KR100747502B1 - An anti-irritation complex for skin, and a cosmetic composition containing the same - Google Patents

Abstract

A skin irritation-relieving complex and a cosmetic composition containing the same complex are provided to improve skin irritation-relieving synergistic effect by stabilizing the skin-irritating materials with nanoliposome, and maximize effects of effective ingredients including skin barrier strengthening, skin moisturizing, anti-inflammation in the skin, and suppression of erythema in the skin by UV. The skin irritation-relieving complex is a nanoliposome containing 0.1-20.0 wt.% of effective ingredients including ceramide, mung bean(Phaseolus aureus) extract, Betula platyphylla Japonica extract and Rumex crispus extract, wherein the nanoliposome is made with a high pressure homogenizer and has an average particle diameter of 10-500 nm. The cosmetic composition for relieving the skin irritation comprises 0.01-20.0 wt.% of the skin irritation-relieving complex.

Description

An anti-irritation complex for skin, and a cosmetic composition containing the same}

The present invention relates to a skin irritation complex and a cosmetic composition containing the same, and more specifically, ceramides, mung bean extracts, birch extracts, and sprout extracts showing different stimulation relaxation mechanisms are stabilized with nanoliposomes, thereby synergistic relaxation. It relates to a skin irritation complex showing a (Synergistic Effect) and a cosmetic composition containing the same as an active ingredient.

In general, many skin-related products contain substances that can cause irritation, inflammation, and allergies, so over 10% of people who use personal care products complain of side effects on the skin. In the 1990s, a survey of consumers in the United States found that more than 50% thought they were sensitive skin. In addition, recent cosmetics are focused on the functional cosmetics (cosmeceutical) that can give a real effect (skin), the skin side effects of the use of personal care products such as cosmetics is expected to increase gradually.

Increasing proportion of people who feel sensitive skin is increasing the amount of ultraviolet radiation due to the destruction of the ozone layer, drying of the living space, constitution of allergies, stress, and the increase of chemical harmful substances. The shift to nature-friendly, hypoallergenic is also believed to be a factor in improving awareness of sensitive skin. Therefore, there is a growing interest in substances that alleviate the irritation of cosmetics.

Various ingredients are used to make cosmetics, and usually 20 to 50 types of ingredients are used. These ingredients have been proved to be safe through skin irritation tests, but they can be applied to the product to cause irritation such as inflammation, itching and allergies on the skin. These reactions do not happen to everyone, and may only happen to certain people.

Conventionally, various methods have been tried to alleviate skin irritation, and for example, a method of removing a substance causing skin irritation has been attempted. However, this research is limited because the functional raw material itself often acts as a stimulus source. For example, lactic acid, glycolic acid, salicylcic acid, retinoids, etc. are contained in cosmetics to promote skin cell regeneration or anti-wrinkle effects but cause skin irritation. do. Accordingly, in recent years, many studies have been conducted to relieve stimulation by neutralizing acidity by adding strong alkalis such as NaOH or KOH to stimulus-inducing functional raw materials, but such a prescription has a problem of lowering the efficacy of existing raw materials.

Accordingly, a main object of the present invention is to provide a complex of stimulating complex stabilized with nanoliposomes having excellent skin stimulating effect.

Another object of the present invention to provide a cosmetic composition containing the irritant complex.

In order to achieve the above object, the present inventors conducted various studies and numerous tests to find a substance having a stimulating alleviation effect while maintaining the effect of a functional raw material as it is, and as a result, excellent stimulating alleviation effects with different mechanisms. It was found that the irritation-releasing complexes stabilized with visible ceramides, mung bean extracts, birch extracts, and sperm extracts with nanoliposomes showed a synergistic relaxation effect. It was confirmed that the present invention can be alleviated and the present invention has been completed.

In order to achieve the above object, the present invention provides a stimulation-releasing complex stabilized with nano liposomes ceramide, mung bean extracts, birch extracts and rubbing extracts as an active ingredient having a different stimulation relaxation mechanism.

In addition, there is provided a cosmetic composition containing the stimulating relaxation complex stabilized with the nanoliposomes.

Hereinafter, the present invention will be described in more detail.

Ceramide used as an active ingredient of the present invention is an intercellular lipid component, the intercellular lipid is a main component of the stratum corneum which constitutes the outermost layer of the skin. The structure of the stratum corneum consists of protein-rich keratinocytes and intercellular lipids filling them.In the mid-1970s, Elias et al. Formed a lamellar structure between cells in which the lipid components in the stratum corneum were keratinized. It acts like cement and has been reported to be the source of skin barrier function (Arch. Dermatol. Res. 27: 95-117 (1981)).

The structure of the stratum corneum is described as a two-compartment model, which is called the brick and mortar model in comparison to the mortar, which is the brick when the walls are stacked and the adhesive that is filled between them. Int. J. Dermatol. 20: 1-19 (1981). When the barrier function is weakened due to abnormalities of the stratum corneum of the skin, it is understood that various skin diseases occur or worsen.

Since the masonry and mortar models have been published, many studies have been conducted on the lipid components of keratinocytes, suggesting that the ceramides, cholesterol and free fatty acids that make up the stratum corneum lipids are important for maintaining the barrier function and lamellar structure of the stratum corneum. It is recognized.

