JP3641408B2 - Automatic analyzer and automatic analysis method - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to an analysis such as a biochemical test and an immunoserologic test, and relates to an automatic analyzer that sets and measures a sample container containing various types of samples in a sample erection mechanism.
[0002]
[Prior art]
The rack on which the samples arranged by the automatic analyzer operator in the order of measurement request and measurement registration are transported to the sample dispensing position of the analyzer and positioned, and then the sample probe dispenses the sample a predetermined number of times. When the dispensing of one sample is completed, the rack moves by one sample from the loading side to the storage side, and the dispensing operation for the next sample is started again. In this way, the same operation is repeated and the sample container advances by one sample. Conventionally, when the dispensing operation for one sample is completed, the rack always advances by one sample in order to dispense the next new sample. In the case of measuring the reproducibility of the measurement value with a measurement number of 100, 100 sample containers and samples to be put in 100 sample containers were prepared. This is because there is an input restriction that the same rack number and position number cannot be continuously input to the request information in order to minimize mistakes in the sample dispensing operation due to input errors in the request information of the operator. . Although there was a method of creating a screen dedicated to evaluation measurement, it was impossible with the conventional apparatus because the memory was taken up for operation control of the apparatus and the usable memory capacity was limited.
[0003]
Japanese Patent Application Laid-Open No. 9-96643 describes that when the same specimen is dispensed into a microplate, the same liquid inhalation auxiliary tube is used.
[0004]
[Problems to be solved by the invention]
The sample used for quality control of the apparatus put in 100 sample containers is expensive, and therefore, a lot of expensive samples have to be prepared. This is because there is an input restriction that the same rack number and position number cannot be continuously input to the request information in order to minimize mistakes in the sample dispensing operation due to input errors in the request information of the operator. . Although there was a method of creating a screen dedicated to evaluation measurement, it was impossible with the conventional apparatus because the memory was taken up for operation control of the apparatus and the usable memory capacity was limited.
[0005]
Since automatic analyzers for clinical tests are required to maintain a high level of measurement accuracy, reproducibility and accuracy are regularly checked in the laboratory. In particular, at the time of installation of the apparatus, the check work of the apparatus manufacturer, reagent manufacturer, and inspection facility operator is performed over several weeks. Conventionally, it took time and labor for experiments such as setting a sample container, and it took a long time to check reproducibility. There were many work mistakes because it took time. In addition, a large amount of waste such as used sample containers and quality control samples was generated.
[0006]
In view of this point, the present invention enables frequent dispensing of a sample from the same sample container without complicating the structure of the apparatus, so that the measurement accuracy of an automatic analyzer and the examination of reagents are frequently used. Automatic analyzer and automatic analysis method that can easily perform simultaneous reproducibility measurement, linearity measurement, sample coexistence substance influence measurement, sample probe / reagent probe / stirring rod / reaction vessel carryover check, etc. The purpose is to provide.
[0007]
[Means for Solving the Problems]
In order to solve the above problems, the present invention has the following configuration.
[0008]
A screen for basic performance test items has been added to the routine operation screen, with buttons for selecting experiments for simultaneous reproducibility, linearity, coexisting substances, stirring bar carryover, reaction vessel carryover, reagent probe carryover, and sample probe carryover. By pressing this button, the screen for setting the conditions for each experiment appears, and the request information can be entered.
[0009]
When a laboratory technician brings a sample for measuring accuracy evaluation, continuous measurement from the same sample container is not possible in the general sample measurement mode, so the operator changes the screen to the evaluation mode and requests measurement from the evaluation mode screen. do. The request information is stored in the memory, and the operator sets the rack on which the sample is placed and starts the apparatus. The apparatus first reads the rack number or sample ID and detects the presence or absence of the sample container. If the read rack number or sample ID matches the request information stored in the memory, the items are sampled according to the request information. In the conventional apparatus, the same rack number, position number, and sample ID cannot be continuously input to the rack number, the position number on the rack, and the sample ID input area. In the present invention, the same number can be input to the rack number, position number, or sample ID input area.
[0010]
The sample probe repeats the sample dispensing operation every fixed cycle, and the conveyor belt positions the rack, so if you want to repeat the dispensing operation of the same item from the same sample container, the same rack number and the same position By inputting the number of necessary samples from the screen, the sample dispensing operation can be repeated from the same position number as many times as necessary.
[0011]
Normally, the rack has only progressed from the rack loading side to the rack storage side, but holding the rack by holding the rack with two claws allows it to move in the opposite direction, as in sample carryover measurement. In addition, the sample can be reused for repeated measurement of the same pattern in which a low concentration sample is measured several times after a high concentration sample.
