JP2011195505A - Type-i collagen production promoter, anti-aging agent and wrinkle-ameliorating agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a type-I collagen production promoter, an anti-aging agent and a wrinkle-ameliorating agent each exhibiting an excellent type-I collagen production-promoting action.SOLUTION: The type-1 collagen production promoter, the anti-aging agent and the wrinkle-ameliorating agent each comprises one or two or more selected from among plant bodies or extracts thereof of Pleioblastus linearis Nakai, Ficus pumila Linn., and Tetragonia tetragonioides.

Description

  The present invention relates to a type I collagen production promoter, an anti-aging agent and a wrinkle improving agent having a type I collagen production promoting effect.

  The skin is composed of the stratum corneum, epidermis layer, basement membrane and dermis from the outside, and the dermis is the widest part of the skin. Collagenous fibers, elastic fibers, glycoproteins, and proteoglycans have a heterogeneous structure in which they are spread in a three-dimensional manner, and each structure is in a gel state with a liquid phase. Collagen fibers are mainly composed of collagen, of which type I collagen accounts for 80% of the total. In addition to type I collagen, the presence of type III, V, XII and XIV type collagen is known. Occurrence of wrinkles and sagging seen in aging skin is a major aging change in appearance, and is a serious problem for many middle-aged and elderly people. One of the causes of wrinkles and sagging is due to thinning of skin tissue with aging. In aging skin, there is a marked decrease in type I collagen fibers, the main matrix component of the dermis, and this is likely the main cause of skin thickness reduction. Therefore, it is considered effective to prevent and improve wrinkles and sagging by promoting the production of type I collagen and maintaining the amount of type I collagen. Furthermore, the promotion of type I collagen production is also effective in improving skin wound healing.

  Conventionally, as a component derived from a natural product that promotes the production of type I collagen to prevent and improve the aging change of the skin, for example, an isoflavone compound selected from daidzein, soydin, genistein, and genistin, phytosterol, Reference 1) and a specific phytoplankton extract (see Patent Document 2) have been reported.

JP 2001-39849 A JP 2007-186471 A

As described above, various extracts derived from plants have been developed as having an action to promote type I collagen production, with the expectation of their safety and mild irritation to the skin. There is a need for further development of a new plant that has not been found to exhibit the effect of promoting the production of a new type I collagen.
The present invention has been made in response to such a conventional situation, and an object thereof is to provide a new plant-derived type I collagen production promoter.

  As a result of intensive research in view of the present situation, the present inventors have found that certain plant extracts have an excellent type I collagen production promoting action and are useful as type I collagen production promoters. Was found to be useful as an anti-aging agent and a wrinkle-improving agent based on the type I collagen production promoting action, and the present invention has been completed.

  The present invention is a type I collagen production promoter characterized by comprising one or more selected from Ryukyu-chiku, Ooibi and Tsuruna plants or extracts thereof.

  Moreover, this invention is the anti-aging agent characterized by including the 1 type (s) or 2 or more types chosen from the plant body of Ryukyu-chiku, Ooitabi, and Tsuruna, or its extract.

  Furthermore, this invention is a wrinkle improving agent characterized by including the 1 type (s) or 2 or more types chosen from the plant body of a mushroom or a tsuruna or its extract.

  The type I collagen production promoter of the present invention is excellent in the effect of promoting type I collagen production and is safe. Therefore, according to the type I collagen production promoter of the present invention, it is possible to promote the production of type I collagen, maintain the amount of type I collagen, promote wrinkle / sag prevention and skin wound healing, It is effective in improving various symptoms caused by collagen shortage. In addition, since ingredients derived from natural products are used, it is safe even for internal use or external application to humans or animals.

  The anti-aging agent and wrinkle ameliorating agent based on the type I collagen production promoting action of the present invention promotes type I collagen production, prevents skin wrinkles and sagging, and maintains a moist and youthful skin state. Has the effect of being able to. In addition, since ingredients derived from natural products are used, it is safe even for internal use or external application to humans or animals.

