Nothing Special   »   [go: up one dir, main page]

FI20041124A - Selection marker gene free hybrid strains, process for their preparation, and use of these strains - Google Patents

Selection marker gene free hybrid strains, process for their preparation, and use of these strains Download PDF

Info

Publication number
FI20041124A
FI20041124A FI20041124A FI20041124A FI20041124A FI 20041124 A FI20041124 A FI 20041124A FI 20041124 A FI20041124 A FI 20041124A FI 20041124 A FI20041124 A FI 20041124A FI 20041124 A FI20041124 A FI 20041124A
Authority
FI
Finland
Prior art keywords
strains
selection marker
preparation
marker gene
dna
Prior art date
Application number
FI20041124A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI117388B (en
Inventor
Gerardus Cornelis Maria Selten
Bart Willem Swinkels
Gorcom Robertus Franciscus Van
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of FI20041124A publication Critical patent/FI20041124A/en
Application granted granted Critical
Publication of FI117388B publication Critical patent/FI117388B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
FI20041124A 1993-07-23 2004-08-27 Selection marker gene free yeast strains, process for their preparation and use of these strains FI117388B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93202195 1993-07-23
EP93202195 1993-07-23

Publications (2)

Publication Number Publication Date
FI20041124A true FI20041124A (en) 2004-08-27
FI117388B FI117388B (en) 2006-09-29

Family

ID=8214006

Family Applications (2)

Application Number Title Priority Date Filing Date
FI943485A FI114481B (en) 1993-07-23 1994-07-22 Selection marker gene-free filamentous fungal strains, process for their preparation, and use of these strains
FI20041124A FI117388B (en) 1993-07-23 2004-08-27 Selection marker gene free yeast strains, process for their preparation and use of these strains

Family Applications Before (1)

Application Number Title Priority Date Filing Date
FI943485A FI114481B (en) 1993-07-23 1994-07-22 Selection marker gene-free filamentous fungal strains, process for their preparation, and use of these strains

Country Status (13)

Country Link
US (3) US5876988A (en)
EP (2) EP1321523A3 (en)
JP (2) JP3833725B2 (en)
CN (1) CN100347301C (en)
AT (1) ATE238425T1 (en)
BR (1) BR9402912A (en)
CA (1) CA2128589C (en)
DE (1) DE69432543T2 (en)
DK (1) DK0635574T3 (en)
ES (1) ES2198410T3 (en)
FI (2) FI114481B (en)
IE (1) IE20030875A1 (en)
NZ (2) NZ299139A (en)

