretion of arrow shiitake mushrooms, distilled water of purified high-quality extract dilution of arrowwood and oak mistletoe which is effective in immunity to achieve additional pharmacological effect.

1 is a block diagram of a method for producing arrow juice according to an embodiment of the present invention;
2 is a block diagram of a step of mixing and steaming the (advanced) extract dilution water of arrowwood and oak mistletoe according to FIG. 1;
Figure 3 is a view showing the results of immunohistochemical activity of mouse spleen cells of arrow juice contained in arrowhead healthy rice according to an embodiment of the present invention;
Figure 4 is a view showing the blood sugar strengthening effect of the arrow juice contained in arrowhead healthy rice according to an embodiment of the present invention;
5 is a view showing the effect of strengthening the exercise performance of the arrow contained in a healthy rice in accordance with an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the accompanying drawings. Embodiments of the invention may be modified in various forms, the scope of the invention should not be construed as limited to the following examples, but should be construed based on the matter described in the claims. In addition, this embodiment is merely provided as a reference to more fully explain the present invention to those skilled in the art.

This will be described in detail with respect to the arrowhead health rice and its manufacturing method according to an embodiment of the present invention below.

The present invention relates to arrowhead healthy rice and a method of manufacturing the same, and more specifically, the characteristics of sodium cypressoacetate containing arrowwood to increase insulin secretion by supplying distilled water of arrowwood (hamle) extract to the medium for shiitake mushroom production. Cultured shiitake mushrooms (arrow shiitake mushrooms), and steamed mixed cultured arrow shiitake mushrooms with dilution water of arrowwood and oak mistletoe extract to produce healthy juice (hereinafter referred to as 'arrow shiitake'), the arrow By adding the gourd juice to the washed rice in the rice cooker instead of water to make healthy rice containing arrow gourd (hereinafter referred to as 'arrow arrow healthy rice'), the white rice (white rice), a staple food of Koreans, contains arrow juice Avoiding rice, especially through taste, aroma, and color (chocolate color), while promoting metabolism, enhancing immunity, preventing various adult diseases, and improving diet. Relates to the health of rice and its manufacturing method that allows you to derive the intake by infants are having interesting.

Hereinafter, a method for producing arrow gogi healthy rice will be described in detail. First, after describing in detail the manufacturing method of the 'arrow arrowhead juice' contained in the rice, it will be described for the 'arrow arrow healthy rice' manufacturing method through the arrow arrow juice prepared.

1 is a block diagram related to the arrow juice production method according to an embodiment of the present invention.

1, the arrow juice according to an embodiment of the present invention,

1) providing a distilled water of arrowwood (hamberry) (intermediate) extract using a needle bar for the medium on oak sawdust medium;

2) drying and cutting the cultured shiitake mushrooms (hereinafter referred to as' arrow shiitake mushrooms) by absorbing the dilution water of the arrow-tree extract;

3) bubble washing the dried and cut arrow shiitake mushrooms with dilution water of the arrowwood (intermediate) extract;

4) mixing and concentrating purified water and licorice together with an arrow shiitake in a heat concentrator;

5) extracting and filtering the arrow shiitake mushroom extract;

6) mixing and steaming the (advanced) extract dilution water of arrowwood and oak mistletoe in the arrow shiitake mushroom extract;

And 7) packing and steaming the arrow juice in a pack after the step 6).

However, as will be described in detail below, the step of packaging in a pack in step 7) corresponds to an optional step, it should be noted that the process is not necessarily included in the scope of the present invention.

Hereinafter, to describe in detail the individual process of the arrow juice according to an embodiment of the present invention.

Hereinafter, 1) providing a distilled water of the saplings (arch) intermediate extract using a needle bar for the medium to oak sawdust medium; it will be described in detail.

Generally, the main ingredient of shiitake mushroom medium is oak sawdust because the medium using oak sawdust is good for the quality and yield of the mushroom. Among them, cultivation with oak sawdust is most preferable because the fresh and thick flesh is generated, but the scope of the present invention is not limited thereto.

In addition, the oak sawdust particles are less than 1 ~ 2 mm of air permeability in the medium and the surface area is increased, the growth rate of shiitake bacteria is slow, and if the particle size is 3 mm or more, the moisture may not be absorbed quickly when the medium is prepared. Suitable particles of sawdust used in the production is preferably used in a ratio of 1 to 2 mm and 3 mm or more, respectively. However, the scope of the present invention is not limited thereto.

