JPH0537241Y2 - - Google Patents
Info
- Publication number
- JPH0537241Y2 JPH0537241Y2 JP1987132314U JP13231487U JPH0537241Y2 JP H0537241 Y2 JPH0537241 Y2 JP H0537241Y2 JP 1987132314 U JP1987132314 U JP 1987132314U JP 13231487 U JP13231487 U JP 13231487U JP H0537241 Y2 JPH0537241 Y2 JP H0537241Y2
- Authority
- JP
- Japan
- Prior art keywords
- support
- electrophoresis
- support membrane
- membrane
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012528 membrane Substances 0.000 claims description 22
- 238000001962 electrophoresis Methods 0.000 claims description 15
- 239000007853 buffer solution Substances 0.000 description 11
- 239000000872 buffer Substances 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 229920002301 cellulose acetate Polymers 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 230000020169 heat generation Effects 0.000 description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Electrostatic Separation (AREA)
Description
【考案の詳細な説明】
本考案は、電気泳動に使用される支持体の取り
扱いを改善する為の器具に関する。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to an apparatus for improving the handling of supports used in electrophoresis.
緩衝液を支持体に含浸せしめ、その両側に電圧
を印加することにより該支持体上の試料を泳動せ
しめる電気泳動装置に於いて、該支持体の取り扱
いは、正確な試料分析を行なう為に、素手での取
り扱いが禁止されている等、慎重かつ丁寧さを必
要とするものであるが、泳動時以下に示す点が、
試料の正確な分析を阻んでいた。即ち、緩衝液を
含浸した支持体し、電気的に導電性を有するもの
の、同時に電気的抵抗を具備している。この抵抗
は、支持体に電圧を印加した際、発熱の原因とな
る。この熱は、緩衝液又は試料を蒸散させてしま
うことになり、支持体の乾燥を促すことにより泳
動に重大な支障をきたす要因となつた。 In an electrophoresis device in which a support is impregnated with a buffer solution and a voltage is applied to both sides of the support to cause the sample to migrate, the support must be handled in order to perform accurate sample analysis. Although handling with bare hands is prohibited and requires careful and careful handling, the following points should be noted during electrophoresis:
This prevented accurate analysis of the sample. That is, the support is impregnated with a buffer solution and has electrical conductivity, but also electrical resistance. This resistance causes heat generation when voltage is applied to the support. This heat evaporates the buffer solution or the sample, and promotes drying of the support, causing a serious problem in electrophoresis.
本考案の目的は、支持体の発熱により、緩衝液
又は試料の蒸散を防ぐと共に、手間のかかる支持
体の取り扱いをより簡便にすることである。 The purpose of the present invention is to prevent evaporation of a buffer solution or a sample due to heat generated by the support, and to simplify the time-consuming handling of the support.
以下、本考案の実施例を図面を参照して詳細に
説明する。 Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.
第１図、第２図は、本考案の一実施例を示す図
であり、１は支持板でアクリル等からなり、支持
板１両側は一様に突出し、その突出した表面に緻
密で一様に加工成形された接触面３が形成されて
いる。支持板１に於いて接触面３が形成されてい
る側と反対面には、実験者が取り扱いやすいよう
に把持部２が設けられている。 1 and 2 are diagrams showing one embodiment of the present invention. Reference numeral 1 denotes a support plate made of acrylic or the like. Both sides of the support plate 1 protrude uniformly, and the protruding surface is dense and uniform. A contact surface 3 is formed by processing and molding. A grip part 2 is provided on the opposite side of the support plate 1 to the side on which the contact surface 3 is formed so that it can be easily handled by an experimenter.
このような構成を持つ操作蓋１０は、泳動装置
上では、第４図に示すように配置される。まず最
初に第４図の泳動槽４０の構成について説明す
る。 The operation lid 10 having such a configuration is arranged on the electrophoresis device as shown in FIG. 4. First, the configuration of the electrophoresis tank 40 shown in FIG. 4 will be explained.
第４図は、緩衝液を入れた緩衝槽４３を両側に
設け、更に濾紙４１をブリツジとして配置し、濾
紙４１の一端に支持膜３２を乗せた状態を示す。
そして緩衝槽４３底部には、電極４４が付設さ
れ、この電極は、外部に設けた電気泳動装置用電
源４５と導線４６を介して接続されている。 FIG. 4 shows a state in which buffer tanks 43 containing a buffer solution are provided on both sides, a filter paper 41 is further arranged as a bridge, and a support membrane 32 is placed on one end of the filter paper 41.
An electrode 44 is attached to the bottom of the buffer tank 43, and this electrode is connected to an external power source 45 for the electrophoresis device via a conductive wire 46.
本考案の操作蓋１０は、支持膜上に乗せられ、
接触面３は支持膜３２の縁部に接触している。支
持膜３２は、操作蓋１０によつて覆われることに
なる。このことにより、内部に含浸されている緩
衝液及び試料の蒸散を防ぐことが可能となる。 The operating lid 10 of the present invention is placed on a support membrane,
The contact surface 3 is in contact with the edge of the support membrane 32. The support membrane 32 will be covered by the operation lid 10. This makes it possible to prevent the buffer solution and sample impregnated inside from evaporating.
又、支持膜としてセルロースアセテート膜等を
使用する場合、更に次のような実施例を示し得
る。 Further, when a cellulose acetate membrane or the like is used as the support membrane, the following examples may be shown.