These intercellular lipids are in principle composed of about 50% ceramide, about 20-25% cholesterol, about 20-25% free fatty acid, about 10% cholesterol ester and about 1-2% cholesterol sulfate. However, little or no phospholipids are present.

The major difference between the lipids of the stratum corneum and other tissues of the body is that the major constituent lipids are ceramides, cholesterol, fatty acids and the like, and the carbons of the lipids constituting ceramides have a relatively long chain carbon distribution of 16 to 33. In addition, the carbon number of free fatty acids is also very high in C ₂₂ and C ₂₄ . Thus, the carbon distribution is much longer than that of the phospholipids present in the biological lipid membrane.

As such, it is not easy to penetrate these epidermal lipid species into the skin by the very long carbon distribution of the intercellular lipid species, but using nanoliposomes according to the present invention increases the skin penetration effect of these intercellular lipid components, thereby improving the stimulating effect. It can be maximized. Meanwhile, studies using liposomes have been actively conducted by various researchers. However, most of its uses promote the percutaneous absorption of active ingredients (Korean Patent Application Nos. 2000-0008212 and 2000-0042399), Enhance skin moisturization (Korean Patent Application No. 1994-7004646), and improve atopic dermatitis and dry skin. (Korean Patent Application No. 2000-0027165) and the like.

Mung bean (綠 豆) used as another active ingredient of the present invention is a perennial herb belonging to the dicotyledon rose rosaceae, its origin is estimated to be India, and is mainly distributed in Asian regions such as Korea, China and India. Mung beans grow well on warm climate loam (a black soil mixed with sand and clay) and are about 30-80 cm in height. The stem has a thin, vertical vein, about 10 nodes, and branches. The leaves come out with a pair of cotyledons and freshly formed leaves, followed by three leaflets. Flowers are yellow and bloom in August, gathering several on axilla, but only 3-4 pairs bear fruit. Fruits are edible, initially green, but ripened and covered with long, coarse fur. It is 5-6cm long and contains 10-15 seeds in one pod. Seeds are often green, but yellow, greenish brown, and blackish brown. The varieties are divided into yellow, greenish brown, and blackish brown mung beans, depending on the color of the seeds, but green mung beans make up 90% of the total. The ingredients are 53-54% starch and 25-26% protein.

Mung beans (Phaseolus aureus, Phaseolus radiatus, Mung Bean, Green Bean) are originally widely used as antipyretics and antidote in oriental medicine because they have strong antipyretic and detoxifying effects. It is used for jade yongsushi acid, which makes your face look like jade, yeooksan, which is effective for removing skin impurities and dry skin, and yeogiyeooksan, which removes blemishes, and Oknaeyoung, which removes sweat bands that occur in summer. Mung beans have also been used effectively in yellow spear (bush), carbuncle (boil) and mumps (pandemic mumps), which are caused by the penetration of dermatophytes into the stratum corneum of the skin. According to the inventors, the mung bean extract has an excellent effect of increasing the resistance to the stimulating agent of skin cells through antioxidant activity, free radical scavenging action, skin cell activity and regeneration, and shows histamine release effect. Indicates.

The birch tree (Betula Platyphylla Japonica), which is used as another active ingredient of the present invention, is a tree belonging to the birch family, which grows in the mountainous region of the northern part, reaching 20m in height. Bark is white, peeled horizontally, and young branches are reddish brown with spots. Leaves are triangular ovate, 5-7 long, pointed at the end, sawtooth at the edge, petioles about 2 long. Flowers bloom in April and May, and female flowers bloom in the same season and at the same tree with long doe flowers. The fruit ripens in September, with wide wings from side to side. Fruit-bearing cylindrical sacks down 4 long. The juice of the tree is used for nourishing tonic and skin diseases, but very weak for urban pollution. Birch bark has been used as an important medicine as a medicine, it acts on the liver, lowers heat, removes moisture, stops coughing and cuts off the walls. In addition, the detoxification effect is very strong to remove inflammation, and diuretic effect can be used to relieve nephritis or edema, oriental medicine and folk medicine called baekhwapi, hides and jaundice, diarrhea, nephritis, pulmonary tuberculosis, gastritis, various carbuncles It has been used to treat the back.

As confirmed by the present inventors, the birch extract has a characteristic of preventing the destruction of keratinocytes and fibroblasts caused by the ultraviolet rays in the human body, thereby inhibiting radical activation by ultraviolet rays. In addition, it is possible to prevent the harmful action by ultraviolet irradiation as well as to show the effect of inhibiting the production of inflammatory mediators such as arachidonic acid, prostaglandin, leukotriene and the like, showing an excellent stimulating effect.

Rumex crispus (Rumex crispus) is another active ingredient of the present invention is called yangjegeun, solbang, muzzle, etc., is a perennial grass of the perennial herb that dries up in the summer and grows again when cool. Young leaves are eaten as herbs, and are said to be effective for ringworm, athlete's foot, and eczema. Since ancient times, it has been used for skin diseases such as amelioration, scabies and skin cancer, and it is also used for cystitis and colitis. It is called Yangje-geun when the roots are cracked and rolled up in the autumn and dried in the sun.