Here, shiitake mushrooms are mushrooms of Pleurotus edodes and shiitake mushrooms, which contain eridadamine to lower blood cholesterol levels and contain ergosterol, a precursor of vitamin D as well as vitamins B1 and B2. In addition, the shiitake mushroom is an anti-cancer food that is rich in various amino acids and contains beta glucan, which is good for the body, to increase human immunity.

Step 1) provides the distilled water of arrowwood intermediate extract on the oak sawdust medium prepared as described above, hereinafter will be described in detail with respect to the arrowwood intermediate extract manufacturing step.

The arrowwood intermediate extract manufacturing step comprises the steps of drying the leaves and branches of the arrowwood washed after being collected; Cutting the dried leaves and branches into a predetermined size; Mixing the cut leaves and branches with an amount of water and concentrating with a heat concentrator; Concentrating; cooling the extracted extract; It comprises; storing the cooled extract at 1.5 ° C ~ 3.5 ° C.

The arrow tree is called a migraine because of the unusual appearance of the wings attached to the stem, it is used in the treatment of diabetes mellitus because it is confirmed that it contains sodium succinate to lower blood sugar and increase insulin secretion. In addition, it has the efficacy of suffocation of gut blood, arteriosclerosis, thrombosis, postpartum abdominal pain, dysmenorrhea, etc., folk remedies are also used for cancer treatment by picking leaves and branches.

The arrowwood intermediate extract prepared as described above is mixed in dilution water, for example, it is preferable to mix about 100-200 g of the arrowwood intermediate extract in dilution water 20L. This completes the arrowwood intermediate extract dilution water. If the mixed amount of the arrow extract is less than 100 g there is a fear that the inherent efficacy of the arrow tree may be lowered, when the amount exceeds 200 g may cause a problem that the cost effectiveness is insufficient.

Subsequently, the arrowwood intermediate extract dilution water is provided to the oak sawdust medium using a medium rod. For example, it is desirable to provide 1.5-2 L of intermediate extract dilution water. Under the numerical range as described above, the cost effectiveness of the arrow tree can be maximized.

In addition, even after the dilution water is supplied to the medium needles, the intermediate extract dilution water is periodically sprayed on the medium, and preferably, sprayed about once every 7 days. As a rule, it is more preferable to provide the medicines once every ten days after the occurrence of arrow shiitake mushrooms, and then slowly slow the cycle and then provide them once every 10 days after the occurrence of arrow shiitake mushrooms. Therefore, the arrowwood extract can be absorbed from the occurrence of arrow shiitake mushrooms, and at the same time, the intermediate extract dilution water is periodically provided even after the occurrence of arrow shiitake mushrooms so that the active ingredients of the arrowwood extracts penetrate the shiitake mushroom tissues and nourish them. There is an advantage to allowing this to be improved. However, it should be noted that the scope of the present invention is not limited by the above examples.

Therefore, 1) shiitake mushroom containing arrowwood extract can be cultured through the step of providing a dilution water of arrowwood (hamberry) intermediate extract using a needle bar for the culture on oak sawdust medium. Therefore, by absorbing the arrowwood extract from the occurrence of shiitake mushroom, not only the efficacy of the shiitake mushroom itself, but also to exhibit some of the efficacy of the arrowwood. In other words, 'arrow shiitake mushroom' is generated.

Hereinafter, 2) drying and cutting the arrow shiitake mushrooms to a certain size; will be described in detail.

First, look at the drying step, the arrow shiitake mushrooms are dried in the sun. The mycelial growth range is 5 to 32 ° C but the optimum temperature is 25 ° C. In addition, ergosterine contained in shiitake mushrooms has the property of changing to vitamin D when exposed to sunlight. Therefore, it is preferable to allow vitamin D to be contained in the shiitake mushroom through the drying step. For example, it is preferable to dry the arrow shiitake mushrooms produced at 25 ° C. for 2 to 4 hours, the vitamin D content may be insufficient when dried for less than 2 hours, and the effect may be higher than the drying time when dried for more than 4 hours. It may not be big.