Hereinafter, the present invention will be described in detail.
The type I collagen production promoter, anti-aging agent and wrinkle improving agent of the present invention have an excellent ability to promote type I collagen production. Therefore, the type I collagen production promoter, anti-aging agent and wrinkle-improving agent of the present invention are suitable as materials for pharmaceuticals (for example, external preparations for skin, internal medicines), quasi drugs, and cosmetics. Moreover, it is applicable also to food-drinks (for example, a supplement and a drink).

Ryukyuchiku (scientific name: Pleioblastus linearis Nakai) used in the present invention is a plant belonging to the genus Medaeaceae.
Japanese Patent Application Laid-Open No. 2007-230922 describes a collagen production promoter containing a bamboo genus of the genus Muraceae or Sasa genus (liquid coming out from the cut end of a bamboo stalk).

The giant gourd (scientific name: Ficus pumila Linn.) Used in the present invention is a plant belonging to the genus Figaceae.
Ootabi is said to cure numbness and pain caused by rheumatism, diarrhea, gonorrhea, and bruises (Makino Kazuhan Medical Herb Encyclopedia). In JP-A-11-60496, it is used as a hyaluronic acid producing ability enhancer, and in WO 07/94312, it is used as a vitamin C transporter production promoter.

The tuna (scientific name: Tetragonia tetragonioides) used in the present invention is a plant belonging to the genus Tsuruna belonging to the family Papaveraceae. There is also a classification of perennials of the Crassaceae family.
Tsuruna has antibacterial activity and is known to be effective in the private sector such as clean fever, detoxification, gout, antiseptic, healthy stomach, etc. Gastritis, enteritis, sepsis, etc. (Makino Wakahan Herb Encyclopedia). In China, the whole plant is dried to be used for detoxification and fever, and it is also used for cancer treatment (World useful plant encyclopedia).

Thus, there has never been a report that the plant body or plant extract of each plant described above has a type I collagen production-promoting action, which has been found for the first time by the present inventors.
In addition, it is not known that the plants or plant extracts of these plants have an anti-aging effect and a wrinkle improving effect.

  In addition, the plant body of each plant used in the present invention or an extract thereof refers to various parts of each plant body (flower, flower ear, fruit skin, fruit, stem, leaf, branch, branch leaf, trunk, bark, rhizome, root). Skin, roots, seeds, whole grass, etc.) as they are or dried and pulverized into dry powders, or as they are or after drying and pulverizing and then extracted with a solvent.

  In the case of an extract, the extraction solvent used for extraction may be any solvent that is usually used for extraction, in particular alcohols such as methanol, ethanol or 1,3-butylene glycol, hydrous alcohols, acetone, ethyl acetate, etc. These organic solvents can be used alone or in combination. Of these, alcohols and hydrous alcohols are particularly preferred, and methanol, ethanol, 1,3-butylene glycol, hydrous ethanol or hydrous 1,3-butylene glycol are particularly preferred. . The solvent is preferably used at a temperature between room temperature and the boiling point of the solvent.

  There are no particular limitations on the extraction method, but usually it may be in the range of the boiling point of the solvent at room temperature to normal pressure. After extraction, the solution is filtered, ion exchanged, adsorbed, decolored and purified. , Paste, gel, and powder. In many cases, it can be used as it is, but if necessary, further purification treatment such as deodorization and decoloration may be added as long as the effect is not affected. An activated carbon column or the like may be used, and usual means generally applied depending on the extracted substance may be arbitrarily selected.

  As the extraction site of the plant body, whole plants are preferred for Ryukyu-chiku, leaves, branches and berries for green oysters, and leaves and branches for tsuruna, but extracts from other parts can also be used.

  The type I collagen production promoter, anti-aging agent, and wrinkle improving agent of the present invention are preferably composed of one or more of the aforementioned plants or extracts thereof, but do not impair the effects of the present invention. Various other components can be contained in the range.