Families Citing this family (170)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY134684A (en) 1995-05-15 2007-12-31 Gist Brocades Bv Application of phospholipases in animal feed
NZ311181A (en) * 1995-06-23 2000-02-28 Danisco Ingredients As Obtaining metabolic mutants involving random mutations and specific selection not having recombinant DNA and a nucleic acid cassette encoding a bidirectional marker, and inducible enhancer and a basic transcription unit
ES2320281T3 (en) 1995-08-03 2009-05-20 Dsm Ip Assets B.V. GEN AMDS OF ASPERGILLUS NIGER THAT CODIFIES FOR AN ACETAMIDASE.
EP0814165B1 (en) * 1996-06-21 2003-04-09 Suntory Limited Method for constructing transformed yeast cells, that do not contain a selective marker gene
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
JP4531868B2 (en) * 1997-04-11 2010-08-25 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ Yeast cells, particularly by Kluyveromyces, comprising at least two copies of the desired gene integrated in the chromosomal genome of the domain encoding more than one non-ribosomal RNA
DK0979294T3 (en) 1997-04-11 2015-08-31 Dsm Ip Assets Bv Gene conversion AS A TOOL FOR CONSTRUCTION OF RECOMBINANT INDUSTRIAL filamentous fungi
US6558937B1 (en) 1997-07-31 2003-05-06 Dsm N.V. Cellulose degrading enzymes of aspergillus
CN1284998A (en) * 1997-12-22 2001-02-21 尤尼利弗公司 A process for site-directed integration of multiple copies of gene in mould
JP4410413B2 (en) * 1997-12-22 2010-02-03 コニンクリーケ デーエスエム ナムローゼ フェンノートシャップ Expression cloning in filamentous fungi
RU2230790C2 (en) 1998-05-19 2004-06-20 Дсм Н.В. Method for widening 5-membered ring of compound beta-lactam to 6-membered cephem
PL201350B1 (en) * 1998-07-16 2009-04-30 Novo Nordisk As Method of making proteins in transformed yeast cells
US6358705B1 (en) 1998-07-16 2002-03-19 Novo Nordisk A/S Method of making proteins in transformed yeast cells
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
US6750379B2 (en) 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
US7514110B1 (en) 2000-09-21 2009-04-07 Basf Aktiengesellschaft Talaromyces xylanases
BR0306740A (en) 2002-01-23 2004-12-28 Royal Nedalco B V Host cell transformed with a nucleic acid construct, isolated nucleic acid molecule, and processes for producing ethanol, and a fermentation product
US7517671B2 (en) * 2002-02-28 2009-04-14 New England Biolabs, Inc. Methods and compositions for concentrating secreted recombinant protein
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
AU2003285818A1 (en) * 2002-11-28 2004-06-18 Universiteit Leiden A method for marker-less integration of a sequence of interest into the genome of a cell
ES2400562T3 (en) 2003-05-28 2013-04-10 Dsm Sinochem Pharmaceuticals Netherlands B.V. Cefem compound
CN1298213C (en) * 2003-09-10 2007-02-07 王海波 Technology of obtaining gene transferred plant without selecting mark through division process
EP1673380A2 (en) * 2003-10-14 2006-06-28 DSM IP Assets B.V. Method for preparing a modified host cell
ATE480632T1 (en) 2004-04-02 2010-09-15 Dsm Ip Assets Bv FILAMENTOUS FUNGAL MUTANTS WITH IMPROVED HOMOLOGUE RECOMBINATION EFFICIENCY
CA2560205A1 (en) 2004-04-16 2005-10-27 Dsm Ip Assets B.V. Fungal promoters for expressing a gene in a fungal cell
JP2008516594A (en) * 2004-10-15 2008-05-22 ディーエスエム アイピー アセッツ ビー．ブイ． Homologous AMDS genes as selectable markers
EP2284257A1 (en) 2005-01-18 2011-02-16 Vereniging voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek en Patiëntenzorg Mycobacteria expressing a cap-specific mannosyl transferase
CA2595056A1 (en) 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
EP2458008A1 (en) 2005-11-29 2012-05-30 DSM IP Assets B.V. DNA binding site of a transcriptional activator useful in gene expression
US8637280B2 (en) 2005-12-01 2014-01-28 Adcuram Nutrition Holding Gmbh Genes useful for the industrial production of citric acid
BRPI0713795B1 (en) 2006-06-29 2018-03-20 Dsm Ip Assets B.V. Method of optimizing a coding nucleotide sequence encoding a predetermined amino acid sequence
EP2082046A2 (en) 2006-11-02 2009-07-29 DSMIP Assets B.V. Improved production of secreted proteins by filamentous fungi
EP2631295A3 (en) 2007-02-15 2014-01-01 DSM IP Assets B.V. A recombinant host cell for the production of a compound of interest
DK2126100T3 (en) 2007-03-21 2013-01-14 Dsm Ip Assets Bv Improved method of homologous recombination
WO2009065779A1 (en) 2007-11-20 2009-05-28 Dsm Ip Assets B.V. Dicarboxylic acid production in a recombinant yeast