[0012]
The normally loaded rack is discharged when the measurement for one rack is completed, and the next rack is introduced into the sample dispensing area and positioned. In the present invention, for example, when one rack is five sample racks and only one rack is loaded, if the rack number designation of the sixth sample is the same, the rack is returned to the loading side using the retest line, and the rack The first position sample is recognized as the sixth sample and measured. The same rack number was input continuously for the desired number of measurements so that the rack could be circulated. A plurality of racks having different rack numbers can be circulated by interrupting racks having different rack numbers.
[0013]
In order to facilitate repeated measurement of the same sample in the same sample container, a holder is installed on the upper part of the transport line in the sample dispensing area of the device to install a container that can hold a large amount of sample at one time. A sample was set so that the same sample could be repeatedly measured.
[0014]
DETAILED DESCRIPTION OF THE INVENTION
Embodiments of the present invention will be described below with reference to the drawings.
[0015]
FIG. 1 is a diagram showing a rack transport and storage method of the automatic analyzer shown in the present embodiment, and the basic operation will be described next.
[0016]
The automatic analyzer includes a plurality of racks, a loading unit 1 provided with a sample erection device, two analysis units 2, an analysis unit 3, a transport line 4, and a rack storage unit provided in the sample dispensing device and the reagent dispensing device. It is composed of five. Each rack has a plurality of positions, into which sample containers are placed.
[0017]
A sample is put in a sample container 6 and set in a rack 7. The rack 7 is attached to the rack loading unit 1. When the start key is pressed after inputting and registering the analysis items of each sample 6, the apparatus continues the reset operation and pulls the rack with the transfer claws to start the rack transfer. The rack 7 sent out from the rack loading unit 1 is read by the rack number or sample ID reader 10 with the rack number or sample ID. The apparatus determines whether or not the rack corresponding to the input information has been inserted, and when the rack arrives, transports it to the sample dispensing area. The top sample among the plurality of samples placed on the rack is positioned at the sample dispensing position on the main line 8. The sample in the sample container 6 is collected and discharged to the reaction container by a set amount, and the collection and discharge are repeated for the requested number of tests. The rack for which the sample has been collected is collected in the rack storage unit 5 and the measurement is completed.
[0018]
FIG. 2 shows a flowchart of processing from receiving a sample to completing the measurement.
[0019]
The operator sets a sample to be measured (S101). According to the measurement purpose of the sample to be measured, the measurement mode is selected from “Emergency”, “General”, and “Evaluation” on the apparatus screen (S102), and request information specific to each sample is input. Sample type (S103) indicating whether the sample is serum, urine or other, sample number (S104) which is the serial number of the sample, rack number (S105) where the sample is set, position number (S106), sample container The cup type (S107) indicating the type and the measurement request item (S108) are sequentially input, and the apparatus is started (S109). The apparatus reads the rack number and sample ID (S110), detects the presence or absence of a sample container (S111), and dispenses the sample (S113). During the sample dispensing operation, the rack number and position number of the next sample number are checked, and when the rack number and the position number input value are the same (match), the rack feeding operation is not performed (S112). The sample is stopped at the same position, and the sample dispensing operation is performed on the sample at the same position (S113). The measurement ends with the output of the result (S114).
[0020]
3, 4, and 5 are screen examples of the apparatus that serves as both a measurement mode switch and request information input. FIG. 3 shows an example in which the rack number 303 and the position number 304 are used for sample identification, and FIG. 4 shows an example in which the sample ID number 401 is used for sample identification. In addition, when requesting measurement items, a method in which a plurality of the same items cannot be requested for a single sample has been employed, but FIG. 5 which is an embodiment of the present invention has the same software, and the same An example of a screen that allows multiple requests for the same item to the sample and enables repeated measurement of the same item is shown.