After that, the arrow shiitake mushrooms are cut and aged at low temperature under 1.5 to 3.5 ° C. For example, by cutting the arrow shiitake mushrooms 3 to 4 equal parts to low temperature aging. It can prevent the deterioration or decay of the arrow shiitake mushrooms at low temperature aging. In addition, the low temperature drying immediately after high temperature drying can maximize the activity of shiitake mushrooms. However, it should be noted that the above-mentioned low temperature aging temperature and cut size of arrow shiitake mushrooms are exemplary, and the scope of the present invention is not limited to the above examples.

Hereinafter, 3) bubble washing the dried and cut arrow shiitake mushrooms with dilution water of the arrowwood extract; will be described in detail.

In general, bubbles staying in water compress themselves by external pressure, such as a balloon pushed into the water, and burst under water when pressure is applied. When the bubble bursts by the self-pressing effect, for example, a shock wave of instantaneous high temperature and high pressure is generated, and it is possible to sterilize harmful microorganisms. That is, to enable sterilization. In addition, it is generally known to exhibit a fermentation promoting effect upon bubble washing. In addition, bubble washing of the arrow shiitake mushroom according to the present invention as the intermediate arrow extract water, so that the dilution water of the arrow tree extract can be supplied and absorbed even after the generation of the arrow shiitake mushroom.

Hereinafter, 4) the step of mixing and concentrating the purified water and licorice in a heat concentrator with arrow shiitake mushroom; will be described in detail.

First, the bubble washed arrow shiitake mushrooms are mixed and concentrated in a heat concentrator with purified water and licorice. For example, it is heated at high temperature for 3 to 5 hours under 100 to 120 ° C. At this time, the amount is preferably 25 to 35 g of licorice with respect to about 12 kg of purified water. If the added amount of licorice is less than 25g pharmacological action may be lowered by the addition amount of licorice, pharmacological action is improved if it is more than 35g because the taste of arrow juice can be altered due to the excess amount. In general, licorice is rich in flavonoid-based substances, and has the effect of eliminating free radicals, improving liver function, suppressing allergies, relieving stress, and sore throat and cough. In addition, glycyrrhizin degradation products of licorice have an effect on acute and chronic hepatitis, and have been used as an AIDS treatment as an immune activity. In addition, the condition of the concentrator is preferably to be heated to a high temperature for 3 to 5 hours at 100 ~ 120 ° C at an input vacuum of 640 ~ 650 mmHg / 0.1 ~ 0.21kg / ㎠, the scope of the present invention is within the numerical range Note that it is not limited.

Thereafter, 5) extracting and filtering the arrow shiitake mushroom extract; and extracting and filtering the high temperature concentrated arrow shiitake mushroom extract.

Hereinafter, 6) mixing and steaming the advanced extract dilution water of the arrowwood and oak mistletoe in the arrow shiitake mushroom extract;

After the filtered arrow shiitake extract is transferred to a mixing packaging machine, purified high-quality extract waters of arrow and oak, which have undergone heating and concentrated steaming, are mixed and preferably aged for 30 to 40 minutes at 100 to 105 ° C. If the ripening time is less than 30 minutes, there is a possibility that an appropriate level of mixed ripening may not be possible.

Hereinafter, the step of packaging and steaming the arrow juice in a pack (Pack) after the steps 7) 6) will be described in detail. Preferably, sterilized vacuum extraction packaging to 1 pack / 100 ~ 110ml in a mixed packing machine maintained at 100 ° C or more. After the packaging is complete, the inside temperature of the baler is slowly lowered and steamed at room temperature for 24 to 30 hours. If the steaming time is less than 24 hours may cause a problem that the sufficient steaming is not achieved, if the steaming time is over 30 hours, the taste of the arrow juice can be altered. However, as described above, it should be noted that the arrow juice is not necessarily packed in a pack when manufacturing arrow gogi healthy rice according to the present invention.

Thereafter, the arrow juice is steamed for a predetermined time in a low temperature refrigerator under 1.5 ~ 3.5 ° C.

As a result, it is preferable that the finished product of the arrow juice contains 35 to 50% by weight of arrow shiitake mushroom extract, 13 to 20% by weight of advanced extract water, 0.1 to 0.2% by weight licorice. And other purified water. The effect of the numerical range can be confirmed through an experimental example to be described later in the present specification.

