JP2002128632A - Cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prepare a cosmetic exhibiting excellent preventing and ameliorating effect on the aging and roughening of skin and having high safety. SOLUTION: An extract and/or crushed product of rice are added to a cosmetic. The cosmetic formulated with the extract and/or the crushed product of rice has antioxidation action and fibroblast cell activating action of the rice extract or crushed rice added as an additive and these actions are synergistically developed by the application of the cosmetic to the skin to exhibit excellent skin aging preventing effect and roughened skin improving effect compared with conventional antiaging cosmetic. Since the formulated component is originated from natural material, the cosmetic has low irritation to the skin and excellent safety.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cosmetic which exhibits an excellent effect of preventing or improving symptoms such as generation of wrinkles and wrinkles due to aging of the skin or rough skin, and which has high skin safety.

[0002]

2. Description of the Related Art Aging of the skin is caused by internal factors such as inactivation of cell proliferation and differentiation with aging and a decrease in hormone secretion.
It is a phenomenon that occurs when external factors such as cell or tissue damage or inflammation due to active oxygen induced by exposure to sunlight (ultraviolet rays) are intertwined in a complex manner. The morphological and physiological changes of the skin include wrinkles, sagging, and spots. It appears as symptoms such as rough skin. In order to prevent the aging of the skin and keep the skin healthy and youthful, various active ingredients have been conventionally used, and cosmetics containing these ingredients have been put on the market. For example, antioxidants such as vitamin Cs and vitamin Es, anti-inflammatory agents such as glycyrrhetinic acid, various ultraviolet absorbers, α-hydroxycarboxylic acid, placenta extract, γ-amino
Cell activation components such as -β-hydroxybutyric acid, collagen,
Examples thereof include skin matrix components such as elastin, and moisturizing agents such as hyaluronic acid and urea. However,
Since these ingredients generally only improve or eliminate one of the skin aging factors mentioned above,
At present, truly satisfactory skin aging prevention effects cannot be obtained with conventional cosmetics containing these components. Further, depending on the type of the component, there is a need for improvement in skin safety, such as a problem of skin irritation when the amount is increased in order to enhance the effectiveness.

[0003]

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned problems encountered in conventional cosmetics, and has as its object to reduce various factors such as skin aging and rough skin. An object of the present invention is to provide a cosmetic containing a new active ingredient that can maintain the skin in a healthy and youthful state by acting in a complex and multifaceted manner. Another object of the present invention is to provide the above-mentioned cosmetic composition which contains a component derived from a natural product as an active ingredient and has excellent skin safety. Still other objects of the present invention will become apparent from the description below.

[0004]

That is, the present invention relates to rice (Ory)
za sativa) extract and / or crushed product. Rice, which is incorporated in the cosmetic of the present invention in the form of an extract or a pulverized product, has both an antioxidant effect and a fibroblast activating effect as shown in Test Examples, and has a synergistic effect based on a combination of these effects. Effect
It is effective in preventing aging and rough skin or improving symptoms. In addition, it is needless to say that rice extract or pulverized matter has no skin irritation and is extremely safe for the skin without inquiring about the relationship between rice and rice from old times. No.

Hereinafter, the present invention will be described in detail. As the rice referred to in the present invention, any of the taxonomically belonging to Oryza sativa can be used, and specific examples thereof include Koshihikari, Sasanishiki, Nipponbare, Akita Komachi, Kinuhikari, Huachie-mae and the like. However, of course, the present invention is not limited to these. In the present invention, an extract obtained by extracting the whole rice, stem (culm), leaves, ears and the like of the rice with an appropriate solvent or an arbitrary part of the rice is crushed. Any one or two or more of the finely pulverized materials are blended into the cosmetic.
In this case, it is preferable to use the whole plant or leaf as the part to be used for rice, and it is particularly preferable to use the whole plant or leaf or rice leaf before heading in view of the effect. In addition, it is also possible to use those that have re-germinated and grown from a rice plant after cutting the rice.

