JP2011195534A - Hyaluronic acid production promoter, anti-ageing agent and wrinkle-ameliorating agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a hyaluronic acid production promoter, an anti-ageing agent and a wrinkle-ameliorating agent each exhibiting an excellent hyaluronic acid production-promoting action.SOLUTION: The hyaluronic acid production promoter, the anti-ageing agent and the wrinkle-ameliorating agent each comprise a plant body or an extract thereof of Kalopanax septemlobus (Thunb.) Koidz., Staphylea bumalda DC., Clerodendrum trichotomum Thunb. and Cardamine scutata Thunb.

Description

  The present invention relates to a hyaluronic acid production promoter, anti-aging agent and wrinkle improving agent having hyaluronic acid production promoting action.

  In recent years, research on aging has been promoted. As for the cause of skin aging, aging is an important factor when viewed macroscopically, but in addition to that, the effects of drying, oxidation, sunlight (ultraviolet rays) and the like are also directly related to skin aging. As specific phenomena of skin aging, reduction of mucopolysaccharides such as hyaluronic acid, cross-linking reaction of collagen, cell damage due to ultraviolet rays, and the like are known.

  Among them, hyaluronic acid retains moisture in the cell gap, retains cells based on the formation of a jelly-like matrix in the tissue, retains the lubricity and flexibility of the tissue, and resists external forces such as mechanical failure. And has many functions such as prevention of bacterial infection (see Non-Patent Document 1). For example, it is said that the amount of hyaluronic acid in the skin decreases with aging, and along with this, skin aging such as fine lines and roughness appears. For this reason, many cosmetics containing hyaluronic acid and collagen have been proposed as an ameliorating agent for such aging skin. However, these conventional cosmetics only exhibit a moisturizing effect on the skin surface, and cannot essentially improve aging skin. In addition, cosmetics containing various vitamins and herbal medicines as skin cell activators have been proposed, but these have not yet been able to improve and treat aging skin.

  Furthermore, the hyaluronic acid contained in the joint fluid covers the surface of the articular cartilage and is useful for smooth operation of the joint function. Hyaluronic acid concentration in normal human joint fluid is about 2.3 mg / ml, but in the case of rheumatoid arthritis, hyaluronic acid concentration in joint fluid is reduced to about 1.2 mg / ml and at the same time the viscosity of joint fluid is Remarkably reduced (see Non-Patent Document 2). In addition, it is known that hyaluronic acid content decreases in pyogenic arthritis and gouty arthritis as in the case of rheumatoid arthritis (see Non-Patent Document 3). In the above diseases, it is conceivable to increase the amount of hyaluronic acid in synovial fluid in order to improve lubrication function, cover / protect articular cartilage, suppress pain, and improve or normalize pathological joint fluid. For example, it has been reported that the above-mentioned improvement is observed when sodium hyaluronate joint injection therapy is performed on patients with rheumatoid arthritis (see Non-Patent Document 4). Similarly, in traumatic arthritis, osteoarthritis and osteoarthritis, the above-mentioned improvement effect has been reported by joint injection therapy with hyaluronic acid. (Refer nonpatent literature 5).

  In this way, cosmetics containing hyaluronic acid are applied to the skin, and hyaluronic acid is directly injected into the joint. It is not an improvement and a sufficient effect cannot be expected. In particular, there is a problem that hyaluronic acid is hardly absorbed from the skin.

  For this reason, instead of simply replenishing hyaluronic acid itself from the outside, by utilizing the self-healing power of the living body inherently possessed by humans, by promoting the hyaluronic acid producing ability of human skin fibroblasts themselves, Development of substances that fundamentally improve the functions of living organisms is expected, and among them, various plant-derived hyaluronic acid production promoters have been developed with the expectation of safety and mildness of skin irritation. (For example, see Patent Documents 1 to 3).

