JP4535744B2 - Antiseptic disinfectant and cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant - Google Patents

Description

  The present invention relates to an antiseptic fungicide and cosmetics, pharmaceuticals and foods containing the antiseptic fungicide, and its purpose is to contain 1,2-octanediol and lemongrass extract as active ingredients, An object of the present invention is to provide an antiseptic and bactericidal agent capable of enhancing the antibacterial power inherent to 2-octanediol, and cosmetics, pharmaceuticals and foods containing the antiseptic and bactericidal agent.

  Parabens, benzoic acids, salicylic acids, and the like are used as antiseptic fungicides in cosmetics (including quasi drugs), pharmaceuticals, and foods. However, the above-mentioned conventional antiseptic disinfectant has a drawback that the concentration range in use is easily limited because of low safety such as high skin irritation. For example, the use restriction concentration of paraben and benzoate is 1%, and the use restriction concentration of benzoic acid and salicylic acid is 0.2%. In addition, since these antiseptic fungicides are easily affected by pH, the stability of the antiseptic disinfection effect is poor, and the antibacterial antibacterial effect may be significantly reduced when used in combination with other components such as surfactants. Also had. In recent years, the number of people who have allergic reactions to these antiseptic disinfectants has increased, so the consumer safety has become more and more important. There is an increasing demand for reduced cosmetics, pharmaceuticals and foods.

  Therefore, as a technique capable of reducing the conventional antiseptic disinfectant, an external composition (see Patent Document 1) that is a combination of 1,2-pentanediol and 2-phenoxyethanol, or an antiseptic comprising 1,2-alkanediol and parabens. A disinfectant (see Patent Document 2) is disclosed. However, these are intended to improve the effect of the preservative by using a conventional preservative and 1,2-alkanediol together, and are not intended to completely eliminate the conventional preservative itself. Also, it was not intended to enhance the antibacterial activity of 1,2-alkanediol.

  On the other hand, as a technique that can eliminate the conventional antiseptic disinfectant, an antiseptic disinfectant composed of 1,2-alkanediol (see Patent Document 3) and a moisturizing agent for cleansing or non-detergency cosmetics composed of 1,2-octanediol. A bacteriostatic agent (see Patent Document 4) and the like are disclosed. However, when 1,2-alkanediol such as 1,2-octanediol is used alone as an antiseptic disinfectant, a high blending amount may be required to obtain sufficient effectiveness. Since 2-alkanediol has a peculiar raw material odor, a technology capable of exhibiting a sufficient antiseptic sterilization effect even at a low blending amount is desired.

JP-A-10-53510 JP 11-310506 A Japanese Patent Laid-Open No. 11-322591 JP 2001-48720 A

  The present invention has been made in view of the prior art, and when 1,2-octanediol and lemongrass extract are used together, the antibacterial nature of 1,2-octanediol has a wide variety of bacterial species. It is an object of the present invention to provide an antiseptic / bacterial agent capable of enhancing strength and cosmetics, pharmaceuticals and foods containing the antiseptic / bacterial agent.

That is, the invention according to claim 1 relates to an antiseptic disinfectant characterized by containing 1,2-octanediol and a lemongrass extract.
According to a second aspect of the present invention relates to a cosmetic, characterized in that blended with antiseptic disinfectant according to claim 1 as an antiseptic disinfectant.
The invention according to claim 3 relates to a pharmaceutical comprising the antiseptic disinfectant according to claim 1 as an antiseptic disinfectant.
The invention according to claim 4 relates to a food comprising the antiseptic disinfectant according to claim 1 as an antiseptic disinfectant.

  The antiseptic disinfectant of the present invention has an effect of enhancing the antibacterial activity inherent in 1,2-octanediol. Further, cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant according to the present invention do not need to contain conventional antiseptic disinfectants such as salicylic acid, benzoic acid and parabens, and the antiseptic disinfectant according to the present invention is excellent. Since the antibacterial activity is high, the preservative disinfectant itself can be made low.

  The antiseptic disinfectant according to the present invention contains 1,2-octanediol and lemongrass extract as active ingredients.

  The first component according to the present invention is 1,2-octanediol, and more specifically 1,2-n-octanediol. 1,2-octanediol itself has antibacterial activity, but the antibacterial activity is synergistically enhanced by combining with the second component described later.

  The second component according to the present invention is a lemongrass extract. Lemongrass is a gramineous plant that is the scientific name of Cymbopogon citratus Stapf or C.flexuosus Stapf.

  Lemongrass extract extract can be prepared by extracting each part of lemongrass leaves, roots, stems, etc. alone or appropriately, and extracting with anhydrous or hydrous organic solvent, water, etc. Commercially available lemongrass extract can be used as it is.

