JPH0718799B2 - Cell blank processing method for biochemical automatic analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention relates to a cell blank treatment of an automatic biochemical analyzer for measuring the concentration of a specific component by adding a colorimetric reagent or an enzyme reagent to a sample in a reaction tube. It is about the method.

[Conventional technology]

In an automatic biochemical analyzer, the reaction tube is usually washed and drained as shown in FIG. 3, and a sample such as serum is added and stirred therein, and further a colorimetric reagent and an enzyme reagent are added, While stirring and reacting for a fixed time at a constant temperature, the increase or decrease of the absorbance is measured for one or a plurality of wavelengths to convert the concentration of the specific component. After the measurement, the reaction tube is drained, washed, and drained to prepare for the next measurement. The absorbance measured here includes not only the absorbance of the sample but also the regent blank and the cell blank, and therefore a treatment for removing them is necessary.

In the analysis by the conventional biochemical automatic analyzer, first, the device is operated to measure the cell blank before the sample analysis is started, and the cell blank measurement data is stored in the memory. Then, when actually analyzing a sample such as serum, the cell blank of each reaction tube is read from the memory and subtracted to obtain analysis data.

[Problems to be solved by the invention]

In such a biochemical automatic analyzer, the amount of sample and reagent provided for analysis tends to be small, but with such a tendency, there is a problem that the measurement accuracy, which is the basic performance of the device, deteriorates. One of the causes is the problem of processing accuracy of cell blanks. That is, conventionally, the cell blank is measured before starting the analysis of the sample as described above, and the amount is subtracted when the sample is stored in the memory and analyzed, but the measurement of the cell blank is performed every morning. If it is performed once a day, it will change subtly depending on the time of day or the contents of the analyzed sample, etc., and the change deteriorates the measurement accuracy.

The present invention solves the above-mentioned problems, and an object of the present invention is to provide a cell blank processing method for a biochemical automatic analyzer in which the decrease in measurement accuracy due to the cell blank is eliminated.

[Means for solving problems]

To this end, the present invention provides a biochemical automatic analyzer for measuring the concentration of a specific component by adding a reagent to a sample in a reaction tube, stirring and reacting the sample, and having a memory for storing the previous cell blank measurement value. , The cell blank is measured twice before the measurement of the sample, and the central measurement value is adopted as the measurement value of the cell blank from the three measurement values combined with the previous measurement value stored in the memory. It is characterized in that the previous measurement value is rewritten with the value.

[Action]

In the method for treating cell blanks of the automatic biochemical analyzer of the present invention, the cell blanks are sequentially measured twice prior to the measurement of the sample and combined with the previous measurement value stored in the memory.
From the two measured values, the central measured value is adopted as the measured value of the cell blank, and the previous measured value is rewritten with the measured value, so the most reliable measured value of the cell blank can be adopted. The accuracy can be improved.

〔Example〕

 Hereinafter, embodiments will be described with reference to the drawings.

FIG. 1 is a diagram for explaining one embodiment of a cell blank treatment method of an automatic biochemical analyzer according to the present invention, and FIG. 2 is a diagram for explaining a flow of cell blank treatment by a data processing system. .

In FIG. 1, 1 is a sampler, 2 is a reagent line, 3
Is a cleaning system, 4 is a reaction system, 5 is a detection system, 6 is a data processing system, 7 is an operation console, and 8 is a host computer.

The overall configuration of the biochemical automatic analyzer shown in FIG. 1 will be described. When a large number of specimens are prepared for the biochemical automatic analyzer, they are conveyed to the sampler 1 through the conveying system. Then, the required amount of sample is set here, for example, 4
If it has a channel configuration, it is distributed to four channels. On the other hand, reagents corresponding to general biochemical analysis items such as GOT and protein are prepared in the reagent line 2. The reaction system 4 comprises a plurality of reaction tubes, and the sample from the sampler 1 and the reagent line 2
The reaction system from the detection system 5
Absorbance is measured with a colorimeter. The absorbance is sent to the data processing system 6 and converted into a concentration value. In addition, the detection system 5
Then, immediately before the analysis of each reaction tube of the reaction system 4, the cell blank is sequentially measured. The cleaning system 3 is for cleaning the reaction tube with a detergent or water after the measurement of the absorbance of the sample in the detection system 5 is completed. The operation console 7 controls the entire system including each of the above systems, and the host computer 8 receives the concentration value data of the specific component from the data processing system 6 through the operation console 7 and manages the data for each sample. It is a thing.