緩衝液に浸された支持膜は、本考案操作蓋の接
触面と互いにその表面が密であることから緩衝液
自身の粘着性及び張力により、第３図に示す如
く、粘着結合する。この事実に基づいて支持膜の
取り扱いは以下の通り行なわれる。 Since the supporting membrane immersed in the buffer solution and the contact surface of the operating lid of the present invention are close to each other, the support membrane is adhesively bonded with the buffer solution itself due to its adhesiveness and tension, as shown in FIG. Based on this fact, the support membrane is handled as follows.
予め緩衝液に支持膜を浸しておき、この緩衝液
が充分に浸透した支持膜表面の両側縁部に本考案
操作蓋内の接触面３を当接する。接触面３には、
上述の通り支持膜３２が粘着結合する。 The support membrane is immersed in a buffer solution in advance, and the contact surface 3 in the operating lid of the present invention is brought into contact with both side edges of the support membrane surface into which the buffer solution has sufficiently permeated. On the contact surface 3,
The support membrane 32 is adhesively bonded as described above.
次に、泳動させるべき試料を装置蓋１０に粘着
した支持膜Ａ面に添付する。そしてそのまま第４
図に示した濾紙の一端に操作蓋１０を乗せればよ
いものである。又、泳動後、操作蓋１０を持ち上
げれば、支持膜３２も同様に持ち上がり、次の動
作に容易に移項できる。 Next, the sample to be electrophoresed is attached to the support membrane A surface adhered to the device lid 10. And then the fourth
The operation lid 10 may be placed on one end of the filter paper shown in the figure. Moreover, if the operation lid 10 is lifted after electrophoresis, the support membrane 32 will be lifted as well, allowing for easy transition to the next operation.
支持膜３２を操作蓋１０から剥す場合、操作蓋
と支持膜との接触面に少量の刺激を与えれば容易
に剥すことができる。 When peeling the support film 32 from the operation lid 10, it can be easily peeled off by applying a small amount of stimulation to the contact surface between the operation lid and the support film.
尚、上記実施例では、支持膜をセルロースアセ
テート膜としたが、支持膜表面が緻密で且つ固形
板状体であれば、操作蓋の効果は充分に発揮され
るものである。 In the above embodiment, the support membrane was a cellulose acetate membrane, but if the support membrane surface is dense and solid plate-like, the effect of the operation lid will be fully exhibited.
以上詳述の如く本考案は、泳動時に於いて支持
膜の発熱によつて生ずる緩衝液及び試料の蒸散を
極力おさえることができ、更に支持膜の取り扱い
が簡易になり得ること等の効果を有するものであ
る。 As detailed above, the present invention has the following effects: it is possible to suppress as much as possible the evaporation of the buffer solution and sample caused by the heat generation of the support membrane during electrophoresis, and the handling of the support membrane can be simplified. It is something.
第１図乃至第３図は本考案の一実施例を示す断
面図乃至斜視図、第４図は本考案の一実施例を電
気泳動に使用した場合の一部断面図である。
１……支持体、２……把持部、３……接触面、
３２……支持膜、４０……電気泳動槽、４１……
濾紙、４３……緩衝槽、４４……電極、４５……
電気泳動出力電源、４６……導電線。
1 to 3 are sectional views and perspective views showing one embodiment of the present invention, and FIG. 4 is a partial sectional view of one embodiment of the present invention used for electrophoresis. 1... Support body, 2... Gripping part, 3... Contact surface,
32... Support membrane, 40... Electrophoresis tank, 41...
Filter paper, 43...buffer tank, 44...electrode, 45...
Electrophoresis output power supply, 46... conductive wire.
Claims (1)
を有する支持体、前記支持体の対向する両縁部に
前記電気泳動用支持膜と一様に接触させる為の接
触面を形成し、且つ前記支持体の他の縁部の１部
乃至全部を解放させたことを特徴とする電気泳動
装置用操作蓋。 A support having an area approximately equal to or smaller than that of the support membrane for electrophoresis, a contact surface for uniformly contacting with the support membrane for electrophoresis is formed on both opposing edges of the support, and the An operating lid for an electrophoresis apparatus, characterized in that part or all of the other edge of the support is opened.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1987132314U JPH0537241Y2 (en) | 1987-09-01 | 1987-09-01 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1987132314U JPH0537241Y2 (en) | 1987-09-01 | 1987-09-01 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6438557U JPS6438557U (en) | 1989-03-08 |
| JPH0537241Y2 true JPH0537241Y2 (en) | 1993-09-21 |
Family
ID=31389161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1987132314U Expired - Lifetime JPH0537241Y2 (en) | 1987-09-01 | 1987-09-01 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0537241Y2 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002068951A1 (en) * | 2001-02-28 | 2002-09-06 | Kabushiki Kaisya Advance | Subminiature electrophoresis device |
| JP2006266875A (en) * | 2005-03-24 | 2006-10-05 | Jokoh Co Ltd | Simplified electrophoretic device |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63300952A (en) * | 1987-06-01 | 1988-12-08 | Fuji Photo Film Co Ltd | Cell and apparatus for electrophoresis |
-
1987
- 1987-09-01 JP JP1987132314U patent/JPH0537241Y2/ja not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63300952A (en) * | 1987-06-01 | 1988-12-08 | Fuji Photo Film Co Ltd | Cell and apparatus for electrophoresis |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6438557U (en) | 1989-03-08 |
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