[0006] Preparation of rice extract and pulverized product is carried out, for example, by the following method. That is, after washing the collected rice with water, a portion to be used, preferably a leaf, is cut into several cm to several tens of cm as needed, and preferably first subjected to a heat treatment, and the enzyme activity of the rice. To stop. As a result, the denaturation and alteration of the active ingredient contained in rice by enzymes are suppressed, and the activity of the collected rice during storage is reduced, or the activity of the rice extract or pulverized material over time is prevented. And a rice extract or pulverized product having high activity and good storage stability. Therefore, the heat treatment is particularly important for obtaining the cosmetics showing high effectiveness of the present invention, and it is important that the heat treatment be performed as soon as possible after collecting the rice. . It is difficult to determine by what time it is desirable to perform the heat treatment after rice collection, depending on the variety of rice, the timing of collection, and the like. It is desirable to perform the process while the green color is not completely faded and is still maintained. The heat treatment is preferably carried out by a wet heat treatment (steaming), and in the case of steaming, the time generally ranges from 10 seconds to 5 minutes, preferably from 15 seconds to 3 minutes, particularly preferably from 20 seconds to 1 minute. If the steaming time is longer than the above, the antioxidant activity and the like of the rice extract or pulverized product tend to decrease, which is not preferable.

After the heat treatment, the rice is subjected to an extraction treatment or crushed to obtain a crushed product. In the case of pulverized material, rice that has been subjected to the above heat treatment is
If necessary, cut into smaller pieces and then dry to adjust the water content to a value suitable for pulverization. In this case, the drying temperature is desirably set to a mild condition of 50 ° C. or less, preferably 40 ° C. or less. If the temperature is too high, the active components of rice may be deactivated. The pulverization after drying can be preferably performed by using an airflow pulverizer or the like. The range of the particle size of the pulverized product differs depending on the type of the cosmetic to be blended and the like and cannot be described unconditionally, but, for example, in the case of a cream, a milky lotion, etc., the blending property, the feel when applied to the skin, and From the viewpoint of effectiveness and the like, it is desirable that the powder is in the form of fine powder. On the other hand, in the case of a bath agent or the like, a powder of about several millimeters can be used.

[0008] The rice extract is prepared by immersing the rice, which has been subjected to the above-mentioned heat enzyme inactivation treatment, as it is, or, if necessary, further chopping it into an extraction solvent. The method is performed by, for example, mixing the substance with an extraction solvent. Examples of the extraction solvent include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, 1,2-pentanediol, and glycerin; ethyl acetate, acetic acid Butyl,
Esters such as methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene, and chloroform; It is preferable to use a mixture of water and lower alcohols or a mixture of water and polyhydric alcohols.

The extraction conditions vary depending on the site of use, shredding degree or particle size of the rice, the type of extraction solvent, and the like, but are generally 4 to 100 times, preferably 10 to 20 times the weight of rice. 1 to 7 hours at 5 to 80 ° C.
It is preferable to perform the extraction at a temperature of 20 to 60 ° C. for 2 to 48 hours. The extract solution obtained here is generally clarified by filtration and, if necessary, after adjusting the pH to 4 to 8,
It is used as it is or as an appropriate concentration by concentration under reduced pressure. In some cases, the powder may be used in the form of powder according to a conventional method such as a spray drying method and a freeze drying method.

The amount of the rice extract or pulverized product in the cosmetic of the present invention is generally 0.001 to 0.001 for basic cosmetics such as creams, emulsions and lotions.
0.5% by weight (as solid content; the same applies hereinafter), preferably 0.0%
In the range of 1 to 0.2% by weight, in the case of pulverized material, generally 0.01 to 5%
%, Preferably in the range of 0.1 to 2% by weight, and in the case of makeup cosmetics such as blusher, white powder, etc., in the case of extracts, generally 0.001 to 0.5% by weight, preferably 0.01 to 0.
1% by weight, generally 0.1 to 10% by weight, preferably 0.5 to 5% by weight in the case of pulverized material, and 0.001 to 0.5% by weight, preferably 0.01% in the case of hair cosmetic extract. In the case of a pulverized product, it is generally in the range of 0.01 to 5% by weight, preferably 0.1 to 2% by weight.
01 to 1% by weight, preferably 0.05 to 0.5% by weight; in the case of pulverized material, generally 1 to 50% by weight, preferably 5 to 5% by weight
It is in the range of 20% by weight.

