KR100794519B1 - Cosmetic composition comprising bud complex extracts - Google Patents

Abstract

A cosmetic composition comprising bud complex extracts is provided to remove free radicals, inhibit oxidation and inflammation, improve atopic dermatitis and restoration of damaged skin barrier, and enhance skin-moisturizing and skin irritation-alleviating effects by increasing skin permeability with stabilized nanoliposomes. A cosmetic composition for moisturizing the skin or alleviating skin irritation comprises the extracts of bud complex including broccoli, clover, radish and alfalfa which are stabilized by nanoliposomes having average particle diameter of 30-70 nm, and is formulated as skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, soap, shampoo, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, emulsion, press powder, loose powder or eye shadow, wherein the bud complex extracts are prepared by extracting the bud complex with water, C1-C4 lower alcohol or a mixed solvent thereof and are contained in the amount of 0.1-30.0 wt.% based on the total weight of nanoliposomes.

Description

Cosmetic composition containing bud complex extracts {Cosmetic composition comprising bud complex extracts}

The present invention relates to a cosmetic composition containing a bud complex extract (Bud Complex Extract), and more particularly to a cosmetic composition containing a nanoliposome stabilized bud complex extract as an active ingredient, free radical scavenging action, Antioxidant, anti-inflammatory action as well as the skin dryness of atopic dermatitis and recovery of damaged skin barriers, such as when used in infant cosmetics has excellent moisturizing effect and skin irritation relief effect.

The skin is an organ that is in constant contact with the external environment. Its main function is to act as a protective barrier against water loss. The stratum corneum of the epidermis is located on the outermost side of the skin and suppresses the loss of moisture and electrolytes out of the skin, thereby preventing the drying of the skin and providing a normal biochemical metabolism of the skin. In addition, it plays an important role in protecting the human body from external physical damage and chemicals or preventing the invasion of bacteria and viruses into the skin.

Moisture acts to transport various bioactive substances and keeps the skin soft and moist. Of these, the moisture level of the stratum corneum is important, and the stratum corneum of the epidermis contains 10-20% of moisture, which not only prevents evaporation of moisture but also prevents excess moisture from penetrating from the outside. do. For example, polyester fibers contain about 0.4% moisture and cotton 8.5% moisture, while the stratum corneum contains a large amount of moisture and has the unique power to prevent moisture from flying away. This is similar to a structure in which a plastic wrap that does not pass water is wrapped in a sponge drenched in water. That is, it can be considered that a thin oil film is wrapped on the surface of the stratum corneum to prevent evaporation of moisture. This thin oil film is called a natural protective film. Natural protective film is a thin film that covers the surface of the skin and consists of sebum from sebaceous glands and sweat from cold glands. Natural protective film is a water-in-oil (W / O) type emulsified state in which water is partially mixed with oil in a normal state, but when sweating, the sweat evaporates quickly to maintain a constant body temperature. It becomes an emulsified state. After all, the sebaceous membrane, which greatly affects the appearance of the skin surface, is making a reversible transition between water-in-oil and water-in-water. In other words, the natural protective film reversibly converts the water-in-oil type or the oil-in-water type according to the condition of the skin and contributes to moisture control.

Natural Moisturizing Factor (NMF) and Stratum Corneum Lipid (NMF) help to maintain the moisture balance in the horny layer. These are present in the stratum corneum to hold and retain moisture. On the other hand, the water-soluble components of the stratum corneum collectively referred to as a natural moisturizing factor, but the stratum corneum exhibits flexibility is due to the action of these natural moisturizing factors and thus can maintain proper moisture. When the skin's moisturizing power is lowered, it becomes less flexible and dries, and the resistance is lowered, making it sensitive to irritation and susceptible to damage.