[0021]
This will be specifically described with reference to the screen example of FIG. AST is measured 5 times from sample number 1 to 5 and rack number 1 from position number 1 to sample number 5, and AST is also measured from sample number 6 to 10 in the same manner from rack number 1 to position number 2 to 5 times. When measuring once, input from the screen as follows. First, the sample type 301 is input, and then the sample number 302, the rack number 303, and the position number 304 are input. When the sample number-rack number-position number combination is shown in order, sample number 1 is 1-1-1, sample number 2 is 2-1-1, and 3-1-1, 4-1-1, 5- 1-1, 6-1-2, 7-1-2, 8-1-2, 9-1-2, and 10-1-2. Request all AST (aspartate aminotransferase) with the measurement request item selection key 305. Finally, the cup type 306 used for measurement is input. When the rack with the rack number 1 is inserted, the device positions the position number 1 of the rack with the rack number 1 in the sample dispensing area and places the sample probe in the position with the rack number 1 for AST measurement. Push 5 times into the number 1 sample container. At the interval between the fifth and sixth dispensing operations, the rack is moved and positioned by one sample in the traveling direction, and the AST dispensing operation is performed five times from position number 2 of rack number 1. Conventionally, when AST is dispensed once with one sample, the rack is forcibly moved by one sample, so ten sample containers are required. However, two sample containers placed in No. 1 and No. 2 require 10 sample containers. Test measurements can be performed, saving labor for experiments and saving sample containers.
[0022]
As shown in FIG. 4, the same measurement can be performed using a sample ID number 401 instead of the rack number 303 and the position number 304 in FIG.
[0023]
FIG. 5 shows an example in which AST is requested a plurality of times for the same item, for example, as described above.
[0024]
Here, the contents of the measurement request are shown as follows.
[0025]
AST: aspartate aminotransferase
ALT: Alanine aminotransferase
LD: Lactate dehydrogenase
TP: Total protein
ALP: alkaline phosphatase
GLU: Glicose
ALB: Alpmin
T-BIL: Total bilirubin
D-BIL: Direct bilirubin
GGT: γ-glutamyl transpeptidase
LAP: Leucine aminopeptidase
MG: Magnesium
IP: inorganic phosphorus
FE: Iron
CHE: Cholinesterase
T-CHO: Total cholesterol
F-CHO: Free cholesterol
BUN: urea nitrogen
PL: Phospholipid
HDL-C: HDL-cholesterol
TTT: Thymol turbidity test
ZTT: Zinc sulfate turbidity test
NA: Sodium
K: Potassium
CL: Chlorine
CU: Copper
CRP: C-reactive protein
RF: rheumatoid factor
[0026]
FIG. 6 shows a modification of the rack transport system of the automatic analyzer shown in this embodiment.
[0027]
In the rack transport system described above, the rack 7 is merely moved from the loading side to the storage side. However, in this example, the rack 7 can be moved from the loading side to the storage side and from the storage side to the loading side. The rack is sandwiched between the claws from both sides by the rack holder 16 connected to the conveyor belt 15 so as to be able to perform the reverse operation. According to the present embodiment, when measurement of two or more kinds of different concentration samples is necessary as in sample carryover measurement, the sample once used for measurement and returned to the sample dispensing position is returned to the sample dispensing position. Can be measured.
[0028]
FIG. 7 shows another modification of the rack transport method of the automatic analyzer shown in this embodiment.
[0029]
The operator sets the rack on which the sample is placed in the rack loading unit 1 and starts the apparatus. The first rack is measured, and the measured rack once sample dispensing has been completed is circulated using the retest line 11 for the retest sample possessed by the apparatus and measured again. Therefore, the same sample can be continuously measured.
[0030]
The rack 7 positioned in the sample dispensing area is dispensed according to the request information, returned to the main line 8 after the measurement, returned to the retest line 11 for the retest sample, and returned to the rack input unit 1 to return to the rack. After the rack number or sample ID is read again by the number or sample ID reader 10, it is positioned again in the sample dispensing area and dispensed. The rack for which all requested measurements are completed is stored in the rack storage unit 5. Since the same sample container can be used repeatedly for measurement, the labor of experiments is saved and the number of sample containers used is reduced.
[0031]
FIG. 8 shows a modification of the sample measuring method of the automatic analyzer shown in this embodiment. In this example, a continuous measurement sample container containing a large amount of sample, particularly a continuous measurement dedicated sample container 14 and a sample container holder 13 for setting this container are provided above the sample dispensing position of the apparatus. When a sample is set in or filled with the sample container holder 13 for the continuous measurement, the detection that the container has been set is entered, and the apparatus recognizes that the evaluation mode has been entered. When the start switch is pressed, the apparatus performs measurement according to the evaluation measurement request information input by the operator. Since it is not necessary to prepare any sample container, the labor of the experiment can be saved.
[0032]
Next, the basic performance measurement of the apparatus will be described.
[0033]
FIG. 9 shows a flowchart for measuring the simultaneous reproducibility.