According to the present inventors, the sperm extract has a very good effect of suppressing the secretion and action of elastase and hyaluronidase, and excellent anti-inflammatory effect through the inhibition of inflammation mediated cytokines such as IL-8 (Interleukin-8). Inflammatory effect.

Mung bean extract, birch extract, and rume extract used in the present invention can be obtained using various extraction methods known in the art.

Preferably, (a) water, (b) anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms (methanol, ethanol, propanol, butanol, etc.), (c) a mixed solvent of the lower alcohol with water, (d) acetone , (e) ethyl acetate, (f) chloroform or (g) 1,3-butylene glycol can be obtained as an extraction solvent.

On the other hand, it is apparent to those skilled in the art that such herbal extracts can be obtained in addition to the above-described extraction solvents, and extracts having substantially the same effect using other extraction solvents.

In the present invention, the extract includes not only the extract by the above-described extraction solvent, but also an extract that has undergone a conventional purification process. Obtained by various additional purification methods, such as, for example, separation using ultrafiltration membranes having a constant molecular weight cut-off value, separation by various chromatography (manufactured for separation according to size, charge, hydrophobicity or affinity). The fraction is also included in the extract of the present invention.

In addition, the extract of the present invention may be prepared and used in powder form by an additional process such as distillation under reduced pressure and freeze drying or spray drying.

According to the present invention, when the ceramides, mung bean extracts, birch extracts, and sperm extracts showing different stimulation relaxation mechanisms are stabilized with nanoliposomes, a stimulation-releasing complex having a synergistic effect is obtained.

As used herein, the term nanoliposomes refers to liposomes having an average particle diameter of 10-500 nm as having the form of conventional liposomes. According to a preferred embodiment of the invention, the average particle diameter of the nanoliposomes is 100-300 nm. When the average particle diameter of the nanoliposomes exceeds 300 nm, the improvement of skin penetration and the improvement of formulation stability are very weak among the technical effects to be achieved in the present invention.

According to the present invention, the nanoliposomes used to stabilize ceramides, mung bean extracts, birch extracts, and borage extracts are prepared by a mixture comprising polyols, oily ingredients, surfactants, phospholipids, fatty acids and water, and monohydric alcohols ( Eg ethanol).

The polyol used in the nanoliposomes of the present invention is not particularly limited, preferably propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methylpropanediol, isopropylene glycol, Pentylene glycol, erythritol, xylitol, sorbitol and mixtures thereof. The amount used is 10-80% by weight, preferably 30-70% by weight based on the total weight of the nanoliposomes.

The oil component used in the preparation of the nanoliposome of the present invention may be a variety of oils known in the art, preferably a synthetic oil dimethicone of hydrocarbon-based oil esters such as hexadecane and paraffin oil. And animal and vegetable oils such as silicone oils such as cyclomethicone, sunflower oil, corn oil, soybean oil, avocado oil, sesame oil and fish oil ethoxylated alkyl ether oil propoxylated alkyl ether oil phytosphingosine, Sphingoidoid lipids such as sphingosine and sphinginine Cerbroside Cholesterol Cholesterol Cholesterylsulfate Citosterylsulfate C10-40 fatty alcohols and mixtures thereof. The amount used is 1.0-30.0% by weight, preferably 3.0-20.0% by weight based on the total weight of nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art. For example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants can be used. Preferred are anionic surfactants and nonionic surfactants. Specific examples of anionic surfactants include alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkylethers, alkoxylated alkylesters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Particularly preferred surfactants are polysorbates belonging to nonionic surfactants. The amount of the surfactant to be used is generally 0.1-10% by weight, preferably 0.5-5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. In particular, naturally occurring unsaturated or saturated lecithins extracted from soybean or egg yolk are preferred. Typically, lecithin derived from nature has an amount of phosphatidylcholine of 23-95%, and an amount of phosphadidylethanolamine of 20% or less. In preparing the nanoliposomes of the present invention, the amount of lecithin used is 0.5-20.0% by weight, and preferably 2.0-8.0% by weight, based on the total weight of the nanoliposomes.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of the C12-22 alkyl chain, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. Include. The amount used is 0.05-3.0 wt% with respect to the total weight of nanoliposomes, preferably 0.1-1.0 wt%.

Water used for the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is used is 5.0-40% by weight relative to the total weight of nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by high pressure homogenizer can be carried out under various conditions (eg pressure, frequency, etc.) depending on the desired particle size, preferably 1-5 times high pressure homogenase under pressure of 600-1200 bar. Nanoliposomes are prepared by passing through the Azure.

Preferably, the total content of the ceramide, mung bean extract, birch extract, and rume extract, which are the active ingredients contained in the stimulation-releasing complex of the present invention, is 0.1-20.0% by weight, more preferably 1.0-10.0% by weight, based on the weight of the nanoliposome. %to be.