  The type I collagen production promoter, anti-aging agent, and wrinkle-improving agent of the present invention can be used in humans and animals by blending with external preparations for skin, as well as various foods and drinks, feeds (such as pet food). Can be ingested. It can also be administered to humans and animals as a pharmaceutical formulation. At this time, it may be adjusted by arbitrarily drying, concentrating or diluting depending on the dosage form and form of the external preparation for skin and food and drink.

  When the type I collagen production promoter, anti-aging agent, and wrinkle-improving agent of the present invention are blended in a skin external preparation, an extract of each plant is preferable, and the blending amount (dry mass) is generally within the total amount of the external preparation. 0.0001-10 mass% is preferable.

  When the present invention is applied to an external preparation for skin, in addition to the above components, components that are usually used in external preparations for skin such as cosmetics and pharmaceuticals within the range that does not impair the effects of the present invention, if necessary, such as antioxidants Oils, UV protection agents, surfactants, thickeners, alcohols, powder components, color materials, aqueous components, water, various skin nutrients, and the like can be appropriately blended as necessary.

  Furthermore, edetate disodium, edetate trisodium, sodium citrate, sodium polyphosphate, sodium metaphosphate, sequestering agents such as gluconic acid, preservatives such as methylparaben, ethylparaben, butylparaben, caffeine, tannin, Verapamil, tranexamic acid and its derivatives, licorice extract, grabrizine, hot water extract of karin fruit, various herbal medicines, tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, Whitening agents such as ascorbic acid glucoside, arbutin, and kojic acid, and sugars such as glucose, fructose, mannose, sucrose, and trehalose can also be appropriately blended.

  The external preparation for skin can be applied to cosmetics, quasi-drugs, etc., particularly preferably cosmetics applied to the outer skin, and the dosage form can also be applied to the skin. Any dosage form such as solution system, solubilization system, emulsification system, powder dispersion system, water-oil two-layer system, water-oil-powder three-layer system, ointment, lotion, gel, aerosol, etc. is applicable. Is done.

  The use form is also arbitrary, and for example, it can be used for facial cosmetics such as lotions, emulsions, creams and packs, makeup cosmetics such as foundations, lipsticks and eye shadows, aromatic cosmetics, bath preparations and the like.

  In addition, makeup cosmetics can be widely applied to foundations and the like, and toiletries such as body soaps and soaps. Furthermore, if it is a quasi-drug, it can be widely applied to various ointment forms. And the form which can take the type I collagen production promoter of this invention, an anti-aging agent, and a wrinkle improving agent is not limited to these dosage forms and forms.

  When the type I collagen production promoter, anti-aging agent, and wrinkle improving agent of the present invention are blended in foods and drinks, feeds, etc., the blending amount (dry mass) of the plant body or its extract is the type, purpose, It can be appropriately determined depending on the form, usage, etc. For example, in the case of tablets and capsules in the total amount of food and drink, 1 to 90% by weight is preferable, and 0.001 to 50% by weight for other food and drink Is preferred. It is preferable to prepare so that the daily intake of the plant or its extract per adult is about 1 to 1,000 mg. In particular, when used as a health food or drink, it is preferable to contain the active ingredient of the present invention in such an amount that a predetermined effect is sufficiently exhibited.

  As a form of food-drinks or feed, it can be arbitrarily shaped into, for example, granules, granules, pastes, gels, solids, or liquids. These include various known substances that are recognized to be contained in foods and drinks, such as binders, disintegrants, thickeners, dispersants, reabsorption accelerators, flavoring agents, buffers, and surfactants. Excipients such as solubilizers, preservatives, emulsifiers, tonicity agents, stabilizers and pH adjusters may be included as appropriate.

  When the type I collagen production promoter, anti-aging agent, and wrinkle improving agent of the present invention are used as pharmaceutical preparations, the preparations are appropriately used orally or parenterally (intravenous administration, intraperitoneal administration, etc.). The The dosage form is also arbitrary, for example, oral solid preparations such as tablets, granules, powders and capsules, oral liquid preparations such as internal liquids and syrups, and parenteral liquid preparations such as injections. These forms can also be appropriately prepared by known methods. For these pharmaceutical preparations, commonly used binders, disintegrants, thickeners, dispersants, reabsorption accelerators, corrigents, buffers, surfactants, solubilizers, preservatives, emulsifiers, isotonicity Excipients such as agents, stabilizers and pH adjusters may be used as appropriate.