[0034]
The simultaneous reproducibility measurement is one type of basic performance measurement, and is a test for checking the reproducibility (average value, standard deviation, coefficient of variation) of measured values when the number of samples is about 20 or more.
[0035]
The operator selects the measurement mode “reproducibility” which is one of the basic performance measurement items on the basic performance measurement screen of the apparatus (S901), the sample type (S902), the cup type (S903), and the number of measurements (N number). (S904), the number of samples (S905), and the measurement item (S906) are input in order. A rack on which the sample container is placed is prepared and set (S907), and the apparatus is started (S908). The apparatus reads the rack number and sample ID (S909), detects the presence or absence of the sample container (S910), and dispenses the sample (S911). The measurement ends with the output of the result (S912). When the reproducibility calculation key is pressed after the measurement is completed, the reproducibility result is automatically calculated and displayed (S913).
[0036]
FIG. 10 is an example of an input screen for measurement conditions for simultaneous reproducibility measurement. The operation will be specifically described with reference to this screen example.
[0037]
When measuring the AST reproducibility with the number of measurements (N number) of 20 and the number of samples of 2, the following is input from the screen. First, “Reproducibility” is selected with the selection key 1001. A sample type 1002 and a cup type 1003 are input. Next, when 20 is input to the number of measurements (N number) 1004 and 2 is input to the number of samples 1005, and the AST is requested with the measurement item selection key 1006, the apparatus automatically calculates and recognizes that a dispensing operation is performed 10 times from one sample. To do. The sample container is set in order from the position 1 of the rack, and the rack is installed on the apparatus. When the start key 1007 is pressed, the apparatus starts measurement. The sample probe thrusts 10 times into the sample container at position 1 of the rack that has been transported to the sample dispensing position for AST measurement. Thereafter, the rack moves in the traveling direction for one sample, and at position 2, a dispensing operation is performed 10 times for AST measurement. Thereafter, the rack is stored in the rack storage unit. When the reproducibility calculation key 1008 is pressed after the measurement is completed, the reproducibility result is automatically calculated and displayed. Conventionally, since the rack is forcibly moved by one sample when the dispensing operation of one sample is completed, 20 sample containers are required. However, if this method is used, 20 tests can be measured with two samples. As described above, the ability to measure the same item from the same sample container even if the sample number changes can save the labor of the experiment and reduce the waste such as the used sample container.
[0038]
FIG. 11 is an example of an input screen for measurement conditions for linearity measurement. The operation will be specifically described with reference to this screen example. The linearity test is a test for confirming to what extent the measured value of the apparatus has linearity as the concentration of the object to be measured gradually increases.
[0039]
First, the operator selects “linearity” using the selection key 1101 on the apparatus screen. Next, as measurement conditions, the number of series to be measured 1102, the concentration 1103a to j of each sample, the sample type 1104 of each sample, the sample number 1105, the rack number 1106, the position number 1107, the cup type 1108, and the measurement item 1109 are sequentially input. To do. Set the sample to be measured and press the start key 1110 of the apparatus. The apparatus reads the rack number and sample ID, detects the presence or absence of the sample container, and dispenses the sample. For the first sample, the rack position is fixed and the dispensing operation is performed until the dispensing operation for all requested items is completed. After the dispensing operation for this sample is completed, the device feeds the rack position for one sample. Continue dispensing until there is no unmeasured sample. The measurement ends with the output of the result. Data is output on the screen and printed out all measured values and calculated values for each series. By pressing the result confirmation key 1111, a linearity graph is displayed on the screen.
[0040]
As described above, the function of measuring the same item from the same sample container even if the sample number changes can save the labor of the experiment and reduce the waste such as the used sample container.
[0041]
FIG. 12 is a screen example of measurement condition input for the coexisting substance test. The input method and operation will be specifically described using this screen example. The coexisting substance test is used to measure the degree of influence of coexisting substances, and how much the substance that interferes with the reaction between the target substance and the reagent contained in the sample is measured against the original target substance. It is a test to confirm whether it affects the above. In general, typical substances that interfere with this reaction in laboratory test items include four components: intralipos (chyle component), ascorbic acid, hemoglobin (hemolytic component), and bilirubin (jaundice component). In the measurement, 11 kinds of samples prepared by mixing the coexisting substance and the sample stepwise (0/10 to 10/10) are analyzed, and the relationship between the added amount of the coexisting substance and the value of the target substance is compared. Check the degree of interference. Furthermore, a sample whose concentration is adjusted in several stages is measured a plurality of times, and the variation is confirmed.