US20110318795A1 (en) 2007-11-20 2011-12-29 Verwaal Rene Dicarboxylic acid production in a filamentous fungus
CA2704654C (en) 2007-11-20 2015-02-10 Dsm Ip Assets B.V. Succinic acid production in a eukaryotic cell
EP3037541A1 (en) 2007-12-17 2016-06-29 Photanol B.V. Light-driven co2 reduction to organic compounds to serve as fuels or as industrial half products by an autotroph containing a fermentative gene cassette
FR2927089B1 (en) 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra METHOD OF TARGETED INTEGRATION OF MULTICOPIES OF A GENE OF INTEREST IN A YARROWIA STRAIN
EP2116136A1 (en) 2008-05-08 2009-11-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Novel phytases
WO2010004141A2 (en) * 2008-07-11 2010-01-14 Institut National De La Recherche Agronomique (Inra) New mutant yeast strains capable of accumulating a large quantity of lipids
CN102099466A (en) * 2008-07-29 2011-06-15 丹尼斯科美国公司 Increased production of aspartic proteases in filamentous fungal cells
EP2392649A3 (en) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA producing strains
DK2406372T3 (en) 2009-03-10 2017-11-27 Dsm Ip Assets Bv PRAGASTRIC ESTERASE AND DERIVATIVES THEREOF
CN102741421B (en) 2009-03-10 2015-03-25 帝斯曼知识产权资产管理有限公司 Method for improving the yield of a polypeptide
EP2408907B8 (en) 2009-03-17 2018-04-18 Dutch DNA Biotech B.V. Use of cellulose and ligno-cellulose active proteins
US9353387B2 (en) 2009-04-15 2016-05-31 Dsm Ip Assets B.V. Dicarboxylic acid production process
US20120041171A1 (en) 2009-04-22 2012-02-16 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
CN102439145B (en) 2009-04-24 2014-02-19 帝斯曼知识产权资产管理有限公司 Carbohydrate degrading polypeptide and uses thereof
UA108853C2 (en) 2009-07-10 2015-06-25 Galactose fermentation method
WO2011009700A1 (en) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
RU2567000C2 (en) 2009-10-22 2015-10-27 Басф Се Phytase, its application, feed and feed additive for animals, containing it
BR112012011596B1 (en) 2009-11-04 2018-10-02 Dsm Ip Assets Bv talaromyces transformant, processes for producing a talaromyces transformant, a multiple talaromyces transformant and a polypeptide composition comprising one or more cellulases, and processes for saccharifying lignocellulosic material and preparing a fermentation product
WO2011095374A2 (en) 2010-02-04 2011-08-11 Dsm Ip Assets B.V. Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains
AU2011214327B2 (en) 2010-02-11 2014-09-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
CA2789301A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
EA201201449A1 (en) 2010-04-21 2013-03-29 ДСМ АйПи АССЕТС Б.В. METHOD OF OBTAINING CELLS ABOVABLE TO CONVERT ARABINOSE
EP2388331A1 (en) 2010-05-21 2011-11-23 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Constitutive promoter
CN102971419B (en) 2010-06-29 2015-02-11 帝斯曼知识产权资产管理有限公司 Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof
DK2588494T3 (en) 2010-06-29 2018-06-14 Dsm Ip Assets Bv POLYPEPTIDE WITH BETA-GLUCOSIDASE ACTIVITY AND APPLICATIONS THEREOF
EP2588600A1 (en) 2010-06-29 2013-05-08 DSM IP Assets B.V. Polypeptide having acetyl xylan esterase activity and uses thereof
US9109213B2 (en) 2010-06-29 2015-08-18 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
US9175050B2 (en) 2010-06-29 2015-11-03 Dsm Ip Assets B.V. Polypeptide having swollenin activity and uses thereof
CN102971338A (en) 2010-06-29 2013-03-13 帝斯曼知识产权资产管理有限公司 Polypeptide having beta-glucosidase activity and uses thereof
CN103459600B (en) 2010-07-01 2016-10-19 帝斯曼知识产权资产管理有限公司 For the method producing compound interested
CA2813513A1 (en) 2010-10-13 2012-04-19 Dsm Ip Assets B.V. Polypeptides with permease activity
CN103596970A (en) 2011-03-11 2014-02-19 帝斯曼知识产权资产管理有限公司 Vector-host system
HUE027001T2 (en) 2011-04-21 2016-10-28 Basf Se Synthetic phytase variants
EP2699589A2 (en) 2011-04-22 2014-02-26 DSM IP Assets B.V. Yeast cell capable of converting sugars including arabinose and xylose
EP2554668A1 (en) 2011-08-04 2013-02-06 DSM IP Assets B.V. A pentose sugar fermenting cell
AR087423A1 (en) 2011-08-04 2014-03-19 Dsm Ip Assets Bv CAPABLE CELL TO FERMENT PENTOUS SUGARS
WO2013110673A1 (en) 2012-01-23 2013-08-01 Dsm Ip Assets B.V. Diterpene production
WO2013189878A1 (en) 2012-06-19 2013-12-27 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
DK2875119T3 (en) 2012-07-19 2018-04-03 Dsm Ip Assets Bv AGSE-DEFICIENT TRIALS
ES2717682T3 (en) 2012-10-16 2019-06-24 Dsm Ip Assets Bv Cells with improved pentose conversion