  In the healing process after burn injury, hyaluronic acid is known to increase significantly in the granulation during the period from the beginning of the granulation tissue growing from below the necrotic tissue until the entire tissue is replaced with granulation tissue. (See Non-Patent Document 6), hyaluronic acid production promoters are also expected as an early treatment for burns.

Japanese Patent Laid-Open No. 11-209261 JP 2007-84448 A JP 2003-55244 A

"Bio Industry", vol.8, p.346 (1991) "Arthritis Rheumatism", vol.10, p.357 (1967) "Combined composition" (Kanehara Publishing), 481, 1984 "Inflammation" (Japan Inflammation Society), 11, 16, 1991 "Connective tissue and diseases" (Kodansha), 246, 1980 "Connective tissue and diseases" (Kodansha), 153, 1980

As described above, various plant-derived extracts have been developed as having hyaluronic acid production promoting action, with the expectation of their safety and mild irritation to the skin. There is a need for further development of a new plant that has not been found to exhibit a new hyaluronic acid production promoting effect.
The present invention has been made in view of such conventional circumstances, and an object thereof is to provide a new plant-derived hyaluronic acid production promoter.

  In view of the current situation, the present inventors have conducted extensive research, and as a result, specific plant extracts have an excellent hyaluronic acid production promoting action and are useful as hyaluronic acid production promoters. The present inventors have found that the extract is useful as an anti-aging agent and a wrinkle improving agent based on hyaluronic acid production promoting action, and completed the present invention.

  The present invention is a hyaluronic acid production promoter characterized by containing one or more selected from plants, or extracts thereof, of barbs, honeybees, wedges, and seedlings.

  Moreover, this invention is the anti-aging agent characterized by including the 1 type (s) or 2 or more types chosen from the plant body of a honey-beetle or a seedling or a extract thereof.

  Furthermore, this invention is a wrinkle improving agent characterized by including the 1 type (s) or 2 or more types chosen from the plant body or the extract of a honey-beetle or a seedling.

  The hyaluronic acid production promoter of the present invention has an excellent hyaluronic acid production promoting action, preventing human skin aging, especially prevention and treatment of arthritis, etc., including skin agglomeration and elasticity retention, initial treatment of burns It can be effectively applied to the above. In addition, since ingredients derived from natural products are used, it is safe even for internal use or external application to humans or animals.

  The anti-aging agent and wrinkle-improving agent of the present invention promotes the production of hyaluronic acid, which is one of the extracellular matrix components, maintains the skin's elasticity and elasticity, prevents wrinkles, and keeps youthful skin with moisture. The effect is that the state can be maintained. In addition, since ingredients derived from natural products are used, it is safe even for internal use or external application to humans or animals.

Hereinafter, the present invention will be described in detail.
The hyaluronic acid production promoter, anti-aging agent and wrinkle improving agent of the present invention have excellent hyaluronic acid production promoting ability. Therefore, the hyaluronic acid production promoter, anti-aging agent and wrinkle improving agent of the present invention are suitable as materials for pharmaceuticals (for example, external preparations for skin and internal medicines), quasi drugs, and cosmetics. Moreover, it is applicable also to food-drinks (for example, a supplement and a drink).

The bark (scientific name: Kalopanax septemlobus (Thunb.) Koidz.) Used in the present invention is a plant belonging to the genus Harigiri.
Karosaponin in the bark is said to have a stimulating expectorant action and has been used as an expectorant in the private sector for a long time. It is also used for hemorrhoidal bleeding, bruises, and rheumatic pain ("Primary Color Makino Wakahan Herbal Encyclopedia"). Bark and bark of bark are used as a medicine for lower back and knee pain, bruise, hemorrhoids, and expectoration by Chinese medical doctors (“Worldly useful plant encyclopedia”). Japanese Patent Application Laid-Open No. 8-283172 describes an active oxygen scavenger containing a sharp edge and a skin external preparation containing the same.

  The honey beetle used in the present invention (scientific name: Staphylea bumalda DC.) Is a plant belonging to the genus honey beetle family, and is considered to improve dry cough and blood sterilization in women after birth ("Chinese medicine dictionary").

