JP4503402B2 - Unpleasant taste reducing action and unpleasant odor reducing action - Google Patents
Unpleasant taste reducing action and unpleasant odor reducing action Download PDFInfo
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- JP4503402B2 JP4503402B2 JP2004262284A JP2004262284A JP4503402B2 JP 4503402 B2 JP4503402 B2 JP 4503402B2 JP 2004262284 A JP2004262284 A JP 2004262284A JP 2004262284 A JP2004262284 A JP 2004262284A JP 4503402 B2 JP4503402 B2 JP 4503402B2
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- unpleasant
- panose
- taste
- odor
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Description
本発明は、グルコースを構成糖とした三糖類又は/及びその還元物、特に、パノースの新規用途に関する。より詳しくは、該糖質の不快味低減作用及び不快臭低減作用に関する。 The present invention relates to a novel use of a trisaccharide having glucose as a constituent sugar and / or a reduced product thereof, particularly panose. More specifically, the present invention relates to an unpleasant taste reducing action and an unpleasant odor reducing action of the carbohydrate.
一般的に、飲食物は、味覚や嗅覚を伴って、風味が判断されるため、不快味や不快臭は含まれていないことが望ましい。しかしながら、飲食物の中には、天然素材そのものや、加工・調理過程などで副生してしまう物質などによって、不快味や不快臭が含まれてしまうものも多い。 In general, foods and drinks are judged to have a taste and an olfactory sensation, so that it is desirable that they do not contain an unpleasant taste or an unpleasant odor. However, many foods and drinks contain unpleasant tastes and unpleasant odors due to natural materials themselves or substances that are by-produced during processing and cooking processes.
それらの不快味や不快臭を低減する方法として、例えば、サイクロデキストリンを用いて豆乳の青臭さ、エグ味を低減させる方法(特許文献1)、エリスリトールを用いて野菜・果実類類の青臭み、酸味などの抑制方法(特許文献2)、トレハロースを用いて緑色野菜汁の異味・異臭を低減する方法(特許文献3)などが提案されている。
しかしながら、従来の技術では、飲食物のおいしさを損なうことなく、不快味や不快臭を低減させることは難しかった。 However, with the conventional technology, it has been difficult to reduce unpleasant taste and unpleasant odor without impairing the taste of food and drink.
例えば、特許文献1では、サイクロデキストリンによる、豆乳に残存する青臭みや苦渋味のマスキング効果が示されているが、サイクロデキストリン自体が甘味を有するため、豆乳本来の味を変化させてしまうという課題があった。このため、サイクロデキストリンの添加量と飲食物の味との調整が難しく、広く飲食物全般に利用することが難しかった。その他、サイクロデキストリンは、比較的高価であるという問題もある。 For example, Patent Document 1 shows the masking effect of cyclodextrin on the blue odor and bitter taste remaining in soy milk, but since cyclodextrin itself has sweetness, there is a problem of changing the original taste of soy milk. there were. For this reason, adjustment with the addition amount of cyclodextrin and the taste of food and drink is difficult, and it was difficult to utilize widely for food and drink in general. Another problem is that cyclodextrins are relatively expensive.
また、特許文献2では、エリスリトールを添加することによって、野菜・果物類の青臭み、酸味などを抑制ないしマスキングし、風味を改善することが示されているが、エリスリトールには、緩下作用(下痢)があり、多くを添加するのは、好ましくないという課題があった。 Patent Document 2 shows that the addition of erythritol suppresses or masks the blue odor and acidity of vegetables and fruits and improves the flavor, but erythritol has a laxative action ( There was a problem that it was not preferable to add a large amount.
特許文献3のトレハロースに関しても、異味・異臭の低減効果は示されているが、必ずしも充分な効果が得られるとはいえなかった。また、トレハロースを多く添加してしまうと、素材の味が損なわれ、不自然な味となってしまうので、前記と同様、トレハロースの添加量と飲食物の味との調整が難しく、広く飲食物全般に利用することが難しいという課題があった。 Regarding the trehalose of Patent Document 3, the effect of reducing off-flavors and off-flavors has been shown, but it cannot always be said that a sufficient effect can be obtained. In addition, if a large amount of trehalose is added, the taste of the raw material is impaired, resulting in an unnatural taste. As above, it is difficult to adjust the added amount of trehalose and the taste of food and drink, and food and drink are widely used. There was a problem that it was difficult to use in general.
そこで、本発明は、飲食物のおいしさを損なうことなく、不快味・不快臭を低減させる方法を提供することを主な目的とする。 Therefore, the main object of the present invention is to provide a method for reducing unpleasant taste and unpleasant odor without impairing the deliciousness of food and drink.
本発明に係るパノースは、三つのグルコースから構成される分岐オリゴ糖の一つで、α−1,6とα−1,4グルコシド結合を有する三糖類である。今回、本願発明者は、そのパノースに、不快味低減作用、不快臭低減作用があることを新規に見出した。 The panose according to the present invention is one of branched oligosaccharides composed of three glucoses, and is a trisaccharide having α-1,6 and α-1,4 glucoside bonds. This time, this inventor newly discovered that the panose has an unpleasant taste reduction effect and an unpleasant odor reduction effect.
パノースは、前記のとおり、三糖類であり、三つのグルコースが、弓形状に連なったような立体構造を有する(図1、図2参照)。従って、その弓形の内側に不快味・不快臭の原因となる物質を包み込むようにして結合し、不快味・不快臭を低減するものと考えられる。 As mentioned above, panose is a trisaccharide and has a three-dimensional structure in which three glucoses are connected in a bow shape (see FIGS. 1 and 2). Therefore, it is considered that the substance causing the unpleasant taste and unpleasant odor is bound inside the arcuate shape so as to reduce the unpleasant taste and unpleasant odor.
また、発明者は、さらに鋭意研究を重ねた結果、パノース以外のグルコースを構成糖とした三糖類とその還元物にも、一定の不快味低減作用、不快臭低減作用があることをも見い出した。これは、三糖類の大きさ・立体形状が不快味・不快臭の原因となる物質を包み込むのに適しているためと推測できる。 In addition, as a result of further earnest research, the inventor has also found that trisaccharides composed of glucose other than panose and their reduced products also have certain unpleasant taste reducing effects and unpleasant odor reducing actions. . This is presumably because the size and the three-dimensional shape of the trisaccharide are suitable for enveloping substances that cause unpleasant taste and unpleasant odor.
そこで、本発明では、まず、グルコースを構成糖とした三糖類又は/及びその還元物の、不快味低減有効成分又は/及び不快臭低減有効成分としての使用を提供する。また、グルコースを構成糖とした三糖類又は/及びその還元物を不快味又は/及び不快臭低減の有効成分として含有する糖組成物、及び、前記糖組成物を添加した飲食物を提供する。 Therefore, in the present invention, first, the use of a trisaccharide having glucose as a constituent sugar or / and a reduced product thereof as an unpleasant taste reducing effective ingredient or / and an unpleasant odor reducing effective ingredient is provided. The present invention also provides a sugar composition containing a trisaccharide having glucose as a constituent sugar and / or a reduced product thereof as an active ingredient for reducing unpleasant taste or / and unpleasant odor, and a food or drink to which the sugar composition is added.
前記したグルコースを構成糖とした三糖類とその還元物としては、パノースが最も好適であるが、パニトール、マルトトリオース、マルトトリイトール、イソマルトトリオース、イソマルトトリイトールなどを用いてもよい。また、前記糖類を2つ以上含有する糖組成物を用いることもできる。 Panose is most preferable as the trisaccharide having glucose as a constituent sugar and its reduced product, but panitol, maltotriose, maltotriitol, isomaltotriose, isomaltotriitol, etc. may be used. . Moreover, the saccharide | sugar composition containing 2 or more of the said saccharides can also be used.
ここで、本発明に係る不快味には、例えば、苦味、渋み、酸味、エグ味などがあり、本発明に係る不快臭には、例えば、穀物臭、酸臭、青臭さ、ビタミン臭、魚臭、アルコール臭また加熱殺菌などの製造工程で副生する加熱臭、容器臭などがあるが、これらに限定されるわけではなく、飲食物の風味に含まれる異味・異臭を広く含む。 Here, the unpleasant tastes according to the present invention include, for example, bitterness, astringency, sourness, egg taste, and the unpleasant odors according to the present invention include, for example, grain odor, acid odor, blue odor, vitamin odor, fish There are odors, alcohol odors, heated odors by-produced in the production process such as heat sterilization, and container odors.
不快味・不快臭低減に従来利用されている物質は、その物質自体が独特の味・臭いを有している場合が多く、利用できる飲食品分野が限られていた。本発明は、より広い範囲の飲食品に適用できるという有利性がある。 Substances conventionally used for reducing unpleasant taste and unpleasant odor often have a unique taste and smell, and the available food and drink fields are limited. The present invention has an advantage that it can be applied to a wider range of food and drink.
本発明に係るパノースは、膜ろ過などにより、高純度に精製することも可能であるが、デンプンを原料として、複数の酵素反応を用い、パノースを含有した糖組成物として生産してもよい。本発明者らは、前記の通り、パノース以外の三糖類などにも、一定の不快味・不快臭低減作用があることを明らかにした。従って、上記の方法により生産した、パノースを含有する糖組成物も、不快味・不快臭低減の有効成分として用いることができる。 The panose according to the present invention can be purified to high purity by membrane filtration or the like, but may be produced as a sugar composition containing panose using starch as a raw material and a plurality of enzyme reactions. As described above, the present inventors have clarified that trisaccharides other than panose have a certain unpleasant taste / unpleasant odor reducing action. Therefore, the sugar composition containing panose produced by the above method can also be used as an active ingredient for reducing unpleasant taste and unpleasant odor.
パノースを含有する糖組成物を用いることには、パノースの精製工程などを簡略化でき、製造コストを下げることができるという有利性がある。また、パノースを含有する糖組成物は、前記のとおり、比較的簡単に生産できるため、品質の均一な不快味・不快臭低減の有効成分を、安定的に供給できるという有利性がある。 The use of a panose-containing sugar composition has the advantage that the purification process of panose can be simplified and the production cost can be reduced. Moreover, since the sugar composition containing panose can be produced comparatively easily as described above, there is an advantage that an active ingredient having a uniform quality and an unpleasant taste / unpleasant odor can be stably supplied.
パノースを含有する糖組成物は、パノースを20重量%以上含有させることにより、充分な不快味・不快臭低減作用を得ることができる。ここで、糖組成物中のパノース含量は、澱粉糖関連工業分析法に準じ、高速液体クロマトグラフィー法により求めた(食品化学新聞社発行「澱粉糖関連工業分析法」P132〜P137、1991)。 The sugar composition containing panose can obtain a sufficient unpleasant taste / unpleasant odor reducing action by containing 20% by weight or more of panose. Here, the panose content in the sugar composition was determined by a high performance liquid chromatography method according to the starch sugar related industrial analysis method (“Starch sugar related industrial analysis method” issued by Food Chemical Newspaper, P132 to P137, 1991).
パノースを含有する糖組成物は、パノース自体が低甘味であるため、20重量%以上のパノースを含有させることにより、糖組成物全体の甘味も抑えることができる。そのため、パノースを含有する糖組成物を飲食物に添加した場合、飲食物のおいしさを損なうことなく、その飲食物に含まれた不快味・不快臭を低減することができる。 Since the panose itself has a low sweetness, the panose-containing sugar composition can suppress the sweetness of the entire sugar composition by containing 20% by weight or more of panose. Therefore, when the sugar composition containing panose is added to food and drink, the unpleasant taste and unpleasant odor contained in the food and drink can be reduced without impairing the deliciousness of the food and drink.
なお、パノースを含有する糖組成物を用いる場合、この糖組成物におけるパノース含量を低くすると、不快味・不快臭低減作用を得るために、比較的多量の糖組成物を飲食物に添加しなければならなくなるため、飲食物本来のおいしさを損ね、甘味の強い不自然な味になってしまう可能性がある。従って、パノースを含有する糖組成物を用いる場合、糖組成物におけるパノース含量を少なくとも20重量%以上にすることにより、糖組成物全体の飲食物への添加量を比較的少量に抑えることがより好ましい。 When a panose-containing saccharide composition is used, if the panose content in the saccharide composition is lowered, a relatively large amount of saccharide composition must be added to food and drink in order to obtain an unpleasant taste / unpleasant odor reducing action. As a result, the taste and taste of food and drink may be impaired, resulting in a sweet and unnatural taste. Therefore, when using a saccharide composition containing panose, it is more possible to keep the addition amount of the entire saccharide composition to food and drink by making the panose content in the saccharide composition at least 20% by weight or more. preferable.