[0042]
The operator selects “coexisting substances” using the selection key 1201 on the apparatus screen. Next, as the measurement conditions, the series number 1202 and the coexisting substance concentrations 1203a to j of each sample, the sample type 1204 of each sample, the sample number 1205, the rack number 1206, the position number 1207, the cup type 1208, and the measurement item input area, Measurement items 1209 are sequentially input. Set the rack on which the sample to be measured is placed, and press the start key 1210 of the apparatus. The apparatus reads the rack number and sample ID, detects the presence or absence of the sample container, and dispenses the sample. For the first sample, the rack position is fixed and the dispensing operation is performed until the dispensing operation for all requested items is completed. After the dispensing operation of this sample is completed, the rack position is fed by one sample. Continue dispensing until there is no unmeasured sample. The measurement ends with the output of the result. In data output, all measured values of each series are displayed on a screen and printed on a printer. By pressing the result confirmation key 1211, a graph of coexisting substances is displayed on the screen.
[0043]
When a measurement request is made on the screen of FIG. 12, the same rack number is input to the rack number input area and the same position number is input to the position number input area for the sample to be measured a plurality of times. Repeat measurement. Taking the measurement of the number of times of measurement of racks (N number) = 5 where 5 samples can be placed in one rack as an example, the sample numbers 1 to 5 are all 1 as the rack number and 1 as the position number in FIG. Select items such as AST, ALT, LD,.
[0044]
Subsequently, for the sample numbers 6 to 10, the rack number is 1 and the position number is 2, and the same items as above are selected.
[0045]
Hereinafter, sample numbers 11 to 15 are rack number 1, position number 3,
Sample numbers 16-20 are rack number 1, position number 4,
Sample numbers 21-25 are rack number 1, position number 5,
Sample numbers 26-30 are rack number 2, position number 1,
Sample numbers 31-35 are rack number 2, position number 2,
Sample numbers 36-40 are rack number 2, position number 3,
Sample numbers 41 to 45 are rack number 2, position number 4,
Sample numbers 46-50 are rack number 2, position number 5,
Sample Nos. 51 to 55 are selected with rack No. 3 and position No. 1, respectively, and after dispensing the sample whose concentration was adjusted in 11 stages into cups, the sample is set in the designated rack and the analysis is started. . As described above, the ability to measure the same item from the same sample container even if the sample number changes can save the labor of the experiment and reduce the waste such as the used sample container.
[0046]
The carry-over test of the stir bar is a measure of whether and how much the reagent components attached to the surface of the stir bar are carried over to the reaction solution for the next item measurement after washing, and how much the measurement value of the next item is affected. This is a test to check whether the value is given.
[0047]
For example, when measuring the carry-over of a stir bar from item A to item B, it has the same value to see how much the reagent components that adhere to the stir bar and carry over to the next test affect the next item The sample must be measured continuously.
[0048]
Normally, the operator measures the average value of the B item at a measurement count of about 30 under the condition that no carryover is applied, and further measures the B item after the A item in “AAA / BBB / AAA / BBB ...”. Repeat as shown. The measured value of the B item immediately after the A item is compared with the average value of the B item, and if there is a significant difference, it is determined that there is an influence.
[0049]
The operation of the apparatus when measuring the carryover of the stirring bar will be described with reference to the screen example of FIG. 13 and FIG.
[0050]
The operator selects “stirring bar carryover” with the selection key 1301 in FIG. 13. Next, the operator measures the sample type 1302, the cup type 1303 of the measurement conditions, the number of samples 1304 which means the number of sample containers used for the measurement, the number of times of measurement 1305 of the reference value measurement of the measurement item on the affected side, and the influential side The measurement item name 1306 and the number of consecutive tests 1307, the affected measurement item name 1308 and the number of consecutive tests 1309, and the number of measurement rounds 1310 for examining the influence from the A item to the B item are sequentially input. The device divides the total number of tests by the number of samples used for measurement, and automatically calculates and recognizes the number of dispensings from one sample container.
[0051]
The operator sets the rack 7 on which the quality control sample 6 of FIG. When the start key 1311 of the apparatus of FIG. 13 is pressed, the rack 7 reads the rack number or sample ID with the rack number or sample ID reader 10 and detects the presence or absence of the sample container on the rack, and then the main line 8 It is introduced into the advance analysis units 2 and 3 and positioned at the sample dispensing position. Until the position number changes, the sample lobe is dispensed from one sample container according to the request information B item × 30 times, AAABBB... Data output is printed on the printer at the same time that all measured values for each test are displayed on the screen. By pressing the result confirmation key 1312 shown in FIG. 13, the calculated value of the carryover rate is displayed on the screen and printed on the printer.
