JPH10191776A - Vegetative propagation method of camphor-wood by cuttage - Google Patents

Abstract

PROBLEM TO BE SOLVED: To avert the danger of physiological disorder and the occurrence of disease injuries, by excess humidity, to improve a graft-root rate and the number of adventitious root formation and to greatly rationalize the production process of nursery stock by pretreating cuttings with an auxin, thereby vegetatively propagating camphor-wood by cuttage. SOLUTION: The cuttings are pretreated with the auxin (the concn. is preferably 40 to 60mg/l), such as indole lactic acid. Further, the cuttings are pretreated with preferably diaminomaleonitrile or 5-methylisoxazole-3-o1 and the vegetative propagation of the camphor-wood by the cuttage is executed. The environment of the propagation bed is preferably maintained at 20 to 35 deg.C air temp., 40 to 65% humidity and light shielding rate of 50 to 75%.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a method for growing camphor tree. More particularly, the present invention relates to a method for growing camphor tree which is pre-treated by immersing cuttings in a plant active substance such as auxin and then cutting.

[0002]

2. Description of the Related Art Camphor trees are terrestrial trees belonging to the genus Cinnamom in the family Camphor Tree. In Japan, they grow in southwestern Honshu, Shikoku, Kyushu and Okinawa. The camphor tree is relatively resistant to pests and insects, and has high adaptability to air pollution. In addition, it is evergreen and has a high appreciation value. Seedlings are produced either directly in the forest or by breeding seeds. When harvesting in the forest, it is extremely difficult to procure a large quantity of seedlings of uniform quality, which directly leads to the destruction of the ecosystem. In seed breeding, on the other hand, although it is possible to prepare a large amount of seedlings, the heterozygosity of the allele is high, so that the traits such as leaf color are not uniform in the seedling population, the production yield is reduced, and However, it has a disadvantage that it lacks consistency when used in such applications. Further, when trying to breed new varieties, it is impossible to obtain uniform seeds and seeds with conventional seed propagation, which hinders breeding. In general, camphor is a difficult plant for cutting, but studies on cutting methods have been made. For example, Japanese Unexamined Patent Publication (Kokai) No. 62-83823 discloses a cutting method in which cuttings of plants belonging to the genus Cinnamom of the family Camphoridae are treated with an auxin solution and then rooted under high humidity conditions. However, no satisfactory vegetative propagation method of camphor tree has yet been found.

[0003]

SUMMARY OF THE INVENTION In view of such circumstances, an object of the present invention is to provide a method for vegetative propagation of camphor tree by cuttings suitable for practical use.

[0004]

Means for Solving the Problems The present invention provides the following (1)-
It has the configuration of (4). (1) A method for vegetative propagation of camphor tree by cuttings, wherein the cuttings are pretreated with auxin. (2) A method for vegetative propagation of camphor tree by cuttings, wherein the cuttings are pretreated with auxin and diaminomaleonitrile. (3) A method for vegetative propagation of camphor tree by cuttings, wherein the cuttings are pretreated with auxin and 5-methylisoxazol-3-ol. (4) The method for vegetative propagation of camphor tree by cuttings according to any one of (1) to (3), wherein the auxin is indolebutyric acid. (5) The environment of the inserted floor is as follows: temperature 20-35 ° C, humidity 40-
The method for vegetative propagation of camphor tree by cuttings according to any one of (1) to (4), wherein the method is maintained at 65% and a light-shielding rate of 50 to 75%.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION The camphor tree in the present invention is a camphor tree of the genus Cinnamom ( Cinnamomum).
camphora ). As long as camphor tree belongs to the genus Cinnamom, any of individuals in a natural population and selected individuals can be a subject of the present invention.

As cuttings, one-year or two-year branches obtained from camphor can be used. Generally, when cutting is used, a young tree with a high growth is preferably used as a parent tree for collecting cuttings.
Older trees are considered unsuitable. However, in the present invention, good results can be obtained not only for young trees but also for cuttings collected from parent trees of 10 years or more. In a permanent tree such as camphor tree, it may take 10 years or more to accurately grasp the characteristics of each individual (such as leaf color, tree vigor, tree shape, etc.). After confirming the characteristics, it is possible to cut it, and after selecting for the purpose of breeding new varieties, it is optimal as a method for growing the individual.

Actually, in order to cultivate new varieties of camphor tree and to multiply them in large quantities for commercial use, it is efficient to perform the following two steps. In the first stage, individuals are grown sufficiently to grow into mature trees in order to determine various characteristics, and individuals having desirable characteristics such as leaf color and tree shape are selected. Cuttings are collected from the excellent individuals obtained here and cut. In the second stage, after cutting the seedlings cut in the first stage for 1 to 3 years, the second cutting is performed using these as parent trees. According to the above method, it is possible to obtain a large amount of genetically uniform seedlings in a short time for selected individuals, that is, new varieties for commercial use.

The auxin used in the present invention is preferably indolebutyric acid (hereinafter abbreviated as IBA), but other auxins such as indoleacetic acid and 2,4-dichlorophenoxyacetic acid can also be used.

[0009] Diaminomaleonitrile (hereinafter referred to as DAMN) used in combination with auxin is known as a non-auxin-based activation promoting substance. Also used in combination with 5-methylisoxazol-3-ol (5-methylli)
soxazol-3-ol) is known as a substance that prophylactically controls wilt disease and stupid seedling disease of rice seedlings in addition to its survival promoting action, and also has a bactericidal action. It is also expected to have an effect of suppressing the growth of fungi in the inside.