The peony (scientific name: Clerodendrum trichotomum Thunb.) Used in the present invention is a plant belonging to the genus Lepidoptera.
It has been reported that the stalks and leaves of magpie have antihypertensive and sedative effects. In addition, it is used for rheumatism, half body insufficiency, hypertension, product, etc., and the root is used for diuresis, healthy stomach, antipyretic, etc. (“Makino Kazuhan Herb Encyclopedia”). In Chinese medicine, branches and leaves are known to improve hypertension, migraine, malaria, diarrhea, epilepsy, etc. (“World Useful Plant Encyclopedia”). JP 2002-179581 discloses a skin aging inhibitor (skin aging inhibitor having an action of removing active oxygen) and JP 2000-178198 discloses a cell activator (aging prevention by fibroblast activation). Is used as an external preparation for skin having whitening, rough skin improvement, skin firmness and wrinkle improvement effects, and as an elastase inhibitor in JP 2002-179581 A.

The rapeseed (Scientific name: Cardamine scutata Thunb.) Used in the present invention is a plant belonging to the genus Brassicaceae.
It is known that Tanetsukibana is used for swelling, swelling, cough, asthma attack, urethritis, cystitis, squeaks, artifacts, etc. ("Primary Color Makino Japanese Herbal University Encyclopedia").

Thus, there has never been a report that the plant body or plant extract of each plant described above has a hyaluronic acid production promoting action, which has been found for the first time by the present inventors.
In addition, as for Mitsubatsugi and Seedlings, it is not known that the plants or plant extracts of these plants have an anti-aging effect and a wrinkle-improving effect.

  In addition, the plant body of each plant used in the present invention or an extract thereof refers to various parts of each plant body (flower, flower ear, fruit skin, fruit, stem, leaf, branch, branch leaf, trunk, bark, rhizome, root). Skin, roots, seeds, whole grass, etc.) as they are or dried and pulverized into dry powders, or as they are or after drying and pulverizing and then extracted with a solvent.

  In the case of an extract, the extraction solvent used for extraction may be any solvent that is usually used for extraction, in particular alcohols such as methanol, ethanol or 1,3-butylene glycol, hydrous alcohols, acetone, ethyl acetate, etc. These organic solvents can be used alone or in combination. Of these, alcohols and hydrous alcohols are particularly preferred, and methanol, ethanol, 1,3-butylene glycol, hydrous ethanol or hydrous 1,3-butylene glycol are particularly preferred. . The solvent is preferably used at a temperature between room temperature and the boiling point of the solvent.

  There are no particular limitations on the extraction method, but usually it may be in the range of the boiling point of the solvent at room temperature to normal pressure. After extraction, the solution is filtered, ion exchanged, adsorbed, decolored and purified. , Paste, gel, and powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decoloration may be added as long as the effect is not affected. An activated carbon column or the like may be used, and usual means generally applied depending on the extracted substance may be arbitrarily selected.

  As the extraction part of the plant body, leaves and branches are preferable for sharpening, branches are for honeybees, leaves are for peony, and whole plants are for spinach, but extracts from other parts can also be used.

  The hyaluronic acid production promoter, anti-aging agent, and wrinkle-improving agent of the present invention are preferably composed of one or more of the aforementioned plants or extracts thereof, but do not impair the effects of the present invention. In addition, various other components can be contained.

  The hyaluronic acid production promoter, anti-aging agent, and wrinkle-improving agent of the present invention can be used in humans and animals by blending with external preparations for skin, as well as blended with various foods and drinks, feeds (such as pet food) Can be ingested. It can also be administered to humans and animals as a pharmaceutical formulation. At this time, it may be adjusted by arbitrarily drying, concentrating or diluting depending on the dosage form and form of the external preparation for skin and food and drink.

  When the hyaluronic acid production promoter, anti-aging agent, and wrinkle improving agent of the present invention are blended in a skin external preparation, an extract of each plant is preferable, and the blending amount (dry mass) is generally 0 in the total amount of the external preparation. 0.0001-10 mass% is preferable.