The cosmetic of the present invention contains, in addition to the rice extract or pulverized product as an essential component, components commonly used in cosmetics, such as oily components, surfactants, humectants, thickeners, preservatives and sterilization. Agents, powder components, ultraviolet absorbers, antioxidants, dyes, fragrances, and the like are appropriately blended as necessary.

The oily component includes, for example, plant-derived oils and fats such as olive oil, jojoba oil, castor oil, soybean oil, coconut oil, palm oil, cacao oil and meadowfoam oil; mink oil, turtle oil and the like. Animal-derived fats and oils; waxes such as beeswax, carnauba wax and lanolin; hydrocarbons such as liquid paraffin, vaseline, paraffin wax and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid; Higher alcohols such as lauryl alcohol, cetanol and stearyl alcohol; synthetic esters such as isopropyl myristate, butyl oleate and 2-ethylhexyl glyceride; and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, and polyoxyethylene cured castor. Nonionic surfactants such as oils and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkyl benzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ethers Sulfate, polyoxyethylene alkyl ether phosphate, α-sulfonated fatty acid alkyl ester salt, polyoxyethylene alkyl phenyl ether phosphate Quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts , N, N-dialkyl morphonium salts, cationic surfactants such as polyethylene polyamine fatty acid amide salts; N, N-dimethyl-N-alkyl-N-carboxymethylammonio betaine;
N, N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N ',
And amphoteric surfactants such as N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

Examples of humectants include glycerin, propylene glycol, dipropylene glycol, 1,3-
Butylene glycol, polyethylene glycol, sorbitol, xylitol, pyrrolidone carboxy sodium and the like, further saccharides, hyaluronic acid and its derivatives,
Lactic acid, urea, higher fatty acid octyldodecyl, and the like.

Examples of the thickener include polysaccharides such as carrageenan, alginic acid, pectin, and locust bean gum; gums such as xanthan gum, tragacanth gum and guar gum; cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; polyvinyl alcohol, polyvinyl pyrrolidone; Synthetic polymers such as carboxyvinyl polymer and acrylic acid / methacrylic acid copolymer; hyaluronic acid and its derivatives;

Examples of the preservatives and bactericides include urea; paraoxybenzoates such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate and butyl paraoxybenzoate; phenoxyethanol, dichlorophen, hexachlorophen, and chlorhexidine hydrochloride. , Benzalkonium chloride, salicylic acid,
Examples include ethanol, undecylenic acid, phenols, and jamal (imidazodeinyl urea).

Examples of the powder component include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, and nylon powder.

Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and derivatives thereof, ethylhexyl paramethoxycinnamate, octyl cinnamate, 2,4-dihydroxybenzophenone, 2-hydroxy-4- Methoxybenzophenone-5-sulfonate, 2- (2-hydroxy-5-methylphenyl) benzotriazole,
There are urocanic acid and ethyl urocanate.

Examples of the antioxidant include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof (for example, vitamin E).
Nicotinate, vitamin E linoleate, etc.).
Further, as other components, lecithins, silk-related substances and the like can be blended.

The cosmetic of the present invention may further contain other active ingredients as long as the effectiveness of the components of the present invention is not impaired. Such compounds include, for example, kojic acid and its derivatives (eg, kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, kojic acid dibutyrate, etc.) and arbutin, ascorbic acid Acids and derivatives thereof [for example, ascorbic acid ester salts such as L-ascorbic acid-2-phosphate ester salt, L-ascorbic acid-2-sulfate ester salt (the salt is a sodium salt, a magnesium salt, etc.); L-ascorbic acid -2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-
O-α-D-glucopyranosyl-L-ascorbic acid)
Ascorbic acid glycosides, etc.], ellagic acid, resorcinol derivatives (e.g., 4-n-butyl resorcinol, 4-isoamyl resorcinol, etc.), soybean sap extract, yukinoshita extract, rice bran extract, 2,5-dihydroxy Whitening ingredients such as benzoic acid derivatives (eg, 2,5-diacetoxybenzoic acid etc.); placental extracts, collagen,
Nicotinic acid and its derivatives (eg, nicotinamide, benzyl nicotinate, etc.), α-hydroxy acids (eg, lactic acid, citric acid, α-hydroxyoctanoic acid, etc.), diisopropylamine dichloroacetate, γ-amino-β-
Hydroxy lactic acid and other ingredients for preventing skin aging and improving skin roughness;
There are crude drug extracts such as gentian extract.