When the type I collagen production promoter, anti-aging agent, and wrinkle improving agent of the present invention are used as an external preparation for skin, foods and drinks, pharmaceutical preparations, etc., the anti-aging of human skin is prevented by an excellent type I collagen production promoting action, Effectively used for skin agglomeration, elasticity retention, prevention and treatment of arthritis, etc., early treatment of burns, etc. When added to food, it is expected that the effect of promoting type I collagen production will be exerted from the inside by ingesting such food.
In addition, with the concept of physiological functions such as treatment, prevention, and improvement of the above symptoms and pathological conditions, it can be applied to external preparations for skin, functional foods and beverages, foods for specified health use, and the like.

  The method for adding the type I collagen production promoter, anti-aging agent, and wrinkle-improving agent of the present invention to an external preparation for skin or food and drink may be added in advance or during production. It may be selected appropriately considering workability.

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. Unless otherwise specified, the amount is shown in mass%.
First, a method for preparing a plant extract used in this example, a test method for promoting the production of type I collagen, and the results thereof will be described.

1. Sample preparation All samples were grown in Okinawa Prefecture and were obtained from Southeast Botanical Garden in Okinawa.
The following samples were prepared and used as test samples.
(1) Ryukyu-chiku extract 10.87 g of Ryukyu-chi whole dried product was immersed in 80 mL of methanol at room temperature for one week. Subsequently, the solvent was distilled off from the extract obtained by filtration with filter paper to obtain 654 mg (yield: 6.0%) of Ryukyu Methanol Extract.
(2) Green-spotted extract 10.3 g of green-striped leaves, branches, and dried fruits were immersed in 80 mL of methanol at room temperature for 1 week. Subsequently, the solvent was distilled off from the extract obtained by filtration with filter paper to obtain 753.1 mg (yield: 10.1%) of the oyster bimethanol extract.
(3) Tsuruna extract 10.42 g of dried tsuruna leaves and branches was immersed in 80 mL of methanol for one week at room temperature. Subsequently, the solvent was distilled off from the extract obtained by filtration with filter paper to obtain 1663.8 mg (yield: 16.0%) of tsuruna methanol extract.

2. Type I collagen production promoting effect test method and results 1. Using each plant extract obtained in the above as a test sample, the type I collagen production promoting effect was measured and evaluated by the following method.

(1) Test method of type I collagen production promoting action The type I collagen production promoting action of each sample was evaluated by the following method.
Human 0-year-old foreskin-derived dermal fibroblasts were seeded in a 24-well plate at 60000 cells / well. After overnight culture in 0.5 ml of 0.5% FBS-added DMEM medium, a test substance solution (solvent: DMSO) was added. Two days after the addition of the test substance solution, the medium was aspirated, and the amount of type I collagen in the collected supernatant was measured by ELISA (PIP EIA Kit, manufactured by TaKaRa).
As a result, the relative value of type I collagen with respect to the control when the control containing no test substance was defined as 100 was shown in Table 1.

As can be seen from Table 1, the plant extract of the present invention has an excellent type I collagen production promoting effect and is useful as a type I collagen production promoter and anti-aging agent.
Therefore, by providing the type I collagen production promoter of the present invention, a skin external preparation having an effect of promoting type I collagen production, an oral composition (for example, a functional agent, food and drink), a pharmaceutical preparation and the like are provided. It is useful for prevention, prevention, improvement, treatment, etc. of various symptoms, diseases, and pathological conditions.
Specific examples of application include prevention, improvement and treatment of skin aging such as sagging skin and wrinkles, and treatment of skin wounds. However, application is not limited to these examples.