本発明に係る糖組成物を飲食物に添加する場合、該糖組成物は、液状・固形状のいずれの形態であってもよい。また、固形状の糖組成物を用いる場合、粉末化、顆粒化、ペレット化、など、食品加工の際に分散しやすいように加工されたものを用いてもよい。 When the sugar composition according to the present invention is added to food or drink, the sugar composition may be in any form of liquid or solid. Moreover, when using a solid sugar composition, you may use what was processed so that it might disperse | distribute easily at the time of food processing, such as pulverization, granulation, and pelletization.
本発明に係る糖組成物の添加の時期は、飲食前に添加されていれば、特に限定されない。飲食品の種類によって適した添加時期は異なるが、例えば、飲食品の調製段階に添加してもよいし、卓上甘味料やドレッシングのように飲食直前に添加してもよい。 The timing of adding the sugar composition according to the present invention is not particularly limited as long as it is added before eating and drinking. Although the suitable addition time differs depending on the type of food or drink, for example, it may be added at the preparation stage of food or drink, or may be added immediately before eating or drinking like tabletop sweetener or dressing.
パノースを含有する糖組成物を飲食物に添加した場合、パノースが最終濃度で少なくとも0.2重量%以上含有していれば、不快味・不快臭低減作用効果を得ることができる。パノースを含有する糖組成物は低甘味であり、また、比較的少量の添加により不快味・不快臭を低減できるため、本発明に係る糖組成物には、飲食物のおいしさを損なわずに添加できるという利点がある。 When a sugar composition containing panose is added to food or drink, the effect of reducing unpleasant taste and unpleasant odor can be obtained if the panose contains at least 0.2% by weight or more in the final concentration. Since the sugar composition containing panose has low sweetness and can reduce unpleasant taste and unpleasant odor by adding a relatively small amount, the sugar composition according to the present invention does not impair the taste of food and drink. There is an advantage that it can be added.
本発明の対象となる飲食物とは、不快臭・不快味を有するものの全てを含み、特に限定するものはないが、例えば、穀物類を原料としたもの、野菜・果実類を原料としたもの、穀物・野菜・果実類以外の植物を原料としたもの、魚貝類を原料としたもの、畜産物を原料としたもの、微生物を原料としたもの、及び、加熱殺菌や発酵などの製造工程の際に副生した不快味・不快臭を有するものなどが挙げられる。 The foods and drinks subject to the present invention include all those having unpleasant odors and unpleasant tastes, and are not particularly limited. For example, those made from cereals and those made from vegetables and fruits. Manufacturing processes such as plants made from plants other than grains, vegetables and fruits, fish shellfish, raw materials, microorganisms, and heat sterilization and fermentation And those having an unpleasant taste and unpleasant odor by-produced.
穀物を原料とした飲食物としては、例えば、パン、お好み焼き、たこ焼き、ケーキ、クッキーなどの焼き・蒸し製品、うどん、中華麺、パスタなどの麺製品、ハンバーグ、シュウマイ、餃子、パンなどの大豆タンパク質を利用した加工食品、豆乳飲料、調製豆乳、豆腐、テンペなどの大豆加工飲食物、イソフラボンやサポニンなどの穀類成分含有飲食物・サプリメント・健康補助食品、食酢飲料、黒酢飲料などの酢製品、ビール、日本酒などの醸造酒類、苦味やエグ味のある素材、などが挙げられるが、それらに限定されない。小麦、ライ麦などの麦類、そば、とうもろこし、大豆など、豆類、米類などを原料としたものは、本発明に係る飲食物に包含される。 Foods and drinks made from grains include, for example, baked and steamed products such as bread, okonomiyaki, takoyaki, cakes and cookies, noodle products such as udon, Chinese noodles and pasta, and soy protein such as hamburger, shumai, dumplings and bread. Processed foods, soy milk beverages, soy processed foods such as prepared soy milk, tofu, tempeh, etc., foods / supplements / health supplements containing cereal components such as isoflavones and saponins, vinegar products such as vinegar beverages, black vinegar beverages, Examples include, but are not limited to, beer, brewed sake such as Japanese sake, and materials with bitterness and taste. Wheat, rye and other barley, buckwheat, corn, soybeans and other beans, rice and the like are included in the food and drink according to the present invention.
なお、食酢などの酢製品やビールなどの醸造酒は、原料が穀物のものに特に限定されず、例えば、りんごやブドウを原料とした果実酢や、果実酒なども含まれる。また、ウイスキー、ブランディー、ウォッカ、焼酎などの蒸留酒、醸造酒、蒸留酒、アルコール又は他の成分を混合するリキュール、カクテル、チューハイ、フィズなどのアルコール飲料なども含まれる。 The vinegar products such as vinegar and the brewed liquor such as beer are not particularly limited to cereals, and examples thereof include fruit vinegar made from apples and grapes, and fruit wine. Also included are distilled liquors such as whiskey, brandy, vodka and shochu, brewed liquor, distilled liquor, alcoholic beverages such as liqueurs, cocktails, Chuhai and Fizz mixed with alcohol or other ingredients.
野菜・果実類を原料とした飲食物としては、例えば、野菜ジュース、野菜ピューレ、野菜スープ、青汁、果汁飲料、果実酒、野菜・果実類に含有する特殊成分を強化した飲食物・サプリメントなどが挙げられるが、それらに限定されない。野菜・果実類を原料としたもの、例えば、トマト、ニンジン、ピーマン、パセリ、セロリ、ホウレン草、モロヘイヤ、ケール、ノニ、ブドウ、グランベリー、ブルーベリー、みかん、オレンジ、レモン、シークワーサーなどを原料としたものは、本発明に係る飲食物に包含される。 Examples of foods and drinks made from vegetables and fruits include vegetable juices, vegetable purees, vegetable soups, green juices, fruit juice drinks, fruit liquors, foods and drinks supplemented with special ingredients contained in vegetables and fruits, etc. But are not limited thereto. Vegetables and fruits as raw materials, for example, tomatoes, carrots, peppers, parsley, celery, spinach, moroheiya, kale, noni, grapes, granberries, blueberries, mandarin oranges, lemons, squeakers, etc. Is included in the food and drink according to the present invention.
穀物・野菜・果実類以外の植物を原料とした飲食物としては、茶葉(緑茶、プーアル茶、どくだみ茶、紅茶など)、コーヒー豆、漢方薬・生薬(イチョウ葉、ウコンなど)、子実体・菌糸体(メシマコブ、アガリスク茸など)、海草(昆布、フコイダンなど)、藻類(クロレラなど)などを用いたものが挙げられるが、それらに限定されない。また、茶葉に含有するカテキン、テアニン、カフェインなど、前記原料に含有する特殊成分を強化した飲食物・サプリメントなども、本発明に係る飲食物に包含される。 Food and drink made from plants other than grains, vegetables, and fruits include tea leaves (green tea, puer tea, dodomi tea, black tea, etc.), coffee beans, herbal medicines and herbal medicines (ginkgo biloba leaves, turmeric, etc.), fruiting bodies, mycelium Examples include, but are not limited to, those using bodies (such as mesimacob and agarisk mochi), seaweed (such as kelp and fucoidan), and algae (such as chlorella). In addition, foods and supplements in which special ingredients contained in the raw materials such as catechin, theanine, and caffeine contained in tea leaves are enhanced are also included in the food and drink according to the present invention.
魚貝類を原料とした飲食物としては、例えば、魚肉ハム、魚肉ソーセージ、蒲鉾、カニ蒲鉾などの練り製品、水煮・味噌煮など加熱調理した缶詰製品、煮る・焼く・蒸す・揚げるなど加工調理した惣菜品・レトルト食品・冷凍食品、DHA・EPAなど魚貝類に含有する特殊成分を強化した飲食物・サプリメント、などが挙げられるが、それらに限定されない。例えば、マグロ、サバ、イワシ、スケトウダラ、サメ、カニ、エビ、イカ、タコ、アサリ、ハマグリ、サザエ、赤貝などを原料としたものは、本発明に係る飲食物に包含される。 As food and drink made from fish and shellfish, for example, fish ham, fish sausage, koji, crab rice and other kneaded products, cooked canned products such as boiled and miso boiled, boiled, bake, steamed and fried Examples include, but are not limited to, prepared foods, retort foods, frozen foods, foods / supplements supplemented with special ingredients contained in fish and shellfish such as DHA / EPA, and the like. For example, foods using tuna, mackerel, sardines, walleye pollock, shark, crab, shrimp, squid, octopus, clams, clams, turban shells, red shellfish and the like are included in the food and drink according to the present invention.
畜産物を原料とした飲食物としては、例えば、ベーコン、ハム、ソーセージ、ハンバーグ、サラミ、ゼリー、ゼラチンゼリーなどをはじめとする畜肉加工食品類、乳飲料、ホイップクリーム、アイスクリームなどをはじめとする乳加工飲食物、プリン、カスタードクリーム、玉子焼き、茶碗蒸しなどをはじめとする卵加工食品類、などが挙げられるが、それらに限定されない。なお、原料となる畜産物の種類は、特に限定されない。 Examples of foods and drinks made from livestock products include processed meat products such as bacon, ham, sausage, hamburger, salami, jelly, gelatin jelly, milk beverages, whipped cream, ice cream, etc. Examples include, but are not limited to, processed milk foods, pudding, custard cream, egg-baked eggs, egg-processed foods such as steamed rice cakes, and the like. In addition, the kind of livestock product used as a raw material is not specifically limited.
微生物を原料とした飲食物としては、例えば、味噌、醤油、タレ、酵母エキスなどの調味料、ヨーグルトやヨーグルト飲料、納豆などが挙げられるが、それらに限定されない。酵母、カビ、乳酸菌、納豆菌などの発酵を利用した飲食物は、本発明に係る飲食物に包含される。 Examples of foods and drinks made from microorganisms include, but are not limited to, seasonings such as miso, soy sauce, sauce, and yeast extract, yogurt, yogurt drink, and natto. Foods and drinks utilizing fermentation of yeast, mold, lactic acid bacteria, natto bacteria, etc. are included in the foods and drinks according to the present invention.
加熱殺菌などの製造工程の際に副生した不快味・不快臭を有する飲食物としては、例えば、カレー、ハヤシ、おでん・米飯類などの調理済み食品、コンソメスープ、ポタージュなどのレトルト食品・缶詰食品などが挙げられるが、それらに限定されない。 Examples of foods and drinks that have an unpleasant taste and unpleasant odor by-produced during the manufacturing process such as heat sterilization include cooked foods such as curry, hayashi, oden and cooked rice, retort foods such as consomme soup and potage, and canned food Although foodstuff etc. are mentioned, it is not limited to them.
その他、例えば、アミノ酸類、ペプチド類、タンパク類、ビタミン類、ミネラル類(Ca、Na、K、Znなど)、高甘味度甘味料類、有機酸類、脂肪酸類、及び、これらを用いた飲食物・サプリメントなども、本発明に係る飲食物に包含される。 In addition, for example, amino acids, peptides, proteins, vitamins, minerals (Ca, Na, K, Zn, etc.), high-intensity sweeteners, organic acids, fatty acids, and food and drink using these -Supplements etc. are also included in the food and drink according to the present invention.
加えて、本発明に係る糖又は糖組成物は、経口摂取可能な医薬品・化粧品などに適用した場合にも、不快味・不快臭を低減でき、それ以外の医薬品・化粧品などに適用した場合についても、不快臭を低減できる。また、本発明に係る糖又は糖組成物は、容器・繊維などの化成品に適用した場合についても、不快臭を低減できるため、例えば、前記飲食物を収容した容器の持つ不快臭(容器臭)も有効に低減できる。 In addition, the sugar or sugar composition according to the present invention can reduce unpleasant taste and unpleasant odor even when applied to orally ingestible pharmaceuticals and cosmetics, and is applied to other pharmaceuticals and cosmetics. Can also reduce unpleasant odor. In addition, the sugar or sugar composition according to the present invention can reduce unpleasant odor even when applied to chemical products such as containers and fibers. ) Can also be effectively reduced.
