JPH07107919A - Composition for improving milk quality and increasing milk production - Google Patents

Abstract

PURPOSE:To provide a composition effective for improving the quality and increasing the quantity of raw milk produced by livestock. CONSTITUTION:The composition for improving the quality and increasing the quantity of raw milk produced by livestock, contains at least one kind of compound selected from tannins including (+)-catechin, (+)-gallocatechin, (+)- gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-)-epigallocatechin gallate, free type theaflavin, theaflavin monogallate A, theaflavin monogallate B and theaflavin digallate.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a composition for improving the milk quality of raw milk produced by livestock and increasing the amount of milk produced.

[0002]

2. Description of the Related Art Raw milk is ranked and traded in terms of milk fat percentage, non-fat solids percentage, cell number, and milk protein percentage. For example, in the case of A rank raw milk, the milk fat ratio is 3.5.
%, Non-fat solid content of 8.5% or more, number of cells of 300,000 or less, milk protein ratio of 3.0% or more are required. Is no longer achieved and raw milk that has not reached the number is penalized. That is, in order to be recognized as A-rank raw milk, it is necessary that the milk fat ratio, the non-fat solid content ratio, the cell count, and the milk protein ratio show a certain value or more. Therefore, the deterioration of milk quality causes a great economic loss to dairy farmers. The same can be said for a decrease in milk yield, which leads to an economic loss due to a decrease in productivity.

Under these circumstances, attempts have been made to improve the quality of milk and increase the amount of milk, focusing on concentrated feed (JP-A-2-145154 and JP-A-2-286).
047).

However, the effect obtained by the above-mentioned method is not sufficient, and further improvement is desired at present.

[0005]

DISCLOSURE OF THE INVENTION The present invention is to provide a composition effective for improving the quality of raw milk produced by livestock and increasing the amount of milk produced.

[0006]

Therefore, the present inventors have
As a result of diligent research on a composition effective for improving the milk quality of livestock milk and increasing milk yield, (+)-catechin, (+)-gallocatechin, (+) which are tannins in tea extract -Gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallocatechin, (-)-epigallocatechin gallate, free theaflavin, theaflavin monogallate A, theaflavin monogallate B For the first time, it was found that compounds such as theaflavin digallate exert the above effects,
The present invention has been completed.

The tea extract used in the present invention refers to a substance containing tannins extracted from tea leaves by using a solvent such as water, alcohol or ethyl acetate. The tannins of the present invention are (+)-catechin, (+)-gallocatechin, (+)-gallocatechin gallate, (-)-epicatechin, (-)-epicatechin gallate, (-)-epigallo. Polyphenol compounds such as catechin, (−)-epigallocatechin gallate, free theaflavin, theaflavin monogallate A, theaflavin monogallate B, theaflavin digallate, etc., and hot water extract of tea such as green tea, black tea and oolong tea However, it is also possible to obtain an ethyl acetate-soluble fraction of water or alcohol extract of tea or an ultrafiltration membrane and a reverse osmosis membrane treatment.
The extract residue, which is discarded in large quantities during the production of beverages such as tea leaves or green tea, oolong tea, and black tea, may be used as it is or after being subjected to a treatment such as crushing, as a feed composition. Further, other raw materials such as persimmon shibu or chemically synthesized products may be used. When the obtained tannins are used in the present invention, they can be used alone, as a mixture of two or more kinds, or even a crude extract containing tannins.

[0008] Since such tannins are contained in large amounts as tea ingredients and are already effective in preventing caries, hyperlipidemia, cancer, etc., they can be used to prevent diseases related to these. Because it is used in many foods, its safety is very high.

When the tea extract is administered to livestock in the present invention, it is usually 0.5 to 50 mg per day in terms of tannins.
/ Kg body weight, more preferably 2 to 20 mg / body weight k
It is g. If the dose is less than 0.5 mg / kg body weight, the effect of the present invention cannot be expected, and if it exceeds 50 mg / kg body weight, digestive microorganisms resident in livestock and poultry due to the antibacterial action of tannins. Have an adverse effect on. The tannins can be represented by the amount of tannin quantified by the official tannin analysis method.

When the product of the present invention is administered to livestock, the product of the present invention may be administered in the form as it is or in the form of a formulation, and further, it may be added to feed and administered. The dosage form or method of use is not particularly limited. Furthermore, when used by adding to feed,
The addition method and the addition timing are not particularly limited.

Livestock to which the product of the present invention is administered includes dairy cows, goats,
Any animal used for producing raw milk such as horses and sheep may be used, and the kind thereof is not particularly limited, but in the dairy industry,
Further, due to the bitterness of tannins, it is particularly preferable to use it for dairy cows from the viewpoint of palatability.

Hereinafter, the present invention will be described in detail with reference to examples, but the present invention is not limited to the examples.

[0013]

【Example】

Example 1 1 kg of green tea was mixed with about 15 liters of water and stirred at 80 ° C.
It was extracted for 3 hours. The extract obtained by filtration was spray-dried to obtain 350 g of a hot water extract of green tea containing tannins having a purity of 25%. The composition of the obtained tannins is
(+)-Catechin 1.2%, (+)-gallocatechin 5.
0%, (+)-gallocatechin gallate 3.9%, (-)
-Epicatechin 2.3%, (-)-epicatechin gallate 1.5%, (-)-epigallocatechin gallate 5.0% and (-)-epigallocatechin gallate 6.1%.