Figure 2 is a block diagram of the step of mixing and steaming (advanced) extract dilution water of arrowwood and oak mistletoe according to FIG.

Hereinafter, the (advanced) extract dilution water manufacturing method of the arrowwood and oak mistletoe mixed in step 6) will be described in detail. The advanced extracting water production method, the step of collecting the arrowwood and oak mistletoe and then separating the leaves, branches, stems and berries to dry; and cutting the dried material to a predetermined size; And mixing the cut materials and adding and mixing liquorice, root roots, plantain, goji mulberry and purified water; and concentrating the mixed material with a concentrator; and steaming and cooling the concentrated mixture for a predetermined time.

Looking at the drying step; the arrow and oak mistletoe harvested and washed and then separated by material to dry for 2 to 3 days. If the drying period is less than 2 days, the materials may not be sufficiently dried, and if it is more than 3 days, the materials may be excessively dried, resulting in material loss.

Looking at the cutting step; cutting the leaves, branches, stems, flowers, berries of arrowwood and oak mistletoe, preferably the step of cutting to a size 0.5 ~ 1.0cm. If the size of the cut material is less than 0.5cm, the effect of the active ingredient of the material at the time of concentration is insignificant compared to the energy consumption, and if it exceeds 1.0cm, there is a concern that the active material of the concentration is not sufficiently concentrated.

Looking at the addition mixing step; mixing the materials of the cut arrowwood and oak mistletoe by parts, and mixing licorice, root roots, plantain, Koji mulberry and purified water, preferably arrowwood and oak in purified water 1000ml It is preferable to add 10-15 g of mistletoe mixed material, 2-3 g of licorice, 3-4 g of dried root skin, 3-4 g of plantain, and 3-4 g of Koji mulberry.

The arrowwood is preferably mixed with 5 ~ 8g per 1000ml of purified water, and when the mixing amount is less than 5g, the functionality decreases due to the decrease in sodium citrate acetate content, and when the amount exceeds 8g, there is a possibility that the functionality of arrow juice is lowered due to the decrease in the mixing amount of other materials. . In addition, the mixing amount of the arrow tree is most suitable when considering the content of sodium citrate to mix the leaves 35 ~ 45% by weight, eggplant 35 ~ 45% by weight, flowers 10 ~ 15% by weight, fruit 10 ~ 15% by weight.

In addition, oak mistletoe contains oleanoic acid, saponin, amirin, etc., which increase anticancer effect and immunity as a main component, and inhibits cancer cells, and the mixing amount thereof is preferably 5-7 g in 1000 ml of purified portion. When the mixing amount is less than 5g, there is a fear that the metabolic function is lowered, and when the mixing amount is more than 7g, the effect is inferior.

The amount of the oak mistletoe is preferably 45 to 50% by weight, 40 to 45% by weight of stem, 5 to 8% by weight of flower, 5 to 8% by weight of fruit. This may fall.

In addition, the efficacy of licorice is replaced by the above description.

The roots of flaxseeds contain flavonoids, saponins, tannins, mucus, etc., the leaves contain flavonoids, fruits contain tannin mucus, etc., which have excellent pharmacological effects in treating gastrointestinal diseases, wounds, inflammations, etc. It is preferable to add 3-4 g with respect to 1000 ml of silver purified water. If the added amount of the roots of less than 3g pharmacological effect may be lowered due to the addition amount of the roots of the roots, the pharmacological effect may be improved when the amount of the roots added more than 4g, but the effect is weak compared to the amount added.

The plantain is rich in saponin, calcium, iron, minerals, proteins, vitamins, and fiber components, and has been used for long-term cold and lung function activity due to alkaline plants that have excellent effects on digestive absorption, blood purification, and immunity. As a herb widely used in respiratory diseases such as protection of throat, it is preferable to add 3 to 4 g based on 1000 ml of purified water. If the amount of plantain is less than 3g, the pharmacological effect may be lowered due to a decrease in the amount of plantain added. If the amount of plantain is more than 4g, the pharmacological effect may be improved, but the effect is weak compared to the amount added.

