JP4781188B2 - Antiseptic disinfectant and cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant - Google Patents

Description

  The present invention relates to an antiseptic disinfectant and cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant.

  Parabens, benzoic acids, salicylic acids and the like are used as antiseptic fungicides in cosmetics (including quasi-drugs), pharmaceuticals and foods. However, the above-mentioned conventional antiseptic disinfectant has a drawback that the concentration range in use is easily limited because of low safety such as high skin irritation. For example, the use restriction concentration of paraben and benzoate is 1%, and the use restriction concentration of benzoic acid and salicylic acid is 0.2%. In addition, since these antiseptic fungicides are easily influenced by pH, the antiseptic antibacterial effect is poor in stability, and the antibacterial antibacterial effect may be significantly reduced by the combined use with other compounding components such as a surfactant. He also had problems. In recent years, the number of people who have allergic reactions to these antiseptic disinfectants has increased, so the consumer safety has become more and more important. Demand for reduced cosmetics and pharmaceuticals is increasing.

  As technologies capable of reducing conventional antiseptic fungicides, compositions for external use (see Patent Document 1) containing a combination of 1,2-pentanediol and 2-phenoxyethanol, and antiseptic fungicides comprising 1,2-alkanediol and parabens are used. (See Patent Document 2). However, these are intended to improve the effect of the preservative by using a conventional preservative and 1,2-alkanediol together, and are not intended to completely eliminate the conventional preservative itself. Also, it was not intended to enhance the antibacterial activity of 1,2-alkanediol.

  Moreover, as a technique which can eliminate the conventional antiseptic disinfectant, a preservative disinfectant composed of 1,2-alkanediol (see Patent Document 3) and a moisturizing agent for detergency or non-detergent cosmetics composed of 1,2-octanediol A bacteriostatic agent (see Patent Document 4) and the like are disclosed. However, when 1,2-alkanediol such as 1,2-octanediol is used alone as an antiseptic disinfectant, a high blending amount may be required to obtain sufficient effectiveness. Since 2-alkanediol has a peculiar raw material odor, a technology capable of exhibiting a sufficient antiseptic sterilization effect even at a low blending amount is desired.

JP-A-10-53510 JP 11-310506 A JP 11-322591 A JP 2001-48720 A

  The present invention has been made in view of the above prior art, and by using together a 1,5-alkanediol having 5 to 10 carbon atoms and chitosan oligosaccharide, an antibacterial property inherent in 1,2-alkanediol It is an object of the present invention to provide an antiseptic and bactericidal agent capable of enhancing strength, and cosmetics, pharmaceuticals and foods containing the antiseptic and bactericidal agent.

That is, the invention according to claim 1 relates to an antiseptic fungicide comprising a 1,2-alkanediol having 5 to 10 carbon atoms and chitosan oligosaccharide.
The invention according to claim 2 relates to the antiseptic fungicide according to claim 1, wherein the 1,2-alkanediol is 1,2-hexanediol and / or 1,2-octanediol.
The invention according to claim 3 relates to a cosmetic, pharmaceutical product or food comprising the antiseptic fungicide according to claim 1 or 2.

  The antiseptic disinfectant of the present invention has the effect of enhancing the antibacterial activity inherent to 1,2-alkanediol by synergistically enhancing the antibacterial activity. Further, a cosmetic, pharmaceutical product or food containing the antiseptic disinfectant according to the present invention can eliminate the necessity of containing a conventional antiseptic disinfectant such as salicylic acid, benzoic acid, paraben, and the antiseptic disinfectant according to the present invention. Has an excellent antibacterial activity, so that the preservative disinfectant itself can be made into a low blend.

  The antiseptic fungicide according to the present invention contains 1,2-alkanediol and chitosan oligosaccharide as active ingredients.

The first component according to the present invention is 1,2-alkanediol. As the 1,2-alkanediol used, the following formula (1):

R—CH (OH) —CH ₂ —OH (1)
(In the formula, R represents an alkyl group having 3 to 8 carbon atoms.)

A 1,2-alkanediol having 5 to 10 carbon atoms represented by: Specific examples include 1,2-pentanediol, 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, 1,2-nonanediol, and 1,2-decanediol. Of these, 1,2-hexanediol and 1,2-octanediol are preferably used, and 1,2-octanediol is more preferably used. In addition, in this invention, these 1 type can also be used individually or in combination of 2 or more types.

  The second component according to the present invention is chitosan oligosaccharide. Chitosan oligosaccharide can be prepared, for example, by hydrolyzing chitosan obtained by deacetylating chitin with an acid or an enzyme, and has a structure of the following general formula (1).

  Although the polymerization degree of chitosan oligosaccharide used for this invention will not be specifically limited if the effect of this invention is exhibited, The integer n in the said general formula is 0-10, and the thing of 0-4 is more preferable.