Baby skin also protects the body from germs and releases sweat and heat to regulate body temperature. In addition, the lungs, internal organs, kidneys to help function of the body to remove the waste, but there is a difference from adults. This is why many babies have sweat bands because their skin, as well as other major organs such as sweat glands, are still immature and lack the body's ability to control their temperature and control their temperature. . In addition, it easily loses moisture and becomes dry and thus susceptible to damage. In this way, the baby's skin is the same as the adult but its appearance is not enough in terms of function, and it is only about 3 years old to become the thickness of adult skin, so the baby's water retention is a basic requirement for maintaining healthy skin. It is important. In addition, soft baby skin is thin and soft, sensitive to external irritation and bacterial infections, and penetrating ability is much faster than adults, so sweat or bacteria remaining on the skin penetrates into the skin again, which causes the skin to crush. Skin problems may occur. In other words, baby's skin is soft, delicate and sensitive, so skin troubles occur even on small external stimuli, and it is easy to react sensitively to small stimuli to raw materials used in skin care products. It is also important to select and use raw materials that can alleviate the risk. In addition, in order to prevent skin troubles, it is most important to keep the baby's skin moist, relieve irritation, and keep it clean by using a gentle baby skin care product.

Like this, baby's skin tends to dry when it's not well moisturized, and this dry skin responds to very small irritation and can develop into atopic dermatitis. Therefore, the weaker and sensitive skin, the more moisturizing and stimulating function is needed. I will.

Looking at the prior published patent of each extract used in the present invention, broccoli was used for anti-allergic and anti-inflammatory (Japanese Patent Laid-Open No. 2003-267880), clover moisturizing (Japanese Patent Laid-Open No. 2001-122730) Was used for anti-allergic use (Japanese Patent Laid-Open No. 2000-169382). In addition, alpha wave was used for anti-aging (US Patent Publication No. 2005-0208160), lipase inhibitor (JP Patent Publication No. 2003-026585), whitening (JP Patent Publication No. 5-040884).

However, the cosmetic composition containing the sprout complex extract mixed with the sprout extract of each extract has not been applied so far, and also has not been applied to the cosmetic by collecting the sprout complex extract in nano liposomes.

The purpose of the present invention is to reveal that the sprout complex extract has an excellent effect in moisturizing, free radical scavenging action, antioxidant action, anti-inflammatory action as well as improving the resilience of atopic dermatitis and dry skin and damaged skin barrier The present invention has been completed.

In addition, to enhance the skin penetration effect of the sprout complex extract, it is possible to stably deliver the biologically active ingredients in the pharmaceutical, cosmetics and food fields, using the nano-liposomes widely used to maximize the skin penetration effect sprout extract complex Stabilization completed the present invention.

The present invention provides a cosmetic composition containing a sprout complex extract. The sprout complex extract is a polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof, most preferably, by adding ethanol to extract Characterized in that.

In addition, the composition is effective in the skin moisturizing or skin irritation, characterized in that it is utilized to improve the skin condition.

In addition, the composition is characterized in that it is utilized as a cosmetic for children.

The sprout vegetable used in the sprout extract used as an active ingredient of the present invention refers to a sprout that has sprouted for about a week, and the sprout composite extract refers to a mixture of sprout vegetables such as broccoli, clover, radish, and alpha waves. The mixing ratio of each sprout extract is not particularly limited.

In addition, the sprout vegetable is characterized in that it is grown organically. Organic means farming using only natural materials such as organic matter, natural ores and microorganisms without using any synthetic chemicals such as all chemical fertilizers, organic synthetic pesticides (pesticides, growth regulators, herbicides) and livestock feed additives. Therefore, it is possible to eliminate various side effects caused by the use of chemical fertilizers and pesticides, it is a natural and healthy vegetables.

Broccoli (Brassica oleracea var. Italica), a sprout vegetable, is a perennial plant of the dicotyledonous poppy, Mustardaceae, a cabbage native to the Mediterranean or Asia Minor, 50-80 cm high. Branches grow straight and grow, with dense green flowers on the central axis and the ends of the branches. The stem grows longer and the bud is 12-15cm in diameter. Broccoli sprouts contain a large amount of anticancer substance called sulforaphane, which is also attracting attention as a functional food. It also contains vitamins B and C.