Ceramides contained in the stimulation-releasing complex of the present invention are very effective in enhancing skin barrier function, preventing skin drying through inhibiting moisture and electrolyte loss, and inhibiting skin penetration of irritants, and mung bean extracts have antioxidant activity and free radical scavenging. It has the effect of increasing the skin cell's resistance to the stimulating source through the action, skin cell activity and regeneration, and histamine release effect, and the birch extract is a keratinocyte and fibroblast inside the human body caused by ultraviolet rays, etc. It has the characteristic of preventing the destruction of (fibroblast), and can inhibit radical activation by ultraviolet rays, prevent harmful effects caused by ultraviolet irradiation, and also prevent the inflammatory mediators such as arachidonic acid, prostaglandin, leukotriene, etc. Show excellent anti-irritant effect Soruja extract is very effective in suppressing the secretion and action of elastase and hyaluronidase, and has excellent anti-inflammatory effect through inhibition of secretion of inflammatory mediated cytokines such as IL-8 (Interleukin-8). Indicates.

In addition, the cosmetic composition according to another aspect of the present invention contains the above-mentioned irritation-releasing complex stabilized by nanoliposome stabilization of ceramide, mung bean extract, birch extract, and sperm extract as an active ingredient, and has excellent stability and skin penetration This excellent effect of the active ingredient is maximized, showing an excellent effect on skin protection.

Preferably, in the cosmetic composition of the present invention, the content of the irritation-releasing complex, ie, nanoliposomes, is 0.01-20.0% by weight, more preferably 0.1-10.0% by weight, based on the total weight of the cosmetic composition.

Ingredients included in the cosmetic composition of the present invention contains components commonly used in cosmetic compositions in addition to the irritation-complexing complex stabilized with ceramides, mung beans extracts, birch extracts and russ extracts as nanoliposomes as an active ingredient, such as stabilizers , Conventional adjuvants such as solubilizers, vitamins, pigments and flavorings, and carriers.

Cosmetic compositions of the present invention may be prepared in any formulation conventionally prepared in the art, for example, solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, and the like. It may be formulated as, but is not limited thereto. More specifically, it may be prepared in the form of a flexible lotion, nutrition lotion, nutrition cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack.

When the formulation of the present invention is a paste, cream or gel, carrier oils include animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silica, talc or zinc oxide. Can be used.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , Fatty acid esters of 1,3-butylglycol, oils, glycerol aliphatic esters, polyethylene glycols or sorbitan.

When the formulation of the present invention is a suspension, the carrier component is water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester. , Microcrystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing cosmetic, as a carrier component, an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The stimulation-releasing complex according to the present invention is a ceramide, mung bean extract, birch extract, Soruja extract showing different stimulation relaxation mechanism is stabilized with nano-liposomes showing a synergistic relaxation effect. The anti-irritant complex of the present invention has excellent effects such as enhancing skin barrier function, preventing skin drying through inhibiting moisture and electrolyte loss, inhibiting skin penetration of irritants, and enhancing antioxidant, free radical scavenging, skin cell activity and regeneration. Increasing resistance to irritants of skin cells through the skin, inhibiting histamine release, preventing harmful action by ultraviolet light, inhibiting the production of inflammatory mediators such as arachidonic acid, prostaglandin, leukotriene, etc., inhibiting the secretion and action of elastase, hyaluronidase Inhibition of inflammation-mediated cytokine secretion results in an excellent stimulating effect.

Moreover, the irritation-releasing complex of the present invention by nanoliposomalizing the active ingredient ceramide, mung bean extract, birch extract, sloe extract, the cosmetic composition containing it has excellent stability, excellent skin penetration power of the active ingredient The effect is maximized to provide excellent skin protection.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it is to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. Will be self-evident. In addition, the part,%, and ratio used in this Example are a mass reference | standard unless there is particular notice.

(Manufacture example 1)

Preparation of Mung Bean Extract

10 kg of 70% ethanol aqueous solution was added to 1 kg of mung beans, and the mixture was left to stand at room temperature for 4 days, followed by filtration. The filtrate was concentrated under reduced pressure, and dried at 40 ° C. for 24 hours to obtain 22.5 g of a light green powder. 980 g of purified water: butylene glycol (1: 1 weight ratio mixture) was added to 20 g of this powder, which was then stirred at room temperature to completely dissolve to obtain 1 kg of mung bean extract.

(Manufacture example 2)
Preparation of Birch Extract

10 kg of 70% ethanol aqueous solution was added to 1 kg of birch bark and left to stand at room temperature for 4 days. After filtering, the filtrate was concentrated under reduced pressure and dried at 40 ° C for 24 hours to obtain 85 g of a brown powder. 980 g of purified water: butylene glycol (1: 1 weight ratio mixture) was added to 20 g of this powder, which was then stirred at room temperature to completely dissolve to obtain 1 kg of birch extract.

(Manufacture example 3)

Preparation of Soruja Extract

5 kg of 70% ethanol aqueous solution was added to 1 kg of the root of Soru-Jin, and the mixture was left to stand at room temperature for 4 days. After filtering, the filtrate was concentrated under reduced pressure and dried at 40 ° C. for 24 hours to obtain 105 g of a light green powder. 980 g of purified water: butylene glycol (1: 1 weight ratio mixture) was added to 20 g of this powder, which was then stirred at room temperature to completely dissolve to obtain 1 kg of an extract.