なお、本発明に係る不快味・不快臭低減作用が阻害・抑制されない限り、例えば、他の糖組成物や防腐剤、安定剤といった他の食品添加物を含んでいても良い。 In addition, as long as the unpleasant taste / unpleasant odor reducing action according to the present invention is not inhibited or suppressed, other food additives such as other sugar compositions, preservatives, and stabilizers may be included.
本発明に係るグルコースを構成糖とした三糖類又はその還元物により、不快味・不快臭を低減することができる。 An unpleasant taste and unpleasant odor can be reduced by the trisaccharide having glucose as a constituent sugar or a reduced product thereof according to the present invention.
実施例1は、パノースの、不快味・不快臭低減効果を示したものである。なお、実施例におけるパノース濃度は、固形分換算での濃度を示す。 Example 1 shows the unpleasant taste / unpleasant odor reduction effect of panose. In addition, the panose density | concentration in an Example shows the density | concentration in conversion of solid content.
市販の有機豆乳(名古屋製酪株式会社製、大豆固形分10重量%、以下同じ)に、市販のパノース(生化学工業株式会社製:パノース97重量%以上含有、以下同じ)を、パノースの最終濃度(重量%)が表1左欄に示す値になるように添加し、4℃で1日保存した。そして、これらの豆乳調製品について、7人による官能評価を行った。 The final panose of commercially available organic soymilk (Nagoya Dairy Co., Ltd., soybean solid content 10% by weight, the same applies hereinafter) and commercially available panose (Seikagaku Corporation: containing 97% by weight or more of panose, the same applies hereinafter) It added so that a density | concentration (weight%) might become the value shown in the left column of Table 1, and it preserve | saved at 4 degreeC for 1 day. And about these soymilk preparations, sensory evaluation by seven people was performed.
官能評価は、不快味又は不快臭の改善効果が強いものを「◎」、改善効果があるものを「○」、改善効果が弱いものを「△」、改善効果のないものを「×」として、判定した(以下同じ)。また、ポータブルオドメーターFPO−1(双葉エレクトロニクス社製、以下同じ)を用いて、臭い強度を測定し、その結果を、無添加の臭い強度を100とした時の相対値で示した。結果を表1に示す。 For sensory evaluation, “◎” indicates that the effect of improving the unpleasant taste or odor is strong, “○” indicates that the improvement is effective, “△” indicates that the improvement is weak, and “×” indicates that there is no improvement. (The same applies hereinafter). Moreover, the odor intensity | strength was measured using portable odometer FPO-1 (Futaba Electronics Co., Ltd. make, the same hereafter), and the result was shown by the relative value when an additive-free odor intensity | strength is set to 100. FIG. The results are shown in Table 1.
表1に示すように、パノース含量が、最終濃度0.2重量%以上で、ある程度の不快味・不快臭低減作用が見られ、最終濃度2.0重量%になると、明らかな不快味・不快臭低減作用があった。従って、少なくとも、パノースの最終濃度が0.2重量%以上になるように、パノースを含有する飲食物原料などの糖組成物を飲食物などに添加することにより、不快味・不快臭低減作用を得ることができると考えられる。 As shown in Table 1, when the final concentration is 0.2% by weight or more, a certain amount of unpleasant taste / unpleasant odor reduction effect is seen, and when the final concentration is 2.0% by weight, the obvious unpleasant taste / unpleasant There was an odor reducing effect. Therefore, by adding a sugar composition such as a raw material for food and drink containing panose to food and drink so that the final concentration of panose is at least 0.2% by weight, the effect of reducing unpleasant taste and unpleasant odor is obtained. It is thought that it can be obtained.
実施例2は、パノース含有糖組成物の不快味・不快臭低減効果を示した実験である。 Example 2 is an experiment showing the effect of reducing the unpleasant taste and unpleasant odor of the panose-containing sugar composition.
市販の有機豆乳に、それぞれ、パノース20重量%含有糖組成物、パノース15重量%含有糖組成物、及び、パノース10重量%含有糖組成物を、パノースの最終含有量が0.5重量%となるようにそれぞれ調製し、4℃で1日保存した。また、対照として、パノース無添加品(パノースの最終濃度0重量%)と、市販のパノースを最終濃度0.5重量%になるように添加したものについても、同様の処理を行った。そして、これらの豆乳調製品について、官能評価を行った。結果を表2に示す。 In a commercially available organic soymilk, a sugar composition containing 20% by weight of panose, a sugar composition containing 15% by weight of panose, and a sugar composition containing 10% by weight of panose, respectively, with a final panose content of 0.5% by weight. Each was prepared and stored at 4 ° C. for 1 day. In addition, as a control, the same treatment was performed on a panose-free product (final concentration of panose 0% by weight) and a commercial panose added to a final concentration of 0.5% by weight. And sensory evaluation was performed about these soymilk preparations. The results are shown in Table 2.
なお、パノース含有糖組成物は、デンプン液化液からマルトース生成アミラーゼを添加して調製できるマルトース液にα−グルコシダーゼを作用させて、パノース含有量が固形分換算で、それぞれ、10、15、20%となるように転移反応を進めることにより製造し、精製後、固形分75%まで濃縮して用いた。 It should be noted that the panose-containing sugar composition is prepared by allowing α-glucosidase to act on a maltose liquid that can be prepared by adding maltose-producing amylase from a starch liquefaction liquid, and the panose content is 10, 15, and 20% in terms of solid content, respectively. The product was produced by advancing the transfer reaction so as to be, and after purification, it was concentrated to a solid content of 75% and used.
官能評価は、不快味低減効果、不快臭低減効果、豆乳のおいしさ、総合評価について、判定を行った。不快味低減効果及び不快臭低減効果については、実施例1と同様の基準で評価した。豆乳の「おいしさ」の評価は、豆乳そのものの味を全く損なわないものを「◎」、豆乳そのものの味をほとんど損なわないものを「○」、豆乳そのものの味をやや損ねているものを「△」、豆乳そのものの味を損ねているものを「×」として、判定した(以下同じ)。また、「総合評価」は、不快味又は不快臭の改善効果が強く、かつ、飲食物のおいしさを全く損ねていないものを「◎」、不快味又は不快臭の改善効果があり、かつ、飲食物のおいしさを損ねていないものを「○」、飲食物のおいしさがやや損なわれているものを「△」、飲食物のおいしさが損なわれているものを「×」として、判定した(以下同じ)。 Sensory evaluation evaluated about the unpleasant taste reduction effect, the unpleasant smell reduction effect, the deliciousness of soymilk, and comprehensive evaluation. The unpleasant taste reducing effect and the unpleasant odor reducing effect were evaluated according to the same criteria as in Example 1. The evaluation of the “taste” of soymilk is based on “◎” if the taste of the soymilk itself is not impaired at all, “○” if it does not substantially impair the taste of the soymilk itself, “△” and those that had a bad taste of the soymilk itself were determined as “x” (the same applies hereinafter). In addition, “Comprehensive evaluation” is “◎” when the effect of improving unpleasant taste or unpleasant odor is strong and does not impair the taste of food or drink, and has the effect of improving unpleasant taste or unpleasant odor, and Judge that the taste of food and drink is not impaired as “○”, that the taste of food and drink is slightly impaired as “△”, and that the taste of food and drink is impaired as “x” (The same applies hereinafter).
表2に示すとおり、パノースを最終濃度0.5重量%含有している豆乳調製品は、無添加品と比較して、不快味・不快臭が低減された。パノースを含有している豆乳調製品については、パノース15%及び10%含有糖組成物を用いた場合、パノース20%含有糖組成物を用いた場合と比較して、おいしさが損なわれていた。 As shown in Table 2, the soymilk preparation containing panose at a final concentration of 0.5% by weight had reduced unpleasant taste and unpleasant odor as compared to the additive-free product. About the soymilk preparation containing panose, when panose 15% and 10% containing sugar composition was used, compared with the case where panose 20% containing sugar composition was used, the deliciousness was impaired. .
これは、パノース15重量%含有糖組成物、パノース10重量%含有糖組成物の場合、パノースの最終濃度を0.5重量%にするためにパノース含有糖組成物を比較的多量に添加する必要があることから、これらの糖組成物の甘味などが強すぎ、全体の味、飲み易さを損ねたためと考えられる。 In the case of a sugar composition containing 15% by weight of panose and a sugar composition containing 10% by weight of panose, it is necessary to add a relatively large amount of the panose-containing sugar composition in order to make the final concentration of panose 0.5% by weight. Therefore, it is considered that the sweetness of these sugar compositions was too strong, and the overall taste and ease of drinking were impaired.
従って、飲食物の味を損なわずに、不快味・不快臭低減作用効果を得るためには、少なくともパノース20重量%以上含有糖組成物を用いるのが最も効果的であることが、本実験により示された。 Therefore, according to this experiment, it is most effective to use a sugar composition containing at least 20% by weight of panose in order to obtain an effect of reducing unpleasant taste and unpleasant odor without impairing the taste of food and drink. Indicated.
実施例3では、豆乳調製物に含まれる1−ヘキサナールの定量と不快臭低減に関する官能評価を行った。1−ヘキサナールは、豆乳の主な臭い成分である。実験手順は以下のとおりである。 In Example 3, the sensory evaluation regarding the fixed_quantity | quantitative_assay of 1-hexanal contained in a soymilk preparation and unpleasant odor reduction was performed. 1-hexanal is the main odor component of soy milk. The experimental procedure is as follows.
市販の有機豆乳に、それぞれ、パノース20重量%含有糖組成物、トレハロース(生化学工業株式会社製:トレハロース純度99%以上)、砂糖、を各5重量%(固形分換算)加えて、4℃で1日保存した。これらの豆乳調製品について、1−ヘキサナールの定量を行い、また、官能評価を行った。1−ヘキサナールの定量は、固相マイクロ抽出(SPME)法による前処理後、ガスクロマトグラフ質量分析装置を用いて行った。 To each commercially available organic soymilk, 5% by weight of each panose 20% by weight sugar composition, trehalose (manufactured by Seikagaku Corporation: trehalose purity 99% or more) and sugar are added (in terms of solid content) at 4 ° C. And stored for 1 day. About these soymilk preparations, 1-hexanal was quantified and sensory evaluation was performed. 1-hexanal was quantified using a gas chromatograph mass spectrometer after pretreatment by a solid phase microextraction (SPME) method.
固相マイクロ抽出(SPME)法は、次の手順で行った。まず、20mlバイアル(SUPELCO社製)に、10mlの前記豆乳調製品を入れ、さらに、20ppmのシクロヘキサノン(Cyclohexanone、内部標準物質)を添加した。次に、前記バイアルを、テフロン(登録商標)シールされたセプタムキャップ(SUPELCO社製)で密栓し、60℃、30分間、予備加熱した。次に、前記バイアルのヘッドスペース部分にSPMEファイバーを30分間投入し、揮発成分の抽出を行った。SPMEファイバーには、肥厚75μmのCarboxen/PDMS(ポリジメチルシロキサン)ファイバー(SUPELCO社製)を用いた。なお、SPMEファイバーは、300℃、1時間、クリーニングを行ったものを用いた。 The solid phase microextraction (SPME) method was performed according to the following procedure. First, 10 ml of the soymilk preparation was placed in a 20 ml vial (supplico), and 20 ppm of cyclohexanone (Cyclohexanone, internal standard substance) was added. Next, the vial was sealed with a Teflon (registered trademark) sealed septum cap (manufactured by SUPELCO) and preheated at 60 ° C. for 30 minutes. Next, SPME fiber was put into the head space portion of the vial for 30 minutes to extract volatile components. As the SPME fiber, a Carboxen / PDMS (polydimethylsiloxane) fiber (SUPELCO) having a thickness of 75 μm was used. In addition, the SPME fiber used what cleaned 300 degreeC and 1 hour.