In addition, clover (Trifolium repens) is a perennial herb of the dicotyledonous plant Rosaceae, 20-20 cm high. Hairless throughout the abandonment, roots and sparse leaves running from stem nodes that lie on the ground. The leaf is a leaf with 3 small leaves, and the petiole is 5-15cm long and long. There are three small leaves, but some have four. The heart is upside down, 15-25mm long, and 10-25mm butterfly. The end is round or concave with fine teeth on the edge. Jaw leaves are oval-shaped, with a sharp tip. Clover buds contain vitamins and minerals that help clear blood.

In addition, radish (Raphanus sativus) is three vegetables with cabbage and red pepper. The upper part of the large columnar root is stalked, but the boundary is not clear. Root leaves are quilled leaf once (一 回 羽狀 複葉) and displaced. Hairs have the largest split pieces; A stalk is 1m long and grows on the next branch. Underneath it grows the inflorescence. Radish sprouts (sunless, Dandy) are rich in vitamins and act as amylases to digest starch.

Finally, alfa (Medicago sativa) is a perennial herb of dicotyledonous rosewood legume, whose main stem grows straight up to 30 ~ 90cm and branches. The leaves are shifted and the leaves are 3 small leaves. Small leaves are long oval or basso, blunt or recessed in tip, sawtooth at edge. Alpha sprouts are high in fiber and contain amino acids such as arginine, lysine and threonine. It is also rich in vitamins A, K, and U.

Sprout complex extract according to the present invention can be prepared as follows.

Sprout vegetables are washed thoroughly with water and dried in the shade. Dry sprout vegetables are placed in the extraction container and extracted for a certain period of time at an appropriate temperature in an appropriate amount of extraction solvent. The extract can be filtered and concentrated using a vacuum rotary evaporator or the like.

As the extraction solvent, a polar solvent selected from water (purified water), a lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof may be used, and preferably ethanol is used.

The amount of the extraction solvent is 2 to 10 times the dry weight of the herbal medicine. The extraction method may be an extraction method such as water bath extraction, reflux cooling extraction, ultrasonic extraction, etc., preferably may be extracted 1 to 5 times as an extraction method of water bath extraction.

The extraction temperature is 30 ~ 70 ℃, more preferably 40 ~ 60 ℃, most preferably maintained at about 50 ℃ is most effective for the prevention of decay, discoloration and odor.

The extraction time is 5 to 20 hours, preferably 10 hours.

After obtaining the sprout composite extract, the solids are removed using a filter paper, the suspension is centrifuged, and the supernatant can be filtered under reduced pressure.

The filtered extract can be concentrated under reduced pressure at 20 ~ 100 ℃, preferably 50 ~ 70 ℃ with a vacuum rotary concentrator.

Sprout complex extract according to the present invention is effective in reducing skin moisturizing and irritation and is used to improve skin condition. The cosmetic composition of the present invention improves the moisturization of the skin by increasing the natural moisturizing factor and is excellent in stability in the formulation. This fact is specified in the following examples.

According to a preferred embodiment of the present invention, the sprout composite extract may contain 0.1 to 30.0% by weight relative to the total weight of the nanoliposomes.

As used herein, the term "nanoliposomes" refers to liposomes having an average particle diameter of 1 to 100 nm as having a conventional liposome form.

 According to a preferred embodiment of the present invention, the average particle diameter of the nanoliposomes is 30 to 70 nm. When the average particle diameter of the nanoliposomes exceeds 70 nm, the improvement of skin penetration and the improvement of formulation stability are very weak among the technical effects to be achieved in the present invention.

The nanoliposomes of the present invention are prepared by a mixture comprising polyols, surfactants, phospholipids, fatty acids and water, and are characterized by not using monohydric alcohols (eg ethanol).

The polyol used in the nanoliposome of the present invention is not particularly limited, and preferably, propylene glycol, dipropylene glycol, 1,3-butylene glycol, glycerin, methyl propane diol, isoprene glycol, pentylene glycol, erythritol, xyitol and Sorbitol, most preferably propylene glycol. The amount used is 10 to 80% by weight, preferably 30 to 70% by weight based on the total weight of the nanoliposomes.