The form of the cosmetic comprising the rice extract or pulverized product of the present invention is not particularly limited, and examples thereof include creams, emulsions, lotions, essences, facial cleansers, basic cosmetics such as packs, lipsticks, Makeup cosmetics such as foundations, liquid foundations, pressed powders, blushers and white powders, hair cosmetics such as hair restorers, treatments and tonics, and bath agents can be used.

Next, the present invention will be described more specifically with reference to Production Examples, Examples (formulation examples) and Test Examples, but the invention is not limited thereto. In the following, all parts are parts by weight, and all parts are by weight.

Production Example 1 Preparation of milled rice The rice (Koshihikari) collected just before heading (early stage)
Immediately after collecting the fresh leaves, the rice was cut into pieces, exposed to steam for 30 seconds, heat-treated, dried at 30 ° C., and then pulverized using an airflow pulverizer to prepare a pulverized rice product (particle size: about 5).
0 mesh).

Preparation Example 2 Preparation of Rice Extract Solution (1) 950 g of purified water was added to 50 g of the milled rice product obtained in Preparation Example 1.
And extracted at 40 ° C. for 3 hours, followed by filtration to obtain 720 ml of a tan transparent rice extract solution (solid content: 0.76%).

Production Example 3 Preparation of Rice Extract Solution (2) Purified water 900 g was added to 100 g of the rice crushed product obtained in Production Example 1.
g, and extracted at 40 ° C. for 6 hours, followed by filtration to obtain 660 ml of a brown and transparent rice extract solution (solid content: 1.53%).

Preparation Example 4 Preparation of Rice Extract Solution (3) In Preparation Example 2, purified water / 1,3-
Butylene glycol = 70/30 (volume ratio, the same applies hereinafter)
A yellow-brown transparent rice extract solution (700 ml) was obtained in the same manner as in Production Example 2 except that the mixture was used (solid content: 0.6).
6%).

Preparation Example 5 Preparation of Rice Extract Solution (4) Preparation Example 2 is the same as Preparation Example 2, except that a purified water / ethanol = 80/20 mixed solution is used instead of purified water and extraction is performed at room temperature for 3 days. 860 ml of a tan transparent rice extract solution was obtained in the same manner as in Example 2 (solid content: 0.70%).

Preparation Example 6 Preparation of Rice Extract Solution (5) In Preparation Example 2, purified water / 1,2-
750 ml of a yellow-brown transparent rice extract solution in the same manner as in Production Example 2 except that a pentanediol = 95/5 mixed solution was used.
(Solid content: 0.62%).

Preparation Example 7 Preparation of Rice Extract Solution (6) In Preparation Example 2, a yellow-brown transparent material was prepared in the same manner as in Preparation Example 2, except that Koshihikari seedling leaves were used instead of Koshihikari leaves immediately before heading. To obtain a rice extract solution (solid content: 0.89%).

Production Example 8 Preparation of rice extract powder 600 m of rice extract solution obtained in the same manner as in Production Example 2
was concentrated to 60 ml and freeze-dried to obtain 10.1 g of rice extract powder.

Example 1 Cream [A component] part Liquid paraffin 5.0 hexalane (Note 1) 4.0 Paraffin 5.0 Glyceryl monostearate 2.0 Polyoxyethylene (20) sorbitan monostearate 6.0 butyl Paraben 0.1 (Note 1) Glyceryl trioctanoate manufactured by Technoble Co., Ltd. [Component B] Extract solution of Production Example 2 5.0 Glycerin 5.0 Carboxymethyl monostearate 0.1 Methyl paraben 0.1 Moiston C ( Note 2) 1.0 Amount of purified water to be 100 parts in total. (Note 2) NMF component manufactured by Technoble Co., Ltd. [Component C] Perfume Appropriate amount After heating the above components A and B to 80 ° C. or higher, respectively, stirring Mixed. After cooling to 50 ° C., the C component was added and further stirred and mixed to prepare a cream.