[0010] Pretreatment of cuttings is auxin, DAM
This is performed by dipping in an aqueous solution of N, 5-methylisoxazol-3-ol. The concentration of the aqueous solution is 10-20
It is prepared and used at 0 mg / l, preferably at 40-60 mg / l. The immersion time is not particularly limited as long as it is about 3 to 24 hours, but may be 16 to 20 hours from the viewpoint of workability and the like. The work here is to prepare cuttings in the morning, start immersing in the aqueous solution in the evening of the day,
It is a series of work to insert on the floor the next morning.

Any type of soil can be used as long as it has a low level of microbial contamination and an appropriate water retention property. Among them, vermiculite is less likely to be contaminated by microbes, and is excellent in water retention and is optimal. . At this time, it is not necessary to perform soil disinfection or the like in advance.

After cutting, it takes 2 to 4 months in the case of camphor tree until adventitious roots are formed and take root. The environment of the inserted floor during this time is as follows: temperature 20-35 ° C, humidity 40-65.
% And the light-shielding ratio is maintained at 50 to 75%. Conventionally, when cuttings are used, it is necessary to maintain the humidity close to 100% in order to prevent the cuttings from drying, and a method called closed insertion, which covers a cutting floor container with a vinyl film or the like, has been adopted. In the present invention, the humidity may be 40 to 65%, and it can be performed in a natural environment.

Thus, the cutting method of camphor tree of the present invention can be carried out by immersing in the above-mentioned aqueous solution of auxin, DAMN, and 5-methylisoxazol-3-ol, and then cutting.

[0014]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, but it should not be construed that the present invention is limited thereto. Example 1 Preparation of cuttings One-year branches are cut from an adult tree that is more than 10 years old, and the leaves are 4 to
Cuttings were prepared leaving 5 sheets. The cuttings were further cut back in water. Cuttings were carried out in mid-June when the one-year branch was sufficiently mature. Pretreatment It was immersed in an aqueous solution of each composition described in Table 1 for 18 hours. Cuttings Vermiculite was placed in a nursery box (470 × 330 × 70 mm) to form a floor, and 50 cuttings were cut per box. Irrigation management It was carried out appropriately while observing the dry state of the inserted bed. Temperature control During the day, when the temperature exceeded 35 ° C, water was sprayed around the nursery box at noon and 3:00 pm for about 5 minutes to utilize the heat of vaporization of water. Humidity It was managed in a natural environment without covering. At this time, the humidity changed at 40 to 65%. Light environment Two aluminum-coated cold gauze with a porosity of 50% were overlaid to shield light. Illuminance meter (SEKONIC Co., Ltd .: SEKONIC
As a result of measurement using a HANDY LUMI model 246), the light-shielding ratio (illuminance under light-shielding / illuminance under non-light-shielding × 10)
0) was 75%.

[0015]

[Table 1]

Four months after cutting under the above conditions, the rooting state was examined, and the rooting rate (number of roots / number of cuttings × 100) and the number of adventitious roots formed (average value per cutting) were determined.
Here, the success or failure of survival was determined by the presence or absence of adventitious root formation. The number of adventitious roots was determined by measuring the number of primary roots directly emerging from cuttings. Table 1 shows the results.

In the non-added group, rooting and formation of adventitious roots were not observed. It was found that the survival rate was high in the group where IBA and DAMN were used in combination, and the number of adventitious roots was increased in the group where IBA and 5-methylisoxazol-3-ol were used in combination.

Example 2 As a pretreatment, cuttings were added to IBA at a concentration of 50 mg / l to 18
After soaking for a time, cuttings were made. Eight nursery boxes were prepared, and 50 seedlings were cut in each box. Other conditions were the same as in Example 1, and the survival rate and the number of adventitious roots were determined. Table 2 shows the results.

[0019]

[Table 2]

Comparative Example 1 After cutting, the rooting rate and adventitious root formation were carried out in the same manner as in Example 2 except that the humidity was maintained at 90 to 100% by putting it in a closed container (transparent plastic container) and managing it. The number was determined. Table 2 shows the results.

Under the respective control environments of Example 2 and Comparative Example 1, almost the same survival rate and the number of adventitious roots were obtained. This revealed that there was no need to manage under high humidity conditions.

[0022]

As described above, according to the present invention,
Pretreatment with an aqueous solution of auxin and, if desired, DAMN and 5-methylisoxazol-3-ol improves the survival rate and the number of adventitious roots formed, and allows stable propagation of camphor tree by cuttings. Further, since there is no need for hermetic insertion, the danger of causing physiological disorders or diseases due to excessive humidity can be avoided, and the step of acclimatization to the external environment after the rooting can be omitted.
In addition, soil disinfection before cuttings, sterilization of cuttings, meticulous water management, and the need for containers for sealing are not required, so that the production process of seedlings can be greatly streamlined. Further, by providing a stable vegetative propagation method, it can greatly contribute to the growth of new varieties and the propagation of seeds and seedlings.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Mitsumasa Koyama 1-25-19 Wakaoji Temple, Amagasaki City, Hyogo Prefecture Inside Gun Zener Seri Corporation

Claims (5)

[Claims] 1. A method for vegetative propagation of camphor tree by cuttings, wherein the cuttings are pretreated with auxin. 2. A method for vegetative propagation of camphor tree by cuttings, wherein the cuttings are pretreated with auxin and diaminomaleonitrile. 3. A method for vegetative propagation of camphor tree by cuttings, wherein the cuttings are pretreated with auxin and 5-methylisoxazol-3-ol. 4. The method for vegetative propagation of camphor tree by cuttings according to claim 1, wherein the auxin is indolebutyric acid. 5. The cutting according to claim 1, wherein the environment of the cutting floor is maintained at a temperature of 20 to 35 ° C., a humidity of 40 to 65%, and a shading rate of 50 to 75%. The method of vegetative propagation of camphor tree.