  The chitosan oligosaccharide that can be used may remain in the free amine form or may form a salt. Examples of the salt formed include hydrochloride, sulfate, acetate, lactate, citrate, malate, succinate, adipate, gluconate, tartrate, ascorbate and the like. It is done.

  In addition, a commercial item can also be used for chitosan oligosaccharide as it is. As a commercial item which can be used, brand name COS-YS (made by Yaizu Suisan Chemical Co., Ltd.) etc. can be illustrated.

  In the antiseptic disinfectant according to the present invention, the content of the first component and the second component described above is not particularly limited, but is 0.1: 1 to 10: 1 by weight, preferably 0.2. : It mix | blends so that it may become 1-5: 1. When the 1,2-alkanediol, which is the first component, is added in an amount exceeding 10 times the content of the second component, and if the amount is less than 0.1 times the antibacterial activity cannot be expected. It is not preferable.

  Since the antiseptic fungicide according to the present invention contains 1,2-alkanediol as the first component and chitosan oligosaccharide as the second component, as shown in the examples described later, Due to the synergistic effect of this component and the second component, an enhancing action with excellent antibacterial activity is exhibited. Therefore, it is not necessary to blend or blend conventionally used antiseptics such as parabens, benzoic acids, and salicylic acids, and an extremely safe antiseptic can be obtained.

  The antiseptic disinfectant of the present invention can be used in cosmetics, pharmaceuticals, foods and the like. Specifically, facial cleanser, lotion, milky lotion, skin cream, foundation, mascara, nail enamel, lipstick and other skin cosmetics, shampoo, hair treatment, hair growth / hair restorer, hair cream, hair foam, permanent wave agent, etc. Cosmetics for specific hair use such as hair cosmetics, stains and freckles (quasi-drugs), acne medicines, gargles, lozenges, and other foods such as chewing gum, candy, and drinking water Can be used.

  In addition, the antiseptic disinfectant of the present invention exhibits an excellent synergistic antibacterial activity against oral candidiasis bacteria, so it can also be used as an oral cosmetic such as a denture cleaner, dentifrice, mouthwash, etc. Can do.

  When preparing cosmetics, pharmaceuticals or foods using the antiseptic disinfectant of the present invention, in addition to the above-described components, components usually used for cosmetics, pharmaceuticals or foods as long as the effects of the present invention are not impaired. Can be blended as appropriate. For example, in the case of cosmetics (including quasi-drugs) and pharmaceuticals, fats and oils, waxes, higher fatty acids, lower alcohols, higher alcohols, sterols, fatty acid esters, humectants, surfactants, polymer compounds, inorganic pigments And pigments, antioxidants, ultraviolet absorbers, vitamins, astringents, whitening agents, animal and plant extracts, sequestering agents, purified water, and the like. In the case of food, animal and vegetable oils, polysaccharides, sweeteners, colorants, gum bases and the like can be exemplified.

  When the antiseptic disinfectant of the present invention is blended in cosmetics, pharmaceuticals or foods, the blending amount is not particularly limited, but is 0.01 to 20% by weight, preferably 0.05 to 5% by weight in the composition. This is because when the amount is less than 0.01% by weight, the antibacterial effect is inferior, and even if the amount exceeds 20% by weight, no further effect can be expected.

Example 1
(Test bacteria)
Candida albicans IFO1594 (oral candidiasis) was used as a test bacterium.

(Preparation of bacterial solution for inoculation)
As a bacterial solution for inoculation, after culturing at 30 ° C. on an agar medium, it was further transplanted to a bouillon medium and cultured at 30 ° C. A solution obtained by diluting the obtained culture broth with a broth medium to about 10 ⁷ cells / ml was used as an inoculum.

(Preparation of dilution series of test substance)
5, 4-, 3, 2.5, 2.25, 2, 1.75, 1.5, 1.25, 1 w / v% 1,2-octane using 20 w / w% ethyl cellosolve as a diluent solvent A diol solution was prepared. For chitosan oligosaccharide (trade name COS-YS, manufactured by Yaizu Suisan Chemical Co., Ltd.) and 1,2-octanediol and chitosan oligosaccharide equivalent mixture, A dilution series was prepared.

(Measurement of minimum inhibitory concentration (MIC))
For each 1 mL of the dilution series containing the test substance, 9 mL of each agar medium was placed in a petri dish, and the inoculum for the inoculum was streaked to a length of about 1 cm. The culture was performed at 25 ° C., and the presence or absence of the growth of the bacteria after 3 days was determined. At this time, the minimum concentration where no growth was observed was determined as MIC. The results are shown in Table 1.