次に、ガスクロマトグラフ質量分析装置のサンプル注入口に、SPMEファイバー(抽出成分を含有)を入れ、内部標準物質のピーク面積・ピーク高さに基づき、各豆乳調製品に含有する1−ヘキサナールを定量した。ガスクロマトグラフ質量分析装置はGCMS−QP5050A(株式会社島津製作所製)を用いた。キャピラリーカラムはTC−FFAP(ジーエルサイエンス株式会社製)を用いた。キャリアガスはヘリウムガスを用いて、流量は1ml/minに設定した。サンプル注入口の温度は250℃とし、カラムの温度は40℃、2分間の後、5℃/minで200℃まで昇温させ、200℃で4分間維持した。キャピラリーカラムへの抽出成分の導入は、スプリットレス方式により行った。質量分析は、30〜400m/zの範囲で行った。なお、キャピラリーカラムは、各測定前に、240℃、5分間、クリーニングを行った。 Next, SPME fiber (containing extracted components) is put into the sample inlet of the gas chromatograph mass spectrometer, and 1-hexanal contained in each soymilk preparation is quantified based on the peak area and peak height of the internal standard substance. did. As the gas chromatograph mass spectrometer, GCMS-QP5050A (manufactured by Shimadzu Corporation) was used. As the capillary column, TC-FFAP (manufactured by GL Sciences Inc.) was used. The carrier gas was helium gas and the flow rate was set to 1 ml / min. The temperature of the sample inlet was 250 ° C., the temperature of the column was 40 ° C., 2 minutes later, the temperature was raised to 200 ° C. at 5 ° C./min, and maintained at 200 ° C. for 4 minutes. The extraction component was introduced into the capillary column by the splitless method. Mass spectrometry was performed in the range of 30 to 400 m / z. The capillary column was cleaned at 240 ° C. for 5 minutes before each measurement.
官能評価は、「◎」豆臭を顕著に抑制したもの、「○」豆臭をある程度抑制したもの、「△」豆臭を僅かに抑制したもの、「×」豆臭を全く抑制しなかったもの、とした。 The sensory evaluation was that “◎” bean odor was significantly suppressed, “○” bean odor was suppressed to some extent, “△” bean odor was slightly suppressed, and “×” bean odor was not suppressed at all. Stuff.
各豆乳調製物における1−ヘキサナールの定量結果、及び、官能評価を表3に示す。
表3に示すとおり、1−ヘキサナール(豆乳の主な臭い成分)の検出量は、トレハロースとパノース20重量%含有糖組成物で減少しており、特に、パノース20重量%含有糖組成物ではトレハロースよりも顕著に減少していた。また、1−ヘキサナールの定量結果は、官能評価とも一致していた。従って、本実験結果は、パノース20重量%含有糖組成物が、不快臭低減作用を有し、飲食物の風味などの保持改善に有効であることを示している。 As shown in Table 3, the detected amount of 1-hexanal (the main odor component of soy milk) is decreased in the sugar composition containing trehalose and panose 20% by weight, and particularly in the sugar composition containing panose 20% by weight, trehalose. Was significantly less than. Moreover, the quantitative result of 1-hexanal was consistent with the sensory evaluation. Therefore, this experimental result shows that the sugar composition containing 20% by weight of panose has an effect of reducing unpleasant odor and is effective in improving the maintenance of the flavor and the like of food and drink.
実施例4は、パノース、トレハロースの、不快味・不快臭低減効果を比較したものである。 Example 4 compares panose and trehalose in reducing the unpleasant taste and unpleasant odor.
市販の有機豆乳に、それぞれ、パノース20重量%含有糖組成物、トレハロース(生化学工業株式会社製:トレハロース純度99%以上)を各10重量%(固形分換算)加えて、4℃で1日保存した。これらの豆乳調製品について、実施例2と同様に官能評価を行い、また、実施例1と同様に臭い強度を測定した。結果を表4に示す。 Panos 20% by weight containing sugar composition and trehalose (manufactured by Seikagaku Corporation: Trehalose purity 99% or more) each 10% by weight (in terms of solid content) were added to commercially available organic soymilk for 1 day at 4 ° C. saved. These soymilk preparations were subjected to sensory evaluation in the same manner as in Example 2, and the odor intensity was measured in the same manner as in Example 1. The results are shown in Table 4.
その結果、パノース20重量%含有糖組成物(パノースの最終濃度は2.0重量%)では、トレハロースよりも明らかに不快味・不快臭低減効果を得ることができた。また、パノース20重量%含有糖組成物のほうが、トレハロースと比べて、おいしさを損なわず、良好な風味を保持できることがわかった。このことは、パノース20重量%含有糖組成物が、トレハロースよりも、有効な不快味・不快臭低減作用を有し、飲食物の風味などの保持改善に有効であることを示している。 As a result, in the sugar composition containing 20% by weight of panose (final concentration of panose is 2.0% by weight), an effect of reducing unpleasant taste and unpleasant odor was clearly obtained compared with trehalose. In addition, it was found that the sugar composition containing 20% by weight of panose can maintain a good flavor without impairing the deliciousness as compared with trehalose. This indicates that the sugar composition containing 20% by weight of panose has an effect of reducing the unpleasant taste and unpleasant odor more effectively than trehalose, and is effective in improving the retention of the flavor of food and drink.
また、この実験結果は、パノース20重量%含有糖組成物が、豆乳のもつ不快味・不快臭を有効に低減することができることを示している。豆乳は、甘味成分などを添加して、味を調整する場合が多いので、本発明に係るパノース20重量%含有糖組成物を添加すれば、豆乳飲料のおいしさを損なうことなく、程よいコク味・旨味・甘味を添加し、さらに、不快味・不快臭を低減することができる。 Moreover, this experimental result has shown that the sugar composition containing 20 weight% of panose can reduce the unpleasant taste and unpleasant odor which soymilk has effectively. Soy milk often has a sweet taste component or the like to adjust the taste. Therefore, if the sugar composition containing 20% by weight of panose according to the present invention is added, a moderately rich taste can be obtained without impairing the deliciousness of the soy milk beverage. -Add umami and sweetness, and reduce unpleasant taste and unpleasant odor.
実施例5は、にんじんジュースに対する不快味・不快臭低減効果を示した実験である。 Example 5 is an experiment showing an unpleasant taste / unpleasant odor reduction effect on carrot juice.
にんじん500gをジューサーに入れ粉砕した後、ガーゼを用いて濾過し、にんじん溶液を調製した。得られたにんじん溶液に、それぞれ、パノース20重量%含有糖組成物、トレハロース(商品名トレハオース、林原生化学研究所株式会社製、以下同じ)、マルトース(商品名ファイントース、林原生化学研究所株式会社製、以下同じ)、及び砂糖(対照)を、それぞれ10重量%(固形分換算)になるように添加し、4℃で1日保存した。上記の調製品について実施例1と同様の評価基準により官能評価を行った。その結果を表5に示す。 500 g of carrot was put in a juicer and ground, and then filtered using gauze to prepare a carrot solution. Each of the resulting carrot solutions contains 20% by weight of panose sugar composition, trehalose (trade name Trehaose, Hayashibara Biochemical Laboratories Co., Ltd., the same shall apply hereinafter), maltose (trade name Finetose, Hayashibara Biochemistry Laboratories) Company (same as below) and sugar (control) were added to 10% by weight (in terms of solid content) and stored at 4 ° C. for 1 day. Sensory evaluation was performed according to the same evaluation criteria as Example 1 about said preparation. The results are shown in Table 5.
表5に示すとおり、マルトースでは不快味・不快臭低減効果はほとんど見られなかった。トレハロースは、ある程度の不快味・不快臭低減改善効果は得られたが、トレハロース添加により、にんじんジュースにはない別の味が引き立ち、おいしさが損なわれていた。一方、パノース20重量%含有糖組成物は、他の調製品と比較して、明らかに、不快味・不快臭低減効果が得られた。 As shown in Table 5, maltose hardly showed an unpleasant taste / unpleasant odor reducing effect. Although trehalose was able to obtain some unpleasant taste and unpleasant odor reduction improvement effect, the addition of trehalose emphasized another taste not found in carrot juice, and the taste was impaired. On the other hand, the sugar composition containing 20% by weight of panose clearly obtained an unpleasant taste / unpleasant odor reduction effect as compared with other preparations.
にんじんジュースなど野菜を添加したジュースは、甘味成分などを添加して、味を調整する場合が多い。本実験により、本発明に係るパノース20重量%含有糖組成物を添加すれば、野菜ジュースなどのおいしさを損なうことなく、程よいコク味・旨味・甘味などを添加して飲み易くし、さらに、不快味・不快臭を低減することができることが示された。また、パノースは、野菜ジュースなどのおいしさを損なうことなく、不快味・不快臭を低減することができる点で、トレハロースよりも有利性がある。 In many cases, juice such as carrot juice is added to adjust the taste by adding sweetening ingredients. By adding the sugar composition containing 20% by weight of panose according to the present invention, it is easy to drink by adding moderate richness, umami, sweetness, etc. without impairing the taste of vegetable juice, It was shown that unpleasant taste and unpleasant odor can be reduced. In addition, panose has an advantage over trehalose in that it can reduce unpleasant taste and unpleasant odor without impairing the taste of vegetable juice and the like.
実施例6は、青汁に対する不快味・不快臭低減効果(エグ味・青臭さの抑制)を確認した実験である。 Example 6 is an experiment in which an unpleasant taste / unpleasant odor reduction effect (inhibition of egy taste / blue odor) on green juice was confirmed.
市販の大麦若葉スーパー100(オリヒロ株式会社製)を用いて、10重量%大麦青葉溶液を調製した。得られた大麦青葉溶液に、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖(対照)を、10重量%(固形分換算)になるように添加し、4℃で1日保存した。上記の調製品について実施例1と同様の評価基準により官能評価を行った。その結果を表6に示す。 A 10 wt% barley green leaf solution was prepared using a commercially available barley young leaf super 100 (manufactured by Orihiro Co., Ltd.). To the obtained barley green leaf solution, 20% by weight of panose containing sugar composition, trehalose, maltose, and sugar (control) were added so as to be 10% by weight (in terms of solid content), respectively, at 4 ° C. for 1 day. saved. Sensory evaluation was performed according to the same evaluation criteria as Example 1 about said preparation. The results are shown in Table 6.
表6に示すとおり、マルトースでは不快味・不快臭低減効果はほとんど見られなかった。トレハロースでは、ある程度の不快味・不快臭低減効果が見られた。それに対し、パノース20重量%含有糖組成物は、トレハロース、マルトースと比較して、顕著に、エグ味・青臭さを低減する効果が得られた。 As shown in Table 6, maltose hardly showed an unpleasant taste / unpleasant odor reducing effect. Trehalose had some unpleasant taste and unpleasant odor reduction effect. On the other hand, the sugar composition containing 20% by weight of panose significantly reduced the taste and blue odor compared to trehalose and maltose.
青汁は、一般に苦いため、甘味成分などを添加して、味を調整する場合が多い。また、青汁独特のエグ味、青臭さがあるため、不快味・不快臭を低減して、飲み易くすることが求められる。本実験により、本発明に係るパノース20重量%含有糖組成物は、青汁の不快味・不快臭を低減して、飲みやすくする効果が顕著であることが示された。 Since green juice is generally bitter, it often has a sweet taste component to adjust its taste. In addition, since there is an egg taste and a blue odor peculiar to green juice, it is required to reduce the unpleasant taste and unpleasant odor and make it easy to drink. From this experiment, it was shown that the sugar composition containing 20% by weight of panose according to the present invention has a remarkable effect of reducing the unpleasant taste and unpleasant odor of green juice and making it easy to drink.
実施例7は、アルコール飲料に対する不快味・不快臭低減効果(苦味・アルコール臭の抑制)を確認した実験である。 Example 7 is an experiment in which an unpleasant taste / unpleasant odor reduction effect (inhibition of bitterness / alcohol smell) with respect to an alcoholic beverage was confirmed.
市販のアルコール飲料として、森羅(合同酒精株式会社製、アルコール分20重量%)を用いて、5重量%アルコール溶液を調製した。得られた5重量%アルコール溶液に、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖(対照)を、それぞれ10重量%(固形分換算)になるように添加し、4℃で1日保存した。上記の調製品について実施例1と同様の評価基準により官能評価を行った。その結果を表7に示す。 As a commercially available alcoholic beverage, 5% by weight alcohol solution was prepared using Mori (God Shusei Co., Ltd., alcohol content 20% by weight). To the obtained 5% by weight alcohol solution, 20% by weight panose-containing sugar composition, trehalose, maltose, and sugar (control) were respectively added so as to be 10% by weight (in terms of solid content). And stored for 1 day. Sensory evaluation was performed according to the same evaluation criteria as Example 1 about said preparation. The results are shown in Table 7.
