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EP0076222A1 - Automatische Pipettiereinrichtung - Google Patents

Automatische Pipettiereinrichtung Download PDF

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Publication number
EP0076222A1
EP0076222A1 EP82440027A EP82440027A EP0076222A1 EP 0076222 A1 EP0076222 A1 EP 0076222A1 EP 82440027 A EP82440027 A EP 82440027A EP 82440027 A EP82440027 A EP 82440027A EP 0076222 A1 EP0076222 A1 EP 0076222A1
Authority
EP
European Patent Office
Prior art keywords
pipette
valve
actuator
block
sleeve
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP82440027A
Other languages
English (en)
French (fr)
Other versions
EP0076222B1 (de
Inventor
Jean Bajard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to AT82440027T priority Critical patent/ATE18363T1/de
Publication of EP0076222A1 publication Critical patent/EP0076222A1/de
Application granted granted Critical
Publication of EP0076222B1 publication Critical patent/EP0076222B1/de
Expired legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0203—Burettes, i.e. for withdrawing and redistributing liquids through different conduits

Definitions

  • the present invention relates to the field of handling volumes of liquids for operations such as taking samples or dispensing reagents or dilutions, and relates to an automatic pipetting device intended for this purpose.
  • a pipette in particular of the bulb type, to which are added two photoelectric detectors signaling the arrival of the meniscus at a high position and at a low position, a three-way valve and two positions and a piping and reservoir system allowing circulation of the liquid by gravity, alternately to fill the pipette from a reservoir, in one position of the valve, then to empty said pipette in another container, in the other position the valve, the filling and emptying of the pipette being checked by checking the position of the meniscus by means of the detectors.
  • the present invention aims to overcome these drawbacks.
  • an automatic pipetting device characterized in that it is constituted by an automatic pipetting actuator and by a pipette block forming two dissociable sub-assemblies, in that the pipette block is mounted in the actuator removably, even in the filled state, while remaining sealed, and is constituted by a pipette connected at its lower end to a three-way valve and three positions, provided with a member training, and who is extended by piping elements, one of which leads to a product reservoir, the pipette being provided at its upper end with a sealed pneumatic connection device also serving for positioning the pipette, and in that the automatic actuator pipetting system is constituted by a stepping motor provided with a coupling for driving the valve of the pipette block, by a photoelectric meniscus detection assembly comprising a mobile detector driven in translation, by a pneumatic device d 'suction and discharge connected to the pipette, by an electronic control unit provided with a control and display panel, and by a quick
  • the automatic pipetting device is essentially constituted by an automatic actuator pipetting 1 and by a pipette block 2, which form two dissociable sub-assemblies.
  • the pipette unit 2 is removably mounted in the actuator 1 and is formed by a pipette 3 connected at its lower end to a three-way, three-position valve 4, provided with a drive member 5, which is extended by piping elements 6, one of which leads to a product reservoir 7.
  • a sealed pneumatic connection device 8 which is also used for its positioning.
  • the automatic pipetting actuator 1 consists of a stepping motor 9 provided with a withdrawable coupling 10 for driving the valve 4 of the pipette block 2, by a set 11 of photoelectric detection of the position of the meniscus, by a pneumatic suction and delivery device 12 connected to the pipette 3, by an electronic control unit 13 provided with a control and display panel 14, and by a lever 15 for rapid blocking of the pipette block 2.
  • the valve 4 is housed in a valve body 16 used for centering the pipette 3 and having openings 17 for connecting the pipe elements 6 (FIG. 3), and is constituted by a base 18 (FIG. 4) having the form d '' a disc and comprising, on one face around its center, a raised part 19 with a perfectly flat and polished surface forming a first sealing part, and in which three channels 20, 21 of which perpendicularly end, lead to the opposite face of the disc, and the third of which, 21, is connected to a perpendicular channel 22 ending in a cut plane 23 at the periphery of the disc 18 for the connection of the pipette 3, by a rotary valve 24 forming the second piece of waterproof and comprising also a perfectly flat and polished face, in which is provided a cell 25 of curved shape (FIG. 5).
  • the channels 20, 21 are advantageously arranged at 120 ° from each other, and the cell 25 has an angle at the center, enclosing its ends, of 120 °.
  • the ends of the cell 25 can be superimposed exactly on the orifices of the channel 21 and of one of the channels 20 in one of its rotational positions, or on the orifices channel 21 and the other channel 20 in the other position of rotation, no connection being established in the middle position of the valve 24 and the channels 20, 21 being closed in a sealed manner in this position.
  • the valve 24 On the face opposite to that carrying the cell 25, the valve 24 is provided with a central contact surface 26 and two or more recesses 27 distributed at regular intervals on the periphery, these recesses cooperating with drive pins 28 integral with a part forming the coupling member 5 so as to produce a peripheral drive, this part 5 being pressed punctually in its center on the surface 26, one or the other of these two parts in contact having for this purpose a domed shape, and being loaded by tension springs 29 by means of a transverse key 30, or the like, this part 5 being, moreover, guided in a sleeve 31 for mounting in the body 16, where it is immobilized by means of a pressure screw 32, or a similar securing device, the assembly of springs 29 and transverse key 30 forming an automatic return device in the middle position of the valve 4.