Unlike the common mulberry, Guji mulberry has a thorn on the stem of the tree and leaves are similar to persimmon leaves but thick and fluffy. When the mulberry leaves are removed, they are absorbed in white and thick liquid, rutin, morphin and vitamins B1, B2, C, etc. It contains a component and has a turbid effect on various cancers, chronic hepatitis, inflammation, neuralgia, etc., and the amount of the addition is preferably 3 to 4 g based on 1000 ml of purified water. If the addition amount of the Gujimi mulberry tree is less than 3g pharmacological effect may be lowered by the addition amount of Guji mulberry tree, the pharmacological effect may be improved when the addition amount of Guji mulberry is more than 4g, but the effect is weak compared to the addition amount.

As for the concentration step, as a step of heating and concentrating the mixed material by the concentrator, preferred concentrator conditions are 120 ± 5 minutes at 120 ± 1 ℃ at an input vacuum of 640 ~ 650 mmHg / 0.1 ~ 0.21 kg / ㎠ Heating, followed by heating at 100 ± 1 ° C. for 180 ± 5 minutes, followed by heating at 95 ± 1 ° C. for 60 ± 3 minutes, is suitably concentrated two to five times in succession, but more preferably Concentrate four times in succession.

The steaming and cooling step, after steaming the concentrated extract for 36 to 48 hours and cooled under a certain temperature, includes the step of storing under 1.5 ~ 3.5 ° C.

Through the steps (steps) described above, arrowwood containing sodium succinate to lower the blood sugar level of the human body and activate insulin secretion, oak mistletoe to increase the anti-cancer effect and immunity, and lower the blood cholesterol level and rich in various amino acids And there is an advantage to be able to produce a functional arrow juice having all the pharmacological effects of shiitake mushrooms to increase the body's immunity.

Hereinafter, to describe the 'arrow arrow healthy rice' manufacturing method through the arrow juice prepared by the above-described process.

First, the arrowhead juice is administered to the washed rice in the rice cooker instead of water. The dosage is approximately 200-330 ml in 2-3 packs compared to 90-100 g of white rice (white rice). If the arrow juice is administered less than the dose is less than the expected synergistic effect according to the arrow juice, when administered a lot of the less cost-effective synergistic effect may cause economic problems. However, since the dosage is variable according to the preference of the intake, it should be noted that the scope of the present invention is not limited thereto.

After the rice is finished, open the pot lid and mix. The arrow juice is a relatively thick sediment is settled in the lower part of the cooker should be subjected to the step of mixing it.

Hereinafter will be described in detail the arrow juice contained in the arrow gobap according to an embodiment of the present invention. However, it should be noted that the scope of the present invention is not limited by the following examples.

Arrow juice  Produce

The ingredients were added to the concentrator according to each composition ratio of the examples in Table 1, followed by heating at 120 ° C. for 120 minutes at an input vacuum of 645 mmHg / 0.15 kg / cm 2 and then heating at 100 ° C. for 180 minutes, followed by 60 at 95 ° C. Heating for one minute was concentrated four times in succession in one cycle and then steamed for 40 hours. And cooled with 7 ℃ cooling water and then filtered to prepare an arrow juice.

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 General Shiitake Mushroom Extract - - - - 40 Arrow Mushroom Extract 30 35 40 50 - Advanced dilution 10 13 18 20 18 Licorice Extract 0.08 0.1 0.15 0.2 0.15 Purified water Quantity Quantity Quantity Quantity Quantity

Experimental Example  Antioxidant Activity of Arrow Juice

The antioxidant activity of arrow juice containing arrow mushroom extract was measured by electron donating ability or radical scavenging ability, reducing power and nitrite scavenging ability.

Electron donating ability is determined by the method of Blois (Blios MS (1958) Antioxidant determination by the use of a stable free radical.Nature 26: 1199-1200) in 0.4 mL DPPH (1,1diphenyl-) in 0.2 mL of the solution. 0.8 mL of 2-picryl-hydrazyl) solution was added to the solution, left on the vortex for 10 minutes, and the absorbance was measured at 525 nm. The formula was calculated as electron donating ability (%) = 100-[(absorbance of sample / absorbance of control) x 100]. The activity was calculated by

Reducing power was measured in 1 mL of solution according to the method of Saeedeh & Asna (Saeedeh AD, Asna U (2007) Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves.Food Chem 102: 1233-1240). 2.5 mL of 0.2 M phosphate buffer (pH 6.6) and 2.5 mL of 1% potassium ferricyanide solution were added, followed by reaction for 30 minutes at 50 ° C. Then 10% trichloroacetic acid (TCA) ) 2.5 mL of solution was added and centrifuged at 1,650 xg for 10 minutes. 2.5 mL of supernatant was added 2.5 mL of distilled water and 0.5 mL of 0.1% FeCl3 solution, and the absorbance was measured at 700 nm.