  When the present invention is applied to an external preparation for skin, in addition to the above components, components that are usually used in external preparations for skin such as cosmetics and pharmaceuticals within the range that does not impair the effects of the present invention, if necessary, such as antioxidants Oils, UV protection agents, surfactants, thickeners, alcohols, powder components, color materials, aqueous components, water, various skin nutrients, and the like can be appropriately blended as necessary.

  Furthermore, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, sequestering agents such as gluconic acid, preservatives such as methylparaben, ethylparaben, butylparaben, caffeine, tannin, Verapamil, tranexamic acid and its derivatives, licorice extract, grabrizine, hot water extract of karin fruit, various herbal medicines, tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, Whitening agents such as ascorbic acid glucoside, arbutin, and kojic acid, and sugars such as glucose, fructose, mannose, sucrose, and trehalose can also be appropriately blended.

  The external preparation for skin can be applied to cosmetics, quasi-drugs, etc., particularly preferably cosmetics applied to the outer skin, and the dosage form can also be applied to the skin. Any dosage form such as solution system, solubilization system, emulsification system, powder dispersion system, water-oil two-layer system, water-oil-powder three-layer system, ointment, lotion, gel, aerosol, etc. is applicable. Is done.

  The use form is also arbitrary, and for example, it can be used for facial cosmetics such as lotions, emulsions, creams and packs, makeup cosmetics such as foundations, lipsticks and eye shadows, aromatic cosmetics, bath preparations and the like.

  In addition, makeup cosmetics can be widely applied to foundations and the like, and toiletries such as body soaps and soaps. Furthermore, if it is a quasi-drug, it can be widely applied to various ointment forms. And the form which can take the hyaluronic acid production promoter of this invention, an anti-aging agent, and a wrinkle improving agent is not limited to these dosage forms and forms.

  When the hyaluronic acid production promoter, anti-aging agent, and wrinkle improving agent of the present invention are blended in foods and drinks, feeds, etc., the blending amount (dry mass) of the plant body or its extract is the type, purpose, and form thereof. Depending on the method of use and the like, it can be appropriately determined. For example, in the case of tablets and capsules in the total amount of food and drink, 1 to 90% by weight is preferable, and in other foods and drinks, 0.001 to 50% by weight is preferable. preferable. It is preferable to prepare so that the daily intake of the plant or its extract per adult is about 1 to 1,000 mg. In particular, when used as a health food or drink, it is preferable to contain the active ingredient of the present invention in such an amount that a predetermined effect is sufficiently exhibited.

  As a form of food-drinks or feed, it can be arbitrarily shaped into, for example, granules, granules, pastes, gels, solids, or liquids. These include various known substances that are recognized to be contained in foods and drinks, such as binders, disintegrants, thickeners, dispersants, reabsorption accelerators, flavoring agents, buffers, and surfactants. Excipients such as solubilizers, preservatives, emulsifiers, tonicity agents, stabilizers and pH adjusters may be included as appropriate.

  When the hyaluronic acid production promoter, anti-aging agent and wrinkle improving agent of the present invention are used as pharmaceutical preparations, the preparations are appropriately used orally or parenterally (intravenous administration, intraperitoneal administration, etc.). . The dosage form is also arbitrary, for example, oral solid preparations such as tablets, granules, powders and capsules, oral liquid preparations such as internal liquids and syrups, and parenteral liquid preparations such as injections. These forms can also be appropriately prepared by known methods. For these pharmaceutical preparations, commonly used binders, disintegrants, thickeners, dispersants, reabsorption accelerators, corrigents, buffers, surfactants, solubilizers, preservatives, emulsifiers, isotonicity Excipients such as agents, stabilizers and pH adjusters may be used as appropriate.