  • the coupling member 5 is provided with a transverse slot 33, arranged along the vertical axis of the pipette and per putting its drive link with the coupling 10 of the motor 9.
  • valve 4 a perfect seal is obtained between its constituent elements and its rapid rotation drive can be carried out without overturning effect, the bearing force being applied to the center of the valve 24 without requiring a very precise orientation of the drive member 5.
  • the provision of at least two clearances 27 for training, or a higher number of these clearances arranged at regular intervals helps to avoid a couple of overturning, these clearances 27 and the corresponding pins 28 also having the effect of maintaining the coaxiality between valve 24 and base 18.
  • the base 18 and the valve 24 are advantageously made of a material resistant to corrosion by chemical agents, and they are dimensioned in such a way that the joint widths make it possible to obtain the internal and external seals essential in all positions.
  • the valve body 16 Perpendicular to the drive member 5, the valve body 16 is provided with a bore 34 allowing the positioning of the pipette 3 by means of pins 35 cooperating with a circular groove of a metallic cylindrical sleeve 36 fixed on the lower end of the pipette 3 coaxial therewith and resting on the cut plane 23 of the base 18 of the valve 4 with the interposition of a compressed seal 37, this sleeve always having the same outside diameter, whatever the outside diameter of the pipette and the caliber of the pipette. Thanks to this embodiment, the pipette 3 is secured to the valve body 16 without residual play, and the positioning of the block 16 will automatically cause that of the pipette 3.
  • the sealed pneumatic connection device 8 fitted to the upper end of the pipette 3 is in the form of a sleeve similar to that fitted to the lower part of the pipette 3, this sleeve cooperating, on the one hand, with a guide bore (not shown) positioned in the actuator 1 and, on the other hand, with a compressible seal connected to the pneumatic device 12 for suction and discharge.
  • This device 12 allows the movement of the liquids handled by vacuum for the suction and by relative overpressure for the discharge, that is to say the distribution of the liquids.
  • any pipette can be mounted, regardless of its size, in any valve body to form a pipette block of constant size, and all the pipette blocks 2 thus formed can be fitted perfectly interchangeably in the actuator 1.
  • the pipettes 3 used are constituted by calibrated calibrated tubes, that is to say with constant section over their entire length, provided with two graduation lines engraved at a distance from each other corresponding to the nominal volume of the pipette.
  • the internal diameters of these tubes may vary slightly, the distances separating the lines can therefore also vary, so that the actuator 1 must be equipped with a device taking account of this possible variation.
  • the assembly 11 for photoelectric detection of the position of the meniscus is arranged in the actuator 1 parallel to the pipette 3 and is constituted by a lower fixed detector 38 at the level of which the lower graduation lines are arranged. 3e all pipettes 3, and by an upper mobile detector 39 displaceable by means of a screw-nut assembly 40, or similar device, by a stepping motor 41, which is controlled by the electronic control assembly 13 as a function of data relating to the calibration of the block - pipette 2 considered, composed on the control and display panel 14.
  • the lower detector 38 is held in abutment at the lower graduation lines of the pipettes 3 by means of an elastic device with springs, or the like, and can be retracted under the action of a push from the upper mobile detector 39 when the latter is moved down until it reaches said level of the lower graduation lines of the pipettes 3, so that the entire useful or nominal volume of the pipette 3 can be used as a burette.
  • the electronic control assembly 13 is provided with calculation means, which make it possible to determine for the pipette block 2 considered intermediate volumes as a function of requirements.
  • the lever 15 for rapid locking of the pipette block 2 is advantageously an eccentric, or elastically loaded, lever simultaneously applying sealed from the upper end of the pipette 3 against the guide bore of the actuator 1, the clutch between the drive member 5 of the valve 4 and the coupling 10 of the stepping motor 9 , and the locking in position of the pipette block 2 in the actuator 1 with simultaneous alignment of the pipette 3 with the axis of the detectors 38 and 39, an unlocking of the lever 15 having the effect of instantly releasing the pipette block 2 for its possible replacement by another.
  • the pipette block 2 can be arranged, together with other pipette blocks, on an alternative or continuous, circular or linear transport device, of the type known, its establishment and maintenance in the actuator 1, as well as its removal being carried out automatically by said transport device by means of a jack, or the like.
  • the pipette block 2 being able to easily and quickly be interchanged, and the program for the new pipette block in place can be dialed using its distinctive number.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Separation By Low-Temperature Treatments (AREA)
  • Cyclones (AREA)
  • Centrifugal Separators (AREA)
  • Beverage Vending Machines With Cups, And Gas Or Electricity Vending Machines (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
EP82440027A 1981-09-23 1982-09-22 Automatische Pipettiereinrichtung Expired EP0076222B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT82440027T ATE18363T1 (de) 1981-09-23 1982-09-22 Automatische pipettiereinrichtung.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR8118065 1981-09-23
FR8118065A FR2513376A1 (fr) 1981-09-23 1981-09-23 Dispositif de pipetage automatique