Example 2 Emulsion [Component A] Part Liquid paraffin 6.0 Hexalane 4.0 Jojoba oil 1.0 Polyoxyethylene (20) sorbitan monostearate 2.0 Soybean lecithin 1.5 Methylparaben 0.15 Ethylparaben 0.03 [Component B] Extract solution of Production Example 4 5.0 Glycerin 3.0 1,3-butylene glycol 2.0 Carboxymethylcellulose 0.3 Sodium hyaluronate 0.01 Purified water Amount that makes the total amount 100 parts [Component C] Perfume Appropriate amount After heating the above components A and B to 80 ° C. or higher, they were mixed by stirring. After cooling to 50 ° C., the C component was added and further stirred and mixed to prepare an emulsion.

Example 3 Lotion [Component] Part Extract solution of Production Example 5 5.0 Ethanol 10.0 Glycerin 3.0 1,3-butylene glycol 2.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.1 Appropriate amount of perfume Purified water An amount that gives a total amount of 100 parts The above components were mixed to prepare a lotion.

Example 4 Pack [Components] Part Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.2 Extract solution of Production Example 6 5.0 Perfume Appropriate amount Purified water A pack was prepared by mixing the above components in an amount of 100 parts.

Example 5 Pressed Powder [Component A] Part Bengala 0.5 Yellow Iron Oxide 1.5 Black Iron Oxide 0.1 Titanium Oxide 10.0 Nylon Powder 4.0 Sericite Amount of 100 parts of Mica 23 0.0 Talc 25.0 Extract powder of Production Example 8 0.05 [Component B] Squalane 1.0 Methylpolysiloxane 4.0 Propylparaben 0.1 Dehydroacetic acid 0.1 Liquid paraffin 2.0 Perfume Appropriate A The component and the component B were mixed and stirred, respectively, and then sieved with a 200 mesh Tyler mesh sieve, and the obtained mixed powder was pressed into a mold to prepare a uniform press powder.

Example 6 Lipstick [Ingredient A] part Castor oil Amount to make the total amount 100 parts Octyldodecanol 5. 0 Lanolin 5.0 Liquid lanolin 5.0 Beeswax 4.0 Ozokerite 7.0 Candelilla wax 2.0 Carnauba wax 1.0 [Component B] Titanium oxide 1.0 Dye 4.0 Extract powder of Production Example 8 1 [Component C] Fragrance trace amount The above-mentioned component A and component B were each heated and then mixed and stirred. This was heated again, the above-mentioned component C was added, the mixture was poured into a mold, and quenched to prepare a lipstick.

Example 7 Liquid Foundation [Component A] part stearic acid 2.4 propylene glycol monostearate 2.0 cetostearyl alcohol 0.2 liquid lanolin 2.0 liquid paraffin 3.0 isopropyl myristate 8.5 propylparaben Suitable amount [Component B] Extract solution of Production Example 7 5.0 Sodium carboxymethylcellulose 0.2 Bentonite 0.5 Propylene glycol 4.0 Triethanolamine 1.1 Suitable amount of methylparaben Purified water Amount such that the total amount becomes 100 parts [Component C] Titanium oxide 8.0 Talc 4.0 Color pigment Appropriate amount The above-mentioned component A and component B were each heated and then mixed and stirred. The mixture was heated again, the above-mentioned component C was added, the mixture was poured into a mold, and the mixture was stirred until reaching room temperature to prepare a liquid foundation.

Example 8 Treatment [Ingredients] Part Stearyltrimethylammonium Chloride 6.0 Polyvinylpyrrolidone 4.0 Glycerin 1.0 Ethylparaben 0.1 Extract Solution of Production Example 2 5.0 Purified Water A total of 100 parts Amount The above components were heated to 80 ° C., and then mixed and stirred to prepare a treatment.

Example 9 Pack [Components] Part Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.2 Pulverized product of Production Example 1 1.0 Perfume Appropriate amount Purified water Total amount is 100 Parts Amount The above components were mixed to prepare a pack.