(Manufacture example 4)

Preparation of Nanoliposomes

The stimulation-releasing complex stabilized with nanoliposomes was added to phase B with A in the composition of Table 1 below, uniformly wet, and then phase C was added and heated and mixed to 70-80 ° C. The solution was passed through a high-pressure homogenizer 2-4 times at 600-1000 bar and cooled to obtain a 100-300 nm diameter particle size complex.

Further, in addition to the irritation-releasing complex (Example) containing all 5% by weight of ceramide, mung bean extract, birch extract, and rust extract each, Comparative Example 1 containing only 20% by weight of ceramide, 20% by weight of mung bean extract Comparative Example 2, Comparative Example 3 containing only 20% by weight of the birch extract, Comparative Example 4 containing only 20% by weight of Soru-ja extract was prepared, as a control example the ceramide, mung bean extract, birch extract, Nanoliposomes that do not contain an extract

Prize ingredient Content (% by weight) Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Control A glycerin To 100 To 100 To 100 To 100 To 100 To 100 B Hydrogenated lecithin 5.0 5.0 5.0 5.0 5.0 5.0 Floating paraffin 1.0 1.0 1.0 1.0 1.0 1.0 Macadamia Nut Oil 1.0 1.0 1.0 1.0 1.0 1.0 Squalene 1.0 1.0 1.0 1.0 1.0 1.0 Dimethicone 1.0 1.0 1.0 1.0 1.0 1.0 Isopropyl myristate 1.0 1.0 1.0 1.0 1.0 1.0 Sodium Stearoyl Lactate 0.5 0.5 0.5 0.5 0.5 0.5 Polysorbate-80 0.5 0.5 0.5 0.5 0.5 0.5 Ceramide 5.0 20.0 - - - - C Mung Bean Extract 5.0 - 20.0 - - - Birch Extract 5.0 - - 20.0 - - Noisy Extract 5.0 - - - 20.0 - Potassium hydroxide Quantity Quantity Quantity Quantity Quantity Quantity Purified water 5.0 5.0 5.0 5.0 5.0 5.0

Experimental Example 1

Recovery test after skin barrier injury in hairless mice

Acetone was applied twice a day to hairless mice 8-10 weeks after birth to impair the skin barrier function of the experimental animals and then evaluate their resilience. When epidermal moisture loss (TEWL) reached 4.0 g / m 2 / h by applying acetone to the embryos of hairless mice, the samples of Examples, Comparative Examples 1, 2, 3, 4 and Comparative Example of Table 1 above Was applied to a 5 cm 2 area. TEWL was measured with Tewameter TM 210, an evaporimeter from C-K (Courage + Khazaka, Cologne, Germany). After 6 hours of application, TEWL was measured to evaluate the extent to which TEWL was reduced to evaluate the extent to which skin barrier function was restored. Recovery rate calculation used in the evaluation was calculated as in the following equation (1). The results are shown in Table 2.

Br (Barrier Recovery) = [1- (B _{t = 6} -B _{t = 0} ) / (B _{t = d} -B _{t = 0} )] x 100

(B _{t = 6} : TEWL measured 6 hours after skin barrier injury, B _{t = 0} : TEWL measured before skin barrier injury, B _{t = d} : TEWL measured immediately after skin barrier injury)

Application Sample Skin barrier recovery rate (6 hours) (Initial TEWL) Example 56.9% Comparative Example 1 52.3% Comparative Example 2 26.5% Comparative Example 3 24.8% Comparative Example 4 25.4% Control 20.1%

Looking at the results of Table 2, in the case of stimulating relaxation complex (Example) stabilized ceramide, mung bean extract, birch extract, Soruk extract with nanoliposomes, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example It can be seen that it shows an excellent skin barrier recovery rate compared to 4 and the control example.

Experimental Example 2

Evaluation of water holding capacity and water evaporation amount in human application

In order to evaluate the moisturizing power of the Examples, Comparative Examples 1, 2, 3, 4 and the control example, the water retention capacity and the water evaporation rate were measured as follows on the skin to which each test substance was applied.

Experimental Example 2-1

Determination of Moisture Retention Capacity

In the constant temperature and humidity room of 22 ° C. and 45% relative humidity, the above Examples, Comparative Examples 1, 2, 3, 4 and Control Example were coated with a predetermined amount (0.01 g / 4 cm 2) inside the forearm of 20 test subjects, and before application, The moisture content of the skin after 1 hour of application and after 2 hours of application was measured. The measuring device used a moisture content measuring instrument (corneometer CM820, Courage + Khazaka, Cologne, Germany) to measure the moisturizing power by measuring the electric capacity of the skin according to the moisture content of the skin. The results are shown in Table 3 below.

division Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Control Skin electrical conductivity before application 50 50 50 50 50 50 Skin electrical conductivity 1 hour after application 82 77 61 60 61 58 Skin conductivity after 2 hours of application 78 71 57 55 56 53

Experimental Example 2-2

Measurement of water evaporation amount

After tape stripping on the forearm of the arm, the skin's protective film was weakened, and each test substance was applied to the test subject in the same manner as in Experiment 2-1, followed by CK (Courage + Khazaka, Cologne, Germany). Using a Tewameter TM 210, an evaporimeter, the water vapor evaporation was measured 5 times at 1 minute intervals every 12 hours to obtain an average value. The results are shown in Table 4.