Publications (2)

Publication Number Publication Date
EP0076222A1 true EP0076222A1 (de) 1983-04-06
EP0076222B1 EP0076222B1 (de) 1986-03-05

Family

ID=9262446

Family Applications (1)

Application Number Title Priority Date Filing Date
EP82440027A Expired EP0076222B1 (de) 1981-09-23 1982-09-22 Automatische Pipettiereinrichtung

Country Status (6)

Country Link
US (1) US4484696A (de)
EP (1) EP0076222B1 (de)
JP (1) JPS5866854A (de)
AT (1) ATE18363T1 (de)
DE (1) DE3269648D1 (de)
FR (1) FR2513376A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6332607B1 (en) 1997-12-31 2001-12-25 Neopost B.V. Apparatus and method for separating sheets from a stack that includes a pulsed suction assembly

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050269368A1 (en) * 2004-05-14 2005-12-08 Proulx Stephen P Fluid dispenser cartridge with bladder means
DE102005025640A1 (de) * 2005-06-03 2006-12-07 Scienion Ag Mikrodispenser und zugehöriges Betriebsverfahren
CN103105316A (zh) * 2012-11-06 2013-05-15 苏州聚阳环保科技有限公司 一种注射泵

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2005374A1 (de) * 1968-04-02 1969-12-12 Battelle Development Corp
US3491368A (en) * 1968-05-10 1970-01-20 Hewlett Packard Co Automatic dispensing system with fluid purging means
DE2330499A1 (de) * 1973-06-15 1975-01-09 Boepple & Co Kg Justieranlage fuer pipetten und bueretten

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1705940A (en) * 1927-06-16 1929-03-19 Stockbridge & Borst Apparatus for dispensing liquids
NL252663A (de) * 1959-06-18
US3143393A (en) * 1959-06-18 1964-08-04 Luc Donald De Seguin Des Hons Apparatus for automatically performing chemical operations and similar or related operations
US3193141A (en) * 1963-08-23 1965-07-06 Swift & Co Device for measuring and dispensing variable amounts of liquid
US3206072A (en) * 1964-09-22 1965-09-14 Calvin R Mencken Pipetting machine
SE327840B (de) * 1968-01-25 1970-08-31 Atuokemi Ab
DK143719C (da) * 1979-01-03 1982-03-08 Radiometer As Fremgangsmaade til udluftning af en vaeskedoserende stempelpumpe og stempelpumpe med et arrangement til brug ved udoevelse affremgangsmaaden

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2005374A1 (de) * 1968-04-02 1969-12-12 Battelle Development Corp
US3491368A (en) * 1968-05-10 1970-01-20 Hewlett Packard Co Automatic dispensing system with fluid purging means
DE2330499A1 (de) * 1973-06-15 1975-01-09 Boepple & Co Kg Justieranlage fuer pipetten und bueretten

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6332607B1 (en) 1997-12-31 2001-12-25 Neopost B.V. Apparatus and method for separating sheets from a stack that includes a pulsed suction assembly

Also Published As

Publication number Publication date
JPS5866854A (ja) 1983-04-21
DE3269648D1 (en) 1986-04-10
EP0076222B1 (de) 1986-03-05
FR2513376A1 (fr) 1983-03-25
ATE18363T1 (de) 1986-03-15
FR2513376B1 (de) 1983-12-09
US4484696A (en) 1984-11-27

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