WO2014096436A1 (en) * 2012-12-21 2014-06-26 Estibio Aps Marker-free genetically-engineered double mutants of thermoanaerobacter
EP3447136B1 (en) 2013-02-04 2020-05-13 DSM IP Assets B.V. Carbohydrate degrading polypeptide and uses thereof
EP2772545A1 (en) 2013-03-01 2014-09-03 Nederlandse Organisatie voor toegepast -natuurwetenschappelijk onderzoek TNO Starch active proteins
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014161988A1 (en) 2013-04-05 2014-10-09 Université Du Luxembourg Biotechnological production of itaconic acid
WO2014178717A1 (en) 2013-05-02 2014-11-06 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Novel organic acid pathway
US10689681B2 (en) 2013-05-31 2020-06-23 Dsm Ip Assets B.V. Microorganisms for diterpene production
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
CN108064135A (en) 2013-07-15 2018-05-22 帝斯曼知识产权资产管理有限公司 The production of diterpene
EP3027632B1 (en) 2013-07-31 2019-03-20 DSM IP Assets B.V. Recovery of steviol glycosides from a fermentation broth
DK3077517T3 (en) 2013-12-02 2019-02-11 Dsm Ip Assets Bv ISSTRUCTURING PROTEIN
WO2016012429A1 (en) 2014-07-24 2016-01-28 Dsm Ip Assets B.V. Yeast cell with improved pentose transport
FR3028527A1 (en) 2014-11-13 2016-05-20 Pivert IDENTIFICATION OF TRANSCRIPTION FACTORS OF YARROWIA LIPOLYTICA
EP3242950B1 (en) 2015-01-06 2021-10-06 DSM IP Assets B.V. A crispr-cas system for a filamentous fungal host cell
EP3242949B1 (en) 2015-01-06 2021-11-03 DSM IP Assets B.V. A crispr-cas system for a yeast host cell
DK3242948T3 (en) 2015-01-06 2022-03-07 Dsm Ip Assets Bv CRISPR CAS SYSTEM FOR A YARROWIA HOST CELL
BR112017016821A2 (en) 2015-02-06 2018-04-03 Cargill Inc polypeptide, nucleic acid, vector, host cell, fermentation medium, use of polypeptide, feed composition and fermentation method
CN107429273A (en) 2015-02-16 2017-12-01 帝斯曼知识产权资产管理有限公司 Method for producing itaconic acid under anaerobic
CA2979931A1 (en) 2015-03-16 2016-09-22 Dsm Ip Assets B.V. Udp-glycosyltransferases
WO2016151046A1 (en) 2015-03-23 2016-09-29 Dsm Ip Assets B.V. Udp-glycosyltransferases from solanum lycopersicum
CN107429220A (en) 2015-03-27 2017-12-01 嘉吉公司 Yeast strain through glucose starch enzyme modification and the method for producing biologic
EP3718417A1 (en) 2015-04-03 2020-10-07 DSM IP Assets B.V. Steviol glycosides
CN107532135A (en) 2015-04-21 2018-01-02 帝斯曼知识产权资产管理有限公司 The micro-organisms of terpenoid
MY186983A (en) 2015-05-22 2021-08-26 Dsm Ip Assets Bv Acetate consuming yeast cell
US20180160695A1 (en) 2015-06-02 2018-06-14 Dsm Ip Assets B.V. Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
US11066688B2 (en) 2015-07-10 2021-07-20 Dsm Ip Assets B.V. Method for preparing a steviol glycoside composition
WO2017024150A1 (en) 2015-08-05 2017-02-09 Cargill, Incorporated Xylose isomerase-modified yeast strains and methods for bioproduct production
CA2993744A1 (en) 2015-08-13 2017-02-16 Dsm Ip Assets B.V. Steviol glycoside transport
JP2018526998A (en) 2015-09-21 2018-09-20 ピュラック バイオケム ビー． ブイ． Production of FDCA by fungi
WO2017050652A1 (en) 2015-09-25 2017-03-30 Dsm Ip Assets B.V. Asparaginase
EP4043558A3 (en) 2015-10-05 2022-11-16 DSM IP Assets B.V. Kaurenoic acid hydroxylases
ES2767728T3 (en) * 2015-10-06 2020-06-18 Dsm Ip Assets Bv Eukaryotic cell with high production of fermentation product
WO2017091614A1 (en) 2015-11-24 2017-06-01 Cargill Incorporated Genetically modified yeasts and fermentation processes using genetically modified yeasts
JP2019503168A (en) 2015-12-02 2019-02-07 ビーエーエスエフ ソシエタス・ヨーロピアＢａｓｆ Ｓｅ Method for producing a protein in a filamentous fungus with reduced CLR2 activity
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
JP6605042B2 (en) 2015-12-07 2019-11-13 ザイマージェン インコーポレイテッド Improvement of microbial strain by HTP genome manipulation platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
JP6821598B2 (en) 2015-12-07 2021-01-27 ザイマージェン インコーポレイテッド Promoter derived from Corynebacterium glutamicum
JP2019500041A (en) 2015-12-30 2019-01-10 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． Partial enzymatic hydrolysis of triacylglycerol.
WO2017115322A2 (en) 2015-12-30 2017-07-06 Dsm Ip Assets B.V. Partial enzymatic hydrolysis of triacylglycerols
WO2017167848A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
EP3435772A1 (en) 2016-03-31 2019-02-06 DSM IP Assets B.V. Enzyme composition and preparation of a dairy product with improved properties
WO2017167847A1 (en) 2016-03-31 2017-10-05 Dsm Ip Assets B.V. Production of milk with low lactose content