In the biochemical automatic analyzer of the present invention, the cell blank of the reaction tube is sequentially measured immediately before the analysis. In addition, in this case, the cell blank is measured twice for each reaction tube, the previous data is stored in the memory of the data processing system, and the combined data is processed. In this processing, the median value of the three data is adopted, and when these data are within the predetermined width, the median value is newly stored as data in the memory.

Next, the cell blank determination process of the present invention will be described with reference to FIG.

Now, let us say that the data stored in the memory is A ₁ , the first measurement data is A ₂ , and the second measurement data is A ₃ . Therefore, these three data are compared to find the median value data. For example, when A ₁ <A ₃ <A ₂ , the value of A ₃ is set as the cell blank this time, and this value A ₃ is stored in the memory. That is, here, the data in the memory is rewritten from A ₁ to A ₃ .

Furthermore, an example will be described in which the data in the memory is not always rewritten with the median value, but is rewritten under a predetermined condition.

The range of _three data A ₁ , A ₃ , and A ₂ is B _1, and the range of two data A ₂ and A ₃ , that is, the range of measurement data is B ₂ . Then, the reference absorbance for determining whether or not these ranges are within the appropriate range is α _ABS . This α
_ABS is, for example, about 0.00100 _ABS or about 0.00200 _ABS , and the absorbance is set so as not to affect the data.

Therefore, first, it is checked whether the range B ₂ is smaller than the absorbance α _ABS (step).

In the case of NO, that is, when the range B _{2 of the} measurement data A ₂ and A ₃ is large, an N mark is added (step). This N
The mark is a mark for proceeding to the automatic reinspection system.

If YES, it is further checked whether B ₁ is smaller than α _ABS (step).

In the case of NO here, the N mark is added and the memory data is not rewritten and the data in the memory is set as a cell blank (step →).

If YES, the memory data is rewritten from A ₁ to A ₃ , and A ₃ is set as a cell blank (step →).

In this way, the data in the memory is rewritten by determining whether the values of the cell blank ranges B ₁ and B ₂ are smaller or larger than the absorbance α _ABS . Therefore, abnormal data is eliminated rather than averaging three data, so that the reliability of data can be further improved.

[Effects of the Invention] As is clear from the above description, according to the present invention, the cell blanks are successively measured before the start of analysis, so that the accuracy of analysis can be improved. Moreover, since the data with high reliability is selected by comparing the measured value up to the previous time and the measured value this time, the reliability of the data can be further improved.

[Brief description of drawings]

FIG. 1 is a diagram for explaining one embodiment of a cell blank treatment method for a biochemical automatic analyzer according to the present invention, and FIG. 2 is a diagram for explaining the flow of cell blank treatment by a data processing system. FIG. 3 is a diagram for explaining the measurement sequence of the biochemical automatic analyzer. 1 ... Sampler, 2 ... Reagent line, 3 ... Washing system,
4 ... Reaction system, 5 ... Detection system, 6 ... Data processing system, 7
…… Operation console, 8 …… Host computer.

Claims (2)

[Claims] 1. A biochemical automatic analyzer for measuring a concentration of a specific component by adding a reagent to a sample in a reaction tube, stirring and reacting the sample, and comprising a memory for storing a measured value of a previous cell blank, The cell blank is measured twice before the measurement of the sample, and the central measurement value is adopted as the measurement value of the cell blank from the three measurement values combined with the previous measurement value stored in the memory. A method for processing cell blanks in an automatic biochemical analyzer, characterized by rewriting the previous measurement value with. 2. The measurement value is not adopted when the difference between the measurement values is larger than a predetermined value.
A method for treating a cell blank of the described biochemical automatic analyzer.