Nitrite scavenging activity was determined by Kato et al. (Kato H, Lee IE, Chuyen NV, Kim SB, Hayase F (1987) Inhibition of nitrosamine formation by nondialyzable melanoidins.Agri. Bio. Chem. 51: 1333-1338) 1 mL of the solution was added to 1 mL of 1 mM NaNO solution, and 0.1 N HCl and 0.2 M citrate buffer (pH 2.5) were added to adjust the total volume to 10 mL. After 1 hour of reaction at 37 ° C, 1 mL was added, and 0.4 mL of Griess reagent (1% sulfanilic acid: 1% naphthylamine = 1: 1) dissolved in 3 mL of 2% acetic acid solution and 30% acetic acid solution was added sequentially. After standing at room temperature for 15 minutes, the absorbance was measured at 520 nm. Distilled water was used instead of Griess reagent and the control was calculated by the formula, nitrite scavenging ability (%) = 100-[(absorbance of the sample / absorbance of the control) x 100].

Experimental results according to the experimental example of the present invention was calculated as the mean ± standard deviation (Mean ± SD) using a statistical program (SPSS ver. 12.0, SPSS Inc., Chicago, IL, USA). Statistical analysis according to statistical significance test between each group was carried out by Ducan's multiple range test when ANOVA (one-way analysis of variance test) was significant and p value was less than 0.05.

The antioxidant activity of arrow juice was shown as the following table, and Examples 2 to 4 contained the arrow shiitake mushroom extract in the range of 35 to 50% by weight compared to the functional beverage containing the general shiitake mushroom extract of Comparative Example 1. Activity was shown (p <0.05). Example 4 containing more than 40% by weight arrow shiitake extract, 18% by weight of dilute water and 0.15% by weight licorice did not show a significant difference from Example 3 (p> 0.05). In addition, Example 1 containing less than 35% by weight arrow shiitake extract, 13% by weight of dilute water and 0.1% by weight licorice showed a significant difference from Examples 2 to 4 (p <0.05).

Antioxidant activity Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Electron donating ability (%) 37.54 ± 5.87 45.48 ± 2.34 * 58.50 ± 3.50 * 58.67 ± 4.56 * 32.54 ± 4.70 Reducing Power (OD700) 0.27 ± 0.08 0.36 ± 0.07 * 0.44 ± 0.04 * 0.43 ± 0.05 * 0.24 ± 0.09 Nitrite scavenging ability (%) 12.41 ± 1.14 18.63 ± 0.93 * 25.56 ± 1.43 * 27.47 ± 2.37 * 10.81 ± 0.63

-Test results are expressed as mean ± standard deviation.

* Statistically significant difference from Comparative Example 1 (p <0.05)

Experimental Example  2. Arrow juice  Immune activity

The immunological activity of arrow juice containing arrow shiitake extract was determined by MTT assay (Sugawara I Kimoto M, Fujimot M, Ishizaka S, Tsuji T, Nishiyama T (1984) MTT assay, rapid colorimetric assay applicable to cellular proliferation and cytotoxicity.Igakuno Ayuni 123: 733-735), mitogen activity was measured. That is, spleens were aseptically extracted from 6-week-old mice (BALB / c), and the cells were recovered and adjusted to 2 × 10 ⁵ / mL. 100 μL was then dispensed into each well of a 24-well plate. Next, 100 μL of each sample was added to each concentration, and cultured for 72 hours, and then the relative activity was compared.