表7に示すとおり、マルトースでは、不快味・不快臭低減効果はほとんど見られなかった。トレハロースでは、ある程度の不快味・不快臭低減効果が見られた。それに対し、パノース20重量%含有糖組成物は、トレハロースと比較して、顕著な不快味・不快臭低減効果が得られた。 As shown in Table 7, maltose hardly showed an unpleasant taste / unpleasant odor reducing effect. Trehalose had some unpleasant taste and unpleasant odor reduction effect. On the other hand, the sugar composition containing 20% by weight of panose has a remarkable effect of reducing unpleasant taste and unpleasant odor as compared with trehalose.
アルコール飲料は、独特の苦味、鼻をさすアルコール臭があるため、それらの微細な不快味・不快臭を低減することが望ましい。本実験により、本発明に係るパノース20重量%含有糖組成物は、アルコール飲料特有の苦味、アルコール臭を低減する効果があることから、アルコール飲料自体も飲み易いものにすることができるという効果があることが示された。 Alcoholic beverages have a unique bitter taste and a nose-like alcohol odor. Therefore, it is desirable to reduce such fine unpleasant taste and unpleasant odor. According to this experiment, the sugar composition containing 20% by weight of panose according to the present invention has the effect of reducing the bitterness and alcohol odor peculiar to alcoholic beverages, so that the alcoholic beverage itself can be easily drinkable. It was shown that there is.
実施例8は、黒酢飲料に対する不快味・不快臭低減効果(酸味・酸臭の抑制)を確認した実験である。 Example 8 is an experiment in which an unpleasant taste / unpleasant odor reduction effect (suppression of acidity / acid odor) for black vinegar beverages was confirmed.
市販の黒酢(キューピー醸造株式会社:酸度3.5)を用いて、黒酢酸度が0.4重量%になるように、黒酢溶液を調製した。得られた黒酢溶液に、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖(対照)をそれぞれ10重量%(固形分換算)になるように添加し、黒酢飲料を調製後、4℃で1日保存した。上記の調製品について実施例1と同様の評価基準により官能評価を行った。その結果を表8に示す。 A black vinegar solution was prepared using a commercially available black vinegar (Cuppy Brewing Co., Ltd .: acidity 3.5) so that the degree of black acetate was 0.4% by weight. To each of the obtained black vinegar solutions, 20% by weight panose-containing sugar composition, trehalose, maltose, and sugar (control) are each added to 10% by weight (in terms of solid content) to prepare a black vinegar beverage. Thereafter, it was stored at 4 ° C. for 1 day. Sensory evaluation was performed according to the same evaluation criteria as Example 1 about said preparation. The results are shown in Table 8.
表8に示すとおり、マルトースは、不快味・不快臭低減効果をほとんど確認できなかった。トレハロースは、酸味・酸臭を和らげる効果はある程度見られた。それに対し、パノース20重量%含有糖組成物は、トレハロース、マルトースと比較して、顕著な不快味・不快臭低減効果が得られた。 As shown in Table 8, maltose could hardly confirm an unpleasant taste / unpleasant odor reducing effect. Trehalose was found to have some effect of reducing acidity and acid odor. On the other hand, the sugar composition containing 20% by weight of panose has a remarkable effect of reducing unpleasant taste and unpleasant odor as compared with trehalose and maltose.
黒酢は、健康食品として、直接又は他の飲料と混ぜて飲んだり、料理に用いたりなどするが、酸味、酸臭が強いため、それらの不快味・不快臭を低減することが望ましい。本実験により、本発明に係るパノース20重量%含有糖組成物は、黒酢特有の酸味、酸臭を低減する効果があることから、黒酢を飲み易いものにすることができるという効果があることが示された。また、黒酢を料理に使用する場合でも、料理に加える砂糖、みりんなどのコク味・旨味・甘味などの代わりに、本発明に係るパノース含有糖組成物を用いることもでき、その料理のおいしさを損なうことなく、同時に、不快味・不快臭を低減することができると考える。 Black vinegar is used as a health food, either directly or mixed with other beverages, or used for cooking. However, since it has a strong acidity and acid odor, it is desirable to reduce those unpleasant tastes and unpleasant odors. From this experiment, the sugar composition containing 20% by weight of panose according to the present invention has the effect of reducing the sourness and acid odor peculiar to black vinegar, so that it is possible to make black vinegar easy to drink. It was shown that. In addition, even when black vinegar is used for cooking, the panose-containing sugar composition according to the present invention can be used in place of sugar, mirin, and other sweetness, umami, and sweetness added to the dish. It is considered that unpleasant taste and unpleasant odor can be reduced at the same time without impairing the thickness.
実施例9は、レトルト食品に対する不快臭改善効果(加熱殺菌臭及び容器臭の抑制)を確認した実験である。 Example 9 is an experiment in which the effect of improving unpleasant odor against retort food (suppression of heat sterilization odor and container odor) was confirmed.
コンソメスープに、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖(対照)を、それぞれ10重量%(固形分換算)になるように添加し、オートクレーブ殺菌(120℃、20分)を行った。上記の調製品について実施例2と同様の評価基準により官能評価を行った。その結果を表9に示す。 To the consomme soup, sugar composition containing 20% by weight of panose, trehalose, maltose, and sugar (control) are added so as to be 10% by weight (in terms of solid content), respectively, and autoclaved (120 ° C., 20 minutes) ) Sensory evaluation was performed according to the same evaluation criteria as Example 2 about said preparation. The results are shown in Table 9.
表9に示すとおり、マルトースでは加熱殺菌臭及び容器臭に関する低減効果はほとんど見られなかった。トレハロースは、加熱殺菌臭について、ある程度の不快臭改低減効果が見られたが、トレハロース添加により、コンソメスープの好ましい香りが低減し、おいしさが損なわれていたため、総合評価は、「×」となった。 As shown in Table 9, maltose showed almost no reduction effect on heat sterilization odor and container odor. Trehalose showed a certain degree of unpleasant odor modification effect for heat sterilization odor, but the addition of trehalose reduced the preferred scent of consomme soup and impaired the deliciousness, so the overall evaluation was `` x '' became.
それに対し、パノース20重量%含有糖組成物は、他の調製品と比較して、加熱殺菌臭を有効に低減した。また、レトルトに由来する容器臭も有効に低減した。従って、パノースは、レトルト食品の本来の風味を損なうことなく、加熱殺菌臭及び容器臭を低減できる点で、トレハロースよりも有利性がある。なお、パノースについて、レトルト殺菌についても同様の実験を行った結果、レトルト殺菌の場合でもオートクレーブ殺菌と同様の加熱殺菌臭低減効果が見られた。 In contrast, the sugar composition containing 20% by weight of panose effectively reduced the heat sterilization odor as compared with other preparations. Moreover, the container odor derived from a retort was also reduced effectively. Therefore, panose is more advantageous than trehalose in that it can reduce the heat sterilization odor and container odor without impairing the original flavor of the retort food. In addition, about panose, as a result of performing the same experiment also about retort sterilization, even in the case of retort sterilization, the same heat sterilization odor reduction effect as autoclave sterilization was seen.
レトルト食品には、ほとんどの場合、コク味・旨味・甘味などの成分が添加されているため、それらの成分の代わりに、本願に係るパノース20重量%含有糖組成物を用いれば、レトルト食品のおいしさを損ねることなく、レトルト食品の加熱殺菌臭及びレトルトに由来する容器臭を低減できる。 In most cases, ingredients such as kokumi, umami, and sweet are added to retort foods. Instead of these ingredients, if the sugar composition containing 20% by weight of panose according to the present application is used, Without deteriorating the taste, the heat sterilization odor of the retort food and the container odor derived from the retort can be reduced.
また、本実験により、パノース20重量%含有糖組成物は、加熱によっても、不快臭低減作用を失わないことが示された。従って、本願に係るパノース20重量%含有糖組成物は、製造過程で加熱を要する飲食物に対しても、不快臭低減有効成分として用いることができると考えられる。 Moreover, it was shown by this experiment that the sugar composition containing 20% by weight of panose does not lose the unpleasant odor reducing action even when heated. Therefore, it is considered that the sugar composition containing 20% by weight of panose according to the present application can be used as an effective component for reducing unpleasant odors for foods and drinks that require heating during the production process.
実施例10は、水産加工品であるかまぼこに対する不快味・不快臭低減効果を示した実験である。 Example 10 is an experiment showing an effect of reducing unpleasant taste and unpleasant odor against kamaboko which is a processed fishery product.
試作品のかまぼこは、以下の手順で製造した。冷凍すり身(スケトウダラ)80g、ハモ生すり身20g、食塩3g、糖質、馬鈴薯澱粉15g、卵白5g、グルタミン酸ナトリウム0.5gを、混ぜ合わせた後、蒸し器で蒸し、蒸しかまぼこを製造した。なお、前記糖質として、それぞれ、パノース20重量%含有糖組成物8g、トレハロース8g、マルトース8g、砂糖5gを配合した。砂糖は、砂糖5gと水2gを配合したものを用いた。 The prototype kamaboko was manufactured according to the following procedure. 80 g of frozen surimi (spotted fish), 20 g of ground fish paste, 3 g of salt, carbohydrates, 15 g of potato starch, 5 g of egg white, and 0.5 g of sodium glutamate were mixed together, and then steamed with a steamer to produce a steamed sea bream. As the saccharides, 8 g of panose containing 20% by weight, 8 g of trehalose, 8 g of maltose and 5 g of sugar were blended. As the sugar, a mixture of 5 g of sugar and 2 g of water was used.
上記の試作品について実施例1と同様の評価基準により官能評価を行った。結果を表10に示す。 The above prototype was subjected to sensory evaluation according to the same evaluation criteria as in Example 1. The results are shown in Table 10.
表10に示すとおり、砂糖、マルトースを用いた試作品では、不快味・不快臭低減効果はほとんど見られなかった。パノースとトレハロースを用いた試作品を比較すると、パノースを用いた試作品のほうが、より良好な不快味・不快臭低減効果が見られた。従って、本願に係るパノース20重量%含有糖組成物は、かまぼこなどの魚介類を原料とした食品についても、不快味・不快臭低減作用を有することが示された。 As shown in Table 10, in the prototype using sugar and maltose, the effect of reducing unpleasant taste and unpleasant odor was hardly seen. Comparing the prototypes using panose and trehalose, the prototype using panose showed better unpleasant taste and odor reduction effect. Therefore, it was shown that the sugar composition containing 20% by weight of panose according to the present application has an effect of reducing unpleasant taste and unpleasant odor even for foods made from seafood such as kamaboko.
実施例11は、水産加工品であるナンプラー(魚醤)に対する不快味・不快臭低減効果(魚臭い味・発酵臭・魚臭の抑制)を確認した実験である。 Example 11 is an experiment in which an unpleasant taste / unpleasant odor reduction effect (inhibition of fishy taste / fermentation smell / fish odor) with respect to Nanpura (fish soy), which is a processed fishery product, was confirmed.
市販ナンプラー(魚醤)(商品名:ナンプラー、有紀食品株式会社製)を水で10倍に薄めた調味液のそれぞれに、パノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖(対照)を、それぞれ10重量%(固形分換算)になるように添加した。前記調味液試作品について、実施例2と同様の官能評価を行った。結果を表11に示す。 A sugar composition containing 20% by weight of panose, trehalose, maltose, and sugar (control) in each seasoning solution obtained by diluting commercially available Nampula (fish soy) (trade name: Nampula, Yuki Foods Co., Ltd.) 10 times with water Were added so that each might become 10 weight% (solid content conversion). The sensory evaluation similar to Example 2 was performed about the said seasoning liquid prototype. The results are shown in Table 11.
表11に示すとおり、マルトースでは、不快味・不快臭ともほとんど低減効果は見られなかった。トレハロースは、弱い不快味・不快臭低減効果が見られたが、独特な味に変化し、おいしさが損なわれていた。 As shown in Table 11, maltose showed almost no reduction in both unpleasant taste and unpleasant odor. Trehalose showed a weak unpleasant taste and an unpleasant odor reducing effect, but it changed to a unique taste and the taste was impaired.