[0052]
As described above, the ability to measure the same item from the same sample container even if the sample number changes can save the labor of the experiment and reduce the waste such as the used sample container.
[0053]
The reaction container carry-over test affects the measured value when reagent components adhering to the inner surface of a reaction container repeatedly used for measurement of different items after washing are carried over to the reaction liquid of the next item. This is a test to check how much to give.
[0054]
For example, when measuring the carryover of a reaction vessel from item A to item B, the same is applied to see how much the reagent components that adhere to the inner surface of the reaction vessel and carry over to the next test affect the measured value of the next item. Samples with values must be measured continuously.
[0055]
Usually, the operator first requests reproducibility measurement 30 times or more for measuring the reference value of the B item, and requests the measurement of the A item for about 10 tests in order to contaminate the reaction container with the reagent of the A item. In order to measure the B item in the reaction vessel group that measured the A item in the next round and see the carryover, the first A item measurement group so that the B item comes to the first reaction vessel of the A item reaction vessel group. The dummy item is requested from the test after the last test until the test immediately before the B item measurement that receives the carry-over of the next round. When the measured value of the B item measured in the reaction container in which the A item is measured is compared with the reference value (average value) of the B item having about 30 measurements, it is determined that there is an influence when there is a significant difference.
[0056]
An example of the operation of the apparatus when measuring the carryover of the reaction vessel will be described with reference to the screen example of FIG. 14 and FIG.
[0057]
The operator selects “reaction vessel carryover” using the selection key 1401 on the screen of FIG. Next, the operator measures the sample type 1402 of the measurement condition, the cup type 1403, the number of samples 1404 which means the number of sample containers used for the measurement, the number of measurements 1405 of the reference value measurement of the measurement item on the affected side, and the influential side The measurement item name 1406 and its consecutive test number 1407, the affected measurement item name 1408 and its continuous test number 1409, and the dummy item item name 1410 for filling the interval are sequentially input. The device divides the total number of tests by the number of samples used for measurement, and automatically calculates and recognizes the number of dispensings from one sample container.
[0058]
The operator sets the rack 7 on which the quality control sample 6 of FIG. When the start key 1411 of FIG. 14 is pressed, the rack 7 reads the rack number or sample ID with the rack number or sample ID reader 10 and detects the presence or absence of the sample container on the rack, and then proceeds to the main line 8 for analysis. It is introduced into the parts 2 and 3 and positioned at the sample dispensing position. Until the position number on the rack changes, the sample lobe starts from one sample container in the order of B item group, A item group, dummy item group, B item group, 30 tests for B item, 10 tests for A item, In order to fill the reaction container from the test following the last test of the A item group to the second test immediately before the B item group, sample dispensing was performed in the order of the dummy item and the B item 10 test affected by the A item. Do. For data output, all measured values for each test are displayed on the screen and printed on a printer. By pressing the result confirmation key 1412, the calculated carryover value is displayed on the screen and printed on the printer.
[0059]
As described above, the ability to measure the same item from the same sample container even if the sample number changes can save the labor of the experiment and reduce the waste such as the used sample container.
[0060]
As described above, in the automatic analysis method that measures multiple samples one after another by sample dispensing and reagent dispensing, the measurement sample item, requested measurement item, and basic performance measurement item are displayed on the screen, and the same rack When dispensing the sample at the same position multiple times, the contents of the measurement sample and requested measurement are indicated on the screen, this is memorized, the screen is changed, and the next basic performance measurement from the basic performance measurement items, that is,
Reproducibility measurement of measured values,
Linearity measurement of measured values,
Coexistence substance influence measurement,
Carryover measurement of a stirring bar,
Carryover measurement of reaction vessel,
Carryover measurement of reagent probe,
Sample probe carry-over measurement
There is provided an automatic analysis method for instructing one of the measurements, setting the rack at the same position, and dispensing the sample at the same position.
[0061]
The reagent probe carry-over test measures whether and how much of the reagent component adhering to the inner and outer surfaces of the reagent probe is carried over to the reaction liquid of the next item after washing, and affects the measured value of the next item. This is a test to check whether the value is given. In this case, the carry-over test of the reagent probe usually narrows down items to be received and items to be given in the screening test, and carries out a carry-over confirmation test. In the embodiment of the present invention, a carry-over confirmation test will be described.