The immune activity of arrow juice containing arrow shiitake extract was shown in FIG. 3. When the shiitake mushroom and arrow mushroom extract were added, the immunological activity was increased compared to the control without the sample. However, the degree of increase in immune activity is greater than that of Comparative Example 1 containing a normal shiitake mushroom extract in Examples 2 to 4 containing more than 35% by weight of arrow shiitake mushroom extract, 13 wt% of advanced dilution water and 0.1 wt% licorice. Significantly high immune activity was shown (p <0.05). Example 4 containing more than 40% by weight of the arrow shiitake extract, 18% by weight dilution water and 0.15% by weight licorice showed a similar immunological activity as in Example 3 (p> 0.05)

Experimental Example  3. Arrow juice  Cancer cell growth inhibitory effect

To investigate the anticancer effect of arrow juice containing arrow mushroom extract, various cancer cell lines (human lung cancer cell, A-549 cell; human cervical cancer cell, HeLa cell; human liver cancer cell, HepG2 cell, human colon cancer cell, HT- 29 cells) were evaluated for anticancer activity. Cultivation was performed using RPMI 1640 medium or DMEM medium containing 10% FBS, 100 units / mL of penicillin and 10 μg / mL of streptomycin. . When cancer cells proliferated to 4 x 10 ⁵ / mL, washed with PBS, separated with trypsin-EDTA, and dispensed 1 x 10 ⁶ cells in culture plate (NUNC, 35 mm) in 37 ℃, 5% CO2 incubator After culturing for 24 hours, it was transferred to fresh medium containing arrow juice and incubated again for 24 hours. The inhibition rate of cancer cell proliferation was measured by 0.4% Trypan blue assay.

As a result of evaluating the cancer cell growth inhibitory activity of arrow juice containing arrow shiitake extract, it was found that human lung cancer cells and A-549 cells were found in Examples 3≥Example 4> Example 2''Comparative Example 1 and Example 1. Showed inhibitory activity. In the case of human cervical cancer cells and HeLa cells, the inhibitory activity was shown in the order of Example 4≥Example 3> Example 2> Comparative Examples 1 and 1, and Example 3≥ was performed on human liver cancer cells and HepG2 cells. Example 4> Example 2 >> Example 1, the comparative example 1 was shown inhibitory activity. Human colon cancer cells and HT-29 cells showed inhibitory activity in the order of Example 4≥Example 3> Example 2> Comparative Example 1 and Example 1.

Cancer cell line
Cancer cell growth inhibitory activity (%) Example 1 Example 2 Example 3 Example 4 Comparative Example 1 A-459 9.3 ± 4.7 15.1 ± 2.5 16.1 ± 2.5 15.1 ± 2.5 10.1 ± 2.5 HeLa 11.7 ± 3.5 13.5 ± 4.2 14.5 ± 3.2 15.5 ± 4.2 12.5 ± 4.2 HepG2 12.4 ± 4.1 14.1 ± 3.4 17.2 ± 4.3 16.4 ± 2.4 12.1 ± 3.4 HT-29 13.1 ± 3.7 16.7 ± 2.4 17.4 ± 4.7 18.2 ± 4.1 13.7 ± 4.1

Experimental Example  4. Arrow juice  Increase blood sugar and exercise performance

In order to compare the glycemic-enhancing effect and exercise performance of the arrow mushroom extract of the present invention, arrow mushroom extract was added to the negative water and fed to Sprague-dawley (SD) rats for 4 weeks. The SD rats were individually bred in a stainless steel cage in a kennel with a temperature of 22 ° C., a humidity of 55% and a contrast every 12 hours. Feed was fed pellet feed (Samyang, Korea) during the experiment.

The experimental group was set to the general drinking group (control), arrow mushroom extract administration group and arrow mushroom (bubble washing arrow mushroom) extract administration group bubble-washed dried and cut arrow shiitake mushrooms with dilution of arrow tree extract.

Arrow mushroom extract and bubble wash Arrow mushroom extract was prepared to include 40% by weight arrow extract, 18% high dilution water, 0.15% licorice extract and a suitable amount of purified water.

To determine the effect of arrow mushroom extract on glycemic control, arrow extracts were negatively administered to SD rats for 4 weeks, and blood was collected from the tail vein, and blood glucose was collected using a simple blood glucose meter (ACCU-CHEK® Roche, Switzerland). Measured.