WO2017216136A1 (en) 2016-06-14 2017-12-21 Dsm Ip Assets B.V. Recombinant yeast cell
JP2019519242A (en) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド Method for generating a bacterial hemoglobin library and its use
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. Methods for generating a glucose permease library and uses thereof
EP3485004B1 (en) 2016-07-13 2021-09-22 DSM IP Assets B.V. Malate dehyrogenases
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
CA3032736A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
CN109563117A (en) 2016-08-09 2019-04-02 帝斯曼知识产权资产管理有限公司 The crystallization of steviol glycoside
BR112019002417B1 (en) 2016-08-09 2023-05-02 Dsm Ip Assets B.V. CRYSTALLIZATION OF STEVIOL GLYCOSIDES
EP3504336A1 (en) 2016-08-26 2019-07-03 Lesaffre et Compagnie Improved production of itaconic acid
WO2018073107A1 (en) 2016-10-19 2018-04-26 Dsm Ip Assets B.V. Eukaryotic cell comprising xylose isomerase
AU2017350309A1 (en) 2016-10-27 2019-05-02 Dsm Ip Assets B.V. Geranylgeranyl pyrophosphate synthases
US11104886B2 (en) 2016-12-08 2021-08-31 Dsm Ip Assets B.V. Kaurenoic acid hydroxylases
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114941A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114940A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114995A1 (en) 2016-12-22 2018-06-28 Dsm Ip Assets B.V. Fermentation process for producing steviol glycosides
CA3048658A1 (en) * 2016-12-30 2018-07-05 Zymergen Inc. A method to build fungal production strains using automated steps for genetic manipulation and strain purification
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
EP3378931A1 (en) 2017-03-21 2018-09-26 Purac Biochem B.V. Fdca-decarboxylating monooxygenase-deficient host cells for producing fdca
EP3635112A2 (en) 2017-06-06 2020-04-15 Zymergen, Inc. A htp genomic engineering platform for improving fungal strains
CA3064607A1 (en) 2017-06-06 2018-12-13 Zymergen Inc. High throughput transposon mutagenesis
EP3638770B1 (en) 2017-06-13 2023-01-18 DSM IP Assets B.V. Recombinant yeast cell
CN110892068B (en) 2017-06-27 2024-03-08 帝斯曼知识产权资产管理有限公司 UDP-glycosyltransferase
CN111133111A (en) 2017-09-26 2020-05-08 帝斯曼知识产权资产管理有限公司 Acetic acid consuming strains
EP3533878A1 (en) 2018-02-28 2019-09-04 Dutch DNA Biotech B.V. Process for producing citramalic acid employing aspergillus
EP3790946B1 (en) 2018-05-07 2024-10-09 DSM IP Assets B.V. Process for enzymatic oil degumming
US11028401B2 (en) 2018-06-06 2021-06-08 Zymergen Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
US20210115418A1 (en) 2018-06-19 2021-04-22 Dsm Ip Assets B.V. Lipolytic enzyme variants
US20220389458A1 (en) 2019-11-04 2022-12-08 Dsm Ip Assets B.V. Low volume transfection
US20230212634A1 (en) 2020-01-14 2023-07-06 The Protein Brewery B.V. Expression of Ovalbumin and its Natural Variants
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
AU2021367159A1 (en) 2020-10-22 2023-05-04 Cargill, Incorporated Microorganisms for diterpene production
EP4023757A1 (en) 2020-12-29 2022-07-06 The Protein Brewery B.V. Methods for generating selection marker-free transformants of heterokaryotic organisms
WO2022269549A2 (en) 2021-06-24 2022-12-29 Fonterra Co-Operative Group Limited Recombinant proteins
WO2023285282A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
EP4370688A1 (en) 2021-07-12 2024-05-22 Danisco Us Inc Recombinant yeast cell
MX2024000511A (en) 2021-07-12 2024-03-11 Danisco Us Inc Recombinant yeast cell.
WO2023285280A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
MX2024000515A (en) 2021-07-12 2024-03-11 Danisco Us Inc Recombinant yeast cell.
MX2024000606A (en) 2021-07-12 2024-01-30 Danisco Us Inc Recombinant yeast cell.
MX2024005281A (en) 2021-11-04 2024-05-17 Danisco Us Inc Recombinant yeast cell.
CN118234857A (en) 2021-11-04 2024-06-21 丹尼斯科美国公司 Variant polypeptides and recombinant yeast cells
CN118215740A (en) 2021-11-04 2024-06-18 丹尼斯科美国公司 Method and recombinant yeast cell for producing ethanol
WO2023086793A1 (en) 2021-11-09 2023-05-19 Amgen Inc. Production of therapeutic proteins
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2023242273A1 (en) 2022-06-14 2023-12-21 Newmilkbuzz B.V. Expression of milk caseins in filamentous fungi
WO2024133849A1 (en) 2022-12-22 2024-06-27 The Protein Brewery B.V. Ovalbumin fermentation
WO2024218244A1 (en) 2023-04-20 2024-10-24 Dsm Ip Assets B.V. Strains and methods for the production of mogrosides