一方、パノース20重量%含有糖組成物は、魚臭い味・発酵臭・魚臭などを良好に抑制した。従って、パノースは、ナンプラーのおいしさを損なうことなく、不快味・不快臭を低減することができる点で、トレハロースよりも有利性がある。また、パノース20重量%含有糖組成物は、ナンプラーなどの特有の味や臭いを有する食品についても、不快味・不快臭低減作用を有することが示された。 On the other hand, the sugar composition containing 20% by weight of panose satisfactorily suppressed fishy taste, fermentation odor, fishy odor and the like. Therefore, panose is more advantageous than trehalose in that it can reduce unpleasant taste and unpleasant odor without impairing the deliciousness of the Nampula. Moreover, it was shown that the sugar composition containing 20% by weight of panose has an unpleasant taste / unpleasant odor reducing action even for foods having a peculiar taste and odor such as Nampula.
実施例12は、大豆サポニンに対する不快味・不快臭低減効果(エグ味・渋味の抑制)を確認した実験である。 Example 12 is an experiment in which the effect of reducing the unpleasant taste and unpleasant odor against soybean saponin (suppression of egy taste and astringency) was confirmed.
市販の大豆サポニン(和光純薬工業株式会社製)0.5重量%溶液のそれぞれに、パノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖(対照)を、それぞれ10%(固形分換算)になるように添加して、不快味(エグ味)・不快臭(渋味)の評価を行った。その結果、表12に示す。 Commercially available soybean saponin (manufactured by Wako Pure Chemical Industries, Ltd.) 0.5% by weight solution each containing 10% panose 20% sugar composition, trehalose, maltose, and sugar (control) (in terms of solid content) ) And the unpleasant taste (egg taste) and unpleasant odor (astringency) were evaluated. The results are shown in Table 12.
表12に示したとおり、マルトース、トレハロースでは、ある程度の不快味・不快臭低減効果しか見られなかったのに対し、パノース20重量%含有糖組成物は、サポニンに由来するエグ味・渋味を有効に軽減することがわかった。 As shown in Table 12, maltose and trehalose showed only a certain amount of unpleasant taste / unpleasant odor reduction effect, whereas the sugar composition containing 20% by weight of panose has the taste and astringency derived from saponin. It was found that it was effectively reduced.
大豆サポニンは、大豆に含まれる栄養成分で、脂肪の蓄積を防止する作用、血液中のコレステロールを低下する作用、抗菌作用などを有する。サポニンを含有する飲食物に、本発明に係る糖組成物を添加することにより、サポニン由来のエグ味・渋味を低減し、サポニンを含有する飲食物の風味を改善することができる。 Soybean saponin is a nutrient contained in soybeans and has an action of preventing fat accumulation, an action of lowering cholesterol in blood, an antibacterial action and the like. By adding the sugar composition according to the present invention to a food or drink containing saponin, the taste and astringency derived from saponin can be reduced, and the taste of food or drink containing saponin can be improved.
実施例13は、茶飲料に対する不快味低減効果(苦味・渋味の抑制)を確認した実験である。 Example 13 is an experiment in which an unpleasant taste reduction effect (inhibition of bitterness and astringency) for a tea beverage was confirmed.
600mg/lのカテキンを含む市販の茶飲料(商品名カテキン緑茶、サンガリア株式会社製)に、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖(対照)を、それぞれ10重量%(固形分換算)になるように添加した。そして、上記の調製品について実施例1と同様の評価基準により官能評価を行った。その結果を表13に示す。 Commercially available tea beverages containing 600 mg / l catechin (trade name catechin green tea, manufactured by Sangalia Co., Ltd.), panose 20% by weight-containing sugar composition, trehalose, maltose and sugar (control), respectively, 10% by weight It added so that it might become (solid content conversion). And sensory evaluation was performed about the said preparation by the evaluation criteria similar to Example 1. FIG. The results are shown in Table 13.
表13に示すとおり、マルトースには、不快味低減効果は確認できなかった。トレハロースは、かなり良好な不快味低減効果が見られたが、トレハロース添加により、独特の味に変化した。それに対し、パノース20重量%含有糖組成物は、トレハロースよりもより良好な不快味低減効果が見られた。 As shown in Table 13, maltose was not confirmed to have an unpleasant taste reducing effect. Although trehalose had a considerably good unpleasant taste reducing effect, it changed to a unique taste by addition of trehalose. On the other hand, the sugar composition containing 20% by weight of panose showed a better unpleasant taste reducing effect than trehalose.
パノースは、茶飲料本来の風味を損なうことなく、苦味・渋味などの不快味を低減することができる点で、トレハロースよりも有利性がある。また、上記の結果より、パノース20重量%含有糖組成物は、茶飲料以外の、苦味・渋味を有する健康食品などに対しても、適用可能であると考えられる。 Panose has an advantage over trehalose in that it can reduce unpleasant tastes such as bitterness and astringency without impairing the original flavor of tea beverages. From the above results, it is considered that the panose 20% by weight-containing sugar composition is applicable to health foods having a bitter taste and astringency other than tea beverages.
実施例14は、小豆こしあんの不快味低減効果(エグ味・渋味の抑制)を確認した実験である。 Example 14 is an experiment for confirming the unpleasant taste reduction effect (suppression of egg taste and astringency) of red bean paste.
まず、生あんに同量の水を加えて均一化させたスラリーを準備した。次に、前記スラリーに、同量のパノース20重量%含有糖組成物、トレハロース、マルトース、及び砂糖を加えて煮詰め、小豆こしあん試作品を調製した。そして、上記の調製品について実施例2と同様の評価基準により官能評価を行った。その結果を表14に示す。 First, a uniform slurry was prepared by adding the same amount of water to raw bean paste. Next, the same amount of sugar composition containing 20% by weight of panose, trehalose, maltose, and sugar was added to the slurry and boiled to prepare a red bean kojian prototype. And sensory evaluation was performed about the said preparation by the evaluation criteria similar to Example 2. FIG. The results are shown in Table 14.
表14に示すとおり、マルトースには、砂糖と同等のエグ味・渋味が残っており、不快味低減効果はほとんど確認できなかった。トレハロースを添加したものは、ある程度の不快味低減効果が見られたが、トレハロース特有の風味を有していた。それに対し、パノース20重量%含有糖組成物は、トレハロースよりもさらに良好な不快味低減効果が見られた。 As shown in Table 14, the maltose had an egg taste and astringency equivalent to sugar, and an unpleasant taste reduction effect could hardly be confirmed. The addition of trehalose had a certain unpleasant taste reducing effect, but had a flavor peculiar to trehalose. On the other hand, the sugar composition containing 20% by weight of panose showed an even better unpleasant taste reducing effect than trehalose.
パノースは、小豆こしあんのおいしさを損なうことなく、エグ味・渋味などの不快味を低減することができる点で、トレハロースよりも有利性がある。また、上記の結果より、パノース20重量%含有糖組成物は、小豆こしあん以外の、例えば、灰汁を有する食品などに対しても、適用可能であると考えられる。 Panose is more advantageous than trehalose in that it can reduce unpleasant tastes such as egg taste and astringency without detracting from the deliciousness of red bean paste. From the above results, it is considered that the sugar composition containing 20% by weight of panose can be applied to foods having lye other than red bean paste.
実施例15は、全粒粉クッキーの不快臭低減効果(ふすま臭の抑制)を確認した実験である。 Example 15 is an experiment in which the effect of reducing unpleasant odor (suppression of bran odor) of whole grain cookies was confirmed.
全粒粉クッキーは、以下の手順により製造した。なお、糖質としては、(1)パノース20重量%含有糖組成物40gと砂糖40g、(2)トレハロース40gと砂糖40g、(3)マルトース40gと砂糖40g、(4)砂糖のみ80g(対照)、をそれぞれ配合した。 Whole grain cookies were produced by the following procedure. In addition, as sugar, (1) Panose 20 weight% containing sugar composition 40g and sugar 40g, (2) Trehalose 40g and sugar 40g, (3) Maltose 40g and sugar 40g, (4) Only sugar 80g (control) , Respectively.
まず、電子レンジで柔らかくした無塩バター110gをクリーム状になるまでハンドミキサーで練った後、前記糖質計80gを3回に分けて練り混ぜながら添加した。次に、バニラエッセンス100μgを加え、卵黄29gを3回に分けて添加し、練り混ぜた。そして、薄力粉124g、全粒粉31g、ベーキングパウダー2.5g(小さじ1)を加えて軽く混ぜ合わせた後、軽く混ぜ合わせ、直径3cmのボール状に丸めた。なお、全材料の合計重量は、約377gだった。次に、オーブンペーパー上に並べ、軽く押しつぶしてほぼ均一の厚さにした後、160℃に予備加熱したオーブンで約30分焼成し、全粒粉クッキー試作品を製造した。 First, 110 g of unsalted butter softened with a microwave oven was kneaded with a hand mixer until it became creamy, and then 80 g of the saccharide meter was added in three portions while mixing. Next, 100 μg of vanilla essence was added, and 29 g of egg yolk was added in three portions and kneaded. Then, 124 g of weak flour, 31 g of whole grain powder, and 2.5 g (1 teaspoon) of baking powder were added and lightly mixed, then lightly mixed and rolled into a 3 cm diameter ball. The total weight of all materials was about 377 g. Next, they were arranged on oven paper, lightly crushed to an almost uniform thickness, and then baked in an oven preheated to 160 ° C. for about 30 minutes to produce a whole grain cookie prototype.
上記の試作品について実施例2と同様の評価基準により官能評価を行った。結果を表15に示す。 The above prototype was subjected to sensory evaluation according to the same evaluation criteria as in Example 2. The results are shown in Table 15.
その結果、全調製品についてふすま臭は残っていたが、パノース20重量%含有糖組成物及びトレハロースでは、有意の不快臭(ふすま臭)低減効果が確認できた。但し、トレハロースは、不快臭低減効果が確認できたものの、クッキー焼成後の香気が大きく損なわれていた。 As a result, the bran odor remained in all the prepared products, but the sugar composition and trehalose containing 20% by weight of panose confirmed a significant effect of reducing the unpleasant odor (bran odor). However, although trehalose was confirmed to have an unpleasant odor reducing effect, the aroma after baking the cookies was greatly impaired.
従って、パノースは、クッキーの風味・香味を損なうことなく、不快臭を低減することができる点で、トレハロースよりも有利性がある。なお、上記の結果より、パノース20重量%含有糖組成物は、全粒粉クッキー以外の、全粒粉を用いた穀物臭が強い食品などに対しても、不快臭低減効果が期待でき、適用可能であると考えられる。 Therefore, panose has an advantage over trehalose in that it can reduce unpleasant odor without impairing the flavor and flavor of the cookie. From the above results, the panose 20% by weight sugar composition can be expected to have an unpleasant odor reduction effect and can be applied to foods with a strong grain odor using whole grain powder other than whole grain cookies. Conceivable.
実施例16は、食パンに対する不快臭低減効果(酵母臭などの抑制)を確認した実験である。 Example 16 is an experiment in which an unpleasant odor reduction effect (suppression of yeast odor and the like) on bread was confirmed.
試作品の食パンは、以下の手順により製造した。なお、糖質としては、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトースを用いた。 The prototype bread was produced according to the following procedure. As sugars, panose 20% by weight sugar composition, trehalose and maltose were used, respectively.
まず、小麦粉350g、砂糖15g、乾燥酵母30g、イーストフード1.5g、食塩10g、脱脂粉乳15g、水210gを混合し、中種を製造した。次に、各糖質を25g添加した後、ミキサーで15分混捏した。次に、混捏したパン生地を分割して丸め、中間生地を製造した。次に、中間生地をポリエチレンの袋に入れ、急速冷凍後、−30℃の冷凍庫に一週間保管した。一週間の冷凍保管の後、ドウコンディショナーを用いて、解凍・発酵した。そして、発酵させた生地を分割し、ホイロで再発酵させた後、焼成してパンを製造した。 First, 350 g of wheat flour, 15 g of sugar, 30 g of dry yeast, 1.5 g of yeast food, 10 g of salt, 15 g of skim milk powder, and 210 g of water were mixed to produce a medium seed. Next, 25 g of each saccharide was added and then kneaded with a mixer for 15 minutes. Next, the mixed dough was divided and rolled to produce an intermediate dough. Next, the intermediate dough was put in a polyethylene bag, rapidly frozen, and stored in a freezer at -30 ° C for one week. After freezing for one week, it was thawed and fermented using a dough conditioner. The fermented dough was divided and re-fermented with a proofer, and then baked to produce bread.