[0062]
For example, when measuring the carry-over of a reagent probe from item A to item B, the same applies to see how much the reagent components that adhere to the inner and outer surfaces of the reagent probe and carry over to the next test affect the next item. Samples with values must be measured continuously.
[0063]
Usually, the operator measures the average value of the B item under the condition of not carrying over at the number of measurement of about 30, and further measures the B item after the A item in “AAA / BBB / AAA / BBB. Repeat as shown. The measured value of the B item immediately after the A item is compared with the average value of the B item, and if there is a significant difference, it is determined that there is an influence.
[0064]
An example of the operation of the apparatus when measuring the carryover of the reagent probe will be described with reference to the screen example of FIG. 15 and FIG.
[0065]
The operator selects “reagent probe carryover” using the carryover R and probe selection key 1501 on the screen of FIG. Next, the operator affects the sample type 1502, the cup type 1503 of the measurement conditions, the number of samples 1504 which means the number of sample containers used for the measurement, the number of measurements 1505 when measuring the reference value of the measurement item on the affected side, and the influence. The measurement item name 1506 on the side and the number of consecutive tests 1507, the measurement item name 1508 on the affected side and the number of consecutive tests 1509, and the number of rounds 1510 for examining the influence from the A item to the B item are sequentially input. The device divides the total number of tests by the number of samples used for measurement, and automatically calculates and recognizes the number of dispensings from one sample container.
[0066]
The operator sets the rack 7 on which the quality control sample 6 of FIG. When the start key 1511 in FIG. 15 is pressed, the rack 7 reads the rack number or sample ID with the rack number or sample ID reader 10 and detects the presence or absence of the sample container on the rack, and then proceeds to the main line 8 for analysis. It is introduced into the parts 2 and 3 and positioned at the sample dispensing position. Until the position number on the rack changes, the sample lobe dispenses samples from one sample container according to the request information, B item × 30 times, AAABBB. For data output, all measured values for each test are displayed on the screen and printed on a printer. When the result confirmation key 1512 in FIG. 15 is pressed, the calculated carryover rate is displayed on the screen and printed on the printer.
[0067]
As described above, the ability to measure the same item from the same sample container even if the sample number changes can save the labor of the experiment and reduce the waste such as the used sample container.
[0068]
In the carry-over test of the sample probe, the sample components remaining on the inner and outer surfaces of the sample probe after washing are carried over to the reaction solution of the measurement item of the next sample and affect the measurement value of the next item. This is a test to check how much to give.
[0069]
Usually, an operator measures a high-concentration sample with a high concentration of the target substance for measurement three times in succession, and then continuously measures a low-concentration sample with zero measurement value without the target substance for measurement three times in succession. . A low-concentration sample with a measurement value of zero is measured in advance at about 30 measurements, and the average value (reference value) is compared with the measurement value of the low-concentration sample immediately after the high-concentration sample is measured. If there is a difference, it is judged that there is an influence. The carryover rate of the sample probe is obtained by dividing the difference between the reference value and the measured value of the low concentration sample immediately after measuring the high concentration sample by the concentration of the high concentration sample. The concentration of a high-concentration sample is usually measured as it is because it cannot be measured as it is.
[0070]
An example of the operation of the apparatus when measuring the carryover of the sample probe will be described with reference to the screen example of FIG. 16 and FIG.
[0071]
The operator selects “sample probe carry-over” with the select key 1601 indicating the carry-over S and probe on the screen of FIG. Next, the operator sequentially inputs a sample type 1602, a cup type 1603, and a measurement item 1604 to be measured for carryover as measurement conditions. Furthermore, rack number 1605 in which a high concentration sample is set, position number 1606, the number of times of dispensing 1607, rack number 1608 in which a low-concentration sample to be carried over is set, position number 1609, number of times of dispensing 1610, and concentration of the high concentration sample The rack number 1611, the position number 1612, and the number of times of dispensing 1613 in which the diluted sample diluted for measurement is set are sequentially input.