Surfactants used in the preparation of the nanoliposomes of the present invention may be any known in the art, for example, anionic surfactants, cationic surfactants, amphoteric surfactants and nonionic surfactants may be used. , Preferably anionic surfactants and nonionic surfactants are used. Specific examples of anionic surfactants include alkylacylglutamate, alkylphosphates, alkyllactylates, dialkylphosphates and trialkylphosphates. Specific examples of nonionic surfactants include alkoxylated alkyl ethers, alkoxylated alkyl esters, alkylpolyglycosides, polyglyceryl esters and sugar esters. Most preferably, polysorbates belonging to the nonionic surfactant are used. The amount of the surfactant is generally 0.1 to 10% by weight, preferably 0.5 to 5.0% by weight based on the total weight of the nanoliposomes.

Phospholipids, another component used in the preparation of the nanoliposomes of the present invention, are used as amphoteric lipids, including natural phospholipids (eg, egg yolk lecithin or soy lecithin, sphingomyelin) and synthetic phospholipids (eg dipalmitoyl). Phosphatidylcholine or hydrogenated lecithin), preferably lecithin. More preferably, the lecithin is naturally occurring unsaturated lecithin or saturated lecithin extracted from soybean or egg yolk. Typically, lecithin derived from nature has 23-95% phosphatidyl choline and 20% or less ethanolamine in phosphadidyl. In the preparation of the nanoliposomes of the present invention, the amount of lecithin used is 0.5 to 20% by weight, preferably 2 to 8% by weight, based on the total weight of the nanoliposomes.

The fatty acids used in the preparation of the nanoliposomes of the present invention are higher fatty acids, preferably saturated or unsaturated fatty acids of the C12-22 alkyl chain, such as lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid and linoleic acid. Include. The amount used is 0.05 to 3.0% by weight, preferably 0.1 to 1.0% by weight based on the total weight of nanoliposomes.

Water used in the preparation of the nanoliposomes of the present invention is generally deionized distilled water, the amount of which is 5.0 to 40% by weight based on the total weight of the nanoliposomes.

The preparation of nanoliposomes can be accomplished through various methods known in the art, but most preferably, a mixture comprising the above components is applied to a high pressure homogenizer. The preparation of nanoliposomes by the high pressure homogenizer can be carried out under various conditions (eg, pressure, frequency, etc.) according to the desired particle size, preferably 1 to 5 times high pressure homogenizer under 600 to 1200 bar pressure. Nanoliposomes are prepared by passing through.

The nanoliposome system thus prepared has the advantage of maximizing skin penetration effect by dissolving various types of poorly soluble substances and stabilizing unstable substances.

The components included in the cosmetic composition of the present invention include components commonly used in cosmetic compositions in addition to nanoliposomes including organic sprout complex extracts as active ingredients, and include, for example, conventional agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. Phosphorus aids, and carriers.

The composition of the present invention may contain one or more active ingredients exhibiting the same or similar functions in addition to the organic sprout composite extract.

Cosmetics prepared with a cosmetic composition containing the bud complex extract of the present invention as an active ingredient can be prepared in the form of a general emulsion formulation and solubilized formulation. Cosmetics of the emulsified formulations include nutrient cosmetics, creams, essences, etc., and cosmetics of the solubilized formulations are flexible cosmetics. In addition to cosmetics containing the bud complex extract of the present invention, by containing a dermatologically acceptable medium or base may be prepared in the form of adjuvants that can be topically or systemically applied commonly used in the field of dermatology.

Suitable cosmetic formulations include, for example, emulsions, suspensions, microemulsions, microcapsules, microgranules or ionics (liposomes), nonionics obtained by dispersing an oil phase in a solution, gel, solid or pasty anhydrous product, aqueous phase. It may be provided in the form of a vesicle dispersant, in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.

In addition, the cosmetic composition of the present invention, in addition to the sprout complex extract, fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, Commonly used in water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic or dermatological fields, such as any other ingredients used. In addition, the above components may be introduced in an amount generally used in the dermatology field.