上記の試作品について実施例2と同様の評価基準により官能評価を行った。結果を表16に示す。 The above prototype was subjected to sensory evaluation according to the same evaluation criteria as in Example 2. The results are shown in Table 16.
表16に示すとおり、トレハロースでは、有意の不快臭(酵母臭)低減効果が確認できたものの、パン焼成後の好ましい風味が損なわれており、総合評価は「×」となった。それに対し、パノース20重量%含有糖組成物は、不快臭(酵母臭)低減作用を有し、パン焼成後も好ましい風味を保持していた。なお、マルトースは、パン焼成後のよい香りは保持されていたが、不快臭低減効果はほとんど確認できなかった。 As shown in Table 16, with trehalose, although a significant unpleasant odor (yeast odor) reduction effect could be confirmed, the preferred flavor after baking was impaired, and the overall evaluation was “x”. On the other hand, the sugar composition containing 20% by weight of panose had an unpleasant odor (yeast odor) reducing action and maintained a favorable flavor even after baking. In addition, maltose maintained a good scent after baking, but almost no unpleasant odor reduction effect could be confirmed.
以上より、パノースは、パンの風味・香味を損なうことなく、酵母臭などの不快臭を顕著に低減することができる点で、トレハロースよりも有利性がある。なお、上記の結果より、パノース20重量%含有糖組成物は、食パン以外の、酵母など菌体を用いる飲食品などに対しても、不快臭改善効果が期待でき、適用可能であると考えられる。 From the above, panose is more advantageous than trehalose in that it can significantly reduce unpleasant odors such as yeast odor without impairing the flavor and flavor of bread. From the above results, the panose 20% by weight-containing sugar composition can be expected to have an effect of improving unpleasant odors for foods and drinks using fungal cells such as yeast other than bread. .
実施例17は、大豆タンパク質を使用したハンバーグの不快臭低減効果(大豆臭の抑制)を確認した実験である。 Example 17 is an experiment in which hamburger's unpleasant odor reduction effect (suppression of soybean odor) using soybean protein was confirmed.
試作品のハンバーグは、以下の手順により製造した。なお、糖質としては、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、砂糖(対照)を用いた。 The prototype hamburger was manufactured according to the following procedure. As sugars, panose 20% by weight-containing sugar composition, trehalose, maltose, and sugar (control) were used, respectively.
まず、大豆タンパク質に戻し水を加えて水戻しした。次に、水戻しした大豆タンパク質(大豆タンパク質の配合量4%、戻し水の含有量9%)、合挽き肉(配合量39%)、ラード(同6%)、水(同12%)、たまねぎ(同15%)、卵白(同4%)、パン粉(同3%)、マッシュポテト(同2%)、調味料(同3%)、前記の糖質(同3%)を、混合・成形後、焼成して、ハンバーグ試作品を製造した。 First, the soy protein was reconstituted with water. Next, reconstituted soy protein (4% soy protein content, 9% reclaimed water content), ground meat (39% content), lard (6%), water (12%), onion (15%), egg white (4%), bread crumbs (3%), mashed potatoes (2%), seasonings (3%), sugars (3%) after mixing and molding Baked to produce a hamburger prototype.
上記の試作品について実施例1と同様の評価基準により官能評価を行った。結果を表17に示す。 The above prototype was subjected to sensory evaluation according to the same evaluation criteria as in Example 1. The results are shown in Table 17.
表17に示すとおり、マルトースは、砂糖を用いたものとほぼ同等で、大豆臭が強く、不快臭低減効果は確認できなかった。トレハロースでは、ある程度の不快臭低減効果が見られたが、パノース20重量%含有糖組成物のほうが、明らかな不快臭低減効果を確認できた。なお、上記の結果より、パノース20重量%含有糖組成物は、ハンバーグ以外の、大豆たんぱく質を用いる食品などに対しても、不快臭低減効果が期待でき、適用可能であると考えられる。 As shown in Table 17, maltose was almost the same as that using sugar, had a strong soybean odor, and an unpleasant odor reduction effect could not be confirmed. Trehalose was found to have a certain unpleasant odor reducing effect, but the sugar composition containing 20% by weight of panose confirmed a clear unpleasant odor reducing effect. From the above results, it is considered that the sugar composition containing 20% by weight of panose can be applied to foods using soy protein other than hamburgers and can be expected to have an unpleasant odor reduction effect.
実施例18は、カスタードプリンの不快臭低減効果(卵臭の抑制)を確認した実験である。 Example 18 is an experiment in which the effect of reducing the unpleasant odor of custard pudding (suppression of egg odor) was confirmed.
試作品のカスタードプリンは、以下の手順により製造した。なお、糖質としては、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、砂糖(対照)を用いた。 The prototype custard pudding was manufactured by the following procedure. As sugars, panose 20% by weight-containing sugar composition, trehalose, maltose, and sugar (control) were used, respectively.
まず、湯煎で60℃に温めた牛乳480gに、殺菌液卵(卵の中身を割りほぐしたもの)240gを撹拌しながら徐々に加え、均一化した。次に、香料2gと糖質溶液(前記糖質75gを調合水130gに溶解させたもの)をさらに加え、均一化した後、調製液を三回裏ごしし、アルミカップに注入した。次に、予めオーブン皿に水を張ったうえで、160℃、10分間予熱したオーブンに、アルミカップを入れ、約30分間焼成し、カスタードプリン試作品を製造した。試作品は、4℃の冷蔵庫で保存した。 First, 240 g of a sterilized liquid egg (obtained by breaking the contents of an egg) was gradually added to 480 g of milk warmed to 60 ° C. with a hot water bath, and the mixture was homogenized. Next, 2 g of fragrance and a saccharide solution (75 g of the saccharide dissolved in 130 g of prepared water) were further added and homogenized, and then the prepared solution was lined three times and poured into an aluminum cup. Next, after pre-watering the oven dish, the aluminum cup was put into an oven preheated at 160 ° C. for 10 minutes and baked for about 30 minutes to produce a custard pudding prototype. The prototype was stored in a refrigerator at 4 ° C.
上記の試作品について実施例1と同様の評価基準により官能評価を行った。結果を表18に示す。 The above prototype was subjected to sensory evaluation according to the same evaluation criteria as in Example 1. The results are shown in Table 18.
表18に示すとおり、マルトースは、砂糖を用いたものとほぼ同等で、卵臭が強く、不快臭低減効果は確認できなかった。トレハロースでは、ある程度の不快臭低減効果が見られたが、パノース20重量%含有糖組成物のほうが、顕著な不快臭低減効果を確認できた。なお、上記の結果より、パノース20重量%含有糖組成物は、カスタードプリン以外の、卵を用いた食品などに対しても、不快臭低減効果が期待でき、適用可能であると考えられる。 As shown in Table 18, maltose was almost the same as that using sugar, had a strong egg odor, and an unpleasant odor reduction effect could not be confirmed. With trehalose, an unpleasant odor reducing effect was observed to some extent, but the sugar composition containing 20% by weight of panose was able to confirm a remarkable unpleasant odor reducing effect. From the above results, it is considered that the sugar composition containing 20% by weight of panose can be applied to foods using eggs other than custard pudding and can be applied.
実施例19は、ペプチド入り乳飲料に対する不快味低減効果(苦味、エグ味などの抑制)を示した実験である。 Example 19 is an experiment showing an effect of reducing unpleasant taste (inhibition of bitterness, taste, etc.) on a peptide-containing milk beverage.
ペプチド入り乳飲料は、以下の手順により調製した。なお、糖質としては、それぞれ、パノース20重量%含有糖組成物、トレハロース、マルトース、砂糖(対照)を用いた。 A milk beverage containing peptide was prepared by the following procedure. As sugars, panose 20% by weight-containing sugar composition, trehalose, maltose, and sugar (control) were used, respectively.
まず、ペクチン0.05kgと砂糖0.3kgを水10kgに溶解した後、各糖質0.3kgを添加・溶解して冷却した。次に、その溶液に生乳(脂肪3.6%、無脂乳固形8.5%)5kg添加し、ホモミキサーで充分混合した後、濃縮りんご果汁1kg、ペプチド0.2kgを添加した。そして、25%クエン酸溶液でpH3.9に調製し、酸性乳飲料懸濁液を得た。次に、この懸濁液を高圧ホモジナイザーで均質化処理し、UHTプレート殺菌機で、120℃、2秒間殺菌処理した後、再度均質化処理し、ペプチド入り乳飲料を製造した。製造したペプチド入り乳飲料は、5℃で冷却し、保存した。 First, 0.05 kg of pectin and 0.3 kg of sugar were dissolved in 10 kg of water, and then 0.3 kg of each saccharide was added and dissolved, followed by cooling. Next, 5 kg of raw milk (3.6% fat, non-fat milk solid 8.5%) was added to the solution, and after thoroughly mixing with a homomixer, 1 kg of concentrated apple juice and 0.2 kg of peptide were added. Then, the pH was adjusted to 3.9 with a 25% citric acid solution to obtain an acidic milk beverage suspension. Next, the suspension was homogenized with a high-pressure homogenizer, sterilized with a UHT plate sterilizer at 120 ° C. for 2 seconds, and then homogenized again to produce a peptide-containing milk beverage. The produced milk drink containing peptide was cooled at 5 ° C. and stored.
なお、ペプチド入り乳飲料に用いたペプチドは、市販の大豆ペプチド(商品名「ハイニュート−PM」、不二製油株式会社製)、とうもろこしペプチド(商品名「ペプチーノ」、日本食品化工株式会社製)と、とうもろこしタンパク質、大豆タンパク質、小麦タンパク質、ジャガイモタンパク質、卵白タンパク質の各タンパク質から調製したペプチド粉末を用いた。 Peptides used in milk beverages containing peptides are commercially available soy peptide (trade name “High New-PM”, manufactured by Fuji Oil Co., Ltd.), corn peptide (trade name “Peptuccino”, manufactured by Nippon Shokuhin Kako Co., Ltd.). Peptide powders prepared from corn protein, soybean protein, wheat protein, potato protein, and egg white protein were used.
ペプチド粉末の調製手順は、次のとおりである。前記各タンパク質1kgを10Lの水に懸濁した後、水酸化ナトリウムを添加してpH8.0に調製した。次に、懸濁液にアルカリプロテアーゼ(商品名「アルカラーゼ」、ノポザイムズ社製)を、対固形分0.1%添加し、60℃、15時間、タンパク質の部分分解を行った。次に、塩酸でpH7に調製した後、中性プロテアーゼ(商品名「プロテアーゼA」、天野製薬株式会社製)により、5時間酵素分解した。次に、塩酸でpH4に調製した後、酸性プロテアーゼ(商品名「スミチーム」、新日本化学株式会社製)により、50℃、5時間、酵素分解した。そして、得られたタンパク分解液を、固液分離・活性炭処理後、イオン精製し、噴霧乾燥させて、各種ペプチド粉末を調製した。 The procedure for preparing the peptide powder is as follows. After 1 kg of each protein was suspended in 10 L of water, sodium hydroxide was added to adjust the pH to 8.0. Next, alkaline protease (trade name “Alcalase”, manufactured by Nopozymes) was added to the suspension in a solid content of 0.1%, and the protein was partially decomposed at 60 ° C. for 15 hours. Next, after adjusting the pH to 7 with hydrochloric acid, it was enzymatically degraded with a neutral protease (trade name “Protease A”, manufactured by Amano Pharmaceutical Co., Ltd.) for 5 hours. Next, after adjusting to pH 4 with hydrochloric acid, enzymatic degradation was performed at 50 ° C. for 5 hours with an acidic protease (trade name “Sumiteam”, manufactured by Shin Nippon Chemical Co., Ltd.). The obtained proteolytic solution was subjected to solid-liquid separation and activated carbon treatment, ion purified, and spray-dried to prepare various peptide powders.
上記の手順により調製したペプチド入り乳飲料について、実施例1と同様の評価基準により官能評価を行った。その結果、ペプチドの種類によって、不快味の程度は異なったものの、糖質ごとの不快味低減効果は、ほぼ同等であった。とうもろこしペプチドを用いたペプチド入り乳飲料の結果を表19に示す。 The peptide-containing milk beverage prepared by the above procedure was subjected to sensory evaluation according to the same evaluation criteria as in Example 1. As a result, although the degree of unpleasant taste varied depending on the type of peptide, the unpleasant taste reducing effect for each saccharide was almost the same. Table 19 shows the results of milk beverages containing peptides using corn peptides.