division Example (%) Comparative Example 1 (%) Comparative Example 2 (%) Comparative Example 3 (%) Comparative Example 4 (%) Comparative Example (%) Moisture evaporation after 0 hours 100.0 100.0 100.0 100.0 100.0 100.0 Moisture evaporation after 12 hours 74.6 78.4 92.3 93.1 92.7 96.5 Moisture evaporation after 24 hours 55.3 63.2 85.6 86.3 86.1 91.6 Moisture evaporation after 36 hours 42.6 55.2 80.0 81.1 80.1 86.4 Moisture evaporation after 48 hours 35.5 50.8 77.4 78.5 78.3 82.6

As shown in the results of Experimental Examples 2-1 and 2-2, in the case of the irritation-releasing complex (Example) in which the ceramide, mung bean extract, birch extract, and rume extract of the present invention were stabilized with nanoliposomes, Example 1 Compared with Comparative Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example, the electrical conductivity of the skin is high, and the amount of water loss is small. Therefore, it can be seen that the stimulating relaxation complex of the present invention exhibits an excellent moisturizing effect.

Experimental Example 3

Evaluation of Anti-inflammatory Effects-Carrageenan Foot Edema

Example 1, Comparative Example 1, 2, 3, 4 and the control example was administered 12.5-100 mg / kg into the abdominal cavity of the mouse, respectively, 1 hour, 0.5 ml of 0.1% carrageenan solution was injected into the back of the experimental animal Caused inflammation. Changes in the paw volume of rats were measured immediately after carrageenan injection and 4 hours after injection. The results are shown in Table 5.

% Inhibition Rate = (1-ΔV Treatment / ΔV Control) ㅧ 100

(ΔV: change of foot volume)

Extract dose (mg) % Inhibition rate Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Control 12.5 23.5 11.5 15.7 13.3 14.3 6.3 25 43.2 19.5 30.8 29.8 30.4 9.5 50 60.9 25.6 41.3 40.6 40.3 10.1 100 75.3 30.6 49.5 50.1 49.5 10.4

Referring to Table 5, in the case of the irritation-releasing complex (Example) of the ceramide, mung bean extract, birch extract and Soruch extract of the present invention stabilized with nanoliposomes, Comparative Example 1, Comparative Example 2, Comparative Example 3, comparison It was found that there was an excellent anti-inflammatory effect compared to Example 4 and the control example.

Experimental Example 4

Inhibition effect of erythema formation upon UV irradiation

The color difference meter (Chromameter CR 300, Minolta) to investigate the effect of inhibiting erythema formation under UV irradiation for the ceramide, mung bean extract, birch extract and stimulus extract stabilized with nanoliposome (Example) of the present invention ) A value was calculated.

Ten healthy women (mean age 31 years) were irradiated with ultraviolet light at 0.88 J / ㎠, three times per day. Example, Comparative Examples 1, 2, 3, 4 and the control example were applied to each test site twice a day for 30 days, and Δ which was the difference between the a value before application and the a value over time after application. The a value was calculated according to Equation 3 below to compare the effect of inhibiting erythema production, and the results are shown in Table 6.

 Efficacy inhibitory effect (%) = (a value before application-a value after application) / a value before application x 100

division Inhibition of erythema production (%) Example 89.0 Comparative Example 1 53.2 Comparative Example 2 64.3 Comparative Example 3 71.5 Comparative Example 4 62.3 Control 24.5

As can be seen in Table 6, the ceramide, mung bean extract, birch extract and Sorrung extract of the present invention stimulated complexes stabilized with nanoliposomes (Example) are Comparative Example 1, Comparative Example 2, Comparative Example 3, comparison Compared with Example 4 and the control example, it showed an excellent effect of inhibiting erythema formation by ultraviolet light.

(Manufacture example 5)

Preparation of cosmetic composition containing irritant complex

A cosmetic composition was prepared containing the stimulation-releasing complex (Example) in which ceramides, mung bean extracts, birch extracts, and sperm extracts having different stimulation-releasing mechanisms of the present invention were stabilized with nanoliposomes. Cosmetic compositions containing Example 2, Comparative Example 3, Comparative Example 4, and Comparative Example were prepared. Then, a cosmetic composition containing a simple mixture that was not stabilized with nanoliposomes mixed with ceramide, mung bean extract, birch extract, and rume extract was prepared in the following composition.

ingredient Content (% by weight) Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 Example Stimulating Relaxation Complex 10.0 - - - - - - Comparative Example 1 Stimulation Relaxation Complex - 10.0 - - - - - Comparative Example 2 Stimulation Relaxation Complex - - 10.0 - - - - Comparative Example 3 Stimulation Relaxation Complex - - - 10.0 - - - Comparative Example 4 Stimulation Relaxation Complex - - - - 10.0 - - Comparative Stimulation Relaxation Complex - - - - - 10.0 - Simple mixture not stabilized with nanoliposomes mixed 1: 1, 1: 1 with ceramide, mung bean extract, birch extract, and rume extract - - - - - - 2.0      A Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Mikey-Crystalline 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Squalene 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 3.0 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 5.0 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 1.2 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopherol Acetate 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Michael Methic 3.0 3.0 3.0 3.0 3.0 3.0 3.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 0.05  B glycerin 4.0 4.0 4.0 4.0 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 2.0 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 0.05 0.05 0.05 0.05 Purified water To 100 To 100 To 100 To 100 To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity Quantity Quantity Quantity Quantity

(The content of the active ingredient in the nanoliposome is 20.0% by weight, and since 10.0% by weight of the nanoliposome is used, the content of the active ingredient is 2.0% by weight, and thus, ceramide, mung bean extract, birch extract, and sorghum The content of the simple mixture of cranberry extract is 2.0% by weight.)