When the hyaluronic acid production promoter, anti-aging agent and wrinkle-improving agent of the present invention are used as an external preparation for skin, foods and drinks, pharmaceutical preparations, etc., it has an excellent hyaluronic acid production promoting action, thereby preventing aging of human skin, It is effectively used for beam and elasticity retention, prevention and treatment of arthritis, etc., and early treatment of burns. When added to a food, it is expected that the hyaluronic acid production promoting effect will be exhibited from the inside by ingesting the food.
In addition, with the concept of physiological functions such as treatment, prevention, and improvement of the above symptoms and pathological conditions, it can be applied to external preparations for skin, functional foods and beverages, foods for specified health use, and the like.

  The method for adding the hyaluronic acid production promoter, anti-aging agent and wrinkle-improving agent of the present invention to an external preparation for skin or food and drink may be added in advance or during production. It may be selected as appropriate in consideration of workability.

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. Unless otherwise specified, the amount is shown in mass%.
Initially, the preparation method of the plant extract used in the present Example, the test method regarding the hyaluronic acid production promotion effect, and the result are demonstrated.

1. Sample preparation All samples were grown in Kochi Prefecture and obtained from the Kochi Prefecture Makino Memorial Foundation.
The following samples were prepared and used as test samples.
(1) Harigiri leaf extract 8.18 g of dried Harigiri leaf was immersed in 80 mL of methanol at room temperature for 1 week. Subsequently, the solvent was distilled off from the extract obtained by filtration with filter paper to obtain 1228 mg (yield: 15.0%) of barley leaf methanol extract.
(2) Bee extract Subsequently, the solvent was distilled off from the extract obtained by filtration with a filter paper to obtain 323 mg (yield: 3.6%) of a Mitsubatsugi methanol extract.
(3) Wedge Extract 7.38 g of dried Wedge leaves were immersed in 80 mL of methanol for 1 week at room temperature. Subsequently, the solvent was distilled off from the extract obtained by filtration with filter paper to obtain 909 mg (yield: 12.3%) of Kusagi methanol extract.
(4) Seedling Bana Extract 8.07 g of the dried Seedlings of whole plant was immersed in 80 mL of methanol at room temperature for 1 week. Subsequently, the solvent was distilled off from the extract obtained by filtration with a filter paper to obtain 1488 mg (yield: 18.4%) of the seed extract of methanol.

2. Hyaluronic acid production promoting effect test method and results 1. Using each plant extract obtained in the above as a test sample, the hyaluronic acid production promoting effect was measured and evaluated by the following method.

(1) Test method of hyaluronic acid production promoting action The hyaluronic acid production promoting action of each sample was evaluated. That is, human dermal fibroblasts were seeded in a 24-well plate at a concentration of 5.0 × 10 4 cells / well and brought to a confluent state. .5% FBS) medium was added. After the addition, the mixture was incubated for 72 hours, and the amount of hyaluronic acid produced was measured by ELISA. A DMSO / PBS solution (no sample added) was used as a control example (control). Hyaluronan assay kit (manufactured by Seikagaku Biobusiness) was used for the measurement.
The results are shown in Table 1, with the relative value of hyaluronan (hyaluronan, synonymous with hyaluronic acid) calculated with respect to 100 as the control.

As can be seen from Table 1, the plant extract of the present invention has an excellent hyaluronic acid production promoting effect and is useful as a hyaluronic acid production promoter and anti-aging agent.
Therefore, by incorporating the hyaluronic acid production promoter of the present invention, it is possible to provide an external preparation for skin having an effect of promoting hyaluronic acid production, an oral composition (for example, a functional agent, food and drink), a pharmaceutical preparation and the like. It is useful for prevention, prevention, improvement, treatment, etc. of various symptoms, diseases, and pathological conditions.
Specific examples of application include prevention, improvement and treatment of skin aging such as sagging / wrinkling of skin, reduction of elasticity and elasticity, prevention / treatment of arthritis, and early treatment of burns. However, application is not limited to these examples.

  Below, the formulation example of the hyaluronic acid production promoter by this invention of various dosage forms is demonstrated as a formulation example. Needless to say, the present invention is not limited by these formulation examples and is specified by the scope of claims.