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422267A (en) * 1984-05-22 1995-06-06 Robert R. Yocum Industrial yeast comprising an integrated glucoamylase gene
AU607398B2 (en) * 1985-08-29 1991-03-07 Genencor Inc. Heterologous polypeptides expressed in filamentous fungi, processes for making same, and vectors for making same
US5298405A (en) * 1986-04-30 1994-03-29 Alko Limited Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production
US5079145A (en) * 1988-10-21 1992-01-07 Synergen Associates, Inc. Process for preparing microorganisms used for making phenyl acetyl carlinol (pac)
GB8904762D0 (en) * 1989-03-02 1989-04-12 Glaxo Group Ltd Biological process
US5695976A (en) * 1989-12-18 1997-12-09 Novo Nordisk A/S Stable integration of DNA in bacterial genomes
US5498830A (en) * 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5824513A (en) * 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
EP0498137A1 (en) * 1991-02-06 1992-08-12 Novo Nordisk A/S Novel expression systems
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
EP0565172A1 (en) * 1992-04-10 1993-10-13 Quest International B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having sulfhydryl oxidase activity
CA2096289A1 (en) * 1992-05-15 1993-11-16 Wouter Musters Cloning and expression of dna encoding a ripening form of a polypeptide having rhamnogalacturonase activity
US6291205B1 (en) * 1992-06-12 2001-09-18 Merck & Co., Inc. Method of increasing production of disulfide bonded recombinant proteins by saccharomyces cerevisiae
US6110713A (en) * 1993-03-23 2000-08-29 Regents Of The University Of Minnesota Production of glutamic acid and lysine using auxotrophic mutants of Bacillus methanolicus

