JPS6039805Y2 - Support for electrophoretic diffusion - Google Patents

Description

[Detailed description of the invention] The present invention relates to a support for electrophoretic diffusion used in the field of clinical testing for protein analysis by immunoelectrophoresis using agar gel, and in particular for tracking precipitation reactions during immunodiffusion. The purpose of the present invention is to provide a low-cost support for electrophoretic diffusion suitable for use in various applications.

Conventionally, in immunoelectrophoresis, etc., an agarose gel-like material is used as a support for electrophoresis, and this support is bridged between two electrically insulated calibration buffers containing a buffer solution using a sponge or the like. Generally, a method is adopted in which electrophoresis is performed by arranging the support in a shape and applying electricity to the support.

Alternatively, instead of using a sponge, one end of a piece of filter paper or agarose is brought into contact with the support, and the other end is immersed in a buffer solution to make an electrical connection.

The support is usually made by dissolving agarose powder, pouring it onto a glass plate or plastic film, and solidifying it into a gel-like state.

This support must be provided with a sample injection hole and an antiserum injection groove, but the sample injection hole and antiserum injection groove are prepared by the analyst or the like before the operation.

Therefore, the conventional immunoelectrophoresis method described above has various drawbacks as described below.

(1) Skill is required to prepare the support, the hole for sample injection, and the groove for antiserum injection, and it is difficult to always prepare a uniform support.

(2) Migration unevenness tends to occur due to poor contact when electricity is applied to the support.

(3) It is difficult to adopt a cooling method to prevent heat generation and drying during electrophoresis.

<4) The support easily peels off from the glass plate or plastic film and penetrates into the gap from the sample injection hole or antiserum injection groove, which may prevent accurate electrophoresis and immunodiffusion.

The present invention was developed in view of the above points.The case is made of a flat hollow transparent body with a horizontal flat surface and curved foot-like hanging parts.A gel for migration and diffusion of agar, agarose, etc. Filling with a shaped support By providing a sample injection hole and an antiserum injection groove on the flat upper surface of the waist case and the support, and further opening the lower end surface of the leg-shaped hanging part of the case to construct the support. The above-mentioned drawbacks are eliminated, that is, a uniform support can be created at all times,
In addition, the purpose is to provide a support for electrophoretic diffusion that can prevent uneven electrophoresis due to poor contact when energized, and can also prevent drying of the support, thereby allowing accurate electrophoretic diffusion. That is.

Hereinafter, the configuration of the present invention will be explained based on embodiments shown in the drawings.

Reference numeral 1 denotes a case made of transparent synthetic resin, and the case 1 is formed into a flat hollow shape consisting of a horizontal flat surface 2 and curved leg-like hanging portions 3 connected to both ends of the flat surface 2. It is something that

The inside of the case 1 is filled with a gel-like support 4 for electrophoretic diffusion made of agar gel such as agar or agarose.

The thickness of this gel-like support 4 is approximately several hundred microns to several millimeters depending on the type of measurement item, and is formed to have a uniform thickness within this range.

A sample injection hole 5 and an antiserum injection groove 6 are provided on the upper surface of the flat surface 2 of the case 1 and the support 4 at the same positions, respectively.

This antiserum injection groove 6 is provided in the current direction, that is, from one hanging portion to the other hanging portion.

The lower end surface of the leg-like hanging portion 3 of the case 1 is open, and the gel-like support 4 is directly exposed to the surface.

When storing this electrophoretic diffusion support before use, cover the sample injection hole 5, the antiserum injection groove 6, and the opening at the lower end of the hanging part 3 with a removable thin film to prevent water evaporation. It is desirable to configure the system to prevent this.

To conduct an analysis using the electrophoretic diffusion support of the present invention configured as described above, first peel off the film cover,
A sample is injected into the sample injection hole 5, and as shown in FIG.
The specimen is immersed in a buffer solution tank 8, and electricity is applied through an electrode 9 to perform electrophoresis.

After the electrophoresis is completed, antiserum is injected into the antiserum injection groove 6 to perform immunodiffusion.

Immunodiffusion is carried out in a sealed box for optical measurement with controlled temperature and humidity.
The type and content of the protein can be determined by optically tracing the sedimentation line caused by the antibody reaction.

The above operations can be performed as a series of operations such as sample dispensing, antiserum injection, energization, and optical tracing using an automated device, and a stable support pre-filled with gel-like support is used. By using the body, it is possible to perform test measurements with high precision and no pre-operation required.

Since the present invention is constructed as described above, it does not require any pre-operation to prepare a support, enables stable and highly accurate analysis, and can be manufactured and supplied at relatively low cost. Since the parts other than the sample injection hole and the antiserum injection groove are covered with the case, this has the effect of preventing the support from drying out.

[Brief explanation of the drawing]

FIG. 1 is a perspective view of the support for electrophoretic diffusion of the present invention, FIG. 2 is a partially enlarged cross-sectional view of the support of the present invention, and FIG. 3 is an explanatory view showing the use state of the support of the present invention. 1...Case, 2...Flat surface, 3...
... Leg-shaped hanging part, 4 ... Support body, 5 ...
... Sample injection hole, 6... Antiserum injection groove,
7...Buffer solution, 8...Buffer solution tank, 9.
·····electrode.

Claims (1)

[Scope of utility model registration request] The electrophoretic diffusion of agar, aga-koma, etc. is carried out inside a flat, hollow case made of transparent synthetic resin, which consists of a horizontal flat surface and curved leg-like hanging parts connected to both ends of this flat surface. Fill the case with a gel-like support, and provide a sample injection hole and an antiserum injection groove on the flat upper surface of the case and the support.
Furthermore, the support for electrophoretic diffusion is characterized in that the lower end surface of the leg-like hanging portion of the case is opened so that the support is exposed.