[Formulation Example 1: Candy]
2000mg sugar
Minamata 1926mg
Hyaluronic acid production promoter: hot water extract (dry mass) 20mg
Collagen peptide 10mg
Pearl barley extract (dry mass) 2mg
Lotus germ extract (dry mass) 2mg
Fragrance 40mg
(Total) 4000mg

[Formulation Example 2: Tablet]
Sucrose ester 70mg
Crystalline cellulose 74mg
Methylcellulose 36mg
Glycerin 25mg
Black bear extract 300mg
Hyaluronic acid production promoter: Honey beetle hydrous ethanol extract (dry mass) 120mg
Collagen peptide 60mg
Pearl barley extract (dry mass) 10mg
N-acetylglucosamine 180mg
Hyaluronic acid 150mg
Vitamin E 30mg
Vitamin B6 20mg
Vitamin B2 10mg
α-Lipoic acid 20mg
Coenzyme Q10 40mg
Ceramide (konjac extract) 55mg
L-proline 300mg
(Total) 1500mg

[Formulation formulation example 3: soft capsule A]
Edible soybean oil 528mg
Hyaluronic acid production promoter: Wedge water-containing ethanol extract (dry mass) 90 mg
Collagen peptide 45mg
Pearl extract (dry mass) 8mg
Lotus germ extract (dry mass) 16mg
Royal Jelly 70mg
Maca 60mg
GABA (γ-aminobutyric acid) 30mg
Beeswax 60mg
Gelatin 375mg
Glycerin 113mg
Glycerin fatty acid ester 105mg
(Total) 1500mg

[Formulation Example 4: Soft Capsule B]
Brown rice germ oil 650mg
Hyaluronic acid production promoter: hot spring water extract (dry mass) 300mg
Collagen peptide 255mg
Pearl barley extract (dry mass) 50mg
Resveratrol 5mg
Elastin 180mg
DNA 30mg
Folic acid 30mg
(Total) 1500mg

[Formulation Example 5: Granules]
Hyaluronic acid production promoter: Barely hydrous ethanol extract (dry mass) 140mg
Collagen peptide 70mg
Pearl barley extract (dry mass) 20mg
Lotus germ extract (dry mass) 40mg
Vitamin C 150mg
Soy isoflavone 270mg
Reduced lactose 360mg
Soybean oligosaccharide 36mg
Erythritol 36mg
Dextrin 30mg
Fragrance 24mg
Citric acid 24mg
(Total) 1200mg

[Composition Example 6: Drink (in 50 mL)]
Hyaluronic acid production promoter: Honey beetle hot water extract (dry mass) 10mg
Collagen peptide 5mg
Pearl barley extract (dry mass) 2mg
Reduced maltose starch syrup 28mg
Erythritol 8mg
Citric acid 2mg
Fragrance 1.3mg
N-acetylglucosamine 1mg
Hyaluronic acid 0.5mg
Vitamin E 0.3mg
α-Lipoic acid 0.2mg
Coenzyme Q10 1.2mg
Ceramide (konjac extract) 0.4mg
L-proline 2mg
Water residue

[Formulation Example 7: Ointment]
(Compounding ingredients) (mass%)
Hyaluronic acid production promoter: Kusagi methanol extract (dry mass) 1.0
Stearyl alcohol 18.0
Owl 20.0
Polyoxyethylene (20) monooleate 0.25
Glycerin monostearate 0.3
Vaseline 40.0
Purified water residue

[Formulation formulation example 8: serum]
(Compounding ingredients) (mass%)
(Phase A)
95% ethyl alcohol 10.0
Polyoxyethylene (20) octyldodecanol 1.0
Pantothenyl ethyl ether 0.1
Hyaluronic acid production promoter: water extract of water containing edible crabs (dry mass) 1.5
Methylparaben 0.15
(Phase B)
Potassium hydroxide 0.1
(Phase C)
Glycerin 5.0
Dipropylene glycol 10.0
Sodium bisulfite 0.03
Carboxyvinyl polymer 0.2
Purified water residue