Examples of products to which the cosmetic composition of the present invention may be added include, for example, cosmetics such as astringent cosmetics, soft cosmetics, nourishing cosmetics, various creams, essences, packs, foundations, and the like, cleansing agents, soaps, treatments, and essences. Etc.

Specific formulations of the cosmetic composition of the present invention include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition essence, pack, Formulations such as soaps, shampoos, cleansing foams, cleansing lotions, cleansing creams, body lotions, body cleansers, emulsions, press powders, loose powders, eye shadows and the like.

Hereinafter, preferred examples and experimental examples are presented to help understand the present invention. However, the following Examples and Experimental Examples are provided only to more easily understand the present invention, and the contents of the present invention are not limited by the Examples.

Preparation Example 1 Preparation of Sprout Complex Extract

Each 50 g of broccoli, clover, radish, and alpha wave sprout mixtures were finely crushed in a crusher, immersed in 200 ml of 100% ethanol, and warmed in a 50 ° C. water bath for 10 hours. The obtained extract was cooled to room temperature and filtered through a filter paper to give a filtrate. Ethanol was evaporated from the filtrate using nitrogen, concentrated, and then dissolved in 10% ethanol to prepare an extract.

Experimental Example 1 Antioxidant Effect

In order to confirm the antioxidant efficacy, a free radical 1,1-diphenyl-2-picrylhydrazyl (1,1-Diphenyl-2-picrylhydrazyl; hereinafter referred to as DPPH) was used. DPPH was used by dissolving in ethanol to 2 × 10 ^-4 M concentration. 0.15 ml of DPPH (1.5 ppm-30 ppm) or vitamin C (1000 ppm-30 ppm) of the preparation sample 1, respectively, and 1.35 ml of distilled water were added and reacted at room temperature for 30 minutes, and then the absorbance was measured at 520 nm. For DPPH scavenging effect, IC ₅₀ (50% Inhibitory activity) was compared with respect to a sample having a DPPH radical scavenging activity value of 50% or more calculated using Equation 1 below.

DPPH radical scavenging activity (%) = {(A control-B control)-(A sample-B sample)} / {(A control -B control)} × 100

A control: Absorbance of control group without sample

A sample: Absorbance of reaction group to which sample is added

B control: Absorbance of control group without DPPH

B sample: Absorbance of reaction group without DPPH

As can be seen from Table 1 below, the free radical scavenging effect using DDPH, that is, the antioxidant effect was expressed as an IC ₅₀ value, and the sprout complex extract of Preparation Example 1 showed an excellent antioxidant effect compared to vitamin C having excellent antioxidant effect. gave.

Sample (µg / mL) IC ₅₀ (μg / ml) Vitamin C 9.37 Sprout Complex Extract 8.25

Preparation Example 2 Preparation of Nanoliposomes Containing Sprout Complex Extract

The nanoliposome was added to phase A in the composition of Table 2 below, wetted uniformly, and then phase C was added and heated and mixed to 70 to 80 ° C. The solution was passed through a high-pressure homogenizer 2 to 4 times under conditions of 600 to 1000 bar, and then cooled to obtain a nanoliposome that stabilized the organic sprout composite extract having a particle size of 30 to 70 nm in diameter.
Prize ingredient Content (% by weight) A glycerin To 100 B Hydrogenated lecithin 5.0 Floating paraffin 1.0 Macadamia Nut Oil 1.0 Squalane 1.0 Dimethicone 1.0 Isopropyl myristate 1.0 Sodium Stearoyl Lactate 0.5 Polysorbate-80 0.5 Stearic acid 0.5 C Sprout Complex Extract 20.0 Potassium hydroxide Quantity Purified water 5.0

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Experimental Example 2 Anti-inflammatory Effect Evaluation Method-Carrageenan Foot Edema

After administering 10-100 mg / kg of the nanoliposomes stabilized with the sprout composite extract obtained in Preparation Example 1 and the sprout composite extract obtained in Preparation Example 2 to the abdominal cavity of mice, 1 hour, 0.5 ml of 0.1% carrageenan solution was tested. Inflammation was caused by injection into the animal's hind paw. Immediately after carrageenan infusion and 4 hours after infusion, changes in the paw volume of rats were measured. The results are shown in Table 4 below.