表19に示すとおり、砂糖及びマルトースを用いた場合、不快味低減作用は確認できず、トレハロースを用いた場合も、顕著な不快味低減作用は確認できなかった。また、トレハロースを用いた場合、乳飲料が独特な味に変化した。それに対し、パノースは、苦味・エグ味などの不快味を顕著に低減し、総合評価も「○」であった。 As shown in Table 19, when sugar and maltose were used, no unpleasant taste reducing action could not be confirmed, and even when trehalose was used, no significant unpleasant taste reducing action could be confirmed. In addition, when trehalose was used, the milk beverage changed to a unique taste. On the other hand, panose significantly reduced unpleasant tastes such as bitterness and egg taste, and the overall evaluation was “◯”.
従って、パノースは、ペプチド入り乳飲料本来の風味を損なうことなく、苦味・エグ味などの不快味を低減できることが分かった。なお、この実験より、本発明に係るパノース20重量%含有糖組成物は、アミノ酸やペプチドを配合した飲食品や乳製品などに対しても、広く適用可能であると考えられる。 Therefore, it was found that panose can reduce unpleasant tastes such as bitterness and egg taste without impairing the original flavor of milk beverages containing peptides. From this experiment, it is considered that the 20% by weight panose-containing saccharide composition according to the present invention can be widely applied to foods and drinks and dairy products containing amino acids and peptides.
実施例20は、パノース以外の糖類でも、パノースと同様の不快味・不快臭低減効果がみられるかどうかを調べた実験である。 Example 20 is an experiment in which it was investigated whether saccharides other than panose can exhibit the same unpleasant taste / unpleasant odor reducing effect as panose.
実験は、以下の手順で行った。まず、市販の有機豆乳に、表18の中列に列記した糖類をそれぞれ10重量%(固形分換算)になるように添加し、4℃で1日保存した。そして、各豆乳調製品について、実施例1と同様の評価基準により官能評価を行った。 The experiment was performed according to the following procedure. First, sugars listed in the middle row of Table 18 were added to commercially available organic soymilk so that each was 10 wt% (in terms of solid content) and stored at 4 ° C. for 1 day. Each soymilk preparation was subjected to sensory evaluation according to the same evaluation criteria as in Example 1.
なお、用いた糖質は、単糖類(その還元物も含む)として、グルコース(和光純薬株式会社製、製品コード587−41505)、フルクトース(和光純薬株式会社製、製品コード127−02765)、アラビノース(和光純薬株式会社製、製品コード041−00596)、キシロース(和光純薬株式会社製、製品コード248−00305)、ソルビトール(和光純薬株式会社製、製品コード198−03755)、キシリトール(和光純薬株式会社製、製品コード248−00545)、エリスリトール(和光純薬株式会社製、製品コードA15813)、二糖類(その還元物も含む)として、トレハロース(和光純薬株式会社製、製品コード201−02253)、コージビオース(和光純薬株式会社製、製品コード111−00481)、ニゲロース(和光純薬株式会社製、製品コード146−06431)、マルトース(和光純薬株式会社製、製品コード130−00615)、イソマルトース(和光純薬株式会社製、製品コード632−04081)、マルチトール(和光純薬株式会社製、製品コード155316)、イソマルチトール(和光純薬株式会社製、製品コード631−04051)、三糖類(その還元物も含む)として、マルトトリオース(和光純薬株式会社製、製品コード131−06544、以下同じ)、イソマルトトリオース(生化学工業株式会社製、製品コード400473、以下同じ)、パノース(生化学工業株式会社製、製品コード400554)、マルトトリイトール(生化学工業株式会社製、製品コード400520)、イソマルトトリイトール、パニトール、四糖類(その還元物も含む)として、マルトテトラオース(生化学工業株式会社製、製品コード400519)、イソマルトテトラオース(生化学工業株式会社製、製品コード400475)、マルトテトライトール(生化学工業株式会社製、製品コード400518)、イソマルトテトライトールである。 In addition, the used saccharide | sugar is glucose (a Wako Pure Chemical Industries Ltd. make, product code 587-41505), fructose (Wako Pure Chemical Industries Ltd. make, product code 127-02765) as a monosaccharide (including the reduced product). Arabinose (manufactured by Wako Pure Chemical Industries, Ltd., product code 041-00596), xylose (manufactured by Wako Pure Chemical Industries, Ltd., product code 248-00305), sorbitol (manufactured by Wako Pure Chemical Industries, Ltd., product code 198-03755), xylitol (Wako Pure Chemical Industries, Ltd., product code 248-00545), erythritol (Wako Pure Chemical Industries, Ltd., product code A15813), disaccharide (including its reduced product), trehalose (Wako Pure Chemical Industries, Ltd., product) Code 201-02253), Kojibiose (manufactured by Wako Pure Chemical Industries, Ltd., product code 111-00) 81), nigerose (manufactured by Wako Pure Chemical Industries, Ltd., product code 146-06431), maltose (manufactured by Wako Pure Chemical Industries, Ltd., product code 130-00615), isomaltose (manufactured by Wako Pure Chemical Industries, Ltd., product code 632-04081) ), Maltitol (manufactured by Wako Pure Chemical Industries, Ltd., product code 155316), isomaltitol (manufactured by Wako Pure Chemical Industries, Ltd., product code 631-04051), trisaccharide (including its reduced product), maltotriose ( Wako Pure Chemical Industries, Ltd., product code 131-06544 (hereinafter the same), isomalt triose (Seikagaku Corporation, product code 4000047, the same shall apply hereinafter), Panose (Seikagaku Corporation, product code 400554) , Maltotriitol (manufactured by Seikagaku Corporation, product code 400520), isomalt As lyitol, panitol, and tetrasaccharide (including reduced products thereof), maltotetraose (manufactured by Seikagaku Corporation, product code 400519), isomalttetraose (manufactured by Seikagaku Corporation, product code 400475), maltote Trytol (manufactured by Seikagaku Corporation, product code 400518), isomaltotetritol.
イソマルトトリイトールは、Starke 26 307-312 (1974)に記載された方法に従い、前記イソマルトトリオースを、ラニーニッケル添加、加圧条件下、水素で還元し、調製した。パニトールは、前記と同様の方法により、前記パノースを、ラニーニッケル添加、加圧条件下、水素で還元し、調製した。イソマルトテトライトールは、前記と同様の方法により、前記イソマルトテトラオースを、ラニーニッケル添加、加圧条件下、水素で還元し、調製した。結果を表20に示す。 Isomaltotriitol was prepared according to the method described in Starke 26 307-312 (1974) by reducing the isomaltotriose with hydrogen under the addition of Raney nickel and under pressurized conditions. Panitol was prepared in the same manner as described above by reducing the panose with hydrogen under the addition of Raney nickel and under pressurized conditions. Isomaltotetriitol was prepared in the same manner as described above by reducing the isomalttetraose with hydrogen under the addition of Raney nickel and under pressurized conditions. The results are shown in Table 20.
表20に示すとおり、単糖類では、ほとんど不快味・不快臭低減効果は見られず、二糖類でも、弱い不快味・不快臭低減効果が見られるのみであった。一方、三糖類の中では、パノースの効果が最も高かったが、その他の糖類(グルコースを構成糖とした三糖類、その還元物)でも、高い不快味・不快臭低減効果が見られた。パノース及びパニトールでは、低甘味で素材の持ち味を活かしつつ大豆独特の不快臭を低減できており、極めて良好な結果となった。それに対し、四糖類は、弱い不快味・不快臭低減効果しか見られなかった。 As shown in Table 20, almost no unpleasant taste / unpleasant odor reduction effect was observed with monosaccharides, and only a weak unpleasant taste / unpleasant odor reduction effect was observed with disaccharides. On the other hand, among the trisaccharides, the effect of panose was the highest, but other saccharides (trisaccharides containing glucose as a constituent saccharide and its reduced product) also showed a high unpleasant taste / unpleasant odor reducing effect. Panose and panitol were able to reduce the unpleasant odor peculiar to soybeans while taking advantage of the taste of the material with low sweetness, and the results were extremely good. On the other hand, tetrasaccharides showed only a weak unpleasant taste / unpleasant odor reducing effect.
上記の結果は、三糖類の立体構造が不快味・不快臭原因物質を包み込むようにして結合する際に、最も適した大きさ・形状であることを示唆している。また、三糖類の中でも、パノースの不快味・不快臭低減効果が最も高いのは、パノースの有する弓形の立体形状が、不快味・不快臭原因物質を包み込むのに最も適しているためと考えられる。なお、四糖類の不快味・不快臭低減効果が低いのは、四糖類の場合、糖鎖が長すぎてパノースのような弓形形状にはならず、不快味・不快臭原因物質を包み込むのに適した立体形状を持たないためであるト推測する。 The above results suggest that the three-dimensional structure of the trisaccharide is the most suitable size and shape when bound so as to wrap around the substances that cause unpleasant taste and unpleasant odor. In addition, among the trisaccharides, panose has the highest effect of reducing the unpleasant taste and unpleasant odor. It is thought that the panoid three-dimensional shape of panose is most suitable for enveloping substances that cause unpleasant taste and unpleasant odor. . The tetrasaccharides are less effective in reducing the unpleasant taste and unpleasant odor, because in the case of tetrasaccharides, the sugar chain is too long and does not have an arcuate shape like panose. This is because it does not have a suitable three-dimensional shape.
また、マルトトリオース、イソマルトトリオース、パノースをなど量ずつ混合した糖質でも、不快味・不快臭低減効果を示すことが示された。即ち、不快味・不快臭低減効果を有する糖質を組み合わせた場合でも、同様の効果を得ることができた。 Moreover, it was shown that the carbohydrate which mix | blended maltotriose, isomaltotriose, panose, etc. in the amounts each shows the effect of reducing unpleasant taste and unpleasant odor. That is, even when carbohydrates having an unpleasant taste / unpleasant odor reducing effect were combined, the same effect could be obtained.
以上より、パノース以外の三糖類も、一定の不快味・不快臭低減効果を有することが分かった。従って、パノース以外の三糖類を食品などに添加することによっても、一定の不快味・不快臭低減効果を得ることができると推定できる。 From the above, it was found that trisaccharides other than panose also have a certain unpleasant taste / unpleasant odor reducing effect. Therefore, it can be presumed that a certain unpleasant taste / unpleasant odor reduction effect can be obtained by adding trisaccharides other than panose to foods and the like.
本発明は、グルコースを構成糖とした三糖類とその還元物を不快味・不快臭低減有効成分として使用できるので、産業上の利用可能性がある。 INDUSTRIAL APPLICABILITY Since the present invention can use a trisaccharide having glucose as a constituent sugar and its reduced product as an effective component for reducing unpleasant taste and unpleasant odor, it has industrial applicability.
また、グルコースを構成糖とした三糖類とその還元物を不快味・不快臭低減の有効成分として含有する糖組成物は、不快味・不快臭低減作用を持つ甘味料などとして産業上有用である。 In addition, a sugar composition containing a trisaccharide composed of glucose as a constituent sugar and its reduced product as an active ingredient for reducing unpleasant taste and unpleasant odor is industrially useful as a sweetener having an effect of reducing unpleasant taste and unpleasant odor. .
さらに、本発明に係る糖組成物は、飲食物に添加して使用することができるので、産業上有用である。 Furthermore, since the saccharide | sugar composition which concerns on this invention can be added and used for food and drink, it is industrially useful.
Claims (2)
パノースおよび/またはパニトールを20%以上含有する糖組成物を、不快味および/または不快臭を有する飲食物に添加することにより、該飲食物の不快味および/または不快臭を低減させることを特徴とする不快味および/または不快臭低減方法。 A method for reducing unpleasant taste and / or unpleasant odor of food and drink by adding to food and drink,
By adding a sugar composition containing 20% or more of panose and / or panitol to a food or drink having an unpleasant taste and / or unpleasant odor, the unpleasant taste and / or unpleasant odor of the food or drink is reduced. An unpleasant taste and / or unpleasant odor reducing method .
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