Example 10 Bath agent [Component] part Sodium sulfate 35.0 Sodium bicarbonate Amount of the total amount to be 100 parts Borax 2.0 Sodium carboxymethylcellulose 1.0 Red No. 1 Trace amount Fragrance Trace amount Pulverized product of Production Example 1 10.0

Example 11 Cream In Example 1,
A cream was prepared in the same manner as in Example 1 except that the rice extract solution of Production Example 7 was used instead of the rice extract solution of Production Example 2.

Comparative Example 1 Cream In Example 1, instead of 5.0 parts of the rice extract solution of Production Example 2, 0.1 part of α-tocopherol which is an existing anti-aging agent (antioxidant) was used. Except for using, a cream was prepared in the same manner as in Example 1.

Comparative Example 2 Cream In place of 5.0 parts of the rice extract solution of Production Example 2 in Example 1, 5.0 parts of a placenta extract, which is an existing anti-aging component (cell activator), was used instead of 5.0 parts of the rice extract solution. Except for using, a cream was prepared in the same manner as in Example 1.

Test Example 1 Antioxidant activity of rice [Sample] (1) A solution obtained by diluting the rice extract solution of Production Example 2 with purified water to 5.0% (2) A rice extract solution of Production Example 2 Is diluted with purified water to obtain 1
0.03% liquid (3) The rice extract solution of Preparation Example 7 was diluted to 5.0% with purified water. (4) The rice extract solution of Preparation Example 7 was diluted with purified water. 1
Liquid with 0.0%

[Test method] 1.0 ml of a 500 mM ethanol solution of linoleic acid, 0.2 N phosphate buffer (pH 6.
98) 10 ml, ethanol 9.0 ml and sample solution 5.0 ml were mixed to prepare an evaluation solution, which was kept at 40 ° C. With the preparation of the evaluation solution as day 0, the amount of lipid peroxide produced on days 3 and 7 was measured using the iron-rodin method. As a control, 5.0 ml of purified water (blank) or 5.0 ml of 40 μM tocopherol aqueous solution (positive control) was used instead of 5.0 ml of the sample solution in the above evaluation solution.
The same test was performed for the liquids each of which was mixed.

[Results] The results are shown in Table 1.

[Table 1]

As is evident from the results in Table 1, the extracts obtained from the leaves of rice before seeding and the leaves of rice seedlings both markedly suppress the production of lipid peroxide and exhibit a strong antioxidant effect. . In addition, the dried leaves obtained by exposing rice leaves before heading in the same manner as in Production Example 1 for 30 days on the sun without heat enzyme inactivation treatment, and the rice (Koshihikari) collected 10 days after heading were obtained. Using fresh leaves, a rice extract solution prepared by performing the same treatment as in Production 1 and 2, respectively.
When the lipid peroxide inhibitory effect was examined, the amount of lipid peroxide produced on day 4 was the same as that of the rice extract of Production Example 2 or 7.
The antioxidant power of the rice tended to decrease by 5 to 10 times, and the storage or heading for a long time after rice harvesting was observed.

Test Example 2 Activation of rice by fibroblasts [Sample] (1) Rice extract solution of Production Example 2 (2) Rice extract solution of Production Example 7

[Test Method] Human dermis-derived fibroblast NB1R
GB (000824) was seeded at 1 × 10 ⁴ cells / well in a 96-well microplate containing a minimum essential medium containing 0.5% FCS,
After pre-cultivation at 37 ° C. for 1 day, the sample solution was added to the medium.
It was added to a concentration of 5% or 5.0% and cultured at 37 ° C. for another 6 days. Next, remove the medium and remove the surfactant
After adding 0.2% MTT to the cell treatment solution to which (TRITON X-100) was added and maintaining at 37 ° C., using a microplate reader (Model 1450, manufactured by Bio-Rad),
The MTT value was measured at a wavelength of 370-630 nm. For comparison, a similar test was performed for the case where 50 mM glucose was added instead of the rice extract solution and the case where no sample was added (blank).

[Results] The results are shown in FIG. As shown in FIG. 1, the rice extract of the present invention showed an MTT against fibroblasts.
It has the effect of enhancing the activity and activating the cells.