% Inhibition Rate = (1-ΔV Treatment / ΔV Control) × 100

(ΔV: change of foot volume)

Dose (mg) % Inhibition rate Preparation Example 1 Preparation Example 2 10 22.6 37.3 30 32.9 49.4 50 51.7 65.9 100 59.6 80.1

Looking at the Table 3, the sprout composite extract showed an anti-inflammatory effect, in particular, it was confirmed that the nanoliposomes stabilized sprout composite extract shows a better anti-inflammatory effect.

Preparation Example 3 Examples and Comparative Examples

Composition containing nanoliposomes stabilized sprout composite extract of the present invention (Example 1), composition containing sprout composite extract not in the form of nanoliposomes (Example 2) and composition containing neither (Comparative Example 1) Was prepared in the composition shown in Table 4.

ingredient Example 1 Example 2 Comparative Example 1 Content (% by weight) Content (% by weight) Content (% by weight) Sprout Complex Extract (Manufacturing Example 1) 2.0 - - Nanoliposomes (Manufacturing Example 2) - 10.0 - A Cetostearyl alcohol 2.0 2.0 2.0 Glyceryl Stearate 1.5 1.5 1.5 Mikey-Crystalline 0.7 0.7 0.7 Squalane 5.0 5.0 5.0 Liquid paraffin 3.0 3.0 3.0 Trioctanoine 5.0 5.0 5.0 Polysorbate 1.2 1.2 1.2 Sorbitan stearate 0.5 0.5 0.5 Tocopherol Acetate 0.2 0.2 0.2 Micromethicone 3.0 3.0 3.0 BHT 0.05 0.05 0.05 B glycerin 4.0 4.0 4.0 1,3-butylene glycol 2.0 2.0 2.0 EDTA-2Na 0.05 0.05 0.05 Purified water To 100 To 100 To 100 Incense, preservative Quantity Quantity Quantity

Experimental Example 3 Skin Penetration Effect

The skin penetrating effect on the cosmetics containing the nanoliposomes stabilized sprout composite extract of the present invention was measured. Dermal Equivalent (DE) was used to measure the effect of skin penetration, and the skin equivalent was 1 × 10 ⁵ cells / ml of normal human fibroblasts / mL (Korea Cell Line Bank). 3mg / ㎖ collagen aqueous solution: 5 × DMEM: 2.2% sodium bicarbonate with a 0.05N sodium hydroxide 200mM Hepes buffer solution containing the 7: 2: 1 was inoculated in the culture medium mixed in, in 5% CO _2, 37 ℃ 7 Incubated for days. It is placed inside the plate separated by the inside and the outside by a membrane made of 3 μm porous polycarbonate, and epidermal Keratinocyte on the surface of Derma Equivalent (DE) where the fibroblasts were cultured. ) Inoculated with 1 X 10 ⁵ cell / ㎖ cells and cultured for 7 days in the inner and outer K-SFM medium containing EGF and BPE.

Afterwards, the medium inside the plate was discarded to allow the air to come into contact with the surface of the cultured cells. The medium was mixed with K-SFM containing 10FBS and DMEM without EGF in the same amount and incubated for 2 weeks. Artificial skin with dermis was obtained. Example 1 and Example 2 of the present invention was applied to the artificial skin tissue of the top layer, and after 4 hours of incubation, 10FBS-containing K-SFM and the amount of water were analyzed by HPLC. The test results are shown in Table 5 below.

Amount of Sprout Complex Extract in Medium (µg / mL) Example 1 Example 2 3.5    35.9

As a result of the experiment, as shown in Table 5, Example 2 containing the nanoliposomes stabilized sprout composite extract of the present invention can be seen that the skin penetration effect is more than 10 times compared to Example 1 using the sprout composite extract as it is. . This is because the average particle size of the nanoliposomes of the present invention is very small to 30 ~ 70 nm is believed to be easy to penetrate the skin.