Experimental Example 5

Human skin patch test

In the past, the skin irritation effect was confirmed in human skin patch test of 20 women in their 20s and 30s who had no hypersensitivity reaction to skin irritation and have no skin disease or skin allergy symptoms. Lactic acid 5.0%, a skin irritant, was added to Formulation Example 1 and Comparative Formulation Examples 1 to 6 of the present invention and used. First the test site was wiped with 70% ethanol and dried. 15 μg of the prepared test substance was dropped in a Fin chamber (Finn chamber, 100 × 10, EPITEST, Finland), and then sealed in the forearm inner part of the test subject. The patch was marked for 24 hours, the patch was removed, and the test site was marked with a pen. 1 hour and 24 hours after marking, the test site was observed using a magnifying glass (8MC-150, DAZOR, United States of America) to observe erythema and edema. Skin response was determined according to the regulations of the International Contact Dermaitis Research Group (ICDRG), and the mean skin response rate was calculated according to Equation 4. The results are shown in Table 8.

[Evaluation Criteria and Score of Skin Response]

Symbol score Evaluation standard - 0 No response ± 0.5 Faint or light erythema  + One Boundary but weak erythema, edema and papules  ++ 2 Pronounced erythema, papules and vesicles  +++ 3 Severe erythema and alveolar, scleroderma

Average skin reactivity = (score x responses x 100 x 1/2) / [3 (maximum score) x total subjects (n)]

Test substance 1 hour later 24 hours later % Reactivity (n = 20) ± + ++ ± + ++ Formulation Example 1 6 - - 2 - - 3.33 Comparative Formulation Example 1 9 One - 3 One - 6.67 Comparative Formulation Example 2 9 One - 4 One - 7.08 Comparative Formulation Example 3 9 2 - 4 2 - 7.92 Comparative Formulation Example 4 10 One - 3 One - 7.08 Comparative Formulation Example 5 13 3 - 5 3 - 12.50 Comparative Formulation Example 6 9 One - 4 One - 7.08

As can be seen in Table 8, Formulation Example 1 is a cosmetic composition containing a stimulation-releasing complex (Example) of the ceramide, mung bean extract, birch extract, and lychee extract of the present invention stabilized with nano liposomes Comparative Examples 1 ~ 4, control and skin irritation compared to Comparative Formulation Examples 1 to 6, which is a cosmetic composition containing a control compound and a simple mixture (a composition in which ceramide, mung bean extract, birch extract, and rume extract are 1: 1: 1: 1) It was found that the effect was excellent.

Experimental Example 6

Comparison of skin irritation relief effects in human application

Twenty women in their 20s and 30s were asked to use Formulation Example 1 and Comparative Formulation Examples 1 to 6 containing 5.0% of skin irritant for 10 days. Each morning and evening were used, and then questionnaires were marked for irritation and intensity. The test results were evaluated once a day and divided into subjective and objective stimuli. The results are shown in Table 9.

division Subjective stimulation (% relaxation) Objective stimulation (% relaxation) Average (% relaxation) Formulation Example 1 71 81 76.0 Comparative Formulation Example 1 42 52 47.0 Comparative Formulation Example 2 40 48 44.0 Comparative Formulation Example 3    38    48    43.0 Comparative Formulation Example 4    39    49    44.0 Comparative Formulation Example 5    12    20    16.0 Comparative Formulation Example 6    44    52    48.0

As can be seen in Table 9, Formulation Example 1 is a cosmetic composition containing a stimulation-releasing complex (Example) of the ceramide, mung bean extract, birch extract, Soruch extract of the present invention stabilized with nanoliposomes (Comparative Example 1) 4, subjective and objective compared to Comparative Formulation Examples 1 to 6, which is a cosmetic composition containing a control example and a simple mixture (a composition in which ceramide, mung bean extract, birch extract, and rume extract are 1: 1: 1: 1) Excellent effect in all skin irritation relief.

Experimental Example 7

Formulation Stability Test

The stability test of Formulation Example 1, Comparative Formulation Examples 1 to 6 of the present invention was carried out by the following method. To test the stability of the cosmetic formulation 1, Comparative Formulation Examples 1 to 6 in a opaque glass container kept in a constant temperature maintained at 45 ℃ constant storage for 12 weeks and also kept completely at 4 ℃ refrigerator After storing for 12 weeks in an opaque glass container inside, the discoloration, deodorization and degree of separation of the product was measured. The results are shown in Table 10 below. At this time, the degree of discoloration, odor and separation of the product was evaluated by classifying into the following six grades.