[0072]
Take a 5 sample rack as an example. The operator sets the high-concentration sample (hereinafter referred to as Sample A) to the rack number, 1 to the position number, 1 to the number of dispensings, 3 to the rack number where the low-concentration sample physiological saline (hereinafter referred to as Sample B) is set to 1, Position number 2, 3, 4; 1 for each number of dispenses; 1 for diluted sample (hereinafter sample C); 2 for rack number; 1, 2, 3, 4, 5 for position number; 1 for each number of dispenses Are sequentially input, and the sample and the rack of rack number 1 and rack number 2 are set in the rack loading unit 1 according to the input information. When the start key 1614 is pressed, the rack 7 reads the rack number or sample ID with the rack number or sample ID reader 10 and detects the presence or absence of the sample container on the rack. , 3 and positioned at the sample dispensing position. Sample A at position number 1 in the first rack is dispensed three times in succession, and sample B at subsequent position numbers 2, 3, and 4 is dispensed once at each sample container. Further, the sample C of position number 1, 2, 3, 4, 5 of rack number 2 is dispensed once for each sample container, a total of 11 times, and the measurement is completed. For data output, all measured values for each test are displayed on the screen and printed on a printer. By pressing the result confirmation key 1615, the calculated carryover rate is displayed on the screen and printed on the printer.
[0073]
As described above, the ability to measure the same item from the same sample container even if the sample number changes can save the labor of the experiment and reduce the waste such as the used sample container.
[0074]
【The invention's effect】
The present invention has the following effects.
[0075]
In the present invention, a screen having measurement items and basic performance measurement items is added to specify the number of times of measurement of the same item for a sample at the same position, and in this case, the rack is stopped at the same position, so that the automatic analyzer Measurement of basic performance of devices such as simultaneous reproducibility, linearity of measured values, influence of coexisting substances, confirmation of presence / absence of carryover of sample probe / reagent probe / stirring rod / reaction vessel Can also be easily done. In addition, the efficiency of work is improved in the sense that the operator can leave the apparatus with peace of mind. Since the number of sample containers to be handled can be reduced, the work time can be shortened and work errors can be reduced.
[0076]
Furthermore, by reducing the number of sample containers to be used and the amount of sample consumption, the waste of used sample containers and samples themselves that are at risk of infection can be reduced, contributing to environmental problems.
[Brief description of the drawings]
FIG. 1 is an external view of an automatic analyzer having a function of repeatedly measuring the same sample.
FIG. 2 is a flowchart for repeatedly measuring the same sample.
FIG. 3 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 4 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 5 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 6 is an external view of an automatic analyzer having a function of repeatedly measuring the same sample.
FIG. 7 is an external view of an automatic analyzer having a function of repeatedly measuring the same sample.
FIG. 8 is an external view of an automatic analyzer having a function for repeatedly measuring the same sample.
FIG. 9 is a flowchart for repeatedly measuring the same sample.
FIG. 10 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 11 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 12 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 13 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 14 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 15 is a diagram showing an example of a measurement request screen of the automatic analyzer.
FIG. 16 is a diagram showing an example of a measurement request screen of the automatic analyzer.
[Explanation of symbols]
DESCRIPTION OF SYMBOLS 1 ... Rack input part, 2 ... Analysis part D, 3 ... Analysis part P, 4 ... Conveyance line, 5 ... Rack storage part, 6 ... Sample container, 7 ... Rack, 8 ... Main line, 9 ... Retest buffer, 10 ... Rack number or sample ID reader, 11 ... retest line, 12 ... re-measurement rack, 13 ... sample container holder, 14 ... sample container for repeated measurement, 15 ... conveying belt, 16 ... rack holder.

Claims (4)

A rack on which a plurality of sample containers can be installed;
An analysis unit for analyzing a sample in a sample container installed on the rack;
A transport line for transporting the analysis unit to the rack;
A loading section for loading the rack into the transport line;
A storage unit for storing racks that have been analyzed;
In an automatic analyzer equipped with
In the same sample container for analysis of general samples and the evaluation mode that can be specified so that sorting and discharging can be repeated multiple times for the samples placed in the same sample container built in the rack An automatic analyzer comprising a display device for displaying a screen for switching a measurement mode in which sorting and discharging cannot be repeated a plurality of times for a sample placed therein.   The automatic analyzer according to claim 1, further comprising: a sample container for continuous measurement for repeating sorting and discharging a plurality of times, and a rack including a sample container holder for setting the sample container for continuous measurement, and the sample container for continuous measurement An automatic analyzer having a function of automatically setting an evaluation mode when a sample is set in   3. The automatic analyzer according to claim 1, wherein when switching to the evaluation mode, the screen comprises reproducibility, linearity, coexisting substances, carryover stirring bar, carryover reaction vessel, carryover reagent probe, and carryover sample probe measurement. An automatic analyzer having a function of displaying any item of a basic performance measurement item group and executing the displayed basic performance measurement item in response to selection of any item. The automatic analyzer according to any one of claims 1 to 3, further comprising a retest line that circulates the measured rack once sample dispensing has been completed so as to be measured again.

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