Experimental Example 4 Moisturizing Effect

Moisturizing effect was measured by the following method using the moisture meter [Corneometer CM 825, Courage + Kazaka] for Example 1 and Example 2.

Twenty women in their 20s were washed several times with water in the upper arm 2 × 2 ㎠ and 8 compartments, and then water was removed without irritation. After 5 minutes, 10 μl of purified water, Example 1, Example 2, and Comparative Example 1 were applied dropwise to each of the two compartments, and the coating was evenly applied. After 10 minutes, five measurements were taken per compartment using a coronometer. Six times at five minute intervals for one compartment, measured by the following equation (3), the data values are expressed as the average value for 20 people. The room temperature was 20 ° C and relative humidity 20%.

Moisture content (%) = Moisture meter after sample application-Moisture meter before sample application

Time (min) Example 1 Example 2 Comparative Example 1 Purified water 10 28 30 29 25 15 16 25 12 7 20 11 21 7 3 25 8 18 2 One 30 7 15 One 0 35 5 14 One 0 40 2 14 0 0

As shown in the results of Table 6, it was confirmed that the sprout composite extract of the nanoliposome form of Example 2 had the most excellent moisturizing effect.

[Experiment 5] Comparison of skin irritation relief effect when applied to the human body

For 20 women in their 20s and 30s, 5% Lactic Acid (L-lactic acid) was added to Examples 1, 2, and Comparative Example 1 used in Experiment 4 to allow 10 days of use. The mitigating effects on the stimuli induced by tic acid were compared. Each morning and evening were used, and then questionnaires were marked for irritation and intensity. Test results were evaluated once a day, and divided into subjective and objective stimuli, and the results are shown in Table 7 below.

Test substance Subjective stimulation Objective stimulation Sum Example 1 15 11 26 Example 2 9 8 17 Comparative Example 1 31 18 49

As can be seen in Table 7, the cosmetic composition containing the sprout complex extract stabilized with the nanoliposomes obtained by Example 2 of the present invention is very excellent in alleviating the skin irritation induced by 5% lactic acid compared to Comparative Example 1 It can be seen.

Experimental Example 6 Effect of Recovery Test after Skin Barrier Damage in Hairless Mice

Acetone was applied as a method of skin barrier damage. When the transepidermal water loss (TEWL) reached 4.0 g / m ² / h by dropping acetone into the embryos of 8-12 week old hairless mice, Examples 1, 2, and Comparative Example 1 were 5 cm ^2. The area was applied. TEWL was measured with Tewameter, an evaporimeter from CK (Courage + Khazaka, Cologne, Germany). After 6 hours of application, TEWL was measured to evaluate the extent to which TEWL was reduced to evaluate the extent to which skin barrier function was restored. The recovery rate used for the efficacy evaluation was calculated as shown in Equation 4 below and the results are shown in Table 8.

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Br (Barrier Recovery) = (1-(B t = 6-Bt = 0) / (Bt = d -Bt = 0)) × 100

      Bt = 6: TEWL measured 6 hours after skin barrier injury

      Bt = 0: TEWL measurement before skin barrier injury

      Bt = d: TEWL measurement immediately after skin barrier damage

Test substance % Recovery from initial value after 6 hours Example 1 48.2 Example 2 75.3 Comparative Example 1 2.3

Looking at the results of Table 8, the treatment of containing the sprout complex extract of Example 1 and the nanoliposomes stabilized in the sprout complex extract of Example 2 both increased the recovery effect after skin barrier damage, in particular, Example 2 Was found to be the most effective.

Experimental Example 7 Experiment for Confirming the Atopic Dermatitis

When applying the skin of Examples 1 and 2 and Comparative Example 1, the following experiment was carried out to confirm the effect of improving atopic healing. Atopic dermatitis was investigated in 30 patients who had atopic dermatitis over 5 years. The subjects were divided into three groups, A, B, and C, each ten, and each group was treated with Example 1, Example 2, and Comparative Example 1 every evening, and then applied to the skin once a day for 60 days to confirm improvement of atopic condition. In order to improve the atopy condition was measured.