  In addition, antibacterial power can be evaluated by MIC. When the test substance concentration is low, there is no effect on microorganisms, but growth increases when the concentration is increased. This degree of growth proceeds depending on the concentration, and finally the growth stops. The concentration at this time is expressed as MIC. Therefore, when the concentration is higher than MIC, the microorganisms are killed.

(Dual minimum inhibitory concentration)
Each MIC of the obtained 1,2-octanediol, chitosan oligosaccharide, and a mixture of 1,2-octanediol and chitosan oligosaccharide was plotted against the blending amount of 1,2-octanediol and chitosan oligosaccharide. A binary minimum growth inhibitory concentration diagram was obtained.

  In addition, the effect at the time of mix | blending two types of substances which have antimicrobial property can be determined with a binary minimum growth inhibitory density | concentration. Specifically, when two types of substances having antibacterial properties are blended, the action caused by the substances is roughly classified into a synergistic action, an additive action, and an antagonistic action. The synergistic action is an action in which two drugs act synergistically to further enhance the inherent antibacterial power. The additive action is an action in which the antibacterial power of each drug is combined. Antagonism is an action when one drug counteracts the antibacterial activity of another drug. Then, the method based on the binary minimum growth inhibition concentration diagram is a method of determining from the graph by measuring the MIC for each of the A substance and B substance while changing their ratios, as shown in FIG. According to this, when MIC (point A) in only substance A and MIC (point B) only in substance B are connected, the MIC when both substances are used is on the inner side of this line (point C) is a synergistic effect in which the antibacterial activity is enhanced by the combined use, if it is on the line (point D), if it is an additive action, if it is outside the line (point E), either or both It can be determined that the antagonistic action counteracts the antibacterial activity and decreases the antibacterial activity.

  FIG. 2 shows a binary minimum inhibitory concentration diagram regarding 1,2-octanediol and chitosan oligosaccharide.

(Evaluation of antibacterial effect)
From the results of FIG. 2, it was recognized that the antibacterial activity inherent to 1,2-alkanediol is synergistically enhanced by the combination of 1,2-alkanediol and chitosan oligosaccharide.

Hereinafter, formulation examples of cosmetics and pharmaceuticals containing the antiseptic disinfectant of the present invention will be shown. In addition, a compounding quantity is weight%.
(Formulation example 1: moisturizing cream)
Decaglyceryl monolaurate 1.0
Polyoxyethylene (15) glyceryl monostearate 1.0
Hydrogenated soybean phospholipid 1.0
Stearic acid 4.0
Cetanol 2.0
Behenyl alcohol 2.0
Paraffin 3.0
Squalane 12.0
Jojoba oil 4.0
Methylpolysiloxane 0.2
1,3-butylene glycol 3.0
L-Arginine 0.1
Xanthan gum 0.001
1,2-octanediol 0.25
Chitosan oligosaccharide 0.1
Purified water residue
Total 100.0

(Formulation example 2: hydrophilic ointment)
Ascorbic acid 0.5
Polyoxyethylene cetyl ether 2.0
Hydrogenated soybean phospholipid 1.0
Stearic acid 4.0
Glycerol monostearate 10.0
Liquid paraffin 10.0
Vaseline 4.0
Cetanol 5.0
Propylene glycol 5.0
1,2-octanediol 0.5
Chitosan oligosaccharide 0.2
Purified water residue
Total 100.0

(Formulation Example 3: Beverage)
Glucose liquid sugar 33.0
Grapefruit juice 64.0
1,2-pentanediol 0.5
Chitosan oligosaccharide 0.05
Acidulant appropriate amount
Total 100.0

  The antiseptic disinfectant of the present invention enhances the antibacterial activity inherent to 1,2-alkanediol and has an excellent antibacterial activity, as well as high safety. Therefore, the antiseptic of cosmetics, pharmaceuticals, foods, etc. It can be suitably used as a disinfectant.

  Further, the antiseptic disinfectant of the present invention exhibits an excellent synergistic antibacterial activity against oral candidiasis bacteria, and is therefore suitable as an oral cosmetic such as a denture cleaning agent, dentifrice, mouthwash, etc. Can be used.

It is a figure which shows an example of the method of judging the effect produced by the case where 2 types of substances which have antimicrobial property are mix | blended from binary minimum growth inhibitory density | concentration. 2 is a binary minimum growth inhibition concentration diagram regarding Example 1. FIG.

Claims (3)

  An antiseptic fungicide comprising a 1,2-alkanediol having 5 to 10 carbon atoms and chitosan oligosaccharide.   The antiseptic fungicide according to claim 1, wherein the 1,2-alkanediol is 1,2-hexanediol and / or 1,2-octanediol. A cosmetic, pharmaceutical or food product comprising the antiseptic disinfectant according to claim 1 or 2.