JPS61249363A - Production of yeast extract - Google Patents

Abstract

PURPOSE:To obtain a yeast extract having high glutathione content and useful as a health food, etc., by extracting glutathione from yeast, extracting other cell components from the residue, mixing the extracted components to the glutathione, adjusting to an acidic state, and concentrating, neutralizing and spray-drying the mixture. CONSTITUTION:Yeast (e.g. Saccharomyces cerevisiae IMA 4220) is treated with e.g. an acid to effect the extraction of glutathione. The other cell components are extracted from the extraction residue with e.g. a cell wall lyase. The extracted components are mixed with the glutathione, and the mixture is adjusted to 2-4pH, concentrated, neutralized to 5-6pH and spray-dried to obtain the objective yeast extract.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method for producing a yeast extract, and an object thereof is to provide a method for producing a yeast extract with a high glutathione content. .

Glutathione is a tripeptide whose chemical name is γ-glutamyl cysteinyl glycine, and it participates in the redox system in living organisms and plays important roles such as activation of various enzymes and detoxification.

Yeast extract has traditionally been used as a seasoning, but
Recently, chemical seasonings such as monosodium glutamate have been avoided due to the preference for natural products, and yeast extract, which is a natural product, is being preferred.

The present inventors investigated the efficacy of yeast extract with a high glutathione content and found that it is not only effective as a health food but also extremely effective as a flavor enhancer.

[Conventional technology]

By the way, there are various conventional methods for producing yeast extract, such as enzymatic methods and alkaline extraction methods, but in either case, most of the glutathione in the yeast is lost during the extraction and purification process. was.

[Means for solving problems]

As a result of intensive studies, the present inventors have completed the following method for producing a yeast extract containing high glutathione content.

First, glutathione is extracted from yeast with acid or hot water, and the extract is adjusted to pH 2 to 4 and stored. Meanwhile, the remaining intracellular components (mainly proteins) are extracted from the remaining yeast.
is extracted by a normal method, for example by using a cell wall lytic enzyme, and the extract is mixed with the glutathione extract.
By adjusting H to 5 and spray drying, they have invented a method for obtaining a yeast extract with a high glutathione content without almost reducing the glutathione contained in the yeast.

The types of yeast used in this method are usually baker's yeast,
Usually, it is from the genus Satucharomyces, which is used for brewer's yeast, etc., but the type does not matter.

In addition, the rented land used for culturing yeast usually uses molasses as a sugar source, but there are no particular restrictions on the type or other nutritional sources.

〔Example〕

EXAMPLES Hereinafter, the present invention will be explained in detail with reference to Examples, but the present invention is not limited thereto.

Example 1 Satucharomyces cerevisiae IAM4220 was cultured for 2 days on the following leased land.

Molasses composition (adjusted to 3% sugar concentration) Urea 29g, potassium chloride Log Ammonium phosphorus 23g, magnesium sulfate 10g adjusted to 10Q, culture solution centrifuged and washed to make yeast slurry IQ
(solid content 10% v/v) was obtained. Yeast extracts were produced using the following two methods for each 500 tan.

Method A (conventional method) Add cell wall lytic enzyme (product name Y) to 500 m of yeast slurry.
After adding 0.5 g of L-5 (manufactured by Amano Pharmaceutical Co., Ltd.) and reacting at 45° C. for 24 hours, centrifugation was performed, and the supernatant was concentrated and spray-dried to obtain 35 g of yeast extract. The glutathione content of the obtained yeast extract by the DTNB method was 0.1.
% (relative to bone dry).

Method B (method of the present invention) The yeast slurry 500IIQ was adjusted to pn 2 with hydrochloric acid, stirred and extracted at 60°C for 30 minutes, cooled and centrifuged, and the supernatant was adjusted to pH 2 and cooled. On the other hand, the extracted residual yeast was dispersed with water to 10% v/v, adjusted to pH 6, added 0.5 g of the same cell wall lytic enzyme as in Example A, and incubated at 45°C. After reacting for 24 hours at p
Adjusted to H2.0. After concentrating the mixture, the pH was adjusted to 5 again and spray-dried to obtain 34 g of yeast extract. The glutathione content of the obtained yeast extract was 0.8%.

Example 2 A mutant strain of Yamaba Kokusaku Pulp Co., Ltd. whose parent strain is Satscharomyces cerevisiae IAM4220 was cultured in the same manner as in Example 1, and yeast slurry 900+mff1 (solid content 1
0% w/v) was obtained.

Each 450@α was treated in the same manner as in Example 1 to produce yeast extract.

Method A (conventional method): 30 g of yeast extract was obtained in exactly the same manner as in Example 1. The glutathione content of the obtained yeast extract by the DTNB method was 0.3%.

Method B (method of the present invention) In exactly the same manner as in Example 1, 31 g of yeast extract was obtained. The glutathione content of the obtained yeast extract as determined by the DTNB method was 2.2%.

Claims (1)

[Claims] 1. Glutathione in the bacterial cells is extracted from the yeast slurry in advance, the remaining bacterial components are further extracted from the extraction residue, and the former glutathione extract and the latter bacterial component extract are mixed, Adjust to pH 2-4, and after concentration, pH 5-6
A method for producing yeast extract, which comprises neutralizing and spray-drying the yeast extract.