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ES2126743T3 - Alfa-amilasa oxidativamente estable. - Google Patents

Alfa-amilasa oxidativamente estable.

Info

Publication number
ES2126743T3
ES2126743T3 ES94909609T ES94909609T ES2126743T3 ES 2126743 T3 ES2126743 T3 ES 2126743T3 ES 94909609 T ES94909609 T ES 94909609T ES 94909609 T ES94909609 T ES 94909609T ES 2126743 T3 ES2126743 T3 ES 2126743T3
Authority
ES
Spain
Prior art keywords
alpha
amylases
amylase
mutant
altered
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94909609T
Other languages
English (en)
Other versions
ES2126743T5 (es
Inventor
Richard L Antrim
Christopher C Barnett
Colin Mitchinson
Scott D Power
Carol A Requadt
Leif P Solheim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21776903&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES2126743(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of ES2126743T3 publication Critical patent/ES2126743T3/es
Publication of ES2126743T5 publication Critical patent/ES2126743T5/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Materials For Medical Uses (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

SE PRESENTAN NUEVAS MUTACIONES DE LA ALFA-AMILASA DERIVADAS DE SECUENCIAS DE DNA DE ALFA-AMILASAS QUE SE PRODUCEN NATURALMENTE O RECOMBINANTES. LAS ALFA-AMILASAS MUTANTES, EN GENERAL, SE OBTIENEN MEDIANTE MODIFICACIONES IN VIVO DE UNA SECUENCIA PRECURSORA DE DNA QUE CODIFICA LA ALFA-AMILASA QUE SE PRODUCE NATURALMENTE O RECOMBINANTE PARA GENERAR LA SUSTITUCION (REEMPLAZAMIENTO) O LA ELIMINACION DE UNO O MAS RESIDUOS DE AMINOACIDO OXIDABLES EN LA SECUENCIA DE AMINOACIDO DE UNA ALFAAMILASA PRECURSORA. DICHAS ALFA-AMILASAS MUTANTES TIENEN ESTABILIDAD OXIDATIVA ALTERADA Y/O PERFILES DE RENDIMIENTO DE PH ALTERADOS Y/O ESTABILIDAD TERMICA ALTERADA COMPARADOS CON EL PRECURSOR. TAMBIEN SE PRESENTA COMPOSICIONES DE DETERGENTES Y DE LICUEFACCION DEL ALMIDON QUE COMPRENDEN LAS AMILASAS MUTANTES, ASI COMO METODOS PARA USAR LAS AMILASAS MUTANTES.
ES94909609T 1993-02-11 1994-02-10 Alfa-amilasa oxidativamente estable. Expired - Lifetime ES2126743T5 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16395 1987-02-19
US1639593A 1993-02-11 1993-02-11

Publications (2)

Publication Number Publication Date
ES2126743T3 true ES2126743T3 (es) 1999-04-01
ES2126743T5 ES2126743T5 (es) 2010-02-05

Family

ID=21776903

Family Applications (2)

Application Number Title Priority Date Filing Date
ES98109967T Expired - Lifetime ES2198617T5 (es) 1993-02-11 1994-02-10 Alfa-amilasa estable a la oxidación.
ES94909609T Expired - Lifetime ES2126743T5 (es) 1993-02-11 1994-02-10 Alfa-amilasa oxidativamente estable.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES98109967T Expired - Lifetime ES2198617T5 (es) 1993-02-11 1994-02-10 Alfa-amilasa estable a la oxidación.

Country Status (20)

Country Link
US (3) US6297037B1 (es)
EP (2) EP0689589B2 (es)
JP (1) JPH08506491A (es)
KR (1) KR100322793B1 (es)
CN (1) CN1104499C (es)
AT (2) ATE239075T1 (es)
AU (1) AU682863C (es)
BR (1) BR9405720A (es)
CA (1) CA2155831C (es)
CZ (1) CZ293163B6 (es)
DE (2) DE69432610T3 (es)
DK (2) DK0867504T4 (es)
ES (2) ES2198617T5 (es)
FI (2) FI119694B (es)
HU (1) HU219675B (es)
NO (1) NO307187B1 (es)
NZ (1) NZ262623A (es)
PL (1) PL310326A1 (es)
PT (1) PT867504E (es)
WO (1) WO1994018314A1 (es)

Families Citing this family (759)

* Cited by examiner, † Cited by third party
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