Example 2 200 g of green tea was extracted with 4 liters of hot water at 85 ° C. for 30 minutes while stirring, and the tea leaves were removed by filtration to obtain 2.5 liters of extract. This liquid was passed through an ultrafiltration device (manufactured by DDS, membrane type GR-81PP, molecular weight cutoff 6000) to obtain 2 liters of a passing liquid. To the concentrated residual liquid, 1 liter of water was added and the same operation was performed to obtain 1.2 liters of the passing liquid. Both liquids were combined and concentrated with a reverse osmosis membrane (membrane type HC-50, manufactured by DDS) and freeze-dried to 200 ml to obtain 48.6 g of a hot water extract of tea containing tannins having a purity of 29%. The component composition of the obtained tannins is (+)-catechin 1.4%, (+)-gallocatechin 5.8%, (+)
-Gallocatechin gallate 4.5%, (-)-epicatechin 2.7%, (-)-epicatechin gallate 1.8%,
It was 5.8% of (-)-epigallocatechin and 7.0% of (-)-epigallocatechin gallate.

Example 3 To 350 g of the hot water extract obtained in Example 1, 8 liters of water was added and dissolved, and then 10 liters of hexane and 10 liters of chloroform were sequentially distributed. Ethyl acetate (10 liters) was added to the aqueous layer after partitioning, and the mixture was vigorously stirred and allowed to stand, then the ethyl acetate layer was separated, and the ethyl acetate was distilled off and dried to obtain 70 g of an ethyl acetate-soluble fraction (tannins). 74.5% as a mixture of). The content of total tannins in the ethyl acetate-soluble fraction was 74.5%, and the proportion of each tannin was (+)-catechin 3.5%, (+)-gallocatechin 1
4.8%, (+)-gallocatechin gallate 11.6%,
(-)-Epicatechin 7%, (-)-Epicatechin gallate 4.6%, (-)-Epigallocatechin 15.0% and (-)-Epigallocatechin gallate 18.0%.

Test Example 1 Healthy 30 dairy cows were divided into 3 groups with 10 cows each, and Example 1
The group to which 100 mg of the tannins prepared in 1 was administered per day was grouped as A group, the group to which 60 mg of tannins prepared in Example 1 was administered per group as B group, and the non-administered group as C group were bred for 5 weeks. The tannins were mixed with a normal feed and administered. Then, regarding milk quality improved and milk expressed as an index showing an increase in milk amount, the milk fat ratio, non-fat solid content ratio, cell number, milk protein ratio and milk amount per day are determined in accordance with a conventional method from 0 to 5 weeks after the start of the test. It was measured. The results are shown in Tables 1 to 5
, Respectively. Furthermore, the sensory test of milk was conducted by 15 panelists 3 and 5 weeks after the start of the test.
The results are shown in Table 6.

[0017]

[Table 1]

[0018]

[Table 2]

[0019]

[Table 3]

[0020]

[Table 4]

[0021]

[Table 5]

[0022]

[Table 6]

As can be seen from Tables 1 to 6, milk expressed by cows of group A having 100 mg of tannins administered per day was compared to milk expressed by cows of groups B and C, and the milk fat ratio was An improvement in milk quality and an increase in milk production were observed due to an increase in the non-fat solid content, milk protein ratio and milk production per day, and a decrease in the number of cells. That is, in the group A, the milk fat ratio exceeded 3.5%, the non-fat solid content ratio was 8.5%, and the milk protein ratio was 3.0% within 2 weeks after administration, and it was found that the certification condition as A rank was satisfied. . Furthermore, as is apparent from Table 6 by a sensory test conducted by using 15 panelists, milk expressed from cows administered with 100 mg of tannins of group A has aroma,
It was confirmed that the taste and mouthfeel were good. The same test was conducted using the tannins obtained in Examples 2 and 3, but the same result was obtained. Further, the same test was conducted using tannins derived from black tea and oolong tea, but similar results were obtained.

[0024]

As described above, by administering the product of the present invention, the milk fat ratio of raw milk is 3.5% or more, the non-fat solid content is 8.5% or more, the number of bacteria is 300,000 or less, milk protein Rate 3.0
% Can be secured, and it has a great effect on improving the milk quality of raw milk and increasing the amount of milk, which is a great contribution to the livestock industry.

Claims (3)

[Claims] 1. A composition for improving milk quality and increasing milk yield, which comprises a tea extract. 2. The composition for improving milk quality and increasing milk yield according to claim 1, wherein the tea extract is a tannin. 3. Tannins are (+)-catechin, (+)-
Gallocatechin, (+)-Gallocatechin gallate, (-)
-Epicatechin, (-)-epicatechin gallate,
One or more compounds selected from the group consisting of (−)-epigallocatechin, (−)-epigallocatechin gallate, free theaflavin, theaflavin monogallate A, theaflavin monogallate B, and theaflavin digallate. The composition for improving milk quality and increasing the amount of milk according to claim 1 or 2.