  As an extraction solvent, in addition to water, polar organic solvents such as methanol, ethanol, isopropyl alcohol, n-propyl alcohol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol, n-octanol, etc. 1-8 monohydric alcohols, or ethylene glycol, propylene glycol, 1,3-butylene glycol, hexylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, etc. Carbons such as C 2-6 polyhydric alcohols or derivatives thereof, acetone, methyl acetone, ethyl methyl ketone, isobutyl methyl ketone, methyl n-propyl ketone, etc. 3 to 6 ketone or ethyl acetate, esters of 4-5 carbon atoms such as isopropyl acetate, or ethyl ether, isopropyl ether, ethers of 4 to 8 carbon atoms such as n- butyl ether. Nonpolar organic solvents include petroleum ether, aliphatic hydrocarbons having 4 to 8 carbon atoms such as n-butane, n-pentane, n-hexane, and n-octane, carbon tetrachloride, chloroform, Examples thereof include halogenated aliphatic hydrocarbons having 1 to 2 carbon atoms such as dichloroethane and trichloroethylene, and aromatic hydrocarbons having 6 to 7 carbon atoms such as benzene and toluene, and water and ethanol are preferably used.

  In the antiseptic disinfectant according to the present invention, the content of 1,2-octanediol as the first component and the lemongrass extract as the second component is not particularly limited, but 0.1: 1 by weight. -10: 1, preferably 0.2: 1 to 5: 1. If 1,2-octanediol is added in an amount exceeding 10 times the content of the lemongrass extract extract, and less than 0.1 times the amount, it is not preferable because an antibacterial effect cannot be expected.

  Since the antiseptic disinfectant according to the present invention contains 1,2-octanediol as the first component and lemongrass extract as the second component, it is widely used as shown in the examples described later. Due to the synergistic effect of the first component and the second component on the bacterial species, an enhancing action with excellent antibacterial activity is exhibited. Therefore, it is not necessary to blend or blend conventionally used antiseptics such as parabens, benzoic acids, and salicylic acids, and an extremely safe antiseptic can be obtained.

  The antiseptic disinfectant of the present invention can be used by blending it in cosmetics, pharmaceuticals, foods and the like. Specifically, facial cleanser, lotion, milky lotion, skin cream, foundation, mascara, nail enamel, lipstick and other skin cosmetics, shampoo, hair treatment, hair growth / hair restorer, hair cream, hair foam, permanent wave agent, etc. Cosmetics for specific hair use such as hair cosmetics, stains and freckles (quasi-drugs), acne medicines, gargles, lozenges, and other foods such as chewing gum, candy, and drinking water It can be used suitably.

  When preparing cosmetics, pharmaceuticals or foods using the antiseptic disinfectant of the present invention, in addition to the above-described components, components usually used for cosmetics, pharmaceuticals or foods as long as the effects of the present invention are not impaired. Can be blended as appropriate. For example, in the case of cosmetics (including quasi-drugs) and pharmaceuticals, fats and oils, waxes, higher fatty acids, lower alcohols, higher alcohols, sterols, fatty acid esters, humectants, surfactants, polymer compounds, inorganic pigments , Pigments, antioxidants, ultraviolet absorbers, vitamins, astringents, whitening agents, animal and plant extracts, sequestering agents, purified water, and the like. In the case of food, animal and vegetable oils, polysaccharides, sweeteners, colorants, gum bases and the like can be exemplified.

  When the antiseptic disinfectant of the present invention is blended in cosmetics, pharmaceuticals or foods, the blending amount is not particularly limited, but is 0.01 to 20% by weight, preferably 0.05 to 5% by weight in the composition. This is because when the amount is less than 0.01% by weight, the antibacterial effect is inferior, and even if the amount exceeds 20% by weight, no further effect can be expected.

Example 1
(Co-test)
As test bacteria, Escherichia coli IFO3972 (E. coli), Gram-negative bacteria, Staphylococcus aureus IFO13276 (Staphylococcus aureus) as gram-positive bacteria, Candida albicans IFO1594 (oral candidiasis) as yeast, Aspergillus niger IFO9455 as mold ) Was used.

(Preparation of bacterial solution for inoculation)
In the case of Escherichia coli and Staphylococcus aureus, the bacterial solution for inoculation was cultured at 35 ° C. on an agar medium, then transplanted to a bouillon medium and cultured at 35 ° C. A solution obtained by diluting the obtained culture solution to about 10 ⁸ cells / ml with a bouillon medium was used as a bacterial solution for inoculation.
In the case of oral candidiasis, the same culture at 30 ° C. and diluted to about 10 ⁷ cells / ml are used. In the case of black mold, Tween 80 (polyoxyethylene (20) sorbitan mono) is cultured at 25 ° C. (Oleate) What was prepared by suspending spores in 2% physiological saline and preparing about 10 ⁶ cells / ml was used as a bacterial solution for inoculation.

(Preparation of dilution series of test substance)
5, 4-, 3, 2.5, 2.25, 2, 1.75, 1.5, 1.25, 1 w / v% 1,2-octane using 20 w / w% ethyl cellosolve as a diluent solvent A diol solution was prepared.
Moreover, about the lemongrass extract (made by Hasegawa Koryo Co., Ltd.) and 1,2-octanediol and the mixture of equal amounts of lemongrass extract, a dilution series was prepared by doubling a 5 w / v% solution.