Also Published As

Publication number Publication date
AU690909B2 (en) 1998-05-07
CA2128589A1 (en) 1995-01-24
DE69432543T2 (en) 2003-12-24
JPH07147971A (en) 1995-06-13
FI117388B (en) 2006-09-29
JP3833725B2 (en) 2006-10-18
FI114481B (en) 2004-10-29
US5876988A (en) 1999-03-02
AU6862894A (en) 1995-02-02
FI943485A (en) 1995-01-24
CA2128589C (en) 2005-10-11
DE69432543D1 (en) 2003-05-28
CN100347301C (en) 2007-11-07
EP0635574B1 (en) 2003-04-23
CN1098743A (en) 1995-02-15
EP1321523A3 (en) 2004-03-03
US6063614A (en) 2000-05-16
ATE238425T1 (en) 2003-05-15
JP2005296019A (en) 2005-10-27
EP1321523A2 (en) 2003-06-25
NZ299139A (en) 1998-06-26
DK0635574T3 (en) 2003-08-11
US6955909B1 (en) 2005-10-18
BR9402912A (en) 1995-04-11
ES2198410T3 (en) 2004-02-01
NZ264065A (en) 1998-06-26
IE940591A1 (en) 1995-01-25
EP0635574A1 (en) 1995-01-25
IE20030875A1 (en) 2004-01-14
FI943485A0 (en) 1994-07-22

Similar Documents

Publication Publication Date Title
FI20041124A (en) Selection marker gene free hybrid strains, process for their preparation, and use of these strains
Fujita et al. Structure of the dnaA region of Micrococcus luteus: conservation and variations among eubacteria
EP0331939A3 (en) Method and compositions for conferring resistance to retroviral infection
Kramer et al. Isolation of bacteriophage λ containing yeast ribosomal RNA genes: Screening by in situ RNA hybridization to plaques
DE69636866D1 (en) Reverse "two-hybrid"-systeme
ATE74962T1 (en) INSECT RESISTANT PLANTS.
EP0874055A3 (en) Method for the protection of plants against pathogens
AU654471B2 (en) Fungal resistant plants, process for obtaining fungal resistant plants and recombinant polynucleotides for use therein
ES2119744T3 (en) NITRATE REDUCTASE AS A MARKER OF ACREMONIUM CHRYSOGENUM.
AU1884888A (en) Inducible virus resistance in plants
CR20220673A (en) Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing
Reyes et al. Expression of human β-interferon cDNA under the control of a thymidine kinase promoter from herpes simplex virus
AU5120796A (en) DNA fragment, recombination vector containing the same and method for expressing foreign genes using the same
JPS57206700A (en) Structural gene in transformed microbial host cell and structural gene coding various allele and maturity form of preprothaumatin recombined cloning vehicle
RU94046362A (en) Dna sequences, plasmids, plasmid using, plants, bacteria, dna sequence using, yeast strains
ATE129286T1 (en) CLONING OF STREPTOMYCES AVERMITILIS GENES FOR BIOSYNTHESIS OF AVERMECTIN AND METHOD FOR USE THEREOF.
Schmitt et al. Complementation of transposition functions encoded by transposons Tn501 (HgR) and Tn1721 (TetR)
US4492758A (en) Hybrid vector and process for improving the amplification and expression of hybrid vectors by the use of mitochondrial DNA
HU9200680D0 (en) Process for the genetical transformation of myxobacteria
Lu et al. Cloning and organization of genes for 5S ribosomal RNA in the sea urchin, Lytechinus variegatus
Raab et al. Cell free synthesis of bacteriophage lambda replication proteins
Hohn et al. Ten years of cosmids
AU1671199A (en) Nucleic acids involved in the responder phenotype and applications thereo
Manresa et al. Amplification of the gene for DNA polymerase I by the multiplication of pol A in E. coli WK 6. Obtention and purification of the enzyme.
JPH0322157B2 (en)

Legal Events

Date Code Title Description
FG Patent granted

Ref document number: 117388

Country of ref document: FI

MA Patent expired