In addition, the arrow extract was negatively administered to SD rats for 4 weeks in order to evaluate exercise performance improvement, and then forced swimming time was measured. After 12 hours of forced swimming, only negative water was fasted and fasted, and then a weight of 10% of the weight was applied to the upper end of the tail of the SD rat in a 150 cm diameter X 80 cm tank. The swimming holding time was recorded by measuring the time from the start of swimming until the floating posture of not swimming is 10 seconds or more. In addition, the judgment of exhaustion was judged that the rat did not float on the surface for 7 seconds in the water.

Referring to Figure 4, the test group administered the arrow mushroom extract reduced blood glucose compared to the control group (P <0.05). The arrowhead juice containing arrowhead mushroom extract, which had been bubble-washed from dried and cut arrow shiitake mushrooms with dilution water, decreased to 115 mg / dL, the normal blood glucose level of SD rats. Therefore, the arrow juice containing arrow mushroom extract has the effect of reducing blood sugar to normalize, it was found that the effect is further increased when bubble washing with arrow extract dilution water.

Referring to FIG. 5, the feeding time of arrow juice was increased for 4 weeks, and the swimming retention time of SD rats was significantly increased in the group administered arrow juice containing arrow mushroom extract (p <0.05). Therefore, arrow juice containing arrow mushroom extract has the effect of increasing the exercise performance through the activation of metabolism, and it was found that the effect is further increased by bubble washing with arrow extract dilution water.

The foregoing detailed description illustrates the present invention. In addition, the above-mentioned content shows and describes preferred embodiment of this invention, and this invention can be used in various other combinations, changes, and environments. That is, changes or modifications may be made within the scope of the concept of the invention disclosed in the present specification, the scope equivalent to the disclosed contents, and / or the scope of the art or knowledge in the art. The above-described embodiments illustrate the best state for implementing the technical idea of the present invention, and various modifications required in the specific application field and use of the present invention are possible. Thus, the detailed description of the invention is not intended to limit the invention to the disclosed embodiments.

Claims (7)

Providing a distilled water of arrowwood extract using a needle bar for culture on oak sawdust medium;
Drying and cutting the cultured shiitake mushrooms (arrow shiitake mushrooms) by absorbing the dilution water of the arrow-tree extract;
Bubble washing the dried and cut arrow shiitake mushrooms with distilled water of arrowwood extract;
Mixing and concentrating purified water and licorice together with an arrow shiitake in a heat concentrator;
Extracting and filtering the arrow shiitake extract;
Mixing and steaming the extract dilution water of arrowwood and oak mistletoe to the arrow shiitake mushroom extract;
It comprises; the arrow juice prepared by the above step is administered to the white rice washed in the rice cooker;
The arrowwood extract dilution water provided by using the needle bar for the medium on the oak sawdust medium is made by mixing 100 ~ 200g arrowwood extract in dilution water 20ℓ,
The arrow juice is arrow shiitake mushroom extract 35 ~ 50% by weight, dilution of 13 ~ 20% by weight of the arrowwood and oak mistletoe, licorice 0.1 ~ 0.2% by weight, and the method of producing arrowhead health rice, characterized in that consisting of purified water . The method of claim 1,
The amount of arrow juice is administered to the white rice arrow arrow healthy rice production method, characterized in that 200 ~ 330 ㎖ compared to 90 ~ 100g.
delete The method of claim 1,
The arrowwood extract dilution water provided to the oak sawdust medium through the step of providing the arrowwood extract dilution water is
Drying the leaves and branches of the arrow tree washed after being harvested; Cutting the dried leaves and branches into a predetermined size; Mixing the cut leaves and branches with an amount of water and concentrating with a heat concentrator; Cooling the arrow extract extracted through the concentration step; The method of producing an arrow and healthy rice comprising the; step of storing the cooled arrowwood extract under 1.5 ° C ~ 3.5 ° C.
delete The method of claim 4, wherein
The arrowwood extract dilution water provided to the oak sawdust medium through the step of providing the arrowwood extract dilution water is
Arrow gogi healthy rice manufacturing method characterized in that the spraying once averaging 7 times on the medium periodically even after being supplied to the medium needle.
The method of claim 1,
The drying and cutting step of the arrow mushroom
The arrow shiitake mushroom is dried for 2 to 4 hours at 25 ° C. After the arrow shiitake mushrooms cut 3 to 4 equal parts, arrow gogi healthy rice manufacturing method characterized in that the low-temperature aging under 1.5 ~ 3.5 ° C.

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