(Measurement of minimum inhibitory concentration (MIC))
For each 1 mL of the dilution series containing the test substance, 9 mL of each agar medium was placed in a petri dish, and for each, the inoculum bacterial solution was streaked to a length of about 1 cm. Cultivation was performed at 35 ° C. for Escherichia coli and Staphylococcus aureus, and the presence or absence of bacterial growth after 2 days was determined. Moreover, about yeast and mold, it culture | cultivated at 25 degreeC and the presence or absence of the growth of a microbe after 3 days was determined. At this time, the minimum concentration at which no growth was observed was determined as MIC. The results are shown in Table 1.

  In addition, antibacterial power can be evaluated by MIC. When the test substance concentration is low, there is no effect on microorganisms, but growth increases when the concentration is increased. This degree of growth proceeds depending on the concentration, and finally the growth stops. The concentration at this time is expressed as MIC. Therefore, when the concentration is higher than MIC, the microorganisms are killed.

(Dual minimum inhibitory concentration)
Each MIC of the obtained 1,2-octanediol, lemongrass extract extract, and an equal weight mixture of 1,2-octanediol and lemongrass extract extract is blended with 1,2-octanediol and lemongrass extract extract. And a binary minimum growth inhibitory concentration diagram was obtained. The results are shown in FIGS.
In addition, the effect at the time of mix | blending two types of substances which have antimicrobial property can be determined with a binary minimum growth inhibitory density | concentration. Specifically, when two kinds of substances having antibacterial properties are blended, the action caused by the substances is roughly classified into a synergistic action, an additive action, and an antagonistic action. The synergistic action is an action in which the two drugs act synergistically to further enhance the inherent antibacterial power. The additive action is an action in which the antibacterial powers of the respective drugs are combined. Antagonism is an action when one drug counteracts the antibacterial activity of another drug. Then, the method based on the binary minimum growth inhibition concentration diagram is a method of determining from the graph by measuring the MIC for each of the A substance and the B substance while changing their ratios as shown in FIG. According to this, when MIC (point A) only for substance A and MIC (point B) only for substance B are connected, the MIC when both substances are used is inside this line (point C) is a synergistic effect in which the antibacterial activity is enhanced by the combined use, if it is on the line (point D), if it is an additive action, if it is outside the line (point E), one or both It can be determined that the antagonistic action counteracts the antibacterial activity and decreases the antibacterial activity.

(Evaluation of antibacterial effect)
From the result of FIGS. 1-4, the synergistic effect of antibacterial activity was recognized with respect to a wide variety of bacterial species by the combination of 1,2-octanediol and lemongrass extract.

Hereinafter, formulation examples of cosmetics, pharmaceuticals and foods containing the antiseptic disinfectant of the present invention will be shown. In addition, a compounding quantity is weight%.
(Formulation example 1: moisturizing cream)
Decaglyceryl monolaurate 1.0
Polyoxyethylene (15) glyceryl monostearate 1.0
Hydrogenated soybean phospholipid 1.0
Stearic acid 4.0
Cetanol 2.0
Behenyl alcohol 2.0
Paraffin 3.0
Squalane 12.0
Jojoba oil 4.0
Methyl polysiloxane 0.2
1,3-butylene glycol 3.0
L-Arginine 0.1
Xanthan gum 0.001
1,2-octanediol 0.25
Lemongrass extract 0.1
Purified water residue
Total 100.0

(Formulation example 2: hydrophilic ointment)
Ascorbic acid 0.5
Polyoxyethylene cetyl ether 2.0
Hydrogenated soybean phospholipid 1.0
Stearic acid 4.0
Glycerol monostearate 10.0
Liquid paraffin 10.0
Vaseline 4.0
Cetanol 5.0
Propylene glycol 5.0
1,2-octanediol 0.5
Lemongrass extract 0.2
Purified water residue
Total 100.0

(Formulation Example 3: Beverage)
Ascorbic acid 0.5
Glucose liquid sugar 33.0
Grapefruit juice 64.0
1,2-octanediol 0.5
Lemongrass extract 0.1
Sour seasoning
Total 100.0

  The antiseptic disinfectant of the present invention has excellent antibacterial activity by enhancing the antibacterial activity inherent to 1,2-octanediol against a wide range of bacterial species, and has high safety. It can be suitably used as an antiseptic disinfectant for pharmaceuticals and foods.

2 is a binary minimum growth inhibitory concentration diagram for Escherichia coli of Example 1. FIG. 2 is a binary minimum growth inhibitory concentration diagram for Staphylococcus aureus of Example 1. FIG. 2 is a binary minimum growth inhibitory concentration diagram for oral candidiasis of Example 1. FIG. 2 is a binary minimum growth inhibitory concentration diagram for black mold of Example 1. FIG. It is a figure which shows an example of the method of determining the effect produced by the case where two types of substances having antibacterial properties are blended from the binary minimum growth inhibitory concentration.

Claims (4)

  An antiseptic disinfectant comprising 1,2-octanediol and a lemongrass extract. A cosmetic comprising the antiseptic disinfectant according to claim 1 as an antiseptic disinfectant . A pharmaceutical product comprising the antiseptic disinfectant according to claim 1 as an antiseptic disinfectant. A food comprising the antiseptic disinfectant according to claim 1 as an antiseptic disinfectant.