  Hereinafter, formulation examples of the type I collagen production promoter according to the present invention in various dosage forms will be described as formulation examples. Needless to say, the present invention is not limited by these formulation examples and is specified by the scope of claims.

[Formulation Example 1: Candy]
2000mg sugar
Minamata 1926mg
Type I collagen production promoter: Ryukyu Chiku hot water extract (dry mass) 20mg
Collagen peptide 10mg
Pearl barley extract (dry mass) 2mg
Lotus germ extract (dry mass) 2mg
Fragrance 40mg
(Total) 4000mg

[Formulation Example 2: Tablet]
Sucrose ester 70mg
Crystalline cellulose 74mg
Methylcellulose 36mg
Glycerin 25mg
Black bear extract 300mg
Type I collagen production-promoting agent: agaric water-containing ethanol extract (dry mass) 120 mg
Collagen peptide 60mg
Pearl barley extract (dry mass) 10mg
N-acetylglucosamine 180mg
Hyaluronic acid 150mg
Vitamin E 30mg
Vitamin B6 20mg
Vitamin B2 10mg
α-Lipoic acid 20mg
Coenzyme Q10 40mg
Ceramide (konjac extract) 55mg
L-proline 300mg
(Total) 1500mg

[Formulation formulation example 3: soft capsule A]
Edible soybean oil 528mg
Type I collagen production promoter: Tsuruna water-containing ethanol extract (dry mass) 90 mg
Collagen peptide 45mg
Pearl extract (dry mass) 8mg
Lotus germ extract (dry mass) 16mg
Royal Jelly 70mg
Maca 60mg
GABA (γ-aminobutyric acid) 30mg
Beeswax 60mg
Gelatin 375mg
Glycerin 113mg
Glycerin fatty acid ester 105mg
(Total) 1500mg

[Formulation Example 4: Soft Capsule B]
Brown rice germ oil 650mg
Type I collagen production promoter: Ryukyu hot water extract (dry mass) 300mg
Collagen peptide 255mg
Pearl barley extract (dry mass) 50mg
Resveratrol 5mg
Elastin 180mg
DNA 30mg
Folic acid 30mg
(Total) 1500mg

[Formulation Example 5: Granules]
Type I collagen production-promoting agent: agaric hydrated ethanol extract (dry mass) 140 mg
Collagen peptide 70mg
Pearl barley extract (dry mass) 20mg
Lotus germ extract (dry mass) 40mg
Vitamin C 150mg
Soy isoflavone 270mg
Reduced lactose 360mg
Soybean oligosaccharide 36mg
Erythritol 36mg
Dextrin 30mg
Fragrance 24mg
Citric acid 24mg
(Total) 1200mg

[Composition Example 6: Drink (in 50 mL)]
Type I collagen production promoter: Tsuruna hot water extract (dry mass) 10mg
Collagen peptide 5mg
Pearl barley extract (dry mass) 2mg
Reduced maltose starch syrup 28mg
Erythritol 8mg
Citric acid 2mg
Fragrance 1.3mg
N-acetylglucosamine 1mg
Hyaluronic acid 0.5mg
Vitamin E 0.3mg
α-Lipoic acid 0.2mg
Coenzyme Q10 1.2mg
Ceramide (konjac extract) 0.4mg
L-proline 2mg
Water residue

[Formulation Example 7: Ointment]
(Compounding ingredients) (mass%)
Type I collagen production promoter: Ryukyu Methanol Extract (dry mass) 1.0
Stearyl alcohol 18.0
Owl 20.0
Polyoxyethylene (20) monooleate 0.25
Glycerin monostearate 0.3
Vaseline 40.0
Purified water residue

[Formulation formulation example 8: serum]
(Compounding ingredients) (mass%)
(Phase A)
95% ethyl alcohol 10.0
Polyoxyethylene (20) octyldodecanol 1.0
Pantothenyl ethyl ether 0.1
Type I collagen production-promoting agent: agaric hydrated ethanol extract (dry mass) 1.5
Methylparaben 0.15
(Phase B)
Potassium hydroxide 0.1
(Phase C)
Glycerin 5.0
Dipropylene glycol 10.0
Sodium bisulfite 0.03
Carboxyvinyl polymer 0.2
Purified water residue