* Standards for evaluating product discoloration, odor and separation

0: No change 1: Very slight discoloration, odor, separation

2: a little discoloration, discoloration, separation 3: little discoloration, discoloration, separation

4: severely discolored, discolored, separated 5: extremely severely discolored, discolored, separated

division Product safety Formulation Example 1 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Comparative Formulation Example 5 Comparative Formulation Example 6 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ 45 ℃ 4 ℃ discoloration 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Deodorant 0 0 0 0 0 0 0 0 0 0 0 0 0 0 detach 0 0 0 0 0 0 0 0 0 0 0 0 0 0

As shown in Table 10, Formulation Example 1 and Comparative Formulation Examples 1 to 6 are all stable under all conditions without discoloration / odor or separation symptoms.

Based on the results of the experimental example described below, the present invention is to present a cosmetic composition containing a stimulating relaxation complex stabilized with nano-liposomes, ceramide, mung bean extract, birch extract, Soruch extract of the present invention. However, the composition of the present invention is not limited to the following formulation examples.

(Formulation Example 2)

Softener (Skin Lotion)

As shown in Table 11 below, the flexible makeup was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Example 3.0 1.3-butylene glycol 6.0 glycerin 4.0 EDTA-2Na 0.02 Sodium Hiruronate 2.0 Fiji-40 Hydrogenated Castor Oil 0.5 ethanol 15.0 Benzophenone-9 0.05 Incense, preservative a very small amount Purified water to 100

(Formulation Example 3)

Nourishing Cosmetic (Milk Lotion)

As shown in Table 12, nutritional longevity was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Example 5.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocophenyl Acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl Polymer 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

(Formulation Example 4)

Nutrition Cream

As shown in Table 13, nutrition cream was prepared according to a conventional method.

Compounding ingredients Content (% by weight) Example 15.0 Cetostearyl alcohol 2.5 Glyceryl Stearate 1.5 Trioctanoine 5.0 Polysorbate 60 1.2 Sorbitan stearate 0.5 Squalane 5.0 Floating paraffin 3.0 Cyclomethicone 3.0 BH 0.05 Delta-tocopherol 0.2 Concentrated glycerin 4.0 1,3-butylene glycol 2.0 Santa sword 0.2 EDTA-2Na 0.05 Incense, preservative a very small amount Purified water to 100

Formulation Example 5

Massage cream

Massage cream was prepared according to a conventional method as shown in Table 14.

Compounding ingredients Content (% by weight) Example 5.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 0.5 Beeswax 2.0 Tocopheryl Acetate 0.1 Polysorbate 60 3.0 Sorbitan sesquioleate 2.5 Cetearyl Alcohol 2.0 Liquid paraffin 30.0 Carboxy Vinyl Polymer 0.5 Incense, preservative a very small amount Purified water to 100

Formulation Example 6

pack

Packs were prepared according to conventional methods as shown in Table 15 below.

Compounding ingredients Content (% by weight) Example 2.0 Propylene glycol 2.0 glycerin 4.0 Carboxyvinyl Polymer 0.3 ethanol 7.0 Fiji-40 Hydrogenated Castor Oil 0.8 Triethanolamine 0.3 Bietchiti 0.01 EDTA-2Na 0.01 Incense, preservative a very small amount Purified water to 100

As described in detail above, the irritation-releasing complexes stabilized with nanoliposomes of ceramides, mung bean extracts, birch extracts, and sperm extracts showing different mechanisms according to the present invention have excellent skin barrier recovery rate, high electrical conductivity of skin, It has excellent moisturizing effect, excellent anti-inflammatory effect, and excellent anti-irritant effect when irradiated with UV light, so it has excellent stimulating alleviation effect when applied to human body, and the cosmetic composition containing it as an active ingredient has excellent stability, It has excellent skin penetration and maximizes the effectiveness of the active ingredient, which shows excellent effects on skin protection.

Claims (9)

A skin irritation complex that stabilizes ceramide, mung bean extract, birch extract, and rume extract with a nanoliposome as an active ingredient in a mass ratio of 1: 1: 1. The skin irritation complex according to claim 1, wherein the total content of the active ingredient is contained in an amount of 0.1-20.0% by weight based on the total weight of the nanoliposomes. The method of claim 1, wherein the nanoliposomes are skin irritation complex, characterized in that which is made of a high pressure homogenizer. The method of claim 1, wherein the average particle diameter of the nanoliposomes are skin irritation complex, characterized in that 10-500nm. Cosmetic composition containing the skin irritation complex of any one of Claims 1-4. The cosmetic composition according to claim 5, wherein the composition is for skin irritation relief. The cosmetic composition according to claim 6, wherein the composition is for restoring skin barrier, enhancing skin moisturizing or inhibiting skin erythema formation. The cosmetic composition according to claim 5, wherein the skin irritation complex is contained in an amount of 0.01-20.0 wt% based on the total weight of the composition. 6. The group of claim 5 wherein said composition is comprised of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, surfactant-containing cleansing, oils, powder foundations, emulsion foundations, wax foundations and sprays. Cosmetic composition, characterized in that it has a formulation selected from.