Test Example 3 Skin irritation evaluation test The skin irritation of the rice extract was evaluated by a neutral red uptake test using cultured rabbit corneal cells (SIRC), which is an alternative to the ocular mucosal irritation test. For comparison, a similar evaluation was performed for α-tocopherol, which is a known antioxidant.

[Samples] (1) Extract powder of Production Example 8 (2) α-tocopherol (comparative sample)

[Test Method] SIRC was seeded at 5,000 cells / well in a 96-well microplate, and cultured in Eagle's minimum essential medium (MEM) containing 5% calf serum for 3 days. And then add 2
Cultured for days. Next, SIRC was cultured in an Eagle MEM medium containing 0.05% neutral red (NR) for 3 hours, the NR uptake rate was determined for the obtained SRIC, and the skin irritation of each sample was evaluated from the value. .

[Results] The results are shown in Table 2.

[Table 2]

As shown in Table 2, the rice extract of the present invention
Less irritating to the skin than existing antioxidants,
It is possible to provide cosmetics with excellent safety.

Test Example 4 Monitor Test For each of the creams of Examples 1 and 11 and Comparative Examples 1 and 2,
The effect on the skin was examined by a monitor test. [Test Method] Forty women, aged 18 to 50, randomly selected were divided into four groups of 10 subjects each of which was a subject, and each group was provided with one of four combinations of the creams of Examples and Comparative Examples. Allocate the cream of Example or Comparative Example on the left and right cheeks twice a day (morning,
Evening), after having been applied for one month, the effect of improving skin dullness and spots, the effect of improving fine wrinkles, and the effect of improving skin abrasion and gloss were evaluated based on the following evaluation criteria.

[Evaluation Criteria] (Improvement effect on skin dullness and spots) A: extremely improved B: considerably improved C: slightly improved D: unchanged E: rather noticeable ( A: Almost inconspicuous B: Remarkably inconspicuous C: Slightly inconspicuous D: No change E: Increased rather (Improvement effect on skin peel and gloss) A: Clearly improved B: considerably improved C: slightly improved D: not changed E: worsened

[Results] The results are shown in Table 3.

[0059]

[Table 3]

As shown in Table 3, the cream containing the rice extract of the present invention shows excellent effects of preventing skin aging and improving skin roughness, which are superior to conventional skin aging preventive cosmetics.

[0061]

Industrial Applicability The cosmetic of the present invention comprising a rice extract and / or pulverized product is characterized in that the rice extract or pulverized product as a blended component has both an antioxidant effect and a fibroblast activating effect. As a result of these actions acting synergistically at the time of application, they exhibit more excellent skin aging prevention and skin roughness improvement effects than conventional anti-aging cosmetics. In addition, since the ingredients are derived from natural products, they are less irritating to the skin and have excellent safety.

[Brief description of the drawings]

FIG. 1 is a graph showing the MTT value of each sample of Test Example 2 (vertical axis: MTT value).

Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat II (reference) A61P 17/00 A61P 17/00 (72) Inventor Yosuke Haneda 1-6-8 Kitahorie, Nishi-ku, Osaka Kyoei Chemical Industry In-house F Term (reference) 4C083 AA111 AA112 AA122 AB232 AB242 AB272 AB312 AB352 AB432 AB442 AC022 AC092 AC102 AC122 AC242 AC272 AC302 AC352 AC392 AC422 AC442 AC482 AC542 AC692 AD072 AD092 AD112 AD152 AD272 AD282 AD332 AD512 AD572 CC04 CC05 CC07 CC12 DD DD31 EE10 EE12 4C088 AB74 AC05 BA09 CA02 CA04 CA05 CA11 MA07 MA63 NA14 ZA89

Claims (5)

[Claims] 1. A cosmetic comprising a rice (Oryza sativa) extract and / or pulverized product. 2. The cosmetic according to claim 1, further comprising a rice extract and / or pulverized product which has been subjected to an enzyme inactivation treatment after collection and before fading. 3. The cosmetic according to claim 2, wherein the enzyme deactivation treatment is carried out by steam heat treatment for bringing rice into contact with steam. 4. The cosmetic according to claim 1, further comprising an extract and / or a crushed rice leaf. 5. The cosmetic according to claim 4, wherein rice leaves before heading are used as the rice leaves.