[Composition example 9: Pack]
(Compounding ingredients) (mass%)
(Phase A)
Dipropylene glycol 5.0
Polyoxyethylene (60) hydrogenated castor oil 5.0
(Phase B)
Type I collagen production promoter: Tsuruna methanol extract (dry mass) 0.01
Olive oil 5.0
Tocopherol acetate 0.2
Ethylparaben 0.2
Fragrance 0.2
(Phase C)
Sodium bisulfite 0.03
Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
Ethanol 7.0
Purified water residue

[Formulation formulation example 10: emulsion]
(Compounding ingredients) (mass%)
Microcrystalline wax 1.0
Beeswax 2.0
Lanolin 20.0
Liquid paraffin 10.0
Squalane 5.0
Sorbitan sesquioleate ester 4.0
Polyoxyethylene (20) sorbitan monooleate 1.0
Propylene glycol 7.0
Type I collagen production promoter: Ryukyu hot water extract (dry mass) 10.0
Sodium bisulfite 0.01
Ethylparaben 0.3
Perfume Appropriate amount of purified water

[Formulation Example 11: Cream]
(Compounding ingredients) (mass%)
Solid paraffin 5.0
Beeswax 10.0
Vaseline 15.0
Glycerin monostearate ester 2.0
Polyoxyethylene (20) sorbitan monolaurate 3.0
Soap powder 0.1
Borax 0.2
Type I collagen production-promoting agent: Butterfly methanol extract (dry mass) 0.05
Sodium bisulfite 0.03
Ethylparaben 0.3
Perfume Appropriate amount of purified water

[Composition Example 12: Drink (in 50 mL)]
Type I collagen production promoter: Tsuruna hot water extract (dry mass) 5mg
Type I collagen production promoter: Ryukyuchi water-containing ethanol extract (dry mass) 5mg
Collagen peptide 5mg
Pearl barley extract (dry mass) 2mg
Reduced maltose starch syrup 28mg
Erythritol 8mg
Citric acid 2mg
Fragrance 1.3mg
Hyaluronic acid 0.5mg
Vitamin E 0.3mg
Coenzyme Q10 1.2mg
Ceramide (konjac extract) 0.4mg
L-proline 2mg
Water residue

[Formulation Example 13: Tablet]
Sucrose ester 70mg
Crystalline cellulose 74mg
Methylcellulose 36mg
Glycerin 25mg
Black bear extract 300mg
Type I collagen production-promoting agent: agaric water-containing ethanol extract (dry mass) 40 mg
Type I collagen production promoter: Tsuruna hydrous ethanol extract (dry mass) 50mg
Collagen peptide 60mg
N-acetylglucosamine 180mg
Hyaluronic acid 150mg
α-Lipoic acid 20mg
Ceramide (konjac extract) 55mg
(Total) 1060mg

[Formulation Example 14: Soft capsule C]
Edible soybean oil 528mg
Type I collagen production promoter: Ryukyuchi hydrous ethanol extract (dry mass) 50mg
Type I collagen production promoter: extract of hot water extract hot water (dry mass) 80mg
Collagen peptide 43mg
Lotus germ extract (dry mass) 16mg
Royal Jelly 70mg
Maca 60mg
Beeswax 60mg
Gelatin 375mg
Glycerin 113mg
Glycerin fatty acid ester 105mg
(Total) 1500mg

Claims (3)

  A type I collagen production promoter comprising one or two or more selected from Ryukyuchiku, Ooitabi and Tsuruna plants or extracts thereof.   An anti-aging agent comprising one or more selected from plants of Ryukyu Chiku, Ooi Tabi and Tsuruna or extracts thereof.   The wrinkle improving agent characterized by including the 1 type (s) or 2 or more types chosen from the plant body or plant extract of a giant mushroom or a tsuruna.