Test substance Improve Atopy Condition (persons) (%) Example 2 very good 12 60.0 good 6 30.0 is average One 5.0 so so - - Example 1 very good - - good 12 60.0 is average 6 30.0 so so One 5.0 Comparative Example 1 very good - - good 3 15.0 is average 6 30.0 so so 11 55.0

As shown in Table 9, it was found that the composition containing the nanoliposome stabilized sprout composite extract of Example 2 has the best atopic dermatitis healing effect.

Hereinafter, based on the results of the above experimental example, to present a cosmetic composition containing a nanoliposome stabilized sprout composite extract of the present invention. However, the composition of the present invention is not intended to be limited to the following formulation examples.

Formulation Example 1 Flexible Lotion

       Formulation example of a flexible lotion containing nanoliposomes stabilized sprout composite extract was prepared as shown in Table 10.

Raw material Content (% by weight) Nanoliposome Stabilized Sprout Complex Extract 1.00 1,3-butylene glycol 1.00 Disodium ID 0.05 Allantoin 0.10 Dipotassium glycylizate 0.05 Citrix Acid 0.01 Sodium citrate 0.02 Glyceres-26 1.00 Arbutin 2.00 Hydrogenated Castor Oil 1.00 ethanol 30.00 Preservative a very small amount coloring agent a very small amount Flavor a very small amount Purified water Remaining amount

Formulation Example 2 Nutrition Cream

      Formulation example of the nutrient cream containing nanoliposomes stabilized sprout complex extract was prepared as shown in Table 11.

Raw material Content (% by weight) Nanoliposome Stabilized Sprout Complex Extract 2.0 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Plant extracts 3.2 Magnesium Aluminum Silicate 0.3 PEG-40 Stearate 1.2 Stearic acid 2.0 Polysorbate 60 1.5 Lipophilic glyceryl stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 Mineral oil 4.0 Squalane 3.8 Carlylic / Capric Triglycerides 2.8 vegetable oil 1.8 Dimethicone 0.4 Dipotassium glycylizate a very small amount Allantoin a very small amount Sodium hyaluronate a very small amount Tocopheryl Acetate Quantity Triethanolamine Quantity Preservative Quantity Flavor Quantity Purified water Remaining amount

As described above, the cosmetic composition containing the bud complex extract (Bud Complex Extract) of the present invention, more specifically, the cosmetic composition containing the nanoliposome stabilized complex extract is moisturizing, free radical scavenging action, antioxidant activity As well as anti-inflammatory action, it improves skin dryness of atopic dermatitis and resilience of damaged skin barrier, and it has excellent moisturizing effect and skin irritation relieving effect. Increasingly, the skin irritation relief effect is greatly improved, and the stability of the formulation is also greatly improved. In particular, the sprout extract is organically grown is suitable for baby cosmetics.

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Claims (9)

A skin cosmetic composition containing a sprout complex extract consisting of broccoli, clover, radish and alfalfa. The cosmetic composition according to claim 1, wherein the sprout complex extract is stabilized with nanoliposomes. The cosmetic composition according to claim 2, wherein the sprout composite extract is contained in an amount of 0.1 to 30.0 wt% based on the total weight of the nanoliposomes. The cosmetic composition according to claim 2, wherein the nanoliposome has an average particle diameter of 30 to 70 nm. The cosmetic composition according to claim 1, wherein the sprout composite extract is solvent extracted. The cosmetic composition according to claim 5, wherein the solvent extraction is performed by adding a polar solvent selected from water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. The cosmetic composition according to claim 1 or 2, wherein the composition is for moisturizing skin or alleviating skin irritation. delete According to claim 1 or 2, wherein the composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisture cream, hand cream, essence, nutrition Cosmetic composition, characterized in that the formulation is selected from the group consisting of essences, packs, soaps, shampoos, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, latex, press powder, loose powder and eye shadow .