[Composition example 9: Pack]
(Compounding ingredients) (mass%)
(Phase A)
Dipropylene glycol 5.0
Polyoxyethylene (60) hydrogenated castor oil 5.0
(Phase B)
Hyaluronic acid production promoter: Harimiri methanol extract (dry mass) 0.01
Olive oil 5.0
Tocopherol acetate 0.2
Ethylparaben 0.2
Fragrance 0.2
(Phase C)
Sodium bisulfite 0.03
Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
Ethanol 7.0
Purified water residue

[Formulation formulation example 10: emulsion]
(Compounding ingredients) (mass%)
Microcrystalline wax 1.0
Beeswax 2.0
Lanolin 20.0
Liquid paraffin 10.0
Squalane 5.0
Sorbitan sesquioleate ester 4.0
Polyoxyethylene (20) sorbitan monooleate 1.0
Propylene glycol 7.0
Hyaluronic acid production promoter: Mitsubatsugi hot water extract (dry mass) 10.0
Sodium bisulfite 0.01
Ethylparaben 0.3
Perfume Appropriate amount of purified water

[Formulation Example 11: Cream]
(Compounding ingredients) (mass%)
Solid paraffin 5.0
Beeswax 10.0
Vaseline 15.0
Glycerin monostearate ester 2.0
Polyoxyethylene (20) sorbitan monolaurate 3.0
Soap powder 0.1
Borax 0.2
Hyaluronic acid production promoter: smelt methanol extract (dry mass) 0.05
Sodium bisulfite 0.03
Ethylparaben 0.3
Perfume Appropriate amount of purified water

[Composition Example 12: Drink (in 50 mL)]
Hyaluronic acid production promoter: Hot spring extract of hot spring (dry mass) 5mg
Hyaluronic acid production promoter: Barely hydrous ethanol extract (dry mass) 5mg
Collagen peptide 5mg
Pearl barley extract (dry mass) 2mg
Reduced maltose starch syrup 28mg
Erythritol 8mg
Citric acid 2mg
Fragrance 1.3mg
Hyaluronic acid 0.5mg
Vitamin E 0.3mg
Coenzyme Q10 1.2mg
Ceramide (konjac extract) 0.4mg
L-proline 2mg
Water residue

[Formulation Example 13: Tablet]
Sucrose ester 70mg
Crystalline cellulose 74mg
Methylcellulose 36mg
Glycerin 25mg
Black bear extract 300mg
Hyaluronic acid production promoter: Honey beetle water-containing ethanol extract (dry mass) 40 mg
Hyaluronic acid production promoter: Wedge water-containing ethanol extract (dry mass) 50mg
Collagen peptide 60mg
N-acetylglucosamine 180mg
Hyaluronic acid 150mg
α-Lipoic acid 20mg
Ceramide (konjac extract) 55mg
(Total) 1060mg

[Formulation Example 14: Soft capsule C]
Edible soybean oil 528mg
Hyaluronic acid production promoter: water extract of dried water of bonnet (dry mass) 50mg
Hyaluronic acid production promoter: Barely hot water extract (dry mass) 80mg
Collagen peptide 43mg
Lotus germ extract (dry mass) 16mg
Royal Jelly 70mg
Maca 60mg
Beeswax 60mg
Gelatin 375mg
Glycerin 113mg
Glycerin fatty acid ester 105mg
(Total) 1500mg

Claims (3)

  The hyaluronic acid production promoter characterized by including the 1 type (s) or 2 or more types chosen from the plant body or extract of Harigiri, honeybee, a wedge, and a spruce.   An anti-aging agent comprising one or two or more selected from a plant of Mitsubatsugi or Satsumabana or an extract thereof.   The wrinkle improving agent characterized by including the 1 type (s) or 2 or more types chosen from the plant body or the extract of a honeybee tsugi or radish.